Preparation method of Chinese herbal fermented eye drops

Abstract

The present invention relates to a method for preparing Chinese herbal fermented eye drops, specifically, the method for preparing the Chinese herbal fermented eye drops comprises: a Chinese herbal medicine washing step, mixing a Chinese herbal medicine mixture, vinegar and crude salt in pure water and placing, and then washing; a heating step, putting the Chinese herbal medicine mixture washed in the Chinese herbal medicine washing step into a decoction machine and mixing with pure water for heating; a cooling and fermenting step, cooling the decoction prepared in the heating step and then fermenting; an eye drop raw material extraction step, extracting the eye drop raw material by distilling the fermented product prepared in the cooling and fermenting step; and an alkaline water mixing step, mixing alkaline water in the eye drop raw material prepared in the eye drop raw material extraction step. The Chinese herbal fermented eye drops prepared by the process do not contain chemical components and have low cytotoxicity, so they are safe for the human body and show excellent bactericidal effects, thereby showing the effect of improving eye diseases such as inflammation or congestion.

Description

Technical Field

[0001] The invention relates to a method for preparing a Chinese herbal fermented eye medicine. The Chinese herbal fermented eye medicine does not contain chemical components and has low cytotoxicity, so it is safe for the human body and exhibits excellent bactericidal effect, thereby exhibiting the effect of improving eye diseases such as inflammation or congestion. Background Art

[0002] Modern people are suffering from eye diseases caused by excessive consumption of blood and qi in the eyes due to the use of various electronic devices. In particular, excessive use of smartphones and computers can lead to chronic diseases such as dry eyes, and more and more people are using eye drops to improve this disease.

[0003] In addition, excessive pressure can cause blood circulation disorders around the eyes and in the eyes themselves, leading to inflammation or damage to retinal cells, which can lead to intractable eye diseases such as glaucoma or macular degeneration, and in the worst case even blindness.

[0004] There are more than thousands of eye drops used for dry eye worldwide, but most use synthetic chemicals or chemical drugs. Eye drops using these chemicals may help relieve short-term symptoms, but have little effect on improving blood flow to the eye itself or treating the conjunctiva or retina.

[0005] Under such circumstances, in order to develop eye drops made from natural Chinese medicinal materials and apply them clinically, based on the basic principles of traditional Chinese medicine, attempts are being made to develop eye drops using Chinese medicinal materials whose stability has been clinically confirmed to be safe for thousands of years.

[0006] Prior art literature

[0007] Patent Literature

[0008] (Patent Document 1) Korean Patent No. 10-1740869 (May 22, 2017)

[0009] (Patent Document 2) Korean Patent No. 10-2002612 (July 16, 2019) Summary of the invention

[0010] Technical issues

[0011] The object of the present invention is to provide a method for preparing a Chinese herbal fermented eye medicine, which does not contain chemical components and has low cytotoxicity, is safe for the human body and exhibits excellent bactericidal effects, thereby showing the effect of improving eye diseases such as inflammation or congestion.

[0012] Technical Solution

[0013] The object of the present invention is achieved by providing a method for preparing Chinese herbal fermented eye drops, which is characterized in that the preparation method comprises: a Chinese medicinal material washing step, mixing the Chinese medicinal material mixture, vinegar and crude salt in pure water and placing them, and then washing; a heating step, putting the Chinese medicinal material mixture washed in the Chinese medicinal material washing step into a decoction machine and mixing them with pure water for heating; a cooling and fermenting step, cooling the decoction prepared in the heating step and then fermenting it; an eye drop raw material extraction step, extracting the eye drop raw material by distilling the fermented material prepared in the cooling and fermentation step; and an alkaline water mixing step, mixing alkaline water with the eye drop raw material prepared in the eye drop raw material extraction step, wherein the Chinese medicinal material mixture consists of wolfberry, rehmannia, shiitake mushroom, ganoderma lucidum, mulberry leaf, mulberry root, deer antler, ginseng, astragalus, tangerine peel, chamomile, dandelion, white cocklebur, dodder seed, onion, purslane, millennium grass, ginger, jujube and licorice.

