A composition with plant growth regulating function and preparation method thereof

Abstract

The invention relates to a composition with a plant growth regulating function and a preparation method thereof, and belongs to the technical field of plant growth regulators. The invention uses cytokinin, auxin, brassinolide, zinc sulfate, a thickener, an antifreeze agent, and a self-made composite functional agent as raw materials to prepare a composition with a plant growth regulating function. The invention firstly mixes zinc sulfate and the antifreeze agent with deionized water and stirs to obtain a liquid A, and simultaneously dissolves the cytokinin, auxin, brassinolide, and the composite functional agent with an ethanol aqueous solution and stirs to mix at a high speed to obtain a liquid B, and finally compounding the thickener, the liquid A, and the liquid B according to a required ratio and ultrasonically dispersing to obtain a composition with a plant growth regulating function. The composition of the invention has uniform and controllable components, is uniform and stable, can be adapted to different types of tobacco, and the prepared composition can not only promote the growth of tobacco leaves, but also have a good inhibitory effect on a variety of pathogens and viruses.

Description

Technical Field

[0001] The present invention belongs to the technical field of plant growth regulators, and in particular, relates to a composition with plant growth regulating function and a preparation method thereof. Background Art

[0002] Plant growth regulators are chemical substances that are artificially synthesized (or extracted from microorganisms) based on the structure, function and action principle of plant hormones and have a regulating effect on plant growth and development. According to the similarity with the action of plant hormones, they can be divided into six categories: auxins, gibberellins, cytokinins, abscisic acid, ethylene and brassinolide. Plant growth regulators regulate the growth and development of plants by affecting the synthesis, metabolism and signal transduction of endogenous hormones in plants. They regulate the division and elongation of plant cells, differentiation of tissues and organs, flowering and fruiting, maturity and aging, dormancy and germination, etc., separately or in collaboration, so as to achieve the desired effect. Plant growth regulators have been widely used in agricultural production, including food crops, vegetables and fruits, fruit tree gardening and other fields. They can improve the quality of crops, enhance the stress resistance of crops, stabilize and increase yields, etc. However, since most plant growth regulators have poor solubility in water, different types of plant growth regulators cannot be used in combination with other agricultural additives.

[0003] Based on this, the present invention provides a composition with plant growth regulating function and a preparation method thereof, so as to prepare a multifunctional plant growth regulator. Summary of the invention

[0004] The object of the present invention is to provide a composition with plant growth regulating function and a preparation method thereof, so as to solve the problems mentioned in the above background technology.

[0005] The purpose of the present invention can be achieved through the following technical solutions:

[0006] A composition with plant growth regulating function, comprising the following raw materials in parts by weight: 0.1-0.4 parts of cytokinin, 13-15 parts of auxin, 3.5-4.1 parts of brassinolide, 1.7-2.5 parts of zinc sulfate, 1.4-1.8 parts of thickener, 24-60 parts of antifreeze agent, and 2.7-4.5 parts of composite functional agent;

[0007] A method for preparing a composition having a plant growth regulating function comprises the following steps:

[0008] Step 1: weigh the raw materials according to the mass fractions: 0.1-0.4 parts of cytokinin, 13-15 parts of auxin, 3.5-4.1 parts of brassinolide, 1.7-2.5 parts of zinc sulfate, 1.4-1.8 parts of thickener, 24-60 parts of antifreeze agent, and 2.7-4.5 parts of composite functional agent;

[0009] Step 2: Dissolve zinc sulfate and antifreeze agent in deionized water, and stir to mix to obtain liquid A;

[0010] Step 3: Dissolve the cytokinin, auxin, brassinolide and composite functional agent in ethanol aqueous solution, and mix them with high-speed stirring to obtain liquid B;

[0011] Step 4: After compounding the thickener, liquid A and liquid B, ultrasonically disperse them to obtain a composition with plant growth regulating function.

[0012] Furthermore, the cytokinin is 6-benzylaminopurine.

[0013] Furthermore, the auxin is one of indoleacetic acid, indolebutyric acid and naphthylacetic acid.

[0014] Furthermore, the thickener is one of bentonite and xanthan gum.

