Device and method for collecting and monitoring organisms in water samples

Abstract

The present application is applicable to the technical field of monitoring seawater organisms, and discloses a device and method for collecting and monitoring organisms in water samples. The device includes a housing, a coarse filter component, a first connecting valve, a fine filter membrane component, a clamping component, a suction filter component, a weighing component, and a main control module. The main control module is used to control the first connecting valve, the clamping component, the suction filter component, and the weighing component. The coarse filter component is arranged outside the housing. When the clamping component clamps the fine filter membrane component, the clamping component and the fine filter membrane component enclose a filter cavity, and the coarse filter component, the first connecting valve, the filter cavity, and the suction filter component are connected in sequence through a pipeline. The weighing component has a weighing end, which is connected to the fine filter membrane component. The weighing component is used to weigh the fine filter membrane component and the target organism as a whole. The device can realize real-time collection and monitoring of organisms in water samples, improve collection efficiency, and can also realize in-situ monitoring of the growth, outbreak, and extinction of organisms in water samples.

Description

Technical Field

[0001] The present application relates to the technical field of seawater organism monitoring, and in particular to a device and method for collecting and monitoring organisms in water samples. Background Art

[0002] As marine environmental issues become increasingly severe, the demand for monitoring marine organisms, especially those that cause disasters, is growing. Monitoring marine organisms that cause disasters and diseases, and monitoring their growth, outbreaks, and extinction, is not only important for routine monitoring and emergency monitoring during outbreaks, but also reduces the human and material resources consumed by regular monitoring, enabling automated, unmanned, high-frequency collection, monitoring, and prediction of organisms.

[0003] At present, the methods of collecting and monitoring marine organisms mainly include manual sampling, plankton net sampling, drone and unmanned boat sampling, and acoustic technology monitoring.

[0004] However, manual sampling requires people to carry vehicles to the site for manual collection, which has disadvantages such as low sampling efficiency, large investment in manpower and material resources, and inability to monitor in real time. Plankton net sampling has disadvantages such as low sampling accuracy and easy damage to organisms. Unmanned collection using drones, unmanned boats and other vehicles equipped with collection tools is mostly used for planned collection and temporary and emergency collection in near-shore waters. It cannot achieve in-situ monitoring of the growth and extinction of marine organisms and other process monitoring. Acoustic technology can detect organisms in seawater through sound waves and estimate the number and type of organisms based on the sound wave reflection signals, but it has disadvantages such as high equipment cost, complex technology, and susceptibility to environmental interference. Summary of the Invention

[0005] The purpose of this application is to provide a device for collecting and monitoring organisms in water samples, which can realize real-time collection and real-time monitoring of organisms in water samples, improve collection efficiency, and realize in-situ monitoring of the growth, outbreak, and extinction of organisms in water samples.

[0006] To achieve the above objectives, the solution provided by this application is:

[0007] In a first aspect, an embodiment of the present application provides a device for collecting and monitoring organisms in water samples, comprising a housing, a coarse filtration component, a first connecting valve, a fine filtration membrane component, a clamping component, a filtration component, a weighing component, and a main control module. The housing has a cavity formed inside, and the coarse filtration component is disposed outside the housing to coarsely filter the water sample.

[0008] The first connecting valve, the fine filtration membrane assembly, the clamping assembly, the suction filtration assembly, the weighing assembly, and the main control module are all disposed in the housing; the first connecting valve, the clamping assembly, the suction filtration assembly, and the weighing assembly are all electrically connected to the main control module, and the main control module is used to control the first connecting valve, the clamping assembly, the suction filtration assembly, and the weighing assembly;

[0009] The clamping assembly includes a driving member, a first clamping member, and a second clamping member, wherein the driving member drives the first clamping member and the second clamping member to be connected to each other to clamp the fine filter membrane assembly or to be moved away from each other to release the fine filter membrane assembly;

[0010] When the clamping assembly clamps the fine filter membrane assembly, the first clamping member, the second clamping member and the fine filter membrane assembly enclose a filter cavity, and the coarse filter assembly, the first connecting valve, the filter cavity and the suction filter assembly are sequentially connected through a pipeline;

[0011] Under the action of the suction filtration component, the water sample flows through the coarse filtration component, the first connecting valve and the filtration cavity in sequence, and the fine filtration membrane component filters the water sample in the filtration cavity and intercepts the target organisms in the water sample;

[0012] The weighing component has a weighing end connected to the fine filter membrane component. When the clamping component releases the fine filter membrane component, the weighing component is at least used to weigh the fine filter membrane component and the target organism as a whole to obtain weighing data.

[0013] In a second aspect, an embodiment of the present application provides a method for collecting and monitoring organisms in water samples, which is applied to the above-mentioned device for collecting and monitoring organisms in water samples, and includes:

[0014] The main control module controls the driving member to drive the first clamping member and the second clamping member to approach each other to clamp the fine filter membrane assembly, so that the first clamping member, the second clamping member and the fine filter membrane assembly are enclosed to form a filter cavity;

[0015] The main control module controls the coarse filter component, the first connecting valve, the filter chamber and the suction filter component to be connected in sequence through pipelines;

[0016] Under the action of the suction filtration component, the water sample flows through the coarse filtration component, the first connecting valve and the filtration cavity in sequence, and the fine filtration membrane component filters the water sample in the filtration cavity and intercepts the target organisms in the water sample;

[0017] The main control module controls the driving member to drive the first clamping member and the second clamping member away from each other, so as to loosen the fine filter membrane assembly, so that the first clamping member and the second clamping member are separated from the fine filter membrane assembly;

[0018] The main control module controls the weighing component to weigh the fine filtration membrane component and the target organism as a whole to obtain weighing data.

[0019] The device for collecting and monitoring organisms in water samples provided in the embodiments of the present application has the following beneficial effects:

[0020] The device for collecting and monitoring organisms in water samples in an embodiment of the present application arranges a coarse filtration component outside the shell, and the first connecting valve, fine filtration membrane component, clamping component, filtration component, weighing component and main control module are all located inside the shell, which can reduce the impact of the water sample environment on the device, and replace manual labor with machines to achieve real-time collection and real-time monitoring of organisms.

[0021] Compared with manual sampling, the device for collecting and monitoring organisms in water samples of this embodiment uses machines instead of manual labor, can realize real-time collection and real-time monitoring of organisms, and improves collection efficiency; compared with plankton net sampling, this embodiment provides a coarse filtration component to remove large interferences in water samples and improve sampling accuracy, and provides a fine filtration membrane component to reduce damage to organisms; compared with using acoustic technology to detect organisms in water samples, it can reduce costs and simplify the method of collecting organisms, and the first connecting valve, fine filtration membrane component, clamping component, filtration component and weighing component are all arranged in the shell, which can reduce the impact of the water sample environment on the device; compared with using drones and unmanned boats to achieve unmanned sampling, it can realize in situ monitoring of the growth, outbreak, extinction and other processes of organisms in water samples. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the following briefly introduces the drawings required for use in the description of the embodiments. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on the structures shown in these drawings without any creative work.

[0023] Figure 1 Schematic diagram of the structure of the device for collecting and monitoring organisms in water samples provided in an embodiment of the present application;

[0024] Figure 2 This is a schematic structural diagram of the device for collecting and monitoring organisms in water samples provided by an embodiment of the present application, with the outer cylinder removed, from one viewing angle;

[0025] Figure 3This is a schematic structural diagram of the device for collecting and monitoring organisms in water samples provided by an embodiment of the present application, with the outer cylinder removed, viewed from another perspective;

[0026] Figure 4 This is a partial structural diagram of a device for collecting and monitoring organisms in water samples provided in an embodiment of the present application;

[0027] Figure 5 This is a structural schematic diagram of a fine filter membrane assembly in a device for collecting and monitoring organisms in water samples provided by an embodiment of the present application, in a state where the clamping assembly is released;

[0028] Figure 6 Schematic diagram of the structure of the clamping assembly in the device for collecting and monitoring organisms in water samples provided in an embodiment of the present application;

[0029] Figure 7 This is a schematic diagram of the assembly structure of the fine filter membrane component and the weighing component in the device for collecting and monitoring organisms in water samples provided in an embodiment of the present application;

[0030] Figure 8 yes Figure 7 Schematic diagram of the explosion structure;

[0031] Figure 9 Schematic diagram of the structure of the pressure member in the device for collecting and monitoring organisms in water samples provided in an embodiment of the present application;

[0032] Figure 10 Schematic diagram of the structure of the supporting member in the device for collecting and monitoring organisms in water samples provided in an embodiment of the present application;

[0033] Figure 11 This is a functional module block diagram of the automatic control circuit in the water sample organism collection and monitoring device provided in an embodiment of the present application.

