A Chinese medicine composition for treating influenza and its preparation method

Abstract

The invention discloses a Chinese medicine composition for treating influenza and a preparation method thereof, wherein the Chinese medicine composition is prepared from the following raw materials in parts by weight: 10-20 parts of white wormwood, 15-25 parts of walnut shells, 8-11 parts of kiwifruit roots, 6-10 parts of rue seeds, 5-7 parts of bran-fried yam, 3-5 parts of platycodon, 2-5 parts of atractylodes macrocephala, and 1-2 parts of liquorice. In the Chinese medicine composition of the present invention, white wormwood and walnut shells are monarch drugs, which can relieve inflammation, dissipate stasis, detoxify and relieve pain; kiwifruit root clears away heat and promotes diuresis, and rue seeds dissipate wind and dissipate heat, which are minister drugs; bran-fried yam relieves cough and eliminates phlegm, platycodon opens and promotes lung qi, and atractylodes macrocephala strengthens the spleen and replenishes qi, which are adjuvant drugs, and liquorice is used to reconcile various drugs, which is a guiding drug. It can effectively alleviate the symptoms of high fever, sore throat, cough and sputum caused by influenza.

Description

Technical Field

[0001] The present invention relates to the field of Chinese medicine preparations, and in particular to a Chinese medicine composition for treating influenza and a preparation method thereof. Background Art

[0002] Influenza is an acute respiratory infectious disease caused by influenza virus, which has the characteristics of short incubation period, rapid onset and strong infectivity. The main transmission routes of influenza are air droplet transmission and contact transmission. The typical symptoms of patients include body pain, rapid high fever, etc. At the same time, it can also induce pneumonia, acute bronchitis, encephalitis, pharyngitis and other diseases, which have a serious impact on the health of patients. Nowadays, in clinical practice, the treatment of influenza is mainly based on Western medicine diagnosis and treatment, and is treated by anti-influenza virus. However, there is no ideal specific drug at present, and Western medicine has large side effects. After taking the medicine, symptoms such as mental depression, drowsiness, and sleepiness often occur, which seriously affects the quality of life and work efficiency of patients. Therefore, through traditional Chinese medicine, it has become the common voice of doctors and patients to find new Chinese medicine preparations that can aromaticize turbidity, invigorate qi and detoxify, and improve immune function. At the same time, Chinese medicine is safe, non-toxic, and has few side effects. It has good effects in alleviating the course of the disease, reducing recurrence, maintaining stable condition and improving quality of life, and is easily accepted by patients. Summary of the invention

[0003] In order to overcome the deficiencies of the prior art, the present invention provides a Chinese medicine composition for treating influenza and a preparation method thereof.

[0004] The first object of the present invention is to provide a Chinese medicine composition for treating influenza.

[0005] The technical solution adopted in the first object of the present invention is:

[0006] A traditional Chinese medicine composition for treating influenza comprises the following raw materials in parts by weight: 10-20 parts of white wormwood, 15-25 parts of walnut shells, 8-11 parts of kiwi fruit roots, 6-10 parts of rutabaga seeds, 5-7 parts of fried yam with bran, 3-5 parts of platycodon, 2-5 parts of atractylodes macrocephala, and 1-2 parts of liquorice.

[0007] Furthermore, the Chinese medicine composition for treating influenza comprises the following raw materials in parts by weight: 15 parts of white wormwood, 22 parts of walnut shells, 10 parts of kiwi fruit roots, 8 parts of rutin seeds, 6 parts of fried yam with bran, 4 parts of platycodon, 4 parts of atractylodes, and 2 parts of licorice.

[0008] The second object of the present invention is to provide a method for preparing a Chinese medicine composition for treating influenza, which is simple and easy to operate.

[0009] The technical solution adopted in the second object of the present invention is:

[0010] A method for preparing a Chinese medicine composition for treating influenza comprises the following steps: weighing each raw medicine according to a ratio, crushing the raw medicine, placing the crushed raw medicine in an extraction tank, soaking the raw medicine in water, extracting the raw medicine at 45°C-65°C and a steam pressure of 0.045-0.048Mpa in the extraction tank, filtering, and concentrating the raw medicine to a total concentration of 1-2g / mL to obtain the Chinese medicine composition for treating influenza.

[0011] Furthermore, the weight ratio of the total weight of each raw material drug to water is 1:10-20.

[0012] Furthermore, the soaking time is 30-60 minutes, and the extraction time is 1-2 hours.

[0013] Furthermore, pharmaceutically acceptable excipients are added to the traditional Chinese medicine composition for treating influenza to prepare a corresponding dosage form.

