Polypeptide composition with repairing and anti-aging effects as well as preparation method and application thereof

Abstract

The invention discloses a polypeptide composition with repairing and anti-aging effects. The polypeptide composition is prepared from the following components in parts by weight: 0.001 to 0.1 part of acetyl tetrapeptide-11, 0.001 to 0.1 part of acetyl tetrapeptide-9, 0.001 to 0.1 part of acetyl tetrapeptide-2, 0.001 to 0.1 part of acetyl hexapeptide-8, 1 to 100 parts of silibinin, 1 to 500 parts of water and 3 to 500 parts of alcohol solvent. The invention further discloses a preparation process of the polypeptide composition and application of the polypeptide composition in cosmetics. The polypeptide composition disclosed by the invention has good repairing and anti-aging effects, and can be used for reducing skin laxity and wrinkles and improving the elasticity and compactness of skin.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and in particular to a polypeptide composition with repairing and anti-aging effects, and a preparation method and application thereof. Background Art

[0002] In addition to objective factors such as age and genetics, skin aging is also affected by sunlight, environment, health and other factors. Excessive exposure to the sun or exposure to the sun not only accelerates skin aging, but also easily causes spots; living in a heavily polluted environment for a long time or excessive exposure to chemicals that are harmful to the skin will damage the skin and accelerate aging. Modern people live under great pressure and irregular lives, which can overload the skin. A large number of cellular molecular toxins caused by internal or external factors will accumulate in various layers of the skin, resulting in reduced skin elasticity, dull skin color, pigmentation and accelerated skin aging.

[0003] With the development of economy, people's demand for skin anti-aging and repair is gradually increasing. Therefore, more and more scientific research institutions and skin care companies are committed to the research of repair and anti-aging products to obtain methods or skin care products that can effectively resist skin aging or repair aged skin. At present, the most commonly used anti-aging ingredients in skin care products include: plant extracts, peptides, bosine, vitamin C, vitamin E, fullerene, retinoids, etc. However, any ingredient has certain limitations. In view of the shortcomings of existing ingredients, many researchers have combined multiple ingredients or modified the molecular structure of existing ingredients to obtain more ideal effects.

[0004] In recent years, the physiological functions of polypeptides in organisms have received more and more attention. A large number of research results show that proteins are not easily digested and absorbed by the human body due to their large molecular weight and complex structure, which affects the effective performance of their physiological functions and nutritional value. Polypeptides are a class of compounds that are simpler in structure and smaller in molecular weight than proteins and are connected by peptide bonds from 2 to 16 amino acids. Polypeptides are biologically active substances involved in various cell functions in organisms. Scientific discoveries have shown that almost all cells are regulated by polypeptides, such as cell differentiation, neurohormone transmitter regulation, and immune regulation, which are closely related to active polypeptides. It has the dual function of regulating the physiological functions of the body and providing nutrition for the body. Since polypeptides have physiological activities such as regulating the autonomic nervous system, activating cellular immune function, improving cardiovascular function, and anti-aging, this provides a theoretical basis for the development of peptide drugs and peptide health products.

[0005] Therefore, it is necessary to develop a polypeptide composition with repairing and anti-aging effects. Summary of the invention

[0006] The technical problem to be solved by the present invention is to provide a polypeptide composition, which has good repair and anti-aging effects, can reduce skin sagging and wrinkles, and improve skin elasticity and firmness.

[0007] In order to solve the above technical problems, the present invention provides the following technical solutions:

[0008] The first aspect of the present invention provides a polypeptide composition with repair and anti-aging effects, wherein the polypeptide composition comprises the following components by weight:

[0009]

[0010] Cells are the basic units of the skin and the basic units of the structure and function of human skin. If you want to revitalize the skin, it is best to repair the two types of cells that have a greater impact on the skin - basal cells and fibroblasts. This is because basal cells play a key role in maintaining the structure and function of the epidermis, while fibroblasts provide support for the elasticity and structure of the skin. Basal cells are the bottom cells of the epidermis and are responsible for constantly dividing and generating new cells. These new cells gradually move upward and eventually form the various layers of the epidermis, including the stratum corneum. Therefore, by promoting the differentiation and regeneration of basal cells, the overall epidermal quality can be improved. Fibroblasts are responsible for synthesizing structural proteins such as collagen and elastin, which are responsible for maintaining the elasticity and structure of the skin. As we age, the production of these structural proteins in the skin decreases, leading to sagging skin and the formation of wrinkles. By promoting the synthesis of these proteins by fibroblasts, the elasticity and firmness of the skin can be improved.

