An automated bioreaction method and system coupled with liquid-solid fermentation

Abstract

The present invention discloses an automated bioreaction method and system for coupled liquid-solid fermentation, including the following steps: establishing a combined fermentation reactor to separately process biological activity in a solid matrix and chemical changes in a liquid, adding a material flow channel with built-in heating and cooling functions; based on a PID algorithm, temperature monitoring of the channel is performed, and reaction substances generated by the combined fermentation reactor during the fermentation process are refluxed into the combined fermentation reactor through a selective reverse circulation mechanism; the present invention establishes a combined fermentation reactor, optimizes reaction conditions and improves bioconversion efficiency; the introduction of the material flow channel with built-in heating and cooling functions not only solves the problem of temperature control, but also improves process stability and reaction efficiency based on temperature monitoring of the PID algorithm; the selective reverse circulation mechanism realizes efficient reflux of reaction substances generated during the fermentation process, which not only enhances substrate utilization but also significantly improves the efficiency and quality of the final fermentation.

Description

Technical Field

[0001] The present invention relates to the field of bioengineering and bioreaction technology, and in particular to a novel microbial biosynthesis engineering technology system coupled with liquid-state and solid-state fermentation and an intelligent automatic control system. Background Art

[0002] In modern bioengineering, solid-state fermentation (SSF) and submerged fermentation (SMF) are two major microbial engineering reaction technology systems, each with extensive applications in pharmaceutical production, the food industry, energy conversion, and other fields. Traditionally, solid-state fermentation involves the fermentation of engineered microbial strains in a solid matrix, which can be a culture medium or other carrier, with nutrients or substrates often present in the solid matrix. Submerged fermentation, on the other hand, involves the fermentation of engineered microbial strains in a liquid fluid matrix, with the culture medium or substrate present in a dissolved state. Both solid-state and submerged fermentation systems have their own advantages and disadvantages, and each has a long history of industrial development and exploitation in different industries.

[0003] Solid-state fermentation, with its thousands of years of history, has been widely used in the food industry for fermentation of fermented koji, sauces, vinegar, and other food products, as well as in traditional Chinese herbal medicine fermentation, making significant contributions to human civilization. Since the 20th century, traditional solid-state fermentation has been gradually integrated into modern industrial production and is currently used in a wide range of modern industries, including enzyme preparations, organic acids, antibiotics, vitamins, biopesticides, biofeed, biopesticides, and the fermentation of Chinese herbal medicines. Solid-state fermentation offers the following advantages: 1) inexpensive raw materials, simple equipment, and low fermentation investment; 2) simple downstream processes, especially when product drying is required, as the substrate moisture content is approximately 50%, making the drying process easy and cost-effective; 3) solid-state fermentation offers unparalleled cost advantages in industries where product purification is not required, such as functional foods, functional probiotics, feed additives, biofeed, biopesticides, and bioenzymes. However, due to the use of solid substrates, solid-state fermentation has inherent deficiencies in material agitation, mixing, aeration and oxygen transfer efficiency, temperature, pH, and moisture control. This leads to uneven distribution of materials, heat conduction, and nutrients, resulting in unstable fermentation conditions. This poses significant obstacles to product quality control and further engineering and industrialization, and also poses challenges to online detection and automated control, as well as the automation and intelligent innovation of solid-state fermentation. To achieve the modernization and upgrading of solid-state fermentation, it is necessary to overcome the above-mentioned drawbacks through innovation in equipment and control software. Through the application of modern tools such as mathematical models and online detection technology, we can further grasp the laws of solid-state fermentation supported by new technologies, and ultimately achieve this through the design of new solid-state fermentation reactors and the upgrading of industrial technology systems.

[0004] Liquid fermentation is a large-scale fermentation technology system developed in the 20th century. It has evolved through the initial stages of liquid fermentation, initiated by pure bacterial culture in the early 20th century, followed by the antibiotic industry phase in the 1940s, driven by the development of microbial physiology and the advancement of submerged aerated stirred fermentation, and the subsequent phases of directed breeding and synthetic biology in the late 20th and early 20th centuries, driven by strain genetic modification, targeted metabolic control, and modern molecular biology techniques. Leveraging modern biotechnology, liquid fermentation facilitates engineering, digitalization, and intelligent control for large-scale, modern production. Today, liquid fermentation has become a mainstream component of the fermentation industry, with microbial metabolites such as amino acids, peptides, high-end bioenzymes, antibiotics, hormones, and many other high-value products primarily produced via large-scale submerged liquid fermentation. Compared to solid-state fermentation, liquid fermentation is significantly more expensive in terms of culture medium, tank equipment, and even fermentation system configuration. High-density liquid fermentation often faces numerous challenges, including limited dissolved oxygen supply, energy-intensive agitation requirements, substrate concentration control, feed pressure, and feedback inhibition caused by metabolite accumulation. These metabolites require supporting downstream extraction, separation and purification processes such as filtration, concentration, extraction, chromatography, crystallization and drying. The cost of downstream separation and purification often far exceeds the upstream fermentation cost.

[0005] In summary, both solid-state fermentation and liquid-phase fermentation demonstrate significant advantages across diverse industries. Integrating or fusing these advantages would not only significantly improve the production efficiency of traditional industries such as functional foods, functional probiotics, feed additives, bio-feed, bio-pesticides, and bio-enzymes, but would also significantly reduce operational pressure downstream of high-end metabolite production. Unfortunately, to date, the fermentation industry, both domestically and internationally, lacks a comprehensive technical and engineering system that integrates solid-state and liquid-phase fermentation. Technological innovation in coupled solid-state and liquid-phase fermentation is crucial.

