A method for preventing and controlling vegetable seedling pith necrosis disease

Abstract

The present application relates to the technical field of agricultural pest control, and specifically discloses a method for preventing and controlling vegetable seedling pith necrosis. The method for preventing and controlling vegetable seedling pith necrosis refers to diluting the biological agent and ethionine with water at a mass ratio of 7-9:1:8000-10000 and spraying them on the surface of the vegetable seedling plants. The biological agent is composed of the following raw materials in parts by weight: 18-22 parts of Coptis chinensis, 3-5 parts of Honeysuckle, 3-5 parts of Forsythia suspensa, 1-3 parts of Portulaca oleracea, 4-6 parts of Taraxacum officinale, and 2-4 parts of Sophora flavescens. The vegetable seedling pith necrosis prevention and control method of the present application is simple to operate, natural and pollution-free, has a significant bactericidal effect, and greatly improves the quality of seedlings and reduces environmental hazards.

Description

Technical Field

[0001] The present application relates to the technical field of agricultural pest control, and more specifically, to a method for controlling vegetable seedling pith necrosis. Background Art

[0002] Bacterial pith necrosis is caused by Pseudomonas pith necrosis, and the main symptoms are necrosis and rot of the pith of the plant. In severe cases, the bacteria will invade the stems and leaves, causing the death of the entire plant. The disease spreads quickly and is extremely harmful, with an average disease rate of more than 10%, and severe cases as high as 30-50%, causing great losses to plant production.

[0003] At present, the main methods for preventing and controlling bacterial pith necrosis of vegetables are to use chemical agents and strengthen agricultural management measures. Although these methods have achieved certain prevention and control effects, the prevention and control rate is very low. In addition, the use of excessive chemical agents, such as 3000-4000 times of 72% agricultural streptomycin soluble powder, 4000 times of 90% phytosan soluble powder, or 500 times of 77% copper hydroxide wettable powder, will not only cause pathogens to develop drug resistance, but many chemical pesticides that are not easily degraded will also be enriched in vegetables, affecting product quality and polluting the air, soil and other environments. With the improvement of living standards, people have higher and higher requirements for green and healthy products. Therefore, it is urgent to provide an efficient and safe method for preventing and controlling pith necrosis of vegetable seedlings. Summary of the invention

[0004] In order to solve the problems in the related art that the chemical control rate of vegetable bacterial pith necrosis is low, it is easy to develop drug resistance, and it pollutes plants and the environment, the present application provides a method for preventing and controlling vegetable seedling pith necrosis.

[0005] The present application provides a method for preventing and treating vegetable seedling pith necrosis, which adopts the following technical scheme:

[0006] A method for preventing and treating vegetable seedling pith necrosis disease comprises the following steps:

[0007] (1) By weight, 18-22 parts of Coptis chinensis, 3-5 parts of Honeysuckle, 3-5 parts of Forsythia suspensa, 1-3 parts of Portulaca oleracea, 4-6 parts of Taraxacum officinale, and 2-4 parts of Sophora flavescens are mixed to prepare a biological agent;

[0008] (2) Mix the biological agent and ethionine with water in a mass ratio of 7-9:1:8000-10000 to obtain a diluted agent;

[0009] (3) Spray the diluted agent onto the surface of vegetable seedlings.

[0010] Preferably, the biological agent in step (1) comprises the following raw materials in parts by weight: 20 parts of coptis root, 4 parts of honeysuckle, 4 parts of forsythia, 2 parts of purslane, 5 parts of dandelion, and 3 parts of sophora flavescens.

[0011] Preferably, in step (2), the mass ratio of the biological agent, ethionine and water is 8:1:9000.

[0012] Preferably, the method for preparing the biological agent in step (1) comprises the following steps:

[0013] S1. Mix and grind coptis root, honeysuckle, forsythia, purslane, dandelion and sophora flavescens by weight and pass through a 60-100 mesh sieve to obtain a mixed powder;

[0014] S2, adding water to the mixed powder at a mass ratio of 1:8-12 for boiling extraction, cooling to 32-40°C to obtain a water extract, adding composite microorganisms to the water extract for sealed fermentation to obtain a fermentation liquid;

[0015] S3. After adding the mixed salt solution to the fermentation liquid and blending evenly, the mixture is further sealed for fermentation, and the fermentation product is centrifuged and dried to obtain a biological agent.

[0016] Preferably, the composite microorganism in step S2 consists of Bacillus amyloliquefaciens, Bacillus mucilaginosus and Trichoderma with a live cell count ratio of 4-6:3-5:1.

