A Streptomyces rocheri strain and its application

Abstract

The present invention is applicable to the field of microbial technology, and provides a rochei Streptomyces strain and an application thereof. The rochei Streptomyces is named Streptomyces rochei ZN-L1, which was deposited in the China Center for Type Culture Collection on October 12, 2023, with a deposit number of CCTCC NO: M20231874. The present invention also provides a microbial agent, including the above-mentioned rochei Streptomyces strain. The present invention also provides an application of the above-mentioned lactobacillus or microbial agent in preventing and controlling crop diseases and an application of the above-mentioned lactobacillus or microbial agent in resisting continuous cropping. The rochei Streptomyces provided by the present invention is added to the soil, which will increase the beneficial bacteria in the soil and reduce the harmful bacteria, and can degrade phenolic acid self-toxic substances, and effectively prevent and control the continuous cropping obstacles in the soil.

Description

Technical Field

[0001] The invention belongs to the technical field of microorganisms, and in particular relates to a Streptomyces rocherii strain and application thereof. Background Art

[0002] Soil-borne crop diseases are a major obstacle to crop production. The application of chemical pesticides and other prevention and control measures generally has poor results, pollutes the environment, and induces plant pathogens to develop resistance. In addition, the resulting crop continuous cropping obstacles are becoming more and more serious, and have become a major problem restricting the improvement of agricultural quality and efficiency. Due to long-term continuous cropping and the use of pesticides, pathogens develop resistance and increased pathogenicity, causing the disease to spread and become rampant, thus causing serious economic losses to growers;

[0003] The present application provides a Streptomyces rocherii, which has the functions of preventing diseases and resisting repeated cropping. Summary of the invention

[0004] The present invention provides a Streptomyces rocherii, aiming to solve the above problems.

[0005] The present invention is achieved in that a Streptomyces rochei, named Streptomyces rochei ZN-L1, was deposited in the China Center for Type Culture Collection on October 12, 2023, with a deposit number of CCTCC NO: M 20231874.

[0006] The present invention also provides a microbial agent, comprising the above-mentioned Streptomyces rocherii strain.

[0007] Preferably, it also includes modified nano-colloidal magnesia and modified tourmaline.

[0008] Preferably, the mass ratio of the Streptomyces rocherii strain, the modified nano-colloidite and the modified tourmaline is 1:80-100:120-160.

[0009] Preferably, the preparation method of the modified nano-colloidal stone is as follows: the nano-colloidal stone is placed in a magnesium sulfate solution with a concentration of 30-40% and a weight of 5-8 times, and then soaked for 3-6 hours, taken out and filtered, washed with deionized water, dried and ground into nano powder, and then heated to 500-600°C, and steam is introduced to expand the pores, and after cooling, it is washed with water and dried to obtain the expanded pore nano-colloidal stone, the expanded pore nano-colloidal stone is placed in a culture vessel and injected with bacterial cellulose fermentation liquid, wrapped, and cultured at 25-30°C for 2-5 days, the culture product is rinsed with deionized water, the residual culture liquid is removed, and it is soaked in deionized water until it is colorless, and finally filtered and dried to obtain the modified nano-colloidal stone.

[0010] The surface of the nano-gelatite is modified by soaking in a magnesium sulfate solution, so that it is peeled and dispersed into finer single crystals, and then the pores are expanded by water vapor to make the nano-gelatite more dense and porous, with a larger specific surface area. During the fermentation process, bacterial cellulose grows into the nano-gelatite to form an interlaced interpenetrating network with a large storage space that can accommodate more microorganisms to grow and reproduce. It can adsorb and retain nutrients in the soil to prevent them from being lost, so that the soil can better provide nutrients to plants and promote plant growth and development. It can also adsorb and fix harmful substances to avoid their harm to plants, which helps maintain the health of the soil ecological environment.

[0011] Preferably, the preparation method of the bacterial cellulose fermentation liquid is as follows: inoculating Gluconacetobacter species into the culture liquid, the temperature is 24-28°C, the stirring speed is 200-260r / min, and the culture time is 24-36 hours to obtain seed liquid; the seed liquid with a volume of 10-15% of the culture liquid is introduced into the culture liquid, and mixed to obtain the bacterial cellulose fermentation liquid.

