A cell culture device

Abstract

The present invention relates to the field of biological culture technology, specifically, a cell culture device. It includes a culture box and a culture dish, including a support frame and a lighting column inside the culture box, the support frame is used to support the culture dish, the culture dish is made of transparent material, the side elevation of the lighting column is provided with a plurality of connection ports, the connection ports are used to accommodate the corners of the culture dish; a scattered light source is connected to the top of the lighting column, the lighting column includes a reflective layer and a refractive layer, the refractive layer is fitted to the inner wall of the reflective layer, the irradiation end of the scattered light source is located on the inner side of the refractive layer, and an inclined light guide plate is provided on the side of the refractive layer opposite to the connection port, and the light guide plate reflects the light source into the connection port. The lighting column provides illumination to multiple culture dishes, so that the illumination intensity of the multiple culture dishes is the same, ensuring that different culture dishes maintain the same culture conditions, and facilitating the efficient cultivation of microalgae or other substances that require illumination.

Description

Technical Field

[0001] The invention relates to the technical field of biological culture, in particular to a cell culture device. Background Art

[0002] Microalgae cells contain: proteins, lipids, algae polysaccharides, β-carotene, various inorganic elements (such as Cu, Fe, Se, Mn, Zn, etc.) and other high-value nutrients and chemical raw materials. Microalgae has a high protein content and is an important source of single-cell protein (SCP). Microalgae contains vitamin A, vitamin E, thiamine, riboflavin, pyridoxine, vitamin B12, vitamin C, biotin, inositol, folic acid, calcium pantothenate and niacin, which increase its value as SCP. Algae have a high content of carotenoids, which have coloring and nutritional effects. They can be used to prevent and treat cancer, resist radiation, delay aging, enhance the body's immunity and other physiological effects. In order to carry out Fang Bonni's in-depth research on microalgae, microalgae are often cultured in cell culture devices in the laboratory.

[0003] In the prior art, light source is generally provided for microalgae culture by top lighting. A single directional light source easily causes the culture dish at the bottom to receive weaker light, and the lighting conditions of multiple culture dishes are different, which affects the culture effect of microalgae.

[0004] Therefore, the present application provides a cell culture device to solve the problem in the prior art that the illumination intensity of multiple culture dishes in the microalgae culture process is different, which affects the microalgae culture effect. Summary of the invention

[0005] In order to solve the above problems, the present invention provides a cell culture device.

[0006] The technical solution adopted by the present invention to solve the technical problem is: a cell culture device, including a culture box and a culture dish, including a support frame and a lighting column arranged inside the culture box, the support frame is used to support the culture dish, the culture dish is made of a transparent material, and the side elevation of the lighting column is provided with a plurality of connection ports, the connection ports are used to accommodate the corners of the culture dish;

[0007] A scattered light source is connected to the top of the lighting column, which includes a reflective layer and a refractive layer. The refractive layer is adhered to the inner wall of the reflective layer, and the irradiation end of the scattered light source is located on the inner side of the refractive layer. An inclined light guide plate is provided on the side of the refractive layer opposite to the connecting port, and the light guide plate reflects the light source into the connecting port.

[0008] As an optimization, the incubator includes a box body and a door, the door is rotatably connected to the box body, and the lighting columns include two fixed lighting columns and two movable lighting columns, the two fixed columns are fixed to the inside of the box body, and the two movable lighting columns are fixed to the inside of the door.

[0009] As an optimization, the support frame is fixed to the inner side of the incubator, and a plurality of support ribs are fixed to the side of the support frame, and the support ribs are used to support the culture dish.

[0010] As an optimization, the reflective layer and the refractive layer are both cylindrical, the reflective layer is made of an opaque material, and the connecting port penetrates the reflective layer and the refractive layer;

[0011] The inner side surface of the refraction layer is provided with a plurality of refraction concave points, and the refraction concave points make the light source reflect back and forth continuously on the inner side surface of the lighting column.

[0012] As an optimization, the lower end of the light guide plate is inclined toward the culture dish, and multiple light guide plates are arranged in one-to-one correspondence with multiple connection ports.

[0013] As an optimization, the corners of the culture dish are recessed to form a connecting arc, and the connecting arc can be inserted into the inner side of the connecting port.

[0014] The present invention provides a cell culture device, which has the following advantages:

[0015] The cell culture incubator of the present application is provided with an illumination column inside, and the corners of the culture dishes are connected to the illumination column. The illumination column provides illumination to multiple culture dishes, so that the illumination intensity of the multiple culture dishes is the same, thereby ensuring that different culture dishes maintain the same culture conditions, and facilitating the efficient cultivation of microalgae or other substances that require light. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is an axial schematic diagram of the present invention.

