Microbial preparation containing arbuscular mycorrhizal fungi and application thereof

Abstract

The invention relates to a microbial preparation containing arbuscular mycorrhizal fungi and application thereof, and belongs to the technical field of plant cultivation. According to the application disclosed by the invention, it is found that all of the glomus amarae, the rhizopus intro-rhizosporium, the glomus mosseae and the cyst macrosporium in the arbuscular mycorrhizal fungi can promote the growth of plants under the condition of double pollution of molybdenum and micro-plastics, and the plant height and the grain yield of the plants are effectively improved. Further experiments find that under the same condition, the effect is better than that of applying a single arbuscular mycorrhizal fungus inoculant when the near-phoma globosum, the rhizosporium intraradices and the glomus mosseae or the mixed inoculant of the near-phoma globosum, the glomus mosseae and the cyst macrosporium is applied. Furthermore, after beneficial bacteria and auxiliary materials are added into the mixed bacterial agent, the mixed bacterial agent has a better promoting effect on plant growth under the condition of double pollution of molybdenum and micro-plastics. The microbial preparation provided by the invention can reduce poison of microplastics and molybdenum to plants, so that the plants can normally grow under dual pollution.

Description

Technical Field

[0001] The invention relates to a microbial preparation containing arbuscular mycorrhizal fungi and application thereof, belonging to the technical field of plant cultivation. Background Art

[0002] Molybdenum is a transition element and a trace element necessary for the growth of biological organisms including plants. However, excessive intake of molybdenum can lead to poisoning. Although plants are highly resistant to molybdenum, when a high level of molybdenum appears in the plant body, it will also have a toxic effect on the plant. In addition, high concentrations of molybdenum will reduce the quality of crops, while excessive molybdenum will inhibit the photosynthesis and transpiration of plants, destroy the active oxygen metabolism mechanism at a deeper level, and cause membrane lipid peroxidation, ultimately affecting the normal growth of plants. Studies have shown that excessive molybdenum can cause the leaves of tomatoes and cabbages to turn purple, and the leaves of soybeans to turn yellow; inhibit the growth of the roots and aboveground parts of chickpeas, and change the cross-sectional structure of their roots, leaves and stems; reduce the chlorophyll content of winter wheat, resulting in a decrease in biological yield. Moreover, more and more studies have shown that higher concentrations of molybdenum pollution (3000-4000 mg / kg) can lead to direct crop death.

[0003] Microplastics (MPs) refer to plastic particles or fibers with a diameter of less than 5 mm, which are widely used in various fields, such as agricultural microplastic covering films, decomposition products of plastic waste, etc. Microplastics can be adsorbed on the surface of heavy metals, both of which will destroy the ecological balance of the soil, inhibit the activity and diversity of soil microorganisms, affect the nutrient cycle and organic matter decomposition of the soil, and also have a negative impact on plant growth (such as reducing crop yields and crop quality), seriously threatening the safety of the ecological environment. Therefore, on the one hand, improving the pollution of molybdenum (heavy metals) and microplastics to the environment, and on the other hand, improving the resistance of crops to molybdenum and microplastics, are of great significance to promoting the growth of crops in contaminated soil, alleviating the toxicity caused by microplastics and molybdenum, and improving crop height, yield and other indicators.

[0004] Mycorrhiza is a highly evolved mutualistic symbiosis formed by the symbiosis of fungi and terrestrial vascular plant roots in the soil. Fungi that can form this symbiotic relationship with plant roots are called mycorrhizal fungi. It has both the characteristics of general plant roots and the characteristics of specialized fungi, and is widely distributed in nature. At present, seven types of mycorrhiza have been found, including arbuscular mycorrhizae, ectomycorrhizae, ectomycorrhizae, orchid mycorrhizae, heather or azalea mycorrhizae, crystal orchid mycorrhizae, and berry mycorrhizae. Among these mycorrhizae types, arbuscular mycorrhizae (AM) is the most widely distributed type of mycorrhizae in terrestrial ecosystems and the one that is most closely related to plants. After arbuscular mycorrhizal fungi infect the root system, they will enter the cortical cells of the root system and form a dichotomous branching structure, thus becoming arbuscular mycorrhizae. This type of fungus is arbuscular mycorrhizal fungi.

[0005] Arbuscular mycorrhizae (AM) can not only improve plant nutrition, but also greatly promote plant resistance to stress. In addition, AM fungi can improve soil physical and chemical properties, promote plant water and nutrient absorption and utilization, and promote plant resistance to heavy metals, thereby reducing the damage caused by heavy metal stress to plants. Studies have shown that AM fungi can alleviate the adverse effects of microplastics on soil nutrient availability and plant nutrient absorption. AM fungi hyphae can capture microplastics and further transport them to the roots. AM fungi secretions can promote the aggregation of microplastics, thereby reducing their absorption.

[0006] A Chinese invention patent with an authorization announcement date of August 20, 2019 and an authorization announcement number of CN108419605B discloses an arbuscular mycorrhizal fungal agent and its preparation method and application. It specifically discloses a method for preparing an arbuscular mycorrhizal fungal agent, and the prepared arbuscular mycorrhizal fungal agent can promote the growth of corn in molybdenum-contaminated soil.