[0014] According to a preferred feature of the present invention, the Chinese medicinal materials washing step comprises: mixing 10 to 15 parts by weight of a Chinese medicinal materials mixture, 1 to 2 parts by weight of vinegar and 0.2 to 0.4 parts by weight of coarse salt in 100 parts by weight of pure water, and leaving it for 4 to 6 minutes, and then washing the Chinese medicinal materials.

[0015] According to a more preferred feature of the present invention, the heating step includes: mixing 400 to 600 parts by weight of pure water relative to 100 parts by weight of the Chinese medicinal material mixture washed in the Chinese medicinal material washing step, and heating at a temperature of 100°C to 120°C for 8 to 10 hours, and then heating at a temperature of 80°C to 90°C for 10 to 15 hours.

[0016] According to a more preferred feature of the present invention, the cooling fermentation step comprises: cooling the decoction prepared by the heating step to a temperature of 40°C to 45°C, then inoculating 0.01 to 0.1 parts by weight of the fermentation strain into 100 parts by weight of the cooled decoction, and fermenting for 14 to 16 hours.

[0017] According to a further preferred feature of the present invention, the fermentation strain is formed by mixing Lactobacillus acidophilus, Lactobacillus bulgaricus, Lactobacillus paracasei, Bifidobacterium breve and Lactobacillus rhamnosus in a weight ratio of 1:1:1:1:1.

[0018] According to a further preferred feature of the present invention, the alkaline water mixing step is performed by mixing 0.05 to 0.15 parts by weight of the eye medicine raw material prepared in the eye medicine raw material extraction step with 100 parts by weight of alkaline water.

[0019] According to a further preferred feature of the present invention, the pH value of the alkaline water is 7.2 to 7.6.

[0020] Beneficial Effects

[0021] The preparation method of the Chinese herbal fermented eye drops of the present invention provides a Chinese herbal fermented eye drops, which contain no chemical components and have low cytotoxicity, are therefore safe for the human body and exhibit excellent bactericidal effects, thereby exhibiting the effect of improving eye diseases such as inflammation or congestion. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 The present invention is a flow chart showing the method for preparing the fermented Chinese herbal medicine eye drops.

[0023] Figure 2 This is a photograph showing the distillation extraction apparatus used in the present invention.

[0024] Reference numerals:

[0025] S101: Chinese medicinal materials washing steps

[0026] S103: Heating step

[0027] S105: Cooling and fermentation step

[0028] S107; step of extracting raw materials of eye drops

[0029] S109: Alkaline water mixing step DETAILED DESCRIPTION

[0030] The preferred embodiments of the present invention and the physical properties of each component are described in detail below. This is a detailed description to enable ordinary technicians in the technical field to which the present invention belongs to easily implement the present invention, and it does not mean that the technical concept and scope of the present invention are limited thereby.

[0031] The preparation method of the Chinese herbal fermented eye drops of the present invention comprises: a Chinese herbal medicine washing step S101, mixing the Chinese herbal medicine mixture, vinegar and crude salt in pure water and placing them, and then washing; a heating step S103, putting the Chinese herbal medicine mixture washed in the Chinese herbal medicine washing step into a medicine decocting machine and mixing with pure water for heating; a cooling and fermentation step S105, cooling the decoction prepared in the heating step S103 and then fermenting it; an eye drop raw material extraction step S107, extracting the eye drop raw material by distilling the fermented product prepared in the cooling and fermentation step S105; and an alkaline water mixing step S109, mixing alkaline water with the eye drop raw material prepared in the eye drop raw material extraction step S107.

