Microfluidic analysis device

Abstract

This application provides a microfluidic analysis device, including a driving device, a detection device, and a control device. The control device is used to: control the driving device to rotate a microfluidic disk so that a diluted sample flows into the colorimetric well of the microfluidic disk; control the driving device to rotate the microfluidic disk to mix the sample in the colorimetric well to obtain a detection solution; and acquire detection data of the detection solution from the detection device, and determine the detection result corresponding to the detection data. Specifically, between the flow of the diluted sample into the colorimetric well and the activation of the detection device to detect the detection solution, the driving device drives the microfluidic disk to rotate at a preset rotation parameter for at least a period of time to expel at least a portion of the gas in the colorimetric well. This prevents air bubbles in the colorimetric well from interfering with the accuracy of the detection data of the detection liquid, thereby improving the accuracy of the sample detection results.

Description

Technical Field

[0001] This application relates to the field of medical device technology, and more particularly to a microfluidic analysis device. Background Technology

[0002] Microfluidics is a system science and technology that precisely manipulates extremely small amounts of fluid (typically in the microliter, nanoliter, or picoliter range) within micrometer-scale channels. It serves as a crucial information acquisition and processing platform for modern biological and chemical sciences. This technology allows for the integration or near-integration of fundamental operations in biochemistry, such as sample preparation, reaction, detection, separation, or cell culture, sorting, and lysis, onto a single microchip. A network of microchannels enables control over the flow of fluid throughout the entire system.

[0003] Among them, detection chips used in microfluidics are typically disk-shaped structures, which can be called microfluidic disks. Microfluidic disks are designed with a number of colorimetric wells. The sample to be tested and the diluent are mixed during the rotation of the microfluidic disk and injected into the colorimetric wells through flow channels. The test result of the sample can be obtained by detecting the solution in the colorimetric wells. For example, reagents can be pre-encapsulated in the colorimetric wells. The mixed solution injected into the colorimetric wells reacts with the pre-encapsulated reagents to form a reactant solution to be tested. The test result of the sample can be obtained by detecting the reactant solution in the colorimetric wells.

[0004] The accuracy of the test results from microfluidic analysis devices using related technologies is sometimes insufficient. Summary of the Invention

[0005] This application provides a microfluidic analysis device designed to improve the accuracy of sample detection results.

[0006] In a first aspect, embodiments of this application provide a microfluidic analysis device, comprising:

[0007] A driving device is provided for driving a microfluidic disk to rotate. The microfluidic disk is provided with a sample chamber, a diluent chamber, a mixing chamber, and a colorimetric orifice. The mixing chamber is connected to the sample chamber and the diluent chamber through a first microchannel, and the mixing chamber is connected to the colorimetric orifice through a second microchannel.

[0008] A detection device is used to detect the detection solution in the colorimetric well on the microfluidic disk.

[0009] A control device, connected to the drive device and the detection device, is used for:

[0010] The driving device is controlled to drive the microfluidic disk to rotate, so that the sample in the sample chamber and the diluent in the diluent chamber flow into the mixing chamber through the first microchannel to mix and obtain a diluted sample, and the diluted sample in the mixing chamber flows into the colorimetric well through the second microchannel;

[0011] The driving device is controlled to drive the microfluidic disk to rotate, so as to mix the sample in the colorimetric well to obtain the detection solution; and the detection data of the detection device on the detection solution is acquired, and the detection result corresponding to the detection data is determined.

[0012] Specifically, between the inflow of the diluted sample into the colorimetric well and the activation of the detection device to detect the detection solution, the driving device is controlled to drive the microfluidic disk to rotate at a preset rotation parameter for at least a period of time to expel at least a portion of the gas in the colorimetric well. The preset rotation parameter includes at least a preset unit time rotation speed change, and the absolute value of the preset unit time rotation speed change is greater than the unit time rotation speed change of the microfluidic disk when the sample in the colorimetric well is mixed.

[0013] Secondly, embodiments of this application provide a microfluidic analysis device, comprising:

[0014] A driving device is used to drive the microfluidic disk to rotate, and the microfluidic disk is provided with a colorimetric orifice;

[0015] A detection device is used to detect samples in the colorimetric wells on the microfluidic disk.

[0016] A control device, connected to the drive device and the detection device, is used for:

[0017] The driving device is controlled to drive the microfluidic disk to rotate, so that the sample flows into the colorimetric well;

[0018] If it is determined that there are air bubbles in the colorimetric orifice, the driving device is controlled to drive the microfluidic disk to rotate with preset rotation parameters in order to expel at least part of the gas in the colorimetric orifice.

[0019] The detection device acquires the detection data of the sample in the colorimetric well, and acquires the detection result corresponding to the detection data.