[0007] In the joint detoxification effect of arbuscular mycorrhizal fungi and earthworms on soil microplastic pollution published by He Baiping in 2022, it is recorded that in soil ecosystems, arbuscular mycorrhizal (AM) fungi and earthworms play an important role in improving plant nutrition levels and enhancing plant stress resistance. It specifically discloses that microplastics have an adverse effect on the growth of soil AM fungi and peppers, and explains that AM fungi and earthworms have a positive effect on reducing the plant biotoxicity of soil microplastic pollution. The synergistic effect of earthworms and AM fungi has great potential in promoting pepper growth.

[0009] Although the above-mentioned articles or Chinese invention patents disclose that arbuscular mycorrhizal fungi can improve the yield and quality of plants by improving the nutritional status of plants, and can also improve the resistance of crops to adverse stresses such as heavy metals and microplastics, they do not involve the use of arbuscular mycorrhizal fungi under the dual stress of microplastics and molybdenum. There are also no reports or patent disclosures on the use of microbial preparations containing arbuscular mycorrhizal fungi to promote the growth of crops under the dual pollution conditions of microplastics and molybdenum. Summary of the invention

[0010] The first purpose of the present invention is to provide a microbial preparation containing arbuscular mycorrhizal fungi, which provides a microbial preparation for the prior art that can effectively promote plant growth under conditions of dual pollution of microplastics and molybdenum.

[0011] The second object of the present invention is to provide an application of a microbial preparation in promoting plant growth and development in soil doubly contaminated by microplastics and molybdenum, so as to solve the current problem of poor plant growth in an environment contaminated by molybdenum and microplastics.

[0012] In order to achieve the above object, the technical scheme of a microbial preparation containing arbuscular mycorrhizal fungi in the present invention is:

[0013] A microbial preparation containing arbuscular mycorrhizal fungi, wherein the microbial preparation includes an arbuscular mycorrhizal fungal agent; the arbuscular mycorrhizal fungal agent is a young globus sac agent, an intraradical sporangiophore and a Moses globus sac agent or a young globus sac agent, a Moses globus sac agent and a branched giant sporangiophore; the propagule number ratio of the young globus sac agent, the intraradical sporangiophore and the Moses globus sac agent is (1-1.1):(0.7-0.9):(1.3-1.4); the propagule number ratio of the young globus sac agent, the Moses globus sac agent and the branched giant sporangiophore is (0.9-1.1):(1.2-1.3):(0.8-1.0).

[0014] The beneficial effect of the above technical solution is that the microbial preparation containing arbuscular mycorrhizal fungi of the present invention is a combined invention. The present invention has found through a large number of field experiments in the early stage that under the conditions of dual pollution of molybdenum and microplastics, the growth of plants is significantly inhibited, affecting the plant height, grain yield, etc. of the plants. It is found through experiments that the young arbuscular mycorrhizal fungi Claroideoglomus etunicatum, Rhizophagusintraradices, Glomus mosseae, and Gigasporaramisporophora can promote the growth of plants under the conditions of dual pollution of molybdenum and microplastics, and effectively improve the plant height and grain yield of the plants. Moreover, it is found through further experiments that under the same conditions, the mixed inoculum of Claroideoglomus etunicatum, Rhizophagusintraradices and Glomus mosseae or Claroideoglomus etunicatum, Glomus mosseae and Gigasporaramisporophora is better than the application of a single arbuscular mycorrhizal fungi agent. This shows that the above-mentioned combination of bacterial agents has a synergistic effect, which can effectively promote the growth of plants under conditions of dual pollution of molybdenum and microplastics, and improve plant height, yield and other indicators. It provides an effective microbial preparation for increasing plant yield under conditions of dual pollution of molybdenum and microplastics, and also lays the foundation for subsequent research to simultaneously reduce the toxicity of microplastics and molybdenum to plants.

[0015] Specifically, the preparation process of the arbuscular mycorrhizal fungal agent is: preparing a mixed matrix for culture, inoculating the arbuscular mycorrhizal fungal inoculant into the mixed matrix, and spreading it in the field to coexist with plants for 2 to 3 months, harvesting the plant roots and mixing them with the mixed matrix to obtain a mixture consisting of spores, hyphae, root fragments and the mixed matrix, which is the arbuscular mycorrhizal fungal agent.

[0016] As a further improvement, the number of propagules in the arbuscular mycorrhizal fungal agent is not less than 110 per gram.

[0017] As a further improvement, the propagules are composed of spores, extraradical hyphae and infected root segments.

[0018] Specifically, in theory, any one of the spores, extraradical hyphae and infected root segments of arbuscular mycorrhizal fungi can act as an arbuscular mycorrhizal fungal agent. However, in practical applications, for the convenience of preparation, the spores, extraradical hyphae and infected root segments of arbuscular mycorrhizal fungi are often mixed together with a mixed matrix as an arbuscular mycorrhizal fungal agent.

