Medicinal and edible meal replacement powder with effect of relieving liver injury / inflammation as well as preparation method and application of medicinal and edible meal replacement powder
By developing medicinal and food homologous meal replacement powder containing mulberries, cooked red beans, cooked yams, black sesame, cooked brown rice and walnut kernels, the liver damage and inflammation problems caused by obesity are solved, and the liver damage is repaired and inflammatory response is achieved. Relieve, significantly improving liver function and lipid metabolism induced by a high-fat diet.
Patent Information
- Application Number
- CN202411334250.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-09-24
- Publication Date
- 2024-12-17
AI Technical Summary
The existing technology is difficult to effectively alleviate liver damage and inflammation caused by obesity. Traditional weight loss methods have problems such as many side effects of drug toxicity, poor compliance and high rebound rate. The commercially available meal replacement powder cannot effectively alleviate liver damage and inflammation caused by high-fat diet. .
Develop a medicinal and food homologous meal replacement powder, the formula includes mulberries, cooked red beans, cooked yam, black sesame, cooked brown rice and walnut kernels, which are prepared by hot air drying and high-speed crushing, with scientific and reasonable carbohydrates, proteins and dietary fiber. , helps to repair liver damage and relieve inflammatory responses.
This meal replacement powder can regulate diet, reduce weight gain, improve lipid metabolism disorders, reduce liver damage indicators, regulate the body's inflammatory response, and significantly improve liver damage and inflammation induced by high-fat diets.
Smart Images
Figure CN119138593A_ABST
Abstract
Description
Technical Field
[0001] The present invention relates to the field of functional foods, and in particular to a food and medicine meal replacement powder having the efficacy of alleviating liver damage / inflammation, and a preparation method and application thereof. Background Art
[0002] As modern people's lifestyles and dietary structures change, the number of obese people is increasing rapidly. In the process of lipid metabolism in the human body, fat is usually stored in adipose tissue in the form of triglycerides. Once the entire adipose tissue storage pool is exceeded, circulating lipids begin to accumulate ectopically in non-adipose tissues including the liver, promoting tissue damage through a process called lipotoxicity. In addition, abnormal hypertrophy of adipose tissue will lead to changes in the secretion pattern of adipose cytokines, such as an increase in total cholesterol, total triglycerides, low-density lipoprotein cholesterol, and a decrease in high-density lipoprotein cholesterol, which together with inflammation may lead to liver cell dysfunction.
[0003] At present, the commonly used means of weight loss and lipid reduction are usually drugs and surgery, changes in lifestyle and eating patterns, and long-term and regular exercise. However, due to poor patient compliance, many drug toxicity and side effects, and easy rebound after surgery, the treatment of liver damage / inflammation caused by obesity faces major challenges. Therefore, it is particularly important to find safe, reliable, low-adverse-reaction, and effective alternative treatment methods. As natural products, medicinal and edible substances have many advantages such as a wide variety, low cost, and multiple targets, which are more suitable for the treatment of obesity. At the same time, meal replacement powders with low calories and comprehensive nutrition are a better product form. At present, commercially available meal replacement powders mainly achieve weight loss effects by enhancing satiety and reducing the intake of carbohydrates and fats, while there are few meal replacement powders that can relieve liver damage / inflammation caused by obesity. Therefore, the development of a medicinal and edible meal replacement powder with the effect of relieving liver damage / inflammation has a good market prospect. Summary of the invention
[0004] Technical problem to be solved: In order to solve the problems existing in the prior art, the present invention provides a medicinal and edible meal replacement powder with the effect of alleviating liver damage / inflammation, and a preparation method and application thereof.
[0005] Technical solution: A edible and medicinal meal replacement powder with the effect of relieving liver damage / inflammation, the components and weight composition of the edible and medicinal meal replacement powder formula include: 20-30 parts of mulberries, 20-30 parts of cooked red beans, 15-25 parts of cooked yam, 5-15 parts of black sesame seeds, 5-15 parts of cooked brown rice, and 5-15 parts of walnut kernels.