[0017] Figure 2 It is a schematic diagram of the axial side of the box door of the present invention.

[0018] Figure 3 It is a schematic diagram of the connection structure of the culture dish and the lighting column of the present invention.

[0019] Figure 4 It is a schematic diagram of the axial side of the box body of the present invention.

[0020] Figure 5 It is a schematic diagram of the axial side of the lighting column of the present invention.

[0021] Figure 6 It is a schematic axial view of the bottom of the lighting column of the present invention.

[0022] Figure 7 It is a schematic front view of the lighting column of the present invention.

[0023] Figure 8 For the present invention Figure 6 Schematic diagram of the AA section structure.

[0024] Among them, 1. box body, 2. box door, 3. support frame, 4. culture dish, 5. connection port, 6. scattered light source, 7. lighting column, 8. reflection layer, 9. refraction layer, 10. light guide plate, 11. support ribs. DETAILED DESCRIPTION

[0025] In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inside", "outside", etc. indicate positions or positional relationships based on the positions or positional relationships shown in the accompanying drawings, or the positions or positional relationships in which the product of the invention is usually placed when in use. They are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific position, be constructed and operated in a specific position, and therefore cannot be understood as limiting the present invention. In addition, the terms "first", "second", "third", etc. are only used to distinguish the description, and cannot be understood as indicating or implying relative importance.

[0026] The present solution also includes a controller, the position of which is set by the staff according to actual conditions during operation. The controller is used to control the electrical devices used in the present solution, including but not limited to sensors, motors, telescopic rods, water pumps, solenoid valves, heating wires, heat pumps, display screens, computer input devices, switch buttons, communication equipment, lights, speakers and microphones; the controller is an Intel processor, an AMD processor, a PLC controller, an ARM processor or a single-chip microcomputer, and the supporting components include a motherboard, a memory stick, a storage medium and a power supply, and the power supply is AC power or a lithium battery; when a display screen is provided, a display card is also provided.

[0027] like Figure 1-8 As shown, a cell culture device includes a culture box and a culture dish 4, wherein a support frame 3 and a lighting column 7 are provided inside the culture box, wherein the support frame 3 is used to support the culture dish 4, wherein the culture dish 4 is made of a transparent material, and a plurality of connection ports 5 are provided on the side elevation of the lighting column 7, wherein the connection ports 5 are used to accommodate the corners of the culture dish 4;

[0028] A scattered light source 6 is connected to the top of the lighting column 7. The lighting column 7 includes a reflective layer 8 and a refractive layer 9. The refractive layer 9 is adhered to the inner wall of the reflective layer 8. The irradiation end of the scattered light source 6 is located on the inner side of the refractive layer 9. An inclined light guide plate 10 is provided on the side of the refractive layer 9 opposite to the connecting port 5. The light guide plate 10 reflects the light source into the connecting port 5.

[0029] A transparent lens can be set on the top of the lighting column 7 so that the light source can completely pass through the transparent lens into the interior of the lighting column 7. The reflective layer 8 can reflect the light source toward the interior of the lighting column 7. The setting of the refractive layer 9 allows the light source to converge on the light guide plate 10 and be introduced into the connecting port 5 through the light guide plate 10.

[0030] When the culture dish 4 is connected to the connection port 5 , the culture dish 4 can be in contact and sealed with the main body of the lighting column 7 .

[0031] like Figure 1 and Figure 2 As shown, the incubator includes a box body 1 and a door 2, the door 2 is rotatably connected to the box body 1, and the lighting column 7 includes two fixed lighting columns 7 and two movable lighting columns 7, the two fixed columns are fixed inside the box body 1, and the two movable lighting columns 7 are fixed on the inner side of the door 2.

[0032] like Figure 3 As shown, the support frame 3 is fixed to the inner side of the incubator, and a plurality of support ribs 11 are fixed to the side of the support frame 3 , and the support ribs 11 are used to support the culture dish 4 .

[0033] The supporting rib 11 is flush with the bottom of the connecting port 5 . When the culture dish 4 is placed, the corner of the culture dish 4 is connected to the connecting port 5 .

