A Chinese medicine composition for treating liver cancer and its preparation method

Abstract

The invention belongs to the field of medical technology, and specifically relates to a Chinese medicine composition for treating liver cancer and a preparation method thereof. The Chinese medicine composition is composed of the following raw materials in parts by weight: 20-28 parts of kiwifruit root, 17-22 parts of lotus root, 12-15 parts of golden thread orchid, 7-10 parts of knotweed, 1-5 parts of cubeb, 5-10 parts of astragalus, 1-5 parts of quinquefolius, and 1-3 parts of liquorice. In this prescription, kiwi root clears away heat and detoxifies, promotes blood circulation and relieves pain; Baimai root replenishes deficiency and clears away heat, and the two ingredients are combined as the main medicine to strengthen the body and eliminate evil; Jinxianlan dispels wind and detoxifies, clears heat and cools blood; Polygonum cuspidatum disperses blood stasis and relieves pain, clears heat and detoxifies, and the two ingredients are combined as the assistant medicine to clear heat, nourish yin and remove blood stasis; Piper culmorum promotes qi and relieves pain, Astragalus invigorates qi and raises yang, and Prunus oleifera relaxes tendons and activates collaterals, and the three ingredients are adjuvants that invigorate qi and warm yang; Licorice harmonizes the various medicines, guides the medicines into the meridians, and jointly exerts the effects of removing blood stasis, softening hard masses, strengthening the body and eliminating evil, and warming the spleen and unblocking collaterals. The Chinese medicine composition can inhibit the growth of liver cancer cells without side effects.

Description

Technical Field

[0001] The invention belongs to the technical field of medicine, and particularly relates to a traditional Chinese medicine composition for treating liver cancer and a preparation method thereof. Background Art

[0002] Malignant tumors have the characteristics of high mortality, short survival, and poor quality of life. Liver cancer, or malignant tumors of the liver, is the sixth most common cancer and the fourth leading cause of cancer death, posing a serious threat to human health. Current treatments for liver cancer include: resection, radiotherapy, chemotherapy, intervention, targeted drugs, immunotherapy, and traditional Chinese medicine treatment, among which resection is the main treatment. However, due to the high early concealment of liver cancer cells and their rapid progression, most liver cancer patients are already in the middle and late stages when they are discovered. In addition, liver cancer cell lesions are multiple and easy to spread, so less than 30% of liver cancer patients are eligible for liver cancer resection. Clinical studies have found that surgical resection is often accompanied by many complications and poor prognosis.

[0003] Research has found that comprehensive treatment based on traditional Chinese medicine is of great significance in controlling the progression of liver cancer patients, improving their quality of life, and prolonging their survival. Chinese medicine prescriptions have high effectiveness and safety in clinical treatment and tumor prevention, and therefore have attracted much attention in the medical field. The anti-liver cancer effect of existing Chinese medicine prescriptions is unclear, so there is a high demand for new drugs that can effectively treat liver cancer in this field, in order to achieve effective treatment of liver cancer. Based on this, it is urgent to provide a Chinese medicine composition for the treatment of liver cancer with more stable and significant efficacy. Summary of the invention

[0004] The first purpose of the present invention is to provide a Chinese medicine composition for treating liver cancer, which can inhibit the growth of liver cancer cells and has no side effects.

[0005] The second purpose of the present invention is to provide a method for preparing a Chinese medicine composition for treating liver cancer, which has simple steps and is easy to operate.

[0006] In order to achieve the above object, the technical solution adopted by the present invention is:

[0007] A traditional Chinese medicine composition for treating liver cancer, the traditional Chinese medicine composition is composed of the following raw materials in parts by weight: 20-28 parts of kiwifruit roots, 17-22 parts of lotus roots, 12-15 parts of roxburghii, 7-10 parts of knotweed, 1-5 parts of cubeb, 5-10 parts of astragalus, 1-5 parts of quinquefolius, and 1-3 parts of liquorice.

[0008] Furthermore, the Chinese medicine composition is composed of the following raw materials in parts by weight: 25 parts of kiwi root, 19 parts of lotus root, 13 parts of roxburghii, 8 parts of knotweed, 3 parts of cubeb, 7 parts of astragalus, 4 parts of quinquefolius, and 2 parts of licorice.

