Traditional Chinese medicine composition, pharmaceutical preparation and use for treating and / or preventing sepsis
By combining honeysuckle, forsythia, red peony root, salvia miltiorrhiza, peony bark, and licorice, the treatment of sepsis has been solved, achieving reduced mortality, prolonged survival time, and protection against organ damage caused by sepsis.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2024-07-25
- Publication Date
- 2026-04-07
AI Technical Summary
Current technology lacks effective drugs for treating sepsis, the overuse of antibiotics has led to increased bacterial resistance, supportive therapy cannot cure the disease, and finding effective drugs has become a major challenge.
The herbal composition consists of honeysuckle, forsythia, red peony root, salvia miltiorrhiza, peony bark, and licorice. It reduces sepsis damage by inhibiting the expression of inflammatory factors and protecting organ function.
It significantly reduced the mortality rate of septic mice, prolonged their survival time, reduced the expression of inflammatory factors, alleviated organ damage, and protected the function of the lungs, liver, and kidneys.
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Figure CN118831115B_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The present disclosure relates generally to the field of traditional Chinese medicine, and in particular to a traditional Chinese medicine composition, a pharmaceutical preparation and uses thereof for treating and / or preventing sepsis. BACKGROUND
[0002] Sepsis is defined as a syndrome of organ dysfunction caused by a dysregulated host response to infection, characterized by the presence of high levels of pro-inflammatory cytokines (such as IL-1β, TNF-α and IL-6) that drive excessive inflammatory responses, leading to multiple organ failure and ultimately death. Sepsis is characterized by high mortality and difficulty in treatment, and is a common complication of critically ill patients in clinical departments, especially in ICU, and is also the main cause of death of patients in intensive care units.
[0003] Currently, the treatment of sepsis mainly relies on antibacterial drugs and supportive therapies such as fluid resuscitation and mechanical ventilation. However, the abuse of antibacterial drugs leads to the increase of bacterial drug resistance, and supportive therapies cannot cure the disease. Although there are new breakthroughs in the study of sepsis, the exploration of drug treatment for regulating sepsis response is not successful, and finding effective drugs for treating sepsis has become a major proposition in the medical field. SUMMARY
[0004] Based on the above background, the present disclosure provides a traditional Chinese medicine composition for treating and / or preventing sepsis, wherein the traditional Chinese medicine composition is made of the following traditional Chinese medicine raw materials in parts by weight: honeysuckle 10-20 parts, forsythia 10-20 parts, red peony root 10-20 parts, salvia miltiorrhiza 7-13 parts, tree peony bark 7-13 parts, and licorice 4-8 parts.
[0005] In another aspect, the present disclosure also provides a pharmaceutical preparation, wherein the pharmaceutical preparation comprises the traditional Chinese medicine composition described herein or an extract thereof.
[0006] In another aspect, the present disclosure also provides the use of the traditional Chinese medicine composition described herein or the pharmaceutical preparation described herein in the preparation of a medicament for treating and / or preventing sepsis.
[0007] The present disclosure uses a cecal ligation puncture (CLP) induced sepsis mouse model, and finds that the traditional Chinese medicine composition of the present disclosure can significantly reduce the mortality rate of sepsis mice, prolong the survival time of sepsis mice, reduce the expression level of inflammatory factors TNF-α, IL-6 and IL-1β in the serum of sepsis mice, and reduce the damage degree of target organs such as lung, liver and kidney. By inhibiting the levels of MPO, ALT, AST and CRE, the organ function of lung, liver and kidney is protected. These contents show that the traditional Chinese medicine composition of the present disclosure has good effect for treating and / or preventing sepsis, and can be used for preparing a medicament for treating and / or preventing sepsis.
[0008] Other features and advantages of the present application will be set forth in the following specification, and in part will be apparent from the description, or can be learned by practice of the application. Other advantages of the application will be realized and attained by the solution described in the specification and claims. BRIEF DESCRIPTION OF DRAWINGS
[0009] The accompanying drawings are included to provide a further understanding of the application and are incorporated in and constitute a part of this specification, illustrate embodiments of the application and together with the description serve to explain the principles of the application, but are not intended to limit the application.