[0015] Furthermore, the antifreeze agent is one of ethylene glycol, glycerol and propylene glycol.

[0016] Furthermore, in the second step, the stirring speed condition is 100-200 r / min, and the stirring speed condition is 10-15 min.

[0017] Furthermore, in the third step, the rotation speed condition of high-speed stirring is 1500-2000r / min, and the rotation speed condition of high-speed stirring is 20-30min.

[0018] Furthermore, the ultrasonic dispersion time in the fourth step is 30-60 min.

[0019] Furthermore, the composite functional agent is prepared by the following steps:

[0020] Step 1: Mix 2-vinylpyridine and diethyl phosphite in a three-necked flask. Install a condenser and a thermometer, start magnetic stirring, and react at 50-70°C for 2-3 hours. After the reaction is completed, rotary evaporate to obtain intermediate 1;

[0021] Step 2: Mix the intermediate 1, chloroacetic acid and acetonitrile in a three-necked flask, install a condenser and a thermometer, start magnetic stirring, and react at a temperature of 40-60°C for 18-24h. After the reaction is completed, rotary evaporation is performed to obtain the intermediate 2;

[0022] Step 3: Dissolve the intermediate II, chitosan oligosaccharide, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride, and N-hydroxysuccinimide in deionized water, turn on magnetic stirring, adjust the pH to 4-5 with dilute hydrochloric acid, and then react with magnetic stirring at room temperature for 48 hours. After the reaction is completed, add a large amount of ethanol to a three-necked flask for precipitation, and then vacuum filter. The obtained solid is washed with ethanol and dried to obtain the composite functional agent;

[0023] Furthermore, the amount ratio of 2-vinylpyridine and diethyl phosphite used in step 1 is 0.1 mol: 0.1-0.15 mol.

[0024] Furthermore, the usage ratio of the intermediate 1, chloroacetic acid and acetonitrile used in step 2 is 0.08 mol: 0.08-0.1 mol: 30-40 mL.

[0025] Furthermore, the amount ratio of the intermediate used in step 3, chitosan oligosaccharide, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride, N-hydroxysuccinimide, and deionized water is 1.3-1.5g: 3g: 1.6g-2.1g: 0.7-0.9g: 50-60g.

[0026] Beneficial effects of the present invention:

[0027] 1) The present invention uses cytokinin, auxin, brassinolide, zinc sulfate, thickener, antifreeze agent, and self-made composite functional agent as raw materials to prepare a composition with plant growth regulating function; the present invention firstly dissolves zinc sulfate and antifreeze agent in deionized water and stirs to obtain liquid A, and at the same time dissolves cytokinin, auxin, brassinolide, and composite functional agent in ethanol aqueous solution and stirs to obtain liquid B at high speed, and finally the thickener, liquid A, and liquid B are compounded in a required proportion and ultrasonically dispersed to obtain a composition with plant growth regulating function. The composition with plant growth regulating function prepared by the preparation method of the present invention has good promoting effect on tobacco growth, and the components are controllable, and can be adapted to different types of tobacco.

[0028] 2) The present invention uses 2-vinylpyridine and diethyl phosphite as raw materials, and obtains intermediate 1 by a nucleophilic addition reaction on the double bond of 2-vinylpyridine. Then, using intermediate 1 as a raw material, intermediate 1 and chloroacetic acid undergo a quaternary ammonium salt reaction to obtain intermediate 2. Finally, the carboxyl group of intermediate 2 and the amino group of chitosan oligosaccharide undergo an amidation reaction under the catalytic action of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride and N-hydroxysuccinimide to obtain a composite functional agent. The composite functional agent of the present invention has a hydrophilic cationic quaternary ammonium salt structure and a lipophilic organic structure, which can reduce the surface tension of the solution interface, promote the dispersion of the active ingredients in the composition in the system, improve the stability of the composition system, and improve the solubility of the organic matter in the active ingredients in the solvent. In addition, the composite functional agent of the present invention also has a pyridyl quaternary ammonium salt structure and an aminophosphoric acid ester structure, which can play a good inhibitory effect on various pathogens and plant viruses, and can synergistically exert an inhibitory effect on tobacco mosaic virus with brassinolide in the composition components.

