Organic fertilizer containing fish protein peptide and preparation method thereof

Abstract

The present invention relates to an organic fertilizer containing fish protein peptides and a preparation method thereof, and belongs to the technical field of organic fertilizers. The organic fertilizer containing fish protein peptides comprises the following components: nitrogen, phosphorus and potassium fertilizers, fermentation products, antibacterial agents, medium and trace elements and fish protein polypeptides; the preparation of the antibacterial agent comprises: mixing chitosan oligosaccharides and acid solution and then ultrasonically treating to obtain a mixed solution; in a nitrogen atmosphere, oleic acid, cerium ammonium nitrate solution and anhydrous ethanol are mixed and stirred evenly, slowly added to the mixed solution for heating treatment, acetone is added for precipitation, solids are filtered out, immersed in a copper chloride solution, stirred and mixed, and then allowed to stand, and the solids are placed in an oven for drying to obtain the antibacterial agent. The present invention introduces oleic acid into the chitosan oligosaccharide molecular chain, and then copper chloride metal salts are adsorbed on its surface to form a more compact structure compound, thereby enhancing hydrophobicity and improving its antifungal activity.

Description

Technical Field

[0001] The invention belongs to the technical field of organic fertilizers and relates to an organic fertilizer containing fish protein peptide and a preparation method thereof. Background Art

[0002] Fish protein peptide is a special protein that comes from the by-products of fish processing. This protein is not only rich in nitrogen, but also plays a very important role in organic fertilizers and is a very high-quality ingredient. However, due to the high protein characteristics of fish protein peptides, if the humidity of the environment is high or the temperature is suitable during storage, mold growth may occur. The growth of this mold will cause the organic fertilizer to mildew. This mildew will not only affect the fertilizer efficiency and reduce its use effect, but may also produce some substances that are harmful to crops, thereby affecting the growth of crops. Therefore, in the process of storing fish protein peptides, special attention should be paid to controlling the humidity and temperature of the environment to prevent the growth of mold and ensure the quality and effect of the fertilizer.

[0003] As a natural polysaccharide, the application of chitosan oligosaccharide in antifungal has received wide attention. However, chitosan oligosaccharide has also exposed some defects in practical applications, especially in terms of its hydrophobicity and stability. First, from the perspective of hydrophobicity, chitosan oligosaccharide contains more hydrophilic groups in its molecular structure, which makes it difficult to effectively penetrate the mold cell wall when it comes into contact with mold cells, thus affecting its antifungal effect. The mold cell wall is highly hydrophobic, and the hydrophilicity of chitosan oligosaccharide makes it difficult to bind tightly to the mold cell wall, resulting in its antifungal activity not being fully exerted. Secondly, under different environmental conditions, such as temperature, pH value, the presence of enzymes, etc., the structure of chitosan oligosaccharide may change, thereby affecting its antifungal performance. Under high temperature or extreme pH conditions, chitosan oligosaccharide may degrade or change its structure, resulting in reduced antifungal activity. Summary of the invention

[0004] The object of the present invention is to provide an organic fertilizer containing fish protein peptide and a preparation method thereof. The present invention introduces oleic acid into the chitosan oligosaccharide molecular chain, and then adsorbs copper chloride metal salt on the surface thereof to form a compound with a denser structure, thereby enhancing hydrophobicity and thus improving its antifungal activity.

[0005] The purpose of the present invention can be achieved through the following technical solutions:

[0006] An organic fertilizer containing fish protein peptides comprises the following components by weight:

[0007] 18-30 parts of nitrogen, phosphorus and potassium fertilizers, 90-110 parts of fermentation products, 5-8 parts of antibacterial agents, 2-5 parts of trace elements and 20-40 parts of fish protein polypeptides.

[0008] As a preferred technical solution of the present invention, the nitrogen, phosphorus and potassium fertilizer is prepared by mixing ammonium nitrate, urea and potassium dihydrogen phosphate in a weight ratio of 2-3:1-1.5:1.5-2.0.