[0009] Furthermore, the kiwifruit root is an Actinidia chinensis root.

[0010] The preparation method of the above-mentioned Chinese medicine composition comprises the following steps:

[0011] (1) Weighing the raw materials according to the above weight parts and mixing them evenly, soaking and decocting them in water, and filtering them after decocting to obtain a first filtrate;

[0012] (2) soaking and decocting the residue after filtering the first medicinal liquid in step (1), filtering after decocting to obtain a second filtrate;

[0013] (3) The first filtrate and the second filtrate are combined and concentrated to an extract with a relative density of 1.0-1.1.

[0014] Furthermore, the amount of water used in steps (1) and (2) is 20-30 times the total weight of the raw material.

[0015] Furthermore, the soaking time in step (1) is 2-3 hours, and the boiling time is 3-4 hours; the soaking time in step (2) is 3-4 hours, and the boiling time is 3-4 hours.

[0016] Furthermore, auxiliary materials are added to the traditional Chinese medicine composition to prepare a corresponding dosage form.

[0017] Furthermore, the dosage form is an oral dosage form.

[0018] Furthermore, the oral dosage form is oral liquid, tablet, powder, granule or capsule.

[0019] The pharmacological and medicinal properties of the raw materials used in the present invention are analyzed as follows:

[0020] Actinidia chinensis root: [Nature and flavor] Sweet, astringent, cool in nature. Enters the heart, kidney, liver, and spleen meridians. [Functions and indications] Clears away heat and detoxifies, dispels wind and dampness, activates blood circulation and reduces swelling; mainly used to treat hepatitis, indigestion, edema, boils, scrofula, tuberculosis, gastrointestinal tumors, and breast cancer.

[0021] Baimai root: [Nature and flavor] Sweet, bitter, slightly cold. Enters the lung meridian. [Functions and indications] Tonifies deficiency, clears heat, quenches thirst; Indications: Consumption, fatigue, fever due to yin deficiency, dry throat, thirst, etc. "Tang Materia Medica" states: "It can relieve qi, quench thirst, remove heat, eliminate deficiency, and replenish deficiency."

[0022] Golden thread orchid: [Nature and flavor and meridians] Sweet, neutral. Enters the lung, liver, kidney, and bladder meridians. [Functions and indications] Clears heat and cools blood, dispels wind and dampness, and detoxifies. Indications: sores, swellings, tumors, etc.

[0023] Polygonum cuspidatum: [Nature and flavor and meridians] Slightly bitter, slightly cold. Enters the liver, gallbladder, and lung meridians. [Functions and indications] It can dissipate dampness and reduce jaundice, clear away heat and detoxify, dissipate blood stasis and relieve pain, resolve phlegm and relieve cough. It is mainly used to treat gallstones, abnormal liver function, fatty liver, pain, etc.

[0024] Piper citrate: [Nature and flavor and meridians] Spicy, warm in nature. Enters the spleen, stomach, kidney, and bladder meridians. [Functions and indications] Warms the middle and dispels cold, promotes qi and relieves pain, and promotes bile secretion. Indications: stomach cold vomiting, cold pain in the abdomen, diarrhea, cholecystitis, gallstones, etc.

[0025] Astragalus: [Nature and flavor and meridians] Sweet, warm in nature. Enters the lung and spleen meridians. [Functions and indications] Invigorates Qi and raises Yang, consolidates the exterior and stops sweating, promotes diuresis and reduces swelling, removes toxins and discharges pus, and heals sores and promotes tissue regeneration. Indications: spleen deficiency, diarrhea, spontaneous sweating, edema and other symptoms of Qi deficiency and blood deficiency.

[0026] Five-fingered ginseng: [Nature and flavor] Sweet, neutral. Enters the spleen, lung, and liver meridians. [Functions and indications] Strengthens the spleen and lungs, promotes qi and eliminates dampness, relaxes muscles and activates collaterals. Indications: edema due to spleen deficiency, loss of appetite and weakness, cough due to tuberculosis, night sweats, leucorrhea, postpartum milklessness, irregular menstruation, rheumatic pain, edema, etc.