[0029] 3) The composition with plant growth regulating function prepared by the preparation method of the present invention has uniform and controllable components, is uniform and stable, and can be adapted to different types of tobacco. The prepared composition can not only promote the growth of tobacco leaves, but also have a good inhibitory effect on a variety of pathogens and viruses. DETAILED DESCRIPTION

[0030] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0031] Example 1

[0032] A composite functional agent is prepared by the following steps:

[0033] Step 1: Mix 0.1 mol 2-vinylpyridine and 0.1 mol diethyl phosphite in a three-necked flask. Install a condenser and a thermometer, turn on the magnetic stirring, and react at 50°C for 2 hours. After the reaction is completed, rotary evaporate to obtain intermediate 1;

[0034] Step 2: Mix 0.08 mol of intermediate 1, 0.08 mol of chloroacetic acid and 30 mL of acetonitrile in a three-necked flask, install a condenser and a thermometer, start magnetic stirring, and react at 40°C for 18 hours. After the reaction is completed, rotary evaporate to obtain intermediate 2;

[0035] Step 3: Dissolve 1.3 g of intermediate di, 3 g of chitosan oligosaccharide, 1.6 g of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride, and 0.7 g of N-hydroxysuccinimide in 50 g of deionized water, turn on magnetic stirring, adjust the pH to 4 with dilute hydrochloric acid, and then react with magnetic stirring at room temperature for 48 hours. After the reaction is completed, add a large amount of ethanol to a three-necked flask for precipitation, and then vacuum filter. The obtained solid is washed with ethanol and dried to obtain the composite functional agent.

[0036] Example 2

[0037] A composite functional agent is prepared by the following steps:

[0038] Step 1: Mix 0.1 mol 2-vinylpyridine and 0.13 mol diethyl phosphite in a three-necked flask. Install a condenser and a thermometer, turn on the magnetic stirring, and react at 60°C for 2.5 hours. After the reaction is completed, rotary evaporate to obtain intermediate 1;

[0039] Step 2: Mix 0.08 mol of intermediate 1, 0.09 mol of chloroacetic acid and 35 mL of acetonitrile in a three-necked flask, install a condenser and a thermometer, start magnetic stirring, and react at 50°C for 21 hours. After the reaction is completed, rotary evaporation is performed to obtain intermediate 2;

[0040] Step 3: Dissolve 1.4 g of intermediate di, 3 g of chitosan oligosaccharide, 1.8 g of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride, and 0.8 g of N-hydroxysuccinimide in 55 g of deionized water, turn on magnetic stirring, adjust the pH to 4.5 with dilute hydrochloric acid, and then react with magnetic stirring at room temperature for 48 hours. After the reaction is completed, add a large amount of ethanol to a three-necked flask for precipitation, and then vacuum filter. The obtained solid is washed with ethanol and dried to obtain the composite functional agent.

[0041] Example 3

[0042] A composite functional agent is prepared by the following steps:

[0043] Step 1: Mix 0.1 mol 2-vinylpyridine and 0.15 mol diethyl phosphite in a three-necked flask. Install a condenser and a thermometer, start magnetic stirring, and react at 70°C for 3 hours. After the reaction is completed, rotary evaporate to obtain intermediate 1;

[0044] Step 2: Mix 0.08 mol of intermediate 1, 0.1 mol of chloroacetic acid and 40 mL of acetonitrile in a three-necked flask, install a condenser and a thermometer, turn on the magnetic stirring, and react at 60°C for 24 hours. After the reaction is completed, rotary evaporate to obtain intermediate 2;

[0045] Step 3: Dissolve 1.5 g of intermediate di, 3 g of chitosan oligosaccharide, 2.1 g of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride, and 0.9 g of N-hydroxysuccinimide in 60 g of deionized water, turn on magnetic stirring, adjust the pH to 5 with dilute hydrochloric acid, and then react with magnetic stirring at room temperature for 48 hours. After the reaction is completed, add a large amount of ethanol to a three-necked flask for precipitation, and then vacuum filter. The obtained solid is washed with ethanol and dried to obtain the composite functional agent.