[0032] The Chinese medicinal material washing step S101 is a step of mixing the Chinese medicinal material mixture, vinegar and coarse salt in pure water and placing them, and then washing, preferably comprising: mixing 10 to 15 parts by weight of the Chinese medicinal material mixture, 1 to 2 parts by weight of vinegar and 0.2 to 0.4 parts by weight of coarse salt in 100 parts by weight of pure water, and placing them for 4 to 6 minutes, and then separating the Chinese medicinal material mixture in the mixture, and rubbing the separated Chinese medicinal material mixture against each other for washing.

[0033] Through the process, foreign matter remaining on the surface of the Chinese medicinal materials is removed. At this time, the Chinese medicinal material mixture is preferably composed of wolfberry, rehmannia, shiitake mushrooms, ganoderma lucidum, mulberry leaves, mulberry roots, deer antlers, ginseng, astragalus, tangerine peel, chamomile, dandelion, white tribulus, dodder seed, onion, purslane, safflower, ginger, jujube and licorice, and more preferably wolfberry, rehmannia, shiitake mushrooms, ganoderma lucidum, mulberry leaves, mulberry roots, deer antlers, ginseng, astragalus, tangerine peel, chamomile, dandelion, white tribulus, dodder seed, onion, purslane, safflower, ginger, jujube and licorice are mixed in equal weight parts.

[0034] The mulberry leaves and roots, shiitake mushrooms and ganoderma lucidum have the function of providing nutrients required by the eyes.

[0035] In addition, the wolfberry and rehmannia root not only provide the nutrients needed by the eyes, but also have the function of repairing damaged eye cells, repairing conjunctival and retinal cells damaged by excessive eye use, thereby improving diseases such as dry eye or macular degeneration.

[0036] In addition, the white tribulus, dandelion and chamomile have the effect of improving conjunctivitis or optic neuritis caused by inflammation due to insufficient blood flow to the eyes.

[0037] In addition, the pilose antler, ginseng, astragalus and tangerine peel have the effect of promoting the circulation of qi and blood, and the onion and purslane have the effect of improving eyeball congestion by eliminating blood stasis.

[0038] The heating step S103 is a step of putting the Chinese medicinal material mixture washed in the Chinese medicinal material washing step S101 into a medicine decocting machine and mixing it with pure water for heating, preferably comprising: putting 100 parts by weight of the Chinese medicinal material mixture washed in the Chinese medicinal material washing step S101 into a medicine decocting machine and mixing it with 400 to 600 parts by weight of pure water, and heating it at a temperature of 100°C to 120°C for 8 to 10 hours, and then heating it at a temperature of 80°C to 90°C for 10 to 15 hours.

[0039] In the heating step S103, if the content of pure water is less than 400 parts by weight, the amount of pure water is excessively reduced during the heating process, resulting in the inability to extract the effective ingredients contained in the Chinese medicinal material mixture normally. In the heating step S103, if the content of pure water exceeds 600 parts by weight, the concentration of the prepared decoction is too low and requires an additional concentration process, so it is not the preferred option.

[0040] In addition, if the initial heating temperature of the heating step S103 is lower than 100°C or the heating time is less than 8 hours, the above-mentioned effect is negligible. When the initial heating temperature of the heating step exceeds 120°C or the heating time exceeds 10 hours, the dissolution effect of the active ingredients contained in the Chinese medicinal material mixture will not be significantly improved and the extracted active ingredients will be destroyed or it is not the preferred option from the perspective of energy efficiency.

[0041] The cooling and fermentation step S105 is a step of fermenting the decoction prepared by the heating step S103 after cooling it, and preferably includes: cooling the decoction prepared by the heating step S103 to a temperature of 40°C to 45°C, then inoculating 0.01 to 0.1 parts by weight of the fermentation strain into 100 parts by weight of the cooled decoction, and fermenting for 14 to 16 hours.

[0042] When undergoing the fermentation process including the above process, the effective ingredients extracted in the mixture of traditional Chinese medicines are refined into small particles of nanometer size, so that the absorption rate in the body is significantly improved.