[0019] As a further improvement, the young set of nearly bright spore mold agent, root root spore mold agent, Moses spore mold agent and branched giant spore mold agent meet the following standards: the number of spores is not less than 6 / gram, the extraroot mycelium density is not less than 18m / g, and the infected root segment is not less than 20cm / g.

[0020] As a further improvement, the microbial preparation includes Bacillus subtilis, Trichoderma longifolia, polyglutamic acid, humic acid and ammonium molybdate.

[0021] The beneficial effect of the above technical solution is that: the present invention has found through further experiments that after the arbuscular mycorrhizal fungal agent is mixed with the above components and applied to plants, the effect is better than that of a single arbuscular mycorrhizal fungal agent.

[0022] As a further improvement, the mass ratio of the arbuscular mycorrhizal fungi agent, Bacillus subtilis, Trichoderma longifolia, polyglutamic acid, humic acid and ammonium molybdate is 85:(4-5):(4-5):(2-3):(2-3):(0.04-0.05).

[0023] As a further improvement, the effective viable count of the Bacillus subtilis is not less than 4×10 8 CFU / g, the effective viable count of Trichoderma longifolia is not less than 8×10 8 CFU / g.

[0024] In order to achieve the above-mentioned purpose, the technical scheme of the present invention for using a microbial preparation in promoting plant growth and development in soils that are double-contaminated by microplastics and molybdenum is:

[0025] Application of a microbial preparation in promoting plant growth and development in soil dual-contaminated by microplastics and molybdenum.

[0026] The beneficial effect of the above technical solution is that: the present invention uses microbial preparations containing arbuscular mycorrhizal fungi to apply to the cultivation of crops in microplastic and molybdenum contaminated soil, which can significantly increase the plant height, yield, etc., promote plant growth, reduce the toxicity of microplastics and molybdenum to plants, and enable crops to grow normally in soil with dual stress of microplastics and molybdenum.

[0027] As a further improvement, the growth and development is plant height, yield and / or chlorophyll content.

[0028] As a further improvement, the plants include wheat, mung beans, and corn.

[0029] Compared with the prior art, the present invention has the following beneficial effects:

[0030] (1) The present invention establishes a symbiotic relationship between arbuscular mycorrhizal fungi and host plants, uses the hyphae of arbuscular mycorrhizal fungi to help plants obtain nutrients, and improves the growth of crops in soil contaminated by microplastics and molybdenum;

[0031] (2) The mixed arbuscular mycorrhizal fungal agent used in the present invention has high activity, can effectively adjust soil structure, improve soil water and fertilizer retention capacity, promote the growth of crops in polluted soil, alleviate the toxicity caused by microplastics and molybdenum, improve plant height, yield and other indicators, promote plant growth, and achieve the beneficial effect of promoting crop growth;

[0032] (3) The present invention adds Bacillus subtilis, Trichoderma longifolia, polyglutamic acid, humic acid and ammonium molybdate to the microbial preparation. Bacillus subtilis and Trichoderma longifolia promote seed germination, improve seed disease resistance, improve fertilizer utilization rate, and enhance seed resistance to soil-borne diseases and soil harmful bacteria; adding polyglutamic acid can effectively maintain soil moisture, improve soil structure and aeration, and improve soil water and fertilizer retention capacity; humic acid has the effects of improving soil structure, improving fertilizer efficiency, and improving crop resistance to stress; adding ammonium molybdate can not only promote crop absorption of phosphorus but also improve the ability of arbuscular mycorrhizal fungi to cope with molybdenum stress from the source;

[0033] (4) The preparation process of the microbial preparation of the present invention is simple and easy, easy to operate, environmentally friendly and harmless, and has no side effects. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] Figure 1 This is a graph of plant height, grain P content, and grain yield of mung beans applied with a microbial preparation containing arbuscular mycorrhizal fungi and without applying a microbial preparation containing arbuscular mycorrhizal fungi under microplastic and molybdenum pollution conditions in Example 4 of the present invention (different letters indicate significant differences, and the same letters indicate no significant differences; AM1 is a microbial preparation containing a mixed arbuscular mycorrhizal fungi of Glomus juvenile, Glomus endoradici and Glomus mosseae; AM2 is a microbial preparation containing a mixed arbuscular mycorrhizal fungi of Glomus juvenile, Glomus mosseae and Glomus mosseae);

[0035] Figure 2 Figure 5 is a graph of corn plant height, grain P content, and grain yield when a microbial preparation containing arbuscular mycorrhizal fungi is applied and when a microbial preparation containing arbuscular mycorrhizal fungi is not applied under conditions of microplastic and molybdenum pollution in Example 5 of the present invention (different letters indicate significant differences, and the same letters indicate no significant differences; AM1 is a microbial preparation containing mixed arbuscular mycorrhizal fungi of Glomus juvenile, Glomus endoradici and Glomus mosseae; AM2 is a microbial preparation containing mixed arbuscular mycorrhizal fungi of Glomus juvenile, Glomus mosseae and Giant Sporangium branching);

