A medical sodium hyaluronate gel dressing and its preparation method

Abstract

The present invention relates to a medical sodium hyaluronate gel dressing and a preparation method thereof, belonging to the technical field of biomaterials. The specific components of the medical sodium hyaluronate gel dressing disclosed in the present invention are sodium hyaluronate, sodium chloride, carbomer and purified water. The preparation method of sodium hyaluronate is as follows: (1) Put sodium hyaluronate solid into an oxidation solvent for mixing, heat up and keep warm to obtain solution a; (2) Mix sodium hyaluronate solid with a buffer solution, then add a degrading enzyme, and obtain solution b after degradation; (3) Make sodium hyaluronate solid into a solution, ultrasonically mix it with solution a and solution b, then filter and separate, and obtain sodium hyaluronate after drying. Through the oxidation degradation and enzymatic hydrolysis process of sodium hyaluronate and then mixing with the raw material solution, the present invention has sodium hyaluronate with different molecular weights from low to high, and it has the effect of promoting soothing and moisturizing simultaneously from inside and outside the skin.

Description

Technical Field

[0001] The invention belongs to the technical field of biomaterials and relates to a medical sodium hyaluronate gel dressing and a preparation method thereof. Background Art

[0002] Sodium hyaluronate (HA) is a high-molecular mucopolysaccharide that is widely present in human skin and other tissues and has a good moisturizing effect. The repair patch made from sodium hyaluronate is suitable for skin care after minimally invasive surgery such as facial laser and photorejuvenation. It can reduce pigmentation, lighten scars, and promote the repair of skin damage.

[0003] HA is mostly produced and used in the form of sodium hyaluronate. The efficacy of different types of sodium hyaluronate varies greatly. For example, high molecular weight sodium hyaluronate (molecular weight>500,000) has a good mucosal protective effect, while low molecular weight sodium hyaluronate (molecular weight 10,000-500,000) and oligomeric sodium hyaluronate (molecular weight <10,000) have good advantages in skin penetration and oral absorption. At present, hyaluronic acid products are mainly concentrated in hyaluronic acid with a single molecular weight distribution, with a narrow molecular weight distribution width and uneven quality. Summary of the invention

[0004] The present invention relates to a medical sodium hyaluronate gel dressing and a preparation method thereof, and belongs to the technical field of biomaterials. The specific components of the medical sodium hyaluronate gel dressing disclosed in the present invention are sodium hyaluronate, sodium chloride, carbomer and purified water, wherein the preparation method of sodium hyaluronate is as follows: (1) sodium hyaluronate solid is put into an oxidizing solvent for mixing, heating and keeping warm to obtain solution a; (2) sodium hyaluronate solid is mixed with a buffer solution, and then a degrading enzyme is added to obtain solution b after degradation; (3) sodium hyaluronate solid is made into a solution, ultrasonically mixed with solution a and solution b, and then filtered and separated, and dried to obtain sodium hyaluronate. The present invention oxidizes and degrades sodium hyaluronate and enzymolysis process, and then mixes it with a raw material solution. Sodium hyaluronate has different molecular weights from low to high, which simultaneously promotes the effects of soothing and hydrating from the inside and outside of the skin.

[0005] The purpose of the present invention can be achieved through the following technical solutions:

[0006] A medical sodium hyaluronate gel dressing, which is composed of the following raw materials in parts by weight: 0.05-0.5 parts of sodium hyaluronate, 0.01-0.1 parts of sodium chloride, 0.5-5 parts of carbomer, and 95-100 parts of purified water. The preparation method of the sodium hyaluronate comprises the following steps:

[0007] (1) placing sodium hyaluronate solid into an oxidizing solvent, mixing, heating and maintaining the temperature to obtain solution a;

[0008] (2) Mix the sodium hyaluronate solid with the buffer solution, then add the degrading enzyme, and obtain solution b after degradation;

[0009] (3) Prepare a sodium hyaluronate solution from the sodium hyaluronate solid and deionized water, ultrasonically mix it with solution a and solution b, and then concentrate through a nanofilm and dry to obtain sodium hyaluronate.