[0010] Figure 1 Figure 1 shows the effect of the traditional Chinese medicine composition in one embodiment of the present application on the survival time of CLP-induced sepsis mice; wherein n = 10, compared with the sham operation group, ###p < 0.001; compared with the model group, *p < 0.05.
[0011] Figure 2 Figure 2 shows the effect of the traditional Chinese medicine composition in one embodiment of the present application on the levels of pro-inflammatory cytokines in the serum of CLP-induced sepsis mice. In Figure 2 Figure 2 shows the effect of the traditional Chinese medicine composition in one embodiment of the present application on the levels of pro-inflammatory cytokines in the serum of CLP-induced sepsis mice. In
[0012] Figure 3 Figure 3 shows the effect of the traditional Chinese medicine composition in one embodiment of the present application on the lung, liver and kidney tissue damage of sepsis-mediated mice.
[0013] Figure 4 Figure 4 shows the protective effect of the traditional Chinese medicine composition in one embodiment of the present application on the multiple organ damage of CLP-induced sepsis mice. In Figure 4A, the effect of the traditional Chinese medicine composition on the expression of myeloperoxidase (MPO) in the lung tissue of sepsis mice, wherein, compared with the sham operation group, ###p<0.0001; compared with the model group, ***p<0.0001. B, the effect of the traditional Chinese medicine composition on the activity of alanine aminotransferase (ALT) in the liver tissue of sepsis mice, wherein, compared with the sham operation group, ###p<0.001; compared with the model group, **p<0.01, ***p<0.001, ***p<0.0001. C, the effect of the traditional Chinese medicine composition on the activity of aspartate aminotransferase (AST) in the liver tissue of sepsis mice, wherein, compared with the sham operation group, ###p<0.001; compared with the model group, **p<0.01, ***p<0.0001. D, the effect of the traditional Chinese medicine composition on the content of creatinine (CRE) in the serum of sepsis mice, wherein, compared with the sham operation group, ###p<0.0001; compared with the model group, **p<0.01, ***p<0.001. DETAILED DESCRIPTION
[0014] Unless otherwise indicated, the technical and scientific terms used herein have the same meaning as those which will be understood by those skilled in the art to which this application pertains. When referring to a quantity, concentration, or other value or parameter, the use of the term "about" in connection with a range, preferably a range that is less than 10%, preferably less than 1%, and more preferably less than 0.1%, of the maximum limit of a stated range, is meant to refer to a range that includes the end points of the range and all integers and fractions (decimals) within the range.
[0015] The terms "about" and "approximately" when used in connection with a numerical value, generally mean a value of the numerical value and all values of the numerical value that are within experimental error (e.g., within 95% confidence interval of the mean) or within ±10% of the stated value, or within a broader range.
[0016] The expressions "comprising" or "comprise," and grammatical variants thereof, are open-ended, and do not exclude additional, unrecited elements or steps. The expression "consisting of" excludes any element, step, or ingredient not specified. The expression "consisting essentially of" means that the range is limited to the specified elements, steps or ingredients, plus an optional additional element, step, or ingredient that does not materially affect the basic and novel characteristics of the claimed subject matter. It is understood that the expression "comprising" encompasses the expressions "consisting of" and "consisting essentially of."
[0017] The expressions "at least one" or "one or more" mean 1, 2, 3, 4, 5, 6, 7, 8, 9, or more.