[0034] Description of Figure Numbers:

[0035] 1. Device for collecting and monitoring organisms in water samples; 101. Pipeline; 1011. First pipeline; 1012. Second pipeline; 102. Air inlet pipe; 103. Air outlet pipe; 104. Sample inlet pipe; 105. Waste discharge pipe;

[0036] 10. Housing; 11. Upper cover; 12. Outer cylinder; 13. Main mounting base; 131. First main board; 132. Second main board; 133. First mounting base; 134. Second mounting base; 135. Support base; 136. Mounting plate; 14. Air outlet; 15. Waste outlet; 16. Power supply and communication interface;

[0037] 20. Coarse filter assembly; 21. Coarse filter; 30. First connecting valve; 31. Inlet channel; 32. Air inlet channel; 33. Common channel; 34. Inlet port; 35. Air port;

[0038] 40. Fine filter membrane assembly; 41. Filter membrane mounting bracket; 411. Pressing member; 4111. First pressing portion; 4112. Second pressing portion; 4113. First through hole; 4114. Connecting protrusion; 412. Supporting member; 4121. Supporting groove; 4122. Connecting groove; 4123. Second through hole; 4124. First supporting rod; 4125. Second supporting rod; 4126. Connecting portion;

[0039] 50. Clamping assembly; 51. Driving member; 511. First driving portion; 512. Second driving portion; 52. First clamping member; 53. Second clamping member; 531. Second groove;

[0040] 60. Filtration assembly; 61. First power pump; 62. Air extraction pipe; 63. Pressure sensor; 64. Air filter;

[0041] 70. Weighing assembly; 71. Weighing end; 72. Weighing sensor; 73. Acceleration sensor; 74. Connecting seat; 75. Fixed end; 80. Liquid storage assembly; 81. Liquid storage bottle; 811. Air extraction port; 82. Liquid level sensor; 90. Sample arrangement assembly; 91. Second power pump; 92. Second connecting valve; 93. First row of sample tubes; 94. Second row of sample tubes. DETAILED DESCRIPTION

[0042] The following will be combined with the drawings in the embodiments of this application to clearly and completely describe the technical solutions in the embodiments of this application. Obviously, the embodiments described are only part of the embodiments of this application, not all of the embodiments. Based on the embodiments of this application, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of this application.

[0043] It should be noted that all directional indications in the embodiments of the present application (such as up, down, left, right, front, back, etc.) are only used to explain the relative position relationship and movement status between the components in a certain specific posture. If the specific posture changes, the directional indication will also change accordingly.

[0044] It should also be noted that when an element is referred to as being "fixed to" or "disposed on" another element, it may be directly on the other element or there may be an intervening element. When an element is referred to as being "connected to" another element, it may be directly connected to the other element or indirectly connected to the other element through an intervening element.

[0045] In addition, the descriptions of "first", "second", etc. in this application are for descriptive purposes only and should not be understood as indicating or implying their relative importance or implicitly indicating the number of the technical features indicated. Therefore, the features defined as "first" or "second" may explicitly or implicitly include at least one of such features. In addition, the technical solutions between the various embodiments can be combined with each other, but this must be based on the fact that they can be implemented by ordinary technicians in this field. When the combination of technical solutions is contradictory or cannot be implemented, it should be deemed that such combination of technical solutions does not exist and is not within the scope of protection required by this application.

[0046] like Figure 1 As shown, the device 1 for collecting and monitoring organisms in water samples provided in an embodiment of the present application can be used for collecting and real-time monitoring of organisms in natural or artificial water bodies such as oceans, lakes, rivers, reservoirs, and ponds. In some application scenarios, such as application scenarios where natural seawater is used as a cooling source, such as coastal nuclear power plants, the device can automatically collect and monitor cold-source disaster-causing organisms to serve as an early warning. In aquaculture scenarios, the device can automatically collect and monitor eutrophication and pathogenic organisms in aquaculture water bodies, which helps monitor aquaculture conditions and provides data support.

[0047] like Figure 1 、 Figure 2 and Figure 3 As shown, the device 1 for collecting and monitoring organisms in water samples provided in an embodiment of the present application includes a housing 10, a coarse filter assembly 20, a first connecting valve 30, a fine filter membrane assembly 40, a clamping assembly 50, a suction filtration assembly 60 and a weighing assembly 70. The interior of the housing 10 forms a cavity, and the coarse filter assembly 20 is arranged outside the housing 10 to perform coarse filtration on the water sample; the first connecting valve 30, the fine filter membrane assembly 40, the clamping assembly 50, the suction filtration assembly 60 and the weighing assembly 70 are all arranged in the housing 10; the clamping assembly 50 is used to clamp the fine filter membrane assembly 40 or release the fine filter membrane assembly 40. When the clamping assembly 50 clamps the fine filter membrane assembly 40, the clamping assembly 50 and the fine filter membrane assembly 40 enclose a filter cavity (not shown in the figure), and the coarse filter assembly 20, the first connecting valve 30, the filter cavity and the suction filtration assembly 60 are sequentially connected through a pipeline 101;

[0048] Under the action of the suction filtration component 60, the water sample flows through the coarse filtration component 20, the first connecting valve 30 and the filter cavity in sequence, and the fine filter membrane component 40 filters the water sample in the filter cavity and intercepts the target organisms in the water sample; the weighing component 70 has a weighing end 71, which is connected to the fine filter membrane component 40. When the clamping component 50 releases the fine filter membrane component 40, the weighing component 70 is at least used to weigh the fine filter membrane component 40 and the target organism as a whole, and obtain weighing data to monitor the collection amount of the target organism.

[0049] In this embodiment, by arranging the coarse filtration component 20 outside the shell 10, the first connecting valve 30, the fine filtration membrane component 40, the clamping component 50, the filtration component 60 and the weighing component 70 are all located inside the shell 10, which can reduce the impact of the water sample environment on the device, and replace manual labor with machines to achieve real-time collection and real-time monitoring of organisms. When using this device, when it is necessary to collect organisms in water samples, the interior of the shell 10 can be assumed to be in a vacuum state, and the clamping component 50 clamps the fine filter membrane component 40, so that the clamping component 50 and the fine filter membrane component 40 are enclosed to form a filter chamber, so that the coarse filter component 20, the first connecting valve 30, the filter chamber and the suction filter component 60 are connected in sequence. In this way, the water sample enters the device from the coarse filter component 20 under the suction of the suction filter component 60. The coarse filter component 20 can perform coarse filtration on the water sample, for example, intercept large interferences in the water sample, such as intercepting leaves, sand, plastic particles, etc. The coarsely filtered water sample enters the filter chamber under the transportation of the first connecting valve 30, and is filtered twice by the fine filter membrane component 40. At this time, the fine filter membrane component 40 can intercept and collect the target organisms required, simplifying the method of collecting organisms, and after coarse filtration, the collection accuracy of the target organisms can be improved to a certain extent.

[0050] After collection is complete, the filtration assembly 60 stops operating, and the clamping assembly 50 releases the fine filter membrane assembly 40. At this point, the fine filter membrane assembly 40 and the target organism act as a whole, exerting a force on the weighing end 71 of the weighing assembly 70. The weighing assembly 70 can weigh the fine filter membrane assembly 40 and the target organism as a whole to obtain data, such as weight or pressure data. It should be understood that before collection, and when the clamping assembly 50 releases the fine filter membrane assembly 40, the weighing assembly 70 can obtain the force exerted by the fine filter membrane assembly 40 on the weighing end 71, that is, the weighing assembly 70 can also weigh the fine filter membrane assembly 40.

