A method for processing fluorescence immunoassay chromatography test data

Abstract

The present invention discloses a method for processing fluorescence immunoassay chromatography test data, which relates to the technical field of chromatography test data processing. First, a basic chromatography test is carried out to obtain each abnormal solution and each abnormal body in each abnormal solution. Then, a specific chromatography test is carried out. An initial specific chromatography test is set. According to the specific test data of the standard concentration of each abnormal solution, it is further determined whether it is necessary to dilute the specific chromatography test. When it is necessary to dilute the specific chromatography test, the specific test data of each diluted solution of each abnormal solution are analyzed to obtain the types and concentrations of abnormal bodies in each abnormal solution. Finally, the basic data of each solution and the types and concentrations of abnormal bodies in each abnormal solution are analyzed to determine whether it is necessary to give an early warning. The present invention can effectively process fluorescence immunoassay chromatography test data and improve the accuracy and reliability of test results.

Description

Technical Field

[0001] The present invention relates to the technical field of chromatographic test data processing, and particularly relates to a method for processing fluorescence immunoassay test data. Background Art

[0002] Fluorescence immunoassay technology is an efficient and rapid method for detecting specific pathogens in biological samples, and has been widely used in fields such as medicine and agriculture. With the continuous development of fluorescence immunoassay technology, the amount of data generated by tests is increasing, and how to effectively process and analyze this data has become increasingly important.

[0003] The prior art, such as the invention patent application with the publication number CN107389625B, discloses a method for processing fluorescence immunoassay test data, including providing an immunochromatographic test strip, an excitation light source, and a light receiving and reading device. Through the control device, the light spot is controlled to move along the length direction of the chromatographic test strip, and the signal intensities corresponding to the effective light spots are processed to obtain the detection result of the immunochromatographic test strip. The method of the present invention excludes factors such as batch-to-batch sample loss, batch-to-batch sample addition differences, and some interference factors during the antibody labeling process introduced on the quality control line, making the final data result have reasonable physical significance and higher sensitivity.

[0004] In view of the above solution, the present invention discovers that the above technology has at least the following technical problems: on the one hand, the above solution is only a method for detecting an abnormal body through an immunochromatographic test strip, and does not pre-detect the solution to be tested. If there are multiple abnormal bodies to be detected in a solution to be tested, this method needs to conduct experiments on the solution to be tested one by one, increasing the detection and analysis costs. On the other hand, the above solution only detects a single solution and does not consider the influence of multiple abnormal bodies on the chromatographic test strip, which is prone to false positives, affects the detection accuracy, is prone to collecting incorrect data, and reduces the sensitivity. Summary of the Invention

[0005] Aiming at the above existing technical deficiencies, the purpose of the present invention is to provide a method for processing fluorescence immunoassay test data.

[0006] To solve the above technical problems, the present invention adopts the following technical solutions: The present invention provides a method for processing fluorescence immunoassay test data, including the following steps: Step 1. Basic chromatographic test: Perform a basic chromatographic test on each analyte to obtain the fluorescence images of each solution, and then collect the basic chromatographic information of each solution from the fluorescence images of each solution, analyze the basic chromatographic information of each solution to obtain each abnormal solution, and identify each abnormal body in each abnormal solution from each abnormal solution.

[0007] Step 2. Specific chromatography test: Based on the abnormal substances in each abnormal solution, set up the initial specific chromatography test, collect the specific test data of each standard concentration of each abnormal solution, and then determine whether it is necessary to dilute the specific chromatography test. When performing the diluted specific chromatography test, collect the specific test data of each diluted solution of each abnormal solution. According to the solution specific model, analyze the specific test data of each diluted solution of each abnormal solution to obtain the types and concentrations of the abnormal substances in each abnormal solution.

[0008] Step 3. Chromatography data analysis: Obtain the basic solution data from the database, and according to the solution abnormal model, analyze the basic solution data, the types and concentrations of the abnormal substances in each abnormal solution to determine whether a warning is required.