[0020] The microfluidic analysis device provided in this application includes a driving device, a detection device, and a control device. The driving device drives a microfluidic disk to rotate. The microfluidic disk has a sample chamber, a diluent chamber, a mixing chamber, and a colorimetric orifice. The mixing chamber is connected to the sample chamber and the diluent chamber via a first microchannel, and to the colorimetric orifice via a second microchannel. The detection device detects the detection solution in the colorimetric orifice on the microfluidic disk. The control device controls the driving device to drive the microfluidic disk to rotate, so that the sample in the sample chamber and the diluent in the diluent chamber flow into the mixing chamber through the first microchannel to mix and obtain a diluted sample, and the diluted sample in the mixing chamber flows into the colorimetric orifice through the second microchannel. The control device controls the driving device to drive the microfluidic disk to rotate, so that the sample in the sample chamber and the diluent in the diluent chamber flow into the mixing chamber through the first microchannel to obtain a diluted sample, and the diluted sample in the mixing chamber flows into the colorimetric orifice through the second microchannel. The microfluidic disk rotates to mix the sample in the colorimetric well to obtain the detection solution; and the detection device acquires the detection data of the detection solution, determining the corresponding detection result. Specifically, between the inflow of the diluted sample into the colorimetric well and the activation of the detection device to detect the detection solution, the driving device drives the microfluidic disk to rotate at a preset rotation parameter for at least a certain period of time to expel at least some of the gas in the colorimetric well. The preset rotation parameter includes at least a preset unit time change in rotation speed, and the absolute value of the preset unit time change in rotation speed is greater than the unit time change in rotation speed of the microfluidic disk when mixing the sample in the colorimetric well. By driving the microfluidic disk to rotate at the preset rotation parameter for at least a certain period of time after the sample flows into the colorimetric well and before the detection device activates the detection solution, at least some of the gas in the colorimetric well can be expelled, preventing air bubbles in the colorimetric well from interfering with the accuracy of the detection data of the detection liquid, and improving the accuracy of the sample detection results.

[0021] It should be understood that the above general description and the following detailed description are exemplary and explanatory only, and do not limit the disclosure of the embodiments of this application. Attached Figure Description

[0022] To more clearly illustrate the technical solutions of the embodiments of this application, the drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are some embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0023] Figure 1 This is a schematic block diagram of a microfluidic analysis device provided in an embodiment of this application;

[0024] Figure 2 This is a schematic diagram of a microfluidic disk in one embodiment;

[0025] Figures 3a to 4c This is a schematic diagram of air bubbles inside the colorimetric orifice in one embodiment.

[0026] Explanation of reference numerals in the attached figures:

[0027] 110. Drive device; 120. Detection device; 130. Control device;

[0028] 10. Disc; 20. Sample chamber; 30. Diluent chamber; 40. Mixing chamber; 50. Colorimetric well; 61. First microchannel; 62. Second microchannel. Detailed Implementation

[0029] The technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this application, not all embodiments. Based on the embodiments of this application, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this application.

[0030] The flowchart shown in the attached diagram is for illustrative purposes only and does not necessarily include all content and operations / steps, nor does it necessarily have to be performed in the order described. For example, some operations / steps can be broken down, combined, or partially merged, so the actual execution order may change depending on the actual situation.

[0031] The following detailed description of some embodiments of this application is provided in conjunction with the accompanying drawings. Unless otherwise specified, the following embodiments and features can be combined with each other.

[0032] Please see Figure 1 This application provides a microfluidic analysis device. Exemplarily, the microfluidic analysis device can be a veterinary microfluidic analysis device, but it is not limited thereto; for example, it can also be a human microfluidic analysis device.

[0033] like Figure 1 As shown, the microfluidic analysis device includes a drive device 110, a detection device 120, and a control device 130.

[0034] The drive device 110 is used to drive the microfluidic disk to rotate, and transport the sample to the detection part of the microfluidic disk, such as the colorimetric well 50, for detection through centrifugal motion.

[0035] In some implementations, such as Figure 2 As shown, the microfluidic disk is provided with a sample chamber 20, a diluent chamber 30, a mixing chamber 40, and a colorimetric well 50. For example, the microfluidic disk includes a disk 10 and sample chamber 20, diluent chamber 30, mixing chamber 40, and colorimetric well 50 disposed on the disk 10.

[0036] The mixing chamber 40 is connected to the sample chamber 20 and the diluent chamber 30 through the first microchannel 61, and the mixing chamber 40 is connected to the colorimetric well 50 through the second microchannel 62.

[0037] The sample chamber 20 is used to hold the sample. For example, the disc is provided with a sample inlet hole that communicates with the sample chamber 20. The sample enters the sample chamber 20 through the sample inlet hole. Specifically, a syringe or other tool can be used to inject the sample into the sample chamber 20 through the sample inlet hole.

[0038] The diluent chamber 30 is used to contain the diluent, which can flow into the mixing chamber 40 to mix with the sample in the mixing chamber 40. Optionally, the diluent chamber 30 is used to quantify the diluent, with the quantified diluent entering the mixing chamber 40 under centrifugal force or gravity. Optionally, the disc is provided with a diluent filling hole communicating with the diluent chamber 30. During testing, the diluent can be manually added into the diluent filling hole. However, this is not limited to this; for example, the disc can be provided with a diluent encapsulation part communicating with the diluent chamber 30, and the diluent can also be pre-encapsulated in the diluent encapsulation part. During testing, it is only necessary to tear off the pre-encapsulated cap.