[0051] Wherein, target organism can be understood as organism to be monitored, can also be understood as the organism that user wants to monitor, it should be noted that, in conjunction with correlation technology, when weighing, multiple weighing data can be obtained in short time, then the mean value of multiple weighing data is obtained, using the mean value of weighing data as the data required for this collection, to improve weighing accuracy.The present embodiment can realize real-time in-situ collection of the organism in water sample, and real-time in-situ monitoring is carried out to organism, by completing multiple collections, obtain multiple weighing data (average weighing data obtained when being understood as completing single collection), multiple weighing data are compared, analyze, such as with time as abscissa, weighing data as ordinate, plot curve, observe whether weighing data increases sharply in short time, drops sharply or constant value is constant, judge whether target organism increases or reduces in short time, can monitor the growth of target organism in water sample to a certain extent, outburst, the processes such as extinction.

[0052] Compared with manual sampling, the device 1 for collecting and monitoring organisms in water samples of this embodiment uses machines instead of manual labor, which can realize real-time collection and real-time monitoring of organisms and improve collection efficiency; compared with plankton net sampling, this embodiment is provided with a coarse filter component 20, which can remove large interferences in the water sample and improve sampling accuracy, and is provided with a fine filter membrane component 40, which can reduce damage to organisms; compared with using acoustic technology to detect organisms in water samples, it can reduce costs and simplify the method of collecting organisms, and the first connecting valve 30, fine filter membrane component 40, clamping component 50, filtration component 60 and weighing component 70 are all arranged in the shell 10, which can reduce the impact of the water sample environment on the device; compared with using drones or unmanned boats to achieve unmanned sampling, it can realize in-situ monitoring of the growth, outbreak, extinction and other processes of organisms in water samples.

[0053] Combine Figure 1 and Figure 2 In some embodiments, the device for collecting and monitoring organisms in water samples 1 further includes a main control module (not shown). The main control module is disposed in the housing 10. The first connecting valve 30, the clamping assembly 50, the filtration assembly 60, and the weighing assembly 70 are all electrically connected to the main control module. The main control module is used to control the first connecting valve 30, the clamping assembly 50, the filtration assembly 60, and the weighing assembly 70. The weighing assembly 70 is also used to send weighing data to the main control module.

[0054] In this embodiment, the provision of a main control module enables automated control of the first connecting valve 30, the clamping assembly 50, the filtration assembly 60, and the weighing assembly 70, thereby improving monitoring efficiency. In some embodiments, the main control module includes a controller that can be computer-programmed, so that the method for controlling the automated operation of the device can be burned into the controller in the form of program firmware, thereby achieving automated control of the first connecting valve 30, the clamping assembly 50, the filtration assembly 60, and the weighing assembly 70, thereby enabling the device to stably monitor the amount of organisms collected in seawater over a long period of time.

[0055] like Figure 2 、 Figure 3 and Figure 5 As shown, in some embodiments, the clamping assembly 50 includes a driving member 51, a first clamping member 52 and a second clamping member 53. The driving member 51 drives the first clamping member 52 and the second clamping member 53 to drive the first clamping member 52 and the second clamping member 53 to move closer to each other to clamp the fine filter membrane assembly 40 or move away from each other to loosen the fine filter membrane assembly 40; when the fine filter membrane assembly 40 is clamped, the first clamping member 52, the second clamping member 53 and the fine filter membrane assembly 40 are enclosed to form a filter cavity.

[0056] In this embodiment, when it is necessary to collect organisms from a water sample, the first and second clamping members 52, 53 of the clamping assembly 50 are moved closer together under the action of the driver 51 to clamp the fine filter membrane assembly 40. At this point, the first and second clamping members 52, 53, and fine filter membrane assembly 40 enclose a filtration chamber, thereby sequentially connecting the coarse filter assembly 20, the first connecting valve 30, the filtration chamber, and the suction filtration assembly 60. After collection is complete, the suction filtration assembly 60 stops operating, and the first and second clamping members 52, 53 are moved away from each other under the action of the driver 51 to release the fine filter membrane assembly 40.

[0057] like Figure 2 、 Figure 5 and Figure 6 As shown, in some embodiments, the driving member 51 includes a first driving portion 511 and a second driving portion 512 disposed opposite each other. The first driving portion 511 is driven to connect to the first clamping member 52, and the second driving portion 512 is driven to connect to the second clamping member 53. The driving member 51 drives the first driving portion 511 and the second driving portion 512 to move toward or away from each other, thereby driving the first clamping member 52 and the second clamping member 53 to move toward or away from each other, thereby moving the first clamping member 52 and the second clamping member 53 toward each other to clamp the fine filter membrane assembly 40, or away from each other to release the fine filter membrane assembly 40. In some embodiments, the driving member 51 is electrically connected to a main control module, which controls the driving member 51 to drive the first driving portion 511 and the second driving portion 512 toward or away from each other. Exemplarily, the driving member 51 is an electric clamp, and the first driving portion 511 and the second driving portion 512 are both clamps.

[0058] like Figure 1 、 Figure 2 and Figure 3As shown, in some embodiments, the housing 10 includes an upper cover 11 and an outer cylinder 12. The upper cover 11 is connected to the top of the outer cylinder 12 and covers the outer cylinder 12. The upper cover 11 and the outer cylinder 12 enclose a cavity, simplifying the structure of the housing 10. The first connecting valve 30, the fine filter membrane assembly 40, the clamping assembly 50, the suction filtration assembly 60, and the weighing assembly 70 are all accommodated in the outer cylinder 12. In some embodiments, the housing 10 also includes a main mounting seat 13 installed in the outer cylinder 12. The first connecting valve 30, the fine filter membrane assembly 40, the clamping assembly 50, the suction filtration assembly 60, and the weighing assembly 70 are all mounted on the main mounting seat 13 to improve installation stability. In some embodiments, the main mounting base 13 includes a first main plate 131 and a second main plate 132 located at the bottom of the first main plate 131. The first and second main plates 131 and 132 are connected at an angle and enclose a space for accommodating the first connecting valve 30, the fine filter membrane assembly 40, the clamping assembly 50, the filtration assembly 60, and the weighing assembly 70. In some embodiments, a first mounting seat 133 and a second mounting seat 134 are provided on the inner side of the main mounting base 13. The first connecting valve 30 and the clamping assembly 50 are respectively assembled on the first mounting seat 133 and the second mounting seat 134.

[0059] like Figure 2 and Figure 3 As shown, in some embodiments, the coarse filter assembly 20 includes a coarse filter 21 and a coarse filter screen, filter cotton, etc. arranged in the coarse filter 21, which are used to filter large interferences such as sand and gravel in the water sample. When the device is working, the coarse filter 21 is placed below the water surface, and the water sample enters the device after passing through the coarse filter 21. In specific applications, the material of the coarse filter 21 is preferably 316L stainless steel, titanium alloy, etc., so that the coarse filter 21 is not easily corroded by substances such as acids, alkalis, and salts in the water. The mesh size of the coarse filter screen in the coarse filter 21 is preferably 20 to 200 meshes, which can effectively intercept large interferences in the water, such as leaves, sand, small and larger aquatic organisms, etc. In practice, the mesh size of the coarse filter screen can be determined according to the water quality of the sea area where the device is placed.

[0060] like Figure 2 and Figure 5 As shown, in some embodiments, the fine filter membrane assembly 40 includes a filter membrane fixing frame 41 and a fine filter membrane (not shown in the figure), and the fine filter membrane is installed on the filter membrane fixing frame 41 to intercept the target organisms in the water sample; the first clamping member 52 and the second clamping member 53 clamp or release the filter membrane fixing frame 41, and when the filter membrane fixing frame 41 is clamped, the first clamping member 52, the second clamping member 53 and the filter membrane fixing frame 41 are enclosed to form a filter cavity; the weighing end 71 is connected to the filter membrane fixing frame 41.