[0009] Preferably, the analysis of the specific test data of each diluted solution of each abnormal solution is as follows: The specific test data of each diluted solution of each abnormal solution includes the abnormal substances in each diluted solution of each abnormal solution and the spot sizes corresponding to each abnormal substance. Count the abnormal substances in each diluted solution of each abnormal solution to obtain the occurrence times of each abnormal substance in each abnormal solution. Substitute the sizes of the specific spots of each diluted solution of each solution into the calculation formula to obtain the concentrations of the abnormal substances in each diluted solution of each abnormal solution. Obtain the standard concentration corresponding to each abnormal substance from the database. If the concentration of an abnormal substance in a certain diluted solution of a certain solution is greater than the standard concentration, it indicates that the concentration of the abnormal substance in the diluted solution of the solution is a high concentration, and count the occurrence times of the high concentration of each abnormal substance in each abnormal solution.

[0010] Input the occurrence times and high-concentration occurrence times of each abnormal substance in each abnormal solution into the solution specific model to obtain the output results of each abnormal solution. If the output result of each abnormal solution is 1, it indicates that the diluted specific chromatography test of the abnormal substance in the abnormal solution is accurate. If the output result is 0, it indicates that the diluted specific chromatography test of the abnormal substance in the abnormal solution is inaccurate, and replace the diluted solution and continue the diluted specific chromatography test.

[0011] Preferably, the analysis of the basic solution data, the types and concentrations of the abnormal substances in each abnormal solution is as follows: The basic solution data includes the standard concentration and the maximum concentration corresponding to each abnormal substance. Input the standard concentration corresponding to each abnormal substance into the solution abnormal model to obtain the output results of each solution. If the output result of a certain solution is 1 and the concentrations of all abnormal substances in the solution are less than the maximum concentrations corresponding to the respective abnormal substances, it indicates that the solution meets the requirements. If the output result of a certain solution is 0, or the concentration of an abnormal substance in the solution is greater than or equal to the maximum concentration corresponding to the respective abnormal substance, it indicates that the solution is abnormal and a warning is issued.

[0012] The beneficial effects of the present invention are as follows: 1. The present invention first conducts a basic chromatography test, performs a basic chromatography test on each analyte to obtain the fluorescence images of each solution, and then collects the basic chromatography information of each solution from the fluorescence images of each solution. Next, a specific chromatography test is carried out. According to each abnormal body in each abnormal solution, an initial specific chromatography test is set, and each specific test data of the standard concentration of each abnormal solution is collected. Then, it is judged whether it is necessary to dilute the specific chromatography test. When it is necessary to dilute the specific chromatography test, each specific test version data of each diluted solution of each abnormal solution is collected. According to the solution specific model, the specific test version data of each diluted solution of each abnormal solution is analyzed to obtain the types and concentrations of abnormal bodies in each abnormal solution. Then, the basic data of each solution and the types and concentrations of abnormal bodies in each abnormal solution are analyzed to judge whether it is necessary to give an alarm. The present invention pre-detects each solution through a basic chromatography test, and analyzes according to the basic chromatography information of each solution to obtain each abnormal solution and each abnormal body in each abnormal solution, meeting the detection requirements for multiple abnormal body solutions and reducing the costs of subsequent detection and analysis.

[0013] 2. The present invention compares the analysis result of the initial specific chromatography test data with the analysis result of the basic chromatography test data to judge whether the initial specific chromatography test data is accurate. If it is accurate, the initial specific chromatography test data is recorded as the specific chromatography test data. If it is not accurate, through the diluted specific chromatography test, the analysis result of the diluted specific chromatography test is recorded as the specific chromatography test data. The present invention reduces the probability of false positives and increases the analysis accuracy through the detection data analysis of multiple diluted solutions. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required for the description of the embodiments or the prior art. Obviously, the following drawings are only some embodiments of the present invention. For those of ordinary skill in the art, other drawings can be obtained based on these drawings without creative efforts.

[0015] Figure 1 It is a schematic flow chart of the implementation steps of the method of the present invention. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0016] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the drawings in the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, rather than all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts belong to the scope of protection of the present invention.

[0017] According to Figure 1As shown in the figure, the present invention provides a method for processing fluorescence immunoassay test data, including the following steps: Step 1, basic chromatography test: Perform basic chromatography tests on each analyte to obtain fluorescence images of each solution, and then collect basic chromatography information of each solution from the fluorescence images of each solution, analyze the basic chromatography information of each solution to obtain each abnormal solution, and identify each abnormal body of each abnormal solution from each abnormal solution.

[0018] In a specific embodiment, the collection of the basic chromatography information of each solution is as follows: The basic chromatography information of each solution includes the distance and fluorescence brightness of each light spot in each solution.