[0010] Further, the molecular weight of the sodium hyaluronate solid is 1 million - 2 million, the solid - liquid ratio of the sodium hyaluronate solid to the oxidation solvent in step (1) is 0.1 - 0.2 g:1 mL, the oxidation solvent is a hydrogen peroxide solution, the mass concentration of the hydrogen peroxide solution is 2 - 3%, and the temperature and time for heat preservation are 70 - 75 °C and 24 - 30 h respectively.

[0011] Further, the solid - liquid ratio of the sodium hyaluronate solid to the buffer solution in step (2) is 80 - 90 g:1 L, the mass ratio of the sodium hyaluronate solid to the degrading enzyme is 100:0.01 - 0.026, the degrading enzyme is composed of β - glucuronidase and β - N - acetylglucosaminidase with a mass ratio of 1:1, and the time and temperature for degradation are 1 - 2 h and 35 - 37 °C respectively.

[0012] Further, the preparation method of the buffer solution in step (2) is: Dissolve disodium hydrogen phosphate dihydrate, sodium hydrogen phosphate dodecahydrate and sodium chloride in deionized water, and stir at room temperature until completely dissolved to obtain the buffer solution.

[0013] Further, the solid - liquid ratio of the total mass of disodium hydrogen phosphate dihydrate, sodium hydrogen phosphate dodecahydrate and sodium chloride to deionized water is 9 - 10 g:1 L, and the mass ratio among disodium hydrogen phosphate dihydrate, sodium hydrogen phosphate dodecahydrate and sodium chloride is 0.045:0.5 - 0.6:8.5 - 9.

[0014] Further, the mass concentration of the sodium hyaluronate solution in step (3) is 0.5 - 1.5%, and the volume ratio of the sodium hyaluronate solution, solution a and solution b is 5 - 10:2:2.

[0015] Further, the time and frequency for ultrasonic treatment in step (3) are 30 - 45 min and 40 - 60 kHz respectively, the temperature and time for drying are 50 - 60 °C and 1 - 2 h respectively, and the pore size of the nanofilm is 0.1 - 0.4 nm.

[0016] Further, the preparation method of the sodium hyaluronate gel dressing is as follows: Add sodium hyaluronate and carbomer into purified water, stir and disperse, heat up to 85°C ± 5°C while stirring, and keep stirring for 20 minutes after complete dissolution. Then, cool down to 40°C ± 5°C while stirring, add sodium chloride, and stir for another 10 minutes to complete.

[0017] Advantages of the present invention:

[0018] 1. In the present invention, sodium hyaluronate is degraded by oxidative degradation and enzymatic hydrolysis processes respectively to reduce its molecular weight to achieve the degradation purpose. Among them, β-glucuronidase and β-N-acetylglucosaminidase in the enzymatic hydrolysis process act together to degrade sodium hyaluronate to obtain a smaller molecular weight, and then it is mixed with the raw material solution, so that sodium hyaluronate with different molecular weight grades from low to high exists in the sodium hyaluronate. Therefore, in the sodium hyaluronate gel dressing prepared by the present invention, the small molecular weight enters the skin, while the large molecular weight sodium hyaluronate forms a protective film on the skin surface, promoting the effects of soothing and moisturizing simultaneously from the inside and outside. Specific embodiments

[0019] To further elaborate on the technical means and effects adopted by the present invention to achieve the predetermined invention purpose, the following examples are used to describe in detail the specific embodiments, structures, features and their effects of the present invention as follows.

[0020] Example 1

[0021] A medical sodium hyaluronate gel dressing, which is composed of the following raw materials in parts by weight: 0.05 part of sodium hyaluronate, 0.01 part of sodium chloride, 0.5 part of carbomer, and 95 parts of purified water. The preparation method of the sodium hyaluronate includes the following steps:

[0022] (1) Put the sodium hyaluronate solid into an oxidation solvent and mix, heat up and keep warm to obtain solution a;

[0023] (2) Take the sodium hyaluronate solid and mix it with a buffer solution, then add a degrading enzyme, and obtain solution b after degradation;

[0024] (3) Make a sodium hyaluronate solution from the sodium hyaluronate solid and deionized water, ultrasonically mix it with solution a and solution b, and then concentrate it through a nano-film and dry it to obtain sodium hyaluronate.

[0025] The molecular weight of the sodium hyaluronate solid is 1,000,000. In step (1), the solid-liquid ratio of the sodium hyaluronate solid to the oxidation solvent is 0.1 g:1 mL, where the oxidation solvent is a hydrogen peroxide solution, the mass concentration of the hydrogen peroxide solution is 2%, and the temperature and time for keeping warm are 70°C and 30 h respectively.