[0061] In this embodiment, the fine filter membrane is fixedly mounted on the filter membrane holder 41, which can carry the fine filter membrane. The first clamping member 52 and the second clamping member 53 cooperate to clamp the filter membrane holder 41, so that the first clamping member 52, the second clamping member 53 and the filter membrane holder 41 enclose and form a sealed filter cavity. The fine filter membrane is mounted on the filter membrane holder 41 to filter the water sample entering the filter cavity to intercept the target organism. After the collection is completed, the first clamping member 52 and the second clamping member 53 cooperate to loosen the filter membrane holder 41. At this time, the filter membrane holder 41, the fine filter membrane and the target organism trapped on the fine filter membrane jointly apply a force to the weighing end 71, and compress the weighing end 71 to cause the weighing end 71 to deform. The weighing assembly 70 can sense the force applied to the weighing end 71.

[0062] In some embodiments, the fine filter membrane is preferably made of an organic microporous filter material such as polyvinylidene fluoride, which exhibits excellent hydrophilicity. The pore size of the fine filter membrane is preferably 1 to 100 μm. In specific applications, the pore size of the fine filter membrane is selected based on the particle size of the target organism. This allows organisms larger than the pore size to be retained on the filter screen, while organisms, metal ions, and molecules smaller than the pore size can pass through. In this way, the target organisms to be monitored in the water sample can be retained on the fine filter membrane.

[0063] like Figure 5 and Figure 6 As shown, in some embodiments, a first sealing member (not shown) is provided on the side of the first clamping member 52 facing the filter membrane holder 41, and a second sealing member (not shown) is provided on the side of the second clamping member 53 facing the filter membrane holder 41. When the first clamping member 52 and the second clamping member 53 cooperate to clamp the filter membrane holder 41, the first sealing member seals one side of the first clamping member 52 and the filter membrane holder 41, and the second sealing member seals the other side of the second clamping member 53 and the filter membrane holder, thereby improving the sealing performance of the filter cavity. Exemplarily, the first sealing member and the second sealing member are both sealing gaskets. In some embodiments, the first clamping member 52 is provided with a first groove (not shown), the first groove faces the second clamping member 53, the second clamping member 53 is provided with a second groove 531, the second groove 531 faces the first clamping member 52, and when the clamping assembly 50 clamps the fine filter membrane assembly 40, the first groove and the second groove 531 are respectively located on the two sides of the filter membrane fixing frame 41. The setting of the first groove and the second groove 531 can increase the volume of the filter chamber.

[0064] like Figure 2 and Figure 7As shown, in some embodiments, the filter membrane fixing frame 41 includes a pressing member 411 and a supporting member 412. In the movement direction of the first clamping member 52, the fine filter membrane is arranged between the pressing member 411 and the supporting member 412. The pressing member 411 and the supporting member 412 are respectively pressed on both sides of the fine filter membrane to press and fix the fine filter membrane; the weighing end 71 is connected to the supporting member 412.

[0065] In this embodiment, the supporting member 412 is located below the pressing member 411. The supporting member 412 can support the fine filter membrane. The pressing member 411 and the supporting member 412 jointly press the fine filter membrane. When the filter membrane holder 41 is clamped, the first clamping member 52 and the second clamping member 53 cooperate to press the pressing member 411 and the supporting member 412 against the upper and lower sides of the fine filter membrane, respectively. Thus, the first clamping member 52, the second clamping member 53, the pressing member 411, and the supporting member 412 can enclose and form a sealed filter cavity, thereby improving the sealing of the filter cavity. The weighing end 71 is connected to the supporting member 412, so that during weighing, the filter membrane holder 41, the fine filter membrane, and the target organism jointly apply a force to the weighing end 71.

[0066] like Figure 8 、 Figure 9 and Figure 10 As shown, in some embodiments, the pressing member 411 includes a first pressing portion 4111 and a second pressing portion 4112, which are connected in sequence along the same axis. The pressing member 411 is provided with a first through-hole 4113 for the passage of the water sample, which extends through the first and second pressing portions 4111, 4112. The outer peripheral wall of the second pressing portion 4112 is provided with a connecting protrusion 4114. Simultaneously, the supporting member 412 is provided with a supporting groove 4121, the inner peripheral wall of which is provided with a connecting groove 4122. The second pressing portion 4112 extends into the supporting groove 4121, and the connecting protrusion 4114 engages with the connecting groove 4122, pressing the peripheral portion of the fine filter membrane against the connecting groove 4122. This simplifies the connection between the pressing member 411 and the supporting member 412, while improving the assembly stability of the fine filter membrane.

[0067] like Figure 8 and Figure 10 As shown, in some embodiments, the bottom of the supporting groove 4121 is provided with a second through-hole 4123 for the passage of the water sample. The centerline of the second through-hole 4123 coincides with the centerline of the first through-hole 4113. A first supporting rod 4124 and a second supporting rod 4125 are disposed within the first through-hole 4113. The first supporting rod 4124 and the second supporting rod 4125 are connected at an angle to support the fine filter membrane. A connecting portion 4126 extends from the outer wall of the supporting member 412. The connecting portion 4126 is connected to the weighing end 71, thereby connecting the supporting member 412 to the weighing end 71.

[0068] like Figures 1 to 3As shown, combined with Figure 5 In some embodiments, the weighing assembly 70 includes a weighing sensor 72, an acceleration sensor 73 and a connecting seat 74. The weighing sensor 72 is installed in the housing 10 through the connecting seat 74. The weighing end 71 is provided on the weighing sensor 72. The weighing sensor 72 is at least used to monitor the pressure value applied by the fine filter membrane assembly 40 and the target organism to the weighing end 71; the acceleration sensor 73 is used to monitor the acceleration value in the same direction as the force direction of the weighing end 71.

[0069] In this embodiment, the fixed end 75 of the weighing sensor 72 is mounted on the connecting seat 74, and the weighing end 71 of the weighing sensor 72 is fixed to the fine filter membrane assembly 40. When the device is collecting a target organism, the clamping assembly 50 clamps the fine filter membrane assembly 40, and the fine filter membrane assembly 40 is connected to the first connecting valve 30 and the suction filter assembly 60, respectively, to form a filtration collection system. When the device is in a weighing state, the clamping assembly 50 does not clamp the fine filter membrane assembly 40. At this time, the fine filter membrane assembly 40 is disconnected from the first connecting valve 30 and the suction filter assembly 60, respectively. The fine filter membrane assembly 40 and the weighing assembly 70 form a dynamic weighing system, which facilitates the weighing assembly 70 to weigh the fine filter membrane assembly 40 and the target organism as a whole. Among them, the weighing sensor 72 and the acceleration sensor 73 are both existing sensors. For example, the weighing sensor 72 is a load cell.

[0070] As will be appreciated, the present device is primarily mounted on a floating platform, such as a buoy. Water waves cause the floating platform to move, and the device and its internal weighing sensor 72 will follow the platform's movement. Therefore, an acceleration sensor 73 is provided within the housing 10 to effectively mitigate the impact of the weighing sensor 72's movement on the weighing results, thereby improving monitoring accuracy. The weighing sensor 72 senses the pressure exerted by the fine filter membrane assembly 40 and the target organism at the weighing end 71, converting it into a digital quantity that can be read and calculated by a computer or other device. The acceleration sensor 73 has a sensing axis aligned with the pressure-sensitive direction of the weighing end 71. The acceleration sensor 73 converts analog acceleration in the sensing axis direction into a digital quantity. Thus, according to Newton's second law, m = F / a, the overall mass of the fine filter membrane assembly 40 and the target organism can be calculated, improving monitoring efficiency. Similarly, the mass of the fine filter membrane assembly 40 before sampling can also be determined. For example, by pre-obtaining the pressure exerted by the fine filter membrane assembly 40 at the weighing end 71 and the corresponding acceleration value, the mass of the sampled target organism can be determined.

[0071] Furthermore, for each weighing after collection, multiple pressure values ​​and acceleration values ​​can be obtained within a short period of time, such as 60 seconds, to calculate multiple overall mass values ​​of the fine filter membrane assembly 40 and the target organism. An average mass value is then obtained, and the mass value of the fine filter membrane assembly 40 before collection is subtracted from the average mass value to obtain the mass value of the collected target organism. In this way, after multiple collections are performed and multiple average mass values ​​of the target organism are obtained, a curve is plotted with time as the horizontal axis and mass as the vertical axis to observe whether the mass value of the target organism increases or decreases sharply within a short period of time, or remains constant. This can determine whether the target organism increases or decreases within a short period of time, and can, to a certain extent, monitor the growth, outbreak, and extinction of the target organism in the water sample. In some embodiments, the weighing sensor 72 transmits the acquired pressure values ​​to the main control module, and the acceleration sensor 73 transmits the acquired acceleration values ​​to the main control module, which then transmits them to a computer or other device for calculation.

