A composite natural enzyme capsule for degreasing and its preparation method

Abstract

The present invention relates to a composite natural enzyme capsule for degreasing and a preparation method thereof, belonging to the technical field of degreasing composite enzymes. In the present invention, a fermentation product is obtained by fermenting humus soil, basalt powder, straw powder, sucrose and deionized water, and then the composite natural enzyme capsule is obtained by mixing and filling the fermentation product, composite amino acids, magnesium stearate and corn starch. The composite natural enzyme capsule is non-toxic, non-irritating, non-corrosive, almost odorless, can be widely used for the treatment and purification of kitchen waste oil stains such as sewers, and can promote the growth of fungi in the sewer environment.

Description

Technical Field

[0001] The present invention belongs to the technical field of degreasing composite enzymes, and relates to a composite natural enzyme capsule for degreasing and a preparation method thereof. Background Art

[0002] Edible oil refers to animal or vegetable oils used in the process of making food. Oil has two forms, liquid and solid, depending on the temperature. Due to reasons such as raw material sources, processing techniques, and quality, common edible oils are mostly vegetable oils, including soybean oil, rapeseed oil, etc., and animal oils include duck oil, lard, and other animal oils.

[0003] In the catering industry, a large amount of difficult-to-clean oil stains are mostly removed by substances such as gasoline, kerosene, benzene, and commercial degreasers. Gasoline, kerosene, benzene, and commercial degreasers all belong to organic solvents, surfactants, or hot alkali solutions. When used to remove oil stains, they often cause relatively serious environmental pollution, or have relatively strong corrosiveness, or the water body is difficult to treat after cleaning, resulting in high usage costs.

[0004] Kitchen sewer wastewater contains a large amount of vegetable and animal oils. The kitchen waste oil stains in the sewer are likely to cause sewer blockages and produce foul odors. In industries such as household kitchens, restaurants, hotels, and canteens, there are problems such as incomplete oil-water separation in the physical method of recovering oil. The biological bacteria degradation technology can degrade most of the kitchen waste oil stains in the sewer. The biological bacteria degradation technology is a relatively ideal treatment method, but the number of strains such as Bacillus subtilis and EM bacteria available for oil degradation in the kitchen sewer is relatively small, so the degradation ability is low.

[0005] In summary, it is particularly important to find a natural substance that can promote the rapid reproduction of Bacillus subtilis, EM bacteria, etc. in the kitchen sewer, and is non-toxic, odorless, non-irritating, non-corrosive, and has low required costs. Summary of the Invention

[0006] The purpose of the present invention is to provide a composite natural enzyme capsule for degreasing and a preparation method thereof. The prepared composite natural enzyme capsule can promote the degradation of kitchen waste oil stains (including vegetable oil and animal oil) and the elimination of odors.

[0007] The purpose of the present invention can be achieved by the following technical solutions:

[0008] A composite natural enzyme capsule for oil removal, wherein the wall material of the composite natural enzyme capsule is corn starch, and the core material is a mixture of fermentation products, compound amino acids, magnesium stearate and corn starch. The fermentation products are obtained by fermenting humus soil, basalt powder, straw, sucrose and deionized water. In the mixture, the lipase content is ≥0.1%, the protease content is ≥0.1%, the cellulase content is ≥0.1%, and the spore bacteria content is ≥30×10 8 CFU / mL.

[0009] As a preferred technical solution of the present invention, the lipase content in the composite natural enzyme capsule is 0.1-0.5%.

[0010] A preparation method of a composite natural enzyme capsule for oil removal, comprising the following preparation process:

[0011] S1. Mix humus soil, basalt powder, straw powder, sucrose and deionized water according to a volume ratio of (0.8-1.5):(2-3.5):(3-4.5):(0.3-0.5):(0.5-1), and ferment to obtain fermentation products;

[0012] S2. Mix the fermentation products, compound amino acids, magnesium stearate and corn starch according to a mass ratio of (0.40-0.50):(0.03-0.05):(0.15-0.25):(0.25-0.35) to prepare a mixture, and fill the mixture to obtain the composite natural enzyme capsule for oil removal.