[0036] Figure 3Figure 6 is a graph of wheat plant height, grain P content, and grain yield when a microbial preparation containing arbuscular mycorrhizal fungi is applied and when a microbial preparation containing arbuscular mycorrhizal fungi is not applied under conditions of microplastic and molybdenum pollution in Example 6 of the present invention (different letters indicate significant differences, and the same letters indicate no significant differences; AM1 is a microbial preparation containing mixed arbuscular mycorrhizal fungi of Glomus juvenile, Glomus endoradici and Glomus mosseae; AM2 is a microbial preparation containing mixed arbuscular mycorrhizal fungi of Glomus juvenile, Glomus mosseae and Giant Sporangium branching);

[0037] Figure 4 This is a graph of corn yield when a microbial preparation containing arbuscular mycorrhizal fungi was applied and when a microbial preparation containing arbuscular mycorrhizal fungi was not applied under conditions of microplastic and molybdenum pollution in the field experiment in Example 7 of the present invention (different letters indicate significant differences, and the same letters indicate no significant differences; AM1 is a microbial preparation containing a mixed arbuscular mycorrhizal fungi of Glomus juvenile, Glomus endoradici and Glomus mosseae; AM2 is a microbial preparation containing a mixed arbuscular mycorrhizal fungi of Glomus juvenile, Glomus mosseae and Giant Sporangiophore). DETAILED DESCRIPTION

[0038] Microplastics and molybdenum-contaminated soil can inhibit the development of plant roots and their absorption of nutrients, thus affecting the growth of the entire plant. Microplastics can be adsorbed on the surface of heavy metals (molybdenum), which in turn exacerbates the impact on soil quality, thereby reducing crop yields, reducing their quality, and threatening ecological and environmental safety. Arbuscular mycorrhizal fungi can form a mutualistic symbiotic system with most terrestrial plants, playing an important role in improving plant nutrition, maintaining soil health and ecosystem stability. The culture matrix containing arbuscular mycorrhizal fungi has the characteristics of good air permeability and water retention, which can meet the needs of crops for water, fertilizer and oxygen.

[0039] The present invention first provides a microbial preparation containing arbuscular mycorrhizal fungi, which utilizes the synergistic effect between Glomus juvenile, Rhizocystis endoradiata and Glomus mosseae or Glomus juvenile, Glomus mosseae and Giant Sporangium ramification to better improve the growth of crops in soil contaminated by microplastics and molybdenum, and increase the plant height and grain yield of plants. The microbial preparation of the present invention provides a fertilizer that can increase crop yield in soils contaminated by microplastics and molybdenum, laying a foundation for subsequent fertilizer research and development.

[0040] Furthermore, the present invention provides the use of a microbial preparation containing arbuscular mycorrhizal fungi in promoting plant growth and development in soil doubly contaminated by microplastics and molybdenum. The present invention has previously proved that applying the microbial preparation of the present invention to soil doubly contaminated by molybdenum and microplastics can effectively improve the nutritional status and yield of crops, while being environmentally friendly and able to achieve sustainable development.

[0041] The present invention is further described in detail below in conjunction with specific examples. Unless otherwise specified, the equipment and reagents used in each embodiment, experimental example and comparative example can be obtained from commercial sources.

[0042] In the following embodiments of the present invention, the characteristics of the strains of Claroideoglomus etunicatum, Rhizophagus intraradices, Glomus mosseae and Gigaspora ramisporophora are used. The strains obtained by conventional means meet the requirements of the embodiments (such as the number of propagules) and can obtain the same or similar effects as those in the embodiments. Ammonium molybdate, microplastics, Bacillus subtilis, Trichoderma longifolia, polyglutamic acid, humic acid and crop seeds are all purchased from the market.

[0043] 1. A specific embodiment of a microbial preparation containing arbuscular mycorrhizal fungi according to the present invention:

[0044] Example 1 Preparation of arbuscular mycorrhizal fungal agent

[0045] The preparation process of the arbuscular mycorrhizal fungi agent of the present embodiment is as follows:

[0046] Clay granules and bagasse were crushed and passed through a 2mm sieve. Then, ceramsite and bagasse were mixed in a mass ratio of 3:1 to obtain a mixed matrix. The mixed matrix was sterilized at 121℃ for 2h, cooled and dried naturally for use. A mixed soil sample containing spores, hyphae and infected root segments of the young glomerulone fungus was used as an inoculant for the young glomerulone fungus. The young glomerulone fungus inoculant was inoculated into the mixed matrix and mixed evenly. The matrix was spread to a thickness of 10-20cm, watered to 50-70% of the field water holding capacity, and corn seeds were sown. After sowing, the surface was covered with 1-2cm of mixed matrix. Cultured in an artificial climate chamber for 2-3 months, and Hoagland nutrient solution was irrigated once every 2 weeks during the growth and culture process. The culture conditions of the artificial climate chamber are to ensure daily light of 8000-12000lux, temperature of 28-35℃ for 13-14h, and darkness of 18-25℃ for 10-11h. Then the above-ground part of the corn is harvested and cut off, and the root system is cut into pieces less than 0.5 cm and then fully mixed with the mixed matrix to obtain a mixture (soil) consisting of spores, hyphae, root fragments and mixed matrix, which is the young set of Near-Ming spherical mold agent.