[0026] In step (2), the solid-liquid ratio of sodium hyaluronate solid to buffer solution is 80 g:1 L, and the mass ratio of sodium hyaluronate solid to degradation enzyme is 100:0.01. The degradation enzyme consists of β-glucuronidase and β-N-acetylglucosaminidase with a mass ratio of 1:1. The degradation time and temperature are 1 h and 35 °C respectively.

[0027] The preparation method of the buffer solution in step (2) is as follows: Take disodium hydrogen phosphate dihydrate, disodium hydrogen phosphate dodecahydrate and sodium chloride, dissolve them in deionized water, and stir at room temperature until completely dissolved to obtain the buffer solution.

[0028] The solid-liquid ratio of the total mass of disodium hydrogen phosphate dihydrate, disodium hydrogen phosphate dodecahydrate and sodium chloride to deionized water is 9 g:1 L, and the mass ratio among disodium hydrogen phosphate dihydrate, disodium hydrogen phosphate dodecahydrate and sodium chloride is 0.045:0.5:8.5.

[0029] In step (3), the mass concentration of the sodium hyaluronate solution is 0.5%, and the volume ratio of the sodium hyaluronate solution, solution a and solution b is 5:2:2.

[0030] In step (3), the time and frequency of ultrasonic treatment are 30 min and 40 kHz respectively, the temperature and time of drying are 50 °C and 1 h respectively, and the pore size of the nanofilm is 0.1 nm.

[0031] The preparation method of the sodium hyaluronate gel dressing is as follows: Add sodium hyaluronate and carbomer into purified water and stir to disperse. While stirring, heat up to 70 °C. After complete dissolution, keep stirring at a constant temperature for 20 minutes, then stir and cool down to 35 °C, add sodium chloride, and stir for another 10 minutes to complete.

[0032] The purchase source of the carbomer: Jiufenglong - 9007 - 20 - 9.

[0033] Example 2

[0034] A medical sodium hyaluronate gel dressing, which is composed of the following raw materials in parts by weight: 0.25 part of sodium hyaluronate, 0.05 part of sodium chloride, 0.25 part of carbomer, and 97.5 parts of purified water. The preparation method of the sodium hyaluronate includes the following steps:

[0035] (1) Put the sodium hyaluronate solid into an oxidation solvent for mixing, heat up and keep warm to obtain solution a;

[0036] (2) Take the sodium hyaluronate solid and mix it with the buffer solution, then add the degradation enzyme, and obtain solution b after degradation;

[0037] (3) The sodium hyaluronate solid is made into a sodium hyaluronate solution with deionized water, ultrasonically mixed with solution a and solution b, and then concentrated through a nanofilm, and sodium hyaluronate is obtained after drying.

[0038] The molecular weight of the sodium hyaluronate solid is 1.5 million. In the step (1), the solid-liquid ratio of the sodium hyaluronate solid to the oxidation solvent is 0.15 g:1 mL, where the oxidation solvent is a hydrogen peroxide solution, the mass concentration of the hydrogen peroxide solution is 2.5%, and the temperature and time for heat preservation are 72.5 °C and 27 h respectively.

[0039] In the step (2), the solid-liquid ratio of the sodium hyaluronate solid to the buffer solution is 85 g:1 L, and the mass ratio of the sodium hyaluronate solid to the degrading enzyme is 100:0.02. The degrading enzyme is composed of β-glucuronidase and β-N-acetylglucosaminidase with a mass ratio of 1:1. The time and temperature for degradation are 1.5 h and 36 °C respectively.

[0040] The preparation method of the buffer solution in the step (2) is as follows: Take disodium hydrogen phosphate dihydrate, disodium hydrogen phosphate dodecahydrate and sodium chloride, dissolve them in deionized water, and stir at room temperature until completely dissolved to obtain the buffer solution.

[0041] The solid-liquid ratio of the total mass of disodium hydrogen phosphate dihydrate, disodium hydrogen phosphate dodecahydrate and sodium chloride to deionized water is 9.5 g:1 L, and the mass ratio among disodium hydrogen phosphate dihydrate, disodium hydrogen phosphate dodecahydrate and sodium chloride is 0.045:0.55:8.7.