[0009] As a preferred technical solution of the present invention, the trace elements are calcium, magnesium, sulfur, silicon, iron, boron, manganese and zinc, and the above elements are in equal amounts.

[0010] The invention discloses a method for preparing the antibacterial agent, comprising the following steps:

[0011] 1) stirring and mixing the chitosan oligosaccharide and the acid solution, and then performing ultrasonic treatment to obtain a mixed solution;

[0012] 2) Under a nitrogen atmosphere, oleic acid, ammonium cerium nitrate solution and anhydrous ethanol are mixed and stirred evenly, and then slowly added to the mixed solution for heating treatment, acetone is added for precipitation, and then the solid is filtered out and immersed in a cupric chloride solution for stirring and mixing, and then allowed to stand, and the solid is placed in an oven for drying to obtain an antibacterial agent.

[0013] As a preferred technical solution of the present invention, in step 1), the ultrasonic treatment is performed at a frequency of 30-40 KHz and a power of 300-350 W for 15-20 min.

[0014] As a preferred technical solution of the present invention, in step 1), the usage ratio of the chitosan oligosaccharide and the acid solution is 5.0-5.2 g:180 mL; the acid solution is prepared by mixing citric acid and deionized water in a mass ratio of 1 g:30 mL.

[0015] As a preferred technical solution of the present invention, in step 2), the usage ratio of oleic acid, ammonium cerium nitrate solution, anhydrous ethanol, mixed solution and cupric chloride solution is 3.2-3.6g: 0.60-0.62g: 20-25mL: 350-360g: 40-45g; the concentration of ammonium cerium nitrate solution is 0.02g / mL.

[0016] As a preferred technical solution of the present invention, in step 2), the cupric chloride solution is prepared by mixing cupric chloride and deionized water in a mass ratio of 2-3.5:30-35; the heating treatment is stirring at 35-45°C for 4-5h; the washing is washing 3 times with anhydrous ethanol; the stirring and mixing time is 45-60min; the standing time is 3-5h; and the drying is drying at 40-45°C to constant weight.

[0017] The present invention discloses a method for preparing the fermentation product, comprising the following steps:

[0018] 1) Weigh straw powder and deionized water, stir and mix for 45-60 minutes, then add humic acid and mix evenly to obtain base material;

[0019] 2) After the fermentation bacteria agent and deionized water are evenly mixed, the mixture is sprayed on the base material, fermented for 7-14 days, and dried until the water content is less than 15wt% to obtain a fermentation product.

[0020] As a preferred technical solution of the present invention, the straw powder is obtained by crushing, grinding and drying the straw, and the straw is one or more of rice, wheat, corn, soybean and sorghum straw.

[0021] As a preferred technical solution of the present invention, the mass ratio of the straw powder, deionized water and humic acid is 20-23:20-25:50.

[0022] As a preferred technical solution of the present invention, the mass ratio of the base material, the fermentation agent and the deionized water is 100:1.0-1.2:15-16; the fermentation agent is a mixed agent of yeast, Streptomyces griseus and actinomycetes, and the amount of live yeast in the fermentation agent is 1.2×10 8 cfu / mL, the number of live Streptomyces griseus was 1×10 8 cfu / mL, the amount of live actinomycetes is 1×10 8 cfu / mL.

[0023] The invention discloses a method for preparing the organic fertilizer containing fish protein peptide as described above, and the method comprises the following steps: uniformly mixing nitrogen, phosphorus and potassium fertilizers, fermentation products, antibacterial agents, trace elements and fish protein peptide, and granulating to obtain the organic fertilizer.