[0047] Note: The mass of spores, hyphae, and root fragments is negligible relative to the mixed matrix.

[0048] The method for preparing the mossea sac mold agent, the root cyst mold agent and the branched giant cyst mold agent is similar to the method for preparing the above-mentioned young set of sac mold agent, except that the young set of sac mold is replaced by mossea sac mold, the root cyst mold or the branched giant cyst mold.

[0049] Example 2 Screening of arbuscular mycorrhizal fungal agents

[0050] The screening process of the arbuscular mycorrhizal fungi agent of the present embodiment is as follows:

[0051] The young glomerulonephritis fungi (fungus a), the root spore fungi (fungus b), the Moses glomerulonephritis fungi (fungus c) and the branched giant spore fungi (fungus d) prepared in Example 1 were added to the soil containing ammonium molybdate (100 mg / kg) and polyvinyl chloride (PVC) (1.2% w / w) and mixed evenly. The added fungi must meet the following two standards at the same time: (1) the total number of propagules per dose is not less than 60 / g soil; (2) the number of spores per dose is not less than 6 / g soil, the mycelium density is not less than 18m / g soil, and the infected root segment is not less than 20cm / g soil. At the same time, a treatment group of mixed fungi in which each of the three fungi is uniformly mixed in a mass ratio of 1:1:1 is set, and a treatment group without adding the arbuscular mycorrhizal fungi is set as a control.

[0052] A potted experiment was conducted with 1 kg of mixed soil (containing ammonium molybdate (100 mg / kg) and polyvinyl chloride (1.2% w / w)) inoculated with 10 wt% of the bacterial agent in each pot, with 4 replicates. Six wheat seeds were sown in each pot, and the seedlings were thinned to 2 after one week of germination. The plants were cultured in a greenhouse, and the culture conditions in the greenhouse were ensured to be cultured for 14 hours under the conditions of 10,000 lux and 30°C per day, and cultured for 10 hours under the conditions of 20°C in the dark. During the growth process, water and other treatments were carried out as needed to ensure the normal growth of the plants. After harvesting, indicators such as plant height and yield were measured. The specific experimental groups and results are shown in Table 1.

[0053] Table 1 Plant height and grain yield of wheat (average value)

[0054]

[0055] As shown in Table 1, the treatments with the application of the microbial agent have better plant growth effects than the control group without the application of the microbial agent. The effects of the young glomerulonephritis, the Moses glomerulonephritis and the root cystic ...

[0056] The calculation formula for the total number of reproductive bodies is: total number of reproductive bodies = Σ number of spores + 2 × mycelium density + infected root segments.

[0057] The spore counts, extraradical hyphae, infected root segments and total number of propagules in each treatment group are shown in Table 3.

[0058] Table 2 Propagation number ratio

[0059]

[0060]

[0061] In other implementation situations, the ratio of the number of propagules in the young set of spheromone fungi, the root spheromone fungi, and the Moses spheromone fungi is (1-1.1): (0.7-0.9): (1.3-1.4), which can achieve the same or similar test results as those in the test group E; the ratio of the number of propagules in the young set of spheromone fungi, the Moses spheromone fungi, and the branched giant spheromone fungi is (0.9-1.1):

[0062] (1.2~1.3):(0.8~1.0) can achieve the same or similar experimental results as the experimental group G.

[0063] Table 3 Total number of propagules

[0064]

[0065] Example 3 Preparation of a microbial preparation containing arbuscular mycorrhizal fungi

[0066] The preparation process of the microbial preparation containing arbuscular mycorrhizal fungi in this embodiment is as follows:

[0067] On the basis of selecting the mixed arbuscular mycorrhizal fungi agent with better effect in Example 2 (experimental group E and experimental group G), the treatment of adding beneficial bacteria and synergist was set, and the formula of each experimental group was as follows (by mass ratio):

[0068] Test group I: 85% of the mixed arbuscular mycorrhizal fungi agent of test group E, 5% of Bacillus subtilis, 5% of Trichoderma longifolia, 2% of polyglutamic acid, 2% of humic acid, and 0.04% of ammonium molybdate;

[0069] Test group J: 85% of the mixed arbuscular mycorrhizal fungi agent of test group E, 5% of Bacillus licheniformis, 5% of Trichoderma longibrachiatum, 2% of potassium fulvic acid, 2% of humic acid, and 0.04% of ammonium molybdate;

[0070] Test group K: 85% of the mixed arbuscular mycorrhizal fungi agent of test group E, 5% of Bacillus licheniformis, 5% of Bacillus megaterium, 2% of potassium fulvic acid, 2% of alginic acid, and 0.04% of ammonium molybdate;

[0071] Test group L: 85% of the mixed arbuscular mycorrhizal fungi agent of test group G, 5% of Bacillus subtilis, 5% of Trichoderma longibrachiatum, 2% of polyglutamic acid, 2% of humic acid, and 0.04% of ammonium molybdate;

[0072] Test group M: 85% of the mixed arbuscular mycorrhizal fungi agent of test group G, 5% of Bacillus licheniformis, 5% of Trichoderma longibrachiatum, 2% of potassium fulvic acid, 2% of humic acid, and 0.04% of ammonium molybdate;

[0073] Test group N: 85% of the mixed arbuscular mycorrhizal fungi agent of test group G, 5% of Bacillus licheniformis, 5% of Bacillus megaterium, 2% of potassium humate, 2% of alginic acid, and 0.04% of ammonium molybdate.