[0014] Furthermore, the dosage form is an oral dosage form.

[0015] Furthermore, the oral dosage form is powder, oral liquid, granules, capsules, tablets or pills.

[0016] Furthermore, pharmaceutically acceptable excipients are added to the traditional Chinese medicine composition for treating influenza to prepare a corresponding dosage form, and the specific operation is: sucrose and dextrin are added to the concentrated medicinal liquid to prepare granules by a granulator; or the concentrated medicinal liquid is dried and crushed, and filled into capsules as capsule powder to prepare capsules; or sucrose and sodium benzoate are added to the concentrated medicinal liquid, stirred, and the pH value is adjusted to 7.0 to obtain an oral solution.

[0017] The pharmacological and medicinal properties of the raw materials used in the present invention are analyzed as follows:

[0018] Artemisia sphaerocephala: spicy and warm in nature; enters the liver, spleen and lung meridians; functions and indications: anti-inflammatory, dissipating blood stasis, and promoting qi.

[0019] Walnut shell: bitter and astringent, neutral in nature; enters the heart and liver meridians; functions and indications: stops bleeding, stops dysentery, disperses lumps and eliminates carbuncle, and stops itching.

[0020] Kiwi root: sweet, slightly bitter, cool in nature; enters the stomach and liver meridians; functions and indications: detoxification, clearing heat, diuresis, blood circulation, and swelling.

[0021] Rhizoma Rugosae: sweet in taste, slightly cold in nature; enters the heart, liver and spleen meridians; functions and indications: dispels wind and heat, nourishes the liver and improves eyesight.

[0022] Fried yam with bran: sweet in taste, neutral in nature; enters the spleen, lung and kidney meridians; functions and indications: invigorates qi and nourishes yin, tonifies the spleen and strengthens the stomach, benefits the kidney and consolidates essence, relieves cough and eliminates phlegm.

[0023] Platycodon grandiflorum: bitter and pungent in taste, neutral in nature; enters the lung meridian; functions and indications: invigorates the lungs, removes phlegm and relieves sore throat, and also discharges pus.

[0024] Atractylodes: sweet, slightly spicy; enters the spleen and stomach meridians; functions and indications: invigorates the spleen and replenishes Qi, protects the liver, promotes bile secretion, dries dampness and promotes diuresis, and stops sweating.

[0025] Licorice: sweet in taste, neutral in nature, enters the heart, lung, spleen, and stomach meridians; functions and indications: invigorating qi and tonifying the middle, removing phlegm and relieving cough, detoxifying, relieving pain, and anti-inflammatory.

[0026] The beneficial technical effects of the present invention are:

[0027] The invention provides a Chinese medicine composition for treating influenza, wherein white wormwood and walnut shell are both main drugs for anti-inflammatory, blood stasis, detoxification and pain relief; kiwi root clears heat and promotes diuresis, and radix rutaecarpa dispels wind and dissipates heat, and are both assistant drugs; bran-fried yam relieves cough and expectoration, platycodon opens and clears lung qi, and atractylodes macrocephala strengthens the spleen and replenishes qi, and are both adjuvant drugs; and liquorice is used to harmonize the various drugs, and is the guiding drug. The Chinese medicine composition of the invention can dispel blood stasis and wind, clear heat and relieve sore throat, and can be used to dispel evil and strengthen the body, and can effectively relieve the symptoms of high fever, sore throat, cough and sputum caused by influenza. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 A graph showing the expression level of inflammatory factor IL-1β in the serum of each group of virus-infected model mice 5 days after the Chinese medicine composition prepared in Examples 1-3 and Comparative Examples 1-4 was used to treat the virus-infected model mice;

[0029] Figure 2 A graph showing the expression level of inflammatory factor TNF-α in the serum of each group of virus-infected model mice after 5 days of treatment with the traditional Chinese medicine composition prepared in Examples 1-3 and Comparative Examples 1-4;

[0030] Figure 3 This is a graph showing the expression levels of the inflammatory factor IFN-γ in the serum of each group of virus-infected model mice 5 days after the Chinese medicine compositions prepared in Examples 1-3 and Comparative Examples 1-4 were used to treat the virus-infected model mice. DETAILED DESCRIPTION

[0031] The present invention will be described in further detail below in conjunction with the examples, but the embodiments of the present invention are not limited thereto. Unless otherwise specified, the reagents, methods and equipment used in the present invention are conventional reagents, methods and equipment in the art. The test methods for which specific experimental conditions are not specified in the following examples are usually carried out according to conventional experimental conditions or according to the experimental conditions recommended by the manufacturer. Unless otherwise specified, the reagents and raw materials used in the present invention can be obtained commercially.