[0012] Preferably, the preparation method of the modified tourmaline is as follows: take tourmaline, clean the surface, dry it and then perform preliminary crushing, place it in a muffle furnace and heat it to 600-800°C, constant temperature roasting for 1-2h, then put it into -40~-50°C for freezing for 1-2h, place it in a muffle furnace again and heat it to 1000-1200°C, constant temperature roasting for 1-2h to obtain powder, then add hypochlorous acid with a mass fraction of 8-12% and 15-25 times the mass of the powder, heat it in a water bath at 30-40°C and stir it thoroughly for 40-60min, adjust the pH to 7.0, add octadecyltrimethylammonium chloride solution with a mass of 10-15 times the mass of the powder and a concentration of 0.2-0.4mmol / L, heat it to 70-90°C and react for 30-50min, filter it, wash it with distilled water for 3 times, dry it at 100°C, and grind it to pass through a 200-mesh sieve to obtain modified tourmaline.

[0013] Tourmaline's spontaneous polarization, continuous generation of DC static electricity, far-infrared radiation, release of negative ions, release of minerals and trace elements and other properties are utilized to assist microorganisms in improving soil control effects. Tourmaline is broken into a finer structure with more pores through roasting, freezing and re-roasting. Hypochlorous acid is added, heated and stirred, and the hypochlorous acid decomposes to produce oxygen and hydrochloric acid. The hydrochloric acid immersion causes the tourmaline crystals to break, presenting porous characteristics, and impurities in the particle channels are dissolved, the adsorption capacity is improved, and the generated oxygen forms small bubbles that enter the interlayer of tourmaline, generating pressure, so that the wall layer of tourmaline has a larger space, which can adsorb more harmful substances. The tourmaline is surface modified by octadecyltrimethylammonium chloride to further increase its surface energy.

[0014] The present invention also provides a method for preparing the above-mentioned microbial agent, comprising the following steps:

[0015] Prepare the raw materials according to the ratio;

[0016] Streptomyces rocherii activated;

[0017] Streptomyces rocherii enrichment;

[0018] Streptomyces rocherii culture expansion

[0019] The enlarged seed liquid is mixed with modified nano-colloidal ling stone and modified tourmaline to obtain a microbial agent.

[0020] The present invention also provides application of the lactobacillus or microbial agent in preventing and controlling crop diseases.

[0021] The present invention also provides application of the lactobacillus or microbial agent in resisting continuous cropping.

[0022] Compared with the prior art, the embodiments of the present application have the following beneficial effects:

[0023] When the Streptomyces rocherii provided by the present invention is added to the soil, the beneficial bacteria in the soil will increase and the harmful bacteria will decrease, and the phenolic acid self-toxic substances can be degraded, so as to effectively prevent and control the continuous cropping obstacle in the soil.

[0024] The microbial agent provided by the present invention comprises Streptomyces rocherii, modified nano-colloidal stone and modified tourmaline. By adding the modified nano-colloidal stone, the agent has a large storage space, can accommodate more microorganisms to grow and reproduce, can adsorb and retain nutrients in the soil, and make it difficult for the nutrients to be lost, so that the soil can better provide nutrients for plants and promote the growth and development of plants. It can also adsorb and fix harmful substances to avoid their harm to plants, which is helpful to maintain the health of the soil ecological environment. By adding the modified tourmaline, the agent has a large surface energy and excellent adsorption performance, can adsorb more harmful substances and kill harmful microorganisms, has the properties of spontaneous polarization, continuous generation of direct current static electricity, radiation of far infrared rays, release of negative ions, release of minerals and trace elements, etc., assists microorganisms to improve the soil prevention and control effect, and the modified nano-colloidal stone and modified tourmaline have a synergistic effect, which can further improve the disease prevention and anti-replanting effects of the microbial agent. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 The present invention provides a preparation flow chart of a microbial agent. DETAILED DESCRIPTION

[0026] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by technicians in the technical field of this application; the terms used in the specification of the application herein are only for the purpose of describing specific embodiments and are not intended to limit this application; the terms "including" and "having" and any variations thereof in the specification and claims of this application and the above-mentioned drawings are intended to cover non-exclusive inclusions. The terms "first", "second", etc. in the specification and claims of this application or the above-mentioned drawings are used to distinguish different objects, not to describe a specific order.

