Vaginal antibacterial liquid and preparation method thereof

Abstract

The present invention relates to the technical field of gynecological recuperation, and specifically provides a vaginal antibacterial liquid and a preparation method thereof, wherein the vaginal antibacterial liquid comprises: a nourishing extract with a mass fraction of 40-53%, an antibacterial extract with a mass fraction of 27-38%, nisin with a mass fraction of 2-4%, and pure water to make up the remaining mass fraction. The present invention combines traditional Chinese medicine that directly inhibits and kills bacteria with nisin, and with traditional Chinese medicine that improves vaginal blood circulation and glandular secretion, to treat both the symptoms and the root causes, so that foreign natural medicinal materials and self-immunity jointly inhibit and kill harmful bacteria.

Description

Technical Field

[0001] The invention relates to the technical field of gynecological recuperation, and in particular to a vaginal antibacterial liquid and a preparation method thereof. Background Art

[0002] Vaginal diseases, especially bacterial vaginosis, are important targets of gynecological treatment; the temperature and humidity environment of the vagina easily cause the growth and reproduction of a variety of pathogens, and some protein-rich vaginal secretions also accelerate the reproduction of pathogens; common pathogens of bacterial vaginitis include Gram-negative bacteria represented by Escherichia coli and Gram-positive bacteria represented by Staphylococcus aureus.

[0003] Currently, the mainstream lotion uses metronidazole and chlorhexidine, but on the one hand, some people may have an allergic reaction to the metronidazole and chlorhexidine lotion, and the vagina may experience burning sensation or redness and swelling. On the other hand, in addition to causing gastrointestinal irritation such as nausea and vomiting, metronidazole can also have a certain damaging effect on the heart, liver, kidneys and blood system. If used for a long time and the drug accumulates in the body, it cannot be ruled out that these adverse reactions may occur.

[0004] Therefore, there is a need for a vaginal antibacterial liquid with natural ingredients that can inhibit and kill bacteria and a preparation method thereof. Summary of the invention

[0005] In order to solve the above problems, the present invention aims to provide a vaginal antibacterial liquid and a preparation method thereof.

[0006] In one aspect, the present invention provides a method for preparing a vaginal antibacterial liquid, the method for preparing the vaginal antibacterial liquid comprising the following steps:

[0007] Step 1: Take 42-58% of Rehmannia glutinosa, 21-30% of Salvia miltiorrhiza and pure water to make up the rest, cut them into pieces and pass through a 50-mesh sieve, add 80% ethanol with a mass of 10 times the mass of the Rehmannia glutinosa and Salvia miltiorrhiza, immerse them at 78°C for 24 hours, add them into a flash extractor with a speed of 8000 r / min, extract for 30 seconds and filter to obtain a coarse filtrate extract; add 0.2 times the mass of pure water to the coarse filtrate extract and distill it under reduced pressure at 69°C and 800 mbar for 20 minutes, collect the remaining liquid, add 88% lactic acid to adjust the pH to 3.5 to obtain a nourishing extract;

[0008] Step 2: Take 30-44% of Sophora flavescens by mass, 28-40% of Coptis chinensis by mass and make up the rest with pure water, cut them into pieces and pass through a 50-mesh sieve, add 80% ethanol with a mass of 10 times the mass of the Sophora flavescens and the Coptis chinensis, immerse them at 56°C for 24 hours, add them into a flash extractor with a speed of 5000r / min, extract for 30s and filter to obtain a coarse filtrate extract; add 0.2 times the mass of pure water to the coarse filtrate extract and distill it under reduced pressure at 60°C and 800mbar for 20min, collect the remaining liquid, add 88% lactic acid to adjust the pH to 3.5 to obtain an antibacterial extract;

[0009] Step 3: Mix 40-53% of the nourishing extract, 27-38% of the antibacterial extract, 2-4% of the nisin and pure water to make up the rest to obtain a vaginal antibacterial solution.

[0010] Furthermore, the mass fraction of the Rehmannia root prepared is 51%.