[0039] The mixing chamber 40 is connected to the sample chamber 20 and the diluent chamber 30 via corresponding first microchannels 61. When the microfluidic disk rotates, the sample in the sample chamber 20 flows into the mixing chamber 40 through the first microchannel 61, and the diluent in the diluent chamber 30 flows into the mixing chamber 40 through the first microchannel 61. The sample and diluent are mixed in the mixing chamber 40 to obtain a diluted sample. The mixing chamber 40 is connected to the colorimetric orifice 50 via a second microchannel 62. When the microfluidic disk rotates, the diluted sample in the mixing chamber 40 flows into the colorimetric orifice 50 through the second microchannel 62.

[0040] In some embodiments, the colorimetric wells 50 are pre-filled with a reagent, such as a lyophilized reagent, or may be a liquid; when the sample in the colorimetric well 50 is mixed, the reagent is mixed with the sample in the colorimetric well. The sample in the colorimetric well is mixed with the reagent to obtain the detection solution, and the detection result of the sample can be obtained based on the detection data of the detection device 120 on the detection solution. However, this is not a limitation; for example, the colorimetric wells 50 may not be pre-filled with a reagent. For instance, some colorimetric wells 50 on the microfluidic disk may be pre-filled with a reagent, while others are not. For the colorimetric wells 50 without pre-filled reagents, the detection device 120 can detect the sample in the colorimetric well, and the detection result of the sample can be determined based on the detection data of the detection device 120. The sample in the colorimetric well can also be referred to as the detection solution.

[0041] For example, the detection device 120 is used to detect the detection solution in the colorimetric well 50 on the microfluidic disk to obtain detection data. The control device 130 can process the detection data obtained by the detection device 120 to obtain the detection result of the sample. For example, the detection device 120 includes an optical detection device 120, which can acquire the absorbance of the sample in the colorimetric well 50. The control device 130 can determine the detection result of the sample based on the absorbance of the sample. Of course, it is not limited to this. For example, the detection device 120 can transmit the detection result corresponding to the detection data to the control device 130.

[0042] Optionally, the detection items of the microfluidic analysis device may include, but are not limited to, at least one of the following: biochemical detection, coagulation detection, immunoassay, and molecular detection. By using microfluidic discs to detect samples, it is possible to detect specific indicators using extremely small sample volumes.

[0043] By configuring the sample chamber 20, diluent chamber 30, mixing chamber 40, and colorimetric well 50, the sample can be diluted and mixed in the mixing chamber 40 and then enter the colorimetric well 50 for detection with a single sample addition. After adding the sample, the detection can be completed without manual operation, which is convenient, fast, and improves the accuracy of detection.

[0044] In some embodiments, the microfluidic disk may further include a sample separation chamber (not shown) for sample separation. The sample separation chamber includes a first separation chamber and a second separation chamber connected to the first separation chamber. The first separation chamber is connected to the sample chamber 20 and the mixing chamber 40, and the sample flowing out of the sample chamber 20 is separated within the sample separation chamber. In some use cases, such as when the sample is whole blood, blood separation is required through the sample separation chamber to separate serum and blood cells for testing. Of course, the sample is not limited to whole blood samples.

[0045] In some embodiments, the microfluidic analysis device may include a reaction chamber for accommodating a microfluidic disc and a sample inlet channel for delivering the microfluidic disc into the reaction chamber, so that the user can place the microfluidic disc in the sample inlet channel so that the sample inlet channel delivers the microfluidic disc into the reaction chamber, and a drive device 110 is used to drive the microfluidic disc in the reaction chamber to rotate.

[0046] In this embodiment, the control device 130 is connected to the drive device 110 and the detection device 120. The control device 130 is used to: control the drive device 110 to drive the microfluidic disk to rotate, so that the sample in the sample chamber 20 and the diluent in the diluent chamber 30 flow into the mixing chamber 40 through the first microchannel 61 to mix and obtain a diluted sample, and the diluted sample in the mixing chamber 40 flows into the colorimetric well 50 through the second microchannel 62; control the drive device 110 to drive the microfluidic disk to rotate, so that the sample in the colorimetric well and the reagent are mixed to obtain a detection solution; and acquire the detection data of the detection device 120 on the detection solution, and determine the detection result corresponding to the detection data.

[0047] Between the inflow of the diluted sample into the colorimetric well 50 and the activation of the detection device to detect the detection solution, the control drive device 110 drives the microfluidic disk to rotate at a preset rotation parameter for at least a period of time to expel at least a portion of the gas in the colorimetric well 50.

[0048] For example, the control drive device 110 drives the microfluidic disk to rotate at a preset rotation parameter for at least a period of time, including controlling the control drive device 110 to drive the microfluidic disk to rotate at least one revolution at the preset rotation parameter. Of course, it is not limited to this. For example, the microfluidic disk can be driven to rotate at least half a revolution or at least a quarter revolution at the preset rotation parameter.

[0049] Air is present in the colorimetric well 50 of the microfluidic disk. When diluted sample flows into the colorimetric well 50, the air will exist in the colorimetric well 50 in the form of bubbles. The presence of bubbles in the colorimetric well 50 affects the accuracy of the detection results.