[0019] Obtain the types of each analyte from the database, select the fixing plate for the antigen to be detected according to the types of each analyte, prepare each analyte into a solution, then wash a part of each test solution into the fixing plate, and after chromatography, obtain the fluorescence image of each basic chromatography solution through a fluorescence observation device, and obtain the basic chromatography information of each solution from the fluorescence image of each basic chromatography solution.

[0020] It should be noted that the analyte refers to the item to be detected, such as blood, food or medicine. Different analytes require different antigens to be detected. At the same time, if the antigen to be detected has been obtained, there is no need to perform a basic chromatography test, and a specific chromatography test can be directly performed.

[0021] Through fluorescence recognition technology, the fluorescence image of each basic chromatography solution obtains the distances and fluorescence brightnesses of each light spot to the test line, selects the shortest distance from each light spot to the test line, and records it as the distance of each light spot, so as to obtain the distances and fluorescence brightnesses of each light spot in each solution.

[0022] It should be noted that fluorescence recognition technology identifies each fluorescence light spot through the brightness of each pixel point on the fluorescence image. The shortest distance is the antigen migration distance, and different antigens have different diffusion distances on the fixing agent.

[0023] In a specific embodiment, the analysis of the basic chromatography information of each solution is as follows: Obtain the fluorescence brightness and distance of each light spot of the standard solution from the database, and substitute the fluorescence brightness and distance of each light spot of the standard solution into the calculation formulas and , to obtain the brightness deviation value and distance deviation value between the x light spots of the standard solution, where x is the light spot number, , , and are the fluorescence brightness and distance of light spot x respectively, and They are the fluorescence brightness and distance of each light spot of the standard solution respectively. If the brightness deviation value of a pair of light spots of the standard solution is less than the preset light spot deviation value and the distance deviation value is less than the preset distance deviation value, it indicates that this pair of light spots of the standard solution is the same type of normal light spot. In this way, various types of normal light spots are obtained. The fluorescence brightness and distance of each light spot of the standard solution are statistically analyzed to obtain the fluorescence brightness and distance of each type of normal light spot.

[0024] It should be noted that the preset light spot deviation value and the preset distance deviation value are set by the staff respectively. For example, the preset light spot deviation value is 0.13 and the preset distance deviation value is 0.11.

[0025] The maximum fluorescence brightness and the minimum fluorescence brightness of each type of normal light spot are respectively recorded as the upper limit and the lower limit of the fluorescence brightness interval. In this way, the fluorescence brightness interval of each type of normal light spot is obtained. The shortest distance and the maximum distance of each type of normal light spot are respectively recorded as the upper limit and the lower limit of the distance interval. In this way, the distance interval of each type of normal light spot is obtained. If the fluorescence brightness of a light spot does not belong to the fluorescence brightness interval of each type of normal light spot, or the fluorescence brightness of this light spot does not belong to the distance interval of each type of normal light spot, it indicates that this light spot is a specific light spot. If a light spot of a certain solution is a specific light spot, then this solution is recorded as an abnormal solution. The fluorescence brightness interval of each type of specific light spot is obtained from the database. If the fluorescence brightness of a light spot of an abnormal solution belongs to the fluorescence brightness interval of a certain type of specific light spot, it indicates that this abnormal solution has the abnormal body corresponding to this specific light spot. In this way, the abnormal bodies of each abnormal solution are obtained.

[0026] It should be noted that the abnormal body is the object of fluorescence immunoassay chromatography test. For example, viruses or bacteria, etc. The number of the abnormal body is g. The abnormal body can bind to the corresponding antibody on the fixed plate, and the conjugate can react with the fluorescent reagent to produce fluorescence. The light spot is the conjugate of the fluorescent-labeled abnormal body and the corresponding antibody. When the test solution contains any abnormal body, it indicates that the test solution is an abnormal solution. For example, the fluorescence brightness interval of the g antibody corresponding to the specific light spot is 6 - 8. If the brightness interval of the light spot c of the a solution is 7, it indicates that the a solution contains the abnormal body g corresponding to the antibody, and further indicates that the a solution is an abnormal solution.

[0027] Step 2: Specific chromatography test: According to the abnormal bodies of each abnormal solution, set the initial specific chromatography test, collect the specific test data of each standard concentration of each abnormal solution, and then judge whether it is necessary to dilute the specific chromatography test. When performing the diluted specific chromatography test, collect the specific test data of each diluted solution of each abnormal solution. According to the solution specific model, analyze the specific test data of each diluted solution of each abnormal solution to obtain the types and concentrations of the abnormal bodies of each abnormal solution.

