Novel biological organic water-soluble fertilizer and application thereof

Abstract

The invention discloses a novel biological organic water-soluble fertilizer and application thereof. The novel biological organic water-soluble fertilizer is prepared from the following raw materials: a starchy raw material fermentation concentrated solution, an inorganic fertilizer and serratia nematophila KWNj-P. The total nutrient content of the novel biological organic water-soluble fertilizer is 8-12%, the organic matter content is 20-22%, the effective viable count is 5 * 10 < 7 >-5 * 10 < 8 > CFU / mL, and the pH value is 5.5-6.5. The raw materials of the novel biological organic water-soluble fertilizer complement each other, and the novel biological organic water-soluble fertilizer contains a large number of nutrient elements and organic matters beneficial to plant growth, and is compounded with a microbial agent to form a comprehensive organic-inorganic total-factor biological fertilizer to keep nutrient balance. The novel biological organic water-soluble fertilizer disclosed by the invention can be dissolved in water for integrated application of water and fertilizer, can also be directly applied, is suitable for diversified modern agricultural production modes, and can promote crop growth, improve crop yield and improve crop disease resistance.

Description

Technical Field

[0001] The invention relates to the technical field of fertilizers, in particular to a novel biological organic water-soluble fertilizer and application thereof. Background Art

[0002] While chemical fertilizers have significantly improved agricultural production efficiency and crop yields, their excessive use has not only exacerbated problems such as soil compaction, acidification and reduced fertility, but has also had adverse effects on water quality, atmospheric environment and the quality of agricultural products, posing a serious threat to the sustainable development of my country's agriculture. With the continuous deepening of agricultural intensification, the problem of soil-borne diseases in China is becoming increasingly serious. Faced with multiple challenges such as the deterioration of soil, water quality, atmospheric environment and the decline in the quality of agricultural products caused by excessive use of chemical fertilizers, in order to promote the sustainable development of agriculture and meet the needs of water-fertilizer integration, the research and development of new liquid fertilizers has become a research hotspot in the field of agricultural resources and environment and a technical difficulty that needs to be overcome urgently.

[0003] Microbial fertilizers are a type of living microbial products that use microbial life activities and their metabolites to produce specific fertilizer effects on crops. They are indispensable products for the development of green and sustainable agriculture in my country, and have good application value and economic and social benefits. At present, microbial fertilizer products mainly include agricultural microbial fertilizers, compound microbial fertilizers, and bio-organic fertilizers. At present, most of the research is on inorganic liquid fertilizers, liquid fertilizers with microbial agents and inorganic nutrients, or single organic liquid fertilizers; research on bio-organic fertilizers is also focused on solid types, and there is little research on new bio-organic water-soluble fertilizers. As a new type of fertilizer, the new bio-organic water-soluble fertilizer not only has organic and inorganic nutrients and functional microorganisms, but is also easy to apply, saves time and effort, and has flexible formulas. It has better application effects in the agricultural planting process and is more suitable for the increasingly widely used water-fertilizer integration technology and equipment.

[0004] Therefore, in view of the long-term, large-scale and unreasonable application of chemical fertilizers, the slow effect of organic fertilizers, the serious shortage of application area and dosage, the low efficiency of biological fertilizers, the small application scale, the lack of regional targeting, and the difficulty of market promotion, as well as the fact that solid biological organic fertilizers are not suitable for water-fertilizer integrated equipment and most compound microbial liquid fertilizers do not have soluble organic matter, there is an urgent need to develop a new type of fertilizer that is both environmentally friendly and can promote crop growth to adapt to modern agricultural production methods. Summary of the invention

[0005] In view of the shortcomings of the prior art, the present invention provides a novel biological organic water-soluble fertilizer and its application. The novel biological organic water-soluble fertilizer of the present invention integrates the quick-acting property of inorganic fertilizer, the long-acting property of organic fertilizer and the synergistic property of biological fertilizer, and is suitable for diversified modern agricultural production methods.