[0050] like Figure 3a or Figure 4a As shown, the colorimetric orifice 50 contains air bubbles. For example, the air bubbles have different optical properties from the liquid being detected in the colorimetric orifice 50. The presence of air bubbles will interfere with the optical detection device 120 in acquiring detection data such as the absorbance of the liquid being detected, resulting in a decrease in the accuracy of the detection data and results.

[0051] In this embodiment, by driving the microfluidic disk to rotate at a preset rotation parameter for at least a period of time after the sample flows into the colorimetric orifice 50 and before the detection device starts detecting the detection solution, at least part of the gas in the colorimetric orifice 50 can be discharged, preventing air bubbles in the colorimetric orifice 50 from interfering with the accuracy of the detection data of the detection liquid and improving the accuracy of the sample detection results.

[0052] The preset rotation parameters include at least a preset change in rotational speed per unit time. For example, the change in rotational speed per unit time is the change in rotational speed per second (s), where the rotational speed can be revolutions per minute (rpm), and the unit of the change in rotational speed per second can be expressed as rpm / s; the change in rotational speed per unit time can also be angular acceleration, and the unit of angular acceleration can be expressed as rad / s. 2 (radians per second squared)

[0053] It should be noted that the preset unit time rotation speed change can be a positive number, i.e., greater than 0, indicating that the microfluidic disk rotates faster; and / or the preset unit time rotation speed change can be a negative number, i.e., less than 0, indicating that the microfluidic disk rotates slower.

[0054] Specifically, the absolute value of the preset unit-time rotational speed change is greater than the unit-time rotational speed change of the microfluidic disk when the sample from the contrast color aperture is mixed; for example, the angular acceleration of the microfluidic disk when it is driven to rotate with preset rotation parameters is greater than the angular acceleration of the microfluidic disk when the sample from the contrast color aperture is mixed. For instance, the unit-time rotational speed change of the microfluidic disk when the sample from the contrast color aperture is mixed is 3000 rpm / s, and the preset unit-time rotational speed change can be selected from 5000-15000 rpm / s; however, it is not limited to this.

[0055] By controlling the drive device 110 to drive the microfluidic disk to rotate at a large angular acceleration, such as rapid acceleration or deceleration, at least part of the gas in the colorimetric orifice 50 can be discharged based on the difference in fluid motion characteristics between the gas and the liquid.

[0056] The bubbles generated within the colorimetric orifice 50 can be categorized into suspended bubbles and adherent bubbles. For example... Figure 3a As shown, within the dashed circular frame, there are air bubbles in contact with the inner wall of the colorimetric orifice 50; these air bubbles can be called adhering air bubbles. Figure 4a As shown, bubbles are suspended in the liquid within the dashed circular frame of the colorimetric orifice 50. These bubbles can be called suspended bubbles.

[0057] For example, with a temperature of 37 degrees Celsius (°C) at orifice 50, the preset unit time change in rotational speed of the microfluidic disk during acceleration or deceleration is 6000 rpm / s (corresponding to an angular acceleration of 200π rad / s²). 2 For example, the Euler force generated by acceleration and / or deceleration acts on the liquid and air bubbles in the colorimetric orifice 50 in the tangential direction of the angular velocity. The Euler force f acting on the liquid in the colorimetric orifice 50 is... Euler1 The Euler force f acting on the bubbles in the colorimetric orifice 50 Euler2 It can be represented as follows:

[0058]

[0059]

[0060] Where, ρ 液 ρ represents the density of the liquid in orifice 50. 气 The density of the bubbles in the colorimetric orifice 50 is represented by r, and the radius of the colorimetric orifice 50 is represented by r.

[0061] Please see Figure 3a and Figure 3b When the microfluidic disk is driven to accelerate or decelerate rapidly, the difference in the Euler force acting on the attached bubble and the liquid can cause the attached bubble to move relative to the colorimetric orifice 50 and detach from the wall of the colorimetric orifice 50.

[0062] Please see Figures 4a to 4b When the microfluidic disk is driven to accelerate or decelerate rapidly, due to the difference in centrifugal force between the liquid and the bubbles, the bubbles that detach from the wall of the colorimetric orifice 50 and the suspended bubbles are discharged from the microchannel connected to the colorimetric orifice 50 under centrifugal action.

[0063] In some embodiments, when the driving device 110 drives the microfluidic disk to rotate at the preset rotation parameters, the absolute value of the change in rotational speed per unit time of the microfluidic disk is greater than or equal to a preset change threshold. This allows for rapid acceleration or deceleration of the microfluidic disk's rotation, enabling the discharge of at least a portion of the gas from the colorimetric orifice 50 based on the differences in fluid motion characteristics between the gas and liquid.

[0064] For example, the change threshold is greater than the change in rotational speed per unit time of the microfluidic disk when mixing the liquid in the mixing chamber 40 or the colorimetric orifice 50. Typically, to ensure a longer service life of the microfluidic analyzer, the rotational speed and the change in rotational speed per unit time of the microfluidic disk are limited to prevent the drive device 110 (such as its motor) from overheating and accelerating aging when driving the microfluidic disk to rotate rapidly, accelerate quickly, or decelerate rapidly. In this embodiment, the microfluidic disk can be driven to rotate with a larger change in rotational speed per unit time to expel at least a portion of the gas from the colorimetric orifice 50, thereby improving the accuracy of detection.

