A composite deodorization method for reducing the fishy smell of Xue's sea dragon peptide powder

Abstract

The invention discloses a composite deodorization method for reducing the fishy smell of Xue's sea dragon peptide powder, and relates to the technical field of aquatic functional peptides. The invention adds Xue's sea dragon peptide powder to a glucose aqueous solution, keeps warm at 40-50°C for 3-5h, and then cools to room temperature; then adds β-cyclodextrin to the reaction system, disperses and homogenizes, and obtains the deodorized Xue's sea dragon peptide powder after drying. The invention deodorizes Xue's sea dragon peptide powder by a glucose-β-cyclodextrin combination method, and through a one-step simple composite deodorization preparation process, Xue's sea dragon peptide powder can be effectively deodorized on the basis of retaining the basic flavor of Xue's sea dragon peptide raw material, and has a wide application prospect for the preparation of Xue's sea dragon peptide powder with low fishy smell. The invention has the characteristics of simple processing method and easy promotion, which is of great significance to promoting the high-value application of Xue's sea dragon peptide powder.

Description

Technical Field

[0001] The invention relates to the technical field of aquatic functional peptides, in particular to a composite deodorization method for reducing the fishy smell of Xue's sea dragon peptide powder. Background Art

[0002] Aquatic protein peptides have the characteristics of good oral absorption and rich active functions, and have been favored by many researchers. Syngnathus is a small marine fish belonging to the class of bony fish in the phylum Chordata. It is an important resource for both medicine and food. Its protein content is between 59.06% and 73.56%, which is rich in content and is a good source for processing as active peptides. At present, studies have shown that Syngnathus peptides have good active functions and are a promising aquatic functional food raw material.

[0003] Schlegeli's sea dragon (Syngnathus schlegeli), belonging to the class of bony fish (Osteichthyes), order of Syngnathiformes, family of Syngnathidae, genus Syngnathus, also known as Schlegeli's sea dragon, is widely distributed in the coastal waters of the northwest Pacific Ocean. Schlegeli's sea dragon is a promising functional food raw material, but it is still used as feed by simple crushing. Its important blue protein resource has yet to be released, and the processing of Schlegeli's sea dragon protein hydrolysate is particularly urgent. At present, Schlegeli's sea dragon peptide powder has a strong inherent fishy smell, which affects its subsequent deep processing application. Therefore, the need to develop an effective method to remove the fishy smell of Schlegeli's sea dragon is particularly urgent, which is of great significance to promote its high-value utilization and related product development. Summary of the invention

[0004] The purpose of the present invention is to provide a composite deodorization method for reducing the fishy smell of Xue's halofopeptide powder, so as to solve the problems existing in the above-mentioned prior art, and on the basis of ensuring the basic flavor of Xue's halofopeptide raw materials, efficiently remove the fishy smell thereof, and promote high-value utilization and related product development.

[0005] To achieve the above object, the present invention provides the following solutions:

[0006] The present invention provides a composite deodorization method for reducing the fishy smell of Xue's haloperidol peptide powder, comprising the following steps:

[0007] The Xue's sea dragon peptide powder is added to the glucose aqueous solution, kept at 40-50°C for 3-5 hours and then cooled to room temperature; then β-cyclodextrin is added to the reaction system for dispersion and homogenization, and the Xue's sea dragon peptide powder after deodorization is obtained after drying.

[0008] Furthermore, the concentration of the glucose aqueous solution is 1-4wt%.

[0009] The amount of β-cyclodextrin added is 25%-100% of the mass of glucose. Further, the homogenization is high-pressure homogenization; the pressure of the high-pressure homogenization is 70-80 MPa.

[0010] Furthermore, the drying temperature is 40-50° C. and the drying time is 8-12 hours.

[0011] Furthermore, the preparation method of the Xue's sea dragon peptide powder comprises the following steps:

[0012] The schizontosaurus is enzymatically hydrolyzed with alkaline protease, the enzyme is inactivated, the supernatant is centrifuged, dialyzed to obtain a fraction less than 3 kDa, and freeze-dried under vacuum conditions to obtain the schizontosaurus peptide powder.

[0013] Furthermore, the temperature of the enzymolysis is 40-60°C, and the time of the enzymolysis is 4-6h.

[0014] More preferably, the composite deodorization method of the present invention is as follows:

[0015] (1) 10 g of Hesperus schrenkiana was crushed, 100 mL of double distilled water was added, the pH was adjusted to 8 using a 1 M sodium hydroxide aqueous solution, and then 100 mg of alkaline protease was added for enzymolysis at 40-60° C. for 4-6 h. Subsequently, the obtained enzymolysis solution was inactivated in a 100° C. boiling water bath for 10 min, centrifuged at 8000 r / min for 15 min, the supernatant was taken, dialyzed with an ultrafiltration membrane, and the fraction less than 3 kDa was taken, and vacuum freeze-dried to obtain Hesperus schrenkiana peptide powder.