[0006] In response to the above problems, the technology of coupling solid-state and liquid-state fermentation has gradually attracted the attention of researchers. This combined technology is not only expected to overcome the limitations of a single fermentation mode, but also enhance the overall efficiency and flexibility of the system by complementing the two fermentation modes. However, existing combined fermentation systems often lack effective process integration and control strategies, resulting in the synergistic effects of material transformation during the fermentation process not being fully developed. In addition, the precise control of temperature and other key environmental parameters, as well as product recovery and recycling strategies, have not been effectively solved in existing technologies. These technical challenges limit the application potential of combined fermentation technology on an industrial scale. Summary of the Invention

[0007] The purpose of this section is to summarize some aspects of the embodiments of the present invention and briefly introduce some preferred embodiments. Some simplifications or omissions may be made in this section and the abstract and title of this application to avoid obscuring the purpose of this section, the abstract and the title of the invention, and such simplifications or omissions should not be used to limit the scope of the present invention.

[0008] In view of the above existing problems, the present invention is proposed. Therefore, the present invention provides an automated bioreaction method coupled with liquid-state and solid-state fermentation to solve the problems raised in the background art.

[0009] In order to solve the above technical problems, the present invention provides the following technical solutions:

[0010] In a first aspect, the present invention provides an automated bioreaction method coupled with liquid-state solid-state fermentation, comprising:

[0011] collecting liquid and solid data, and obtaining biological activity in the solid matrix and chemical changes in the liquid matrix based on the liquid and solid data;

[0012] Establishing a combined fermentation reactor to process the biological activity in the solid matrix and the chemical changes in the liquid state respectively, and adding a material flow channel with built-in heating and cooling functions between the combined fermentation reactors;

[0013] Based on the PID algorithm, the temperature of the channel is monitored, and according to the result of the temperature monitoring, the reaction substances produced by the combined fermentation reactor during the fermentation process are refluxed into the combined fermentation reactor through a selective reverse circulation mechanism.

[0014] As a preferred embodiment of the automated bioreaction method coupled with liquid-state solid-state fermentation according to the present invention, it further comprises:

[0015] Multiple microenvironments are simulated in the combined fermentation reactor, wherein the multiple microenvironments include but are not limited to varying oxygen concentrations, dissolved substance concentrations, humidity, shear forces, and pH values.

[0016] As a preferred embodiment of the automated bioreaction method coupled with liquid-solid fermentation of the present invention, wherein: collecting liquid and solid data, and obtaining biological activity in the solid matrix and chemical changes in the liquid matrix based on the liquid and solid data, comprises:

[0017] A respiratory rate sensor is used to collect solid data, and an amino acid and sugar concentration sensor is used to collect liquid data;

[0018] placing the respiratory rate sensor inside a solid matrix where solid data is generated, and obtaining a gradient change value inside the solid matrix;

[0019] placing the amino acid and sugar concentration sensors on both sides of the fluid dynamics generated by the liquid data to obtain the liquid matrix flow characteristics;

[0020] The influencing parameters on the fermentation yield were determined by using regression analysis method based on the interaction between the gradient change value inside the solid matrix and the flow characteristics of the liquid matrix.

[0021] As a preferred embodiment of the automated bioreaction method of coupling liquid-state and solid-state fermentation of the present invention, a combined fermentation reactor is established to separately process the biological activity in the solid matrix and the chemical changes in the liquid matrix, including:

[0022] The established combined fermentation reactor is represented as:

[0023]

[0024] Among them, P total It is represented as a combined fermentation reactor, including liquid and solid fermentation reactors; P rate represents the fermentation yield; k( t ) is expressed as the fermentation efficiency time decay coefficient; r s (C s (t),θ s ) represents the rate of microbial concentration of the solid substrate under temperature and humidity when the solid fermentation reaction occurs at the current time t; s represents the solid substrate; C s (t) is the microbial concentration in the solid matrix; θ s Expressed as temperature and humidity in solid state; r l (C l (t),θ l ) represents the rate of change of the chemical substance concentration of the liquid substrate under the pH value and dissolved oxygen when the liquid fermentation reaction occurs at the current time t; C l (t) represents the concentration of chemical substances in the liquid matrix; θ l Expressed as pH value and dissolved oxygen in liquid state; r s and r l represent the fermentation reaction rates in the solid and liquid matrices respectively; α represents the gradient change value inside the solid matrix; h represents the flow characteristics of the liquid matrix; g represents the interaction function between α and h; T represents the temperature.

[0025] As a preferred embodiment of the automated bioreaction method for coupled liquid-state and solid-state fermentation according to the present invention, a material flow channel with built-in heating and cooling functions is added between the combined fermentation reactors, comprising:

[0026] A dynamic adjustment mechanism is introduced in the channel, according to the temperature T, θ lpH value, microbial concentration C in solid matrix s (t) and the chemical concentration C in the liquid matrix l (t) regulating the flow of materials in the channel;

[0027] Designing the activation conditions of the heating and cooling functions;

[0028] Construct the function of fermentation efficiency and fermentation time;

[0029] The combined fermentation reactor is redefined by the dynamic adjustment mechanism, the starting conditions of the heating and cooling functions, and the changing function of the fermentation efficiency and the fermentation time.