[0065] For example, the absolute value of the preset unit time speed change is selected from 5000-15000 rpm / s. For example, the preset unit time speed change can be any of the following: 5000 rpm / s, 5500 rpm / s, 8000 rpm / s, 10000 rpm / s, 11000 rpm / s, 12000 rpm / s, 15000 rpm / s.

[0066] In some embodiments, when the driving device 110 drives the microfluidic disk to rotate at the preset rotation parameters, in a first time period, the change in rotational speed per unit time of the microfluidic disk is greater than or equal to the change threshold; and in a second time period, the change in rotational speed per unit time of the microfluidic disk is negative and its absolute value is greater than or equal to the change threshold. Driving the microfluidic disk to accelerate rapidly in the first time period and then decelerate it in the second time period can improve the efficiency of bubble removal. For example, the Euler forces generated by rapid acceleration and deceleration act in different directions on the liquid and bubbles. By changing the direction of the Euler force, the adhering bubbles can be impacted, resulting in more efficient and thorough removal of the adhering bubbles, allowing them to be discharged from the microchannel under centrifugal force.

[0067] Optionally, when the driving device 110 drives the microfluidic disk to rotate at the preset rotation parameters, the change in rotational speed per unit time of the microfluidic disk is greater than or equal to the change threshold, and the change in rotational speed per unit time of the microfluidic disk is negative and its absolute value is greater than or equal to the change threshold, alternating multiple times. For example, the operation in the first time period and the operation in the second time period are alternating multiple times, such as performing at least two cycles of rapid acceleration to rapid deceleration, i.e., rapid acceleration-rapid deceleration-rapid acceleration-rapid deceleration. Through multiple rapid accelerations and decelerations, multiple impacts are applied to the wall-adhering bubbles, which can remove the wall-adhering bubbles more efficiently and thoroughly, so that the bubbles can be discharged from the microchannel under centrifugal force.

[0068] In some embodiments, the preset rotation parameters may further include a preset rotation speed. The preset rotation speed is greater than the rotation speed of the microfluidic disk when the sample in the colorimetric orifice 50 is being mixed. For example, at least at a moment after rapid acceleration, the rotation speed of the microfluidic disk is greater than the rotation speed of the microfluidic disk when the sample in the colorimetric orifice 50 is being mixed. By increasing the rotation speed of the microfluidic disk, the centrifugal effect can be enhanced, and air bubbles in the colorimetric orifice 50 can be removed more thoroughly.

[0069] In some embodiments, before the sample in the colorimetric well is mixed, for example before the sample in the colorimetric well is mixed with the reagent, the control drive device 110 drives the microfluidic disk to rotate at a preset rotation parameter for at least a period of time to expel at least a portion of the gas in the colorimetric well 50.

[0070] Before the sample flows into the colorimetric well 50, air is already present in the well. This air exists as bubbles as the sample flows into the well, and the presence of these bubbles affects the accuracy of the detection results. By purging at least some of the gas from the well 50 before the sample and reagent are mixed, it is possible to prevent the bubbles from breaking down into more bubbles during the mixing process, thus preventing interference with the detection. Furthermore, purging at least some of the gas from the well 50 before mixing ensures a larger contact area between the sample and reagent, which is beneficial for uniform mixing.

[0071] In some embodiments, after the sample in the colorimetric well is mixed and before the detection device starts detecting the detection solution, for example after the sample in the colorimetric well is mixed with the reagent and before the detection device starts detecting the detection solution, the control drive device 110 drives the microfluidic disk to rotate at a preset rotation parameter for at least a period of time to expel at least a portion of the gas in the colorimetric well 50.

[0072] During the mixing of the sample and reagent, the reagent reacts with the sample, sometimes generating bubbles. The presence of these bubbles can affect the accuracy of the detection results. By purging at least some of the gas from the colorimetric well 50 after the sample and reagent have been mixed and before the detection device is activated to detect the test solution, interference from bubbles in the colorimetric well 50 can be prevented.

[0073] Optionally, before the sample in the colorimetric well is mixed with the reagent, the driving device 110 can be controlled to drive the microfluidic disk to rotate at a preset rotation parameter for at least a period of time to expel at least a portion of the gas in the colorimetric well 50; and after the sample in the colorimetric well is mixed with the reagent and before the detection device starts detecting the detection solution, the driving device 110 can be controlled to drive the microfluidic disk to rotate at a preset rotation parameter for at least a period of time to expel at least a portion of the gas in the colorimetric well 50.

[0074] For example, the sample detection process of the microfluidic analysis device may include: the microfluidic disc rotating to allow the sample and diluent to flow into the mixing chamber 40; the microfluidic disc rotating to mix the sample and diluent flowing into the mixing chamber 40; the microfluidic disc rotating to allow the diluted sample in the mixing chamber 40 to flow into the colorimetric well 50; the control drive device 110 driving the microfluidic disc to rotate at a preset rotation parameter to expel at least part of the gas in the colorimetric well 50; the microfluidic disc rotating to mix the reagent and sample in the colorimetric well 50; the control drive device 110 driving the microfluidic disc to rotate at a preset rotation parameter to expel at least part of the gas in the colorimetric well 50; obtaining the detection data of the detection solution by the detection device 120; and obtaining the detection result corresponding to the detection data.