[0043] At this time, the fermentation strain is preferably a mixture of Lactobacillus acidophilus, Lactobacillus bulgaricus, Lactobacillus paracasei, Bifidobacterium breve and Lactobacillus rhamnosus in a weight ratio of 1:1:1:1:1, wherein the fermentation strain composed of the strains provides assistance to each other in the parts required by the natural medicinal materials during the fermentation of the natural medicinal materials, thereby improving the fermentation efficacy of the natural medicinal materials used in the preparation of eye drops.

[0044] If the temperature of the cooling fermentation step S105 is lower than 40°C or the fermentation time is less than 14 hours, the effect will be minimal due to the low efficiency of the fermentation process. If the temperature of the cooling fermentation step S105 exceeds 45°C or the fermentation time exceeds 16 hours, anaerobic fermentation will be carried out while the effect may not be significantly improved, so it is not a preferred option.

[0045] The eye medicine raw material extraction step S107 is a step of extracting the eye medicine raw material by distilling the fermented product prepared by the cooling fermentation step S105, and is achieved by putting the fermented product prepared by the cooling fermentation step S105 into a distillation device and performing a distillation extraction process, wherein the distillation extraction conditions performed in the eye medicine raw material extraction step S107 performed in the present invention can be performed under conventional distillation extraction conditions.

[0046] In addition, any distillation extraction device used in the ophthalmic drug raw material extraction step S107 of the present invention can be used without particular limitation as long as it is a device capable of distilling and extracting the ophthalmic drug active ingredient contained in the fermented product prepared in the cooling and fermentation step S105 as described above, but it is preferably used as follows: Figure 2 The distillation extraction apparatus shown.

[0047] The alkaline water mixing step S109 is a step of mixing alkaline water with the ophthalmic raw material prepared in the ophthalmic raw material extraction step S107, preferably mixing 0.05 to 0.15 parts by weight of the ophthalmic raw material prepared in the ophthalmic raw material extraction step S107 with 100 parts by weight of alkaline water.

[0048] At this time, the alkaline water preferably uses alkaline water having a pH value of 7.2 to 7.6, and the alkaline water used in the present invention may be alkaline water prepared by using the "electrolytic water purifier" described in Korean Patent No. 10-0419536.

[0049] Eye drops using alkaline water prepared by the electrolytic water purifier as described above show a pH value similar to that of tears, so they are comfortable for the eyes and do not produce rejection reactions. If the pH value of the alkaline water is lower than 7.2, the pH value is too low compared to tears, so it is not a preferred option. If the pH value of the alkaline water exceeds 7.6, the pH value of the prepared eye drops is too high, which may increase irritation to the eyes, so it is not a preferred option.

[0050] In addition, if the content of the ophthalmic raw materials mixed in the alkaline water mixing step S109 is less than 0.05 parts by weight, the effect of inhibiting early eye diseases or improving eye diseases (such as inflammation or congestion, etc.) is negligible due to the low bactericidal effect. If the content of the ophthalmic raw materials exceeds 0.15 parts by weight, the effect cannot be significantly improved and the cytotoxicity or irritation to the eyes is excessively increased. Therefore, it is not a preferred option.

[0051] After the alkaline water mixing step S109, the preparation of the Chinese herbal fermented eye drops of the present invention is completed. The Chinese herbal fermented eye drops prepared by the present invention may contain one or more ingredients selected from anti-inflammatory agents (allantoin, pranoprofen, etc.), anti-inflammatory agents (naphazoline hydrochloride, etc.), ocular regulators (neostigmine methylsulfate, etc.), astringents (zinc sulfate, etc.), antihistamines (chlorpheniramine maleate, diphenhydramine hydrochloride, etc.), antiallergic agents (sodium cromoglycate, etc.), vitamins (tocopherol acetate, sodium flavin adenine dinucleotide, pyridoxine hydrochloride, cyanocobalamin and panthenol, etc.), sulfonamides (sulfamethoxazole, etc.), chondroitin sulfate and amino acids, etc., without affecting the intended effect.