[0030] As a preferred embodiment of the automated bioreaction method coupled with liquid-state solid-state fermentation according to the present invention, the temperature of the channel is monitored based on a PID algorithm, including:

[0031] Set the proportional P, integral I and differential D, and initialize them;

[0032] Setting a target temperature for fermentation, which is an ideal value for fermentation efficiency;

[0033] If the temperature exceeds the target temperature and fluctuates within 1.5°C above or below the target temperature, the output power of the heating or cooling function is continuously adjusted according to the set proportion P, integral I and differential D. Otherwise, the start conditions of forced heating and cooling are triggered.

[0034] As a preferred embodiment of the automated bioreaction method for coupled liquid-state and solid-state fermentation of the present invention, the reaction materials produced during the fermentation process in the combined fermentation reactor are refluxed to the combined fermentation reactor through a selective reverse circulation mechanism according to the result of temperature monitoring, including:

[0035] The selective reverse circulation mechanism divides the reaction materials produced during the fermentation process into reflux fermentation reaction materials and non-reflux fermentation reaction materials;

[0036] The reflux fermentation reaction material includes unreacted added substrate, partially converted or incompletely consumed intermediates during the fermentation process, and a microbial activity balance;

[0037] The non-reflux fermentation reaction substances include organic acids and alcohols that inhibit the growth of microorganisms and completely consumed minerals or vitamins;

[0038] The non-reflux fermentation reaction material is discarded from the material flow channel, and the reflux fermentation reaction material is refluxed into the combined fermentation reactor.

[0039] In a second aspect, the present invention provides an automated bioreactor system coupled with liquid-state and solid-state fermentation, comprising:

[0040] a liquid-solid data analysis module, configured to collect liquid-state and solid-state data, and obtain biological activity in the solid matrix and chemical changes in the liquid matrix based on the liquid-state and solid-state data;

[0041] a combined fermentation reactor control module, configured to establish a combined fermentation reactor to separately process the biological activity in the solid matrix and the chemical changes in the liquid matrix, and to add a material flow channel with built-in heating and cooling functions between the combined fermentation reactors;

[0042] The automatic temperature control and material reflux module is used to monitor the temperature of the channel based on the PID algorithm. According to the temperature monitoring result, the reaction materials produced by the combined fermentation reactor during the fermentation process are refluxed into the combined fermentation reactor through a selective reverse circulation mechanism.

[0043] In a third aspect, the present invention provides a computer device comprising a memory and a processor, wherein the memory stores a computer program, wherein: the processor implements any step of the above method when executing the computer program.

[0044] In a fourth aspect, the present invention provides a computer-readable storage medium having a computer program stored thereon, wherein: when the computer program is executed by a processor, any step of the above method is implemented.

[0045] Compared with the prior art, the invention has the following beneficial effects: the present invention collects liquid and solid data, obtains the biological activity in the solid matrix and the chemical changes in the liquid matrix based on the liquid and solid data, establishes a combined fermentation reactor, and processes the biological activity in the solid matrix and the chemical changes in the liquid respectively, thereby optimizing the reaction conditions and improving the bioconversion efficiency; the introduction of a material flow channel with built-in heating and cooling functions not only solves the problem of temperature control, but also further improves the process stability and reaction efficiency through precise temperature monitoring based on the PID algorithm; in addition, the design of a selective reverse circulation mechanism realizes the efficient reflux of the reaction substances generated during the fermentation process, which not only enhances the utilization rate of the substrate, but also significantly improves the efficiency and quality of the final fermentation. BRIEF DESCRIPTION OF THE DRAWINGS

[0046] To more clearly illustrate the technical solutions of the embodiments of the present invention, the following briefly introduces the drawings required for describing the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. Those skilled in the art can also derive other drawings based on these drawings without inventive effort. Among them:

[0047] Figure 1 This is an overall flow chart of an automated bioreaction method coupled with liquid-state solid-state fermentation according to one embodiment of the present invention;

[0048] Figure 2 This is a schematic diagram of the framework of an automated bioreaction method coupled with liquid-state and solid-state fermentation according to one embodiment of the present invention;

[0049] Figure 3 A temperature control comparison diagram of the automated bioreaction method coupled with liquid-state solid-state fermentation according to one embodiment of the present invention;

[0050] Figure 4 This is a comparison chart of the material reflux product efficiency of the automated bioreaction method coupled with liquid-state solid-state fermentation according to one embodiment of the present invention;

[0051] Figure 5 This is a comparison chart of the remaining amount of substrate in the automated bioreaction method coupled with liquid-state solid-state fermentation according to one embodiment of the present invention. DETAILED DESCRIPTION

[0052] To make the above-mentioned objects, features, and advantages of the present invention more clearly understood, the following detailed description of the specific embodiments of the present invention is given in conjunction with the accompanying drawings. It is obvious that the described embodiments are only part of the embodiments of the present invention, but not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary persons in this field without creative work should fall within the scope of protection of the present invention.

[0053] In the following description, many specific details are set forth to facilitate a full understanding of the present invention. However, the present invention may also be implemented in other ways different from those described herein. Those skilled in the art may make similar generalizations without violating the connotation of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0054] Secondly, the term "one embodiment" or "embodiment" herein refers to a specific feature, structure, or characteristic that may be included in at least one implementation of the present invention. The phrase "in one embodiment" appearing in various places throughout this specification does not necessarily refer to the same embodiment, nor does it refer to a separate or selective embodiment that is mutually exclusive of other embodiments.