[0032] Example 1

[0033] A traditional Chinese medicine composition for treating influenza comprises the following raw materials in parts by weight: 15 parts of white wormwood, 22 parts of walnut shells, 10 parts of kiwi fruit roots, 8 parts of rutabaga seeds, 6 parts of fried yam with bran, 4 parts of platycodon, 4 parts of atractylodes, and 2 parts of liquorice.

[0034] The preparation method of the above-mentioned Chinese medicine composition is as follows:

[0035] Weigh each raw material medicine according to the ratio, crush it, put the crushed raw material medicine into an extraction tank, add water to soak, the solid-liquid ratio is 1:10, soak for 30 minutes, perform steam distillation extraction at 65°C and a steam pressure of 0.045Mpa in the extraction tank for 1 hour, filter, and obtain a Chinese medicine composition extract; concentrate the Chinese medicine composition extract at 55°C to make the total concentration of each raw material medicine 2g / mL, and obtain the Chinese medicine composition for treating influenza.

[0036] Example 2

[0037] A traditional Chinese medicine composition for treating influenza comprises the following raw materials in parts by weight: 11 parts of white wormwood, 15 parts of walnut shells, 8 parts of kiwi fruit roots, 6 parts of rutabaga seeds, 5 parts of fried yam with bran, 3 parts of platycodon, 2 parts of atractylodes macrocephala, and 1 part of liquorice.

[0038] The preparation method of the above-mentioned Chinese medicine composition is as follows:

[0039] Weigh each raw material drug according to the ratio, crush it, put the crushed raw material drug into an extraction tank, add water to soak, the solid-liquid ratio is 1:10, soak for 60 minutes, at 65°C, keep the steam pressure in the extraction tank at 0.045Mpa, perform steam distillation extraction for 1.5 hours, filter, and obtain a Chinese medicine composition extract; concentrate the Chinese medicine composition extract at 55°C to make the total concentration of each raw material drug 1g / mL, and obtain the Chinese medicine composition for treating influenza.

[0040] Example 3

[0041] A traditional Chinese medicine composition for treating influenza comprises the following raw materials in parts by weight: 20 parts of white wormwood, 15 parts of walnut shells, 11 parts of kiwi fruit roots, 10 parts of rutabaga seeds, 6 parts of fried yam with bran, 5 parts of platycodon, 3 parts of atractylodes macrocephala, and 1 part of liquorice.

[0042] The preparation method of the above-mentioned Chinese medicine composition is as follows:

[0043] Weigh each raw material drug according to the ratio, crush it, put the crushed raw material drug into an extraction tank, add water to soak, the solid-liquid ratio is 1:10, soak for 45 minutes, at 65°C, keep the steam pressure in the extraction tank at 0.045Mpa, perform steam distillation extraction for 2 hours, filter, and obtain a Chinese medicine composition extract; concentrate the Chinese medicine composition extract at 55°C to make the total concentration of each raw material drug 2g / mL, and obtain the Chinese medicine composition for treating influenza.

[0044] Comparative Example 1

[0045] Comparative Example 1 provides a traditional Chinese medicine composition for treating influenza and a preparation method thereof, which is different from Example 1 in that Artemisia sphaerocephala is not added, and the rest is the same as Example 1.

[0046] Comparative Example 2

[0047] Comparative Example 2 provides a Chinese medicine composition for treating influenza and a preparation method thereof, which differs from Example 1 in that walnut shells are not added, and the rest are the same as Example 1.

[0048] Comparative Example 3

[0049] Comparative Example 3 provides a Chinese medicine composition for treating influenza and a preparation method thereof, which differs from Example 1 in that kiwifruit root is not added, the weight portion of Actinidia rutaecarpa is 18 portions, and the rest are the same as Example 1.

[0050] Comparative Example 4

[0051] Comparative Example 4 provides a Chinese medicine composition for treating influenza and a preparation method thereof, which differs from Example 1 in that no Rhizoma Cyperi is added, the weight portion of kiwifruit root is 18 portions, and the rest is the same as Example 1.

[0052] Test Example 1

[0053] The main purpose is to test the therapeutic effect of the Chinese medicine composition of the present invention on the lung function of influenza virus model mice.