[0006] To achieve the above object, the present invention provides the following solutions:

[0007] A novel biological organic water-soluble fertilizer, the raw materials of which include fermentation concentrate of starch raw materials, inorganic fertilizer and nematophilic Serratia KWNj-P.

[0008] Furthermore, the fermentation concentrate of the starchy raw material is derived from the production of citric acid.

[0009] Furthermore, the starchy raw material includes at least one of corn, cassava and potato.

[0010] Furthermore, the inorganic fertilizer includes one or more of urea, diammonium phosphate, and potassium sulfate.

[0011] Furthermore, the inorganic fertilizer is added in an amount of 90-174 g / L relative to the starchy raw material fermentation concentrate.

[0012] Furthermore, the total nutrient content of the biological organic water-soluble fertilizer is 8% to 12%, the organic matter content is 20-22%, and the effective viable bacteria count is 5×10 7 -5×10 8 CFU / mL, pH 5.5-6.5.

[0013] A process for preparing the novel biological organic water-soluble fertilizer comprises the following steps:

[0014] (1) Preparing starchy raw material fermentation concentrate: using starchy raw material fermentation filtrate as raw material, filtering and concentrating the starchy raw material fermentation concentrate through plate and frame filtration;

[0015] (2) Adding auxiliary materials and bacterial solution: adding inorganic fertilizer and bacterial solution of Serratia nematophila KWNj-P to the fermentation concentrate obtained in step (1), and stirring evenly to obtain a novel biological organic water-soluble fertilizer.

[0016] An application of the novel biological organic water-soluble fertilizer in preventing and controlling bacterial wilt of crops.

[0017] The beneficial technical effects of the present invention are:

[0018] (1) The raw materials of the novel biological organic water-soluble fertilizer of the present invention are complementary to each other, containing a large amount of nutrient elements and organic matter that is beneficial to plant growth. After compounding with microbial agents, a comprehensive organic-inorganic full-element biological fertilizer is formed to maintain nutrient balance; the component nematophilic Serratia KWNj-P in the novel biological organic water-soluble fertilizer of the present invention belongs to rhizosphere growth-promoting bacteria, which can improve soil and enhance plant resistance to stress. At the same time, after the novel biological organic water-soluble fertilizer is diluted and irrigated to the roots of crops, the plants show significant resistance to diseases and pests, increase crop yields, and improve crop quality. Moreover, it is green, safe, and has no pollution to the environment;

[0019] (2) The fermentation concentrate in the novel biological organic water-soluble fertilizer of the present invention is industrial waste, which has low cost and can be reused to turn waste into treasure, which is environmentally friendly;

[0020] (3) The novel biological organic water-soluble fertilizer of the present invention can be dissolved in water and then applied in an integrated manner, or can be applied directly, thus adapting to the diverse modern agricultural production methods. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 This chart shows the growth-promoting effects of different fertilizers on tomatoes.

[0022] Figure 2 Effects of different treatments on the disease index of tomato bacterial wilt.

[0023] In the figure: A, disease index on day 8-20; B, disease index on day 20. DETAILED DESCRIPTION

[0024] The present invention will be described in detail below in conjunction with the embodiments.

[0025] The nematophilic Serratia used in the following embodiments is the nematophilic Serratia disclosed in Chinese invention patent CN202310679979.9, and its classification name is Serratia sp.KWNj-P, which was deposited in the China Center for Type Culture Collection on April 4, 2023 with the deposit number of CCTCC NO: M 2023425.

[0026] The test tomatoes in the following examples were purchased from Jiangsu Academy of Agricultural Sciences, and the variety was Hezuo 903.

[0027] The starchy raw material fermentation filtrates in the following embodiments include corn fermentation filtrate, cassava fermentation filtrate, and potato fermentation filtrate, all of which are produced in the citric acid production process and are obtained from Jiangsu Guoxin Xielian Biotechnology (Group) Co., Ltd.

[0028] The experimental methods in the following examples are all conventional methods unless otherwise specified.