[0018] In recent years, traditional Chinese medicine has made some progress in the comprehensive regulation of complex diseases from multiple targets, multiple pathways and multiple mechanisms, and its effectiveness and safety in disease treatment are increasingly recognized. In the clinical treatment of sepsis in China, the use of traditional Chinese medicine has also achieved certain clinical efficacy. Traditional Chinese medicine theory believes that the internal cause of sepsis is deficiency of vital qi, and the external cause is invasion of toxic evil. The pathogenesis is deficiency of vital qi and damage by toxic evil, toxic heat, blood stasis, phlegm turbidity and stasis in collaterals, disorder of qi movement, and dysfunction of zang-fu organs. Clinically, the treatment is based on the principle of clearing heat and resolving toxins, promoting blood circulation and removing blood stasis, and tonifying deficiency and strengthening vital qi. According to the specific syndrome type of the patient, the medicine is used to remove the pathological products accumulated after the damage of collaterals, and restore the overall environment of the body with harmonious ying and wei and smooth qi and blood. Therefore, in the face of the complex pathogenesis of sepsis, the development of traditional Chinese medicine compositions for the treatment of sepsis has good application prospects. The traditional Chinese medicine composition of the present application is sweet and cold, and belongs to the lung, heart and stomach meridians, and has the effects of clearing heat and resolving toxins, and cooling and dispersing wind-heat. Forsythia is bitter and slightly cold, and belongs to the lung, heart and gallbladder meridians, and has the effects of clearing heat and resolving toxins, and resolving abscesses and resolving knots. Honeysuckle and forsythia are compatible with the effects of clearing heat and resolving toxins, and resolving abscesses. Red peony root is bitter and cold, and has the effects of clearing heat and cooling blood, and resolving stasis and relieving pain. Peony bark is bitter and pungent, and slightly cold, and has the effects of clearing heat and cooling blood, and promoting blood circulation. Salvia is bitter and slightly cold, and has the effects of promoting blood circulation and removing blood stasis, cooling blood and resolving abscesses, and nourishing blood and calming the nerves. Red peony root, salvia and peony bark are compatible with the effects of clearing heat and cooling blood, and promoting blood circulation and removing blood stasis. Licorice regulates all medicines and clears heat and resolves toxins. The overall prescription has the effects of clearing heat and resolving toxins, and resolving abscesses, and is suitable for the treatment of symptoms such as fever, rash, abscesses and sores caused by wind-heat invading the blood.
[0019] The present application provides a traditional Chinese medicine composition for treating and / or preventing sepsis, wherein the traditional Chinese medicine composition comprises, by weight, honeysuckle 10-20 parts, forsythia 10-20 parts, red peony root 10-20 parts, salvia 7-13 parts, peony bark 7-13 parts and licorice 4-8 parts.
[0020] In some embodiments, the traditional Chinese medicine composition is made of, by weight, honeysuckle 10-20 parts, forsythia 10-20 parts, red peony root 10-20 parts, salvia 7-13 parts, peony bark 7-13 parts and licorice 4-8 parts.
[0021] In some embodiments, the traditional Chinese medicine composition is made of, by weight, honeysuckle 10-20 parts, forsythia 10-20 parts, red peony root 10-20 parts, salvia 7-13 parts, peony bark 7-13 parts and licorice 4-8 parts.
[0022] In some embodiments, the traditional Chinese medicine composition is made of, by weight, honeysuckle 10-20 parts, forsythia 10-20 parts, red peony root 10-20 parts, salvia 7-13 parts, peony bark 7-13 parts and licorice 4-8 parts.
[0023] In another aspect, the present application also provides a pharmaceutical preparation, wherein the pharmaceutical preparation comprises the traditional Chinese medicine composition described herein or an extract thereof.
[0024] In some embodiments, the extract is a water extract or an alcohol extract of the traditional Chinese medicine composition described herein.
[0025] In some embodiments, the pharmaceutical preparation further comprises a pharmaceutically acceptable excipient.
[0026] In some embodiments, the excipient is selected from one or more of a pharmaceutically acceptable solvent, a solubilizer, a co-solvent, an emulsifier, a coloring agent, a binder, a disintegrant, a filler, a lubricant, a wetting agent, an osmotic pressure adjusting agent, a stabilizer, a flavoring agent, a preservative, a suspending agent, a coating material, an integrating agent, a penetration enhancer, a pH adjusting agent, a buffer, a plasticizer, a surfactant, a thickening agent, a complexing agent, a humectant, a flocculating agent and a deflocculating agent, a filtering aid, a release retardant, a high molecular backbone material, and a film-forming material.