[0028] In a specific embodiment, the initial specific chromatography test is set as follows: Each abnormal solution is diluted at a preset dilution ratio to obtain a standard concentration dilution solution of each abnormal solution. According to each abnormal substance in each abnormal solution, a corresponding antigen fixing plate is selected to obtain various antigen fixing plates for each abnormal solution. The standard concentration dilution solution of each abnormal solution is allowed to flow into the corresponding various antigen fixing plates to obtain various antigen fluorescence images of each abnormal solution.

[0029] In a specific embodiment, the determination of whether dilution of the specific chromatography test is required is as follows: The specific test data of the standard concentration of each abnormal solution includes the fluorescence brightness and distance of each light spot in each antigen fluorescence image of each standard concentration abnormal solution. If the fluorescence brightness of any light spot in a certain antigen fluorescence image belongs to the fluorescence brightness interval corresponding to the antigen, and the distance of the light spot belongs to the distance interval corresponding to the antigen, it indicates that the antigen fluorescence image has a specific light spot. If a certain antigen fluorescence image of a certain abnormal solution has a specific light spot, it indicates that the standard concentration abnormal solution has the abnormal substance. If the abnormal substances of the standard concentration dilution solution of a certain abnormal solution are the same as those of the abnormal solution, it indicates that the initial specific chromatography test of the abnormal solution is accurate and there is no need to dilute the specific chromatography test. The sizes of the specific light spots of each solution with accurate initial specific chromatography test are collected, and the sizes of the specific light spots of each solution with accurate initial specific chromatography test are substituted into the concentration calculation formula to obtain the concentration of abnormal substance b in abnormal solution a , where a is the number of the abnormal solution, , , where b is the abnormal substance, , , and are respectively the light spot size and the mobile phase wetting test paper size of the initial specific chromatography test of abnormal substance b in abnormal solution a, is the preset concentration correction factor, and are respectively the volume and mass of the solution used in the initial specific chromatography test of abnormal substance b in abnormal solution a, and are respectively the volume of the solution after dilution of abnormal solution a at the preset dilution ratio and the volume of the solution before dilution.

[0030] It should be noted that the concentration correction factor is set by the staff, for example, 1.5.

[0031] In a specific embodiment, the dilution-specific chromatography test is carried out as follows: Dilute each abnormal solution at various dilution ratios to obtain diluted solutions of each abnormal solution, and perform a specific chromatography test to obtain various antigen fluorescence images of the diluted solutions of each abnormal solution. Obtain the fluorescence brightness and distance of each light spot from the various antigen fluorescence images, and make a judgment based on the fluorescence brightness and distance of each light spot on the various antigen fluorescence images of the diluted solutions of each abnormal solution, so as to obtain each abnormal body and the light spot size corresponding to each abnormal body of the diluted solutions of each abnormal solution.

[0032] In a specific embodiment, the analysis of the specific test data of the diluted solutions of each abnormal solution is as follows: The specific test data of the diluted solutions of each abnormal solution include each abnormal body and the light spot size corresponding to each abnormal body of the diluted solutions of each abnormal solution. Count each abnormal body of the diluted solutions of each abnormal solution to obtain the occurrence times of each abnormal body of each abnormal solution. Substitute the sizes of the specific light spots of the diluted solutions of each solution into the calculation formula to obtain the concentration of abnormal body b in the c-diluted solution of abnormal solution a, where c is the number of the diluted solution. , , and are respectively the light spot size and the mobile phase wetting test paper size for the dilution-specific chromatography test of abnormal body b in the c-diluted solution of abnormal solution a. and are the volume of the solution used and the mass of the solution used for the dilution-specific chromatography test of abnormal body b in the c-diluted solution of abnormal solution a. and are respectively the volume of the diluted solution and the volume of the solution before dilution corresponding to the c-diluted solution of abnormal solution a. is the light spot size tested for abnormal body b in the c-diluted solution of abnormal solution a. is the volume of the solution used tested for abnormal body b in the c-diluted solution of abnormal solution a. and are respectively the volume of the diluted solution and the volume of the solution before dilution of the c-diluted solution of abnormal solution a. Obtain the standard concentration corresponding to each abnormal body from the database. If the concentration of a certain abnormal body in a certain diluted solution of a certain solution is greater than the standard concentration, it indicates that the concentration of this abnormal body in this diluted solution of this solution is a high concentration, and count the occurrence times of high concentrations of each abnormal body of each abnormal solution.