[0027] Reference to "embodiments" herein means that a particular feature, structure, or characteristic described in conjunction with the embodiments may be included in at least one embodiment of the present application. The appearance of the phrase in various locations in the specification does not necessarily refer to the same embodiment, nor is it an independent or alternative embodiment that is mutually exclusive with other embodiments. It is explicitly and implicitly understood by those skilled in the art that the embodiments described herein may be combined with other embodiments.

[0028] Example 1

[0029] An embodiment of the present invention provides a Streptomyces rochei, named Streptomyces rochei ZN-L1, which was deposited in the China Center for Type Culture Collection on October 12, 2023, with a deposit number of CCTCC NO: M 20231874.

[0030] Example 2

[0031] The present invention provides a microbial agent, comprising the above-mentioned Streptomyces rocherii strain, and also comprising modified nano-colloidite and modified tourmaline, wherein the mass ratio of the Streptomyces rocherii strain, the modified nano-colloidite and the modified tourmaline is 1:80:120. Figure 1 As shown, the preparation method of the microbial agent comprises the following steps:

[0032] Prepare the raw materials according to the ratio;

[0033] Streptomyces rocherii activated;

[0034] Streptomyces rocherii enrichment;

[0035] Streptomyces rocherii culture expansion

[0036] The enlarged seed liquid is mixed with modified nano-colloidal ling stone and modified tourmaline to obtain a microbial agent.

[0037] The preparation method of the modified nano-gelatite is as follows: the nano-gelatite is placed in a magnesium sulfate solution with a concentration of 30% and a weight of 5 times the weight of the magnesium sulfate solution for 3 hours, then taken out and filtered, washed with deionized water, dried and ground into nano powder, then heated to 500°C, and steam is introduced to expand the pores, cooled and washed with water and dried to obtain the expanded nano-gelatite, the expanded nano-gelatite is placed in a culture vessel and injected with bacterial cellulose fermentation liquid, wrapped, and cultured at 25°C for 2 days, the culture product is rinsed with deionized water, the residual culture liquid is removed, and it is soaked in deionized water until it is colorless, and finally filtered and dried to obtain the modified nano-gelatite.

[0038] In this embodiment, the preparation method of the bacterial cellulose fermentation liquid is as follows: inoculating Gluconacetobacter species into the culture liquid, the temperature is 24°C, the stirring speed is 200r / min, and the culture time is 24 hours to obtain a seed liquid; the seed liquid with a volume of 10% of the culture liquid is added to the culture liquid, and mixed to obtain a bacterial cellulose fermentation liquid.

[0039] In a specific implementation, the preparation method of the modified tourmaline is as follows: take tourmaline, clean the surface, dry it and then perform preliminary crushing, place it in a muffle furnace and heat it to 600°C, constant temperature roasting for 1h, then put it into -40°C for freezing for 1h, put it into the muffle furnace again and heat it to 1000°C, constant temperature roasting for 1h to obtain powder, then add hypochlorous acid with a mass fraction of 8% and 15 times the mass of the powder, heat it in a water bath at 30°C and stir it thoroughly for 40min, adjust the pH to 7.0, add octadecyltrimethylammonium chloride solution with a concentration of 0.2.4mmol / L and 10 times the mass of the powder, heat it to 70°C and react for 30min, filter it, wash it with distilled water for 3 times, dry it at 100°C, and grind it to pass through a 200-mesh sieve to obtain modified tourmaline.

[0040] Example 3

[0041] The present invention provides a microbial agent, comprising the above-mentioned Streptomyces rocherii strain, and also comprising modified nano-colloidite and modified tourmaline, wherein the mass ratio of the Streptomyces rocherii strain, the modified nano-colloidite and the modified tourmaline is 1:85:130, and the preparation method of the microbial agent comprises the following steps:

[0042] Prepare the raw materials according to the ratio;

[0043] Streptomyces rocherii activated;

[0044] Streptomyces rocherii enrichment;

[0045] Streptomyces rocherii culture expansion

[0046] The enlarged seed liquid is mixed with modified nano-colloidal ling stone and modified tourmaline to obtain a microbial agent.

[0047] The preparation method of the modified nano-gelatite is as follows: the nano-gelatite is placed in a magnesium sulfate solution with a concentration of 30% and a weight of 5 times the weight of the magnesium sulfate solution for 3 hours, then taken out and filtered, washed with deionized water, dried and ground into nano powder, then heated to 500°C, and steam is introduced to expand the pores, cooled and washed with water and dried to obtain the expanded nano-gelatite, the expanded nano-gelatite is placed in a culture vessel and injected with bacterial cellulose fermentation liquid, wrapped, and cultured at 25°C for 2 days, the culture product is rinsed with deionized water, the residual culture liquid is removed, and it is soaked in deionized water until it is colorless, and finally filtered and dried to obtain the modified nano-gelatite.