[0013] As a preferred technical solution of the present invention, in step S1, the fermentation conditions are: ferment at 26-28°C and pH 7-8 for 72-144 h.

[0014] As a preferred technical solution of the present invention, in step S1, when the fermentation products are fermented, when the lipase content is ≥0.1% determined by the NaOH titration method and the spore bacteria content is ≥30×10 8 CFU / mL as the discharging index of the fermentation tank, the fermentation can be stopped.

[0015] As a preferred technical solution of the present invention, in step S1, the straw powder is prepared by pulverizing corn straw with a pulverizer and passing through a 60-mesh sieve.

[0016] As a preferred technical solution of the present invention, in step S2, the filling is carried out by a filling capsule plate filling machine, and the filling conditions are: temperature 19-24°C, relative humidity 35-60%.

[0017] As a preferred technical solution of the present invention, the compound amino acid is an equimass ratio mixture of twenty-three amino acids, namely tryptophan, methionine, threonine, valine, lysine, histidine, leucine, isoleucine, alanine, phenylalanine, cystine, cysteine, arginine, glycine, serine, tyrosine, 3,5-diiodotyrosine, glutamic acid, aspartic acid, proline, hydroxyproline, citrulline, and ornithine.

[0018] The humus soil used in the present invention is selected from the humus soil of the pine needle forest cultivated in Changbai Mountain, Northeast China. The black soil in Northeast China is formed after the surface vegetation dies and accumulates and evolves into humus after a long time under the conditions of a temperate semi-humid climate zone and cold climate. Because it takes 400 years to form a 1-cm-thick black soil layer, the content of humus and organic matter in the soil layer is extremely rich, and the soil is fertile. In addition to the above advantages, the humus soil of the pine needle forest cultivated in Northeast China for 3-4 years has a large amount of aerobic bacteria such as Bacillus subtilis, EM bacteria (including photosynthetic bacteria group, yeast bacteria group, actinomycetes group, etc.), Pseudomonas, Aeromonas, Agrobacterium, etc. multiplying due to the rich content of pine needles and relatively large soil pores.

[0019] In the present invention, humus soil, basalt powder, straw powder, sucrose, and deionized water are used as raw materials for fermentation. During the fermentation process, the straw powder uses microorganisms to decompose the organic substances in the straw into organic fertilizers. At the same time, the straw is rich in cellulose, hemicellulose, and lignin and can provide trace elements. Therefore, it can promote the production of lipase.

[0020] The humus soil can provide a large number of microorganisms and bacterial sources because it can provide a large amount of trace elements and nitrogen sources and can also serve as a substrate for bacterial growth. Sucrose provides a carbon source. The straw powder is rich in cellulose, hemicellulose, and lignin, making the sources of sugar (carbon) and water more diverse. The basalt powder not only provides trace elements but also makes the whole system more porous and breathable, ensuring sufficient oxygen. Under suitable fermentation conditions, it can form natural lipase and other organic substances with rich nutrients and a large number of microbial flora such as Bacillus and EM bacteria genus that have the function of decomposing oils and fats. At the same time, because substances such as organic acids produced during the straw fermentation process will further promote the reproduction of beneficial bacteria; sucrose contains rich amino acids and proteins and can be decomposed into monosaccharides, thus providing a carbon source for microorganisms; humus soil and basalt powder are used to provide trace elements, straw provides fermentation raw materials, and sucrose promotes catalytic fermentation.

[0021] Among the raw materials for preparing the compound natural enzyme of the present invention, the fermentation product, compound amino acid, and corn starch can provide energy and nutrients for the reproduction of fungi, and corn starch and magnesium stearate are mainly used as auxiliary materials for capsule forming.