[0042] In the step (3), the mass concentration of the sodium hyaluronate solution is 1%, and the volume ratio of the sodium hyaluronate solution, solution a and solution b is 7.5:2:2.

[0043] In the step (3), the time and frequency of ultrasonic treatment are 40 min and 50 kHz respectively, the temperature and time for drying are 55 °C and 1.5 h respectively, and the pore size of the nanofilm is 0.2 nm.

[0044] The preparation method of the sodium hyaluronate gel dressing is as follows: Add sodium hyaluronate and carbomer into purified water and stir to disperse. While stirring, heat up to 85 °C. After complete dissolution, keep stirring and heat-preserving for 20 minutes, then stir and cool down to 40 °C, add sodium chloride, and stir for another 10 minutes to complete.

[0045] The purchase source of the carbomer: Jiufenglong - 9007 - 20 - 9.

[0046] Example 3

[0047] A medical sodium hyaluronate gel dressing, the medical sodium hyaluronate gel dressing is composed of the following raw materials in parts by weight: 0.5 part of sodium hyaluronate, 0.1 part of sodium chloride, 5 parts of carbomer, and 100 parts of purified water. The preparation method of the sodium hyaluronate includes the following steps:

[0048] (1) Put the sodium hyaluronate solid into an oxidation solvent and mix, heat up and keep warm to obtain solution a;

[0049] (2) Take the sodium hyaluronate solid and mix it with a buffer solution, then add a degrading enzyme, and after degradation, obtain solution b;

[0050] (3) The sodium hyaluronate solid and deionized water are made into a sodium hyaluronate solution, ultrasonically mixed with solution a and solution b, and then concentrated through a nanofilm and dried to obtain sodium hyaluronate.

[0051] The molecular weight of the sodium hyaluronate solid is 2 million. In step (1), the solid-liquid ratio of the sodium hyaluronate solid to the oxidation solvent is 0.2 g:1 mL, where the oxidation solvent is a hydrogen peroxide solution, the mass concentration of the hydrogen peroxide solution is 3%, and the temperature and time for heat preservation are 75 °C and 230 h respectively.

[0052] In step (2), the solid-liquid ratio of the sodium hyaluronate solid to the buffer solution is 90 g:1 L, the mass ratio of the sodium hyaluronate solid to the degrading enzyme is 100:0.026, where the degrading enzyme is composed of β-glucuronidase and β-N-acetylglucosaminidase with a mass ratio of 1:1, and the time and temperature for degradation are 2 h and 37 °C respectively.

[0053] The preparation method of the buffer solution in step (2) is: Take disodium hydrogen phosphate dihydrate, disodium hydrogen phosphate dodecahydrate and sodium chloride and dissolve them in deionized water, and stir at room temperature until completely dissolved to obtain the buffer solution.

[0054] The solid-liquid ratio of the total mass of disodium hydrogen phosphate dihydrate, disodium hydrogen phosphate dodecahydrate and sodium chloride to deionized water is 10 g:1 L, where the mass ratio among disodium hydrogen phosphate dihydrate, disodium hydrogen phosphate dodecahydrate and sodium chloride is 0.045:0.6:9.

[0055] In step (3), the mass concentration of the sodium hyaluronate solution is 1.5%, and the volume ratio of the sodium hyaluronate solution, solution a and solution b is 10:2:2.

[0056] In step (3), the time and frequency of ultrasonic treatment are 45 min and 60 kHz respectively, the temperature and time for drying are 60 °C and 2 h respectively, and the pore size of the nanofilm is 0.4 nm.

[0057] The preparation method of the sodium hyaluronate gel dressing is as follows: Add sodium hyaluronate and carbomer into purified water and stir to disperse. While stirring, heat up to 90 °C. After all are dissolved, keep stirring at a constant temperature for 20 minutes, then stir and cool down to 45 °C, add sodium chloride, and stir for another 10 minutes to complete.

[0058] The purchase source of the carbomer: Jiufenglong - 9007 - 20 - 9.