[0074] Note: Bacillus subtilis is a liquid bacterial agent with an effective viable bacterial count of 4×10 8 ~4×10 9 CFU / g; Trichoderma longifolia is a solid powder agent, and the effective viable count concentration is 3×10 8 ~3×10 9 CFU / g; Bacillus licheniformis and Bacillus megaterium are solid powder inoculants, and the effective viable bacterial count concentration of Bacillus licheniformis is 2×10 9 ~2×10 10 CFU / g, the effective viable bacterial count of Bacillus megaterium is 2×10 8 ~5×10 8 CFU / g.

[0075] A potted experiment was conducted with 1 kg of ammonium molybdate (100 mg / kg), polyvinyl chloride (1.2% w / w) and 10 wt% of the above-mentioned microbial preparation in each pot, with 4 replicates. Six wheat seeds were sown in each pot, and thinned to 2 after one week of germination. Cultivate in a greenhouse, and ensure that the greenhouse culture conditions are 10000 lux and 30 ° C for 14 hours a day, and 20 ° C in the dark for 10 hours. During the growth process, water treatment and other treatments were carried out as needed to ensure normal growth of the plants. After harvesting, indicators such as plant height and yield were measured. The specific experimental results are shown in Table 4.

[0076] Table 4 Plant height and yield of wheat

[0077]

[0078] As shown in Table 4, the growth effect of wheat treated with Bacillus subtilis, Trichoderma longibrachiatum, polyglutamic acid, humic acid and ammonium molybdate in the arbuscular mycorrhizal fungi agent was better than that of the treatment with only arbuscular mycorrhizal fungi agent and the treatment with other beneficial bacteria and enhancers.

[0079] The results of the correlation analysis of yield are shown in Table 5, which shows that the effect of the mixed inoculum of Glomus juvenile, Glomus mossea and Rhizocystis endoradicata is mainly correlated with Glomus juvenile and Glomus mossea, and the correlation of the treatment with beneficial bacteria and auxiliary materials added on their basis is the best. The effect of the mixed inoculum of Glomus juvenile, Glomus mossea and Rhizocystis endoradicata is most correlated with Glomus mossea, and the correlation of the treatment with beneficial bacteria and auxiliary materials added on their basis is the best.

[0080] Table 5 Correlation analysis

[0081]

[0082] 2. Specific examples of the use of a microbial preparation in promoting plant growth and development in soils that are dually contaminated by microplastics and molybdenum:

[0083] Example 4 Application of microbial preparations in soil contaminated with microplastics and molybdenum 1

[0084] In this embodiment, the young set of near-clear spherical mold agent, root intraradical spherical mold agent and Moses spherical mold agent prepared in Example 1 and the young set of near-clear spherical mold agent, Moses spherical mold agent and branch giant spherical mold agent are mixed in a mass ratio of 1:1:1 to obtain mixed arbuscular mycorrhizal fungi agents. According to the mass ratio, the formula of mixed arbuscular mycorrhizal fungi agent 85%, Bacillus subtilis 5%, long-branched Trichoderma 5%, polyglutamic acid 2%, humic acid 2%, and ammonium molybdate 0.04% is weighed and mixed to obtain microbial preparations AM1 (experimental group I) and AM2 (experimental group L) containing arbuscular mycorrhizal fungi. The total number of propagules in AM1 is 122, and the specific data are shown in Table 3 for test group E, and the total number of propagules in AM2 is 121, and the specific data are shown in Table 3 for test group G.

[0085] Ammonium molybdate and polyvinyl chloride were added to the farmland soil after passing through a 2mm sieve, and then the microbial preparation containing arbuscular mycorrhizal fungi was added to the soil mixed with ammonium molybdate (100mg / kg) and polyvinyl chloride (1.2%w / w) at an inoculation rate of 10wt% for mixing. In addition, a treatment without adding the microbial preparation containing arbuscular mycorrhizal fungi was set up. A potted experiment was carried out with 1kg of mixed soil in each pot, and 4 replicates were set up. Six mung bean seeds were sown in each pot, and the seedlings were thinned into 2 plants after one week of germination. Put them in the greenhouse for cultivation, and ensure that the cultivation conditions of the greenhouse are cultivated for 14 hours under the conditions of 10000lux and 30℃ every day, and cultivated for 10 hours under the conditions of 20℃ in the dark. During the growth process, water treatment and other treatments were carried out as needed to ensure the normal growth of mung beans. After harvesting, indicators such as plant height, yield, and chlorophyll content were measured.