[0017] Preferably, the viable bacterial count of the composite microorganisms in the water extract in step S2 is 1.8-2.2×10 9 CFU / ml, fermentation time is 24-48h.

[0018] Preferably, the mixed salt solution in step S3 is a mixture of magnesium succinate, copper sulfate and deionized water in a mass ratio of 4-6:2-4:100.

[0019] Preferably, in step S3, the mass ratio of the mixed salt solution to the fermentation liquid is 4-8:100.

[0020] Preferably, in step S3, the fermentation temperature is 32-40° C., and the fermentation time is 48-72 h.

[0021] Preferably, the centrifugal speed is 8000-10000 r / min, the centrifugal time is 10-18 min, the drying temperature is 50-58° C., and the drying time is 6-8 h.

[0022] In summary, this application has the following beneficial effects:

[0023] 1. This application uses a variety of Chinese medicinal materials including coptis root, honeysuckle, forsythia, purslane, dandelion, and sophora flavescens. Among them, coptis root contains berberine, which can effectively inhibit the growth of Pseudomonas medullary necrosis; honeysuckle, forsythia, purslane and sophora flavescens also have a spectrum of bactericidal effects. These Chinese medicinal materials are rich in nutrients, rich in active ingredients such as various vitamins, minerals, and proteins. They are pure natural components, non-toxic and pollution-free. Mixing a variety of Chinese medicinal materials broadens the antibacterial range and synergistically enhances the antibacterial ability.

[0024] 2. The present application adopts a mixture of multiple Chinese medicinal materials as fermentation raw materials, and adds composite microorganisms and mixed salt solutions to the fermentation raw materials in sequence for fermentation. Multiple fermentations not only promote the Chinese medicinal materials to release more active substances and enhance the antibacterial ability, but also greatly improve the activity, resistance and adaptability of microorganisms; the combined use of Bacillus amyloliquefaciens, Bacillus mucilaginosus and Trichoderma can enhance each other's synergy and produce more biologically active substances that have antagonistic effects on pathogens, promote plant growth, and enhance plant immunity, thereby improving the prevention and control effect.

[0025] 3. The present application adopts a mixed solution of magnesium succinate, copper sulfate and deionized water to prepare a mixed salt solution. By adding the mixed salt solution during the fermentation process, firstly, it can promote fermentation, provide additional nutrients for microorganisms, promote the growth and metabolic activities of microorganisms, thereby increasing the yield of useful products in the fermentation process and improving the fermentation efficiency; secondly, it can regulate the osmotic effect of microbial cell membranes, increase the activity of microorganisms, and thus increase the utilization efficiency of nutrients by microorganisms; thirdly, it can increase the ductility of microbial cell walls, making it easier to release enzymes and metabolites in the cells, thereby increasing the fermentation rate and fermentation effect, and improving the yield and quality of fermentation products.

[0026] 4. The biological agent for preventing and controlling vegetable seedling pith necrosis disease used in this application is a green and pollution-free control agent that is easily degradable and has little harm to the environment. It also has a significant bactericidal effect and can effectively improve product quality and yield. DETAILED DESCRIPTION

[0027] The present application is further described in detail below with reference to the embodiments.

[0028] Embodiment 1-5 provides a method for preventing and treating vegetable seedling pith necrosis.

[0029] Example 1

[0030] A method for preventing and treating vegetable seedling pith necrosis disease comprises the following steps:

[0031] (1) Mix coptis root, honeysuckle, forsythia, purslane, dandelion and sophora flavescens to prepare a biological agent;

[0032] (2) Add water to the biological agent and ethionine in a mass ratio of 7:1:8000 and mix well to obtain a diluted agent;

[0033] (3) Spray the diluted pesticide onto the surface of vegetable seedlings and control the dosage to 50L / mu.

[0034] The biological agent is prepared by the following method:

[0035] S1. By weight, 18 parts of Coptis chinensis, 3 parts of Honeysuckle, 3 parts of Forsythia suspensa, 1 part of Portulaca oleracea, 4 parts of Taraxacum officinale, and 2 parts of Sophora flavescens were mixed and crushed through a 60-mesh sieve to obtain a mixed powder;

[0036] S2. Add water to the mixed powder at a mass ratio of 1:8 and boil for 15 minutes, then cool to 32°C to obtain a water extract. Add composite microorganisms to the water extract and control the effective viable count of the composite microorganisms in the water extract to 1.8×10 9 CFU / ml, sealed and fermented for 24h to obtain the fermentation liquid;

[0037] S3. Add the mixed salt solution to the fermentation liquid at a mass ratio of 4:100, mix evenly, and then seal and ferment at 32°C for 48 hours. Control the centrifugal speed to 8000 r / min and the centrifugal time to 10 minutes. Centrifuge the fermentation product and dry it at 50°C for 6 hours to obtain a biological agent.