[0011] The inventors have found through a large number of experiments that when acetyl tetrapeptide-11, acetyl tetrapeptide-9, acetyl tetrapeptide-2, acetyl hexapeptide-8 and silybin are used in a certain proportion, a synergistic effect can be produced, which can significantly improve the skin repair effect and inhibit skin aging. This is because the four polypeptides of acetyl tetrapeptide-11, acetyl tetrapeptide-9, acetyl tetrapeptide-2 and acetyl hexapeptide-8 can act on basal cells or fibroblasts through different mechanisms, promote the synthesis of collagen, repair the skin barrier, and delay the aging of the skin, thereby achieving a complementary effect, and ultimately improving the skin repair effect and anti-aging effect.

[0012] Specifically, acetyl tetrapeptide-11 can act on basal cells, promote the production of epidermal polysaccharide-1, enhance epidermal cohesion, and promote barrier repair; at the same time, it acts on the DEJ connecting layer between the dermis and the epidermis, promotes the synthesis of type 17 collagen, and strengthens the close connection between the epidermis and the basement membrane. Acetyl tetrapeptide-9 can act on fibroblasts to promote the synthesis of type I collagen. Acetyl tetrapeptide-2 can act on fibroblasts, targeting dermal fibroblasts, activating the initiating factors for the correct assembly of elastic fibers: fibulin 5 (FBLN5) and lysyl oxidase 1 (LOXL1), and increasing the levels of these two proteins, thereby making elastic fibers healthy. Acetyl hexapeptide-8 can inhibit the excessive inflammatory response of alarmin IL-33, reduce inflammatory aging, and at the same time reduce AGEs (end products of glycation) and ALEs (lipid peroxidation products), improve skin brightness and glossiness, and the skin will appear whiter and more translucent. Silybin, also known as silymarin, is a natural active substance extracted from the dried fruit of the Asteraceae plant, Milk thistle. Studies have found that silybin has good exogenous and endogenous antioxidant effects and can help prevent photoaging.

[0013] In the composition provided by the present invention, the content of acetyl tetrapeptide-11 is 0.001 to 0.1 parts by mass, for example, it can be 0.001 parts, 0.005 parts, 0.01 parts, 0.02 parts, 0.04 parts, 0.05 parts, 0.06 parts, 0.08 parts, 0.1 parts, etc., but it is not limited to the listed values, and other unlisted values ​​or ranges within the numerical range are also applicable.

[0014] In the composition provided by the present invention, the content of acetyl tetrapeptide-9 is 0.001 to 0.1 parts by mass, for example, it can be 0.001 parts, 0.005 parts, 0.01 parts, 0.02 parts, 0.04 parts, 0.05 parts, 0.06 parts, 0.08 parts, 0.1 parts, etc., but it is not limited to the listed values, and other unlisted values ​​or ranges within the numerical range are also applicable.

[0015] In the composition provided by the present invention, the content of acetyl tetrapeptide-2 is 0.001 to 0.1 parts by mass, for example, it can be 0.001 parts, 0.005 parts, 0.01 parts, 0.02 parts, 0.04 parts, 0.05 parts, 0.06 parts, 0.08 parts, 0.1 parts, etc., but it is not limited to the listed values, and other unlisted values ​​or ranges within the numerical range are also applicable.

[0016] In the composition provided by the present invention, the content of acetyl hexapeptide-8 is 0.001 to 0.1 parts by mass, for example, it can be 0.001 parts, 0.005 parts, 0.01 parts, 0.02 parts, 0.04 parts, 0.05 parts, 0.06 parts, 0.08 parts, 0.1 parts, etc., but it is not limited to the listed values, and other unlisted values ​​or ranges within the numerical range are also applicable.

[0017] In the composition provided by the present invention, the content of silybin is 1 to 100 parts by mass, for example, 1 part, 5 parts, 10 parts, 20 parts, 30 parts, 50 parts, 60 parts, 80 parts, 100 parts, etc., but is not limited to the listed values, and other unlisted values ​​or ranges within the numerical range are also applicable.