[0029] Unless otherwise specified, the materials and reagents used in the following examples can be obtained from commercial sources.

[0030] The preparation method of the Serratia nematophila KWNj-P bacterial solution in the following examples is as follows:

[0031] Preparation of seed solution: inoculate strain KWNj-P onto LB solid medium, culture at 30°C for 24 h, pick a single colony into a 50 mL conical flask containing 20 mL LB liquid medium, and culture at 30°C, 170 r / min on a shaker for 12 h to obtain seed solution;

[0032] Fermentation culture: inoculate the seed liquid into LB liquid culture medium at an inoculation rate of 1% (volume percentage), culture at 30°C and 170 r / min on a shaker for 24-36 hours to obtain the fermentation liquid of strain KWNj-P, then centrifuge at 3000 r / min for 10 minutes, remove the supernatant, collect the bacteria, and resuspend the bacteria with sterile water to obtain the nematophilic Serratia marcescens KWNj-P bacterial liquid.

[0033] The configuration methods of the LB solid medium, LB liquid medium, NB medium, and NA medium used are as follows:

[0034] LB solid medium: 10 g of tryptone, 10 g of sodium chloride, 5 g of yeast extract, 20 g of agar, and 1000 mL of deionized water. Adjust the pH to 6.5-7.0 with NaOH, and sterilize at 115°C with high-pressure steam for 30 min.

[0035] LB liquid culture medium: 10 g of tryptone, 10 g of sodium chloride, 5 g of yeast extract, 1000 mL of deionized water, adjust the pH to 6.5-7.0 with NaOH, sterilize at 115°C with high-pressure steam for 30 min.

[0036] NB liquid culture medium: 5 g tryptone, 10 g glucose, 0.5 g yeast extract, 3 g beef extract, 1000 mL deionized water, adjust the pH to 6.5-7.0 with NaOH, sterilize at 115°C with high pressure steam for 30 min.

[0037] NA solid medium: Add 20 g / L agar to NB medium.

[0038] Example 1

[0039] A novel biological organic water-soluble fertilizer, the preparation method of which is as follows:

[0040] (1) Preparation of starchy raw material fermentation concentrate: The starchy raw material fermentation filtrate was used as the raw material, and the residue was removed after plate and frame filtration, and then the starchy raw material fermentation concentrate was obtained by MVR concentration. The basic properties of the starchy raw material fermentation concentrate are shown in Table 1.

[0041] Table 1 Basic properties of starchy raw material fermentation concentrate

[0042]

[0043] (2) Adding auxiliary materials and bacterial solution: 52.0 g urea, 27.9 g diammonium phosphate, and 8.3 g potassium sulfate were added to 1 L of the fermentation concentrate of the starchy raw material obtained in step (1) to adjust the total nutrient content (N+P2O5+K2O) to 8%, wherein N was 3.7%, P2O5 was 2.4%, and K2O was 1.9%. The pH was adjusted to 5.5 using KOH at a concentration of 10 mol / L; and the bacterial solution of Serratia nematophila KWNj-P was added to adjust the effective viable count to 5×10 7 CFU / mL, and after stirring evenly, a new type of biological organic water-soluble fertilizer was obtained.

[0044] Example 2

[0045] A novel biological organic water-soluble fertilizer, the preparation method of which is as follows:

[0046] (1) Preparation of starchy raw material fermentation concentrate: The starchy raw material fermentation filtrate was used as the raw material, and the residue was removed after plate and frame filtration, and then the starchy raw material fermentation concentrate was obtained by MVR concentration. The basic properties of the starchy raw material fermentation concentrate are shown in Table 1.