[0059] Comparative Example 1

[0060] Based on Example 3, a medical sodium hyaluronate gel dressing, which is composed of the following raw materials in parts by weight: 0.5 part of sodium hyaluronate, 0.1 part of sodium chloride, 5 parts of carbomer, and 100 parts of purified water. The preparation method of the sodium hyaluronate includes the following steps:

[0061] (1) Take solid sodium hyaluronate and mix it with a buffer solution, then add a degrading enzyme, and after degradation, obtain solution b;

[0062] (2) Make solid sodium hyaluronate into a sodium hyaluronate solution with deionized water, ultrasonically mix it with solution b, and then concentrate it through a nanofilm and dry it to obtain sodium hyaluronate.

[0063] The solid - liquid ratio of the solid sodium hyaluronate to the buffer solution is 90 g:1 L, the mass ratio of the solid sodium hyaluronate to the degrading enzyme is 100:0.026, wherein the degrading enzyme is composed of β - glucuronidase and β - N - acetylglucosaminidase with a mass ratio of 1:1, and the time and temperature of the degradation are 2 h and 37 °C respectively.

[0064] The preparation method of the buffer solution in step (1) is: Take disodium hydrogen phosphate dihydrate, sodium hydrogen phosphate dodecahydrate, and sodium chloride and dissolve them in deionized water, and stir at room temperature until all are dissolved to obtain the buffer solution.

[0065] The solid - liquid ratio of the total mass of disodium hydrogen phosphate dihydrate, sodium hydrogen phosphate dodecahydrate, and sodium chloride to deionized water is 10 g:1 L, and the mass ratio among disodium hydrogen phosphate dihydrate, sodium hydrogen phosphate dodecahydrate, and sodium chloride is 0.045:0.6:9.

[0066] In step (2), the mass concentration of the sodium hyaluronate solution is 1.5%, and the volume ratio of the sodium hyaluronate solution to solution b is 10:2.

[0067] In step (2), the time and frequency of ultrasonic treatment are 45 min and 60 kHz respectively, the temperature and time of drying are 60 °C and 2 h respectively, and the pore size of the nanofilm is 0.4 nm.

[0068] The preparation method of the sodium hyaluronate gel dressing is as follows: Add sodium hyaluronate and carbomer into purified water and stir to disperse. While stirring, heat up to 90 °C. After all are dissolved, keep stirring and heating for 20 minutes, then stir and cool down to 45 °C, add sodium chloride, and stir for another 10 minutes to complete.

[0069] The purchase source of the carbomer: Jiufenglong - 9007 - 20 - 9.

[0070] Comparative Example 2

[0071] On the basis of Example 3, a medical sodium hyaluronate gel dressing, the medical sodium hyaluronate gel dressing is composed of the following raw materials in parts by weight: 0.5 part of sodium hyaluronate, 0.1 part of sodium chloride, 5 parts of carbomer, and 100 parts of purified water. The preparation method of the sodium hyaluronate includes the following steps:

[0072] (1) Put the sodium hyaluronate solid into an oxidation solvent and mix, heat up and keep warm to obtain solution a;

[0073] (2) Make a sodium hyaluronate solution from the sodium hyaluronate solid and deionized water, ultrasonically mix it with solution a, and then concentrate it through a nano - membrane, and dry it to obtain sodium hyaluronate.

[0074] The molecular weight of the sodium hyaluronate solid is 2 million. In step (1), the solid - liquid ratio of the sodium hyaluronate solid to the oxidation solvent is 0.2 g:1 mL, where the oxidation solvent is a hydrogen peroxide solution, the mass concentration of the hydrogen peroxide solution is 3%, and the temperature and time for keeping warm are 75 °C and 230 h respectively.

[0075] In step (2), the mass concentration of the sodium hyaluronate solution is 1.5%, and the volume ratio of the sodium hyaluronate solution to solution a is 10:4.

[0076] In step (2), the time and frequency of ultrasonic treatment are 45 min and 60 kHz respectively, the temperature and time for drying are 60 °C and 2 h respectively, and the pore size of the nano - membrane is 0.4 nm.

[0077] The preparation method of the sodium hyaluronate gel dressing is as follows: Add sodium hyaluronate and carbomer into purified water and stir to disperse. While stirring, heat up to 90 °C. After all are dissolved, keep stirring and heating for 20 minutes, then stir and cool down to 45 °C, add sodium chloride, and stir for another 10 minutes to complete.

[0078] The purchase source of the carbomer: Jiufenglong - 9007 - 20 - 9.