[0027] In some embodiments, the dosage form of the pharmaceutical preparation is a tablet, a capsule, a powder, a pill, a granule, an oral solution, a syrup, a decoction, a suppository, a gel, a spray, or an injection. In some embodiments, the dosage form of the pharmaceutical preparation in the present application includes a capsule, a tablet, a pill, a liquid, a powder, a granule, a fine granule, a film-coated agent, a pill, a lozenge, a sublingual agent, a gelatin solution, a buccal preparation, a paste, a syrup, a suspension, an elixir, an emulsion, a coating agent, an ointment, a plaster, a cataplasm, a transdermal preparation, a lotion, an inhalant, an aerosol, an injection, and a suppository.
[0028] In some embodiments, the pharmaceutical preparation is administered orally, intravenously, intradermally, transdermally, intrathecally, intra-arterially, intraperitoneally, intranasally, intravaginally, intrarectally, intravesically, intratumorally, topically, intramuscularly, subcutaneously, mucosally, by inhalation, by injection, by infusion, or any combination thereof.
[0029] In another aspect, the present application also provides the use of the traditional Chinese medicine composition described herein or the pharmaceutical preparation described herein in the preparation of a medicament for treating and / or preventing sepsis.
[0030] In another aspect, the present application also provides a method for treating and / or preventing sepsis, the method comprising: administering to a subject the traditional Chinese medicine composition described herein or the pharmaceutical preparation described herein.
[0031] In some implementations, the subjects are humans, rats, mice, cats, dogs, horses, sheep, cows, and monkeys. Illustrative examples of subjects in some implementations include primates, particularly humans; companion animals such as cats and dogs and similar animals; working animals such as horses, donkeys, and similar animals; livestock animals such as sheep, cattle, goats, pigs, and similar animals; laboratory test animals such as rabbits, mice, rats, guinea pigs, hamsters, and similar animals; and captive wild animals such as captive wild animals in zoos and wildlife parks, deer, dingoes, and similar animals. In some implementations, the subjects are humans.
[0032] This application describes several embodiments, but these descriptions are exemplary and not restrictive, and it will be apparent to those skilled in the art that many more embodiments and implementations are possible within the scope of the embodiments described herein. Although many possible combinations of features are shown in the accompanying drawings and discussed in the detailed description, many other combinations of the disclosed features are also possible. Unless specifically limited, any feature of any embodiment may be used in combination with any other feature in any other embodiment, or may replace any other feature in any other embodiment.
[0033] This application includes and contemplates combinations of features known to those skilled in the art. The embodiments and features disclosed in this application can also be combined with any conventional features to form a unique inventive scheme as defined by the claims. Any feature of any embodiment can also be combined with features from other inventive schemes to form another unique inventive scheme as defined by the claims. Therefore, it should be understood that any feature shown and / or discussed in this application can be implemented individually or in any suitable combination. Therefore, the embodiments are not limited except by the limitations imposed by the appended claims and their equivalents. Furthermore, various modifications and changes can be made within the scope of the appended claims.
[0034] Furthermore, in describing representative embodiments, the specification may have presented methods and / or processes as a specific sequence of steps. However, the method or process should not be limited to the specific order of steps described herein, to the extent that it does not depend on such a specific order. As will be understood by those skilled in the art, other sequences of steps are also possible. Therefore, the specific order of steps set forth in the specification should not be construed as a limitation of the claims. Moreover, the claims concerning the method and / or process should not be limited to the steps performed in the written order, and those skilled in the art will readily understand that these orders can be varied and still remain within the spirit and scope of the embodiments of this application.