[0072] like Figure 2 and Figure 3 As shown, in some embodiments, the first connecting valve 30 is a three-way valve, and the first connecting valve 30 is provided with an injection channel 31, an air inlet channel 32, and a common channel 33. When one of the injection channel 31 and the air inlet channel 32 is open, the other is closed. It can be understood that during injection, the injection channel 31 and the common channel 33 are connected, and the air inlet channel 32 is closed. During filtration, the air inlet channel 32 and the common channel 33 are connected, and the injection channel 31 is closed. The injection channel 31 is connected to the coarse filter assembly 20 through a pipeline 101. The air inlet channel 32 is connected to an air inlet pipe 102. The end of the air inlet pipe 102 away from the air inlet channel 32 extends outside the housing 10. Air can enter the filter cavity from the air inlet pipe 102. The common channel 33 is connected to the filter cavity through the pipeline 101. When the sampling channel 31 is opened, the suction filtration component 60 allows the water sample to flow through the coarse filtration component 20 and the first connecting valve 30 in sequence, enter the filter cavity for filtration, and then extract the water sample from the filter cavity; when the air inlet channel 32 is opened, the suction filtration component 60 allows air to enter the filter cavity from the air inlet pipe 102 through the first connecting valve 30, so that the water sample passes through the fine filter membrane component 40.

[0073] In this embodiment, during sampling, the sampling channel 31 and the common channel 33 in the three-way valve are connected, and the filtration assembly 60 draws water sample, which enters the device from the coarse filtration assembly 20 and then passes through the first connecting valve 30 into the filtration chamber. The sampling channel 31 is then closed, and the air intake channel 32 and the common channel 33 are connected. At the same time, the filtration assembly 60 draws air, so that the air enters the first connecting valve 30 from the air intake pipe 102 and then enters the filtration chamber, causing the water sample to pass through the fine filtration membrane assembly 40. In some embodiments, the first connecting valve 30 has an sampling end 34 and an air end 35. The main control module controls the switching between the sampling end 34 and the air end 35. For example, the main control module controls the first connecting valve 30 to open the sampling end 34 and close the air end 35. At this time, the sampling channel 31 and the common channel 33 are connected. After a period of sampling, the main control module controls the first connecting valve 30 to switch to the air end 35. At this time, the sampling channel 32 and the common channel 33 are connected.

[0074] like Figure 2 、 Figure 3 and Figure 4 As shown, in some embodiments, the filtration component 60 includes a first power pump 61, an air extraction pipe 62 and a pressure sensor 63. The water sample organism collection and monitoring device 1 also includes a liquid storage component 80. The liquid storage component 80 has a liquid storage cavity (not shown in the figure). The liquid storage cavity is connected to the filtration cavity through a pipeline 101. One end of the air extraction pipe 62 is connected to the liquid storage cavity, and the other end of the air extraction pipe 62 is connected to the first power pump 61. The pressure sensor 63 is provided on the air extraction pipe 62 to monitor the pressure on the air extraction pipe 62.

[0075] In this embodiment, the first power pump 61 provides power during the sampling process. The first power pump 61 is turned on, and the sampling end 34 of the first connecting valve 30 is opened, and the air end 35 of the first connecting valve 30 is closed. At this time, the water sample flows through the coarse filter component 20 and the first connecting valve 30 in sequence, and then enters the filter chamber. After a period of sampling, the first connecting valve 30 closes the sampling end 34 and opens the air end 35. The air flows through the first connecting valve 30 into the filter chamber in turn, and is then extracted from the exhaust pipe 62 by the first power pump 61. During this process, the first power pump 61 can pump air to form a negative pressure in the pipeline 101 at the rear section of the fine filter membrane assembly 40 (the pipeline 101 connecting the filter chamber and the liquid storage chamber), thereby prompting the water sample to pass through the fine filter membrane assembly 40. Specifically in this embodiment, the water sample will pass through the fine filter membrane and flow into the liquid storage chamber, and then the target organism will be retained on the fine filter membrane. After the filtration is completed, the first power pump 61 is turned off, and the clamping assembly 50 is controlled to loosen the fine filter membrane assembly 40. The weighing assembly 70 weighs the fine filter membrane assembly 40 and the target organism retained on the fine filter membrane assembly 40 as a whole to obtain weighing data. Exemplarily, the first power pump 61 is a filtration pump, and the liquid storage assembly 80 includes a liquid storage bottle 81 having a liquid storage cavity. The liquid storage bottle 81 is fixedly mounted on the main mounting seat 13. Specifically, the liquid storage bottle 81 is mounted on the second main board 132 through the support seat 135.

[0076] In this embodiment, a pressure sensor 63 is provided on the air extraction pipe 62. The pressure sensor 63 can read the pressure within the liquid storage chamber in real time. The pressure sensor 63 is used to monitor the filtration pressure in real time during filtration. By observing any sudden changes in the filtration pressure, it can be determined whether the fine filter membrane assembly 40 is clogged, and thus whether the fine filter membrane in the fine filter membrane assembly 40 needs to be replaced. Furthermore, it can be understood that, according to the aforementioned formula m = F / a for calculating the combined mass of the fine filter membrane assembly 40 and the target organism, when a approaches 0, m cannot be calculated. This is the case in extreme operating conditions such as when the device is in a horizontal roll, i.e., when the sensing axis of the acceleration sensor 73 is completely perpendicular to the direction of gravity. Therefore, the filtration pressure and filtration duration can be combined to assist in determining the mass of the organism on the fine filter membrane. For example, by pre-establishing a relationship between filtration pressure, filtration duration, and the mass of the organism on the fine filter membrane, the filtration pressure and filtration duration can be used to assist in mass calculation, thereby improving monitoring accuracy.

[0077] like Figure 4 As shown, in some embodiments, the first power pump 61 and the pressure sensor 63 are both electrically connected to the main control module. The main control module obtains the value of the pressure sensor 63 to control the first power pump 61 to operate within a preset pressure range. The preset pressure range can be understood as a pressure value range set in actual application.

[0078] like Figures 3 to 6As shown, in some embodiments, the pipeline 101 includes a first pipeline 1011 and a second pipeline 1012. The first pipeline 1011 is arranged between the first connecting valve 30 and the first clamping member 52. One end of the first pipeline 1011 is connected to the first connecting valve 30, and the other end of the first pipeline 1011 passes through the first clamping member 52, so that the first clamping member 52 can move on the first pipeline 1011. The second pipeline 1012 is arranged between the second clamping member 53 and the liquid storage assembly 80. One end of the second pipeline 1012 is connected to the liquid storage chamber (specifically, connected to the liquid inlet of the liquid storage bottle), and the other end of the second pipeline 1012 passes through the second clamping member 53, so that the second clamping member 53 can move on the second pipeline 1012. During specific collection, the clamping assembly 50 clamps the fine filter membrane assembly 40, the first connecting valve 30 is connected to the filter chamber through the first pipeline 1011, the liquid storage bottle is connected to the filter chamber through the second pipeline 1012, and the liquid storage bottle is also connected to the first power pump 61 through the exhaust pipe 62.

[0079] like Figure 1 and Figure 4 As shown, in some embodiments, the first power pump 61 has a first inlet (not shown) and a first outlet (not shown), and the housing 10 is provided with an air outlet 14. An air outlet pipe 103 is mounted on the air outlet 14. One end of the air outlet pipe 103 is connected to the first outlet, and the other end of the air outlet pipe 103 extends outside the housing 10. In this embodiment, air entering the liquid storage chamber can flow through the air extraction pipe 62 and the air outlet pipe 103 in sequence under the suction action of the first power pump 61 and be discharged outside the device.