[0046] Comparative Example 1

[0047] This comparative example is the mosaic virus disinfectant sold by Zibo Meishou Agricultural Technology Co., Ltd.

[0048] The composite functional agent obtained in Examples 1-3 and the commercially available mosaic virus killer in Comparative Example 1 were prepared into a sterilizing liquid and sprayed on healthy tobacco plants. The whole plant was inoculated with tobacco mosaic virus 24h and 48h after the application of the drug, and the number of spots on the plant was recorded. The inhibition rate of the composite functional agent of the present invention and the commercially available mosaic virus killer on tobacco mosaic virus was compared. The test results are shown in Table 1:

[0049] Table 1

[0050]

[0051] It can be seen from Table 1 that the number of spots on the plants sprayed with the composite functional agent of the present invention is much smaller than the number of spots on the plants sprayed with the commercially available mosaic virus disinfectant, indicating that the composite functional agent of the present invention has a much better inhibitory effect on tobacco mosaic virus than the commercially available mosaic virus disinfectant, and the composition with plant growth regulating function of the present invention can effectively inhibit plant viruses.

[0052] Example 4

[0053] A composition with a plant growth regulating function, comprising the following raw materials in parts by weight: 0.1 parts of 6-benzylaminopurine, 13 parts of indoleacetic acid, 3.5 parts of brassinolide, 1.7 parts of zinc sulfate, 1.4 parts of bentonite, 24 parts of ethylene glycol, and 2.7 parts of the composite functional agent obtained in Example 1;

[0054] A method for preparing a composition having a plant growth regulating function comprises the following steps:

[0055] Step 1: weigh the raw materials according to their mass fractions: 0.1 parts of 6-benzylaminopurine, 13 parts of indoleacetic acid, 3.5 parts of brassinolide, 1.7 parts of zinc sulfate, 1.4 parts of bentonite, 24 parts of ethylene glycol, and 2.7 parts of the composite functional agent obtained in Example 1;

[0056] Step 2: Dissolve zinc sulfate and ethylene glycol in deionized water, and stir at a speed of 100 r / min for 10 min to obtain liquid A;

[0057] Step 3: Dissolve 6-benzylaminopurine, indoleacetic acid, brassinolide, and the composite functional agent obtained in Example 1 in an ethanol aqueous solution, and stir at a high speed of 1500 r / min for 20 min to obtain liquid B;

[0058] Step 4: After compounding bentonite, liquid A and liquid B, ultrasonically disperse them for 30 minutes to obtain a composition with plant growth regulating function.

[0059] Example 5

[0060] A composition with a plant growth regulating function, comprising the following raw materials in parts by weight: 0.25 parts of 6-benzylaminopurine, 14 parts of indolebutyric acid, 3.8 parts of brassinolide, 2.1 parts of zinc sulfate, 1.6 parts of bentonite, 42 parts of glycerol, and 3.6 parts of the composite functional agent obtained in Example 2;

[0061] A method for preparing a composition having a plant growth regulating function comprises the following steps:

[0062] Step 1: weigh the raw materials according to their mass fractions: 0.25 parts of 6-benzylaminopurine, 14 parts of indolebutyric acid, 3.8 parts of brassinolide, 2.1 parts of zinc sulfate, 1.6 parts of bentonite, 42 parts of glycerol, and 3.6 parts of the composite functional agent obtained in Example 2;

[0063] Step 2: Dissolve zinc sulfate and glycerol in deionized water, and stir at a speed of 150 r / min for 12 min to obtain liquid A;

[0064] Step 3: Dissolve 6-benzylaminopurine, indolebutyric acid, brassinolide, and the composite functional agent obtained in Example 2 in an ethanol aqueous solution, and stir at a high speed of 1750 r / min for 25 min to obtain liquid B;

[0065] Step 4: After compounding bentonite, liquid A and liquid B, ultrasonically disperse them for 45 minutes to obtain a composition with plant growth regulating function.