[0048] In this embodiment, the preparation method of the bacterial cellulose fermentation liquid is as follows: inoculating Gluconacetobacter species into the culture liquid, the temperature is 24°C, the stirring speed is 200r / min, and the culture time is 24 hours to obtain a seed liquid; the seed liquid with a volume of 10% of the culture liquid is added to the culture liquid, and mixed to obtain a bacterial cellulose fermentation liquid.

[0049] In a specific implementation, the preparation method of the modified tourmaline is as follows: take tourmaline, clean the surface, dry it and then perform preliminary crushing, place it in a muffle furnace and heat it to 600°C, constant temperature roasting for 1h, then put it into -40°C for freezing for 1h, put it into the muffle furnace again and heat it to 1000°C, constant temperature roasting for 1h to obtain powder, then add hypochlorous acid with a mass fraction of 8% and 15 times the mass of the powder, heat it in a water bath at 30°C and stir it thoroughly for 40min, adjust the pH to 7.0, add octadecyltrimethylammonium chloride solution with a concentration of 0.2.4mmol / L and 10 times the mass of the powder, heat it to 70°C and react for 30min, filter it, wash it with distilled water for 3 times, dry it at 100°C, and grind it to pass through a 200-mesh sieve to obtain modified tourmaline.

[0050] Example 4

[0051] The present invention provides a microbial agent, comprising the above-mentioned Streptomyces rocherii strain, and also comprising modified nano-colloidite and modified tourmaline, wherein the mass ratio of the Streptomyces rocherii strain, the modified nano-colloidite and the modified tourmaline is 1:90:140, and the preparation method of the microbial agent comprises the following steps:

[0052] Prepare the raw materials according to the ratio;

[0053] Streptomyces rocherii activated;

[0054] Streptomyces rocherii enrichment;

[0055] Streptomyces rocherii culture expansion

[0056] The enlarged seed liquid is mixed with modified nano-colloidal ling stone and modified tourmaline to obtain a microbial agent.

[0057] The preparation method of the modified nano-colloidal silicate is as follows: the nano-colloidal silicate is placed in a magnesium sulfate solution with a concentration of 35% and a weight of 5-8 times and soaked for 4.5 hours, taken out and filtered, washed with deionized water, dried and ground into nano-powder, and then heated to 550°C, and steam is introduced to expand the pores. After cooling, it is washed with water and dried to obtain the expanded pore nano-colloidal silicate. The expanded pore nano-colloidal silicate is placed in a culture vessel and injected with bacterial cellulose fermentation liquid, wrapped, and cultured at 28°C for 3.5 days. The culture product is rinsed with deionized water, the residual culture liquid is removed, and it is soaked with deionized water until it is colorless. Finally, it is filtered and dried to obtain the modified nano-colloidal silicate.

[0058] In this embodiment, the preparation method of the bacterial cellulose fermentation liquid is as follows: inoculating Gluconacetobacter species into the culture liquid, the temperature is 26°C, the stirring speed is 230r / min, and the culture time is 30 hours to obtain a seed liquid; the seed liquid with a volume of 12% of the culture liquid is added to the culture liquid, and mixed to obtain a bacterial cellulose fermentation liquid.

[0059] In a specific implementation, the preparation method of the modified tourmaline is as follows: take tourmaline, clean the surface, dry it and then perform preliminary crushing, place it in a muffle furnace and heat it to 700°C, constant temperature roasting for 1.5 hours, then put it into -45°C for freezing for 1.5 hours, put it into the muffle furnace again and heat it to 1000-1200°C, constant temperature roasting for 1.5 hours to obtain powder, then add hypochlorous acid with a mass fraction of 10% and 20 times the mass of the powder, heat it in a water bath at 35°C and stir it thoroughly for 50 minutes, adjust the pH to 7.0, add octadecyltrimethylammonium chloride solution with a concentration of 0.3mmol / L and 13 times the mass of the powder, heat it to 80°C and react for 40 minutes, filter it, wash it with distilled water for 3 times, dry it at 100°C, and grind it to pass through a 200-mesh sieve to obtain modified tourmaline.