[0027] Licorice: [Nature and flavor] Sweet, neutral. Enters the spleen, stomach, heart, and lung meridians. [Functions and indications] Harmonizes the middle and relieves urgency, moistens the lungs, clears away heat and detoxifies, and harmonizes various medicines. Indications: shortness of breath, fatigue, loss of appetite, loose stools, cough, sore throat, etc. "Ben Jing" records: It can treat cold and heat evil spirits in the five internal organs, strengthen tendons and bones, grow muscles, double strength, wounds and swellings, and detoxify.

[0028] Compared with the prior art, the beneficial effects of the present invention are mainly:

[0029] The invention provides a Chinese medicine composition for treating liver cancer, wherein the root of Actinidia chinensis clears away heat and detoxifies, promotes blood circulation and relieves pain; the root of Radix Polygonati Nigrum replenishes deficiency and clears away heat, and the two ingredients are combined as the main medicine to strengthen the body and eliminate evil; the golden thread orchid dispels wind and detoxifies, clears heat and cools blood; the knotweed disperses blood stasis and relieves pain, clears heat and detoxifies, and the two ingredients are combined as the ministerial medicine to clear heat, nourish yin and remove blood stasis; the root of Piper ciliata promotes qi and relieves pain, the root of Astragalus invigorates qi and raises yang, and the root of Prunus mume relaxes tendons and activates collaterals, and the three ingredients are adjuvant medicines to invigorate qi and warm yang; and the root of Licorice harmonizes various medicines, guides the medicine into the meridians, and jointly exerts the effects of removing blood stasis, softening hard masses, strengthening the body and eliminating evil, and warming the spleen and unblocking collaterals. The above ingredients are used in combination, which can inhibit the growth of liver cancer cells without side effects, and provide a new idea for the treatment of liver cancer. DETAILED DESCRIPTION

[0030] The technical scheme of the present invention is further described below in conjunction with specific embodiments. However, it should be understood by those skilled in the art that the following examples are only used to illustrate the present invention and should not be regarded as limiting the present invention. The specific conditions not specified in the examples are carried out according to conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used, unless otherwise specified, are conventional products obtained through commercial channels.

[0031] Example

[0032] Example 1

[0033] The present embodiment provides a traditional Chinese medicine composition for treating liver cancer, which is composed of the following raw materials in parts by weight: 25 parts of Chinese kiwifruit roots, 19 parts of lotus roots, 13 parts of roxburghii, 8 parts of knotweed, 3 parts of cubeb, 7 parts of astragalus, 4 parts of quinquefolius, and 2 parts of licorice.

[0034] This embodiment also provides a method for preparing the above-mentioned Chinese medicine composition, and the specific preparation process is as follows:

[0035] (1) Weighing each raw material drug according to the above weight portion, mixing them evenly, and then placing them in a high-necked round-bottom flask, adding distilled water to the high-necked round-bottom flask according to a ratio of the amount of distilled water added to the total weight of the raw material drug of 25:1, soaking for 2.5 hours, and then boiling for 3.2 hours. After boiling, filtering the first drug liquid with 2 layers of gauze, 4 layers of gauze, 8 layers of gauze, and 16 layers of gauze in sequence to obtain a first filtrate;

[0036] (2) placing the residue after filtering the first medicinal liquid in step (1) into a high-necked round-bottom flask, adding an equal amount of distilled water as in step (1) to the high-necked round-bottom flask and soaking for 3.5 hours, boiling for 3.5 hours after soaking, and filtering the second medicinal liquid with 3 layers of gauze, 6 layers of gauze, 12 layers of gauze, and 20 layers of gauze in sequence after boiling to obtain a second filtrate;

[0037] (3) The first filtrate and the second filtrate are combined and concentrated to an extract with a relative density of 1.03.

[0038] Example 2

[0039] The present embodiment provides a traditional Chinese medicine composition for treating liver cancer, which is composed of the following raw materials in parts by weight: 20 parts of Chinese kiwifruit roots, 17 parts of lotus roots, 12 parts of roxburghii, 7 parts of knotweed, 1 part of cubeb, 5 parts of astragalus, 1 part of quinquefolius, and 1 part of licorice.