[0047] (2) Adding auxiliary materials and bacterial solution: 79.1 g urea, 34.5 g diammonium phosphate, and 13.0 g potassium sulfate were added to 1 L of the fermentation concentrate of the starchy raw material obtained in step (1) to adjust the total nutrient content (N+P2O5+K2O) to 10%, wherein N was 5.04%, P2O5 was 2.8%, and K2O was 2.15%. The pH was adjusted to 6.0 using KOH at a concentration of 10 mol / L; and the bacterial solution of Serratia nematophila KWNj-P was added to adjust the effective viable count to 5×10 7 CFU / mL, and after stirring evenly, a new type of biological organic water-soluble fertilizer was obtained.

[0048] Example 3

[0049] A novel biological organic water-soluble fertilizer, the preparation method of which is as follows:

[0050] (1) Preparation of starchy raw material fermentation concentrate: The starchy raw material fermentation filtrate was used as the raw material, and the residue was removed after plate and frame filtration, and then the starchy raw material fermentation concentrate was obtained by MVR concentration. The basic properties of the starchy raw material fermentation concentrate are shown in Table 1.

[0051] (2) Adding auxiliary materials and bacterial solution: 106.5 g urea, 48.6 g diammonium phosphate, and 18.5 g potassium sulfate were added to 1 L of the fermentation concentrate of the starchy raw material obtained in step (1) to adjust the total nutrient content (N+P2O5+K2O) to 12%, wherein N was 5.94%, P2O5 was 3.68%, and K2O was 2.45%. The pH was adjusted to 6.5 using KOH at a concentration of 10 mol / L; and the bacterial solution of Serratia nematophila KWNj-P was added to adjust the effective viable count to 5×10 7 CFU / mL, and after stirring evenly, a new type of biological organic water-soluble fertilizer was obtained.

[0052] Comparative Example 1

[0053] A fertilizer, the preparation method of which is as follows:

[0054] 69.3 g of urea, 38.9 g of ammonium dihydrogen phosphate, and 29.6 g of potassium sulfate were dissolved in 1 L of water to obtain a fertilizer having a total nutrient content (N+P2O5+K2O) of 8%, of which N was 3.7%, P2O5 was 2.4%, and K2O was 1.9%.

[0055] Comparative Example 2

[0056] An organic water-soluble fertilizer, the preparation method of which is as follows:

[0057] (1) Preparation of starchy raw material fermentation concentrate: same as step (1) in Example 1.

[0058] (2) Adding auxiliary materials: 52.0 g urea, 27.9 g diammonium phosphate, and 8.3 g potassium sulfate were added to 1 L of the starchy raw material fermentation concentrate obtained in step (1) to adjust the total nutrient content (N+P2O5+K2O) to 8%, wherein N is 3.7%, P2O5 is 2.4%, and K2O is 1.9%.

[0059] Test Case

[0060] (1) Growth-promoting effect of new biological organic water-soluble fertilizer and influence of soil

[0061] The growth-promoting effect of the new biological organic water-soluble fertilizer on tomato plants was verified through greenhouse potted experiments.

[0062] The pot experiment was conducted in the greenhouse of Jiangsu Guoxin Xielian Energy Co., Ltd. Four treatments (9 replicates for each treatment) were set as follows:

[0063] Treatment 1 (T1): application of clean water;

[0064] Treatment 2 (T2): application of the fertilizer prepared in Comparative Example 1;

[0065] Treatment 3 (T3): applying the organic water-soluble fertilizer prepared in Comparative Example 2;

[0066] Treatment 4 (T4): application of the novel bio-organic water-soluble fertilizer prepared in Example 1;

[0067] Vermiculite and soil (volume ratio 2:1) were placed in plastic flower pots, 800g per pot; tomatoes were first grown with seedling medium (purchased from Xiangzheng Agricultural Science), and transplanted into flower pots after one true leaf grew out. Fertilization began 2-3 days after transplantation, and the fertilization method was root irrigation. 2mL of fertilizer was applied to each pot each time. The fertilizer was diluted 100 times and applied to the roots of the crops. It was applied once every 6 days, for a total of 5 times, in the evening or morning. After the fertilization was completed, the growth of the plants was observed. The results were as follows: Figure 1 And as shown in Table 2.