[0080] like Figure 1 and Figure 4 As shown, in some embodiments, the liquid storage chamber is provided with an air extraction port 811, and the end of the air extraction pipe 62 remote from the first power pump 61 is connected to the air extraction port 811. In this embodiment, air entering the liquid storage chamber can flow out of the air extraction port 811 under the suction action of the first power pump 61, and then flow through the air extraction pipe 62 and the air outlet pipe 103, and then be discharged outside the device. In some embodiments, the air extraction port 811 is located at the upper portion of the liquid storage bottle 81.

[0081] like Figure 1 、 Figure 3 and Figure 4 As shown, in some embodiments, the filtration assembly 60 further includes an air filter 64, which is disposed on the air inlet pipe 102 and communicates with the air inlet passage 32 via the air inlet pipe 102. In this embodiment, the air filter 64 is built into the housing 10. Filter cotton, activated carbon, and the like are disposed within the air filter 64, effectively preventing impurities such as dust and grit carried by the air during the filtration process from entering the device and adversely affecting the monitoring data, thereby improving monitoring accuracy.

[0082] like Figure 1 、 Figure 2 and Figure 4 As shown, in some embodiments, the housing 10 is provided with an air inlet (not shown), to which an air inlet pipe 102 is mounted, and the air inlet pipe 102 is rationally arranged. The housing 10 is also provided with a sample inlet (not shown), to which a sample inlet pipe 104 is mounted. One end of the sample inlet pipe 104 is connected to the coarse filter assembly 20, and the other end of the sample inlet pipe 104 is connected to the sample inlet channel 31. In this embodiment, the water sample enters the coarse filter assembly 20, undergoes primary filtration, and then flows into the first connecting valve 30 through the sample inlet pipe 104.

[0083] like Figure 1 、 Figure 2 and Figure 4 As shown, in some embodiments, the shell 10 is further provided with a waste outlet 15, a waste outlet pipe 105 is installed on the waste outlet 15, and the liquid storage chamber is provided with a sample discharge port (not shown); the water sample collection and monitoring device 1 also includes a sample discharge component 90, and the sample discharge component 90 includes a second power pump 91 and a second connecting valve 92. One end of the second power pump 91 is connected to the sample discharge port through the second connecting valve 92, and the other end of the second power pump 91 is connected to one end of the waste outlet pipe 105, and the other end of the waste outlet pipe 105 extends out of the shell 10; the second power pump 91 and the second connecting valve 92 are opened during sample discharge, and the second power pump 91 provides power during sample discharge.

[0084] In this embodiment, a waste outlet 15 is provided on the housing 10, a sample outlet is provided on the liquid storage chamber, and a sample outlet assembly 90 is provided, so that the water sample in the liquid storage chamber can be discharged when needed. Specifically, when discharging waste, the second power pump 91 and the second connecting valve 92 are turned on, and the water sample in the liquid storage chamber flows from the sample outlet through the second connecting valve 92 and the second power pump 91 in sequence, and then is discharged from the housing 10 through the waste pipe 105. In some embodiments, the sample inlet, the air inlet, the air outlet 14 and the waste outlet 15 are all provided on the upper cover 11, and the upper cover 11 is also provided with a power supply communication interface 16 for connecting a power source to power the device. Exemplarily, the sample outlet is provided at the bottom of the liquid storage bottle 81.

[0085] like Figure 1 、 Figure 2 and Figure 4As shown, in some embodiments, the second power pump 91 and the second connecting valve 92 are both electrically connected to the main control module. The main control module can control the second power pump 91 to be opened or closed, and can also control the second connecting valve 92 to be opened or closed. The second power pump 91 has a second inlet (not shown) and a second outlet (not shown). The discharge port is connected to the second inlet through the second connecting valve 92. One end of the waste pipe 105 is connected to the second outlet, and the other end of the waste pipe 105 extends out of the shell 10. During discharge, the main control module controls the second connecting valve 92 and the second power pump 91 to be opened. In this embodiment, after the weighing is completed, the air end 35 of the first connecting valve 30 can be opened, the sample inlet end 34 of the first connecting valve 30 can be closed, and the second connecting valve 92 and the second power pump 91 can be opened at the same time. In this case, air flows from the air inlet pipe 102 through the air filter 64 into the housing 10, and performs gas-liquid exchange with the water sample in the liquid storage bottle, so that the water sample in the liquid storage bottle can flow through the sample discharge port, the second connecting valve 92, the second power pump 91, the waste pipe 105, and finally out of the housing 10, thereby emptying the water sample in the liquid storage bottle and preparing for the next weighing. For example, the second power pump 91 is a waste pump and the second connecting valve 92 is a two-way valve.

[0086] Furthermore, the sample discharge assembly 90 further includes a first sample discharge tube 93 and a second sample discharge tube 94. One end of the first sample discharge tube 93 is connected to the sample discharge port, and the other end of the first sample discharge tube 93 is connected to the inlet of the second connecting valve 92. One end of the second sample discharge tube 94 is connected to the outlet of the second connecting valve 92, and the other end of the second sample discharge tube 94 is connected to the second inlet, thereby respectively connecting the second connecting valve 92 to the liquid storage bottle 81 and the second power pump 91. In some embodiments, a mounting plate 136 perpendicular to the second main body 132 is mounted on the second main body 132. The mounting plate 136 is located between the first main body 131 and the liquid storage bottle 81. The first power pump 61, the second power pump 91, and the second connecting valve 92 are all mounted on the mounting plate 136, so as to rationally utilize the space between the first main body 131 and the liquid storage bottle 81.

[0087] like Figure 2 、 Figure 3 and Figure 4 As shown, in some embodiments, the liquid storage assembly 80 further includes a liquid level sensor 82, which is disposed on the side wall of the liquid storage chamber and is used to monitor the amount of water sample in the liquid storage chamber; the liquid level sensor 82 is also used to sense that when the liquid level of the water sample in the liquid storage chamber rises to a set height value, it triggers the second power pump 91 to turn on and the first power pump 61 to turn off, thereby discharging the water sample in the liquid storage chamber. In this embodiment, the liquid level sensor 82 is provided to monitor whether there is too much liquid in the liquid storage chamber, thereby deciding whether to turn on the second power pump 91 and turn off the first power pump 61. In some embodiments, the liquid level sensor 82 is disposed on the outer wall of the liquid storage bottle 81 and is located below the air extraction port 811.

[0088] In some embodiments, two liquid level sensors 82 are provided, and both liquid level sensors 82 are electrically connected to the main control module. The two liquid level sensors 82 are symmetrically arranged with the liquid storage chamber as the center. When the two liquid level sensors 82 simultaneously sense the water sample in the liquid storage chamber, they send a sensing signal to the main control module to trigger the opening of the second power pump 91 to discharge waste, close the first power pump 61, and stop pumping or filtering.

[0089] Combine Figure 1 and Figure 2 The present application also provides a method for collecting and monitoring organisms in water samples, which is applied to the above-mentioned device for collecting and monitoring organisms in water samples 1, and includes:

[0090] The main control module controls the driving member 51 to drive the first clamping member 52 and the second clamping member 53 to move closer to each other to clamp the fine filter membrane assembly 40, so that the first clamping member 52, the second clamping member 53 and the fine filter membrane assembly 40 are enclosed to form a filter cavity;

[0091] The main control module controls the coarse filter assembly 20, the first connecting valve 30, the filter chamber and the suction filter assembly 60 to be connected in sequence through the pipeline 101;

[0092] Under the action of the suction filtration assembly 60, the water sample flows through the coarse filtration assembly 20, the first connecting valve 30 and the filter cavity in sequence. The fine filtration membrane assembly 40 filters the water sample in the filter cavity and intercepts the target organisms in the water sample;

[0093] The main control module controls the driving member 51 to drive the first clamping member 52 and the second clamping member 53 away from each other, so as to loosen the fine filter membrane assembly 40, so that the first clamping member 52 and the second clamping member 53 are separated from the fine filter membrane assembly;

[0094] The main control module controls the weighing assembly 70 to weigh the fine filtration membrane assembly 40 and the target organism as a whole to obtain weighing data.