[0055] The present invention is described in detail with reference to schematic diagrams. For ease of illustration, cross-sectional views of device structures may be partially enlarged and not to scale when describing embodiments of the present invention. Furthermore, the schematic diagrams are merely illustrative and should not limit the scope of the present invention. Furthermore, in actual production, the three-dimensional dimensions of length, width, and depth should be included.

[0056] In the description of the present invention, it should be noted that the terms "upper, lower, inner, and outer" and other references to orientations or positional relationships are based on the orientations or positional relationships shown in the accompanying drawings and are intended solely to facilitate and simplify the description of the present invention. They are not intended to indicate or imply that the devices or components referred to must have, be constructed, or operate in a specific orientation, and therefore should not be construed as limitations on the present invention. Furthermore, the terms "first, second, or third" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0057] In this disclosure, unless otherwise specified or limited, the terms "mounted," "connected," and "connected" should be interpreted broadly. For example, they may refer to fixed, removable, or integral connections. They may also refer to mechanical, electrical, or direct connections, indirect connections through an intermediary, or internal communication between two components. Those skilled in the art will understand the specific meanings of these terms in this disclosure.

[0058] Example 1

[0059] Reference Figure 1 and Figure 2 , which is the first embodiment of the present invention, provides an automated bioreaction method coupled with liquid-state solid-state fermentation, comprising:

[0060] S1. Collect liquid and solid data, and obtain the biological activity in the solid matrix and the chemical changes in the liquid matrix based on the liquid and solid data;

[0061] Furthermore, a respiratory rate sensor is used to collect solid-state data, and an amino acid and sugar concentration sensor is used to collect liquid-state data;

[0062] Specifically, the respiratory rate sensor is a sensor used to measure biological activity, and the amino acid and sugar concentration sensor is a nutrient-specific sensor;

[0063] Specifically, liquid data include: pH value, dissolved oxygen content, nutrient concentration, organic acid content, alcohol content, and sugar consumption rate;

[0064] Specifically, solid-state data include: water activity, microbial growth (such as colony counts), temperature distribution, pH changes, metabolites (such as enzyme activity), etc.

[0065] It should be noted that the sensor needs to be calibrated before being used to collect solid and liquid data;

[0066] Furthermore, a respiratory rate sensor is placed inside a solid matrix where solid data is generated, and a gradient change value inside the solid mechanism is obtained;

[0067] Furthermore, amino acid and sugar concentration sensors were placed on both sides of the fluid dynamics generated by the liquid data to obtain the liquid matrix flow characteristics;

[0068] Specifically, for liquid matrices, it is necessary to select areas with strong convection to ensure that the sensors on both sides can capture representative sample data without affecting the flow characteristics of the liquid matrix;

[0069] Furthermore, the interaction between the gradient change value inside the solid matrix and the flow characteristics of the liquid matrix was analyzed using regression analysis to determine the parameters affecting the fermentation yield.

[0070] Specifically, the fermentation yield P rate Expressed as:

[0071]

[0072] Among them, C s (t) is expressed as the microbial concentration of the solid matrix; C l (t) represents the chemical concentration of the liquid matrix; T represents the temperature; h represents the flow characteristics of the liquid matrix; T s represents the bulk temperature of the solid matrix; T l represents the overall temperature of the liquid matrix; g represents the interaction function of α and h; f represents the temperature normalization function; k represents the reaction sensitivity coefficient, which is used to adjust the influence of microbial activity and chemical changes on fermentation yield;

[0073] Specifically, Represents a nonlinear combination of solid and liquid parameters to express the complexity of the interaction between the two matrices;

[0074] Specifically, The temperature is converted into a standard form through the temperature standardization function to facilitate the internal calculation of the combined fermentation reactor; T min Indicates the lower temperature threshold; T max Indicates the upper threshold of temperature;

[0075] Specifically, h(f(T s ),f(T l ))=f(T s ) 2 +f(T l ) 2 ―2f(T s )f(T l ); The nonlinear characteristics of the effect of temperature difference on fermentation are expressed in the form of square sum difference;

[0076] It should be noted that regression analysis can quantify the gradient changes in the solid matrix (such as temperature, humidity, pH, etc.) and the flow of the liquid matrix (such as flow rate, flow pattern), and maximize the fermentation yield at a certain flow rate, thereby accurately controlling the fermentation conditions and maximizing the conversion rate of the raw materials.

[0077] S2. Establish a combined fermentation reactor to process the biological activity in the solid matrix and the chemical changes in the liquid respectively, and add a material flow channel with built-in heating and cooling functions between the combined fermentation reactors;

[0078] Further, the established combined fermentation reactor is expressed as:

[0079]

[0080] Among them, P total It represents a combined fermentation reactor, including liquid and solid fermentation reactors; k(t) represents the fermentation efficiency time decay coefficient; r s (C s (t),θ s ) represents the rate of microbial concentration of the solid substrate under temperature and humidity when the solid fermentation reaction occurs at the current time t; s represents the solid substrate; θ s Expressed as temperature and humidity in solid state; r l (C l (t),θ l ) represents the rate of chemical concentration of liquid substrate under pH value and dissolved oxygen when liquid fermentation reaction occurs at the current time t; θ l Expressed as pH value and dissolved oxygen in liquid state; r s and r l represent the fermentation reaction rates in solid and liquid matrices respectively; α represents the gradient change value inside the solid matrix;