[0016] (2) Take 100-150mL of 1-4wt% glucose aqueous solution, add 5-10g of the Xue's sea dragon peptide powder prepared in step (1), and place in a water bath at 40-50°C for 3-5h; then add β-cyclodextrin to the system (the addition amount is 25%-100% of the mass of glucose), disperse at 10000rpm for 1min, high-pressure homogenize at 70-80MPa for 3 times, take 20mL of the uniform solution, and dry with hot air at 40-50°C for 8-12h to obtain the Xue's sea dragon peptide powder after deodorization.

[0017] In the present invention, the addition of β-cyclodextrin has a certain protective effect on the basic flavor substances of the sea dragon peptide powder when it is dried, and retains a small amount of original flavor substances of the sea dragon peptide powder such as mushroom alcohol (mushroom, hay aroma), 2-ethylhexanol (floral fragrance), hexanol (alcohol taste, sweet taste) and toluene (aromatic taste).

[0018] The present invention also provides the Xue's sea dragon peptide powder obtained by the composite fishy removal method.

[0019] The present invention discloses the following technical effects:

[0020] The invention uses a glucose-β-cyclodextrin combined method to remove the fishy smell of Xue's halodendron peptide powder. Through a one-step simple composite fishy smell removal preparation process, the Xue's halodendron peptide powder can be effectively deodorized while retaining the basic flavor of the Xue's halodendron peptide raw material. The invention has a wide application prospect for the preparation of Xue's halodendron peptide powder with low fishy smell.

[0021] The processing method of the invention is simple and easy to promote, and has important significance for promoting the high-value application of Xue's sea dragon peptide powder. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0023] Figure 1 These are radar graphs of the electronic nose sensor of the Hailong peptide powder after deodorization in different treatment groups of Example 1 and Comparative Examples 1-3; (a) is a radar graph of the electronic nose sensor of the Hailong peptide powder and the samples obtained from each treatment group, and (b) is a radar graph of the electronic nose sensor of the samples obtained from each treatment group.

[0024] Figure 2 The changes in the amount of flavor substances in the haloperidol powder before and after deodorization treatment under different treatment methods in Example 1 of the present invention and Comparative Examples 1-3 are shown.

[0025] Figure 3 The distribution of the peak area (a) of the volatile components and the relative internal standard content (b) of each sample under different treatment methods in Example 1 of the present invention and Comparative Examples 1-3. DETAILED DESCRIPTION

[0026] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as limiting the present invention, but should be understood as a more detailed description of certain aspects, features, and embodiments of the present invention.

[0027] It should be understood that the terms described in the present invention are only for describing a particular embodiment and are not intended to limit the present invention. In addition, for the numerical range in the present invention, it should be understood that each intermediate value between the upper and lower limits of the scope is also specifically disclosed. The intermediate value in any stated value or stated range, and each smaller range between any other stated value or intermediate value in the described range is also included in the present invention. The upper and lower limits of these smaller ranges can be independently included or excluded in the scope.

[0028] Unless otherwise indicated, all technical and scientific terms used herein have the same meanings as those generally understood by those skilled in the art. Although the present invention describes only preferred methods and materials, any methods and materials similar or equivalent to those described herein may also be used in the implementation or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials associated with the documents. In the event of a conflict with any incorporated document, the content of this specification shall prevail.

[0029] It will be apparent to those skilled in the art that various modifications and variations may be made to the specific embodiments of the present invention description without departing from the scope or spirit of the present invention. Other embodiments derived from the present invention description will be apparent to those skilled in the art. The present invention description and examples are exemplary only.

[0030] The words “include,” “including,” “have,” “contain,” etc. used in this document are open-ended terms, meaning including but not limited to.

[0031] In the following embodiments and comparative examples of the present invention, the Xue's sea dragon peptide powder used is prepared by the following steps:

[0032] 10 g of Xue's sea dragon was crushed, 100 mL of double distilled water was added, the pH was adjusted to 8 with 1 M sodium hydroxide solution, and then 100 mg of alkaline protease was added for enzymolysis at 50 ° C for 5 h. Subsequently, the obtained enzymatic solution was inactivated in a 100 ° C boiling water bath for 10 min, centrifuged at 8000 r / min for 15 min, the supernatant was taken, dialyzed with an ultrafiltration membrane, and the fraction less than 3 kDa was taken. After vacuum freeze drying, Xue's sea dragon peptide powder (labeled as sea dragon peptide powder) was obtained.