[0006] Preferably, the composition by weight is: 26 parts of mulberries, 26 parts of cooked red beans, 16 parts of cooked yam, 8 parts of black sesame seeds, 8 parts of cooked brown rice, 8 parts of inulin and 8 parts of walnut kernels.
[0007] A method for preparing a edible and medicinal meal replacement powder having the effect of relieving liver damage / inflammation, comprising the following steps: (1) removing impurities and washing mulberries, cooked red beans, cooked yam, black sesame, cooked brown rice, and walnut kernels, and then drying them with hot air at a drying temperature of 100-120° C. and a drying time of 10-20 min; (2) mixing the dried mulberries, cooked red beans, cooked yam, black sesame, cooked brown rice, walnut kernels and inulin in proportion, and crushing them at high speed at a rotation speed of 3000-4000 rpm, crushing them for 10-20 s each time, and crushing them 4-5 times in total.
[0008] The preferred preparation process is as follows: (1) removing impurities and washing mulberries, cooked red beans, cooked yam, black sesame, cooked brown rice, and walnut kernels, and then drying them with hot air at a drying temperature of 110° C. for 15 min; (2) mixing the dried mulberries, cooked red beans, cooked yam, black sesame, cooked brown rice, walnut kernels and inulin in proportion, and pulverizing them at high speed at a speed of 3500 rpm, pulverizing for 15 s each time, and pulverizing them 4 times in total to obtain the product.
[0009] The use of the above-mentioned medicinal and edible meal replacement powder in the preparation of products for relieving liver damage / inflammation.
[0010] The above-mentioned liver damage / inflammation is liver damage / inflammation caused by a long-term high-fat diet.
[0011] The above-mentioned medicine and food meal replacement powder contains scientifically reasonable carbohydrates, proteins and dietary fiber, which can help to self-repair liver damage caused by high-fat diet and relieve the body's inflammatory response induced by high-fat diet. The following is a detailed introduction to the functions of each component:
[0012] Mulberries are fruits of the mulberry tree, a plant of the Moraceae family. They are rich in nutrients such as organic acids, vitamins, minerals, amino acids, and a large number of active ingredients such as anthocyanins and non-anthocyanin phenolic compounds. Mulberries have antioxidant activity, immune regulation, blood pressure lowering, blood sugar lowering, and blood lipid lowering effects.
[0013] Red beans belong to the genus Vigna in the Leguminosae family and are rich in protein, vitamins, minerals, dietary fiber, polyphenols, polysaccharides, flavonoids and other substances. Long-term consumption is beneficial to lower fasting blood sugar, glycosylated hemoglobin and low-density lipoprotein cholesterol levels, thereby improving fatty liver, diabetes, cardiovascular and cerebrovascular diseases and other diseases. At the same time, it can also enhance satiety and reduce weight.
[0014] Chinese yam is the dried rhizome of Dioscorea zingiberensis, a plant of the Dioscoreaceae family. It is rich in protein, carbohydrates, dietary fiber, vitamin C, trace elements (I, Ca, Fe, P), polysaccharides, polyphenols, saponins and other ingredients. Among them, Chinese yam polysaccharides composed of mannose, arabinose, xylose, glucose, galactose and a small amount of fucose have multiple functions such as improving immunity, anti-oxidation, regulating the gastrointestinal tract, and lowering blood sugar. Polyphenols are important antioxidants in Chinese yam, mainly composed of a large amount of flavonoids, phenolic acids and tannins. The saponin components in Chinese yam can reduce the level of pro-inflammatory factors in the body, improve the body's oxidative stress, and improve insulin resistance.
[0015] Black sesame is the black seed of Sesame, Sesame family. It is rich in protein and fatty acids, the latter mainly unsaturated fatty acids. Its characteristic substance melanin has multiple functions such as anti-oxidation, liver and kidney protection, and regulation of body lipid metabolism. The unique lignin in black sesame, mainly sesamin and sesamolin, has anti-aging, anti-inflammatory, and slowing myocardial cell apoptosis effects.