[0081] Specifically,

[0082] Specifically, l (C l (t),θ l )=C l ×exp(―θ l );

[0083] Specifically,

[0084] Furthermore, multiple microenvironments are simulated in the combined fermentation reactor, wherein the multiple microenvironments include but are not limited to varying oxygen concentrations, dissolved substance concentrations, humidity, shear stress, and pH values;

[0085] Specifically, the liquid and solid fermentation reactors respectively include independent liquid fermentation units and solid fermentation units; each fermentation unit includes a high-efficiency stirring device and a gas distribution system to ensure uniform mixing of the medium and sufficient supply of oxygen;

[0086] It should be noted that by establishing a combined fermentation reactor, the processing of coupled solid and liquid substrates and the utilization of g(C s (t),C l (t)) on the reactants, achieving more precise control of the fermentation process and optimizing the fermentation conditions;

[0087] Furthermore, a dynamic adjustment mechanism is introduced into the channel, according to the temperature T, θ l pH value, microbial concentration C in solid matrix s (t) and the chemical concentration C in the liquid matrix l (t) regulating the flow of materials in the channel;

[0088] Specifically, the dynamic adjustment mechanism D(t) is expressed as:

[0089]

[0090] Among them, T opt and pH opt They are represented by the preset temperature value and the preset pH value respectively; a, b, c, d correspond to the weights of the parameters, that is, temperature T, θ l pH value, microbial concentration C in solid matrix s (t) and the chemical concentration C in the liquid matrix l (t);

[0091] Going a step further, design the activation conditions of the heating and cooling functions;

[0092] Specifically, the starting conditions of the heating and cooling functions are expressed as follows:

[0093]

[0094] Where H(T) represents the heating or cooling start function, 1 represents a successful start, and 0 represents a failed start;

[0095] Furthermore, a function of the change of fermentation efficiency and fermentation time is constructed;

[0096] It should be noted that, considering that the fermentation efficiency may change over time, especially in the long-term fermentation process, we introduced the time decay factor γ(t) corresponding to the previous k(t);

[0097] Specifically, the function of fermentation efficiency and fermentation time is expressed as:

[0098]

[0099] Among them, β is the prediction parameter, which represents the influence of past fermentation performance on future fermentation performance;

[0100] Furthermore, the combined fermentation reactor is redefined through the dynamic adjustment mechanism, the start-up conditions of the heating and cooling functions, and the changing function of fermentation efficiency and fermentation time;

[0101] Specifically, the redefined combined fermentation reactor is:

[0102]

[0103] S3. Based on the PID algorithm, the temperature of the channel is monitored. According to the temperature monitoring result, the reaction substances produced during the fermentation process of the combined fermentation reactor are refluxed to the combined fermentation reactor through a selective reverse circulation mechanism;

[0104] It should be noted that designing the start-up conditions of the heating and cooling functions can only solve the temperature control problem, but does not achieve the effect of monitoring the temperature, so the PID algorithm is needed for temperature monitoring;

[0105] Specifically, the channel can be divided into three sections: the entrance, the middle section, and the exit section;

[0106] Furthermore, the proportional P, integral I and differential D are set and initialized;

[0107] Specifically, the purpose of setting these parameters is to adjust the response intensity and speed of the heating and cooling functions to maintain the target temperature required for fermentation;

[0108] Furthermore, a target temperature for fermentation is set, which is an ideal value for fermentation efficiency;

[0109] Specifically, the ideal value of fermentation efficiency can be obtained by rate The maximum value of is obtained;

[0110] Specifically, the PID algorithm is set to read the temperatures at the inlet, middle section, and outlet in sequence at regular intervals;

[0111] Furthermore, if the temperature exceeds the target temperature and fluctuates within 1.5°C above or below the target temperature, the activation conditions of the heating and cooling functions are triggered; otherwise, the output power of the heating or cooling function is continuously adjusted according to the set proportional P, integral I and differential D;

[0112] Specifically, the output power is fed back to the heating and cooling start-up conditions in the channel, ensuring that the temperature is maintained within the optimal fermentation range through a continuous feedback loop;

[0113] Specifically, the accuracy of the PID algorithm is evaluated regularly (e.g., monthly or quarterly), and the parameters in the PID algorithm are adjusted according to the actual collected output power to optimize the accuracy of temperature control;

[0114] Furthermore, a selective reverse circulation mechanism is provided to separate the reaction materials produced during the fermentation process into reflux fermentation reaction materials and non-reflux fermentation reaction materials;

[0115] Furthermore, the reflux fermentation reaction materials include unreacted added substrates, partially converted or incompletely consumed intermediates during the fermentation process, and the balance of microbial activity;

[0116] Specifically, unreacted added substrates such as sugars, amino acids, vitamins, etc. that are not completely consumed during the fermentation process are refluxed through the material flow channel;

[0117] Specifically, the intermediates that are partially converted or not completely consumed during the fermentation process refer to organic acids, alcohols or enzymes, etc. These are usually partially converted or not completely consumed during the fermentation process and can also be refluxed at this time;

[0118] Specifically, the microbial activity balance refers to the use of chemicals such as sodium carbonate to adjust the pH value during the fermentation process, and the excess production of such substances that are beneficial to microbial activity is returned to the combined reactor;

[0119] Furthermore, the non-reflux fermentation reaction substances include organic acids and alcohols that inhibit the growth of microorganisms and completely consumed minerals or vitamins;

[0120] Furthermore, the non-refluxed fermentation reaction material is discarded from the material flow channel, and the refluxed fermentation reaction material is refluxed into the combined fermentation reactor;

[0121] It should be noted that the reflux mechanism not only optimizes the fermentation resources in the combined reactor, but also enhances the quality and efficiency of the final fermentation product and reduces resource waste.