[0054] (1) Test materials and drugs:

[0055] The Chinese medicine compositions prepared in Examples 1-3 and Comparative Examples 1-4 were diluted to a Chinese medicine solution with a concentration of 0.5 g / mL for later use;

[0056] Commercially available medicine Ganmao Qingre Granule, batch number: 20230721, Beijing Tongrentang;

[0057] Influenza A virus (H1N1) strain A / FM1 / 47;

[0058] A total of 110 ICR mice (SPF / VAF grade), half male and half female, with an average age of 3.6±0.4 weeks and a body weight of 18.6±1.0 g, were randomly selected to establish the influenza virus model, and the remaining 10 mice were set up as the control group.

[0059] (2) Construction of experimental model mice:

[0060] Fresh 9-day-old chicken embryo allantoic fluid (hemagglutination titer 1:1280) after influenza virus amplification was diluted 10 times to 6 concentrations. After the mice were anesthetized by ether inhalation, 0.05 mL of allantoic fluid was inoculated into the nasal cavity of each mouse. The mice were observed for 7 consecutive days. The modeling was successful if runny nose, sneezing, coughing, chills, coma, diarrhea, and increased wheezing occurred.

[0061] (3) Trial groups and dosing regimen:

[0062] 90 mice with successful influenza virus modeling were selected and randomly divided into a model group, a Ganmao Qingre Granule group, Example 1 group, Example 2 group, Example 3 group, Comparative Example 1 group, Comparative Example 2 group, Comparative Example 3 group, and Comparative Example 4 group, with 10 mice in each group.

[0063] The original drug dose for mice was 0.5 g / kg / d (corresponding to 1 mL / kg / d of Chinese medicine solution) according to the conversion relationship between human dose and mouse gavage dose. The Example 1-3 group and the Comparative Example 1-4 group were gavaged at 0.5 g / kg / d, and the model group and the blank control group were gavaged with the same volume of sterile distilled water for 5 consecutive days.

[0064] (4) Test method:

[0065] After 5 days of treatment, 10 groups of mice were anesthetized with 5 mL of 10% chloral hydrate intraperitoneally, intubated and assisted with breathing by a ventilator, and the following pulmonary function indicators were measured using a spirometer: 50% forced expiratory flow (FEF50), 75% forced expiratory flow (FEF75), and peak expiratory flow (PEF).

[0066] (5) Test results:

[0067] The above test results are shown in Table 1:

[0068] Table 1 Comparison of lung function indicators of mice in each group ( n=10)

[0069]

[0070]

[0071] The results showed that the FEF50, FEF75 and PEF of the model group were significantly lower than those of the control group; the Example 1-3 group and the Ganmao Qingre Granule group were significantly higher than the model group, and there was no significant difference with the blank control group; the effect of the comparative example 1 and 2 groups after omitting the monarch drug was significantly reduced compared with the embodiment, indicating that the present invention can effectively alleviate the lung damage caused by influenza and increase lung capacity. The comparative example 3 and 4 groups omitted one ministerial drug, and the effect of the other ministerial drug was better than that of the comparative example 1 and 2 groups after adding the amount, but worse than the embodiment group, indicating that the two ministerial drugs played a synergistic role in improving lung compliance.

[0072] Test Example 2

[0073] The main purpose is to test the therapeutic effect of the Chinese medicine composition of the present invention on lung inflammation in influenza virus model mice.

[0074] (1) Test materials and drugs: Same as in Test Example 1.

[0075] (2) Construction of experimental model mice: Same as in Experimental Example 1.

[0076] (3) Experimental grouping and dosing regimen: Same as Experimental Example 1.

[0077] (4) Test method:

[0078] On the 5th day after administration, blood was collected from the orbital venous plexus of mice in each group, and serum was collected by centrifugation. Then, ELISA kits were used to detect the levels of inflammatory factors IL-1β, TNF-α, and IFN-γ in the mouse serum, and the results were compared among the groups (n=10).

[0079] (5) Test results:

[0080] The above test results can be found in Figure 1 , Figure 2 and Figure 3 , Figure 1-3 The results showed that compared with the control group, viral infection could significantly increase the content of inflammatory factors IL-1β, TNF-α, and IFN-γ in the serum of model mice; compared with the model group, after treatment with Example 1, Example 2, and Example 3, the levels of IL-1β, TNF-α, and IFN-γ in serum could be significantly reduced, and the lung inflammation of mice could be improved; the comparative example groups 3 and 4 omitted one of the adjuvants, and the effect of the other adjuvant was better than that of the comparative example groups 1 and 2, but worse than that of the embodiment group, which also showed that the two adjuvants played a synergistic role and could effectively alleviate the lung inflammation caused by influenza.