[0018] In the composition provided by the present invention, the solubility of the polypeptide and silybin is different, the polypeptide is a water-soluble component, and silybin is an alcohol-soluble component. In order to ensure the solubility of the composition, a certain amount of alcohol solvent is added to the composition to dissolve silybin. The alcohol solvent includes but is not limited to one or more of ethoxydiglycol, butylene glycol, glycerol polyether-26, 1,3-propylene glycol, glycerol, and dipropylene glycol. Preferably, the mass ratio between silybin and the alcohol solvent is 1:3 to 5, for example, it can be 1:3, 1:4, 1:5, etc.

[0019] In some preferred embodiments of the present invention, the polypeptide composition comprises the following components by weight:

[0020]

[0021] In some preferred embodiments of the present invention, the polypeptide composition comprises the following components by weight:

[0022]

[0023] In some preferred embodiments of the present invention, the polypeptide composition further comprises nano-osmotic enhancer.

[0024] In the present invention, the nano-penetrating agent comprises the following components by mass fraction: 5% hydroxyethyl chitosan, 3% 1,2-pentanediol, 0.3% p-hydroxyacetophenone, and 91.7% water. In this nano-penetrating agent, the main component is hydroxyethyl chitosan, which forms nanoparticles by self-assembly in an aqueous solution, and can encapsulate polypeptide components therein, thereby increasing the stability of the active ingredients; in addition, the nanoparticles formed by the nano-penetrating agent have the characteristics of small particle size and easy skin penetration, which can significantly improve the penetration effect, thereby promoting the polypeptide to quickly and accurately reach the basal layer of human skin, and to retain for a long time, slow-release and control-release active substances, significantly increase the skin retention of active substances, and improve their transdermal penetration bioavailability.

[0025] In the composition provided by the present invention, the content of the nano-osmotic enhancer is 50 to 400 parts by mass, for example, it can be 50 parts, 100 parts, 150 parts, 200 parts, 250 parts, 300 parts, 350 parts, 400 parts, etc., but is not limited to the listed values, and other unlisted values ​​or ranges within the numerical range are also applicable.

[0026] In some preferred embodiments of the present invention, the content of nano-osmotic enhancer in the polypeptide composition is 100 to 200 parts.

[0027] In some preferred embodiments of the present invention, the polypeptide composition further comprises glycerol glucoside.

[0028] Glyceryl glucoside is a type of glycoside compound formed by glycerol molecules and glucose molecules connected by glycosidic bonds. It has excellent transdermal properties and can directly reach the dermis of the skin, thus playing a good role in deep hydration and long-lasting moisturizing. Secondly, glyceryl glucoside can also promote the production of the antioxidant skin protection enzyme "superoxide dismutase 1" (SOD1), strengthen the skin's own immune system, and activate the cell's antioxidant capacity. In addition, glyceryl glucoside can also effectively promote the expression of collagen 1 and elastin genes, promote collagen and elastin synthesis, increase the metabolic rate of aging cells, strengthen the skin base, and smooth fine lines.

[0029] In the composition provided by the present invention, the content of glycerol glucoside is 10 to 300 parts by mass, for example, it can be 10 parts, 50 parts, 100 parts, 150 parts, 200 parts, 250 parts, 300 parts, etc., but is not limited to the listed values, and other unlisted values ​​or ranges within the numerical range are also applicable.

[0030] In some preferred embodiments of the present invention, the content of glycerol glucoside in the polypeptide composition is 50 to 200 parts.

[0031] The second aspect of the present invention provides a method for preparing the polypeptide composition having repair and anti-aging effects, comprising the following steps:

[0032] Add the formulated amount of acetyl tetrapeptide-11, acetyl tetrapeptide-9, acetyl tetrapeptide-2, and acetyl hexapeptide-8 into water and stir until completely dissolved to obtain phase A;

[0033] Add the formulated amount of silybin to the alcohol solvent and stir until completely dissolved to obtain phase B;

[0034] The phase A and the phase B are mixed and stirred evenly to obtain the polypeptide composition.

[0035] When the polypeptide composition includes nano-osmotic enhancer, the polypeptide can be firstly coated with the nano-osmotic enhancer and then mixed with the alcohol solution of silybin to prepare a uniform polypeptide composition.