[0075] By venting at least some of the gas in the colorimetric well 50 before the sample is mixed with the reagent in the colorimetric well 50, it is possible to prevent the bubbles from decomposing into more bubbles during the mixing process of the sample and the reagent. Thus, after the sample and the reagent are mixed, the microfluidic disk can be driven to rotate at a preset rotation parameter by controlling the drive device 110, which can more thoroughly vent the bubbles in the colorimetric well 50.

[0076] In some embodiments, when the sample in the colorimetric well is being mixed, the control drive device 110 drives the microfluidic disk to rotate at a preset rotation parameter for at least a period of time to expel at least a portion of the gas in the colorimetric well 50; that is, at least a portion of the gas can also be expelled when the sample in the colorimetric well 50 is being mixed. For example, during the period when the control drive device 110 drives the microfluidic disk to rotate at the preset rotation parameter, the absolute value of the angular acceleration of the microfluidic disk is greater than the angular acceleration of the microfluidic disk during other times when the sample in the colorimetric well is being mixed with the reagent.

[0077] During the mixing process of the sample and reagent, the reagent and sample dissolve and react, sometimes producing bubbles. By venting at least some of the gas in the colorimetric well 50 during the mixing process, it is possible to prevent the bubbles from decomposing into more bubbles during continued mixing. This also ensures a large contact area between the sample and reagent, which is beneficial for uniform mixing. By controlling the drive device 110 to drive the microfluidic disk to rotate at a preset rotation parameter for at least a period of time during the mixing process of the sample and reagent, the bubble removal efficiency of the sample can be improved. For example, after the sample is mixed, it is not necessary to control the drive device 110 to drive the microfluidic disk to rotate at the preset rotation parameter; alternatively, the drive device 110 can be controlled to drive the microfluidic disk to rotate at the preset rotation parameter after the sample is mixed, in order to more thoroughly remove the bubbles in the colorimetric well 50.

[0078] In some embodiments, the control device 130 is also used to acquire an image of the colorimetric aperture 50; for example, the detection device 120 includes a camera or a separate camera is provided outside the detection device 120 to acquire an image of the colorimetric aperture 50.

[0079] For example, the control drive device 110 drives the microfluidic disk to rotate at a preset rotation parameter for at least a period of time to expel at least a portion of the gas in the colorimetric orifice 50, including: when it is determined from the image of the colorimetric orifice 50 that there are air bubbles in the colorimetric orifice 50, the control drive device 110 drives the microfluidic disk to rotate at a preset rotation parameter for at least a period of time to expel at least a portion of the gas in the colorimetric orifice 50.

[0080] For example, before and / or after mixing the sample in the colorimetric well 50, the presence of air bubbles in the well 50 is determined based on the image of the well 50. If air bubbles are found in the well 50, the drive device 110 is controlled to drive the microfluidic disk to rotate at preset rotation parameters for at least a certain period of time to expel at least some of the gas from the well 50. If there are no air bubbles or only a small number of air bubbles in the well 50, the microfluidic disk may not be driven to rotate at the preset rotation parameters to improve detection efficiency.

[0081] In some embodiments, the control drive device 110 drives the microfluidic disk to rotate at a preset rotation parameter for at least a period of time to expel at least a portion of the gas in the colorimetric orifice 50, including: when the current detection mode is the debubbling mode, the control drive device 110 drives the microfluidic disk to rotate at a preset rotation parameter for at least a period of time to expel at least a portion of the gas in the colorimetric orifice 50.

[0082] For example, the current detection mode can be determined based on user settings. For instance, the controller can control the display to show a settings interface that includes settings controls for the debubbling mode. When the user opens the debubbling mode settings controls, the current detection mode is determined to be debubbling mode.

[0083] When the current detection mode is the degassing mode, between the time the diluted sample flows into the colorimetric well 50 and the time the detection device starts detecting the detection solution, the control drive device 110 drives the microfluidic disk to rotate at a preset rotation parameter for at least a period of time to expel at least a portion of the gas in the colorimetric well 50.

[0084] For example, when the current detection mode is the de-bubbling mode, before and / or after mixing the sample in the colorimetric well, it is determined whether there are air bubbles in the colorimetric well 50 based on the image of the colorimetric well 50, and when it is determined that there are air bubbles in the colorimetric well 50, the control drive device 110 drives the microfluidic disk to rotate at a preset rotation parameter for at least a period of time to expel at least part of the gas in the colorimetric well 50.