[0040] This embodiment also provides a method for preparing the above-mentioned Chinese medicine composition, and the specific preparation process is as follows:

[0041] (1) Weighing each raw material drug according to the above weight portion, mixing them evenly, and then placing them in a high-necked round-bottom flask, adding distilled water to the high-necked round-bottom flask according to a ratio of the amount of distilled water added to the total weight of the raw material drug of 20:1, soaking for 3 hours, and then boiling for 4 hours. After boiling, the first drug liquid is filtered through 2 layers of gauze, 4 layers of gauze, 8 layers of gauze, and 16 layers of gauze in sequence to obtain a first filtrate;

[0042] (2) placing the residue after filtering the first medicinal liquid in step (1) into a high-necked round-bottom flask, adding an equal amount of distilled water as in step (1) to the high-necked round-bottom flask to soak for 3 hours, boiling for 4 hours after soaking, and filtering the second medicinal liquid with 3 layers of gauze, 6 layers of gauze, 12 layers of gauze, and 20 layers of gauze in sequence after boiling to obtain a second filtrate;

[0043] (3) The first filtrate and the second filtrate are combined and concentrated to an extract with a relative density of 1.1.

[0044] Example 3

[0045] The present embodiment provides a traditional Chinese medicine composition for treating liver cancer, which is composed of the following raw materials in parts by weight: 28 parts of Chinese kiwifruit roots, 22 parts of lotus roots, 15 parts of roxburghii, 10 parts of knotweed, 5 parts of cubeb, 10 parts of astragalus, 5 parts of quinquefolius, and 3 parts of licorice.

[0046] This embodiment also provides a method for preparing the above-mentioned Chinese medicine composition, and the specific preparation process is as follows:

[0047] (1) Weighing each raw material drug according to the above weight portion, mixing them evenly, and then placing them in a high-necked round-bottom flask, adding distilled water to the high-necked round-bottom flask in a ratio of 30:1 to the total weight of the raw material drug, soaking them for 2 hours, and then boiling them for 3 hours. After boiling, filtering the first drug liquid with 2 layers of gauze, 4 layers of gauze, 8 layers of gauze, and 16 layers of gauze in sequence to obtain a first filtrate;

[0048] (2) placing the residue after filtering the first medicinal liquid in step (1) into a high-necked round-bottom flask, adding an equal amount of distilled water as in step (1) to the high-necked round-bottom flask to soak for 4 hours, boiling for 3 hours after soaking, and filtering the second medicinal liquid with 3 layers of gauze, 6 layers of gauze, 12 layers of gauze, and 20 layers of gauze in sequence after boiling to obtain a second filtrate;

[0049] (3) The first filtrate and the second filtrate are combined and concentrated to an extract with a relative density of 1.0.

[0050] Comparative Example

[0051] Comparative Example 1

[0052] This comparative example provides a Chinese medicine composition, which is composed of the following raw materials in parts by weight: 25 parts of Chinese kiwifruit roots, 13 parts of golden thread orchid, 8 parts of knotweed, 3 parts of cubeb, 7 parts of astragalus, 4 parts of quinquefolius, and 2 parts of liquorice, and the rest is the same as in Example 1.

[0053] Comparative Example 2

[0054] This comparative example provides a Chinese medicine composition, which is composed of the following raw materials in parts by weight: 25 parts of Chinese kiwifruit roots, 19 parts of lotus roots, 21 parts of knotweed, 3 parts of cubeb, 7 parts of astragalus, 4 parts of quinquefolius, and 2 parts of liquorice, and the rest is the same as in Example 1.

[0055] Comparative Example 3

[0056] This comparative example provides a Chinese medicine composition, which is composed of the following raw materials in parts by weight: 25 parts of Chinese kiwifruit roots, 19 parts of lotus roots, 21 parts of roxburghii, 3 parts of cubeb, 7 parts of astragalus, 4 parts of quinquefolius, and 2 parts of liquorice, and the rest is the same as in Example 1.

[0057] Effect example

[0058] Effect Example 1

[0059] This effect example explores the inhibitory effect of the Chinese medicine composition of Examples 1-3 and Comparative Examples 1-3 on Hep G2 tumor cells. The specific process is as follows:

[0060] The extracts of Examples 1-3 and Comparative Examples 1-3 were diluted to 10 mg / mL, 40 mg / mL, and 80 mg / mL using RPMI-1640 complete medium, and sterilized by filtration using a 0.25 μm sterile filter to obtain drug-containing medium for each group for later use. In addition to the drug-containing medium group, a negative control group without drug and a pure medium group were set up, and 5 replicates were set up for all groups.