[0036] The third aspect of the present invention provides the use of the polypeptide composition with repair and anti-aging effects in cosmetics. The cosmetics use the polypeptide composition as an active ingredient and include a cosmetically acceptable matrix.

[0037] In some embodiments, the amount of the polypeptide composition added is 0.01% to 10% by mass percentage of the cosmetic, for example, it can be any value among 0.01%, 0.1%, 0.5%, 1%, 2%, 3%, 4%, 5%, 6%, 8%, 10%, or a range consisting of any two values ​​thereof. The amount of the matrix acceptable to the cosmetic added is 90% to 99.99%, for example, it can be any value among 90%, 92%, 95%, 98%, 99%, 99.99%, or a range consisting of any two values ​​thereof. However, since the above ratios vary according to the formula of the cosmetic of the present invention and its specific application site (face or hand) or the required applicable amount, the above ratios cannot be understood as limiting the scope of the present invention in any aspect.

[0038] The cosmetics of the present invention can be prepared in various forms. For example, the cosmetics of the present invention can be prepared in any dosage form conventionally prepared in this field, including but not limited to solutions, suspensions, ointments, gels, creams, emulsions, loose powders, facial cleansers, essential oils, foundations, emulsion foundations, wax foundations, sprays, etc. In addition, specifically, it can be selected from various types in the group of lotions, skin lotions, softeners, toners, astringents, skin care lotions, emulsions, moisturizing lotions, nourishing lotions, massage creams, nourishing creams, moisturizing creams, hand creams, essences, facial masks, soaps, shampoos, cleansing foams, cleansing milks, cleansing creams, body lotions, shower gels, lotions, lipsticks, primers, foundation liquids, pressed powders, and loose powders, but is not limited thereto.

[0039] In addition to its active ingredients, the cosmetics of the present invention may contain a matrix acceptable in cosmetics. The matrix includes, but is not limited to, one or more of solvents, cosolvents, oils, moisturizers, thickeners, pH regulators, emulsifiers, preservatives, essences, etc. The ingredients that can be used as the matrix are known in the art, and those skilled in the art can select and use appropriate materials / compositions.

[0040] In some preferred embodiments of the present invention, in the cosmetic, the mass concentration of each component in the polypeptide composition is:

[0041]

[0042]

[0043] Compared with the prior art, the present invention has the following beneficial effects:

[0044] The polypeptide composition provided by the present invention has good repair and anti-aging effects, can reduce skin sagging and wrinkles, and improve skin elasticity and firmness. DETAILED DESCRIPTION

[0045] The present invention is further described below in conjunction with specific embodiments so that those skilled in the art can better understand the present invention and implement it, but the embodiments are not intended to limit the present invention.

[0046] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art of the present invention. The terms used in the specification of the present invention are only for the purpose of describing specific embodiments and are not intended to limit the present invention. The term "and / or" used herein includes any and all combinations of one or more related listed items.

[0047] Unless otherwise specified, the experimental methods used in the following examples and comparative examples are all conventional methods, and the materials, reagents, etc. used are all commercially available unless otherwise specified.

[0048] In the following embodiments and comparative examples:

[0049] The formula of nano-osmotic enhancer is: 5% hydroxyethyl deacetylated chitosan, 3% 1,2-pentanediol, 0.3% p-hydroxyacetophenone, and 91.7% water.

[0050] Example 1

[0051] This embodiment provides a polypeptide composition with repair and anti-aging effects, including the following components by weight:

[0052]

[0053]

[0054] It should be noted that a certain amount of thickener is added to the polypeptide composition of this embodiment, the purpose of which is to transform the polypeptide composition from a solution to a gel, thereby preventing the precipitation of silybin in the subsequent test process and affecting the accuracy of the test results.

[0055] The preparation method of the polypeptide composition of this embodiment is as follows:

[0056] Add the formulated amount of acetyl tetrapeptide-11, acetyl tetrapeptide-9, acetyl tetrapeptide-2, and acetyl hexapeptide-8 into water and stir until completely dissolved to obtain phase A;

[0057] Add the formulated amount of silybin to ethoxydiglycol and stir until completely dissolved to obtain phase B;

[0058] Mix phase A and phase B, add ammonium acryloyldimethyltaurate, and stir evenly to obtain a gel-like polypeptide composition sample.