[0024] As a preferred technical solution of the present invention, the preparation method of the fish protein polypeptide comprises the following steps: taking 50g of tilapia minced meat, adding 50mL of 0.1mol / L hydrochloric acid solution to homogenize, then adding 200mL of 0.1mol / L sodium chloride solution, after 300W ultrasonic treatment for 5min, centrifuging at 4000r / min at 4°C, taking the precipitate, adding it to an acid mixture consisting of 0.5mol / L hydrochloric acid and 0.2mol / L citric acid in a mass ratio of 1:5, extracting at room temperature for 1h, then centrifuging at 3000r / min at 4°C for 10min, filtering the filtrate, and filtering The residue was extracted three times, all the filtrates were combined, acetone was added, and the mixture was allowed to stand for 30 minutes, followed by centrifugation at 5000 r / min for 10 minutes. The precipitate was vacuum freeze-dried for 24 hours, and the obtained particles were added at 1 g:10 mL into a phosphate buffer solution with a pH value of 7.0 to obtain a mixed solution, to which 0.1% neutral protease was added according to the mixed solution, and the mixture was ultrasonically treated at 300 W for 3 minutes, and then the enzyme was inactivated in boiling water for 10 minutes. After cooling to room temperature, acetone was added and allowed to stand for 30 minutes, and the mixture was centrifuged at 6000 r / min for 20 minutes. After vacuum freeze-drying, fish protein polypeptides were obtained.

[0025] Beneficial effects of the present invention:

[0026] The invention introduces more hydrophobic oleic acid molecules into the chitosan oligosaccharide molecular chain by means of chemical modification to form a chitosan oligosaccharide-oleic acid compound, and then adsorbs copper chloride metal salt with strong antibacterial property on the surface of the compound by coordination with copper ions to form a compound with a denser structure, thereby enhancing the hydrophobicity of the chitosan oligosaccharide molecules and making it easier for the chitosan oligosaccharide molecules to interact with the hydrophobic components on the mold cell wall, thereby improving the antifungal activity thereof, and the introduction of copper ions enhances the stability of the chitosan oligosaccharide, protects the chitosan oligosaccharide molecules from being affected by external environmental factors such as pH value changes, temperature fluctuations, etc., thereby maintaining the integrity of the structure and ensuring that the chitosan oligosaccharide can maintain a good antifungal effect under various environmental conditions. DETAILED DESCRIPTION

[0027] In order to further illustrate the technical means and effects adopted by the present invention to achieve the predetermined invention purpose, the specific implementation methods, structures, features and effects of the present invention are described in detail below in combination with the embodiments.

[0028] Example 1

[0029] An organic fertilizer containing fish protein peptides comprises the following components by weight:

[0030] 18 parts of nitrogen, phosphorus and potassium fertilizers, 90 parts of fermentation products, 5 parts of antibacterial agents, 2 parts of trace elements and 20 parts of fish protein peptides;

[0031] The nitrogen, phosphorus and potassium fertilizer is prepared by mixing ammonium nitrate, urea and potassium dihydrogen phosphate in a weight ratio of 2:1:1.5;

[0032] The trace elements are calcium, magnesium, sulfur, silicon, iron, boron, manganese and zinc, and each of the above elements is in equal amounts;

[0033] The preparation method of the antibacterial agent comprises the following steps:

[0034] 1) stirring and mixing the chitosan oligosaccharide and the acid solution, and then performing ultrasonic treatment to obtain a mixed solution;

[0035] 2) Under a nitrogen atmosphere, oleic acid, ammonium cerium nitrate solution and anhydrous ethanol are mixed and stirred evenly, and then slowly added to the mixture for heating treatment, acetone is added for precipitation, and then the solid is filtered out, immersed in a cupric chloride solution, stirred and mixed, and allowed to stand, and the solid is placed in an oven for drying to obtain an antibacterial agent;

[0036] In step 1), the ultrasonic treatment is performed at a frequency of 30 KHz and a power of 300 W for 15 min; the amount ratio of the chitosan oligosaccharide to the acid solution is 5.0 g:180 mL; the acid solution is prepared by mixing citric acid and deionized water at a mass ratio of 1 g:30 mL;