[0035] Experimental methods in the following embodiments without specific conditions are generally determined according to national standards. Experimental materials in the following embodiments without specified sources are all commercially available raw materials. The equipment used in each step of the following embodiments is conventional equipment. If there is no corresponding national standard, then generally accepted international standards, conventional conditions, or conditions recommended by the manufacturer are followed. Unless otherwise defined or stated, all technical and scientific terms used in this application have the same meaning as those skilled in the art. Furthermore, any methods and materials similar or equivalent to those described herein may be applied to the methods of this application.
[0036] Example
[0037] The materials used in the embodiments are shown below:
[0038] 1. Materials, reagents,
[0039] Sterile syringes, surgical scissors, forceps, medical tape, gauze, 4-0 surgical sutures, surgical drapes, 21-gauge puncture needles, iodine for disinfection, and physiological saline. Universal tissue fixative, sodium pentobarbital, TNF-α ELISA kit, IL-6 ELISA kit, IL-1β ELISA kit, ALT / GPT test kit, AST / GOT test kit, (MPO) ELISA kit, and (CRE) content kit (sarcosine oxidase method).
[0040] 2. Instruments
[0041] Autoclave, constant temperature water bath, ELISA reader
[0042] Example 1. Preparation of Traditional Chinese Medicine Composition
[0043] The traditional Chinese medicine composition used in this application (honeysuckle 15g, forsythia 15g, red peony root 15g, salvia miltiorrhiza 10g, peony bark 10g, licorice root 6g) is prepared by combining the formula granules of each herb. Referring to the granule production standard, the dosage of the raw herbs was converted into the granule dosage (table), and all granules were then thoroughly mixed to obtain the traditional Chinese medicine composition used in this application. In animal experiments, the traditional Chinese medicine composition was dissolved in sterile physiological saline to prepare a composition of a certain concentration. (The dosage was calculated based on the amount of raw herbs.)
[0044]
[0045] Example 2. Construction, grouping, and administration of a CLP-induced sepsis mouse model
[0046] laboratory animals
[0047] C57BL / 6 mice (weighing 20±2g) were purchased from Spifort (Beijing) Biotechnology Co., Ltd. All mice were housed together in pathogen-free cages with free access to food and water, and kept in a quiet environment at 22±2℃ for one week under a 12-hour light / dark cycle.
[0048] Model construction
[0049] Preoperatively, all mice were fasted for 12 hours but allowed free access to water. Mice were anesthetized with 1% sodium pentobarbital and fixed to a sterile operating table. Under sterile conditions, a 1cm incision was made along the midline of the abdomen. The epidermis and muscle layers were dissected layer by layer to expose the cecum. Feces were gently squeezed to the distal end of the cecum, and then the cecum was ligated with surgical sutures 1cm distal to the cecum. After ligation, a 21-gauge needle was used to make a transverse puncture about 0.5cm above the blind end of the cecum. The needle was passed back and forth twice, and a small amount of intestinal contents were squeezed out through the puncture site. The cecum was then returned to the abdominal cavity, and the muscle and skin layers were sutured layer by layer. The sham-operated group underwent the same surgical procedure but without CLP. After surgery, each mouse was subcutaneously injected with 5mL / kg of preheated saline for fluid resuscitation and returned to its cage for free access to food and water.
[0050] Grouping and processing
[0051] After one week of acclimatization, mice were randomly divided into five groups as follows: sham-operated group (mice underwent sham surgery without drug treatment); model group (mice underwent CLP surgery without drug treatment); low-dose traditional Chinese medicine (TCM) combination group (mice underwent CLP surgery and were treated with 10.8 g / kg TCM combination); high-dose TCM combination group (mice underwent CLP surgery and were treated with 21.6 g / kg TCM combination); and positive control group (mice underwent CLP surgery and were treated with 4.6 g / kg Huanglian Jiedu Decoction). Before CLP surgery, the low-dose TCM combination group received 0.2 mL of the 10.8 g / kg TCM combination via gavage; the high-dose TCM combination group received 0.2 mL of the 21.6 g / kg TCM combination via gavage; and the positive control group (4.6 g / kg) received 0.2 mL of the 4.6 g / kg Huanglian Jiedu Decoction via gavage. The sham-operated and model groups received the same volume of physiological saline via gavage. Administer the drug by gavage once every 24 hours for 5 consecutive days. On the 6th day, establish the model according to the above model construction method. After the model is established, the mice will be lethargic, curled up in a corner, and unresponsive after recovery. This indicates that the model has been successfully established. Then, administer the drug by gavage.