[0011] Furthermore, the mass fraction of the Salvia miltiorrhiza is 26%.

[0012] Furthermore, the mass fraction of the Sophora flavescens is 38%.

[0013] Furthermore, the mass fraction of the Coptis chinensis is 35%.

[0014] Furthermore, the mass fraction of the nourishing extract is 47%.

[0015] Furthermore, the mass fraction of the antibacterial extract is 32%.

[0016] Furthermore, the mass fraction of nisin is 3%.

[0017] On the other hand, the present invention provides a vaginal antibacterial liquid prepared by the preparation method of the vaginal antibacterial liquid as described above, wherein the vaginal antibacterial liquid comprises: 40-53% by mass of the nourishing extract, 27-38% by mass of the antibacterial extract, 2-4% by mass of the nisin, and pure water to make up the remaining mass fraction.

[0018] The present invention can bring the following beneficial effects:

[0019] The vaginal antibacterial liquid of the invention forms a unique nourishing-antibacterial system. The nourishing extract of the invention is extracted by a specific process with a specific proportion of Radix Rehmanniae and Radix Salviae Miltiorrhizae to work synergistically, nourishes kidney yin and improves local blood circulation to promote the secretion of the vaginal glands such as the Bartholin's glands containing lactobacilli, lysozyme and antibodies to inhibit and kill bacteria; the antibacterial extract of the invention is extracted by a specific process with a specific proportion of Radix Sophorae Flavescentis and the Rhizoma Coptidis to work synergistically to produce a relatively broad-spectrum antibacterial effect; the nisin of the invention is a natural bacteriocin from lactic acid bacteria, which can inhibit and kill bacteria such as Staphylococcus aureus; the three cooperate with each other to inhibit and kill harmful vaginal bacteria under the process of the invention.

[0020] The invention combines nisin with Chinese medicine that can directly inhibit and kill bacteria, and Chinese medicine that can improve vaginal blood circulation and glandular secretion, so as to treat both the symptoms and the root causes, and make the foreign natural medicine and the body's own immunity inhibit and kill harmful bacteria together. DETAILED DESCRIPTION

[0021] In order to more clearly explain the overall concept of the present invention, the overall scheme of the present invention is described in detail in the form of embodiments below; in the following description, a large number of specific details are given to provide a more thorough understanding of the present invention; however, it is obvious to those skilled in the art that the present invention can be implemented without one or more of these details; in other examples, in order to avoid confusion with the present invention, some technical features known in the art are not described.

[0022] In the present invention, the flash extractor was purchased from Beijing Haifuda Technology Co., Ltd., with a power of 1800W; the vacuum distiller was purchased from Zhengzhou Huachen Instrument Co., Ltd., model EXRE-1005. The water content of Rehmannia glutinosa, Salvia miltiorrhiza, Sophora flavescens and Coptis chinensis was 10%, all purchased from Bozhou Hancaoyuan Pharmaceutical Co., Ltd. Lactic acid with a content of 88% was purchased from Guangzhou Suixin Chemical Co., Ltd.; Nisin was purchased from Wuhan Lanabai Pharmaceutical Chemical Co., Ltd., item number LW341, CAS number: 1414-45-5.

[0023] Unless otherwise specified, all raw material components in the following examples can be purchased from commercial sources, the experimental instruments used are conventional laboratory experimental instruments, and the performance test methods are known in the art. The overall space environment is 25°C and the air humidity is 30%.