[0037] In step 2), the dosage ratio of oleic acid, ammonium cerium nitrate solution, anhydrous ethanol, mixed solution and cupric chloride solution is 3.2g:0.60g:20mL:350g:40g; the concentration of the ammonium cerium nitrate solution is 0.02g / mL, and the solvent is deionized water; the cupric chloride solution is prepared by mixing cupric chloride and deionized water in a mass ratio of 2:30; the heating treatment is stirring at 35°C for 4h; the washing is washing 3 times with anhydrous ethanol; the stirring and mixing time is 45min; the standing time is 3h; and the drying is drying at 40°C to constant weight.

[0038] The method for preparing the fermentation product comprises the following steps:

[0039] 1) Weigh straw powder and deionized water, stir and mix for 45 minutes, then add humic acid and mix evenly to obtain a base material; the straw powder is obtained by crushing, grinding and drying straw, and the straw is rice; the mass ratio of the straw powder, deionized water and humic acid is 20:20:50;

[0040] 2) After the fermentation agent and deionized water are evenly mixed, the mixture is sprayed on the base material, fermented for 7 days, and dried until the water content is less than 15wt% to obtain a fermentation product; the mass ratio of the base material, the fermentation agent and the deionized water is 100:1.0:15; the fermentation agent is a mixed agent of yeast, Streptomyces griseus and actinomycetes, and the amount of live yeast in the fermentation agent is 1.2×10 8cfu / mL, the number of live Streptomyces griseus was 1×10 8 cfu / mL, the amount of live actinomycetes is 1×10 8 cfu / mL.

[0041] A method for preparing an organic fertilizer containing fish protein peptide comprises the following steps: uniformly mixing nitrogen, phosphorus and potassium fertilizers, fermentation products, antibacterial agents, medium and trace elements and fish protein peptide, and granulating to obtain the organic fertilizer.

[0042] Example 2

[0043] An organic fertilizer containing fish protein peptides comprises the following components by weight:

[0044] 22 parts of nitrogen, phosphorus and potassium fertilizers, 97 parts of fermentation products, 6 parts of antibacterial agents, 3 parts of trace elements and 27 parts of fish protein peptides;

[0045] The nitrogen, phosphorus and potassium fertilizer is prepared by mixing ammonium nitrate, urea and potassium dihydrogen phosphate in a weight ratio of 2.3:1.2:1.7;

[0046] The trace elements are calcium, magnesium, sulfur, silicon, iron, boron, manganese and zinc, and each of the above elements is in equal amounts;

[0047] The preparation method of the antibacterial agent comprises the following steps:

[0048] 1) stirring and mixing the chitosan oligosaccharide and the acid solution, and then performing ultrasonic treatment to obtain a mixed solution;

[0049] 2) Under a nitrogen atmosphere, oleic acid, ammonium cerium nitrate solution and anhydrous ethanol are mixed and stirred evenly, and then slowly added to the mixture for heating treatment, acetone is added for precipitation, and then the solid is filtered out, immersed in a cupric chloride solution, stirred and mixed, and allowed to stand, and the solid is placed in an oven for drying to obtain an antibacterial agent;

[0050] In step 1), the ultrasonic treatment is performed at a frequency of 33KHz and a power of 315W for 17 minutes; the ratio of the chitosan oligosaccharide to the acid solution is 5.1g:180mL; the acid solution is prepared by mixing citric acid and deionized water at a mass ratio of 1g:30mL;

[0051] In step 2), the dosage ratio of oleic acid, ammonium cerium nitrate solution, anhydrous ethanol, mixed solution and cupric chloride solution is 3.3g:0.61g:22mL:350g:42g; the concentration of the ammonium cerium nitrate solution is 0.02g / mL, and the solvent is deionized water; the cupric chloride solution is prepared by mixing cupric chloride and deionized water in a mass ratio of 2.5:32; the heating treatment is stirring at 38°C for 4.5h; the washing is washing 3 times with anhydrous ethanol; the stirring and mixing time is 50min; the standing time is 3.5h; and the drying is drying at 42°C to constant weight.