[0052] In addition, the Chinese herbal fermented eye medicine prepared by the present invention is particularly suitable for eye drops and eye ointments among the dosage forms including eye drops, eye ointments, powders, granules, tablets, capsules, injections, etc., and the preparations of such dosage forms can be prepared by conventional methods.

[0053] The aqueous solution and diluent for the suspension used in the eye drops are distilled water and physiological saline, and the non-aqueous solution and diluent for the suspension are vegetable oil, liquid paraffin, mineral oil, propylene glycol, octyldodecanol, and the like.

[0054] In addition, the ophthalmic composition of the present invention can be used to prevent or treat the above diseases or symptoms in humans and non-human animals (eg, non-human mammals (such as pigs, cows, horses, dogs, cats and other livestock and pets)).

[0055] Hereinafter, the preparation method of the fermented Chinese herbal medicine eye drops of the present invention and the physical properties of the fermented Chinese herbal medicine eye drops prepared by the preparation method will be described through examples.

[0056] <Preparation Example 1> Preparation of Chinese herbal medicine mixture

[0057] 800 g of a Chinese medicinal material mixture was prepared by mixing 40 g of wolfberry, 40 g of rehmannia, 40 g of shiitake mushroom, 40 g of ganoderma lucidum, 40 g of mulberry leaf, 40 g of mulberry root, 40 g of pilose antler, 40 g of ginseng, 40 g of astragalus, 40 g of tangerine peel, 40 g of chamomile, 40 g of dandelion, 40 g of tribulus terrestris, 40 g of dodder seed, 40 g of onion, 40 g of purslane, 40 g of scutellaria baicalensis, 40 g of ginger, 40 g of jujube and 40 g of licorice.

[0058] <Preparation Example 2> Preparation of fermentation strain

[0059] Lactobacillus acidophilus, Lactobacillus bulgaricus, Lactobacillus paracasei, Bifidobacterium breve and Lactobacillus rhamnosus were mixed in a weight ratio of 1:1:1:1:1 to prepare a fermentation strain.

[0060] <Example 1>

[0061] 800 g of the Chinese medicinal material mixture prepared by the preparation example 1, 90 mL of vinegar and 20 g of crude salt were mixed in 6 L of pure water and left for 5 minutes. The Chinese medicinal material mixture was separated and rubbed, and then washed through 3 rinsing processes. The washed Chinese medicinal material mixture was put into a non-woven bag for decocting medicine and put into a decocting machine. Then 2 L of pure water was added to the decocting machine, and heated at 110° C. for 9 hours, and then heated at 85° C. for an additional 12 hours to extract the decoction. After the extracted decoction was cooled to 42° C., 0.05 weight parts of the fermentation strain prepared by the preparation example 2 was mixed with 100 weight parts of the cooled decoction and fermented for 15 hours. The fermented fermented liquid was put into a distillation extractor for distillation extraction to prepare an eye medicine raw material, and 0.1 weight parts of the prepared eye medicine raw material was mixed with 100 weight parts of alkaline water (pH value is 7.4), thereby preparing Chinese herbal fermented eye medicine.

[0062] <Example 2>

[0063] The same method as in Example 1 was followed and 0.05 parts by weight of the ophthalmic raw materials were mixed to prepare a Chinese herbal fermented ophthalmic drug.

[0064] <Example 3>

[0065] The same method as in Example 1 was followed and 0.15 parts by weight of the eye medicine raw materials were mixed to prepare a Chinese herbal fermented eye medicine.