[0024] The preferred implementation is as follows:

[0025] Embodiment 1:

[0026] The vaginal antibacterial liquid is prepared by using raw materials including the following proportions:

[0027] 47% by mass of the nourishing extract, 32% by mass of the antibacterial extract, 3% by mass of the nisin, and the remaining amount of pure water;

[0028] The preparation method of the vaginal antibacterial liquid is as follows:

[0029] Step 1: Take 51% of Rehmannia glutinosa, 26% of Salvia miltiorrhiza and pure water to make up the rest, cut them into pieces and pass through a 50-mesh sieve, add 80% ethanol with a mass of 10 times the mass of the Rehmannia glutinosa and Salvia miltiorrhiza, immerse them at 78°C for 24 hours, add them into a flash extractor with a speed of 8000r / min, extract for 30 seconds and filter to obtain a coarse filtrate extract; add 0.2 times the mass of pure water to the coarse filtrate extract and distill it under reduced pressure at 69°C and 800mbar for 20 minutes, collect the remaining liquid, add 88% lactic acid to adjust the pH to 3.5 to obtain a nourishing extract;

[0030] Step 2: Take 38% of Sophora flavescens by mass, 35% of Coptis chinensis by mass and pure water to make up the rest, cut them into pieces and pass through a 50-mesh sieve, add 80% ethanol with a mass of 10 times the mass of the Sophora flavescens and the Coptis chinensis, immerse them at 56°C for 24 hours, add them into a flash extractor with a speed of 5000r / min, extract for 30 seconds and then filter to obtain a coarse filtrate extract; add 0.2 times the mass of pure water to the coarse filtrate extract and distill it under reduced pressure at 60°C and 800mbar for 20 minutes, collect the remaining liquid, add 88% lactic acid to adjust the pH to 3.5 to obtain an antibacterial extract;

[0031] Step 3: Mix 47% of the nourishing extract, 32% of the antibacterial extract, 3% of the nisin and the remaining amount of pure water to obtain a vaginal antibacterial solution.

[0032] Embodiment 2-15:

[0033] The only difference between Example 2 and Example 1 is that the mass fraction of Rehmannia glutinosa is 42%;

[0034] The only difference between Example 3 and Example 1 is that the mass fraction of Rehmannia glutinosa is 58%;

[0035] The only difference between Example 4 and Example 1 is that the mass fraction of Salvia miltiorrhiza is 21%;

[0036] The only difference between Example 5 and Example 1 is that the mass fraction of Salvia miltiorrhiza is 30%;

[0037] The only difference between Example 6 and Example 1 is that the mass fraction of Sophora flavescens is 30%;

[0038] The only difference between Example 7 and Example 1 is that the mass fraction of Sophora flavescens is 44%;

[0039] The only difference between Example 8 and Example 1 is that the mass fraction of Coptis chinensis is 28%;

[0040] The only difference between Example 9 and Example 1 is that the mass fraction of Coptis chinensis is 40%;

[0041] The only difference between Example 10 and Example 1 is that the mass fraction of the nourishing extract is 40%;

[0042] The only difference between Example 11 and Example 1 is that the mass fraction of the nourishing extract is 53%;

[0043] The only difference between Example 12 and Example 1 is that the mass fraction of the antibacterial extract is 27%;

[0044] The only difference between Example 13 and Example 1 is that the mass fraction of the antibacterial extract is 38%;

[0045] The only difference between Example 14 and Example 1 is that the mass fraction of nisin is 2%;

[0046] The only difference between Example 15 and Example 1 is that the mass fraction of nisin is 4%.

[0047] Comparative Examples 1-27:

[0048] The difference between Comparative Example 1 and Example 1 is that the mass fraction of Radix Rehmanniae Preparata is 30%;

[0049] The difference between Comparative Example 2 and Example 1 is that the mass fraction of Rehmannia glutinosa is 70%;

[0050] The difference between Comparative Example 3 and Example 1 is that the mass fraction of Salvia miltiorrhiza is 10%;

[0051] The difference between Comparative Example 4 and Example 1 is that the mass fraction of Salvia miltiorrhiza is 45%;

[0052] The only difference between Comparative Example 5 and Example 1 is that the mass fraction of Sophora flavescens is 15%;

[0053] The only difference between Comparative Example 6 and Example 1 is that the mass fraction of Sophora flavescens is 60%;

[0054] The difference between Comparative Example 7 and Example 1 is that the mass fraction of Coptis chinensis is 14%;

[0055] The only difference between Comparative Example 8 and Example 1 is that the mass fraction of Coptis chinensis is 60%;