[0052] The method for preparing the fermentation product comprises the following steps:

[0053] 1) Weigh straw powder and deionized water, stir and mix for 50 minutes, then add humic acid and mix evenly to obtain a base material; the straw powder is obtained by crushing, grinding and drying straw, and the straw is rice; the mass ratio of the straw powder, deionized water and humic acid is 21:22:50;

[0054] 2) After the fermentation agent and deionized water are evenly mixed, the mixture is sprayed on the base material, fermented for 9 days, and dried until the water content is less than 15wt% to obtain a fermentation product; the mass ratio of the base material, the fermentation agent and the deionized water is 100:1.1:15.3; the fermentation agent is a mixed agent of yeast, Streptomyces griseus and actinomycetes, and the amount of live yeast in the fermentation agent is 1.2×10 8 cfu / mL, the number of live Streptomyces griseus was 1×10 8 cfu / mL, the amount of live actinomycetes is 1×10 8 cfu / mL.

[0055] A method for preparing an organic fertilizer containing fish protein peptide comprises the following steps: uniformly mixing nitrogen, phosphorus and potassium fertilizers, fermentation products, antibacterial agents, medium and trace elements and fish protein peptide, and granulating to obtain the organic fertilizer.

[0056] Example 3

[0057] An organic fertilizer containing fish protein peptides comprises the following components by weight:

[0058] 26 parts of nitrogen, phosphorus and potassium fertilizers, 103 parts of fermentation products, 7 parts of antibacterial agents, 4 parts of trace elements and 33 parts of fish protein peptides;

[0059] The nitrogen, phosphorus and potassium fertilizer is prepared by mixing ammonium nitrate, urea and potassium dihydrogen phosphate in a weight ratio of 2.7:1.3:1.8;

[0060] The trace elements are calcium, magnesium, sulfur, silicon, iron, boron, manganese and zinc, and each of the above elements is in equal amounts;

[0061] The preparation method of the antibacterial agent comprises the following steps:

[0062] 1) stirring and mixing the chitosan oligosaccharide and the acid solution, and then performing ultrasonic treatment to obtain a mixed solution;

[0063] 2) Under a nitrogen atmosphere, oleic acid, ammonium cerium nitrate solution and anhydrous ethanol are mixed and stirred evenly, and then slowly added to the mixture for heating treatment, acetone is added for precipitation, and then the solid is filtered out, immersed in a cupric chloride solution, stirred and mixed, and allowed to stand, and the solid is placed in an oven for drying to obtain an antibacterial agent;

[0064] In step 1), the ultrasonic treatment is performed at a frequency of 37KHz and a power of 330W for 18 minutes; the ratio of the chitosan oligosaccharide to the acid solution is 5.1g:180mL; the acid solution is prepared by mixing citric acid and deionized water at a mass ratio of 1g:30mL;

[0065] In step 2), the amount ratio of oleic acid, ammonium cerium nitrate solution, anhydrous ethanol, mixed solution and cupric chloride solution is 3.5g:0.61g:23mL:355g:43g; the concentration of the ammonium cerium nitrate solution is 0.02g / mL, and the solvent is deionized water; the cupric chloride solution is prepared by mixing cupric chloride and deionized water in a mass ratio of 3:33; the heating treatment is stirring at 42°C for 4.5h; the washing is washing 3 times with anhydrous ethanol; the stirring and mixing time is 55min; the standing time is 4.5h; and the drying is drying at 43°C to constant weight.