[0085] The microfluidic analysis device provided in this application includes a driving device 110, a detection device 120, and a control device 130. The driving device 110 drives a microfluidic disk to rotate. The microfluidic disk is provided with a sample chamber 20, a diluent chamber 30, a mixing chamber 40, and a colorimetric orifice 50. The mixing chamber 40 is connected to the sample chamber 20 and the diluent chamber 30 through a first microchannel 61, and to the colorimetric orifice 50 through a second microchannel 62. The detection device 120 is used to detect the detection solution in the colorimetric orifice 50 on the microfluidic disk. The control device 130 is used to: control the driving device 110 to drive the microfluidic disk to rotate, so that the sample in the sample chamber 20 and the diluent in the diluent chamber 30 flow into the mixing chamber 40 through the first microchannel 61 for mixing to obtain a diluted sample, and the diluted sample in the mixing chamber 40 flows into the mixing chamber 40 through the second microchannel 62. A sample 62 flows into the colorimetric well 50; a control drive device 110 drives the microfluidic disk to rotate, mixing the sample in the colorimetric well to obtain a detection solution; and the detection device 120 acquires the detection data of the detection solution, determining the corresponding detection result; wherein, between the dilute sample flowing into the colorimetric well 50 and the detection device starting to detect the detection solution, the control drive device 110 drives the microfluidic disk to rotate at a preset rotation parameter for at least a period of time to expel at least some of the gas in the colorimetric well 50. The preset rotation parameter includes at least a preset unit time rotation speed change, and the absolute value of the preset unit time rotation speed change is greater than the unit time rotation speed change of the microfluidic disk when mixing the sample in the colorimetric well. By driving the microfluidic disk to rotate at the preset rotation parameter for at least a period of time after the sample flows into the colorimetric well 50 and before the detection device starts to detect the detection solution, at least some of the gas in the colorimetric well 50 can be expelled, preventing air bubbles in the colorimetric well 50 from interfering with the accuracy of the detection data of the detection liquid, and improving the accuracy of the sample detection result.

[0086] Please refer to the foregoing embodiments. Figure 1 In another embodiment of this application, the microfluidic analysis device includes a driving device 110, a detection device 120, and a control device 130.

[0087] The driving device 110 is used to drive the microfluidic disk to rotate, and the microfluidic disk is provided with colorimetric orifices 50. In this embodiment, the structure of the microfluidic disk is not specifically limited. For example, when the microfluidic disk rotates, the sample in the sample cavity 20 of the microfluidic disk can flow directly into part of the colorimetric orifices 50 through the microchannel without dilution.

[0088] The detection device 120 is used to detect the detection solution in the colorimetric well 50 on the microfluidic disk. This application embodiment does not specifically limit the detection principle and structure of the detection device 120.

[0089] The control device 130 is connected to the drive device 110 and the detection device 120. The control device 130 is used to: control the drive device 110 to drive the microfluidic disk to rotate so that the sample flows into the colorimetric well 50; if it is determined that there are air bubbles in the colorimetric well 50, control the drive device 110 to drive the microfluidic disk to rotate at a preset rotation parameter so as to expel at least part of the gas in the colorimetric well 50; acquire the detection data of the sample in the colorimetric well 50 by the detection device 120, and acquire the detection result corresponding to the detection data.

[0090] In some embodiments, the control device 130 is further configured to: acquire an image of the colorimetric orifice 50; and determine whether there are air bubbles in the colorimetric orifice 50 based on the image. When it is determined that there are air bubbles in the colorimetric orifice 50, the control drive device 110 drives the microfluidic disk to rotate at a preset rotation parameter for at least a period of time to expel at least a portion of the gas from the colorimetric orifice 50. If there are no air bubbles or only a small number of air bubbles in the colorimetric orifice 50, the microfluidic disk may not be driven to rotate at the preset rotation parameter to improve detection efficiency.

[0091] The specific principles and implementation methods of the control method of the microfluidic analysis device provided in this application embodiment are similar to those of the microfluidic analysis device in the aforementioned embodiment, and will not be repeated here.

[0092] It should be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to limit the scope of the application.

[0093] It should also be understood that the term “and / or” as used in this application and the appended claims means any combination of one or more of the associated listed items and all possible combinations, and includes such combinations.

[0094] The above description is merely a specific embodiment of this application, but the scope of protection of this application is not limited thereto. Any person skilled in the art can easily conceive of various equivalent modifications or substitutions within the technical scope disclosed in this application, and these modifications or substitutions should all be covered within the scope of protection of this application. Therefore, the scope of protection of this application should be determined by the scope of the claims.

Claims

1. A microfluidic analysis device, characterized in that, include: A driving device is provided for driving a microfluidic disk to rotate. The microfluidic disk is provided with a sample chamber, a diluent chamber, a mixing chamber, and a colorimetric orifice. The mixing chamber is connected to the sample chamber and the diluent chamber through a first microchannel, and the mixing chamber is connected to the colorimetric orifice through a second microchannel. A detection device is used to detect the detection solution in the colorimetric well on the microfluidic disk. A control device, connected to the drive device and the detection device, is used for: The driving device is controlled to drive the microfluidic disk to rotate, so that the sample in the sample chamber and the diluent in the diluent chamber flow into the mixing chamber through the first microchannel to mix and obtain a diluted sample, and the diluted sample in the mixing chamber flows into the colorimetric well through the second microchannel; The driving device is controlled to drive the microfluidic disk to rotate, so as to mix the sample in the colorimetric well to obtain the detection solution; and the detection data of the detection device on the detection solution is acquired, and the detection result corresponding to the detection data is determined. Specifically, between the inflow of the diluted sample into the colorimetric well and the activation of the detection device to detect the detection solution, the driving device is controlled to drive the microfluidic disk to rotate at a preset rotation parameter for at least a period of time to expel at least a portion of the gas in the colorimetric well. The preset rotation parameter includes at least a preset unit time rotation speed change, and the absolute value of the preset unit time rotation speed change is greater than the unit time rotation speed change of the microfluidic disk when the sample in the colorimetric well is mixed.