[0022] The beneficial effects of the present invention:

[0023] The present invention provides a composite natural enzyme, including lipase, protease and cellulase. Lipase can hydrolyze triglycerides into glycerol and fatty acids, a small amount of carbon dioxide, water, sugars, etc. The products of protease decomposing proteins are mainly amino acids and polypeptides. Cellulase is a composite enzyme, mainly composed of exo-β-glucanase, endo-β-glucanase and β-glucosidase, etc. These enzymes act synergistically to degrade cellulose into glucose. Humus soil, twenty-three trace elements, starch, etc. are also added to this natural composite enzyme, which can meet the nutrients such as carbon source, nitrogen source, inorganic salts, growth factors, etc. required for the rapid reproduction of microorganisms. Regular addition and use can promote the growth of microbial colonies in the kitchen waste environment of the sewer, thereby enhancing the oil removal effect, and thus can promote the degradation of kitchen waste oil (including vegetable oil and animal oil) and the elimination of odors in the sewer. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] For the convenience of those skilled in the art to understand, the present invention will be further described below with reference to the accompanying drawings.

[0025] Figure 1 It is a HE staining picture of the heart tissue of rats after a single intragastric administration of the maximum amount of the composite natural enzyme for 14 days;

[0026] Figure 2 It is a HE staining picture of the liver tissue of rats after a single intragastric administration of the maximum amount of the composite natural enzyme for 14 days;

[0027] Figure 3 It is a HE staining picture of the kidney tissue of rats after a single intragastric administration of the maximum amount of the composite natural enzyme for 14 days;

[0028] Figure 4 It is a HE staining picture of the lung tissue of rats after a single intragastric administration of the maximum amount of the composite natural enzyme for 14 days;

[0029] Figure 5 It is a HE staining picture of the spleen tissue of rats after a single intragastric administration of the maximum amount of the composite natural enzyme for 14 days;

[0030] Figure 6 It is a HE staining picture of the small intestine tissue of rats after a single intragastric administration of the maximum amount of the composite natural enzyme for 14 days;

[0031] Figure 7 It is a HE staining picture of the brain tissue of rats after a single intragastric administration of the maximum amount of the composite natural enzyme for 14 days;

[0032] Figure 8 The skin irritation effect of the composite natural enzyme on rats;

[0033] Figure 9 It is the difference in absorbance of different composite natural enzyme capsules promoting bacterial growth;

[0034] Figure 10 Oil removal effect diagrams of different composite natural enzyme capsules;

[0035] Figure 11 Oil removal rates of different composite natural enzyme capsules;

[0036] Figure 12 Shows the changes in the condensate in the dishwashing water filter pool after the composite natural enzyme capsules prepared in Example 1 were put in. Among them, (A) is before putting in; (B) is 24 hours after putting in; (C) is 72 hours after putting in. Detailed implementation manners

[0037] To further elaborate on the technical means and effects adopted by the present invention to achieve the predetermined invention purpose, the following combines examples to detail the specific implementation manners, structures, features and their effects according to the present invention as follows.

[0038] In the comparative examples and examples of the present invention:

[0039] Unless otherwise specified, the room temperature is 23±2°C.

[0040] The capsules are edible corn starch hollow capsule shells purchased;

[0041] The composite amino acids are prepared by mixing tryptophan, methionine, threonine, valine, lysine, histidine, leucine, isoleucine, alanine, phenylalanine, cystine, cysteine, arginine, glycine, serine, tyrosine, 3,5-diiodotyrosine, glutamic acid, aspartic acid, proline, hydroxyproline, citrulline, ornithine, etc. in equal mass ratios.