[0066] Example 6

[0067] A composition with a plant growth regulating function, comprising the following raw materials in parts by weight: 0.4 parts of 6-benzylaminopurine, 15 parts of naphthaleneacetic acid, 4.1 parts of brassinolide, 2.5 parts of zinc sulfate, 1.8 parts of xanthan gum, 60 parts of propylene glycol, and 4.5 parts of the composite functional agent obtained in Example 3;

[0068] A method for preparing a composition having a plant growth regulating function comprises the following steps:

[0069] Step 1: weigh the raw materials according to their mass fractions: 0.4 parts of 6-benzylaminopurine, 15 parts of naphthaleneacetic acid, 4.1 parts of brassinolide, 2.5 parts of zinc sulfate, 1.8 parts of xanthan gum, 60 parts of propylene glycol, and 4.5 parts of the composite functional agent obtained in Example 3;

[0070] Step 2: Dissolve zinc sulfate and propylene glycol in deionized water, and stir at a speed of 200 r / min for 15 min to obtain liquid A;

[0071] Step 3: Dissolve 6-benzylaminopurine, naphthylacetic acid, brassinolide, and the composite functional agent obtained in Example 3 in an ethanol aqueous solution, and stir at a high speed of 2000 r / min for 30 min to obtain liquid B;

[0072] Step 4: After compounding bentonite, liquid A and liquid B, ultrasonically disperse them for 60 minutes to obtain a composition with plant growth regulating function.

[0073] Comparative Example 2

[0074] A composition with a plant growth regulating function, comprising the following raw materials in parts by weight: 0.4 parts of 6-benzylaminopurine, 15 parts of naphthaleneacetic acid, 4.1 parts of brassinolide, 2.5 parts of zinc sulfate, 1.8 parts of xanthan gum, and 60 parts of propylene glycol;

[0075] A method for preparing a composition having a plant growth regulating function comprises the following steps:

[0076] Step 1: weigh the raw materials according to their mass fractions: 0.4 parts of 6-benzylaminopurine, 15 parts of naphthaleneacetic acid, 4.1 parts of brassinolide, 2.5 parts of zinc sulfate, 1.8 parts of xanthan gum, and 60 parts of propylene glycol;

[0077] Step 2: Dissolve zinc sulfate and propylene glycol in deionized water, and stir at a speed of 200 r / min for 15 min to obtain liquid A;

[0078] Step 3: Dissolve 6-benzylaminopurine, naphthylacetic acid and brassinolide in ethanol aqueous solution, and stir at a high speed of 2000 r / min for 30 min to obtain solution B;

[0079] Step 4: After compounding bentonite, liquid A and liquid B, ultrasonically disperse them for 60 minutes to obtain a composition with plant growth regulating function.

[0080] Comparative Example 3

[0081] This comparative example is a plant growth promoter sold by Jinan Yeqing Biotechnology Co., Ltd.

[0082] The composition with plant growth regulating function obtained in Examples 4-6 and Comparative Example 2 and the plant growth promoter in Comparative Example 3 were tested respectively, and applied to different tobacco test fields in the same environment, and the average height and average leaf length of the mature plants were recorded. The record structure is shown in Table 2:

[0083] Table 2

[0084]

[0085] It can be seen from Table 2 that the plant height and leaf length of the tobacco test field where the composition with plant growth regulating function obtained in Examples 4-6 of the present invention was applied were better than the plant height and leaf length of the tobacco test field where the commercially available plant growth promoter was applied, while the plant height of the tobacco test field where the composition with plant growth regulating function in Comparative Example 2 was applied was similar to that of the test field where the commercial promoter was applied, indicating that the addition of the composite functional agent of the present invention can effectively promote the effectiveness of the active ingredients in the composition, and the composition with plant growth regulating function of the present invention has a good promoting effect on plant growth.

[0086] The above is a detailed introduction to a composition with plant growth regulating function and a preparation method thereof provided by the present invention. Specific examples are used herein to illustrate the principles and implementation methods of the present invention. The description of the above embodiments is only used to help understand the method of the present invention and its core ideas, including the best mode, and also enables any technician in the field to practice the present invention, including manufacturing and using any device or system, and implementing any combination method. It should be pointed out that for ordinary technicians in this technical field, the present invention can also be improved and modified without departing from the principle of the present invention. In particular, as long as there is no structural conflict, the various features in the embodiments disclosed in the present invention can be used in combination with each other in any way. The fact that these combinations are not exhaustively described in this specification is only for the purpose of omitting space and saving resources. Therefore, the present invention is not limited to the specific embodiments disclosed herein, but includes all technical solutions falling within the scope of the claims.