[0122] Furthermore, this embodiment also provides an automated bioreactor system coupled with liquid-state and solid-state fermentation, comprising:

[0123] a liquid-solid data analysis module, configured to collect liquid-state and solid-state data, and obtain biological activity in the solid matrix and chemical changes in the liquid matrix based on the liquid-state and solid-state data;

[0124] a combined fermentation reactor control module, configured to establish a combined fermentation reactor to separately process the biological activity in the solid matrix and the chemical changes in the liquid matrix, and to add a material flow channel with built-in heating and cooling functions between the combined fermentation reactors;

[0125] The automatic temperature control and material reflux module is used to monitor the temperature of the channel based on the PID algorithm. According to the temperature monitoring result, the reaction materials produced by the combined fermentation reactor during the fermentation process are refluxed into the combined fermentation reactor through a selective reverse circulation mechanism.

[0126] This embodiment further provides a computer device suitable for coupling an automated bioreaction method of liquid-state and solid-state fermentation, comprising:

[0127] Memory and processor; the memory is used to store computer-executable instructions, and the processor is used to execute computer-executable instructions to implement the automated biological reaction method coupled with liquid-solid fermentation as proposed in the above embodiment.

[0128] The computer device may be a terminal, comprising a processor, a memory, a communication interface, a display screen and an input device connected via a system bus. The processor of the computer device is used to provide computing and control capabilities. The memory of the computer device comprises a non-volatile storage medium and an internal memory. The non-volatile storage medium stores an operating system and a computer program. The internal memory provides an environment for the operation of the operating system and the computer program in the non-volatile storage medium. The communication interface of the computer device is used to communicate with an external terminal in a wired or wireless manner, and the wireless manner may be achieved through WIFI, an operator network, NFC (near field communication) or other technologies. The display screen of the computer device may be a liquid crystal display or an electronic ink display screen, and the input device of the computer device may be a touch layer covering the display screen, or a button, trackball or touchpad provided on the housing of the computer device, or an external keyboard, touchpad or mouse, etc.

[0129] This embodiment further provides a storage medium storing a computer program, which, when executed by a processor, implements the automated biological reaction method of coupled liquid-state and solid-state fermentation as proposed in the above embodiment.

[0130] The storage medium proposed in this embodiment and the data storage method proposed in the above embodiment belong to the same inventive concept. Technical details not fully described in this embodiment can be found in the above embodiment, and this embodiment has the same beneficial effects as the above embodiment.

[0131] Example 2

[0132] Reference Figures 3 to 5 , which is the second embodiment of the present invention, provides an automated bioreaction method coupled with liquid-solid fermentation, including: verifying the beneficial effects of the solution of the present invention by means of simulation experiments;

[0133] Experimental preparation: Two fermentation systems were constructed: one using the combined fermentation reactor of the present invention and the other a traditional separate fermentation system. Common biological fermentation feedstocks, including sugars and amino acids, were selected as substrates to ensure consistent input materials for both systems. The respiration rate sensor and amino acid and sugar concentration sensors mentioned in the present invention were installed in each system to monitor key parameters during the fermentation process in real time.

[0134] The experimental process involved starting two fermentation systems and collecting initial data, including preliminary biological activity and chemical changes of solid and liquid substrates. The temperature of the coupled fermentation reactor proposed in the present invention was controlled in real time using a PID algorithm, while temperature fluctuations in a conventional system were recorded. A material recirculation mechanism was implemented in the system of the present invention to recycle some unreacted substrates and intermediate products, while the conventional system did not. The fermentation efficiency and product yield of the two fermentation systems were measured, and their bioconversion efficiencies were compared. Based on feedback from the PID algorithm, the heating and cooling responses in the coupled fermentation system were adjusted to maintain optimal fermentation conditions.

[0135] Experimental results: refer to Table 1 and Table 2;

[0136] Table 1 Comparison between coupled fermentation system and traditional fermentation system

[0137] Parameters / System Combined fermentation reactor Traditional separation fermentation system Fermentation efficiency (%) 95 75 Temperature stability (±℃) 1 5 Substrate conversion rate (%) 90 70 Product yield (g / L) 1.2 0.8 Resource recovery rate (%) 85 No reflux

[0138] As can be seen from Table 1, compared with the traditional separate fermentation system, the combined fermentation reactor of the present invention has significantly improved fermentation efficiency, substrate conversion rate, and product yield. In particular, in terms of temperature stability and resource recovery rate, the present invention is significantly superior to the existing technology through precise PID temperature control and efficient material reflux mechanism.

[0139] Table 2 Real-time monitoring of key parameters during fermentation

[0140]

[0141] As can be seen from the data in Table 2, by continuously tracking the changes in key parameters, the present invention can maintain optimal biological activity and chemical change conditions throughout the fermentation process, thereby improving the overall efficiency and yield of the fermentation;

[0142] Depend on Figure 3 As can be seen, the PID algorithm can significantly reduce temperature fluctuations based on the combined fermentation reactor, maintaining a more stable temperature compared to traditional systems. This stability is due to the proportional, integral, and differential actions of the PID, which can adjust the heating or cooling response of the reactor in real time to accurately control the temperature to the set target.