[0042] Amino acids: All are purchased from Henan Wanbang Industrial Co., Ltd.;

[0043] Porous basalt powder: Purchased from the Northern Volcanic Rock Wholesale Center, and after purchase, it is pulverized by a pulverizer and filtered through an 80-mesh sieve;

[0044] Humus soil: Selected from the humus soil of the pine needle forest cultivated in Changbai Mountain, Northeast China, purchased from Shenzhibei Organic Soil , with organic black matter > 30%, pH value 4.8 - 8.5, and the implementation standard is Q / SZB001-2018);

[0045] Magnesium stearate: Purchased from Shanghai Yuanye Bio-Technology Co., Ltd., product number: S24259;

[0046] Corn starch: Purchased from Shanghai Yuanye Bio-Technology Co., Ltd., product number: S11149;

[0047] Sucrose: Purchased from Shanghai Macklin Biochemical Co., Ltd., product number: S818044.

[0048] Example 1

[0049] S1. Mix humus soil, porous basalt powder, corn straw powder, sucrose, and deionized water in a volume ratio of 1.2:2.8:4.1:0.4:0.7, and then ferment for 96 h at a temperature of 27 °C and a pH of 7.5. The lipase content is determined to be 0.37% by the NaOH titration method, and the spore bacteria content is measured to be 35×10 8 CFU / mL by the hemocytometer method. Stop fermentation to obtain the fermentation product;

[0050] S2. Mix the above fermentation product with compound amino acids, magnesium stearate, and corn starch in a mass ratio of 0.46:0.04:0.19:0.31, and fill it with a filling capsule plate filling machine to obtain a compound natural enzyme capsule containing compound natural enzymes. Among them, the capsule filling conditions include: temperature 22 °C, relative humidity 54%.

[0051] Example 2

[0052] S1. Mix humus soil, porous basalt powder, corn straw powder, sucrose, and deionized water in a volume ratio of 0.8:2:3:0.3:0.5, and then ferment for 96 h at a temperature of 27 °C and a pH of 7.5. The lipase content is determined to be 0.33% by the NaOH titration method, and the spore bacteria content is measured to be 33×10 8 CFU / mL when stopping fermentation to obtain the fermentation product;

[0053] S2. Mix the above fermentation product with compound amino acids, magnesium stearate, and corn starch in a mass ratio of 0.40:0.03:0.15:0.25, and fill it with a filling capsule plate filling machine to obtain a compound natural enzyme capsule containing compound natural enzymes. Among them, the capsule filling conditions include: temperature 22 °C, relative humidity 54%.

[0054] Example 3

[0055] S1. Mix humus soil, porous basalt powder, corn straw powder, sucrose, and deionized water in a volume ratio of 1.5:3.5:4.5:0.5:1, and then ferment for 96 h at a temperature of 27 °C and a pH of 7.5. The lipase content is determined to be 0.39% by the NaOH titration method, and the spore bacteria content is measured to be 37×10 8 CFU / mL. Stop fermentation to obtain the fermentation product;

[0056] S2. Mix the above fermentation product with compound amino acids, magnesium stearate, and corn starch in a mass ratio of 0.50:0.05:0.25:0.35, and fill it with a filling capsule plate filling machine to obtain a compound natural enzyme capsule containing compound natural enzymes. Among them, the capsule filling conditions include: temperature 22 °C, relative humidity 54%.

[0057] Example 4

[0058] S1. Mix humus soil, porous basalt powder, corn straw powder, sucrose and deionized water in a volume ratio of 1.0:2.2:3.5:0.3:0.6, and then ferment at a temperature of 27 °C and a pH of 7.5 for 96 h. The lipase content is determined by the NaOH titration method to be 0.36%, and the spore bacteria content is measured by the hemocytometer method to be 37×10 8 CFU / mL, stop fermentation to obtain a fermentation product;

[0059] S2. Mix the above fermentation product with compound amino acids, magnesium stearate and corn starch in a mass ratio of 0.42:0.03:0.17:0.28, and fill it with a filling capsule plate filling machine to obtain a compound natural enzyme capsule containing compound natural enzymes. Among them, the capsule filling conditions include: temperature 22 °C, relative humidity 54%.

