A pesticide composition for preventing and controlling white grubs

Abstract

The present invention belongs to the field of pesticide technology, and specifically relates to a pesticide composition for controlling white grubs. The insecticidal composition is formed by compounding dichlorothiapyrim and rotenone, or by compounding dichlorothiapyrim and bifenthrin. The compounded insecticidal composition of the present invention exhibits a synergistic effect on second-instar white grub larvae, improving the control efficacy against white grubs, reducing pesticide usage and pesticide residues, and achieving high efficiency, low residue, and environmentally friendly performance. This is of great significance for the development of highly effective white grub control agents.

Description

Technical Field

[0001] The invention belongs to the technical field of pesticides, and particularly relates to a pesticide composition for preventing and controlling white grubs. Background Art

[0002] White grubs, the general term for the larvae of the Scarabaeidae family in the order Coleoptera, represent the most diverse, widespread, and devastating group of underground pests. Larvae primarily feed on the underground rhizomes and pods of various crops, causing them to wilt and die. Adult scarab beetles feed on the leaves of crops, fruit trees, and forest trees, causing bud and leaf damage and severely impacting the quality and yield of agricultural products. With the restrictions and bans on the use of some highly toxic and high-residue pesticides, many current pesticides are ineffective against white grubs. Therefore, the development of effective pesticides for white grub control is crucial.

[0003] Dichlorothiopyrimidine (dicloromezotiaz) is a new type of mesoionic pyrimidone insecticide developed by DuPont (now Corteva) in the United States. Its CAS registration number is 1263629-39-5 and its structural formula is as follows:

[0004]

[0005] Unlike neonicotinoid insecticides, dichlorothiapyrimidine (DCT) acts on nicotinic acetylcholine receptors, exerting its insecticidal activity by blocking neurotransmission in target pests. This is known as a nicotinic acetylcholine receptor inhibitor. DCT has a novel chemical structure and mechanism of action, is highly effective, has low toxicity, and is environmentally friendly. It has high activity against Plutella xylostella, armyworm, and peach aphid, and is highly effective against Lepidoptera and Hemiptera pests at low doses.

[0006] Although dichlorothiazide has the above advantages, as a chemical insecticide, if used alone for a long time, it will inevitably cause the development and accumulation of pest resistance, like other chemical insecticides, leading to a decrease in its control effectiveness. Therefore, it is necessary to screen for compound agents of dichlorothiazide.

[0007] The inventors screened and compounded dichlorothiapyrim with existing insecticidal active ingredients and found that when dichlorothiapyrim is compounded with rotenone or bifenthrin, it exhibits a synergistic effect on white grubs, which can improve the control effect on white grubs and can be used to develop highly effective agents for controlling underground pests white grubs.

[0008] The information disclosed in this background technology section is only intended to enhance understanding of the overall background of the invention and should not be regarded as an admission or any form of suggestion that the information constitutes the prior art already known to a person skilled in the art. Summary of the Invention

[0009] The present invention aims to provide a pesticide composition for controlling white grubs, which can reduce the risk of resistance to dichlorothiazide and protect dichlorothiazide to a certain extent; at the same time, it can improve the control effect on pests.

[0010] To achieve the above object, the present invention provides the following technical solutions:

[0011] Disclosed is an insecticide composition, which is compounded by dichlorothiapyrim and rotenone, or by dichlorothiapyrim and bifenthrin.

[0012] More specifically, the mass ratio of dichlorothiazide to rotenone is 1-18:24-1.

[0013] More specifically, the mass ratio of diclofenac to bifenthrin is 1-40:15-1.

[0014] The present invention also provides the use of the insecticide composition in preventing and controlling underground pests of crops or in preparing a pesticide for preventing and controlling underground pests of crops.

[0015] More specifically, the underground crop pests include white grubs.

[0016] Compared with the prior art, the present invention has the following beneficial effects:

[0017] (1) The insecticide composition of the present invention is a binary compound of dichlorothiapyrim and rotenone or bifenthrin, which contains two active ingredients. Compared with the use of dichlorothiapyrim alone, it can reduce the risk of pest resistance, delay the generation and development of pest resistance, and protect dichlorothiapyrim to a certain extent.

[0018] (2) The compound combination of the insecticide composition of the present invention exhibits a combined synergistic effect on the second-instar larvae of white grubs, which can improve the control effect on white grubs, reduce the amount of pesticides used and pesticide residues, and achieve high efficiency, low residue, and green environmental protection of the agent, which is of great significance for the development of high-efficiency agents for controlling white grubs. DETAILED DESCRIPTION

[0019] The technical solution of the present invention is described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them.

[0020] 1. Materials and Methods

[0021] 1.1 Test pests

[0022] A. corydalis was collected from peanut fields and reared in an artificial insectary on fresh, clean elm leaves. Rearing conditions included a temperature of 25 ± 1°C, a humidity of 65 ± 5%, and a photoperiod of 16 L / 8 days. Eggs of the same age were selected for hatching, and the hatched larvae were fed potato tubers. Second-instar larvae of uniform size were selected as test pests.