[0052] Example 3. Evaluation of the effect of the traditional Chinese medicine composition on the survival rate of CLP-induced septic mice within 7 days.
[0053] As in Example 2, before modeling, the mice were administered the drug via gavage once every 24 hours for 5 consecutive days. Modeling was performed on the 6th day. After the mice recovered and were treated with the drug via gavage, 10 mice were randomly selected from each group to assess their survival. The mice were then continued to be administered the drug via gavage once every 24 hours. Mice mortality was recorded at the same time each day until the 7th day after modeling was completed. During this period, all mice had free access to water and food.
[0054] Experimental results:
[0055] Depend on Figure 1 It was found that mice in the sham-operated group did not die within 7 days after surgery, while all mice in the model group died on day 4, showing a significantly lower survival rate compared to the sham-operated group. After intervention with the traditional Chinese medicine composition, the survival rates of septic mice in the low-dose group (10.8 g / kg) and the high-dose group (21.6 g / kg) were significantly improved compared to the model group. This traditional Chinese medicine composition treatment reduced the mortality rate of CLP-induced septic mice.
[0056] Example 4. Determination of the effect of a traditional Chinese medicine composition on the serum levels of pro-inflammatory cytokines in CLP-induced septic mice.
[0057] As in Example 2, before modeling, the drug was administered by gavage once every 24 hours for 5 consecutive days. Modeling was performed on the 6th day. After the mice woke up, they were treated by gavage. Six mice were randomly selected from each group. 24 hours after modeling, the mice were enucleated to collect blood. The mouse blood was centrifuged and the serum was collected. The levels of inflammatory factors TNF-α, IL-6 and IL-1β in the serum were detected using an ELISA kit.
[0058] Experimental results:
[0059] Depend on Figure 2 It was found that, compared with the sham-operated group, the serum concentrations of TNF-α, IL-6, and IL-1β in the model group were significantly increased. In mice treated with the traditional Chinese medicine composition (10.8 g / kg and 21.6 g / kg), the accumulation of the above-mentioned inflammatory factors was significantly reduced, and the traditional Chinese medicine composition could alleviate the inflammatory response caused by sepsis.
[0060] Example 5. HE staining was used to detect lung, liver, and kidney tissue damage in mice of each group.
[0061] In Example 4, after blood was collected from the mouse eyeballs, the mice were euthanized by cervical dislocation. Lung, liver, and kidney tissues were removed from the mice. A portion of the tissues was placed in 4% paraformaldehyde tissue fixative and stained with hematoxylin and eosin (HE) to detect the degree of pathological damage.
[0062] Experimental results:
[0063] Depend on Figure 3 It can be seen that, compared with the sham-operated group, the model group mice showed obvious pathological changes in all organs:
[0064] In lung tissue, the sham-operated group showed no obvious lesions; the model group showed obvious lesions, with alveoli of varying sizes, alveolar structure destruction, alveolar collapse, some alveoli merging and expanding, alveolar walls thickening, and obvious inflammatory cell and erythrocyte infiltration. When the Chinese herbal medicine combination was used to treat septic mice, the occurrence of inflammatory cell and erythrocyte infiltration and alveolar wall thickening was reduced, and some alveoli returned to normal morphology. Compared with the two dosage groups, the high-dose group had a more significant protective effect against lung injury in septic mice.
[0065] In liver tissue, the sham-operated group showed no obvious lesions; the model group showed obvious lesions, with indistinct liver lobule structure, disordered hepatic cords, narrowing or even disappearance of hepatic sinusoids, hepatocyte necrosis, partial hepatocyte nucleus rupture and dissolution, and a small amount of inflammatory cell infiltration. When the traditional Chinese medicine composition was used to treat septic mice, the liver tissue damage was significantly improved, and compared with the two dosage groups, the high-dose group had a more significant protective effect on liver damage in septic mice.