[0060] Example 5

[0061] S1. Mix humus soil, porous basalt powder, corn straw powder, sucrose and deionized water in a volume ratio of 1.4:3.0:4.3:0.5:0.9, and then ferment at a temperature of 27 °C and a pH of 7.5 for 96 h. The lipase content is determined by the NaOH titration method to be 0.38%, and the spore bacteria content is measured by the hemocytometer method to be 37×10 8 CFU / mL, stop fermentation to obtain a fermentation product;

[0062] S2. Mix the above fermentation product with compound amino acids, magnesium stearate and corn starch in a mass ratio of 0.48:0.05:0.22:0.34, and fill it with a filling capsule plate filling machine to obtain a compound natural enzyme capsule containing compound natural enzymes. Among them, the capsule filling conditions include: temperature 22 °C, relative humidity 54%.

[0063] Comparative Example 1

[0064] This comparative example provides an ordinary soil capsule.

[0065] The difference between Comparative Example 1 and Example 1 is that the humus soil is replaced with ordinary soil (organic matter content < 7%, pH value 4.5 - 8.5, implementation standard GB15618 - 2008); the remaining operations are the same. For the prepared ordinary soil capsule, the lipase content determined by the NaOH titration method is 0.16%.

[0066] Comparative Example 2

[0067] This comparative example provides a capsule without amino acids.

[0068] The difference between Comparative Example 2 and Example 1 is that the compound amino acid was replaced with an equal amount of corn starch, and the rest of the operations were the same. The obtained capsule without amino acid was determined by the NaOH titration method to have a lipase content of 0.24%.

[0069] Performance test:

[0070] 1. Acute toxicity experiment, taking the compound natural enzyme capsule prepared in Example 1 as an example:

[0071] Twenty SD rats were selected. After 7 days of adaptive feeding, they were randomly divided into a normal group and a medicated group, with 10 rats in each group, half male and half female. The maximum dilution concentration that can be gavaged with this compound natural enzyme capsule is 1.5 g / mL, the gavage volume is 2 mL / (100 g·bw), and the medicated group was given 30 g / kg at one time. Before gavage, they were fasted but not water-deprived for 14 h. The normal group was gavaged with an equal volume of water. After gavage, they continued to fast for 4 h and were continuously observed for 14 days. The weights of the two groups of rats were weighed and recorded every day, including the food intake and water intake. After the 14-day observation period of the rats, blood was taken by removing the eyeballs, and the white blood cells (WBC), red blood cells (RBC), hemoglobin (HGB), platelets (PLT), and lymphocytes (LYM) of each group of rats were detected using a blood routine analyzer. The measurement data of the experimental results are expressed as the mean ± standard deviation indicating that the comparison of data between groups was performed using one-way ANOVA and t-test, with P < 0.05 indicating a statistically significant difference. During the 14-day observation period, no rats died in each group, and there were no obvious abnormalities in the back hair, skin color, nose, eyes, and oral secretions of the rats. The experimental results showed that after a one-time maximum dose of the natural compound enzyme was gavaged to rats at one time for 14 days, the body weights, diets, water intakes, blood routines, and HE staining of the main organs of male and female rats were not affected. It shows that the compound natural enzyme has no obvious acute toxicity reaction and has a relatively large safe application range.

[0072] Table 1 Effects of the acute toxicity experiment of the compound natural enzyme capsule on the body weights of rats ( g)

[0073] Time Control Group (Female) Medication Group (Female) Control Group (Male) Medication Group (Male) 0d 175.12±2.46 176.23±1.98 247.23±3.41 246.78±2.43 2d 179.43±3.42 180.11±4.02 252.41±3.49 250.56±3.23 4d 184.11±2.24 183.22±3.01 259.36±2.43 260.12±3.01 6d 189.91±3.11 189.03±2.94 269.71±3.48 271.02±3.01 8d 197.67±4.01 198.64±3.89 278.45±4.01 280.11±3.67 10d 206.23±5.11 207.25±4.31 287.89±4.40 289.02±3.48 12d 214.32±4.88 215.44±5.21 298.10±3.34 297.47±4.01 14d 220.92±3.46 222.43±4.27 307.23±4.04 306.87±3.58