Claims

1. A composition having a plant growth regulating function, characterized in that: The invention comprises the following raw materials in parts by weight: 0.1-0.4 parts of cytokinin, 13-15 parts of auxin, 3.5-4.1 parts of brassinolide, 1.7-2.5 parts of zinc sulfate, 1.4-1.8 parts of thickener, 24-60 parts of antifreeze agent, and 2.7-4.5 parts of composite functional agent; Wherein, the composite functional agent is prepared by the following steps: Step 1: Mix 2-vinylpyridine and diethyl phosphite in a container; After stirring evenly, react at 50-70°C for 2-3h to obtain intermediate 1; Step 2: Mix intermediate 1, chloroacetic acid and acetonitrile in a container, stir evenly, and react at 40-60° C. for 18-24 hours to obtain intermediate 2; Step 3: Dissolve the intermediate 2, chitosan oligosaccharide, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride, and N-hydroxysuccinimide in deionized water, stir evenly, adjust the pH to 4-5 with dilute hydrochloric acid, and then react at room temperature for 48 hours to obtain the composite functional agent.

2. A composition having a plant growth regulating function according to claim 1, characterized in that: The amount ratio of 2-vinylpyridine and diethyl phosphite used in step 1 is 0.1 mol: 0.1-0.15 mol.

3. A composition having a plant growth regulating function according to claim 1, characterized in that: The amount ratio of intermediate 1, chloroacetic acid and acetonitrile used in step 2 is 0.08 mol: 0.08-0.1 mol: 30-40 mL.

4. A composition having a plant growth regulating function according to claim 1, characterized in that: The amount ratio of intermediate 2, chitosan oligosaccharide, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride, N-hydroxysuccinimide, and deionized water used in step 3 is 1.3-1.5g: 3g: 1.6g-2.1g: 0.7-0.9g: 50-60g.

5. A method for preparing a composition having a plant growth regulating function as claimed in any one of claims 1 to 4, characterized in that: The following steps are involved: Step 1: weigh the raw materials according to the mass fractions: 0.1-0.4 parts of cytokinin, 13-15 parts of auxin, 3.5-4.1 parts of brassinolide, 1.7-2.5 parts of zinc sulfate, 1.4-1.8 parts of thickener, 24-60 parts of antifreeze agent, and 2.7-4.5 parts of composite functional agent; Step 2: Dissolve zinc sulfate and antifreeze agent in deionized water, and stir to mix to obtain liquid A; Step 3: Dissolve the cytokinin, auxin, brassinolide and composite functional agent in ethanol aqueous solution, and mix them with high-speed stirring to obtain liquid B; Step 4: After compounding the thickener, liquid A and liquid B, ultrasonically disperse them to obtain a composition with plant growth regulating function.

6. The method for preparing a composition having a plant growth regulating function according to claim 5, characterized in that: The cytokinin is 6-benzyladenine, and the auxin is one of indoleacetic acid, indolebutyric acid, and naphthylacetic acid.

7. The method for preparing a composition having a plant growth regulating function according to claim 5, characterized in that: The thickener is one of bentonite and xanthan gum, and the antifreeze agent is one of ethylene glycol, glycerol and propylene glycol.

8. The method for preparing a composition having a plant growth regulating function according to claim 5, characterized in that: In the second step, the stirring speed condition is 100-200r / min, and the stirring time condition is 10-15min.

9. The method for preparing a composition having a plant growth regulating function according to claim 5, characterized in that: In the third step, the speed condition of high-speed stirring is 1500-2000r / min, and the time condition of high-speed stirring is 20-30min.

10. The method for preparing a composition having a plant growth regulating function according to claim 5, characterized in that: The time condition of ultrasonic dispersion in the fourth step is 30-60min.

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