[0143] pass Figure 4 and Figure 5 It can also be seen that the reflux mechanism of the present invention can reflux unreacted substrates and partially converted intermediates, thereby significantly improving resource utilization efficiency; compared with traditional fermentation systems without reflux, systems with reflux can more effectively use the input substrates and reduce raw material waste.

[0144] In summary, the present invention not only solves some key problems in traditional fermentation technology, such as difficult temperature control and inefficient resource utilization, but also significantly improves the stability and economic benefits of the fermentation process by introducing innovative coupling technology and automated control, fully demonstrating the innovation and practicality of the present invention.

[0145] Those skilled in the art will appreciate that the embodiments of the present invention may be provided as methods, systems, or computer program products. Therefore, the present application may adopt the form of a complete hardware embodiment, a complete software embodiment, or an embodiment in combination with software and hardware. Furthermore, the present application may adopt the form of a computer program product implemented on one or more computer-usable storage media (including but not limited to disk storage, CD-ROM, optical storage, etc.) containing computer-usable program code. The solutions in the embodiments of the present application may be implemented in various computer languages, for example, object-oriented programming language Java and interpreted scripting language JavaScript, etc.

[0146] The present application is described with reference to the flowcharts and / or block diagrams of the methods, devices (systems), and computer program products according to the embodiments of the present application. It should be understood that each process and / or box in the flowchart and / or block diagram, as well as the combination of the processes and / or boxes in the flowchart and / or block diagram, can be implemented by computer program instructions. These computer program instructions can be provided to a processor of a general-purpose computer, a special-purpose computer, an embedded processor, or other programmable data processing device to produce a machine, so that the instructions executed by the processor of the computer or other programmable data processing device generate instructions for implementing the steps in the process. Figure 1 a process or multiple processes and / or boxes Figure 1 A device that provides the functions specified in a block or multiple blocks.

[0147] These computer program instructions may also be stored in a computer readable memory that can direct a computer or other programmable data processing device to work in a specific manner, so that the instructions stored in the computer readable memory produce an article of manufacture comprising an instruction device, which implements the process Figure 1 a process or multiple processes and / or boxes Figure 1 The function specified in one or more boxes.

[0148] These computer program instructions can also be loaded onto a computer or other programmable data processing device so that a series of operational steps are executed on the computer or other programmable device to produce a computer-implemented process, thereby providing the instructions executed on the computer or other programmable device for implementing the process. Figure 1 a process or multiple processes and / or boxes Figure 1 A step that specifies a function in one or more boxes.

[0149] Although the preferred embodiments of the present application have been described, those skilled in the art may make additional changes and modifications to these embodiments once they have learned the basic creative concept. Therefore, the appended claims are intended to be interpreted as including the preferred embodiments and all changes and modifications that fall within the scope of the present application.

[0150] Obviously, those skilled in the art may make various changes and modifications to this application without departing from the spirit and scope of this application. Thus, if these modifications and variations of this application fall within the scope of the claims of this application and their equivalents, this application is intended to include these modifications and variations.

Claims

1. An automated bioreaction method coupled with liquid-solid fermentation, characterized in that: include: collecting liquid and solid data, and obtaining biological activity in the solid matrix and chemical changes in the liquid matrix based on the liquid and solid data; The collecting of liquid and solid data and obtaining biological activity in the solid matrix and chemical changes in the liquid matrix based on the liquid and solid data include: A respiratory rate sensor is used to collect solid data, and an amino acid and sugar concentration sensor is used to collect liquid data; placing the respiratory rate sensor inside a solid matrix where solid data is generated, and obtaining a gradient change value inside the solid matrix; placing the amino acid and sugar concentration sensors on both sides of the fluid dynamics generated by the liquid data to obtain the liquid matrix flow characteristics; The influencing parameters on the fermentation yield were determined by using regression analysis method based on the interaction between the gradient change value inside the solid matrix and the flow characteristics of the liquid matrix. The liquid data include pH value, dissolved oxygen content, nutrient salt concentration, organic acid content, alcohol content and sugar consumption rate; The solid state data includes water activity, microbial growth, temperature distribution, pH changes, and metabolites; Establishing a combined fermentation reactor to process the biological activity in the solid matrix and the chemical changes in the liquid state respectively, and adding a material flow channel with built-in heating and cooling functions between the combined fermentation reactors; The method of establishing a combined fermentation reactor to separately process the biological activity in the solid matrix and the chemical changes in the liquid matrix comprises: The established combined fermentation reactor is represented as: Among them, P total It is represented as a combined fermentation reactor, including liquid and solid fermentation reactors; P rate represents the fermentation yield; k(t) represents the fermentation efficiency time decay coefficient; r s (C s (t),θ s ) represents the rate of microbial concentration of the solid substrate under temperature and humidity when the solid fermentation reaction occurs at the current time t; s represents the solid substrate; C s (t) is the microbial concentration in the solid matrix; θ s Expressed as temperature and humidity in solid state; r l (C l (t),θ l ) represents the rate of change of the chemical substance concentration of the liquid substrate under the pH value and dissolved oxygen when the liquid fermentation reaction occurs at the current time t; C l (t) represents the concentration of chemical substances in the liquid matrix; θ l Expressed as pH value and dissolved oxygen in liquid state; r s and r l represents the fermentation reaction rate in solid and liquid matrices respectively; α represents the gradient change value inside the solid matrix; h represents the flow characteristics of the liquid matrix; g represents the interaction function between α and h; T represents the temperature; Specifically, Specifically, l (C l (t),θ l )=C l ×exp(-θ l ); Specifically, Fermentation yield P rate Expressed as: Among them, C s (t) is expressed as the microbial concentration of the solid matrix; C l (t) represents the chemical concentration of the liquid matrix; T represents the temperature; h represents the flow characteristics of the liquid matrix; T s represents the bulk temperature of the solid matrix; T l represents the overall temperature of the liquid matrix; g represents the interaction function of α and h; f represents the temperature normalization function; k represents the reaction sensitivity coefficient, which is used to adjust the influence of microbial activity and chemical changes on fermentation yield; Specifically, Represents a nonlinear combination of solid and liquid parameters to express the complexity of the interaction between the two matrices; Specifically, The temperature is converted into a standard form through the temperature standardization function to facilitate the internal calculation of the combined fermentation reactor; T min Indicates the lower temperature threshold; T max Indicates the upper threshold of temperature; Specifically, h(f(T s ),f(T l ))=f(T s ) 2 +f(T l ) 2 -2f(T s )f(T l ); The nonlinear characteristics of the effect of temperature difference on fermentation are expressed in the form of square sum difference; Based on the PID algorithm, the temperature of the channel is monitored, and according to the result of the temperature monitoring, the reaction substances produced by the combined fermentation reactor during the fermentation process are refluxed into the combined fermentation reactor through a selective reverse circulation mechanism.