[0066] The method for preparing the fermentation product comprises the following steps:

[0067] 1) Weigh straw powder and deionized water, stir and mix for 55 minutes, then add humic acid and mix evenly to obtain a base material; the straw powder is obtained by crushing, grinding and drying straw, and the straw is rice; the mass ratio of the straw powder, deionized water and humic acid is 22:23:50;

[0068] 2) After the fermentation agent and deionized water are evenly mixed, the mixture is sprayed on the base material, fermented for 12 days, and dried until the water content is less than 15wt% to obtain a fermentation product; the mass ratio of the base material, the fermentation agent and the deionized water is 100:1.1:15.7; the fermentation agent is a mixed agent of yeast, Streptomyces griseus and actinomycetes, and the amount of live yeast in the fermentation agent is 1.2×10 8 cfu / mL, the number of live Streptomyces griseus was 1×10 8 cfu / mL, the amount of live actinomycetes is 1×10 8 cfu / mL.

[0069] A method for preparing an organic fertilizer containing fish protein peptide comprises the following steps: uniformly mixing nitrogen, phosphorus and potassium fertilizers, fermentation products, antibacterial agents, medium and trace elements and fish protein peptide, and granulating to obtain the organic fertilizer.

[0070] Example 4

[0071] An organic fertilizer containing fish protein peptides comprises the following components by weight:

[0072] 30 parts of nitrogen, phosphorus and potassium fertilizers, 110 parts of fermentation products, 8 parts of antibacterial agents, 5 parts of trace elements and 40 parts of fish protein peptides;

[0073] The nitrogen, phosphorus and potassium fertilizer is prepared by mixing ammonium nitrate, urea and potassium dihydrogen phosphate in a weight ratio of 3:1.5:2.0;

[0074] The trace elements are calcium, magnesium, sulfur, silicon, iron, boron, manganese and zinc, and each of the above elements is in equal amounts;

[0075] The preparation method of the antibacterial agent comprises the following steps:

[0076] 1) stirring and mixing the chitosan oligosaccharide and the acid solution, and then performing ultrasonic treatment to obtain a mixed solution;

[0077] 2) Under a nitrogen atmosphere, oleic acid, ammonium cerium nitrate solution and anhydrous ethanol are mixed and stirred evenly, and then slowly added to the mixture for heating treatment, acetone is added for precipitation, and then the solid is filtered out, immersed in a cupric chloride solution, stirred and mixed, and allowed to stand, and the solid is placed in an oven for drying to obtain an antibacterial agent;

[0078] In step 1), the ultrasonic treatment is performed at a frequency of 40 KHz and a power of 350 W for 20 min; the ratio of the chitosan oligosaccharide to the acid solution is 5.2 g:180 mL; the acid solution is prepared by mixing citric acid and deionized water at a mass ratio of 1 g:30 mL;

[0079] In step 2), the dosage ratio of oleic acid, ammonium cerium nitrate solution, anhydrous ethanol, mixed solution and cupric chloride solution is 3.6g:0.62g:25mL:360g:45g; the concentration of the ammonium cerium nitrate solution is 0.02g / mL, and the solvent is deionized water; the cupric chloride solution is prepared by mixing cupric chloride and deionized water in a mass ratio of 3.5:35; the heating treatment is stirring at 45°C for 5h; the washing is washing 3 times with anhydrous ethanol; the stirring and mixing time is 60min; the standing time is 5h; and the drying is drying at 45°C to constant weight.

[0080] The method for preparing the fermentation product comprises the following steps:

[0081] 1) Weigh straw powder and deionized water, stir and mix for 60 minutes, then add humic acid and mix evenly to obtain a base material; the straw powder is obtained by crushing, grinding and drying straw, and the straw is rice; the mass ratio of the straw powder, deionized water and humic acid is 23:25:50;

[0082] 2) After the fermentation agent and deionized water are evenly mixed, the mixture is sprayed on the base material, fermented for 14 days, and dried until the water content is less than 15wt% to obtain a fermentation product; the mass ratio of the base material, the fermentation agent and the deionized water is 100:1.2:16; the fermentation agent is a mixed agent of yeast, Streptomyces griseus and actinomycetes, and the amount of live yeast in the fermentation agent is 1.2×10 8 cfu / mL, the number of live Streptomyces griseus was 1×10 8 cfu / mL, the amount of live actinomycetes is 1×10 8 cfu / mL.