[0086] Specific results such as Figure 1 As shown in the figure, it can be seen that under the conditions of mixed pollution of microplastics and molybdenum, the plant height of mung beans inoculated with microbial preparations containing arbuscular mycorrhizal fungi in the potted experiment was 23.2 cm, and the plant height of mung beans without inoculation was 11.8 cm. Inoculation with microbial preparations containing arbuscular mycorrhizal fungi also increased the P content and grain yield of mung bean grains by 1.1 and 1.5 times, respectively.

[0087] Example 5 Application of microbial preparations in soil contaminated with microplastics and molybdenum 2

[0088] In this embodiment, the young set of glomerulonephritis, root endocystis and Moses glomerulonephritis prepared in Example 1 and the young set of glomerulonephritis, Moses glomerulonephritis and branch giant cystis are mixed uniformly in a mass ratio of 1:1:1 to obtain mixed arbuscular mycorrhizal fungi agents. According to the formula of 85% mixed arbuscular mycorrhizal fungi agent, 5% Bacillus subtilis, 5% long-branched Trichoderma, 2% polyglutamic acid, 2% humic acid, and 0.04% ammonium molybdate, the microbial preparations AM1 (experimental group I) and AM2 (experimental group L) containing arbuscular mycorrhizal fungi are obtained after being weighed and mixed uniformly. The total number of propagules in AM1 is 122, and the specific data are shown in Table 3 for test group E, and the total number of propagules in AM2 is 121, and the specific data are shown in Table 3 for test group G.

[0089] Polyvinyl chloride was added to the farmland soil after passing through a 2mm sieve, and then the microbial preparation containing arbuscular mycorrhizal fungi was added to the soil mixed with ammonium molybdate (100mg / kg) and polyvinyl chloride (1.2%w / w) at an inoculation rate of 10wt% for mixing. In addition, a treatment without adding the microbial preparation containing arbuscular mycorrhizal fungi was set up. A potted experiment was carried out with 1kg of mixed soil in each pot, and 4 replicates were set up. Six corn seeds were sown in each pot, and the seedlings were thinned into 2 plants after one week of germination. Put them in the greenhouse for cultivation, and ensure that the cultivation conditions of the greenhouse are cultivated for 14 hours under the conditions of 10000lux and 30℃ every day, and cultivated for 10 hours under the conditions of 20℃ in the dark. During the growth process, water treatment and other treatments were carried out as needed to ensure the normal growth of corn. After harvest, indicators such as plant height, yield, and chlorophyll content were measured.

[0090] Specific results such as Figure 2 As shown in the figure, it can be seen that for corn potted plants, inoculation with arbuscular mycorrhizal fungi-containing microbial preparations increased corn plant height, chlorophyll content and yield by 1.2, 1.5 and 2.2 times, respectively.

[0091] Example 6 Application of microbial preparations in soil contaminated with microplastics and molybdenum

[0092] In this embodiment, the young set of glomerulonephritis, root endocystis and Moses glomerulonephritis prepared in Example 1 and the young set of glomerulonephritis, Moses glomerulonephritis and branch giant cystis are mixed uniformly in a mass ratio of 1:1:1 to obtain mixed arbuscular mycorrhizal fungi agents. According to the formula of 85% mixed arbuscular mycorrhizal fungi agent, 5% Bacillus subtilis, 5% long-branched Trichoderma, 2% polyglutamic acid, 2% humic acid, and 0.04% ammonium molybdate, the microbial preparations AM1 (experimental group I) and AM2 (experimental group L) containing arbuscular mycorrhizal fungi are obtained after being weighed and mixed uniformly. The total number of propagules in AM1 is 122, and the specific data are shown in Table 3 for test group E, and the total number of propagules in AM2 is 121, and the specific data are shown in Table 3 for test group G.

[0093] Ammonium molybdate was added to the farmland soil after passing through a 2mm sieve, and then the microbial preparation of arbuscular mycorrhizal fungi was added to the soil mixed with ammonium molybdate (100mg / kg) and polyvinyl chloride (1.2%w / w) at an inoculation rate of 10wt% and mixed. In addition, a treatment without adding the microbial preparation containing arbuscular mycorrhizal fungi was set up. A potted experiment was carried out with 1kg of mixed soil in each pot, and 4 replicates were set up. Six wheat seeds were sown in each pot, and the seedlings were thinned into one after one week of germination. Put it in the greenhouse for cultivation, and ensure that the cultivation conditions of the greenhouse are cultivated for 14h under the conditions of 10000lux and 30℃ every day, and cultivated for 10h under the conditions of 20℃ in the dark. During the growth process, water treatment and other treatments were carried out as needed to ensure the normal growth of wheat. After harvest, indicators such as plant height, yield, and chlorophyll content were measured.

[0094] Specific results such as Figure 3 As shown in the figure, it can be seen that for wheat potted plants, inoculation with arbuscular mycorrhizal fungi-containing microbial preparations increased wheat plant height, chlorophyll content and grain yield by 1.4, 1.3 and 1.4 times, respectively.