2. The automated bioreaction method coupled with liquid-state solid-state fermentation according to claim 1, characterized in that: Also includes: Multiple microenvironments are simulated in the combined fermentation reactor, wherein the multiple microenvironments include but are not limited to varying oxygen concentrations, dissolved substance concentrations, humidity, shear forces, and pH values.

3. The automated bioreaction method coupled with liquid-state solid-state fermentation according to claim 2, wherein: Between the combined fermentation reactors, a material flow channel with built-in heating and cooling functions is added, including: A dynamic adjustment mechanism is introduced in the channel, according to the temperature T, θ l pH value, microbial concentration C in solid matrix s (t) and the chemical concentration C in the liquid matrix l (t) regulating the flow of materials in the channel; Designing the activation conditions of the heating and cooling functions; Construct the function of fermentation efficiency and fermentation time; The combined fermentation reactor is redefined by the dynamic adjustment mechanism, the starting conditions of the heating and cooling functions, and the changing function of the fermentation efficiency and the fermentation time.

4. The automated bioreaction method coupled with liquid-state solid-state fermentation according to claim 3, wherein: Based on the PID algorithm, the temperature of the channel is monitored, including: Set the proportional P, integral I and differential D, and initialize them; Setting a target temperature for fermentation, which is an ideal value for fermentation efficiency; If the temperature exceeds the target temperature and fluctuates within 1.5°C above or below the target temperature, the output power of the heating or cooling function is continuously adjusted according to the set proportion P, integral I and differential D. Otherwise, the start conditions of forced heating and cooling are triggered.

5. The automated bioreaction method coupled with liquid-state solid-state fermentation according to claim 4, characterized in that: Based on the results of temperature monitoring, the reaction substances produced during the fermentation process in the combined fermentation reactor are refluxed into the combined fermentation reactor through a selective reverse circulation mechanism, including: The selective reverse circulation mechanism divides the reaction materials produced during the fermentation process into reflux fermentation reaction materials and non-reflux fermentation reaction materials; The reflux fermentation reaction material includes unreacted added substrate, partially converted or incompletely consumed intermediates during the fermentation process, and a microbial activity balance; The non-reflux fermentation reaction substances include organic acids and alcohols that inhibit the growth of microorganisms and completely consumed minerals or vitamins; The non-reflux fermentation reaction material is discarded from the material flow channel, and the reflux fermentation reaction material is refluxed into the combined fermentation reactor.

6. An automated bioreactor system coupled with liquid-solid fermentation, based on the automated bioreactor method coupled with liquid-solid fermentation according to any one of claims 1 to 5, characterized in that: include: a liquid-solid data analysis module, configured to collect liquid-state and solid-state data, and obtain biological activity in the solid matrix and chemical changes in the liquid matrix based on the liquid-state and solid-state data; a combined fermentation reactor control module, configured to establish a combined fermentation reactor to separately process the biological activity in the solid matrix and the chemical changes in the liquid matrix, and to add a material flow channel with built-in heating and cooling functions between the combined fermentation reactors; The automatic temperature control and material reflux module is used to monitor the temperature of the channel based on the PID algorithm. According to the temperature monitoring result, the reaction materials produced by the combined fermentation reactor during the fermentation process are refluxed into the combined fermentation reactor through a selective reverse circulation mechanism.

7. A computer device comprising a memory and a processor, wherein the memory stores a computer program, wherein: When the processor executes the computer program, the steps of the method according to any one of claims 1 to 5 are implemented.

8. A computer-readable storage medium having a computer program stored thereon, characterized in that: When the computer program is executed by a processor, the steps of the method according to any one of claims 1 to 5 are implemented.

Images