[0066] In the kidney tissue, the sham-operated group showed no obvious lesions; the model group showed obvious lesions, with irregular glomerular outlines, narrowing or disappearance of the Bowman's capsule, adhesion between the glomeruli and Bowman's capsule, and atrophy of the renal tubules with unclear structures. When the septic mice were treated with the traditional Chinese medicine composition, the structures of the glomeruli and renal tubules gradually became clear, and the kidney tissue damage was significantly improved. Compared with the two dosage groups, the high-dose group had a more significant protective effect against kidney damage in septic mice.
[0067] The above results indicate that the damage to the lungs, liver, and kidneys in mice treated with the traditional Chinese medicine composition was significantly improved, demonstrating that the traditional Chinese medicine composition can protect against multi-organ damage caused by sepsis.
[0068] Example 6. Evaluation of the protective effect of the traditional Chinese medicine composition against CLP-induced multi-organ damage in CLP-induced septic mice by detecting relevant indicators.
[0069] In Example 5, another portion of the mouse lung and liver tissue was placed in cryovials and stored at -80°C. Tissue homogenization was performed before testing, and proteins in the tissue were quantified using the BCA method and then normalized. Lung and liver function-related indicators were detected using myeloperoxidase (MPO), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) assay kits, respectively. Kidney function was assessed by detecting the creatinine (CRE) content in the serum of the mice from Example 4.
[0070] Experimental results:
[0071] Depend on Figure 4It was found that in the model group, the expression levels of MPO in lung tissue, ALT and AST in liver tissue, and CRE (renal function marker) in serum were significantly increased. Conversely, treatment with the traditional Chinese medicine composition (10.8 g / kg and 21.6 g / kg) significantly reduced the expression of the above markers. The results indicate that the traditional Chinese medicine composition can alleviate CLP-induced organ damage in the lungs, liver, and kidneys of CLP-induced septic mice and protect organ function.
Claims
1. A traditional Chinese medicine composition for treating and / or preventing sepsis, wherein, The traditional Chinese medicine composition is made from the following raw materials by weight: 10-20 parts of honeysuckle, 10-20 parts of forsythia, 10-20 parts of red peony root, 7-13 parts of salvia miltiorrhiza, 7-13 parts of peony bark, and 4-8 parts of licorice.
2. The traditional Chinese medicine composition according to claim 1, wherein, The traditional Chinese medicine composition is made from the following raw materials by weight: 12-18 parts of honeysuckle, 12-18 parts of forsythia, 12-18 parts of red peony root, 8-12 parts of salvia miltiorrhiza, 8-12 parts of peony bark, and 5-7 parts of licorice.
3. The traditional Chinese medicine composition according to claim 2, wherein, The traditional Chinese medicine composition is made from the following raw materials by weight: 15 parts honeysuckle, 15 parts forsythia, 15 parts red peony root, 10 parts salvia miltiorrhiza, 10 parts peony bark, and 6 parts licorice.
4. A pharmaceutical preparation, wherein, The pharmaceutical preparation comprises the traditional Chinese medicine composition or extract thereof as described in any one of claims 1-3.
5. The pharmaceutical preparation according to claim 4, wherein, The extract is an aqueous or alcoholic extract of the traditional Chinese medicine composition.
6. The pharmaceutical preparation according to claim 4, wherein, The pharmaceutical preparation also contains pharmaceutically acceptable excipients.
7. The pharmaceutical preparation according to claim 4, wherein, The dosage form of the pharmaceutical preparation is tablets, capsules, powders, pills, granules, oral liquids, syrups, decoctions, suppositories, gels, sprays, or injections.
8. Use of the traditional Chinese medicine composition of any one of claims 1-3 or the pharmaceutical preparation of any one of claims 4-7 in the preparation of a medicament for the treatment and / or prevention of sepsis.