[0095] It can be understood that the method for collecting and monitoring organisms in water samples of this embodiment uses the above-mentioned device 1 for collecting and monitoring organisms in water samples, which can realize real-time collection and real-time monitoring of organisms in water samples, improve collection efficiency, and realize in-situ monitoring of the growth, outbreak, and extinction of organisms in water samples.

[0096] Combine Figures 1 to 5 In some embodiments, in combination with related technologies, the controller of the main control module is equipped with an automatic control circuit to enable the device to operate automatically. Specifically, Figure 11 As shown, the automation control circuit includes a main control circuit, a pressure sensing circuit, a liquid level sensing circuit, a weighing sensing circuit, an acceleration sensing circuit, a data transmission circuit, a first drive circuit, a second drive circuit, a third drive circuit, a fourth drive circuit, a fifth drive circuit and a power management circuit.

[0097] Among them, the pressure sensing circuit is used to transmit the pressure signal of the pressure sensor 63 to the main control circuit, the liquid level sensing circuit is used to transmit the liquid level signal of the liquid level sensor 82 to the main control circuit, the weighing sensing circuit is used to transmit the pressure value signal of the weighing sensor 72 to the main control circuit, the acceleration sensing circuit is used to transmit the acceleration signal of the acceleration sensor 73 to the main control circuit, the data transmission circuit includes a transmission antenna and a wireless data transceiver to establish communication between the controller and a computer and other equipment, and the first drive circuit is used to control the first dynamic control circuit under the control of the main control circuit during filtration or pumping. The power pump 61 is turned on, the second drive circuit is used to control the second power pump 91 to turn on when discharging waste under the control of the main control circuit, the fourth drive circuit is used to control the second connecting valve 92 to turn on when discharging waste under the control of the main control circuit, the third drive circuit is used to control the air end 35 or the injection end 34 of the first connecting valve 30 to turn on under the control of the main control circuit, and the fifth drive circuit is used to control the drive member 51 under the control of the main control circuit to control the first drive part 511 and the second drive part 512 to move toward or away from each other. The power management circuit includes an overcurrent insurance circuit, a rectifier and voltage stabilization circuit, etc.

[0098] Thus, when using the device for automated collection, assuming that no liquid remains inside the device, in this case, the method for automated collection using the device includes:

[0099] S1. The main control circuit controls the fifth drive circuit to operate the clamping assembly 50 in a clamped state. At this time, the first clamping member 52 and the second clamping member 53 clamp the fine filter membrane assembly 40, the first connecting valve 30, the filter chamber, and the liquid storage chamber are connected, and the fine filter membrane assembly 40 is connected to the weighing end 71 of the weighing assembly 70.

[0100] S2: The main control circuit controls the third drive circuit to switch the working state of the first connecting valve 30 to connect the injection end 34 with the common end, so that the injection channel 31 and the common channel 33 are connected. The main control circuit controls the fourth drive circuit to make the second connecting valve 92 work in the cut-off state;

[0101] S3. The main control circuit controls the first drive circuit to start the first power pump 61. The main control circuit reads the value of the pressure sensor 63 and continuously adjusts the working state of the first power pump 61 so that the first power pump 61 operates within a preset pressure range.

[0102] S4: There is a pressure difference between the water sample and the liquid storage bottle 81. The water sample is sucked into the device, filtered by the coarse filter 21, and then enters the first connecting valve 30 and the filter chamber. The water sample passes through the fine filter membrane and flows into the liquid storage bottle 81. The fine filter membrane intercepts the target organisms in the water sample.

[0103] S5. The main control circuit controls the first drive circuit to keep the first power pump 61 working. The liquid level in the liquid storage bottle 81 continues to rise. The main control circuit reads the values ​​of the two liquid level sensors 82 until the two liquid level sensors 82 sense the liquid levels at the same time.

[0104] S6: The main control circuit controls the third drive circuit to switch the working state of the first connecting valve 30 to connect the air end 35 and the common end, so that the air inlet channel 32 and the common channel 33 are connected. At this time, the first power pump 61 is still working, and air enters the pipeline 101. The remaining water sample in the pipeline 101 gradually enters the liquid storage bottle 81;

[0105] S7, when all the water sample in the pipeline 101 enters the liquid storage bottle 81, air begins to enter the liquid storage bottle 81. When the air enters the liquid storage bottle 81, the main control circuit reads through the pressure sensor 63 that the pressure in the liquid storage bottle 81 gradually rises. When the pressure rises to the preset value, the first power pump 61 stops working;

[0106] S8. The main control circuit controls the data transmission circuit to send the filtration pressure and filtration time back to the shore server; so that the filtration pressure and filtration time can be selectively combined with the pre-established relationship between the filtration pressure, filtration time and the mass of the organisms on the fine filtration membrane, and the filtration pressure and filtration time can be used to assist in calculating the mass, thereby improving the monitoring accuracy.

[0107] At the same time, a data visualization platform (platform) can be built and configured based on the shore-based server to store the data transmitted by the device online and display it visually (for example, by plotting a visualization curve) to monitor whether the device is operating normally. Furthermore, a visualization curve with time as the horizontal axis and the quality of the collected organisms as the vertical axis can be drawn to monitor whether the sampling quality has a sudden increase or constant value in a short period of time, thereby determining whether there is an outbreak of organisms in a short period of time. The platform can then issue an early warning to guide on-site manual emergency monitoring and routine maintenance of on-site equipment.

[0108] After the automated collection is completed using the device, the controller can control the device to perform the dynamic weighing step, assuming that the device has performed at least one collection and the working state of the first connecting valve 30 is to connect the air end 35 and the common end. The specific steps include:

[0109] S1, the main control circuit controls the third drive circuit to switch the working state of the first connecting valve 30 to connect the air end 35 and the common end;

[0110] S2, the main control circuit reads the value of the pressure sensor 63 until the pressure in the liquid storage bottle 81 approaches one standard atmospheric pressure;

[0111] S3: The main control circuit controls the fifth drive circuit to operate the clamping assembly 50 in the loosened state. At this time, the first clamping member 52 and the second clamping member 53 release the fine filter membrane assembly 40, and the filter cavity is separated from the first connecting valve 30 and the liquid storage bottle 81, respectively. The fine filter membrane assembly 40 is connected to the weighing end 71 of the weighing assembly 70;

[0112] S4, the main control circuit reads the acceleration value a sensed by the acceleration sensor 73, and the main control circuit reads the pressure value F of the weighing sensor 72;

[0113] S5. Read once every 100 milliseconds. When the value of a is greater than or equal to 0.9g and less than or equal to 1.1g, mark the value an and Fn, where g is the acceleration due to gravity. It should be noted that the reading frequency needs to be adjusted according to factors such as the output frequency of the acceleration sensor 73 and the weighing sensor 72 in the specific embodiment, the water sample environment (such as the sea environment), etc. This embodiment does not specifically limit the number of readings and the time interval between two consecutive readings;

[0114] S6. When the number of a values ​​and F values ​​that meet the above conditions reaches 10, stop recording;

[0115] Among them, when the reading time exceeds 60 seconds but 10 values ​​that meet the above conditions are not obtained, the k values ​​closest to the above conditions are selected to make up the 10 values; it should be noted that the more samples there are, the closer the calculation result is to the actual mass of the target organism, but the longer it takes, and a timeout processing mechanism is also required to avoid unlimited reading. This embodiment only lists one example.

[0116] S7: The main control circuit controls the fifth drive circuit to operate the clamping assembly 50 in a clamped state. At this point, the first clamping member 52 and the second clamping member 53 clamp the fine filter membrane assembly 40, the first connecting valve 30, the filter chamber, and the liquid storage chamber are connected, and the fine filter membrane assembly 40 is connected to the weighing end 71 of the weighing assembly 70.

[0117] S8. Using the marked values ​​a1…a10 and f1…f10, calculate m1…m10 according to the formula m=F / a; then, calculate the average value of m1…m10 as the biomass collected this time;

[0118] S9, the main control circuit controls the data transmission circuit to send the collected biological data back to the onshore server;

[0119] After completing the automated collection and weighing, the water sample in the device can be emptied. The specific steps of emptying the liquid in the liquid storage bottle 81 include:

[0120] S1: The main control circuit controls the third drive circuit to switch the working state of the first connecting valve 30 to connect the air end 35 and the common end. The main control circuit controls the fourth drive circuit to make the second connecting valve 92 work in the conducting state.