[0038] The composite microorganisms in step S2 are composed of Bacillus amyloliquefaciens, Bacillus mucilaginosus and Trichoderma at a live bacteria ratio of 4:3:1.

[0039] The mixed salt solution in step S3 is a mixed solution of magnesium succinate, copper sulfate and deionized water in a mass ratio of 4:2:100.

[0040] Example 2

[0041] A method for preventing and treating vegetable seedling pith necrosis disease comprises the following steps:

[0042] (1) Mix coptis root, honeysuckle, forsythia, purslane, dandelion and sophora flavescens to prepare a biological agent;

[0043] (2) Add water to the biological agent and ethionine in a mass ratio of 7.5:1:8500 and mix well to obtain a diluted agent;

[0044] (3) Spray the diluted pesticide onto the surface of vegetable seedlings and control the dosage to 51L / mu.

[0045] The biological agent is prepared by the following method:

[0046] S1. By weight, 19 parts of Coptis chinensis, 3.5 parts of Honeysuckle, 3.5 parts of Forsythia suspensa, 1.5 parts of Portulaca oleracea, 4.5 parts of Taraxacum officinale, and 2.5 parts of Sophora flavescens were mixed and crushed through a 70-mesh sieve to obtain a mixed powder;

[0047] S2. Add water to the mixed powder at a mass ratio of 1:9 and boil for 15 minutes, then cool to 34°C to obtain a water extract. Add composite microorganisms to the water extract and control the effective viable count of the composite microorganisms in the water extract to 1.9×10 9 CFU / ml, sealed and fermented for 30h to obtain the fermentation liquid;

[0048] S3. Add the mixed salt solution to the fermentation liquid at a mass ratio of 5:100, mix evenly, and then further seal and ferment at 34°C for 54 hours. Control the centrifugal speed to 8500 r / min and the centrifugal time to 12 minutes. Centrifuge the fermentation product and dry it at 52°C for 6.5 hours to obtain a biological agent.

[0049] The composite microorganisms in step S2 are composed of Bacillus amyloliquefaciens, Bacillus mucilaginosus and Trichoderma at a live bacteria count ratio of 4.5:3.5:1.

[0050] The mixed salt solution in step S3 is a mixture of magnesium succinate, copper sulfate and deionized water in a mass ratio of 4.5:2.5:100.

[0051] Example 3

[0052] A method for preventing and treating vegetable seedling pith necrosis disease comprises the following steps:

[0053] (1) Mix coptis root, honeysuckle, forsythia, purslane, dandelion and sophora flavescens to prepare a biological agent;

[0054] (2) Add water to the biological agent and ethionine in a mass ratio of 8:1:9000 and mix well to obtain a diluted agent;

[0055] (3) Spray the diluted pesticide onto the surface of vegetable seedlings and control the dosage to 52L / mu.

[0056] The biological agent is prepared by the following method:

[0057] S1. Mix 20 parts of Coptis chinensis, 4 parts of Honeysuckle, 4 parts of Forsythia suspensa, 2 parts of Portulaca oleracea, 5 parts of Taraxacum officinale, and 3 parts of Sophora flavescens, and grind them through an 80-mesh sieve to obtain a mixed powder;

[0058] S2. Add water to the mixed powder at a mass ratio of 1:10 and boil for 15 minutes, then cool to 36°C to obtain a water extract. Add composite microorganisms to the water extract and control the effective viable count of the composite microorganisms to 2.0×10 9CFU / ml, sealed and fermented for 36h to obtain the fermentation liquid;

[0059] S3. Add the mixed salt solution to the fermentation liquid at a mass ratio of 6:100, mix well, and then seal and ferment at 36°C for 60 hours. Control the centrifugal speed to 9000 r / min and the centrifugal time to 14 minutes. Centrifuge the fermentation product and dry it at 54°C for 7 hours to obtain a biological agent.

[0060] The composite microorganisms in step S3 are composed of Bacillus amyloliquefaciens, Bacillus mucilaginosus and Trichoderma at a live bacteria ratio of 5:4:1.

[0061] The mixed salt solution in step S2 is a mixed solution of magnesium succinate, copper sulfate and deionized water in a mass ratio of 5:3:100.

[0062] Example 4

[0063] A method for preventing and treating vegetable seedling pith necrosis disease comprises the following steps:

[0064] (1) Mix coptis root, honeysuckle, forsythia, purslane, dandelion and sophora flavescens to prepare a biological agent;

[0065] (2) Add water to the biological agent and ethionine in a mass ratio of 8.5:1:9500 and mix well to obtain a diluted agent;

[0066] (3) Spray the diluted pesticide onto the surface of vegetable seedlings and control the dosage to 53L / mu.