[0061] Hep G2 cells were resuspended in RPMI-1640 complete medium and the cell density was adjusted to 5 × 10 4 / mL, and obtain Hep G2 cell suspension. The Hep G2 cell suspension was added to a 96-well plate, 100 μL per well, and cultured in a cell culture incubator at 37°C and 5% CO2 for 24 hours. After the cells adhered to the wall, the supernatant was discarded, and 100 μL of each group of drug-containing culture medium was added to each well, and incubated in a cell culture incubator at 37°C and 5% CO2 for 72 hours. After the incubation was completed, 10 μL of CCK-8 solution was added to each well, and the culture was continued in the incubator for 4 hours, and then the OD value of each group of cells at 450nm was detected by an ELISA instrument. The inhibition rate = (OD value of the negative control group-OD value of the drug group) / (OD value of the negative control group-OD value of the pure culture medium group) × 100%, and the OD value of each group was the average value of 5 duplicate wells. The results are shown in Table 1.

[0062] Table 1

[0063] Grouping / inhibition rate 10mg / mL 40mg / mL 80mg / mL Example 1 3.05％ 27.60％ 96.24％ Example 2 2.94％ 27.15％ 95.83％ Example 3 2.86％ 26.33％ 95.17％ Comparative Example 1 1.81％ 17.48％ 83.69％ Comparative Example 2 2.53％ 24.09％ 92.86％ Comparative Example 3 2.12％ 22.85％ 91.15％

[0064] As shown in Table 1, when the drug concentration is 10-80 mg / mL, the Chinese medicine composition of Examples 1-3 can inhibit the growth of liver cancer cells to varying degrees 72 hours after administration, among which when the drug concentration is 80 mg / mL, the inhibitory effect is the best.

[0065] Compared with Examples 1-3, the inhibition rate of Hep G2 cells in Comparative Example 1 was significantly lower, and the inhibition rate of Hep G2 cells in Comparative Examples 2-3 was slightly lower. It can be seen that the Chinese medicine composition formula designed by the present invention can inhibit the growth of liver cancer cells.

[0066] Effect Example 2

[0067] This effect example explores the anti-tumor effect of the Chinese medicine composition of Example 1 and Comparative Examples 1-3 on tumor mice. The specific process is as follows:

[0068] I. Test Materials

[0069] The mice were BALB / c nude mice, 90 in number, 5 weeks old, male, weighing 19-22 g.

[0070] II. Construction of tumor cell model

[0071] 80 BALB / c nude mice were randomly selected and injected subcutaneously in the right axilla with Hep G2 cells (5×10 6 Ten days later, a mass appeared in the right armpit of the nude mice, indicating that the tumor cell model was successfully established.

[0072] III. Grouping and Dosing

[0073] Dosage standard: The conventional oral administration dose for mice is 103.5g / kg×0.0026×50=10.53g / kg. Usually, it is also necessary to choose to increase the dose of the original medicinal materials of the Chinese medicine composition by 1 time or reduce the dose of the original medicinal materials of the Chinese medicine composition by 1 time for oral administration;

[0074] Model group: 10 rats, intragastrically administered with an equal volume of saline;

[0075] Low-dose Chinese medicine composition of Example 1 group: 10 mice, intragastrically administered with 5.27 g / kg of the Chinese medicine composition of Example 1;

[0076] Medium dose Chinese medicine composition of Example 1 group: 10 rats, intragastrically administered with 10.53 g / kg Chinese medicine composition of Example 1;

[0077] High-dose Chinese medicine composition of Example 1 group: 10 mice, intragastrically administered with 21.06 g / kg of the Chinese medicine composition of Example 1;

[0078] High-dose comparative example 1 Chinese medicine composition group: 10 rats, intragastrically administered with 21.06 g / kg comparative example 1 Chinese medicine composition;

[0079] High-dose comparative example 2 Chinese medicine composition group: 10 mice, intragastrically administered with 21.06 g / kg comparative example 2 Chinese medicine composition;

[0080] High-dose comparative example 3 Chinese medicine composition group: 10 mice, intragastrically administered with 21.06 g / kg comparative example 3 Chinese medicine composition;

[0081] Western medicine control group: 10 rats, intragastrically administered 0.03 g / kg 5-fluorouracil;

[0082] Blank control group: 10 mice, gavage with an equal volume of normal saline.