[0033] Example 1 Glucose-β-cyclodextrin treatment

[0034] Composite deodorization method to reduce the fishy smell of Xue's sea dragon peptide powder:

[0035] Take 100mL of 4wt% glucose aqueous solution, add 5g of prepared Xue's sea dragon peptide powder, and place in a water bath at 40°C for 3h; then add 1.5g of β-cyclodextrin (37.5% of the mass of glucose) to the system, disperse at 10000rpm for 1min, high-pressure homogenize at 80MPa for 3 times, take 20mL of the uniform solution, and dry with hot air at 40°C for 11h to obtain the deodorized Xue's sea dragon peptide powder.

[0036] Comparative Example 1 Drying Treatment

[0037] Take 100mL of water and add 5g of Xue's sea dragon peptide powder, place in a 40℃ water bath for 3h, disperse at a disperser speed of 10000rpm for 1min, homogenize at 80MPa high pressure for 3 times, take 20mL of the uniform solution, and dry with hot air at 40℃ for 11h to obtain the deodorized Xue's sea dragon peptide powder.

[0038] Comparative Example 2 Glucose Treatment

[0039] Take 100mL of 4wt% glucose aqueous solution, add 5g of Xue's Hailong peptide powder, place in a 40℃ water bath for 3h, disperse at a disperser speed of 10000rpm for 1min, homogenize at 80MPa for 3 times, take 20mL of uniform solution, and dry with hot air at 40℃ for 11h.

[0040] Comparative Example 3 β-cyclodextrin treatment

[0041] Take 100mL of water and add 5g of Xue's sea dragon peptide powder, place in a water bath at 40℃ for 3h, add 1.5g of β-cyclodextrin, disperse at a disperser speed of 10000rpm for 1min, homogenize at 80MPa high pressure for 3 times, take 20mL of the uniform solution, and dry with hot air at 40℃ for 11h to obtain the deodorized Xue's sea dragon peptide powder.

[0042] Effect verification example 1:

[0043] The Xue's sea dragon peptide powder treated in Example 1 and Comparative Examples 1-3 was placed in a sealed tube and sealed with a sealing film for 30 minutes. Eight sensory assessors aged between 20 and 26 who had been trained in food sensory evaluation courses were randomly selected to score the samples according to the fishy smell evaluation standard after sniffing, and each sample had a score between 1 and 5 points. The score indicates the severity of the fishy smell. The sensory assessor can retain one decimal place according to the scoring situation. After the statistics are completed, the total score of each sample is analyzed. The fishy smell sensory evaluation standard is shown in Table 1, and the evaluation results are shown in Table 2.

[0044] Table 1 Sensory evaluation standards

[0045]

[0046] Table 2 Sensory evaluation results

[0047]

[0048] Note: Different letters in the same column indicate significant differences, P<0.05.

[0049] Effect verification example 2

[0050] Electronic nose detection: Accurately weigh 1g of samples of Example 1 and Comparative Examples 1-3 respectively, place them in a 10mL beaker, seal them with plastic wrap, and let them stand at 25°C for 30min before testing. Measurement parameters: cleaning time 100s, zeroing time 5s, pre-injection time 10s, measurement time 80s, carrier gas flow rate 300mL·min -1 , chamber flow rate 400mL·min -1 , select 58s when the signal is stable as the signal acquisition time.

[0051] Figure 1 The radar graphs of the electronic nose sensor of the Hailong peptide powder after deodorization in different treatment groups of Example 1 and Comparative Examples 1-3. Among them, (a) is the radar graph of the electronic nose sensor of the Hailong peptide powder and the samples obtained from each treatment group, and (b) is the radar graph of the electronic nose sensor of the samples obtained from each treatment group.

[0052] like Figure 1 As shown in (a), compared with the raw material Hailong peptide powder, after being treated with glucose, β-cyclodextrin and glucose-β-cyclodextrin respectively to remove the fishy smell, the response values ​​of Hailong peptide powder in each sensor were reduced to varying degrees, among which the response values ​​of sensors W1W (inorganic sulfide), W2W (organic sulfide) and W5S (nitrogen oxides) were significantly reduced. In addition, compared with the simple drying treatment ( Figure 1 In (b), it was found that the electronic nose response values ​​of the three deodorization methods were further reduced not only in W1W (inorganic sulfides), W2W (organic sulfides) and W5S (nitrogen oxides), but also in W1S (methyls) and W2S (alcohols, aldehydes, ketones), indicating that the irritating odor was significantly reduced.

[0053] Effect verification example 3

[0054] Component detection using gas chromatography-mass spectrometry:

[0055] Extraction conditions: Take 0.25 g of sample, place it in a 20 mL extraction bottle, add a certain amount of internal standard (25 μg mL -1 2-octanol 10 μL), equivalent to 1 μg g -1 , sealed, placed in a 60℃ water bath, stirred at 300rpm for 20min, inserted an activated extraction needle for headspace extraction for 30min. Before use, the extraction needle was activated at 250℃ for 20min in the gas injection port.