[0067] The biological agent is prepared by the following method:

[0068] S1. By weight, 21 parts of Coptis chinensis, 4.5 parts of Honeysuckle, 4.5 parts of Forsythia suspensa, 2.5 parts of Portulaca oleracea, 5.5 parts of Taraxacum officinale, and 3.5 parts of Sophora flavescens were mixed and crushed through a 90-mesh sieve to obtain a mixed powder;

[0069] S2. Add water to the mixed powder at a mass ratio of 1:11 and boil for 15 minutes, then cool to 38°C to obtain a water extract. Add composite microorganisms to the water extract and control the effective viable count of the composite microorganisms to 2.1×10 9 CFU / ml, sealed and fermented for 42h to obtain the fermentation liquid;

[0070] S3. Add the mixed salt solution to the fermentation liquid at a mass ratio of 7:100, mix evenly, and then seal and ferment at 38°C for 66 hours. Control the centrifugal speed to 9500 r / min and the centrifugal time to 16 minutes. Centrifuge the fermentation product and dry it at 56°C for 7.5 hours to obtain a biological agent.

[0071] The composite microorganisms in step S3 are composed of Bacillus amyloliquefaciens, Bacillus mucilaginosus and Trichoderma at a live bacteria count ratio of 5.5:4.5:1.

[0072] The mixed salt solution in step S2 is a mixed solution of magnesium succinate, copper sulfate and deionized water in a mass ratio of 5.5:3.5:100.

[0073] Example 5

[0074] (1) Mix coptis root, honeysuckle, forsythia, purslane, dandelion and sophora flavescens to prepare a biological agent;

[0075] (2) Add water to the biological agent and ethionine in a mass ratio of 9:1:10000 and mix well to obtain a diluted agent;

[0076] (3) Spray the diluted pesticide onto the surface of vegetable seedlings and control the dosage to 54L / mu.

[0077] The biological agent is prepared by the following method:

[0078] S1. Mix 22 parts of Coptis chinensis, 5 parts of Honeysuckle, 5 parts of Forsythia suspensa, 3 parts of Portulaca oleracea, 6 parts of Taraxacum officinale, and 4 parts of Sophora flavescens, and grind them through a 100-mesh sieve to obtain a mixed powder;

[0079] S2. Add water to the mixed powder at a mass ratio of 1:12 and boil for 15 minutes, then cool to 40°C to obtain a water extract. Add composite microorganisms to the water extract and control the effective viable count of the composite microorganisms to 2.2×10 9 CFU / ml, sealed and fermented for 48h to obtain the fermentation liquid;

[0080] S3. Add the mixed salt solution to the fermentation liquid at a mass ratio of 8:100, mix evenly, and then seal and ferment at 40°C for 72 hours. Control the centrifugal speed to 10,000 r / min and the centrifugal time to 18 minutes. Centrifuge the fermentation product and dry it at 58°C for 8 hours to obtain a biological agent.

[0081] The composite microorganisms in step S3 are composed of Bacillus amyloliquefaciens, Bacillus mucilaginosus and Trichoderma with a live cell count ratio of 6:5:1.

[0082] The mixed salt solution in step S2 is a mixed solution of magnesium succinate, copper sulfate and deionized water in a mass ratio of 6:4:100.

[0083] In order to verify the comprehensive performance of the biological agent for preventing and treating vegetable seedling pith necrosis disease prepared in Examples 1-5 of the present application, the applicant set up comparative examples 1-7, which are as follows:

[0084] Comparative Example 1

[0085] A method for preventing and treating vegetable seedling pith necrosis disease comprises the following steps:

[0086] (1) Mix honeysuckle, forsythia, purslane, dandelion and sophora flavescens to prepare a biological agent;

[0087] (2) Add water to the biological agent and ethionine in a mass ratio of 7:1:8000 and mix well to obtain a diluted agent;

[0088] (3) Spray the diluted pesticide onto the surface of vegetable seedlings and control the dosage to 50L / mu.