[0066] <Comparative Example 1>

[0067] 800 g of the Chinese medicinal material mixture prepared by the preparation example 1, 90 mL of vinegar and 20 g of crude salt were mixed in 6 L of pure water and left for 5 minutes. The Chinese medicinal material mixture was separated and rubbed, and then washed through 3 rinsing processes. The washed Chinese medicinal material mixture was put into a non-woven bag for decocting medicine and put into a decocting machine. Then 2 L of pure water was added to the decocting machine, and heated at 110°C for 9 hours. Then, it was heated at 85°C for an additional 12 hours to extract the decoction. After the extracted decoction was cooled to 42°C, the cooled decoction was put into a distillation extractor for distillation extraction, thereby preparing an eye medicine raw material, and 0.1 weight part of the prepared eye medicine raw material was mixed with 100 weight parts of alkaline water (pH value is 7.4), thereby preparing eye medicine.

[0068] <Comparative Example 2>

[0069] The same manner as in Example 1 was carried out, and an eye drug was prepared by mixing 0.01 parts by weight of the eye drug raw materials.

[0070] <Comparative Example 3>

[0071] The same manner as in Example 1 was carried out, and an eye drug was prepared by mixing 1.0 parts by weight of the eye drug raw materials.

[0072] The eye drops prepared by Examples 1 to 3 and Comparative Examples 1 to 3 were measured for improving eyeball inflammation and eyeball congestion, and the results are shown in Table 1 below.

[0073] Among them, the improvement of ocular inflammation and ocular congestion was carried out on 60 subjects with ocular inflammation and 60 subjects with ocular hemorrhage, and the subjects were divided into 6 groups, 10 in each group, and the prepared eye drops were administered every 6 hours, 3 times a day, 0.45 mL each time. After 2 days of administration, the improvement effects of ocular inflammation and ocular congestion were investigated using a 5-point scale method, and the method of showing the average value was used.

[0074] 5 points: good improvement; 4 points: improvement; 3 points: fair; 2 points: deterioration; 1 point: severe deterioration.

[0075]

[0076] distinguish Eye inflammation Bloodshot eyes Example 1 4.5 4.4 Example 2 4.2 4.3 Example 3 4.6 4.5 Comparative Example 1 4.0 3.9 Comparative Example 2 3.4 3.2 Comparative Example 3 4.6 4.4

[0077] As shown in Table 1, the eye drops prepared by Examples 1 to 3 of the present invention have excellent effects of improving eyeball inflammation and eyeball congestion compared to the eye drops prepared by Comparative Examples 1 to 2. In particular, even if the same components are mixed, the effects of improving eyeball inflammation and eyeball congestion are reduced in Comparative Example 1 in which the fermentation process is not performed.

[0078] Furthermore, as shown in Comparative Example 3, even when an excessive amount of the ophthalmic drug raw material is used, the effects of improving eyeball inflammation and eyeball congestion are not significantly improved.

[0079] In addition, a cytotoxicity test of the eye drops prepared by Examples 1 to 3 and Comparative Examples 1 to 3 was performed, and the results are shown in Table 2 below.

[0080] Among them, for the cytotoxicity test, RAW 264.7 cells as a macrophage cell line were distributed from the Korean Cell Line Bank (KCLB) and cultured in an incubator adjusted to 37°C and 4% CO2 using RPMI 1640 medium containing 10% fetal bovine serum (FBS), 1% antibiotics and 2-mercaptoethanol (2-ME).

[0081] The cultured mouse macrophage cell line RAW2647 was incubated at 5×10 4 Cells / 100 μL were inoculated into a 96-well plate and then cultured in CO2 for 24 hours. After being treated with lipopolysaccharide (LPS) at a concentration of 1 μg / mL for 1 hour, the cells were treated with the eye drops of Examples 1 to 3 and Comparative Examples 1 to 3 and cultured in CO2 for 24 hours. Then, 100 μL of the culture supernatant was removed and treated with 10 μL of CCK-8 reagent respectively. After being cultured in CO2 for 3 hours, the absorbance was measured at 450 nm using an ELISA reader.