[0059] Embodiments 2 to 6

[0060] The preparation methods of the polypeptide compositions of Examples 2 to 6 are the same as those of Example 1, and their formulations are shown in Table 1.

[0061] Table 1

[0062]

[0063] Comparative Examples 1 to 7

[0064] The preparation methods of the polypeptide compositions of Comparative Examples 1 to 7 are the same as those of Example 1, and their formulations are shown in Table 2.

[0065] Table 2

[0066]

[0067] 1. Collagen and elastin expression test

[0068] The polypeptide composition samples of each embodiment and comparative example were diluted with water to a total concentration of 1wt%, and then 36 6-day-old zebrafish were divided into 3 groups and exposed to the sample solution. A blank control group was set up at the same time. After 24 hours of exposure, RNA was extracted from the zebrafish, cDNA was synthesized, and real-time PCR amplification was performed. β-actin was used as a housekeeping gene, Ct was used as the amplification result, and the relative expression of Elna was calculated and statistically analyzed. The results are shown in Table 3.

[0069] Table 3

[0070]

[0071]

[0072]

[0073] From the results in Table 3, it can be seen that compared with the blank control group, in Examples 1 to 6, when acetyl tetrapeptide-11, acetyl tetrapeptide-9, acetyl tetrapeptide-2, acetyl hexapeptide-8 and silybin are compounded in a certain ratio, the obtained samples can significantly promote the expression of col1a1a, col1a1b, col1a2 and elastin gene Elastin, that is, the samples have the effect of promoting the regeneration of type I collagen and elastin, which shows that the polypeptide composition of the embodiment of the present invention has anti-wrinkle and firming effects.

[0074] In Comparative Example 7, when the sample contained only 0.1 parts of acetyl tetrapeptide-11 and 0.1 parts of acetyl tetrapeptide-9, the promotion rate of the composition for type I collagen genes col1a1a, col1a2 and elastin gene Elastin was significantly reduced. In Comparative Example 1, when the sample did not contain silybin, the promotion effect on the expression of type I collagen gene and elastin gene Elastin was also significantly reduced. Similarly, in Comparative Examples 1 to 5, when the sample did not contain any of acetyl tetrapeptide-11, acetyl tetrapeptide-9, acetyl tetrapeptide-2, and acetyl hexapeptide-8, the promotion effect on the expression of type I collagen gene and elastin gene Elastin was also significantly reduced. This shows that in the polypeptide composition of the present invention, when acetyl tetrapeptide-11, acetyl tetrapeptide-9, acetyl tetrapeptide-2, acetyl hexapeptide-8 and silybin are used in combination, a synergistic effect can be produced, which promotes the regeneration of type I collagen and elastin. When any of the components is missing, the promoting effect of the composition on type I collagen and elastin will be significantly reduced.

[0075] In Comparative Example 6, when the content of silybin in the sample is only 0.5 parts, which is not within the range of 1 to 100 parts specified in the present invention, the promoting effect of the sample on the expression of type I collagen gene and elastin gene Elastin is also reduced to a certain extent, which indicates that acetyl tetrapeptide-11, acetyl tetrapeptide-9, acetyl tetrapeptide-2, acetyl hexapeptide-8 and silybin can only produce a synergistic effect on the regeneration of type I collagen and elastin when they are compounded in a certain proportion.

[0076] 2. Cell Proliferation Assay of Fibroblasts

[0077] Fibroblasts were inoculated in a 96-well plate. When the cell plating rate in the 96-well plate reached 20% to 30%, the drugs were administered in groups, with 3 replicates in each group. 200 μL of culture medium was added to each well of the blank control group (BC), and 200 μL of culture medium containing 2.5 wt% of the sample to be tested was added to each well of the sample group. After the administration was completed, the 96-well plate was placed in a CO2 incubator (37°C, 5% CO2) and cultured for 24h, 48h, and 72h, respectively. For groups that need to be incubated for 48h and 72h, half of the liquid was replaced every day. After the cells were incubated for 24h, 48h, and 72h, the supernatant was discarded, and MTT working solution (0.5mg / mL, prepared and used immediately) was added, and incubated at 37°C in the dark for 4h. After the incubation was completed, the supernatant was discarded, 150 μL DMSO was added to each well, and the OD value was read at 490nm. The test results are shown in Table 4.