[0083] A method for preparing an organic fertilizer containing fish protein peptide comprises the following steps: uniformly mixing nitrogen, phosphorus and potassium fertilizers, fermentation products, antibacterial agents, medium and trace elements and fish protein peptide, and granulating to obtain the organic fertilizer.

[0084] Comparative Example 1

[0085] Compared with Example 3, the difference in Comparative Example 1 is that oleic acid is not added in Comparative Example 1, and the other components, preparation steps and parameters are the same.

[0086] Comparative Example 2

[0087] Compared with Example 3, the difference in Comparative Example 2 is that chitosan oligosaccharide is not added in Comparative Example 2, and the other components, preparation steps and parameters are the same.

[0088] Comparative Example 3

[0089] Compared with Example 3, the difference of Comparative Example 3 is that deionized water is used instead of the copper chloride solution in Comparative Example 3, and the other components, preparation steps and parameters are the same.

[0090] Comparative Example 4

[0091] Compared with Example 3, the difference in Comparative Example 4 is that deionized water is used instead of ammonium cerium nitrate solution, and the other components, preparation steps and parameters are the same.

[0092] The organic fertilizers prepared in Examples 1-4 and Comparative Examples 1-4 were tested as follows, and the test results are shown in Table 1.

[0093] Place the organic fertilizer in a high temperature and high humidity incubator (temperature set at 38°C, relative humidity maintained at 80%) and seal it for storage. Check the samples daily to observe whether there is mildew. Once mold hyphae growth is found in the treatment group, record that day as the number of days of mildew prevention effect (the longer the number of days of mildew prevention, the better the mildew prevention effect).

[0094] Table 1 Anti-mildew test results

[0095]

[0096] From the test results in Table 1, it can be seen that compared with Comparative Examples 1-4, the organic fertilizers prepared in Examples 1-4 of the present invention have excellent anti-mildew effects.

[0097] Antibacterial experiment: Escherichia coli and Staphylococcus aureus were cultured to the logarithmic phase and diluted to 10 5 CFU / mL, use a spreader to evenly spread the diluted bacterial solution on the surface of the solid culture medium. Then, place the Oxford cup vertically on the culture medium and add the sample to be tested into the cup until it is full. Then culture it at 37°C for 16 hours. After the culture is completed, use a vernier caliper to accurately measure the diameter of the inhibition zone. The test results are shown in Table 2.

[0098] Table 2 Antibacterial effect test

[0099]

[0100] From the test results in Table 2, it can be seen that compared with Comparative Examples 1-4, the organic fertilizers prepared in Examples 1-4 have excellent antibacterial effects.

[0101] Growth experiment:

[0102] 300 g of organic fertilizer (Example, Comparative Example) was mixed with soil and potted respectively; after the corn seedlings grew two true leaves, two plants with the same growth were selected in each treatment and transplanted into pots containing fertilizers. After 2 months, the distance from the ground to the top of the corn plant was measured with a tape measure as its plant height (mean); the root length of the plant was selected as its root length (mean); the measurement results are shown in Table 3.

[0103] Table 3 Growth experiment results

[0104]

[0105] The test results are shown in Table 3. It can be seen from the table that the organic fertilizer prepared by the preparation method of the present invention has good fertilizer effect.

[0106] The above description is only a preferred embodiment of the present invention and does not limit the present invention in any form. Although the present invention has been disclosed as a preferred embodiment as above, it is not used to limit the present invention. Any technical personnel in this field can make some changes or modify the technical contents disclosed above into equivalent embodiments without departing from the scope of the technical solution of the present invention. However, any brief modifications, equivalent changes and modifications made to the above embodiments based on the technical essence of the present invention without departing from the content of the technical solution of the present invention are still within the scope of the technical solution of the present invention.