2. The microfluidic analysis device according to claim 1, characterized in that, The colorimetric wells are pre-filled with reagents, and when the samples in the colorimetric wells are mixed, the reagents are mixed with the samples in the colorimetric wells.

3. The microfluidic analysis device according to claim 1, characterized in that, Before mixing the sample in the colorimetric well, the driving device is controlled to drive the microfluidic disk to rotate at a preset rotation parameter for at least a period of time to expel at least a portion of the gas in the colorimetric well. and / or After the sample in the colorimetric well is mixed and before the detection device starts detecting the detection solution, the driving device is controlled to drive the microfluidic disk to rotate at a preset rotation parameter for at least a period of time to expel at least a portion of the gas in the colorimetric well. and / or When mixing the sample in the colorimetric well, the driving device is controlled to drive the microfluidic disk to rotate at a preset rotation parameter for at least a period of time to expel at least a portion of the gas in the colorimetric well.

4. The microfluidic analysis device according to claim 1, characterized in that, When the driving device drives the microfluidic disk to rotate at the preset rotation parameters, the absolute value of the change in rotation speed per unit time of the microfluidic disk is greater than or equal to the preset change threshold.

5. The microfluidic analysis device according to claim 4, characterized in that, When the driving device drives the microfluidic disk to rotate at the preset rotation parameters, in the first time period, the change in rotation speed of the microfluidic disk per unit time is greater than or equal to the change threshold. And in the second time period, the change in rotational speed of the microfluidic disk per unit time is negative and the absolute value is greater than or equal to the change threshold.

6. The microfluidic analysis device according to claim 5, characterized in that, When the driving device drives the microfluidic disk to rotate with the preset rotation parameters, the change in rotational speed of the microfluidic disk per unit time is greater than or equal to the change threshold, and the change in rotational speed of the microfluidic disk per unit time is negative and the absolute value is greater than or equal to the change threshold, alternating multiple times.

7. The microfluidic analysis device according to claim 6, characterized in that, The threshold value of the change is greater than the change in rotational speed of the microfluidic disk per unit time when the liquid in the mixing chamber or the colorimetric orifice is mixed.

8. The microfluidic analysis device according to any one of claims 1-7, characterized in that, The absolute value of the preset unit time change in rotational speed is selected from 5000-15000 rpm / s.

9. The microfluidic analysis device according to any one of claims 1-7, characterized in that, The preset rotation parameters also include a preset rotation speed, which is greater than the rotation speed of the microfluidic disk when mixing the sample in the colorimetric orifice.

10. The microfluidic analysis device according to any one of claims 1-7, characterized in that, The control device is also used to acquire an image of the colorimetric aperture; The control of the driving device to drive the microfluidic disk to rotate at a preset rotation parameter for at least a period of time to expel at least a portion of the gas in the colorimetric orifice includes: When it is determined from the image of the colorimetric orifice that there are air bubbles in the colorimetric orifice, the driving device is controlled to drive the microfluidic disk to rotate at a preset rotation parameter for at least a period of time in order to expel at least a portion of the gas in the colorimetric orifice.

11. The microfluidic analysis device according to any one of claims 1-7, characterized in that, The control of the driving device to drive the microfluidic disk to rotate at a preset rotation parameter for at least a period of time to expel at least a portion of the gas in the colorimetric orifice includes: When the current detection mode is the debubbling mode, the driving device is controlled to drive the microfluidic disk to rotate at a preset rotation parameter for at least a period of time in order to expel at least part of the gas in the colorimetric orifice.

12. A microfluidic analysis device, characterized in that, include: A driving device is used to drive a microfluidic disk to rotate, and the microfluidic disk is provided with a colorimetric orifice; The detection device is used to detect the sample in the colorimetric well on the microfluidic disk. A control device, connected to the drive device and the detection device, is used for: The driving device is controlled to drive the microfluidic disk to rotate, so that the sample flows into the colorimetric well; If it is determined that there are air bubbles in the colorimetric orifice, the driving device is controlled to drive the microfluidic disk to rotate with preset rotation parameters in order to expel at least part of the gas in the colorimetric orifice. The detection device acquires the detection data of the sample in the colorimetric well, and acquires the detection result corresponding to the detection data; The preset rotation parameters include at least a preset unit time rotation speed change, and the absolute value of the preset unit time rotation speed change is greater than the unit time rotation speed change of the microfluidic disk when the sample in the colorimetric orifice is mixed.

13. The microfluidic analysis device according to claim 12, characterized in that, The control device is also used for: Acquire an image of the colorimetric aperture; Determine whether there are air bubbles in the colorimetric orifice based on the image of the colorimetric orifice.

Images