[0121] S2, the main control circuit controls the fifth drive circuit to make the clamping claws of the clamping assembly 50 work in a relaxed state;

[0122] S3, the main control circuit controls the second drive circuit to make the second power pump 91 continue to work, and the liquid level in the liquid storage bottle 81 continues to drop. After a predetermined time, the second power pump 91 stops working;

[0123] S4. The main control circuit controls the fifth drive circuit to make the clamping claws of the clamping assembly 50 work in a clamping state, and the main control circuit controls the fourth drive circuit to make the second connecting valve 92 work in a cut-off state.

[0124] The above description is only a preferred embodiment of the present application and does not limit the patent scope of the present application. All equivalent structural transformations made based on the contents of the present application specification and drawings, or direct / indirect application in other related technical fields, are included in the patent protection scope of the present application.

Claims

1. A device for collecting and monitoring organisms in water samples, characterized in that: The device comprises a housing, a coarse filtration component, a first connecting valve, a fine filtration membrane component, a clamping component, a suction filtration component, a weighing component and a main control module. The interior of the housing forms a cavity, and the coarse filtration component is arranged outside the housing to perform coarse filtration on the water sample. The first connecting valve, the fine filtration membrane assembly, the clamping assembly, the suction filtration assembly, the weighing assembly, and the main control module are all disposed in the housing; the first connecting valve, the clamping assembly, the suction filtration assembly, and the weighing assembly are all electrically connected to the main control module, and the main control module is used to control the first connecting valve, the clamping assembly, the suction filtration assembly, and the weighing assembly; The clamping assembly includes a driving member, a first clamping member, and a second clamping member, wherein the driving member drives the first clamping member and the second clamping member to be connected to each other to clamp the fine filter membrane assembly or to be moved away from each other to release the fine filter membrane assembly; When the clamping assembly clamps the fine filter membrane assembly, the first clamping member, the second clamping member and the fine filter membrane assembly enclose a filter cavity, and the coarse filter assembly, the first connecting valve, the filter cavity and the suction filter assembly are sequentially connected through a pipeline to form a filtration collection system; When the clamping assembly releases the fine filter membrane assembly, the fine filter membrane assembly and the weighing assembly form a dynamic weighing system; Under the action of the suction filtration component, the water sample flows through the coarse filtration component, the first connecting valve and the filtration cavity in sequence, and the fine filtration membrane component filters the water sample in the filtration cavity and intercepts the target organisms in the water sample; The fine filter membrane assembly includes a filter membrane holder and a fine filter membrane, wherein the fine filter membrane is mounted on the filter membrane holder to intercept target organisms in the water sample; the first clamping member and the second clamping member clamp or release the filter membrane holder, and when the filter membrane holder is clamped, the first clamping member, the second clamping member and the filter membrane holder enclose the filter chamber to form the filter chamber; The weighing component includes a weighing sensor, a weighing end is provided on the weighing sensor, and the weighing end is connected to the filter membrane fixing frame. When the clamping component releases the fine filter membrane component, the weighing sensor is at least used to monitor the pressure value jointly applied to the weighing end by the fine filter membrane component and the target organism.

2. The device for collecting and monitoring organisms in water samples according to claim 1, characterized in that: The filter membrane fixing frame includes a pressing member and a supporting member. In the movement direction of the first clamping member, the fine filter membrane is arranged between the pressing member and the supporting member. The pressing member and the supporting member respectively press against two sides of the fine filter membrane to press and fix the fine filter membrane; the weighing end is connected to the supporting member.

3. The device for collecting and monitoring organisms in water samples according to claim 1, characterized in that: The weighing assembly includes an acceleration sensor and a connecting seat. The weighing sensor is installed in the shell through the connecting seat. The acceleration sensor is used to monitor the acceleration value in the same direction as the force direction of the weighing end.

4. The device for collecting and monitoring organisms in water samples according to any one of claims 1 to 3, characterized in that: The first connecting valve is a three-way valve, and is provided with an injection channel, an air intake channel, and a common channel. When one of the injection channel and the air intake channel is opened, the other is closed; The sample inlet channel is connected to the coarse filter assembly through a pipeline, the air inlet channel is connected to an air inlet pipe, one end of the air inlet pipe away from the air inlet channel extends out of the housing, and the common channel is connected to the filter cavity through a pipeline; When the sampling channel is opened, the suction filtration component allows the water sample to flow through the coarse filtration component and the first connecting valve in sequence and enter the filtration cavity; When the air inlet channel is opened, the suction filtration assembly allows air to enter the filter cavity from the air inlet pipe through the first connecting valve, so that the water sample passes through the fine filter membrane assembly.

5. The device for collecting and monitoring organisms in water samples according to claim 4, characterized in that: The filtration component includes a first power pump, an air extraction pipe and a pressure sensor. The water sample organism collection and monitoring device also includes a liquid storage component. The liquid storage component has a liquid storage cavity, which is connected to the filtration cavity through a pipeline. One end of the air extraction pipe is connected to the liquid storage cavity, and the other end of the air extraction pipe is connected to the first power pump. The pressure sensor is arranged on the air extraction pipe to monitor the pressure on the air extraction pipe.

6. The device for collecting and monitoring organisms in water samples according to claim 5, characterized in that: The first power pump has a first inlet and a first outlet, the housing is provided with an air outlet, an air outlet pipe is mounted on the air outlet, one end of the air outlet pipe is connected to the first outlet, and the other end of the air outlet pipe extends out of the housing; The liquid storage chamber is provided with an air extraction port, and one end of the air extraction pipe away from the first power pump is connected to the air extraction port; The filtration assembly further includes an air filter, which is disposed on the air intake pipe and communicates with the air intake passage through the air intake pipe; The housing is provided with an air inlet, and the air inlet pipe is installed at the air inlet; The shell is provided with a sampling port, and a sampling tube is installed on the sampling port. One end of the sampling tube is connected to the coarse filter component, and the other end of the sampling tube is communicated with the sampling channel.

7. The device for collecting and monitoring organisms in water samples according to claim 5, characterized in that: The shell is provided with a waste outlet, a waste outlet pipe is installed on the waste outlet, and the liquid storage cavity is provided with a sample outlet; The device for collecting and monitoring organisms in water samples also includes a sample discharge component, which includes a second power pump and a second connecting valve. One end of the second power pump is connected to the sample discharge port through the second connecting valve, and the other end of the second power pump is connected to one end of the waste pipe, and the other end of the waste pipe extends out of the shell.

8. The device for collecting and monitoring organisms in water samples according to claim 7, characterized in that: The liquid storage assembly further includes a liquid level sensor, which is arranged on the side wall of the liquid storage cavity and is used to monitor the amount of water sample in the liquid storage cavity; The liquid level sensor is also used to sense that when the liquid level of the water sample in the liquid storage chamber rises to a set height value, trigger the second power pump to start and the first power pump to stop.

9. A method for collecting and monitoring organisms in water samples, using the device for collecting and monitoring organisms in water samples according to any one of claims 1 to 8, characterized in that: include: The main control module controls the driving member to drive the first clamping member and the second clamping member to approach each other to clamp the fine filter membrane assembly, so that the first clamping member, the second clamping member and the fine filter membrane assembly are enclosed to form a filter cavity; The main control module controls the coarse filter component, the first connecting valve, the filter chamber and the suction filter component to be connected in sequence through pipelines; Under the action of the suction filtration component, the water sample flows through the coarse filtration component, the first connecting valve and the filtration cavity in sequence, and the fine filtration membrane component filters the water sample in the filtration cavity and intercepts the target organisms in the water sample; The main control module controls the driving member to drive the first clamping member and the second clamping member away from each other, so as to loosen the fine filter membrane assembly, so that the first clamping member and the second clamping member are separated from the fine filter membrane assembly; The main control module controls the weighing component to weigh the fine filtration membrane component and the target organism as a whole to obtain weighing data.

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