[0089] The biological agent is prepared by the following method:

[0090] S1. Mix and grind 3 parts of honeysuckle, 3 parts of forsythia, 1 part of purslane, 4 parts of dandelion and 2 parts of sophora flavescens by weight, and pass through a 60-mesh sieve to obtain a mixed powder;

[0091] S2. Add water to the mixed powder at a mass ratio of 1:8 and boil for 15 minutes, then cool to 32°C to obtain a water extract. Add composite microorganisms to the water extract and control the effective viable count of the composite microorganisms to 1.8×10 9 CFU / ml, sealed and fermented for 24h to obtain the fermentation liquid;

[0092] S3. Add the mixed salt solution to the fermentation liquid at a mass ratio of 4:100, mix evenly, and then seal and ferment at 32°C for 48 hours. Control the centrifugal speed to 8000 r / min and the centrifugal time to 10 minutes. Centrifuge the fermentation product and dry it at 50°C for 6 hours to obtain a biological agent.

[0093] The composite microorganisms in step S2 are composed of Bacillus amyloliquefaciens, Bacillus mucilaginosus and Trichoderma at a live bacteria ratio of 4:3:1.

[0094] The mixed salt solution in step S3 is a mixed solution of magnesium succinate, copper sulfate and deionized water in a mass ratio of 4:2:100.

[0095] Comparative Example 2

[0096] A method for preventing and treating vegetable seedling pith necrosis disease comprises the following steps:

[0097] (1) Mix coptis root, honeysuckle, forsythia, purslane, dandelion and sophora flavescens to prepare a biological agent;

[0098] (2) Add water to the biological agent and ethionine in a mass ratio of 7:1:8000 and mix well to obtain a diluted agent;

[0099] (3) Spray the diluted pesticide onto the surface of vegetable seedlings and control the dosage to 50L / mu.

[0100] The biological agent is prepared by the following method:

[0101] S1. By weight, 18 parts of Coptis chinensis, 3 parts of Honeysuckle, 3 parts of Forsythia suspensa, 1 part of Portulaca oleracea, 4 parts of Taraxacum officinale, and 2 parts of Sophora flavescens were mixed and crushed through a 60-mesh sieve to obtain a mixed powder;

[0102] S2. Add water to the mixed powder at a mass ratio of 1:8 and boil for 15 minutes, then cool to 32°C to obtain a water extract. Add composite microorganisms to the water extract and control the effective viable count of the composite microorganisms to 1.8×10 9 CFU / ml, sealed and fermented for 24h to obtain the fermentation liquid;

[0103] S3. Add the mixed salt solution to the fermentation liquid at a mass ratio of 4:100, mix evenly, and then seal and ferment at 32°C for 48 hours. Control the centrifugal speed to 8000 r / min and the centrifugal time to 10 minutes. Centrifuge the fermentation product and dry it at 50°C for 6 hours to obtain a biological agent.

[0104] The composite microorganisms in step S2 are composed of Bacillus subtilis and Trichoderma at a live bacteria ratio of 7:1.

[0105] The mixed salt solution in step S3 is a mixed solution of magnesium succinate, copper sulfate and deionized water in a mass ratio of 4:2:100.

[0106] Comparative Example 3

[0107] A method for preventing and treating vegetable seedling pith necrosis disease comprises the following steps:

[0108] (1) Mix coptis root, honeysuckle, forsythia, purslane, dandelion and sophora flavescens to prepare a biological agent;

[0109] (2) Add water to the biological agent and ethionine in a mass ratio of 7:1:8000 and mix well to obtain a diluted agent;

[0110] (3) Spray the diluted pesticide onto the surface of vegetable seedlings and control the dosage to 50L / mu.

[0111] The biological agent is prepared by the following method:

[0112] S1. By weight, 18 parts of Coptis chinensis, 3 parts of Honeysuckle, 3 parts of Forsythia suspensa, 1 part of Portulaca oleracea, 4 parts of Taraxacum officinale, and 2 parts of Sophora flavescens were mixed and crushed through a 60-mesh sieve to obtain a mixed powder;

[0113] S2. Add water to the mixed powder at a mass ratio of 1:8 and boil for 15 minutes, then cool to 32°C to obtain a water extract. Add composite microorganisms to the water extract and control the effective viable count of the composite microorganisms to 1.8×10 9 CFU / ml, sealed and fermented for 24h to obtain the fermentation liquid;

[0114] S3. Add the mixed salt solution to the fermentation liquid at a mass ratio of 4:100, mix evenly, and then seal and ferment at 32°C for 48 hours. Control the centrifugal speed to 8000 r / min and the centrifugal time to 10 minutes. Centrifuge the fermentation product and dry it at 50°C for 6 hours to obtain a biological agent.

[0115] The composite microorganism in step S2 is composed of Bacillus amyloliquefaciens and Trichoderma at a live bacteria ratio of 7:1.

[0116] The mixed salt solution in step S3 is a mixed solution of magnesium succinate, copper sulfate and deionized water in a mass ratio of 4:2:100.