[0095] Example 7 Field Experiment

[0096] In this embodiment, the young set of glomerulonephritis, root endocystis and Moses glomerulonephritis prepared in Example 1 and the young set of glomerulonephritis, Moses glomerulonephritis and branch giant cystis are mixed uniformly in a mass ratio of 1:1:1 to obtain mixed arbuscular mycorrhizal fungi agents. According to the formula of 85% mixed arbuscular mycorrhizal fungi agent, 5% Bacillus subtilis, 5% long-branched Trichoderma, 2% polyglutamic acid, 2% humic acid, and 0.04% ammonium molybdate, the microbial preparations AM1 (experimental group I) and AM2 (experimental group L) containing arbuscular mycorrhizal fungi are obtained after being weighed and mixed uniformly. The total number of propagules in AM1 is 122, and the specific data are shown in Table 3 for test group E, and the total number of propagules in AM2 is 121, and the specific data are shown in Table 3 for test group G.

[0097] Field experiments were conducted in farmland around the molybdenum mining area in Luanchuan County, Luoyang City, where agricultural plastic film has been used for a long time. The molybdenum content was 360.84 mg / kg and the abundance of microplastics was 894 pieces / kg. The test site was completely randomly divided into 6 test plots, each with an area of ​​40m 2(5m×8m), with 2 treatments and 3 repetitions. Plant 10 rows in each plot, with a row spacing of 0.5m, each row length of 8m, plant spacing of 20cm, 40 plants in each row, and a density of 400 plants in each plot, with a walkway of 2m between plots. Sow 5 corn seeds in each hole. Apply 100 grams of microbial preparations containing arbuscular mycorrhizal fungi to each hole in the inoculation treatment, cover the seeds, and cover with soil. Cover the soil directly without inoculation treatment. Thin out the corn seedlings when they are 10cm tall, leaving 1 seed in each hole. Carry out field management measures such as weeding, pest control, and watering during the growth of corn. Harvest and measure indicators when the corn is mature.

[0098] Specific results such as Figure 4 As shown in the figure, it can be seen that the field experiment inoculation with arbuscular mycorrhizal fungi microbial preparations increased corn yield by 1.1 times.

[0099] The above description is only an illustrative embodiment of the present invention and is not intended to limit the scope of the present invention. Any equivalent changes and modifications made by a person of ordinary skill in the art without departing from the concept and principle of the present invention shall fall within the scope of protection of the present invention.

Claims

1. A microbial preparation containing arbuscular mycorrhizal fungi, characterized in that: The microbial preparation includes arbuscular mycorrhizal fungal agents; the arbuscular mycorrhizal fungal agents are young globus sac mold agents, root endocystic cyst mold agents and Moses globus sac mold agents or young globus sac mold agents, Moses globus sac mold agents and branched giant cyst mold agents; the reproductive body ratio of the young globus sac mold agents, root endocystic cyst mold agents and Moses globus sac mold agents is (1-1.1): (0.7-0.9): (1.3-1.4); the reproductive body ratio of the young globus sac mold agents, Moses globus sac mold agents and branched giant cyst mold agents is (0.9-1.1): (1.2-1.3): (0.8-1.0).

2. The microbial preparation containing arbuscular mycorrhizal fungi according to claim 1, characterized in that: The number of propagules in the arbuscular mycorrhizal fungal agent is not less than 110 per gram.

3. The microbial preparation containing arbuscular mycorrhizal fungi according to claim 1 or 2, characterized in that: The propagules are composed of spores, extraradical hyphae and infected root segments.

4. The microbial preparation containing arbuscular mycorrhizal fungi according to claim 3, characterized in that: The young set of nearly bright spore mold agent, root root spore mold agent, Moses spore mold agent and branch giant spore mold agent meet the following standards: the number of spores is not less than 6 / gram, the extra-root mycelium density is not less than 18 m / g, and the infected root segment is not less than 20 cm / g.

5. The microbial preparation containing arbuscular mycorrhizal fungi according to claim 4, characterized in that: The microbial preparation comprises bacillus subtilis, trichoderma longifolia, polyglutamic acid, humic acid and ammonium molybdate.

6. The microbial preparation containing arbuscular mycorrhizal fungi according to claim 5, characterized in that: The mass ratio of the arbuscular mycorrhizal fungal agent, Bacillus subtilis, Trichoderma longifolia, polyglutamic acid, humic acid and ammonium molybdate is 85: (4-5): (4-5): (2-3): (2-3): (0.04-0.05).

7. The microbial preparation containing arbuscular mycorrhizal fungi according to claim 6, characterized in that: The effective viable count of Bacillus subtilis is not less than 4×10 8 CFU / g, the effective viable count of Trichoderma longifolia is not less than 8×10 8 CFU / g.

8. Use of the microbial preparation as described in any one of claims 1 to 7 in promoting plant growth and development in soil doubly contaminated by microplastics and molybdenum.

9. The use of the microbial preparation according to claim 8 in promoting plant growth and development in soils that are dually contaminated by microplastics and molybdenum, characterized in that: The growth and development refers to plant height, yield and / or chlorophyll content.

10. Use of the microbial preparation according to claim 8 or 9 in promoting plant growth and development in soils that are contaminated by microplastics and molybdenum, characterized in that: The plants include wheat, mung bean and corn.

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