[0078] Calculation of relative cell viability: relative cell viability (%) = (OD of sample well - OD of zero-adjusting well) / (OD of solvent control well - OD of zero-adjusting well) * 100%.

[0079] Table 4

[0080]

[0081] Note: When the t-test method was used for statistical analysis, the significance was indicated by *, P-value < 0.05 was indicated by *, and P-value < 0.01 was indicated by ** compared with the BC group.

[0082] As can be seen from the results of Table 4, compared with the blank control group, the embodiments and comparative examples have a certain improvement in the activity of fibroblasts after 24h, 48h and 72h of administration. Among them, in comparative examples 1 to 6, after 24h of administration, the cell activity is significantly improved, and the proliferation of fibroblasts is significantly improved. However, after 48h and 72h of administration, there is no significant change in cell activity, and there is no significant improvement in the proliferation of fibroblasts. In Example 1, after 24h, 48h and 72h of administration, the cell activity is significantly improved, and the proliferation of fibroblasts is significantly improved. This shows that when acetyl tetrapeptide-11, acetyl tetrapeptide-9, acetyl tetrapeptide-2, acetyl hexapeptide-8 and silybin are compounded in a certain ratio, they can produce a synergistic effect on the proliferation of fibroblasts.

[0083] 3. Cell Proliferation Test of Keratinocytes

[0084] Cell activity refers to the proportion of healthy cells in a sample population. Enhancing the cell activity of cells can stabilize the skin barrier and promote skin renewal. Cell migration refers to the movement of cells after receiving migration signals or being stimulated by certain substances. Increased cell migration ability indicates that it has the effect of promoting wound repair. Therefore, this test is based on keratinocytes, and by detecting changes in cell proliferation and cell migration ability, the repair effect of the sample to be tested is evaluated.

[0085] The cells used in this test are keratinocytes, batch number: Ep23103101, provided by Guangdong Boxi Biotechnology Co., Ltd. Keratinocytes were inoculated in a 96-well plate. When the plating rate of cells in the 96-well plate reached 20% to 30%, the drugs were administered in groups, with 3 replicates in each group. 200 μL of culture medium was added to each well of the blank control group, and 200 μL of culture medium containing 5% of the sample to be tested was added to each well of the sample group. After the administration was completed, the 96-well plate was placed in a CO2 incubator (37°C, 5% CO2) and cultured for 24h, 48h, and 72h, respectively. For groups that need to be incubated for 48h and 72h, half of the fluid was changed every day. After the cells were incubated for 24h, 48h, and 72h, the supernatant was discarded, and MTT working solution (0.5mg / mL, prepared and used immediately) was added, and the cells were incubated at 37°C in the dark for 4h. After the incubation, the supernatant was discarded, 150μLDMSO was added to each well, and the OD value was read at 490nm. The test results are shown in Table 5.

[0086] Table 5

[0087]

[0088] As can be seen from the results of Table 5, compared with the blank control group, the embodiments and comparative examples have a certain improvement in cell viability after 24h, 48h and 72h of administration. Among them, in comparative examples 1 to 6, after 48h and 72 of administration, the cell viability is significantly improved, and the proliferation of keratinocytes is significantly improved. However, after 24h of administration, there is no significant change in cell viability, and there is no significant improvement in the proliferation of keratinocytes. In Example 1, after 24h, 48h and 72h of administration, the cell viability is significantly improved, and the proliferation of keratinocytes is significantly improved. This shows that when acetyl tetrapeptide-11, acetyl tetrapeptide-9, acetyl tetrapeptide-2, acetyl hexapeptide-8 and silybin are compounded in a certain ratio, they can produce a synergistic effect on the proliferation of keratinocytes.

[0089] 4. Keratinocyte Migration Test

[0090] Keratinocytes were inoculated in a 6-well plate. When the cell plating rate in the 6-well plate reached 70% to 80%, the drugs were administered in groups, with 3 replicates in each group. 2 mL of culture medium was added to each well of the blank control group, and 2 mL of culture medium containing 5% of the sample to be tested was added to each well of the sample group. After the administration, the 6-well plate was placed in a CO2 incubator (37°C, 5% CO2) and cultured for 24 hours. After the incubation, scratches were performed. After the scratch, the cells were washed 3 times with PBS, cell culture medium was added, and cultured in a CO2 incubator (37°C, 5% CO2) for 24 hours. Take a picture under a 4x microscope at 0 hours after the scratch, wash once with PBS after 24 hours, and take a picture under a 4x microscope. The test results are shown in Table 6.