[0068] Table 2 Effects of different fertilizer treatments on biological traits of tomatoes

[0069]

[0070] Note: Different lowercase letters in the same column indicate significant differences at the P<0.05 level

[0071] from Figure 1 As can be seen from the results in Table 2, in terms of tomato stem thickness, plant height, aboveground fresh weight and dry weight, underground fresh weight and dry weight, the new biological organic water-soluble fertilizer (T4) treatment is significantly better than the chemical fertilizer (T2) and water (T1) treatment; in terms of stem thickness, aboveground dry weight, underground fresh weight, underground dry weight, the new biological organic water-soluble fertilizer (T4) treatment is significantly higher than the other three treatments. The results show that the new biological organic water-soluble fertilizer prepared by the present invention can significantly improve the biological traits of crops.

[0072] At the same time, the contents of soil alkaline nitrogen, available phosphorus and available potassium were measured and statistically analyzed. The results are shown in Table 3.

[0073] Table 3 Effects of different fertilizer treatments on soil

[0074]

[0075] Note: Different lowercase letters in the same column indicate significant differences at the P<0.05 level

[0076] From the results in Table 3, it can be seen that the T4 treatment is significantly better than the other three treatments in terms of soil alkaline nitrogen, available phosphorus and available potassium. The results show that the novel biological organic water-soluble fertilizer prepared by the present invention can significantly improve the physical and chemical properties of soil.

[0077] (2) Disease resistance effect of new biological organic water-soluble fertilizer

[0078] The bacterial suspension of Raslotniao solanacearum (R. solanacearum) was prepared as follows:

[0079] Preparation of seed solution: streak the strain R. solanacearum from the -80℃ glycerol tube onto a plate of NA solid culture medium and culture it in a 30℃ incubator for 24 hours; pick a single colony of R. solanacearum on the plate into a 50mL (NB liquid culture medium is filled with 20mL) conical flask and culture it at 30℃, 170r / min with shaking for 12 hours to obtain the seed solution.

[0080] Preparation of bacterial suspension: inoculate the seed liquid into NB liquid culture medium at an inoculum rate of 1% (v / v), place it at 30°C and shake on a shaker at 170 r / min for 24-36 h to obtain the fermentation broth of strain R. solanacearum, centrifuge it at 3000 r / min for 10 min to remove the supernatant, collect the cells, and resuspend the cells in an equal volume of sterile water to obtain the bacterial suspension of strain R. solanacearum.

[0081] The effect of the new biological organic water-soluble fertilizer on the resistance of tomato plants to bacterial wilt was verified through greenhouse pot experiments.

[0082] The pot experiment was conducted in the greenhouse of Jiangsu Guoxin Xielian Energy Co., Ltd. Four treatments (9 replicates for each treatment) were set as follows:

[0083] Treatment 1 (T1): application of clean water;

[0084] Treatment 2 (T2): application of the fertilizer prepared in Comparative Example 1;

[0085] Treatment 3 (T3): applying the organic water-soluble fertilizer prepared in Comparative Example 2;

[0086] Treatment 4 (T4): application of the novel bio-organic water-soluble fertilizer prepared in Example 1;

[0087] Vermiculite and soil (volume ratio 2:1) were placed in plastic flower pots, with 800 g per pot. Tomato seedlings were first grown with seedling medium (purchased from Xiangzheng Agricultural Science), and transplanted into flower pots after growing one true leaf. Fertilization began 2-3 days after transplanting. The fertilization method was root irrigation. 2 mL of fertilizer was applied to each pot each time. The fertilizer was diluted 100 times and applied to the roots of the crop. It was applied once every 6 days for a total of 5 times in the evening or morning. At the same time, two weeks after the tomato was transplanted, the pathogen (Ralstonia solanacearum) was inoculated by root irrigation. The amount of Ralstonia solanacearum RS added was 2×10 6 After inoculation of pathogens, maintain high temperature and high humidity, and observe and count the incidence and disease status of tomatoes every two days.