[0023] 1.2 Test agents

[0024] 95% dichlorothiazide technical (Hubei Guoyun Furui Technology Co., Ltd.), 94% rotenone technical (extracted from the roots of rotenone), 97% bifenthrin technical (Jiangsu Yangnong Chemical Co., Ltd.).

[0025] 1.3 Test method: (Refer to "NY / T 1154.6-2006 Guidelines for Indoor Bioassay Tests of Pesticides - Insecticides Part 6: Insecticide Activity Test - Insect Immersion Method").

[0026] Prepare the drug solution: Dissolve the original drug in dimethyl sulfoxide and then dilute with 0.05% Triton X-100 aqueous solution to prepare a single-dose stock solution. Then, set up multiple groups of proportions and dilute each single dose and each group of mixed drugs in equal proportions with 0.05% Triton X-100 aqueous solution to 5 mass concentration gradients, with 50 mL of drug solution for each mass concentration set aside.

[0027] For liquid treatment, immerse the test insects in the liquid for 20 seconds, remove them, absorb the excess liquid with absorbent paper, and place them in a finger-shaped tube containing potato tubers of uniform size. Seal the tube with a double layer of gauze. Each treatment was replicated four times, with 20 insects immersed in each replicate. A blank control was also used, using a 0.05% Triton X-100 aqueous solution.

[0028] After treatment, the insects were transferred to a room with a temperature of 25 ± 1°C and a humidity of 65 ± 5% in the absence of light. After 3 days, the insects were examined for mortality. Insects that did not move when lightly touched with a brush were considered dead.

[0029] 2. Data Processing

[0030] The total number of insects and the number of dead insects in each treatment were investigated, and the corrected mortality rate of each treatment was calculated. The DPS software was used to perform regression analysis on the logarithmic value of the concentration of each treatment agent and the corrected mortality probability value to calculate the LC value of each treatment agent. 50 .

[0031]

[0032] In the above formula: P is the mortality rate, in %; K is the number of dead insects; N is the total number of insects treated.

[0033]

[0034] In the above formula: P1--corrected mortality rate, unit is %; P t --Treatment mortality, unit is %; P0--blank control mortality, unit is %.

[0035] The co-toxicity coefficient (CTC value) of the mixture was calculated using the Sun Yunpei method, and the joint effect was divided according to the following criteria: a CTC ≥ 120 indicated a synergistic effect; a CTC ≤ 80 indicated an antagonistic effect; and a CTC < 80 < 120 indicated an additive effect.

[0036] 3. Results and Analysis

[0037] The results are shown in Table 1-2.

[0038] Table 1 The combined effects of dichlorothiazide and rotenone on the second-instar larvae of white grubs

[0039]

[0040]

[0041] As can be seen from Table 1, within the mass ratio of 1-18:24-1, the co-toxicity coefficient of the combination of dichlorothiapyrim and rotenone in killing the second-instar larvae of white grubs is greater than 120, that is, the combined action type of the combination of dichlorothiapyrim and rotenone in killing white grubs is a synergistic effect, which can improve the control effect on white grubs.

[0042] Table 2 The combined effect of dichlorothiazide and bifenthrin on the second-instar larvae of white grubs

[0043] Drug name and ratio <![CDATA[LC 50 (mg / L)]]> ATI TTI CTC dichlorothiazide 2.847 100.000 -- -- Bifenthrin 5.159 55.185 -- -- Diclofenac 1: Bifenthrin 15 4.054 70.227 57.986 121.110 Diclofenac 1: Bifenthrin 7 1.271 223.997 60.787 368.495 Diclofenac 1: Bifenthrin 3 3.168 89.867 66.389 135.365 Diclofenac 1: Bifenthrin 1 2.977 95.633 77.593 123.250 Dichlorothiazide 3: Bifenthrin 1 1.495 190.435 88.796 214.463 Dichlorothiazide 7: Bifenthrin 1 0.637 446.939 94.398 473.461 Diclofenac 15: Bifenthrin 1 0.928 306.789 97.199 315.629 Diclofenac 30: Bifenthrin 1 1.873 152.002 98.554 154.232 Diclofenac 40: Bifenthrin 1 2.272 125.308 98.907 126.693

[0044] As can be seen from Table 2, within the mass ratio of 1-40:15-1, the co-toxicity coefficient of the combination of dichlorothiapyrim and bifenthrin in killing the second-instar larvae of white grubs is greater than 120, that is, the combined action type of the combination of dichlorothiapyrim and bifenthrin in killing white grubs is a synergistic effect, which can improve the control effect on white grubs.

[0045] In summary, the compound combination of the insecticidal composition of the present invention exhibits a combined synergistic effect on the second-instar larvae of white grubs, can improve the control effect on white grubs, reduce the amount of pesticides used and pesticide residues, achieve high efficiency, low residue, and green environmental protection of the agent, which is of great significance for the development of high-efficiency agents for controlling white grubs.

Claims

1. An insecticidal composition, characterized in that The insecticide composition is prepared by compounding dichlorothiazide and rotenone, and the compounding mass ratio of the dichlorothiazide to rotenone is 1-18:24-1.

2. Use of the insecticide composition according to claim 1 in controlling underground pests of crops or in preparing a pesticide for controlling underground pests of crops.

3. The use according to claim 2, characterized in that The underground pests of crops include white grubs.

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