[0091] Calculation of the improvement rate: Improvement rate (%) = (sample group - blank control group) / blank control group × 100%.

[0092] Table 6

[0093] Group The average relative mobility Relative mobility SD P-value Improvement rate (vsBC) BC 1.00 0.02 / / Example 1 1.20 0.02 0.001** 20％ Comparative Example 1 1.11 0.05 0.003** 11％ Comparative Example 2 1.08 0.04 0.006** 8％ Comparative Example 3 1.08 0.05 0.002** 8％ Comparative Example 4 1.10 0.04 0.004** 10％ Comparative Example 5 1.07 0.04 0.005** 7％ Comparative Example 6 1.12 0.02 0.004** 12％

[0094] Please refer to Table 6. Compared with the blank control group, the samples of the embodiment and the comparative example have a significant effect on improving the relative mobility of cells, which is helpful for skin repair. Among them, the sample of the embodiment has the best effect on promoting the relative mobility of cells, and its improvement rate reaches 20%.

[0095] In summary, the polypeptide composition provided by the present invention has good repair and anti-aging effects, can reduce skin sagging and wrinkles, and improve skin elasticity and firmness.

[0096] The above-described embodiments are only preferred embodiments for fully illustrating the present invention, and the protection scope of the present invention is not limited thereto. Equivalent substitutions or changes made by those skilled in the art based on the present invention are within the protection scope of the present invention. The protection scope of the present invention shall be subject to the claims.

Claims

1. A polypeptide composition with repair and anti-aging effects, characterized in that: The polypeptide composition comprises the following components by weight:

2. A polypeptide composition with repair and anti-aging effects according to claim 1, characterized in that: The polypeptide composition comprises the following components by weight:

3. A polypeptide composition with repair and anti-aging effects according to claim 1, characterized in that: The alcohol solvent includes one or more of ethoxydiglycol, butylene glycol, glycerol polyether-26, 1,3-propylene glycol, glycerol, and dipropylene glycol.

4. A polypeptide composition with repair and anti-aging effects according to claim 1, characterized in that: The polypeptide composition also contains 50 to 400 portions of nano-osmotic enhancer; The nano-osmotic enhancer comprises the following components by mass fraction: 5% hydroxyethyl deacetylated chitosan, 3% 1,2-pentanediol, 0.3% p-hydroxyacetophenone, and 91.7% water.

5. The polypeptide composition with repair and anti-aging effects according to claim 1, characterized in that: The polypeptide composition also contains 100 to 200 portions of nano-osmotic enhancer.

6. A polypeptide composition with repair and anti-aging effects according to claim 1, characterized in that: The polypeptide composition also contains 10 to 300 parts of glycerol glucoside.

7. A polypeptide composition with repair and anti-aging effects according to claim 6, characterized in that: The polypeptide composition also contains 50 to 200 parts of glycerol glucoside.

8. The method for preparing the polypeptide composition with repair and anti-aging effects according to any one of claims 1 to 7, characterized in that: The following steps are involved: Add the formulated amount of acetyl tetrapeptide-11, acetyl tetrapeptide-9, acetyl tetrapeptide-2, and acetyl hexapeptide-8 into water and stir until completely dissolved to obtain phase A; Add the formulated amount of silybin to the alcohol solvent and stir until completely dissolved to obtain phase B; The phase A and the phase B are mixed and stirred evenly to obtain the polypeptide composition.

9. Use of the polypeptide composition with repairing and anti-aging effects as claimed in any one of claims 1 to 7 in cosmetics.

10. The use according to claim 9, characterized in that: In the cosmetic, the mass concentration of each component in the polypeptide composition is: Acetyl tetrapeptide-11 0.00001%~0.001% Acetyl tetrapeptide-9 0.00001%~0.001% Acetyl tetrapeptide-2 0.00001%~0.001% Acetyl hexapeptide-8 0.00001%~0.001% Silybin 0.01~1%.

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