[0079] Comparative Example 3

[0080] On the basis of Example 3, remove the β-glucuronidase in step (2) during the preparation of sodium hyaluronate, and replace it with an equal mass of β-N-acetylglucosaminidase, with other conditions being the same as in Example 3.

[0081] Comparative Example 4

[0082] On the basis of Example 3, remove the β-N-acetylglucosaminidase in step (2) during the preparation of sodium hyaluronate, and replace it with an equal mass of β-glucuronidase, with other conditions being the same as in Example 3.

[0083] 1. Performance Test

[0084] Molecular weight detection: According to QB / T 4416-2012 "Sodium Hyaluronate for Cosmetic Raw Materials", the molecular weights of the sodium hyaluronate prepared in Example 3 and Comparative Examples 1-4 were detected, and the detection results are shown in Table 1;

[0085] Moisture retention test: Use the medical sodium hyaluronate gel dressings prepared in Example 3 and Comparative Examples 1-4 as samples. Select 45 effective volunteer subjects, aged 20-55 years old, and no products can be used on the test site for the previous 2-3 days. Before the test, the subjects uniformly cleaned the inner sides of their forearms, marked the 4×4 cm test area, evenly coated the samples on the left and right arms, with a dosage of 3 mg / cm 2 , and use a skin moisture tester to measure the water content of the test skin area before applying the product and at 30 min, 1 h, 3 h, and 6 h after application. Each area was measured in parallel 5 times, and the average value was used as the tester. The tests of the same subject were completed by the same measuring personnel. All testers were in an indoor space with constant temperature and humidity before and after the test. The moisture retention test formula is: Skin moisture increase (%) = (water content after application - water content before application) / water content before application × 100%, and the test results are shown in Table 2.

[0086] Table 1 Molecular Weight Detection Results

[0087] Specimen 0.2 ten thousand - 10 thousand 10 thousand - 300 thousand 300 thousand - 600 thousand ≥ 1 million Example 3 26.5％ 24.8％ 22.6％ 26.1％ Comparative Example 1 38.9％ 10.17％ 2.13％ 48.8％ Comparative Example 2 5.79％ 9.84％ 42.27％ 42.1％ Comparative Example 3 13.8％ 12.4％ 35.7％ 38.1％ Comparative Example 4 12.9％ 13.％ 36.1％ 38％

[0088] As can be seen from Table 1, the molecular weight grades of Example 3 are very uniform, while in Comparative Examples 1-4, the proportion of the molecular weight grades is either too high or too low; in Comparative Example 1, the sodium hyaluronate solid was not degraded with an oxidation solvent, and the proportion of its molecular weight grades in the range of 10,000-300,000 and 300,000-600,000 decreased significantly; in Comparative Example 2, the sodium hyaluronate solid was not degraded with a degrading enzyme, and the molecular weight grades in the range of 0.2×10,000-10,000 and 10,000-300,000 decreased; in Comparative Examples 3-4, only β-glucuronidase or β-N-acetylglucosaminidase was added as the degrading enzyme, and the proportion of the molecular weight classification in the range of 0.2×10,000-10,000 and 10,000-300,000 decreased.

[0089] Table 2 Moisture Retention Test Results

[0090]

[0091] It can be analyzed from Table 2 that the moisture retention effect of Example 3 is better than that of Comparative Examples 1-4, and the moisture retention effect of Comparative Example 2 is the worst. In Comparative Example 1, since the proportion of molecular weight grades of 10,000-300,000 and 300,000-600,000 is relatively small, when the moisture retention effect of sodium hyaluronate with a large molecular weight fails on the skin surface, less moisture is absorbed under the skin surface, so the moisture retention decreases; in Comparative Example 2, the proportion of sodium hyaluronate with a small molecular weight is the least, and the deep layer of the skin cannot be supplemented with moisture. Therefore, after the moisture on the skin surface and the lower layer is lost, the moisture retention effect drops significantly at 3 h; in Comparative Examples 3-4, the proportion of molecular weight grades of 0.2 million-10,000 and 10,000-300,000 is relatively small compared with that of molecular weight grades of 300,000-600,000 and ≥1 million, but the difference is not as large as that of Comparative Example 2, so the decrease in moisture gradually decreases.