[0034] like Figure 5 and Figure 8 As shown, the reflective layer 8 and the refractive layer 9 are both cylindrical, the reflective layer 8 is made of an opaque material, and the connecting port 5 penetrates the reflective layer 8 and the refractive layer 9;

[0035] The inner side surface of the refractive layer 9 is provided with a plurality of refractive concave points, and the refractive concave points make the light source reflect back and forth continuously on the inner side surface of the lighting column 7 .

[0036] The refractive layer 9 can make the light source on the upper side tilt downward, and make the light source on the upper side of the light guide plate 10 tilt downward to the light guide plate 10 and its surroundings, and then the light source is guided by the light guide plate 10 and enters the connection port 5 .

[0037] like Figure 8 As shown, the lower end of the light guide plate 10 is inclined toward the culture dish 4 , and a plurality of light guide plates 10 are arranged in one-to-one correspondence with a plurality of connection ports 5 .

[0038] The corners of the culture dish 4 are recessed to form a connecting arc, and the connecting arc can be inserted into the inner side of the connecting port 5 .

[0039] Directions:

[0040] When the device is used, the microalgae cells are placed on the culture dishes 4, the culture dishes 4 are placed inside the box 1, and the adjacent culture dishes 4 are divided by the support ribs 11;

[0041] One end of the culture dish 4 is connected to the two lighting columns 7 inside the box 1, and the corner of the culture dish 4 is in contact and sealed with the connecting port 5. The box door 2 is closed, so that the lighting column 7 on the box door 2 is connected to the outer end of the culture dish 4;

[0042] Turn on the scattered light source 6, and the light enters the interior of the lighting column 7;

[0043] The light is reflected by the reflective layer 8 toward the axial direction of the lighting column 7, and the refractive layer 9 refracts the light toward the light guide plate 10;

[0044] The light is guided by the guide plate and then irradiated into the connection port 5 to irradiate the culture dish 4 . The four lighting columns 7 provide light uniformly through the four corners of the culture dish 4 .

[0045] The above-mentioned specific embodiments are only specific cases of the present invention. The patent protection scope of the present invention includes but is not limited to the product form and style of the above-mentioned specific embodiments. Any cell culture device that conforms to the claims of the present invention and any appropriate changes or modifications made thereto by ordinary technicians in the corresponding technical field shall fall within the patent protection scope of the present invention.

Claims

1. A cell culture device, comprising a culture box and a culture dish (4), characterized in that: The incubator comprises a support frame (3) and a lighting column (7) arranged inside, wherein the support frame (3) is used to support a culture dish (4), wherein the culture dish (4) is made of a transparent material, and a plurality of connection ports (5) are provided on the side elevation of the lighting column (7), wherein the connection ports (5) are used to accommodate the corners of the culture dish (4); A scattered light source (6) is connected to the top of the lighting column (7), the lighting column (7) comprises a reflective layer (8) and a refractive layer (9), the refractive layer (9) is arranged in contact with the inner wall of the reflective layer (8), the irradiation end of the scattered light source (6) is located on the inner side of the refractive layer (9), and an inclined light guide plate (10) is arranged on the side of the refractive layer (9) opposite to the connecting port (5), and the light guide plate (10) reflects the light source into the connecting port (5); The reflective layer (8) and the refractive layer (9) are both cylindrical, the reflective layer (8) is made of a light-proof material, and the connecting port (5) penetrates the reflective layer (8) and the refractive layer (9); The inner side surface of the refractive layer (9) is provided with a plurality of refractive concave points, and the refractive concave points cause the light source to continuously reflect back and forth on the inner side surface of the lighting column (7).

2. A cell culture device according to claim 1, characterized in that: The incubator comprises a box body (1) and a box door (2), wherein the box door (2) is rotatably connected to the box body (1), and the lighting column (7) comprises two fixed lighting columns (7) and two movable lighting columns (7), wherein the two fixed columns are fixed inside the box body (1), and the two movable lighting columns (7) are fixed inside the box door (2).

3. A cell culture device according to claim 1, characterized in that: The support frame (3) is fixed to the inner side of the culture box, and a plurality of support ribs (11) are fixed to the side of the support frame (3), and the support ribs (11) are used to support the culture dish (4).

4. A cell culture device according to claim 1, characterized in that: The lower end of the light guide plate (10) is inclined toward the culture dish (4), and a plurality of light guide plates (10) and a plurality of connection ports (5) are arranged in a one-to-one correspondence.

5. A cell culture device according to claim 1, characterized in that: The corners of the culture dish (4) are recessed to form a connecting arc, and the connecting arc can be inserted into the inner side of the connecting port (5).

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