[0081] Test Example 3

[0082] The main purpose is to verify the therapeutic effect of the Chinese medicine composition of the present invention on cough in mice.

[0083] (1) Experimental animal grouping and dosing regimen:

[0084] Another 90 ICR mice were randomly divided into a model group, a Ganmao Qingre Granule group, Example 1 group, Example 2 group, Example 3 group, Comparative Example 1 group, Comparative Example 2 group, Comparative Example 3 group, and Comparative Example 4 group, with 10 mice in each group.

[0085] The Example 1-3 group and the Comparative Example 1-4 group were intragastrically administered with 0.5 g / kg (0.5 g of the Chinese medicine solution containing the raw material drug per kg of mouse), and the model group was intragastrically administered with an equal volume of sterile distilled water, and the treatment was continued for 5 days.

[0086] (2) Test method:

[0087] The mice in the other groups were gavaged for 5 consecutive days. 3 minutes after the last administration, the mice in the other groups were placed in a 500 mL glass bell jar. A glass spray head was connected to the gas compressor, and ammonia water (25% to 28%) was sprayed evenly into the bell jar at a constant pressure of 400 mmHg (1 mmHg = 0.133 kPa) for 5 seconds. The cough latency and the number of coughs within 2 minutes of each group of mice were observed and recorded, and the results were compared among the groups.

[0088] (3) Test results:

[0089] The test results are shown in Table 2:

[0090] Table 2 Statistics of cough latency and cough times within 2 min in each group of mice ( n=10)

[0091]

[0092]

[0093] The statistical results of the cough latency and the number of coughs within 2 minutes in each group of mice showed that compared with the model group, the cough latency of the Ganmao Qingre Granule group and the Example 1-3 groups was significantly prolonged, and the number of coughs within 2 minutes was also significantly reduced, indicating that the Chinese medicine composition of the present invention has the effect of prolonging the cough latency and reducing the number of coughs; the comparative example 3 and 4 groups omitted one of the adjuvants, and the effect of the other adjuvant was better than that of the comparative example 1 and 2 groups after the dosage was increased, but worse than that of the example group, which also shows that the two adjuvants play a synergistic role and can effectively relieve the cough symptoms caused by influenza.

[0094] The above-mentioned embodiments are only preferred embodiments of the present invention and cannot be used to limit the scope of protection of the present invention. Any non-substantial changes and substitutions made by technicians in this field on the basis of the present invention shall fall within the scope of protection required by the present invention.

Claims

1. A Chinese medicine composition for treating influenza, characterized in that: The invention is composed of the following raw materials in parts by weight: 10-20 parts of white wormwood, 15-25 parts of walnut shells, 8-11 parts of kiwi fruit roots, 6-10 parts of rutabaga seeds, 5-7 parts of fried yam with bran, 3-5 parts of platycodon, 2-5 parts of atractylodes macrocephala and 1-2 parts of liquorice.

2. The Chinese medicine composition for treating influenza according to claim 1, characterized in that: The drug is composed of the following raw materials in parts by weight: 15 parts of white wormwood, 22 parts of walnut shells, 10 parts of kiwi fruit roots, 8 parts of rutin seeds, 6 parts of fried yam with bran, 4 parts of platycodon, 4 parts of atractylodes, and 2 parts of liquorice.

3. The method for preparing the Chinese medicine composition for treating influenza according to claim 1 or 2, characterized in that: Weigh each raw material drug according to the ratio, crush it, put the crushed raw material drug into an extraction tank, soak it in water, extract it at 45°C-65°C and the steam pressure value in the extraction tank is 0.045-0.048 Mpa, filter it, and concentrate it to a total concentration of each raw material drug of 1-2 g / mL to obtain the traditional Chinese medicine composition for treating influenza.

4. The method for preparing the Chinese medicine composition for treating influenza according to claim 3, characterized in that: The weight ratio of the total weight of each raw material drug to water is 1:10-20.

5. The method for preparing the Chinese medicine composition for treating influenza according to claim 3, characterized in that: The soaking time is 30-60 min, and the extraction time is 1-2 h.

6. The method for preparing the Chinese medicine composition for treating influenza according to claim 3, characterized in that: Pharmaceutically acceptable excipients are added to the traditional Chinese medicine composition for treating influenza to prepare a corresponding dosage form.

7. The method for preparing the Chinese medicine composition for treating influenza according to claim 6, characterized in that: The dosage form is an oral dosage form.

8. The method for preparing the Chinese medicine composition for treating influenza according to claim 7, characterized in that: The oral dosage form is powder, oral liquid, granule, capsule, tablet or pill.

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