[0016] Brown rice is the dried seed of the plant of the genus Oryza, belonging to the family Poaceae. It is rich in dietary fiber, vitamins A, B, E, unsaturated fatty acids, minerals and other nutrients. It also contains a variety of functional ingredients such as oryzanol, tocopherol, ferulic acid, etc. Brown rice plays an important role in protecting the heart, regulating the body's inflammatory response, anti-oxidation and improving sleep.
[0017] Inulin, as a dietary fiber, is decomposed and utilized by intestinal microorganisms to produce metabolites such as short-chain fatty acids, which can regulate the host's lipid metabolism and relieve obesity. It also has multiple functions such as inhibiting pathogenic bacteria, relieving inflammation, and regulating immunity.
[0018] Walnut kernel is the fruit of deciduous tree plants of the genus Juglans in the family Juglandaceae. It is rich in polyphenols, flavonoids, polysaccharides, oleic acid, linoleic acid, linolenic acid and other functional ingredients. The ratio of n6 and n3 unsaturated fatty acids is close to the golden ratio of 6:1. It has extremely high nutritional value and has many health benefits such as anti-oxidation, regulating the immune system, lowering blood lipids and blood pressure.
[0019] Among them, mulberry, red bean, yam and black sesame are all "substances that are traditionally both food and Chinese medicinal materials" approved by the Ministry of Health, and inulin is a "new resource food" approved by the Ministry of Health.
[0020] Beneficial effects: (1) The food-medicine meal replacement powder of the present invention has the function of regulating food intake, reducing weight gain, and slowing down obesity. (2) The food-medicine meal replacement powder of the present invention has the function of regulating adipose cell factors, alleviating lipid metabolism disorders, improving liver damage caused by a high-fat diet, and regulating the body's inflammatory response. BRIEF DESCRIPTION OF THE DRAWINGS
[0021] Figure 1 This is a graph showing the changes in body weight and food intake of mice. * indicates significant differences among the groups (P<0.05), the same below.
[0022] Figure 2 This is a diagram showing the determination of liver function levels in mice.
[0023] Figure 3 This is a diagram showing the measurement of lipid cytokine levels in mouse liver.
[0024] Figure 4 This is a diagram for the determination of inflammatory factors in mouse serum. DETAILED DESCRIPTION
[0025] The food-medicine-meal replacement powder of the present invention has the effect of relieving liver damage / inflammation, and animal experiments are used to study the effect of food-medicine-meal replacement powder in relieving liver damage / inflammation induced by a high-fat diet in vivo. The present invention establishes a liver damage / inflammation mouse model by inducing a long-term high-fat diet, and after gavage with commercially available meal replacement powder and food-medicine-meal replacement powder, the weight and food intake of the mice are recorded, and the content or activity of alanine aminotransferase (ALT), aspartate aminotransferase (AST), total cholesterol (TC), triglycerides (TG), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C) and inflammatory factors tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in the mouse liver is measured, so as to evaluate the effect of food-medicine-meal replacement powder in relieving liver damage / inflammation.
[0026] The present invention is further described in detail below in conjunction with specific embodiments and drawings.
[0027] Example 1
[0028] 1. Preparation of Oral Gavage Samples
[0029] Preparation of food-medicine meal replacement powder solution: Prepare food-medicine meal replacement powder by weight by removing impurities, washing, drying and crushing 26 parts of mulberry, 26 parts of cooked red bean, 16 parts of cooked yam, 8 parts of black sesame, 8 parts of cooked brown rice, 8 parts of inulin and 8 parts of walnut kernel. Dissolve 10g of food-medicine meal replacement powder in 100mL of hot water (90-95℃), stir evenly and set aside.
[0030] Preparation of commercially available meal replacement powder solution: Dissolve 10 g of commercially available meal replacement powder in 100 mL of hot water (90-95° C.), stir well and set aside.
[0031] 2. Animal grouping and modeling
[0032] After 1 week of adaptive feeding, C57BL / 6 female mice were randomly divided into 4 groups, 8 mice in each group, namely blank group, model group, positive control group and medicine and food homologous meal replacement powder group. The blank group was fed with ordinary feed, and the model group, positive control group and medicine and food homologous meal replacement powder group were fed with high-fat feed for 4 weeks. After the model was established, a 5-week intervention was carried out. All samples were gavaged at a dose of 1.0 mL / 20 g·d, and drinking water and feed were supplemented regularly every day. After gavage, the animals were observed, the status of the animals was recorded in time, and the bedding was changed twice a week. The specific grouping and treatment are shown in Table 1.