[0033] Input the occurrence times of each abnormal body and the occurrence times of high concentration in each abnormal solution into the solution-specific model to obtain the output results of each abnormal solution. If the output result of each abnormal solution is 1, it indicates that the dilution-specific test of this abnormal body in this abnormal solution is accurate. If the output result is 0, it indicates that the dilution-specific test of this abnormal body in this abnormal solution is inaccurate, and replace the dilution solution and continue the dilution-specific chromatography test.

[0034] In a specific embodiment, the specific setting process of the solution-specific model is as follows: Input the occurrence times of each abnormal body and the occurrence times of high concentration in each abnormal solution into the solution-specific model: to obtain the output results of each abnormal solution , where a is the abnormal solution number, , , where b is the abnormal body, , , and are respectively the occurrence times of abnormal body b in abnormal solution a and the occurrence times of high concentration. A and B are respectively the preset standard occurrence times of abnormal bodies and the occurrence times of high concentration. and are respectively the weight factors of the preset standard occurrence times of abnormal bodies and the weight factors of the occurrence times of high concentration. , , , where C is the standard test accuracy index.

[0035] It should be noted that the preset standard occurrence times of abnormal bodies and the occurrence times of high concentration are set by the staff. For example, the standard occurrence times of abnormal bodies are 8 times and the occurrence times of high concentration are 1 time. The weight factors of the preset standard occurrence times of abnormal bodies and the weight factors of the occurrence times of high concentration are set by the staff. For example, the weight factor of the standard occurrence times of abnormal bodies is 0.3 and the weight factor of the occurrence times of high concentration is 0.7.

[0036] In a specific embodiment, the analysis of the solution basic data, the types of abnormal bodies and the concentrations of abnormal bodies in each abnormal solution is as follows: The solution basic data includes the standard concentration and the maximum concentration corresponding to each abnormal body. Input the standard concentration corresponding to each abnormal body into the solution abnormality model to obtain the output results of each solution. If the output result of a certain solution is 1 and the concentrations of all abnormal bodies in this solution are less than the corresponding maximum concentrations of each abnormal body, it indicates that this solution meets the requirements. If the output result of a certain solution is 0, or the concentration of a certain abnormal body in this solution is greater than or equal to the corresponding maximum concentration of each abnormal body, it indicates that this solution is abnormal and a warning is given.

[0037] In a specific embodiment, the specific setting process of the solution abnormality model is as follows: Input the standard concentration corresponding to each abnormal body into the solution abnormality model: , the output result of the abnormal solution a is obtained , and are respectively the concentration of the abnormal body b of the abnormal solution a and the standard concentration corresponding to the preset abnormal body b. a is the abnormal solution number, , , b is the abnormal body, , , is the weight factor of the preset concentration of the abnormal body b, , , N is the abnormal index of the standard solution.

[0038] It should be noted that the standard concentrations corresponding to the preset abnormal bodies are set by the staff. For example, the standard concentration corresponding to an abnormal body is 10000 cfu / g, and the weight factors of the preset concentrations of the abnormal bodies are set by the staff. For example, the weight factor of the concentration of an abnormal body is 0.08.

[0039] The above content is only an example and explanation of the concept of the present invention. Those skilled in the art of this technology make various modifications or supplements to the described specific embodiments or use similar methods to replace them, as long as they do not deviate from the concept of the invention or exceed the scope defined by this specification, they should all fall within the protection scope of the present invention.