[0088] The disease severity of the plants is divided into numbers from 0 to 4 (0: no disease symptoms; 1: less than 1 / 4 of the plant has disease symptoms; 2: 1 / 4 to 1 / 2 of the plant has disease symptoms; 3: 1 / 2 to 3 / 4 of the plant has disease symptoms; 4: more than 3 / 4 of the plant has disease symptoms.)

[0089] Disease index (%) = [∑ (number of diseased plants × representative value) / (total number of plants × representative value of the most severe disease level)] × 100.

[0090] The results are as follows Figure 2 As shown. Figure 2 As can be seen from Figure A, the pathogen was inoculated by root irrigation two weeks after eggplant transplanting. The tomato plants began to become sick on the 8th day after inoculation, and the disease conditions of the tomato plants were observed and recorded at noon every two days. The tomato bacterial wilt disease index of the treatment with the new biological organic water-soluble fertilizer (T4) within 12 days of the onset of the disease was basically lower than that of the T3, T2, and T1 treatments. On the 20th day, the treatment T1 (clear water control) with the highest disease index reached more than 57%. The disease of the T4 treatment was stable, and the disease statistics were ended. Figure 2 As can be seen from Figure B, the bacterial wilt of tomatoes in the T1 treatment was the most serious. Compared with the T1 treatment, the bacterial wilt disease index of the T2 treatment, T3 treatment and T4 treatment decreased by 10.50%, 18.23% and 37.27% respectively, and the bacterial wilt disease index of tomatoes in the T4 treatment was significantly lower than that of the T1 treatment, T2 treatment and T3 treatment. The results show that the application of the novel biological organic water-soluble fertilizer containing the nematophilic Serratia KWNj-P prepared by the present invention has the best control effect on tomato bacterial wilt.

[0091] The above is only a preferred embodiment of the present invention, and the present invention is not limited to the above embodiments. It is understood that other improvements and changes directly derived or associated by those skilled in the art without departing from the spirit and concept of the present invention should be considered to be included in the protection scope of the present invention.

Claims

1. A novel biological organic water-soluble fertilizer, characterized in that: The raw materials of the novel biological organic water-soluble fertilizer include fermentation concentrate of starch raw materials, inorganic fertilizer and nematophilic Serratia KWNj-P.

2. The novel biological organic water-soluble fertilizer according to claim 1, characterized in that: The starchy raw material fermentation concentrate is derived from the production of citric acid.

3. The novel biological organic water-soluble fertilizer according to claim 2, characterized in that: The starchy raw material includes at least one of corn, cassava and potato.

4. The novel biological organic water-soluble fertilizer according to claim 1, characterized in that: The inorganic fertilizer includes one or more of urea, diammonium phosphate, and potassium sulfate.

5. The novel biological organic water-soluble fertilizer according to claim 1, characterized in that: The inorganic fertilizer is added in an amount of 90-174 g / L relative to the starch raw material fermentation concentrate.

6. The novel biological organic water-soluble fertilizer according to claim 1, characterized in that: The total nutrient content of the biological organic water-soluble fertilizer is 8% to 12%, the organic matter content is 20-22%, and the effective living bacteria count is 5×10 7 -5×10 8 CFU / mL, pH 5.5-6.

5.

7. A process for preparing the novel biological organic water-soluble fertilizer according to claim 1, characterized in that: The process comprises the following steps: (1) Preparing starchy raw material fermentation concentrate: using starchy raw material fermentation filtrate as raw material, filtering and concentrating the starchy raw material fermentation concentrate through plate and frame filtration; (2) Adding auxiliary materials and bacterial solution: adding inorganic fertilizer and bacterial solution of Serratia nematophila KWNj-P to the fermentation concentrate obtained in step (1), and stirring evenly to obtain a novel biological organic water-soluble fertilizer.

8. Use of the novel bio-organic water-soluble fertilizer according to any one of claims 1 to 6 in preventing and controlling bacterial wilt of crops.

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