[0060] Example 5

[0061] The present invention provides a microbial agent, comprising the above-mentioned Streptomyces rocherii strain, and also comprising modified nano-colloidite and modified tourmaline, wherein the mass ratio of the Streptomyces rocherii strain, the modified nano-colloidite and the modified tourmaline is 1:95:150, and the preparation method of the microbial agent comprises the following steps:

[0062] Prepare the raw materials according to the ratio;

[0063] Streptomyces rocherii activated;

[0064] Streptomyces rocherii enrichment;

[0065] Streptomyces rocherii culture expansion

[0066] The enlarged seed liquid is mixed with modified nano-colloidal ling stone and modified tourmaline to obtain a microbial agent.

[0067] The preparation method of the modified nano-colloidal silicate is as follows: the nano-colloidal silicate is placed in a magnesium sulfate solution with a concentration of 40% and 8 times the weight of the solution and soaked for 6 hours, then taken out and filtered, washed with deionized water, dried and ground into nano-powder, and then heated to 600°C, and steam is introduced to expand the pores, and then cooled and washed and dried to obtain the expanded nano-colloidal silicate, the expanded nano-colloidal silicate is placed in a culture vessel and injected with bacterial cellulose fermentation liquid, wrapped, and cultured at 30°C for 5 days, the culture product is rinsed with deionized water, the residual culture liquid is removed, and it is soaked in deionized water until it is colorless, and finally filtered and dried to obtain the modified nano-colloidal silicate.

[0068] In this embodiment, the preparation method of the bacterial cellulose fermentation liquid is as follows: inoculating Gluconacetobacter species into the culture liquid, the temperature is 28°C, the stirring speed is 260r / min, and the culture time is 36 hours to obtain a seed liquid; the seed liquid with a volume of 15% of the culture liquid is added to the culture liquid, and mixed to obtain a bacterial cellulose fermentation liquid.

[0069] In a specific implementation, the preparation method of the modified tourmaline is as follows: take tourmaline, clean the surface, dry it and then perform preliminary crushing, place it in a muffle furnace and heat it to 800°C, constant temperature roast it for 2h, then put it into -50°C for freezing for 2h, put it into the muffle furnace again and heat it to 1200°C, constant temperature roast it for 2h to obtain powder, then add hypochlorous acid with a mass fraction of 12% and 25 times the mass of the powder, heat it in a water bath at 40°C and stir it thoroughly for 60min, adjust the pH to 7.0, add octadecyltrimethylammonium chloride solution with a concentration of 0.0.4mmol / L and 15 times the mass of the powder, heat it to 90°C and react for 50min, filter it, wash it with distilled water for 3 times, dry it at 100°C, and grind it to pass through a 200-mesh sieve to obtain modified tourmaline.

[0070] Example 6

[0071] The present invention provides a microbial agent, comprising the above-mentioned Streptomyces rocherii strain, and also comprising modified nano-colloidite and modified tourmaline, wherein the mass ratio of the Streptomyces rocherii strain, the modified nano-colloidite and the modified tourmaline is 1:100:160, and the preparation method of the microbial agent comprises the following steps:

[0072] Prepare the raw materials according to the ratio;

[0073] Streptomyces rocherii activated;

[0074] Streptomyces rocherii enrichment;

[0075] Streptomyces rocherii culture expansion

[0076] The enlarged seed liquid is mixed with modified nano-colloidal ling stone and modified tourmaline to obtain a microbial agent.

[0077] The preparation method of the modified nano-colloidal silicate is as follows: the nano-colloidal silicate is placed in a magnesium sulfate solution with a concentration of 40% and 8 times the weight of the solution and soaked for 6 hours, then taken out and filtered, washed with deionized water, dried and ground into nano-powder, and then heated to 600°C, and steam is introduced to expand the pores, and then cooled and washed and dried to obtain the expanded nano-colloidal silicate, the expanded nano-colloidal silicate is placed in a culture vessel and injected with bacterial cellulose fermentation liquid, wrapped, and cultured at 30°C for 5 days, the culture product is rinsed with deionized water, the residual culture liquid is removed, and it is soaked in deionized water until it is colorless, and finally filtered and dried to obtain the modified nano-colloidal silicate.