[0056] The only difference between Comparative Example 9 and Example 1 is that the mass fraction of the nourishing extract is 20%;

[0057] The only difference between Comparative Example 10 and Example 1 is that the mass fraction of the nourishing extract is 65%;

[0058] The only difference between Comparative Example 11 and Example 1 is that the mass fraction of the antibacterial extract is 13%;

[0059] The only difference between Comparative Example 12 and Example 1 is that the mass fraction of the antibacterial extract is 50%;

[0060] The only difference between Comparative Example 13 and Example 1 is that the mass fraction of nisin is 1%;

[0061] The only difference between Comparative Example 14 and Example 1 is that the mass fraction of nisin is 8%;

[0062] The only difference between Comparative Example 15 and Example 1 is that in the preparation method of the nourishing extract, ethanol is immersed at 56°C;

[0063] The only difference between Comparative Example 16 and Example 1 is that in the preparation method of the antibacterial extract, ethanol is immersed at 78°C;

[0064] The difference between Comparative Example 17 and Example 1 is that in the preparation method of the nourishing extract, the temperature of the reduced pressure distillation is 60°C, and in the preparation method of the antibacterial extract, the temperature of the reduced pressure distillation is 69°C;

[0065] The difference between Comparative Example 18 and Example 1 is that Radix Rehmanniae Preparata is replaced with Radix Salviae Miltiorrhizae of equal quality;

[0066] The difference between Comparative Example 19 and Example 1 is that Salvia miltiorrhiza is replaced with Rehmannia glutinosa of equal quality;

[0067] The difference between Comparative Example 20 and Example 1 is that Sophora flavescens is replaced with Coptis chinensis of equal mass;

[0068] The difference between Comparative Example 21 and Example 1 is that Rhizoma Coptidis is replaced with Sophora flavescens of equal mass;

[0069] The difference between Comparative Example 22 and Example 1 is that the nourishing extract is replaced with an equal mass of the antibacterial extract and nisin in the proportion of Example 1;

[0070] The difference between Comparative Example 23 and Example 1 is that the antibacterial extract is replaced with an equal mass of the nutrient extract and nisin in the proportion of Example 1;

[0071] The difference between Comparative Example 24 and Example 1 is that nisin is replaced by the nutrient extract and the antibacterial extract of equal mass in the proportion of Example 1;

[0072] The difference between Comparative Example 25 and Example 1 is that Radix Rehmanniae Preparata is replaced with Fructus Corni of equal mass;

[0073] The difference between Comparative Example 26 and Example 1 is that Rhizoma Coptidis is replaced with Flos Lonicerae of equal mass;

[0074] The only difference between Comparative Example 27 and Example 1 is that the vaginal antibacterial liquid is replaced with pure water of equal mass.

[0075] The same number of female volunteers aged 30 with bacterial vaginosis as in the example of the present invention were recruited. The sampling densities of the two harmful bacteria in their vaginas were basically the same in this experiment: the density of Staphylococcus aureus was 25×10 6 cfu / g and the density of E. coli was 60×10 6 cfu / g; the above volunteers were irrigated with a type II telescopic 200ml medical vaginal irrigator, and 200ml of the vaginal antibacterial liquid of each example was irrigated. After 20 minutes, the liquid was discharged and the vulva was cleaned. This was repeated once a day. After one week, 3g of secretions were sampled from the vaginal wall using a sanitary tampon. The density of Escherichia coli was detected by referring to part 7.3 of standard SN / T1607-2017, and the density of Staphylococcus aureus was detected by referring to the second method of Staphylococcus aureus Baird-Parker plate count in standard GB4789.10-2010.

[0076] The results of the test are shown in Table 1. The density units of Staphylococcus aureus and Escherichia coli are 10 6 cfu / g, keep integer.

[0077] Table 1: Test results of Staphylococcus aureus and Escherichia coli density of volunteers corresponding to each example.