[0092] The above are only the preferred embodiments of the present invention, and do not impose any formal limitations on the present invention. Although the present invention has been disclosed as above with preferred embodiments, it is not intended to limit the present invention. Any person skilled in the art can make some changes or modifications to equivalent embodiments by using the disclosed technical content within the scope of the technical solution of the present invention. However, as long as it does not depart from the content of the technical solution of the present invention, any simple modification, equivalent change and modification made to the above embodiments according to the technical essence of the present invention still fall within the scope of the technical solution of the present invention.

Claims

1. A medical sodium hyaluronate gel dressing, characterized in that: The medical sodium hyaluronate gel dressing is composed of the following raw materials in parts by weight: 0.05-0.5 parts of sodium hyaluronate, 0.01-0.1 parts of sodium chloride, 0.5-5 parts of carbomer, and 95-100 parts of purified water. The preparation method of the sodium hyaluronate comprises the following steps: (1) placing sodium hyaluronate solid into an oxidizing solvent, mixing, heating and maintaining the temperature to obtain solution a; (2) mixing sodium hyaluronate solid with a buffer solution, and then adding a degrading enzyme to obtain a solution b after degradation; wherein the degrading enzyme is composed of β-glucuronidase and β-N-acetylglucosaminidase in a mass ratio of 1:1; (3) Sodium hyaluronate solid is mixed with deionized water to form a sodium hyaluronate solution, which is ultrasonically mixed with solution a and solution b, and then concentrated by a nano-membrane and dried to obtain sodium hyaluronate.

2. A medical sodium hyaluronate gel dressing according to claim 1, characterized in that: The molecular weight of the sodium hyaluronate solid is 1-2 million, the solid-liquid ratio of the sodium hyaluronate solid to the oxidizing solvent in the step (1) is 0.1-0.2 g:1 mL, wherein the oxidizing solvent is a hydrogen peroxide solution, the mass concentration of the hydrogen peroxide solution is 2-3%, and the insulation temperature and time are 70-75° C. and 24-30 h, respectively.

3. A medical sodium hyaluronate gel dressing according to claim 1, characterized in that: In the step (2), the solid-liquid ratio of sodium hyaluronate solid to buffer solution is 80-90 g:1 L, the mass ratio of sodium hyaluronate solid to degrading enzyme is 100:0.01-0.026, and the degradation time and temperature are 1-2 h and 35-37 ° C, respectively.

4. The medical sodium hyaluronate gel dressing according to claim 1, characterized in that: The preparation method of the buffer solution in step (2) is as follows: disodium hydrogen phosphate dihydrate, disodium hydrogen phosphate dodecahydrate and sodium chloride are dissolved in deionized water, and stirred at room temperature until all are dissolved to obtain a buffer solution.

5. The medical sodium hyaluronate gel dressing according to claim 4, characterized in that: The solid-liquid ratio of the total mass of the disodium hydrogen phosphate dihydrate, disodium hydrogen phosphate dodecahydrate and sodium chloride to deionized water is 9-10g:1L, wherein the mass ratio of the disodium hydrogen phosphate dihydrate, disodium hydrogen phosphate dodecahydrate and sodium chloride is 0.045:0.5-0.6:8.5-9.

6. The medical sodium hyaluronate gel dressing according to claim 1, characterized in that: The mass concentration of the sodium hyaluronate solution in step (3) is 0.5-1.5%, and the volume ratio of the sodium hyaluronate solution, solution a and solution b is 5-10:2:

2.

7. The medical sodium hyaluronate gel dressing according to claim 1, characterized in that: In the step (3), the ultrasonic time and frequency are 30-45 min and 40-60 kHz respectively, the drying temperature and time are 50-60° C. and 1-2 h respectively, and the pore size of the nanomembrane is 0.1-0.4 nm.

8. A method for preparing the medical sodium hyaluronate gel dressing according to any one of claims 1 to 7, characterized in that: The preparation method of the sodium hyaluronate gel dressing is as follows: sodium hyaluronate and carbomer are added to purified water and stirred to disperse, and the temperature is raised to 85°C±5°C while stirring. After all the hyaluronate and carbomer are dissolved, the temperature is kept stirring for 20 minutes, and then the temperature is cooled to 40°C±5°C while stirring, and sodium chloride is added, and the dressing is stirred for another 10 minutes.

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