[0033] Table 1 Animal experimental groups and treatments
[0034]
[0035] 3. Characterization of test results
[0036] (1) Changes in mouse body weight and food intake
[0037] After the high-fat diet model was established, the obese mice were intervened for 5 weeks. The mice were weighed and recorded regularly every week. At the same time, the daily feed intake and remaining amount of the mice were recorded to calculate the daily food intake.
[0038] Results: To evaluate the effects of food-drug meal replacement powder on body weight and food intake in mice with liver injury / inflammation induced by a high-fat diet, weekly body weight and daily food intake were recorded regularly during the intervention period. Figure 1 As shown in the figure, at the 4th week, the weight of mice induced by high-fat diet was higher than that of the blank group (NC). After 5 weeks of intervention, the weight of mice in the model group (MC) increased from 19.88g to 24.26g, while the weight of mice after intervention with medicinal and food meal replacement powder decreased from 20.61g to 19.25g, showing a significant slowdown in weight gain. In terms of food intake during the intervention period, the average daily food intake of mice in the model group increased from 1.87g to 2.14g, while the average daily food intake of mice in the medicinal and food meal replacement powder group (MRP) decreased from 1.71g to 1.20g. Therefore, medicinal and food meal replacement powder can effectively reduce food intake, increase satiety, and thus slow down obesity.
[0039] (2) Liver function of mice
[0040] The liver is the main organ for lipid metabolism. Lipid accumulation can seriously damage the liver. The activity of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) is an important indicator of liver function. When liver cells are damaged, the permeability of the cell membrane changes, and intracellular ALT and AST will leak out, causing abnormal increases in the body's ALT and AST. After taking the mouse liver for liver homogenization, the liver homogenate supernatant was obtained by centrifugation, and the content of ALT and AST in the liver of each group of mice was determined using a fully automatic biochemical analyzer.
[0041] Results: In order to evaluate the effect of the food-drug meal replacement powder on the liver function of mice with liver injury / inflammation induced by a high-fat diet, the activities of ALT and AST in the liver of each group of mice were measured. Figure 2 As shown in the figure, compared with the blank group (NC), the ALT and AST levels of the model group (MC) mice were significantly higher than those of the blank group (P<0.05), which were 29.16U / L and 105.25U / L, respectively. After intervention with commercially available meal replacement powder or food-medicine-same meal replacement powder, the ALT and AST levels of obese mice were significantly lower than those of the model group (P<0.05). Among them, the ALT and AST levels in the liver of the food-medicine-same meal replacement powder group (MRP) mice were not significantly different from those of the blank group (P>0.05), which were 21.85U / L and 68.12U / L, respectively. The above results show that food-medicine-same meal replacement powder restored the transaminase level of mice.
[0042] (3) Lipid cytokine levels in mouse liver
[0043] The livers of mice were homogenized and centrifuged to obtain the liver homogenate supernatant. The contents of total cholesterol (TC), triglycerides (TG), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C) in the livers of mice in each group were determined using an automatic biochemical analyzer.
[0044] Results: In order to evaluate the effect of the food-drug meal replacement powder on lipid metabolism in the liver of mice with liver injury / inflammation induced by a high-fat diet, the contents of TC, TG, HDL-C and LDL-C in the liver of each group of mice were measured. Figure 3As shown, compared with the blank group (NC), the TC, TG and HDL-C levels of the model group (MC) mice were significantly higher than those of the blank group (P<0.05), and the LDLD-C content was significantly lower than that of the blank group (P<0.05). After intervention with commercially available meal replacement powder or food-medicine meal replacement powder, the TC, TG, HDL-C and LDL-C levels of obese mice were significantly improved. Among them, the TC, TG, HDL-C and LDL-C levels in the liver of mice in the food-medicine meal replacement powder group (MRP) were not significantly different from those in the blank group (P>0.05). The TC content in the liver of mice in the food-medicine meal replacement powder group was reduced from 3.73mmol / L to 2.23mmol / L, the TG content was reduced from 37.13mmol / L to 15.80mmol / L, the LDL-C content was reduced from 2.37mmol / L to 1.60mmol / L, and the HDL-C content was increased from 1.17mmol / L to 1.90mmol / L. The above results show that food-medicine meal replacement powder restored the lipid metabolism level of mice.