[0074] Table 2 Effects of the acute toxicity experiment of the compound natural enzyme capsule on the food intakes of rats ( g)

[0075] Time Control Group (Female) Medication Group (Female) Control Group (Male) Medication Group (Male) 0d 5.88±1.41 5.43±1.54 7.23±1.46 7.76±2.07 2d 8.44±1.02 7.22±1.31 10.56±2.17 11.01±2.47 4d 7.84±2.01 8.97±2.15 12.47±3.69 12.51±2.70 6d 10.03±1.56 9.01±2.76 13.57±3.05 14.00±3.21 8d 8.70±1.67 8.97±1.97 12.46±2.56 13.11±2.49 10d 7.02±1.98 7.22±2.26 13.60±3.41 14.05±3.07 12d 8.67±2.21 7.05±2.03 14.25±3.36 14.03±3.25 14d 8.89±2.25 7.92±1.87 14.11±2.97 14.72±3.02

[0076] Table 3 Effects of the acute toxicity experiment of the compound natural enzyme capsule on the water intakes of rats ( g)

[0077] Time Control Group (Female) Medication Group (Female) Control Group (Male) Medication Group (Male) 0d 12.11±3.35 12.71±3.57 20.57±4.56 19.03±3.99 2d 20.33±2.86 20.35±3.70 27.90±5.03 26.37±4.06 4d 22.43±4.75 23.11±3.80 32.11±4.45 31.87±4.35 6d 24.24±4.73 25.11±4.02 34.66±4.23 31.63±3.89 8d 24.77±3.88 24.99±4.02 34.56±5.07 32.56±4.78 10d 25.89±4.22 26.59±3.89 33.13±4.57 32.66±5.21 12d 26.01±3.97 27.50±4.52 33.44±5.01 34.09±4.78 14d 26.79±4.11 26.78±4.59 32.05±4.27 35.24±5.02

[0078] Table 4 Effects of Acute Toxicity Experiment of Compound Natural Enzyme Capsules on Blood Routine Indexes of Rats( g)

[0079]

[0080] Meanwhile, through the HE observation diagrams of the main organs of rats after intragastric administration of the maximum dose of compound natural enzyme once for 14 days, it can be seen that according to Figure 1 it can be obtained that the muscle fibers of rats are arranged neatly in bundles, the morphology and size of cardiomyocytes and cell nuclei are normal, and there are no tissue vacuolization, tissue edema and inflammatory infiltration; according to Figure 2 it can be seen that the liver tissue structure is complete, the hepatocyte morphology is normal, there is no inflammatory cell infiltration, and the hepatocytes are arranged radially around the central vein; from Figure 3 it can be seen that the glomeruli and renal tubules have normal structures, there is no hyperplasia of mesangial cells and matrix, and there is no inflammatory cell infiltration and fibrosis in the renal interstitium; from Figure 4 it can be seen that the inner wall of the rat lung airway is smooth without obvious thickening, there is no inflammatory cell infiltration around, the overall structure of the alveolar cavity is complete, the inner wall is smooth, there is no fusion or defect, no obvious bleeding is seen, and there is no inflammatory cell infiltration; according to Figure 5 it can be seen that the boundary between the red pulp and white pulp of the spleen is obvious, there is no obvious congestion in the splenic blood sinus, and the splenic corpuscle structure is clear; from Figure 6 it can be seen that the intestinal wall of the rat small intestine is complete and the structure is clear, the epithelial cells are arranged orderly, the villi are relatively smooth, and the glands are normal without inflammatory cell infiltration; from Figure 7 it can be seen that the cerebral cortex of the rat is clearly structured, the nerve cell structure is complete, and there is no inflammatory cell infiltration. The nerve fibers in the white matter are evenly stained, light pink, arranged neatly and with clear texture.