[0117] Comparative Example 4

[0118] A method for preventing and treating vegetable seedling pith necrosis disease comprises the following steps:

[0119] (1) Mix coptis root, honeysuckle, forsythia, purslane, dandelion and sophora flavescens to prepare a biological agent;

[0120] (2) Add water to the biological agent and ethionine in a mass ratio of 7:1:8000 and mix well to obtain a diluted agent;

[0121] (3) Spray the diluted pesticide onto the surface of vegetable seedlings and control the dosage to 50L / mu.

[0122] The biological agent is prepared by the following method:

[0123] S1. By weight, 18 parts of Coptis chinensis, 3 parts of Honeysuckle, 3 parts of Forsythia suspensa, 1 part of Portulaca oleracea, 4 parts of Taraxacum officinale, and 2 parts of Sophora flavescens were mixed and crushed through a 60-mesh sieve to obtain a mixed powder;

[0124] S2. Add water to the mixed powder at a mass ratio of 1:8 and boil for 15 minutes, then cool to 32°C to obtain a water extract. Add composite microorganisms to the water extract and control the effective viable count of the composite microorganisms to 1.8×10 9 CFU / ml, sealed and fermented for 24h to obtain the fermentation liquid;

[0125] S3. Add the mixed salt solution to the fermentation liquid at a mass ratio of 4:100, mix evenly, control the centrifugal speed to 8000 r / min, the centrifugal time to 10 min, centrifuge the mixed product and dry it at 50°C for 6 h to obtain a biological agent.

[0126] The composite microorganisms in step S2 are composed of Bacillus amyloliquefaciens, Bacillus mucilaginosus and Trichoderma at a live bacteria ratio of 4:3:1.

[0127] The mixed salt solution in step S3 is a mixed solution of magnesium succinate, copper sulfate and deionized water in a mass ratio of 4:2:100.

[0128] Comparative Example 5

[0129] A method for preventing and treating vegetable seedling pith necrosis disease comprises the following steps:

[0130] (1) Mix coptis root, honeysuckle, forsythia, purslane, dandelion and sophora flavescens to prepare a biological agent;

[0131] (2) Add water to the biological agent and ethionine in a mass ratio of 7:1:8000 and mix well to obtain a diluted agent;

[0132] (3) Spray the diluted pesticide onto the surface of vegetable seedlings and control the dosage to 50L / mu.

[0133] The biological agent is prepared by the following method:

[0134] S1. By weight, 18 parts of Coptis chinensis, 3 parts of Honeysuckle, 3 parts of Forsythia suspensa, 1 part of Portulaca oleracea, 4 parts of Taraxacum officinale, and 2 parts of Sophora flavescens were mixed and crushed through a 60-mesh sieve to obtain a mixed powder;

[0135] S2. Add water to the mixed powder at a mass ratio of 1:8 and boil for 15 minutes, then cool to 32°C to obtain a water extract. Add composite microorganisms to the water extract and control the effective viable count of the composite microorganisms to 1.8×10 9 CFU / ml, sealed and fermented for 24h to obtain the fermentation liquid;

[0136] S3. Add the mixed salt solution to the fermentation liquid at a mass ratio of 4:100, mix evenly, and then seal and ferment at 32°C for 48 hours. Control the centrifugal speed to 8000 r / min and the centrifugal time to 10 minutes. Centrifuge the fermentation product and dry it at 50°C for 6 hours to obtain a biological agent.

[0137] The composite microorganisms in step S2 are composed of Bacillus amyloliquefaciens, Bacillus mucilaginosus and Trichoderma at a live bacteria ratio of 4:3:1.

[0138] The salt solution in step S3 is a mixture of magnesium succinate and deionized water in a mass ratio of 6:100.

[0139] Comparative Example 6

[0140] A method for preventing and treating vegetable seedling pith necrosis disease comprises the following steps:

[0141] (1) Mix coptis root, honeysuckle, forsythia, purslane, dandelion and sophora flavescens to prepare a biological agent;

[0142] (2) Add water to the biological agent and ethionine in a mass ratio of 7:1:8000 and mix well to obtain a diluted agent;

[0143] (3) Spray the diluted pesticide onto the surface of vegetable seedlings and control the dosage to 50L / mu.