[0082]

[0083] distinguish Eye inflammation Control group 100.00±0.2 Lipopolysaccharide 97.64±0.9 Example 1 99.77±0.3 Example 2 99.80±0.4 Example 3 99.74±0.3 Comparative Example 1 98.90±0.5 Comparative Example 2 99.91±0.2 Comparative Example 3 97.58±0.6

[0084] As shown in Table 2, the Chinese herbal fermented eye drops prepared by Examples 1 to 3 of the present invention have very low cytotoxicity. On the contrary, the cytotoxicity of the eye drops prepared by Comparative Example 3 is relatively increased.

[0085] Therefore, the preparation method of the Chinese herbal fermented eye medicine of the present invention provides a Chinese herbal fermented eye medicine, which does not contain chemical components and has low cytotoxicity, is safe for the human body and exhibits excellent bactericidal effects, thereby showing the effect of improving eye diseases such as inflammation or congestion.

[0086] In addition, the Chinese herbal fermented eye drops of the present invention can improve dry eyes and regulate intraocular pressure and other eye diseases through the qi and blood circulation and blood-tonifying effects of Chinese medicine.

Claims

1. A method for preparing a Chinese herbal fermented eye drop, characterized in that: include: The Chinese medicinal material washing step comprises mixing the Chinese medicinal material mixture, vinegar and coarse salt in pure water and placing them therein, and then washing them; A heating step, putting the Chinese medicinal material mixture washed in the Chinese medicinal material washing step into a medicine decoction machine and mixing with purified water for heating; a cooling and fermentation step, wherein the decoction prepared in the heating step is cooled and then fermented; an ophthalmic drug raw material extraction step of extracting the ophthalmic drug raw material by distilling the fermented product prepared through the cooling and fermentation step; and an alkaline water mixing step, mixing alkaline water with the ophthalmic raw material prepared in the ophthalmic raw material extraction step, The Chinese medicinal material mixture is prepared by mixing equal amounts of wolfberry, rehmannia root, shiitake mushroom, ganoderma lucidum, mulberry leaf, mulberry root, deer antler, ginseng, astragalus, tangerine peel, chamomile, dandelion, tribulus terrestris, dodder seed, onion, purslane, millennium grass, ginger, jujube and licorice. The cooling and fermenting step comprises: cooling the decoction prepared in the heating step to a temperature of 40° C. to 45° C., then inoculating 0.01 to 0.1 parts by weight of the fermentation strain into 100 parts by weight of the cooled decoction, and fermenting for 14 to 16 hours, The fermentation strain is prepared by mixing Lactobacillus acidophilus, Lactobacillus bulgaricus, Lactobacillus paracasei, Bifidobacterium breve and Lactobacillus rhamnosus in a weight ratio of 1:1:1:1:

1. The alkaline water mixing step is to mix 0.05 to 0.15 parts by weight of the eye medicine raw material prepared in the eye medicine raw material extraction step with 100 parts by weight of alkaline water, The pH value of the alkaline water is 7.2 to 7.

6.

2. The method for preparing the Chinese herbal fermented eye drops according to claim 1, characterized in that: The Chinese medicinal material washing step comprises: 10 to 15 parts by weight of the Chinese medicinal material mixture, 1 to 2 parts by weight of vinegar and 0.2 to 0.4 parts by weight of crude salt are mixed in 100 parts by weight of pure water and left for 4 to 6 minutes, and then the Chinese medicinal materials are washed.

3. The method for preparing the Chinese herbal fermented eye drops according to claim 1, characterized in that: The heating step comprises: Relative to 100 parts by weight of the Chinese medicinal material mixture washed in the Chinese medicinal material washing step, 400 to 600 parts by weight of pure water are mixed, and heated at a temperature of 100°C to 120°C for 8 to 10 hours, and then heated at a temperature of 80°C to 90°C for 10 to 15 hours.

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