[0081] 2. Skin irritation experiment, taking the compound natural enzyme capsule prepared in Example 1 as an example:

[0082] Six healthy SPF-grade rats, half male and half female, were randomly divided into two groups: blank control group and single-dose administration group. Before the experiment, a hair clipper was used to shave the fur on the unilateral mid-abdomen of 6 rats (half male and half female) with an area of 3 cm × 3 cm to form a normal skin area. The other 6 rats (half male and half female) were scratched with a scalpel on the epidermis after shaving. The blank control group was smeared with normal saline, and the unilateral skin was smeared with a suspension of compound natural enzyme at 2.0 g / mL. After smearing evenly, it was covered with a gauze and fixed with medical tape, and removed after 4 h( Figure 8 A / Figure 8 C), and whether there are phenomena such as erythema and edema at the smeared site was observed at 24 h, 48 h, and 72 h( Figure 8 ) respectively, and the erythema and edema were scored according to the Draize scoring system.

[0083] After different periods of time, no erythema or edema occurred at the applied sites of the rats. Therefore, this natural composite enzyme capsule has no irritating effect on the skin of rats.

[0084] Therefore, based on the above tests, it is proved that the composite natural enzyme capsule prepared by the present invention is non-toxic.

[0085] 3. Determination of the effect of promoting the growth of bacterial strains

[0086] Equal weights of the composite natural enzyme capsules prepared in Examples 1-5 of the present invention, the ordinary soil capsules prepared in Comparative Example 1, and the capsules without amino acids prepared in Comparative Example 2 were respectively put into 45 mL of LB medium (in the medium Trypsin Peptone 10 g / L, Yeast Extract 5 g / L, sodium chloride 10 g / L), and after static culture in an incubator at 37 °C for 60 h, the absorbance of the supernatant of the medium at different culture times was measured. The results are recorded in Table 5 and Figure 9 .

[0087] Table 5 Statistics of the growth promotion of bacterial strains

[0088]

[0089] Note: The absorbance of 0.6-0.8 is the logarithmic growth phase of bacteria; from Table 5 and Figure 9 it can be seen that as the culture time increases, the OD value of the medium gradually increases, and the OD values after 60 h in the examples are all higher than those in the comparative examples, and the increased values are also much higher than those in the comparative examples, indicating that the bacterial concentration and the number of cells in the medium treated by the examples are higher, so more light is absorbed, indicating that the composite natural enzyme capsule prepared by the present invention can increase the number of bacteria in the original environment and promote the growth of bacteria when applied. And the composite natural enzyme capsule prepared in Example 1 of the present invention has a significant increase in promoting the growth of bacteria at 60 h compared with the other two groups.

[0090] 4. Determination of the oil removal effect

[0091] One capsule each of the composite natural enzyme capsule prepared in Example 1 of the present invention, the ordinary soil capsule prepared in Comparative Example 1, and the capsule without amino acids prepared in Comparative Example 2 with equal weights (the filling amount of each capsule is 1 g) were respectively put into 45 mL of deionized water, 5 mL of Jinlongyu soybean oil (A1) was added, and after maintaining air at room temperature for 72 h, the volume of the remaining Jinlongyu soybean oil (A2) in each group was measured, and the oil removal rate was calculated:

[0092] Oil removal rate (%) = (A1 - A2) / A1;

[0093] According to Figure 10It can be seen that the oil removal effect of the composite natural enzyme capsules prepared in Example 1 is the best, and the remaining oil volume is the lowest.

[0094] According to Figure 11 It can be seen that the degreasing rate of the composite natural enzyme capsule group (Example 1) is 88.3% higher than that of the ordinary soil capsule group (Comparative Example 1), and the degreasing rate of the composite natural enzyme capsule group (Example 1) is 23.7% higher than that of the capsule group without amino acids (Comparative Example 2).