[0056] Gas phase conditions: injection port temperature 250°C, gas chromatography-mass spectrometry interface temperature 250°C, carrier gas flow rate 1.2 mL min -1 , splitless injection.

[0057] Injection: The extraction needle was placed in the injection port for 20 minutes.

[0058] Heating program: Initial 40℃, hold for 5min, then increase by 5℃·min -1 Heating to 240℃ and holding for 0min; at 10℃·min -1 Under the same conditions, the temperature was raised to 250°C and maintained for 6 min.

[0059] Mass spectrometry conditions: ion source temperature 230°C, quadrupole temperature 150°C, EI ionization 70 eV, full scan 29-550 Da.

[0060] Figure 2 The changes in the amount of flavor substances in the haloperidol powder before and after deodorization treatment under different treatment methods in Example 1 of the present invention and Comparative Examples 1-3 are shown.

[0061] Figure 3 The distribution of the peak area (a) of the volatile components and the relative internal standard content (b) of each sample under different treatment methods in Example 1 of the present invention and Comparative Examples 1-3.

[0062] Using GC-MS technology, the changes in volatile compounds in Hailong peptide powder before and after deodorization were analyzed. In the raw Hailong peptide powder, glucose-β-cyclodextrin treatment, glucose treatment, β-cyclodextrin treatment, and drying treatment samples, 126, 88, 91, 93, and 94 volatile components were detected, respectively. Figure 2 ). By analyzing the peak area of ​​volatile components of the sample ( Figure 3 (a)) shows that in the volatile component detection of Hailong peptide powder, acids accounted for 24%, trimethylamine accounted for 23%, alcohols accounted for 22%, ketones accounted for 7%, aldehydes accounted for 4%, hydrocarbons, aromatics, ethers, esters accounted for 6%, 4%, 3%, 1% respectively, and other volatile substances accounted for 6%. Among them, acids, trimethylamine, and alcohols accounted for a total of 69%, which are the main components of the total volatile substances of Hailong peptide powder. Quantitative result analysis ( Figure 3 (b) also shows that the relative content of acids in Hailong peptide powder is the highest, followed by trimethylamine and alcohol, which are 271.7 μg·g -1 , 262.85μg·g -1 , 253.42μg·g -1 After the Hailong peptide powder was deodorized by three methods, the trimethylamine content was reduced to varying degrees compared with the raw materials, indicating that this method has a certain effect in removing the fishy smell. Among them, the types of volatile substances after glucose-β-cyclodextrin treatment were reduced the most ( Figure 2 ), the reduction of trimethylamine content was the largest, reaching 93% ( Figure 3 (b)).

[0063] In summary, through sensory evaluation, electronic nose, and GC-MS as evaluation basis, a comprehensive comparative analysis showed that the deodorization effect after glucose-β-cyclodextrin combined treatment was better, which could effectively reduce the relative content of fishy component trimethylamine and irritating component aldehydes, thereby improving the flavor of Xue's sea dragon peptide powder.

[0064] The embodiments described above are only descriptions of the preferred modes of the present invention, and are not intended to limit the scope of the present invention. Without departing from the design spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by ordinary technicians in this field should all fall within the protection scope determined by the claims of the present invention.

Claims

1. A composite deodorization method for reducing the fishy smell of Xue's sea dragon peptide powder, characterized in that: The following steps are involved: The Xue's halodendron peptide powder is added to the glucose aqueous solution, kept at 40-50° C. for 3-5 hours, and then cooled to room temperature; β-cyclodextrin is then added to the reaction system for dispersion and homogenization, and the Xue's halodendron peptide powder after deodorization is obtained after drying; The Xue's sea dragon peptide powder is prepared by the following steps: 10 g of Hesperus schrenkiana was crushed, 100 mL of double distilled water was added, the pH was adjusted to 8 using a 1 M sodium hydroxide solution, and then 100 mg of alkaline protease was added for enzymolysis at 50 ° C for 5 h. The obtained enzymolysis solution was inactivated by boiling water bath at 100 ° C for 10 min, centrifuged at 8000 r / min for 15 min, the supernatant was taken, dialyzed with an ultrafiltration membrane, and a fraction less than 3 kDa was taken. After vacuum freeze drying, the Hesperus schrenkiana peptide powder was obtained; The concentration of the glucose aqueous solution is 4wt%; The added amount of the β-cyclodextrin is 37.5% of the mass of glucose.

2. The composite deodorization method according to claim 1, characterized in that: The homogenization is high-pressure homogenization; the pressure of the high-pressure homogenization is 70-80MPa.

3. The composite deodorization method according to claim 1, characterized in that: The drying temperature is 40-50°C; the drying time is 8-12h.

4. The Xue's sea dragon peptide powder obtained by the composite deodorization method according to any one of claims 1 to 3.

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