[0144] The biological agent is prepared by the following method:

[0145] S1. By weight, 18 parts of Coptis chinensis, 3 parts of Honeysuckle, 3 parts of Forsythia suspensa, 1 part of Portulaca oleracea, 4 parts of Taraxacum officinale, and 2 parts of Sophora flavescens were mixed and crushed through a 60-mesh sieve to obtain a mixed powder;

[0146] S2. Add water to the mixed powder at a mass ratio of 1:8 and boil for 15 minutes, then cool to 32°C to obtain a water extract. Add composite microorganisms to the water extract and control the effective viable count of the composite microorganisms to 1.8×10 9 CFU / ml, sealed and fermented for 24h to obtain the fermentation liquid;

[0147] S3. Add the mixed salt solution to the fermentation liquid at a mass ratio of 4:100, mix evenly, and then seal and ferment at 32°C for 48 hours. Control the centrifugal speed to 8000 r / min and the centrifugal time to 10 minutes. Centrifuge the fermentation product and dry it at 50°C for 6 hours to obtain a biological agent.

[0148] The composite microorganisms in step S2 are composed of Bacillus amyloliquefaciens, Bacillus mucilaginosus and Trichoderma at a live bacteria ratio of 4:3:1.

[0149] The salt solution in step S3 is a mixture of copper sulfate and deionized water in a mass ratio of 6:100.

[0150] Comparative Example 7

[0151] A method for preventing and treating vegetable seedling pith necrosis disease comprises the following steps:

[0152] (1) Mix coptis root, honeysuckle, forsythia, purslane, dandelion and sophora flavescens to prepare a biological agent;

[0153] (2) Add water to the biological agent at a mass ratio of 8:8000 and mix well to obtain a diluted agent;

[0154] (3) Spray the diluted pesticide onto the surface of vegetable seedlings and control the dosage to 50L / mu.

[0155] The biological agent is prepared by the following method:

[0156] S1. By weight, 18 parts of Coptis chinensis, 3 parts of Honeysuckle, 3 parts of Forsythia suspensa, 1 part of Portulaca oleracea, 4 parts of Taraxacum officinale, and 2 parts of Sophora flavescens were mixed and crushed through a 60-mesh sieve to obtain a mixed powder;

[0157] S2. Add water to the mixed powder at a mass ratio of 1:8 and boil for 15 minutes, then cool to 32°C to obtain a water extract. Add composite microorganisms to the water extract and control the effective viable count of the composite microorganisms to 1.8×10 9 CFU / ml, sealed and fermented for 24h to obtain the fermentation liquid;

[0158] S3. Add the mixed salt solution to the fermentation liquid at a mass ratio of 4:100, mix evenly, and then seal and ferment at 32°C for 48 hours. Control the centrifugal speed to 8000 r / min and the centrifugal time to 10 minutes. Centrifuge the fermentation product and dry it at 50°C for 6 hours to obtain a biological agent.

[0159] The composite microorganisms in step S2 are composed of Bacillus amyloliquefaciens, Bacillus mucilaginosus and Trichoderma at a live bacteria ratio of 4:3:1.

[0160] The mixed salt solution in step S3 is a mixed solution of magnesium succinate, copper sulfate and deionized water in a mass ratio of 4:2:100.

[0161] Performance Testing

[0162] The antibacterial effects of the biological agents for preventing and controlling vegetable seedling pith necrosis disease prepared in Examples 1-5 of the present application and Comparative Examples 1-7 were tested respectively.

[0163] 1. In vitro activity assay

[0164] The in-dish plate confrontation method was used to determine the in-dish antagonistic activity of the biological agents prepared in Examples 1-5 and Comparative Examples 1-7 against the pathogens of bacterial pith necrosis (strain 1: Pseudomonas rugosa Bio-02594, provided by Beijing Biobowei Biotechnology Co., Ltd.; strain 2: Pseudomonas chicory SHBCC D50210, provided by Shanghai Xuanke Biotechnology Co., Ltd.). The specific test process is as follows: 1 ml of the diluted agent prepared in Examples 1-5 and Comparative Examples 1-7 was added to a filter paper disc with a diameter of 8 mm to prepare tablets with a certain dose, which were inverted on both sides of the PDA plate, and cultured at 28°C for 2 days. The pathogenic bacteria cakes of strains 1 and 2 were inoculated in the center of the plate, and the treatment of inoculating only the pathogenic bacteria cakes in the center of the plate was used as a control. Each treatment was repeated three times and cultured at a constant temperature of 28°C. When the control colony covered the plate, the diameter of the inhibition zone was observed and recorded, and the inhibition rate was calculated.

[0165] Inhibition rate (%) = (1-antagonistic treatment lesion radius / control lesion radius) × 100%. The measurement results are shown in Table 1.

[0166] Table 1:

[0167]

[0168] It can be seen from Table 1 that the biological agent for preventing and controlling vegetable seedling pith necrosis disease prepared by the present invention has a significant inhibitory effect on strains 1 and 2, and the inhibition rate of Examples 1-5 is significantly higher than that of Comparative Examples 1-7, indicating that the method for preventing and controlling vegetable seedling pith necrosis disease applied by the present invention can effectively inhibit the growth of bacterial pith necrosis pathogens.