Claims

1. A method for processing fluorescent immunochromatography test data, characterized in that: The steps include: Step 1, basic chromatography test: perform basic chromatography test on each test object to obtain fluorescence images of each solution, and then collect basic chromatography information of each solution from the fluorescence images of each solution, analyze the basic chromatography information of each solution to obtain each abnormal solution, and identify each abnormal body of each abnormal solution from each abnormal solution; Step 2, specific chromatography test: according to each abnormal body of each abnormal solution, set the initial specific chromatography test, collect each specific test data of the standard concentration of each abnormal solution, and then determine whether the dilution specific chromatography test is needed. When the dilution specific chromatography test is performed, collect each specific test data of each diluted solution of each abnormal solution, and analyze each specific test data of each diluted solution of each abnormal solution according to the solution-specific model to obtain the type and concentration of the abnormal body of each abnormal solution; Step 3: Chromatographic data analysis: Obtain solution basic data from the database, analyze the solution basic data, the abnormal body type and abnormal body concentration of each abnormal solution according to the solution abnormality model, and determine whether an early warning is needed; The specific judgment process of judging whether it is necessary to dilute the specific chromatography test is as follows: each specific test data of the standard concentration of each abnormal solution includes the fluorescence brightness and distance of each light spot in each antigen fluorescence image of each standard concentration abnormal solution. If the fluorescence brightness of any light spot in a certain antigen fluorescence image belongs to the fluorescence brightness interval corresponding to the antigen, and the distance of the light spot belongs to the distance interval corresponding to the antigen, it indicates that the antigen fluorescence image has a specific light spot. If a certain antigen fluorescence image of a certain abnormal solution has a specific light spot, it indicates that the standard concentration abnormal solution has the abnormal body. If the abnormal bodies of the standard concentration diluted solution of a certain abnormal solution are the same as the abnormal bodies of the abnormal solution, it indicates that the initial specific chromatography test of the abnormal solution is accurate and there is no need to dilute the specific chromatography test. The sizes of each specific light spot of each solution accurately obtained by the initial specific chromatography test are collected, and the sizes of each specific light spot of each solution accurately obtained by the initial specific chromatography test are substituted into the concentration calculation formula to obtain the concentration of each abnormal body of each solution. The specific test data of each dilution of each abnormal solution are analyzed, and the specific analysis results are as follows: the specific test data of each dilution of each abnormal solution include each abnormal body of each dilution of each abnormal solution and the spot size corresponding to each abnormal body, each abnormal body of each dilution of each abnormal solution is counted to obtain the number of occurrences of each abnormal body of each abnormal solution, the size of each specific spot of each dilution of each solution is substituted into the calculation formula to obtain the concentration of each abnormal body of each dilution of each abnormal solution, and the standard concentration corresponding to each abnormal body is obtained from the database. If the concentration of a certain abnormal body of a certain dilution of a certain solution is greater than the standard concentration, it indicates that the concentration of the abnormal body of the dilution of the solution is high concentration, and the number of occurrences of high concentration of each abnormal body of each abnormal solution is obtained by statistics; The number of occurrences of each abnormal body and the number of occurrences of high concentration of each abnormal solution are input into the solution-specific model to obtain the output result of each abnormal solution. If the output result of each abnormal solution is 1, it indicates that the dilution-specific test of the abnormal body of the abnormal solution is accurate. If the output result is 0, it indicates that the dilution-specific test of the abnormal body of the abnormal solution is inaccurate, and the dilution solution is replaced to continue the dilution-specific chromatography test; The specific setting process of the solution-specific model is as follows: the number of occurrences of each abnormal body and the number of occurrences of high concentrations of each abnormal solution are input into the solution-specific model: Get the output result α of each abnormal solution ab , a is the number of the abnormal solution, a=1,2......q, q>2, b is the abnormal body, b=1,2......p, p>2, A ab and B ab are the number of occurrences of abnormal body b and the number of occurrences of high concentration of abnormal solution a, respectively; A and B are the number of occurrences of the preset standard abnormal body and the number of occurrences of high concentration, respectively; ε1 and ε2 are the weight factors of the preset number of occurrences of the standard abnormal body and the number of occurrences of high concentration, respectively; ε1>0, ε2>0, ε1+ε2=1, and C is the standard test accuracy index; The basic data of the solution, the type of abnormal body and the concentration of the abnormal body of each abnormal solution are analyzed. The specific analysis process is as follows: the basic data of the solution includes the standard concentration and maximum concentration corresponding to each abnormal body. The standard concentration corresponding to each abnormal body is input into the solution abnormal model to obtain the output result of each solution. If the output result of a solution is 1, and the concentration of each abnormal body of the solution is less than the corresponding maximum concentration of each abnormal body, it indicates that the solution meets the requirements. If the output result of a solution is 0, or the concentration of a certain abnormal body of the solution is greater than or equal to the corresponding maximum concentration of each abnormal body, it indicates that the solution is abnormal and a warning is issued; The specific setting process of the solution anomaly model is as follows: input the standard concentration corresponding to each anomaly into the solution anomaly model: Get the output result of a abnormal solution β a , M ab and M′ b are the concentration of the abnormal substance b of the abnormal solution a and the preset standard concentration corresponding to the abnormal substance b, a is the abnormal solution number, a=1,2......q, q>2, b is the abnormal substance, b=1,2......p, p>2, φ b is the weight factor of the preset abnormal body b concentration, φ b >0, N is the abnormal index of the standard solution; The basic chromatographic information of each solution includes the distance and fluorescence brightness of each light spot in each solution.