[0078]

[0079] It can be seen from the data in Table 1 that, compared with other examples, the vaginal antibacterial liquid prepared by the embodiments of the present invention, especially the vaginal antibacterial liquid and the preparation method thereof of Embodiment 1 of the present invention, has a low density of Staphylococcus aureus and Escherichia coli at the same dosage of the liquid; that is, the natural ingredients of the vaginal antibacterial liquid obtained by the scheme of the present invention can effectively inhibit and kill vaginal pathogens such as Staphylococcus aureus and Escherichia coli.

[0080] It should be noted that the present invention uses Staphylococcus aureus and Escherichia coli to represent bacterial vaginal pathogens. The above process parameters and effects are nonlinearly correlated, and the matching conditions with other components and processes of the present invention should be considered. The above description is only an embodiment of the present invention and is not intended to limit the present invention; for those skilled in the art, the present invention may have various changes and variations; any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of the claims of the present invention.

Claims

1. A method for preparing a vaginal antibacterial solution, characterized in that: The preparation method of the vaginal antibacterial liquid comprises the following steps: Step 1: Take 42-58% of Rehmannia glutinosa, 21-30% of Salvia miltiorrhiza and pure water to make up the rest, cut them into pieces and pass through a 50-mesh sieve, add 80% ethanol with a mass of 10 times the mass of the Rehmannia glutinosa and Salvia miltiorrhiza, immerse them at 78°C for 24 hours, add them into a flash extractor with a speed of 8000 r / min, extract for 30 seconds and filter to obtain a coarse filtrate extract; add 0.2 times the mass of pure water to the coarse filtrate extract and distill it under reduced pressure at 69°C and a vacuum degree of 800 mbar for 20 minutes, collect the remaining liquid, add 88% lactic acid to adjust the pH to 3.5 to obtain a nourishing extract; Step 2: Take 30-44% of Sophora flavescens by mass, 28-40% of Coptis chinensis by mass and make up the rest with pure water, cut them into pieces and pass through a 50-mesh sieve, add 80% ethanol with a mass of 10 times the mass of the Sophora flavescens and the Coptis chinensis, immerse them at 56°C for 24 hours, add them into a flash extractor with a speed of 5000r / min, extract for 30s and filter to obtain a coarse filtrate extract; add 0.2 times the mass of pure water to the coarse filtrate extract and distill it under reduced pressure at 60°C and 800mbar for 20min, collect the remaining liquid, add 88% lactic acid to adjust the pH to 3.5 to obtain an antibacterial extract; Step 3: Mix 40-53% of the nourishing extract, 27-38% of the antibacterial extract, 2-4% of nisin and pure water to make up the rest to obtain a vaginal antibacterial solution.

2. The method for preparing the vaginal antibacterial solution according to claim 1, characterized in that: The mass fraction of the Radix Rehmanniae Preparata is 51%.

3. The method for preparing the vaginal antibacterial solution according to claim 1, characterized in that: The mass fraction of the salvia miltiorrhiza is 26%.

4. The method for preparing the vaginal antibacterial solution according to claim 1, characterized in that: The mass fraction of the Sophora flavescens is 38%.

5. The method for preparing the vaginal antibacterial liquid according to claim 1, characterized in that: The mass fraction of the coptis root is 35%.

6. The method for preparing the vaginal antibacterial liquid according to claim 1, characterized in that: The mass fraction of the nourishing extract is 47%.

7. The method for preparing the vaginal antibacterial liquid according to claim 1, characterized in that: The mass fraction of the antibacterial extract is 32%.

8. The method for preparing the vaginal antibacterial liquid according to claim 1, characterized in that: The mass fraction of the nisin is 3%.

9. A vaginal antibacterial liquid prepared by the method for preparing a vaginal antibacterial liquid according to any one of claims 1 to 8, characterized in that: The vaginal antibacterial liquid comprises: 40-53% of the nourishing extract by mass fraction, 27-38% of the antibacterial extract by mass fraction, 2-4% of the nisin by mass fraction and pure water to make up the rest by mass fraction.

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