[0078] In this embodiment, the preparation method of the bacterial cellulose fermentation liquid is as follows: inoculating Gluconacetobacter species into the culture liquid, the temperature is 28°C, the stirring speed is 260r / min, and the culture time is 36 hours to obtain a seed liquid; the seed liquid with a volume of 15% of the culture liquid is added to the culture liquid, and mixed to obtain a bacterial cellulose fermentation liquid.

[0079] In a specific implementation, the preparation method of the modified tourmaline is as follows: take tourmaline, clean the surface, dry it and then perform preliminary crushing, place it in a muffle furnace and heat it to 800°C, constant temperature roast it for 2h, then put it into -50°C for freezing for 2h, put it into the muffle furnace again and heat it to 1200°C, constant temperature roast it for 2h to obtain powder, then add hypochlorous acid with a mass fraction of 12% and 25 times the mass of the powder, heat it in a water bath at 40°C and stir it thoroughly for 60min, adjust the pH to 7.0, add octadecyltrimethylammonium chloride solution with a concentration of 0.0.4mmol / L and 15 times the mass of the powder, heat it to 90°C and react for 50min, filter it, wash it with distilled water for 3 times, dry it at 100°C, and grind it to pass through a 200-mesh sieve to obtain modified tourmaline.

[0080] Comparative Example 1: The difference from Example 4 is that the modified tourmaline is replaced by ordinary tourmaline.

[0081] Comparative Example 2: The difference from Example 4 is that the modified nano-colloidal ore is replaced by ordinary nano-colloidal ore.

[0082] Comparative Example 3: The difference from Example 4 is that the modified tourmaline is replaced by ordinary tourmaline and the modified nano-colloidal ore is replaced by ordinary nano-colloidal ore.

[0083] Comparative Example 4: A commercially available disease prevention and anti-replanting fungicide.

[0084] Experiment 1

[0085] The control effects of the bacterial agents of Examples 2-6 and Comparative Examples 1-4 on potato wilt and tomato bacterial wilt were determined through pot plant experiments.

[0086] Each group of microbial agents was applied in greenhouses where potatoes and tomatoes were planted. 100g was applied per square meter, evenly spread or buried 15cm below the soil, away from the roots. Through the recording and statistics of the disease conditions of each crop, the experimental results are shown in Table 1:

[0087] Group Potato wilt control effect (%) Tomato bacterial wilt control effect (%) Example 2 81.3 80.1 Example 3 81.7 80.3 Example 4 82.8 80.5 Example 5 82.5 80.4 Example 6 81.9 80.2 Comparative Example 1 71.2 66.4 Comparative Example 2 72.6 68.5 Comparative Example 3 65.5 60.7 Comparative Example 4 62.1 57.5

[0088] From the above results, it can be seen that the microbial agent prepared by the present invention has good preventive effect on potato wilt and tomato bacterial wilt, especially by adding modified nano-colloidite and modified tourmaline, the two have a synergistic effect and can assist microorganisms to further improve the disease prevention effect.

[0089] Experiment 2

[0090] The experiment was conducted at a tomato planting base, where tomatoes are planted all year round, and the phenomenon of repeated cropping is quite typical. The soil in the middle of each greenhouse was selected for the test, and the test variety was "Colorful". A treatment group and a control group were set up, each with an area of ​​50m 2 , the most typical double-bed high ridge cultivation mode was adopted. The microbial agents of Examples 2-6 and Comparative Examples 1-4 were used as the treatment group, and the soil without any microbial agent was used as the control group. The microbial agent was applied to the treatment group at an application dose of 80 kg / mu, and then the soil was turned over by a rotary tiller. The cultivation and management of tomatoes were carried out in a normal manner. The experiment lasted for 6 months. The weight of the harvested tomatoes was recorded after each picking until the end of the experiment. The greenhouse yield data was accumulated. The weighing tool was an ordinary electronic scale. The experimental results are shown in Table 2 below:

[0091] Group Output kg Control group 880.6 Example 2 1108.9 Example 3 1110.5 Example 4 1115.7 Example 5 1112.4 Example 6 1109.6 Comparative Example 1 1015.6 Comparative Example 2 1023.8 Comparative Example 3 962.5 Comparative Example 4 955.8

[0092] It can be seen from the above results that the bacterial agent prepared by the present invention can promote the growth of crops, increase crop yields, and can significantly eliminate the harm of repeated cropping in the soil. By adding modified nano-colloidal lingaite and modified tourmaline, the two have a synergistic effect and can assist microorganisms to further improve the anti-repeated cropping effect.