[0045] (4) Inflammation in mouse serum
[0046] Obesity often causes low-grade inflammation in the body. Tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) are important indicators for assessing the body's inflammatory status. Mouse serum was obtained and the ELISA kit was used to determine the levels of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in the serum of each group of mice.
[0047] Results: In order to evaluate the effect of the food-drug meal replacement powder on the inflammatory status in the serum of mice with liver injury / inflammation induced by a high-fat diet, the levels of TNF-α and IL-6 in the serum of each group of mice were measured. Figure 3 As shown in the figure, compared with the blank group (NC), the TNF-α and IL-6 levels of the model group (MC) mice were significantly higher than those of the blank group (P<0.05), and after intervention with commercially available meal replacement powder or food-medicine meal replacement powder, the TNF-α and IL-6 levels of obese mice were significantly lower than those of the model group (P<0.05), among which, the TNF-α and IL-6 levels in the liver of the food-medicine meal replacement powder group (MRP) mice were reduced from 813.75pg / mL to 489.47pg / mL and from 109.35pg / mL to 85.01pg / mL, respectively. The above results indicate that food-medicine meal replacement powder regulates the inflammatory response in mice.
[0048] The above examples are only for illustrating the technical concept and features of the present invention, and their purpose is to enable people familiar with the technology to understand the content of the present invention and implement it accordingly, and they cannot be used to limit the protection scope of the present invention. Any equivalent transformation or modification made according to the spirit of the present invention should be included in the protection scope of the present invention.
Claims
1. A food-drug meal replacement powder with the effect of relieving liver damage / inflammation, characterized in that: The ingredients and weight composition of the medicine and food meal replacement powder formula include: 20-30 parts of mulberries, 20-30 parts of cooked red beans, 15-25 parts of cooked yam, 5-15 parts of black sesame seeds, 5-15 parts of cooked brown rice, and 5-15 parts of walnut kernels.
2. The edible and medicinal meal replacement powder having the effect of relieving liver damage / inflammation according to claim 1, characterized in that: The composition by weight is: 26 parts of mulberries, 26 parts of cooked red beans, 16 parts of cooked yam, 8 parts of black sesame seeds, 8 parts of cooked brown rice, 8 parts of inulin and 8 parts of walnut kernels.
3. The method for preparing a food-medicine meal replacement powder having the effect of alleviating liver damage / inflammation according to claim 1 or 2, characterized in that: The following steps are involved: (1) Mulberries, cooked red beans, cooked yam, black sesame, cooked brown rice, and walnut kernels are removed from impurities and cleaned, and then hot air dried at a drying temperature of 100-120°C for a drying time of 10-20 min; (2) The dried mulberries, cooked red beans, cooked yam, black sesame, cooked brown rice, walnut kernels and inulin are mixed in proportion and crushed at high speed at a speed of 3000-4000 rpm, each crushing lasting 10-20 s, for a total of 4-5 times.
4. The preparation method according to claim 3, characterized in that: (1) Mulberry, cooked red bean, cooked yam, black sesame, cooked brown rice and walnut kernels were removed from impurities and washed, and then hot air dried at a drying temperature of 110°C for 15 min; (2) The dried mulberry, cooked red bean, cooked yam, black sesame, cooked brown rice, walnut kernels and inulin were mixed in proportion, and high-speed crushed at a speed of 3500 rpm, each crushing time of 15 s, and crushed 4 times in total to obtain the product.
5. Use of the food-medicinal meal replacement powder according to claim 1 or 2 in the preparation of a product for relieving liver damage / inflammation.
6. The use according to claim 5, characterized in that: The liver damage / inflammation is liver damage / inflammation caused by a long-term high-fat diet.