Claims

1. An organic fertilizer containing fish protein peptide, characterized in that: By weight, it includes the following components: 18-30 parts of nitrogen, phosphorus and potassium fertilizers, 90-110 parts of fermentation products, 5-8 parts of antibacterial agents, 2-5 parts of trace elements and 20-40 parts of fish protein polypeptides; The preparation method of the antibacterial agent comprises the following steps: 1) stirring and mixing the chitosan oligosaccharide and the acid solution, and then performing ultrasonic treatment to obtain a mixed solution; 2) Under a nitrogen atmosphere, oleic acid, ammonium cerium nitrate solution and anhydrous ethanol are mixed and stirred evenly, and then slowly added to the mixture for heating treatment, acetone is added for precipitation, and then the solid is filtered out, immersed in a cupric chloride solution, stirred and mixed, and allowed to stand, and the solid is placed in an oven for drying to obtain an antibacterial agent; In step 1), the ultrasonic treatment is performed at a frequency of 30-40KHz and a power of 300-350W for 15-20min; the dosage ratio of the chitosan oligosaccharide to the acid solution is 5.0-5.2g:180mL; the acid solution is prepared by mixing citric acid and deionized water at a mass ratio of 1g:30mL; In step 2), the dosage ratio of oleic acid, ammonium cerium nitrate solution, anhydrous ethanol, mixed solution and cupric chloride solution is 3.2-3.6g: 0.60-0.62g: 20-25mL: 350-360g: 40-45g; the concentration of ammonium cerium nitrate solution is 0.02g / mL, and the solvent is deionized water; the cupric chloride solution is prepared by mixing cupric chloride and deionized water in a mass ratio of 2-3.5: 30-35; the heating treatment is stirring at 35-45°C for 4-5h; the washing is washing 3 times with anhydrous ethanol; the stirring and mixing time is 45-60min; the standing time is 3-5h; the drying is drying at 40-45°C to constant weight; The method for preparing the fermentation product comprises the following steps: 1) Weigh straw powder and deionized water, stir and mix for 45-60 minutes, then add humic acid and mix evenly to obtain base material; 2) After the fermentation bacteria agent and deionized water are evenly mixed, the mixture is sprayed on the base material, fermented for 7-14 days, and dried until the water content is less than 15wt% to obtain a fermentation product; The straw powder is obtained by crushing, grinding and drying the straw, and the straw is one or more of rice, wheat, corn, soybean and sorghum straw; The mass ratio of the straw powder, deionized water and humic acid is 20-23:20-25:50; The mass ratio of the base material, fermentation agent and deionized water is 100:1.0-1.2:15-16; the fermentation agent is a mixed agent of yeast, Streptomyces griseus and actinomycetes, and the amount of live yeast in the fermentation agent is 1.2×10 8 cfu / mL, the number of live Streptomyces griseus was 1×10 8 cfu / mL, the amount of live actinomycetes is 1×10 8 cfu / mL.

2. An organic fertilizer containing fish protein peptide according to claim 1, characterized in that: The nitrogen, phosphorus and potassium fertilizer is prepared by mixing ammonium nitrate, urea and potassium dihydrogen phosphate in a weight ratio of 2-3:1-1.5:1.5-2.

0.

3. An organic fertilizer containing fish protein peptide according to claim 1, characterized in that: The trace elements are calcium, magnesium, sulfur, silicon, iron, boron, manganese and zinc, and the above elements are in equal amounts.

4. A method for preparing an organic fertilizer containing fish protein peptide according to any one of claims 1 to 3, characterized in that: The preparation method comprises the following steps: uniformly mixing nitrogen, phosphorus and potassium fertilizers, fermentation products, antimicrobial agents, medium and trace elements and fish protein polypeptides, and granulating to obtain the product.

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