[0095] Figure 11 In ** <0.01, there is a significant difference between the two groups; compared with the capsule group without amino acids (Comparative Example 2), P ## <0.01, there is a significant difference between the two groups, indicating that the oil removal rate of the composite natural enzyme capsule group is significantly higher than that of the ordinary capsule group and the capsule group without amino acids.

[0096] 5. Determination of the effect on the condensate in the dishwashing water filter

[0097] According to Figure 12 It can be seen that under normal temperature conditions, after the composite natural enzyme capsules prepared in Example 1 of the present invention are put into the dishwashing water filter at a cumulative mass ratio of 1:2000 for 3 consecutive days, the condensate becomes thinner and the fluidity increases significantly.

[0098] The above is only a preferred embodiment of the present invention, and it does not impose any form of limitation on the present invention. Although the present invention has been disclosed above with a preferred embodiment, it is not intended to limit the present invention. Any person skilled in the art can make some changes or modifications to equivalent embodiments by using the disclosed technical content within the scope of the technical solution of the present invention. However, as long as it does not depart from the content of the technical solution of the present invention, any brief modification, equivalent change and modification made to the above embodiments based on the technical essence of the present invention still fall within the scope of the technical solution of the present invention.

Claims

1. A composite natural enzyme capsule for degreasing, characterized in that, The wall material of the composite natural enzyme capsule is corn starch, and the core material is a mixture of fermentation products, compound amino acids, magnesium stearate and corn starch. The fermentation products are obtained by fermenting humus soil, basalt powder, straw, sucrose and deionized water. In the mixture, the lipase content is ≥ 0.1%, the protease content is ≥ 0.1%, the cellulase content is ≥ 0.1%, and the spore bacteria content is ≥ 30×10 8 CFU / mL; The preparation method of the composite natural enzyme capsule for degreasing includes the following preparation processes: S1. Mix humus soil, basalt powder, straw powder, sucrose and deionized water in a volume ratio of (0.8 - 1.5):(2 - 3.5):(3 - 4.5):(0.3 - 0.5):(0.5 - 1), and ferment to obtain a fermentation product. The conditions set during fermentation are: ferment at 26 - 28 °C and pH 7 - 8 for 72 - 144 h; S2. Mix the fermentation product, compound amino acids, magnesium stearate and corn starch in a mass ratio of (0.40 - 0.50):(0.03 - 0.05):(0.15 - 0.25):(0.25 - 0.35) to prepare a mixture, and fill the mixture into capsules to obtain the composite natural enzyme capsule for degreasing. The compound amino acids are an equimolar ratio mixture of twenty-three amino acids, namely tryptophan, methionine, threonine, valine, lysine, histidine, leucine, isoleucine, alanine, phenylalanine, cystine, cysteine, arginine, glycine, serine, tyrosine, 3,5-diiodotyrosine, glutamic acid, aspartic acid, proline, hydroxyproline, citrulline, ornithine.

2. The composite natural enzyme capsule for degreasing according to claim 1, wherein: The lipase content in the composite natural enzyme capsule is 0.1 - 0.5%.

3. The preparation method of the composite natural enzyme capsule for degreasing according to claim 1, characterized in that: In step S1, when the fermentation product is fermented, the lipase content is determined by the NaOH titration method to be ≥0.1%, and the spore bacteria content is measured by the hemocytometer method to be ≥30×10 8 CFU / mL of the discharging tank index, then the fermentation can be stopped.

4. The preparation method of the composite natural enzyme capsule for degreasing according to claim 1, characterized in that: In step S1, the straw powder is prepared by pulverizing corn straw with a pulverizer and passing through a 60-mesh sieve.

5. The preparation method of the composite natural enzyme capsule for degreasing according to claim 1, characterized in that: In step S2, during filling, it is filled with a filling plate filler, and the filling conditions are: temperature 19 - 24 °C, relative humidity 35 - 60%.

Images