[0093] It should be noted that, for the above embodiments, for the sake of simplicity, they are all described as a series of action combinations, but those skilled in the art should know that the present invention is not limited by the described order of actions, because according to the present invention, some steps may be performed in other orders or simultaneously. Secondly, those skilled in the art should also know that the embodiments described in the specification are all preferred embodiments, and the actions and modules involved are not necessarily required by the present invention.

[0094] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the scope of protection of the invention. Obviously, the described embodiments are only some embodiments of the present invention, rather than all embodiments. Based on these embodiments, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention. Although the present invention has been described in detail with reference to the above embodiments, ordinary technicians in this field can still combine, add, delete or make other adjustments to the features in the various embodiments of the present invention according to the circumstances without conflict, without making creative work, so as to obtain different other technical solutions that do not deviate from the concept of the present invention in essence, and these technical solutions also belong to the scope of protection of the present invention.

Claims

1. A microbial agent, characterized in that: The invention relates to a Streptomyces loucherii strain, named Streptomyces loucherii ZN-L1, which was deposited in the China Center for Type Culture Collection on October 12, 2023, with a deposit number of CCTCC NO: M20231874, and also comprises modified nano-colloidal lingyaite and modified tourmaline, wherein the mass ratio of the Streptomyces loucherii strain, the modified nano-colloidal lingyaite and the modified tourmaline is 1:80-100:120-160, and the preparation method of the modified nano-colloidal lingyaite is as follows: the nano-colloidal lingyaite is placed in a magnesium sulfate solution with a concentration of 30-40% and a weight of 5-8 times, and then taken out and filtered, washed with deionized water, and ground into nano-powder after drying, and then heated to 500-600°C, and water vapor is introduced for pore expansion, and after cooling, it is washed with water and dried to obtain the pore-expanded nano-colloidal lingyaite, and the pore-expanded nano-colloidal lingyaite is obtained. The colloid stone is placed in a culture vessel and injected with bacterial cellulose fermentation liquid, which is then wrapped and cultured at 25-30° C. for 2-5 days. The culture product is rinsed with deionized water, the residual culture liquid is removed, and the product is soaked with deionized water until it is colorless. Finally, the modified nano-colloid stone is filtered and dried to obtain the modified nano-colloid stone. The preparation method of the bacterial cellulose fermentation liquid is as follows: inoculating Gluconacetobacter species into the culture liquid at a temperature of 24-28° C., a stirring speed of 200-260 r / min, and a culture time of 24-36 hours to obtain a seed liquid; the seed liquid having a volume of 10-15% of the culture liquid is introduced into the culture liquid, and the mixture is mixed to obtain a bacterial cellulose fermentation liquid; The preparation method of the modified tourmaline is as follows: taking tourmaline, cleaning the surface, drying and then performing preliminary crushing, placing in a muffle furnace and heating to 600-800° C., constant temperature roasting for 1-2 hours, then placing in a -40--50° C. and freezing for 1-2 hours, placing in a muffle furnace again and heating to 1000-1200° C., constant temperature roasting for 1-2 hours to obtain powder, then adding hypochlorous acid with a mass fraction of 8-12% and 15-25 times the mass of the powder, heating in a water bath at 30-40° C. and fully stirring for 40-60 minutes, adjusting the pH to 7.0, adding octadecyltrimethylammonium chloride solution with a mass of 0.2-0.4 mmol / L and 10-15 times the mass of the powder, heating to 70-90° C. and reacting for 30-50 minutes, filtering, washing with distilled water for 3 times, drying at 100° C., and grinding until passing through a 200-mesh sieve to obtain the modified tourmaline.

2. The method for preparing the microbial agent according to claim 1, characterized in that: The steps include: Prepare the raw materials according to the ratio; Streptomyces rocherii activated; Streptomyces rocherii enrichment; Streptomyces rocherii culture expansion The enlarged seed liquid is mixed with modified nano-colloidal ling stone and modified tourmaline to obtain a microbial agent.

3. Use of the microbial agent as claimed in claim 1 or 2 in the prevention and control of potato wilt and tomato bacterial wilt.

4. Use of the microbial agent as claimed in claim 1 or 2 in increasing tomato yield.

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