[0083] IV. Detection of tumor inhibition rate in each group

[0084] After continuous administration for 28 days, the mice in each group were weighed, and the mice were killed by cervical vertebrae dissection. The tumors and organs (lungs, liver, heart, and kidneys) of each group of mice were removed under sterile conditions and washed with physiological saline. After washing, they were blotted with filter paper, and the weight of the tumors and organs of the mice was weighed and recorded, and the tumor inhibition rate and organ index were calculated. The tumor mass of the mice was the average tumor mass of each group of mice. Tumor inhibition rate = (tumor mass of mice in the model group - tumor mass of mice in the test group) / tumor mass of mice in the model group × 100%, and the tumor mass and tumor inhibition rate of each group of mice are shown in Table 2. Organ index (mg / g) = organ mass (mg) / mouse body mass (g), and the organ index is the average organ index of each group of mice, and the results are shown in Table 3.

[0085] Table 2

[0086]

[0087]

[0088] Table 3

[0089]

[0090] As can be seen from Table 2, the medium-dose Chinese medicine composition, high-dose Chinese medicine composition and Western medicine group have a higher tumor inhibition rate on mice, which shows that the medium-dose and high-dose Chinese medicine composition can be used in the treatment of liver cancer.

[0091] As shown in Table 3, compared with the model group, the liver index, kidney index, lung index and heart index of the tumor mice in the low-dose Chinese medicine composition group, the medium-dose Chinese medicine composition group, the high-dose Chinese medicine composition group and the western medicine control group did not change significantly. The above results show that the Chinese medicine composition of the present invention has no effect on the main organ indexes of tumor mice, that is, the Chinese medicine composition of the present invention can effectively treat liver cancer without side effects.

[0092] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit them. The basic principles and main features of the present invention have been described above with specific implementation schemes. On the basis of the present invention, some modifications or replacements may be made thereto, but these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of protection claimed by the present invention.

Claims

1. A Chinese medicine composition for treating liver cancer, characterized in that: The Chinese medicine composition is composed of the following raw materials in parts by weight: 20-28 parts of kiwifruit roots, 17-22 parts of lotus roots, 12-15 parts of roxburghii, 7-10 parts of knotweed, 1-5 parts of cubeb, 5-10 parts of astragalus, 1-5 parts of quinquefolius and 1-3 parts of liquorice.

2. The Chinese medicine composition according to claim 1, characterized in that: The Chinese medicine composition is composed of the following raw materials in parts by weight: 25 parts of kiwifruit roots, 19 parts of lotus roots, 13 parts of roxburghii, 8 parts of knotweed, 3 parts of cubeb, 7 parts of astragalus, 4 parts of quinquefolius and 2 parts of liquorice.

3. The method for preparing the Chinese medicine composition according to claim 1 or 2, characterized in that: The following steps are involved: (1) Weighing and mixing the raw materials according to the weight proportions of claim 1 or 2, soaking and decocting in water, filtering after decocting to obtain a first filtrate, and retaining the drug residue; (2) soaking and decocting the residues of step (1) in water, filtering after decocting to obtain a second filtrate; (3) The first filtrate and the second filtrate are combined and concentrated to an extract with a relative density of 1.0-1.

1.

4. The method for preparing the Chinese medicine composition according to claim 3, characterized in that: The amount of water used in step (1) and step (2) is 20-30 times the total weight of the raw material.

5. The method for preparing the Chinese medicine composition according to claim 3, characterized in that: The soaking time in step (1) is 2-3 hours, and the boiling time is 3-4 hours; the soaking time in step (2) is 3-4 hours, and the boiling time is 3-4 hours.

6. The method for preparing the Chinese medicine composition according to claim 3, characterized in that: Add auxiliary materials to the traditional Chinese medicine composition to prepare corresponding dosage forms.

7. The method for preparing the Chinese medicine composition according to claim 6, characterized in that: The dosage form is an oral dosage form.

8. The method for preparing the Chinese medicine composition according to claim 7, characterized in that: The oral dosage form is oral liquid, tablet, powder, granule or capsule.

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