[0169] 2. Prevention and control experiments

[0170] Healthy tomato seedlings with consistent growth were taken, and a 5 mm wound was made at the root and stem of the tomato seedlings using a sterile puncher. The diluted solutions of Examples 1-5 and Comparative Examples 1-7 were sprayed on the surface of the tomato seedlings, respectively, and clean water was used as a blank control. After 30 minutes of cultivation, Pseudomonas rugosa Bio-02594 was inoculated at the wounds, and 10 tomato seedlings were treated for each treatment, with three replicates. After 10 days of cultivation, the disease was observed and the incidence was calculated.

[0171] Incidence rate (%) = number of diseased plants / total number of plants × 100%. The measurement results are shown in Table 2.

[0172] Table 2:

[0173]

[0174] From the results in Table 2, it can be seen that the incidence rates of seedlings in Examples 1-5 and Comparative Examples 1-7 are significantly lower than that in the control group, and the incidence rates of the embodiments are lower than those in the comparative examples, indicating that the method for preventing and controlling pith necrosis of vegetable seedlings applied in the present invention can effectively improve the immunity of vegetable seedlings and promote the growth of plants.

[0175] This specific embodiment is merely an explanation of the present application and is not a limitation of the present application. After reading this specification, those skilled in the art may make modifications to the present embodiment without any creative contribution as needed, but such modifications are protected by the patent law as long as they are within the scope of the claims of the present application.

Claims

1. A method for preventing and treating vegetable seedling pith necrosis, characterized in that: The following steps are involved: (1) By weight, 18-22 parts of Coptis chinensis, 3-5 parts of Honeysuckle, 3-5 parts of Forsythia suspensa, 1-3 parts of Portulaca oleracea, 4-6 parts of Taraxacum officinale, and 2-4 parts of Sophora flavescens are mixed to prepare a biological agent; (2) Mix the biological agent and ethionine with water in a mass ratio of 7-9:1:8000-10000 to obtain a diluted agent; (3) Spray the diluted agent onto the surface of vegetable seedlings; The method for preparing the biological agent in step (1) comprises the following steps: S1. Mix and grind coptis root, honeysuckle, forsythia, purslane, dandelion and sophora flavescens by weight and pass through a 60-100 mesh sieve to obtain a mixed powder; S2, adding water to the mixed powder at a mass ratio of 1:8-12 for boiling extraction, cooling to 32-40°C to obtain a water extract, adding composite microorganisms to the water extract for sealed fermentation to obtain a fermentation liquid; S3, adding the mixed salt solution to the fermentation liquid and blending evenly, further sealing and fermenting, centrifuging and drying the fermentation product to obtain a biological agent; In step S2, the composite microorganisms are composed of Bacillus amyloliquefaciens, Bacillus mucilaginosus and Trichoderma with a live cell count ratio of 4-6:3-5:1; The mixed salt solution in step S3 is a mixed solution of magnesium succinate, copper sulfate and deionized water in a mass ratio of 4-6:2-4:

100.

2. The method for preventing and treating vegetable seedling pith necrosis according to claim 1, characterized in that: The biological agent in step (1) comprises the following raw materials in parts by weight: 20 parts of coptis root, 4 parts of honeysuckle, 4 parts of forsythia, 2 parts of purslane, 5 parts of dandelion, and 3 parts of sophora flavescens.

3. The method for preventing and treating vegetable seedling pith necrosis according to claim 1, characterized in that: In the step (2), the mass ratio of the biological agent, ethionine and water is 8:1:9000.

4. The method for preventing and treating vegetable seedling pith necrosis according to claim 1, characterized in that: The viable bacterial count of the composite microorganisms in the water extract in step S2 is 1.8-2.2×10 9 CFU / ml, fermentation time is 24-48h.

5. The method for preventing and treating vegetable seedling pith necrosis according to claim 1, characterized in that: In step S3, the mass ratio of the mixed salt solution to the fermentation liquid is 4-8:

100.

6. The method for preventing and treating vegetable seedling pith necrosis according to claim 1, characterized in that: In step S3, the fermentation temperature is 32-40° C. and the fermentation time is 48-72 hours.

7. The method for preventing and treating vegetable seedling pith necrosis according to claim 1, characterized in that: In step S3, the centrifugal speed is 8000-10000 r / min, the centrifugal time is 10-18 min, the drying temperature is 50-58° C., and the drying time is 6-8 h.

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