2. The method for processing fluorescent immunochromatography test data according to claim 1, characterized in that: The basic chromatography information of each solution is collected, and the specific collection process is as follows: The types of each analyte are obtained from a database, and a fixed plate for detecting antigens is selected according to the types of each analyte, each analyte is made into a solution, and then a part of the solution of each analyte is washed into the fixed plate, and after chromatography, a fluorescence image of each basic chromatography solution is obtained through a fluorescence observation device, and basic chromatography information of each solution is obtained from the fluorescence image of each basic chromatography solution; The fluorescence image of each basic chromatography solution is obtained by fluorescence recognition technology to obtain the distance and fluorescence brightness of each light spot to the test line. The shortest distance from each light spot to the test line is selected and recorded as the distance of each light spot, thereby obtaining the distance and fluorescence brightness of each light spot in each solution.

3. The method for processing fluorescent immunochromatography test data according to claim 2, characterized in that: The basic chromatography information of each solution is analyzed, and the specific analysis process is as follows: The fluorescence brightness and distance of each light spot of the standard solution are obtained from the database, and the fluorescence brightness and distance of each light spot of the standard solution are substituted into the calculation formula to obtain the brightness deviation value and the distance deviation value between each light spot of the standard solution. If the brightness deviation value of a pair of light spots of the standard solution is less than the preset light spot deviation value and the distance deviation value is less than the preset distance deviation value, it indicates that the pair of light spots of the standard solution are the same type of normal light spots, thereby obtaining various types of normal light spots, and the fluorescence brightness and distance of each light spot of the standard solution are statistically analyzed to obtain each fluorescence brightness and each distance of each type of normal light spot; The maximum fluorescence brightness and the minimum fluorescence brightness of each type of normal light spot are recorded as the upper limit and the lower limit of the fluorescence brightness interval, respectively, to obtain the fluorescence brightness interval of each type of normal light spot. The shortest distance and the minimum distance of each type of normal light spot are recorded as the upper limit and the lower limit of the distance interval, respectively, to obtain the distance interval of each type of normal light spot. If the fluorescence brightness of a certain light spot does not belong to the fluorescence brightness interval of various types of normal light spots, or the fluorescence brightness of the light spot does not belong to the distance interval of various types of normal light spots, it indicates that the light spot is a specific light spot. If a certain light spot of a certain solution is a specific light spot, then the solution is recorded as an abnormal solution, and the fluorescence brightness interval of each type of specific light spot is obtained from the database. If the fluorescence brightness of a certain light spot of an abnormal solution belongs to the fluorescence brightness interval of a certain type of specific light spot, it indicates that the abnormal solution has an abnormal body corresponding to the specific light spot, thereby obtaining each abnormal body of each abnormal solution.

4. The method for processing fluorescent immunochromatography test data according to claim 1, characterized in that: The initial specific chromatography test is set up, and the specific setting process is as follows: Each abnormal solution is diluted at a preset dilution ratio to obtain a dilution solution of a standard concentration of each abnormal solution. A corresponding antigen fixing plate is selected according to each abnormal body of each abnormal solution to obtain various antigen fixing plates of each abnormal solution. The dilution solution of the standard concentration of each abnormal solution is flowed into the corresponding various antigen fixing plates to obtain various antigen fluorescence images of each abnormal solution.

5. The method for processing fluorescent immunochromatography test data according to claim 1, characterized in that: The dilution specific chromatography test is performed, and the specific test process is as follows: Each abnormal solution is diluted at a different dilution ratio to obtain each diluted solution of each abnormal solution, and a specific chromatography test is performed to obtain various antigen fluorescence images of each diluted solution of each abnormal solution, and the fluorescence brightness and distance of each light spot are obtained from the various antigen fluorescence images. According to the fluorescence brightness and distance of each light spot on the various antigen fluorescence images of each diluted solution of each abnormal solution, judgment is made to obtain each abnormal body of each diluted solution of each abnormal solution and the spot size corresponding to each abnormal body.

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