Traditional Chinese medicine preparation containing semen cuscutae fermentation extract as well as preparation and application of traditional Chinese medicine preparation

Abstract

The invention relates to a traditional Chinese medicine preparation containing semen cuscutae extract and a preparation method and application thereof.The traditional Chinese medicine preparation is a granule, the traditional Chinese medicine compound granule is prepared from semen cuscutae, herba taxilli, radix dipsaci, radix angelicae sinensis, radix scutellariae, radix paeoniae alba, rhizoma chuanxiong, rhizoma atractylodis macrocephalae and colla corii asini, and the preparation method comprises the steps that 1, semen cuscutae is taken to be subjected to enzymolysis, and the extract is obtained; (2) taking the remaining traditional Chinese medicinal materials, and respectively performing water extraction and alcohol extraction to obtain extracts; and (3) preparing a conventional pharmaceutical preparation. The preparation process is simple and easy to operate, the semen cuscutae extract is effectively separated out through an enzymolysis method, the medicine effect is remarkably improved, and the method has high application and popularization value.

Description

Technical Field

[0001] The invention belongs to the technical field of traditional Chinese medicine preparations, and particularly relates to a traditional Chinese medicine compound granule and a preparation method and use thereof. Background Art

[0002] Recurrent spontaneous abortion (RSA) refers to spontaneous abortions that occur three or more times in a row with the same spouse before 28 weeks of pregnancy. Its etiology is complex. At present, studies have shown that genetic, endocrine, immune, prothrombotic, infection, mental, environmental, and male factors are the most common causes. However, 60% to 70% of recurrent spontaneous abortions (URSA) are still of unknown cause, which seriously limits the clinical treatment effect, not only brings a heavy economic burden to patients and their families, but also seriously affects the physical and mental health of patients. Therefore, finding an effective treatment strategy to actively prevent and treat this disease is one of the clinical problems that needs to be solved urgently.

[0003] Decidualization of endometrial stromal cells is an important sign of successful embryo implantation, and the rapid senescence of stromal cells may affect the process of decidualization, thereby affecting embryo implantation and development. In recent years, studies have shown that the senescence of endometrial stromal cells may mediate recurrent spontaneous abortion by affecting decidualization. Therefore, targeting the senescence of endometrial stromal cells may become a potential strategy for the treatment of URSA.

[0004] At present, research shows that Chinese medicine for tonifying the kidney and activating blood circulation is an effective treatment for fighting cell aging. Therefore, Chinese medicine compound based on tonifying the kidney and activating blood circulation has great application potential in improving the aging of URSA endometrial stromal cells. Summary of the invention

[0005] In view of the shortcomings of the prior art, the present invention provides a traditional Chinese medicine preparation containing a fermented dodder extract with a scientific and prudent attitude. Through precise pharmacological experiments, it is confirmed that the fermented dodder extract preparation has excellent therapeutic effects on recurrent spontaneous abortion, especially unexplained recurrent spontaneous abortion, with few side effects and great commercial value.

[0006] One of the purposes of the present invention is to provide a traditional Chinese medicine preparation containing a fermented extract of Cuscuta. The fermented extract of Cuscuta is obtained by fermenting Cuscuta with Aspergillus oryzae and mixed lactic acid bacteria, wherein the mixed lactic acid bacteria is a mixed bacteria of Lactobacillus rhamnosus and Bifidobacterium;

[0007] The preparation method of the Chinese medicine preparation is:

[0008] 1) Cuscuta fermentation extraction: crush the Cuscuta, add it into MRS liquid culture medium, mix it evenly, sterilize it, inoculate Aspergillus oryzae and mixed lactic acid bacteria into MRS liquid culture medium in sequence, and ferment it; wherein, the inoculation interval of Aspergillus oryzae and mixed lactic acid bacteria is 12-36h, the fermentation time is 4-6d, and the fermentation temperature is: 35-38°C; after the fermentation is completed, centrifuge, filter, and take the supernatant for use;

[0009] 2) Take parasitic herb, Dipsacus asper, white peony root, Chuanxiong and donkey-hide gelatin, soak them in warm water, filter and set aside the filtrate; decoct the residue with water, filter and combine the above filtrates to concentrate to a density of 1.23-1.28 g / mL and set aside;

[0010] 3) Take Chinese Angelica, Scutellaria, and Atractylodes macrocephala, add ethanol to extract by reflux, filter, add ethanol to the filtrate to make the alcohol content reach 70%, let stand overnight, filter, and concentrate under reduced pressure to a relative density of 1.22-1.25 g / mL;

[0011] 4) After the extracts of steps 1), 2) and 3) are combined and mixed, common pharmaceutical excipients are added to prepare a conventional preparation.

[0012] In terms of weight ratio, the raw materials in the above-mentioned Chinese medicine preparation are composed of:

[0013] Cuscuta chinensis 60-100 parts by weight Loranthus parasiticus 50-80 parts by weight Dipsacus asper 50-80 parts by weight

[0014] 50-80 weight parts of white peony root 50-80 weight parts of angelica root 50-80 weight parts of scutellaria root 50-80 weight parts

[0015] 50-80 weight parts of Chuanxiong, 40-60 weight parts of Atractylodes macrocephala, 20-40g of donkey-hide gelatin.

[0016] In a preferred embodiment, the inventors found that when the raw materials in the traditional Chinese medicine preparation are composed of the following weight ratios, the preparation obtained has better efficacy:

[0017] 80 weight parts of Cuscuta australis 60 weight parts of Loranthus parasiticus 50 weight parts of Dipsacus asper

[0018] 60 parts by weight of white peony root 60 parts by weight of angelica root 60 parts by weight of scutellaria root

[0019] 60 weight parts of Chuanxiong, 50 weight parts of Atractylodes macrocephala, 30g of donkey-hide gelatin.

[0020] Preferably, the weight ratio of the mixed bacteria of Lactobacillus rhamnosus and Bifidobacterium is 1:1-3. In a preferred embodiment, when the weight ratio of the mixed bacteria of Lactobacillus rhamnosus and Bifidobacterium is 1:2.5, the prepared Chinese medicine preparation has better efficacy.

[0021] Preferably, in the step (1), Aspergillus oryzae is inoculated at an inoculum volume ratio of 3%-7%.

[0022] Preferably, in step (1), the mixed lactic acid bacteria are inoculated at an inoculation rate of 2%-5% by volume.

[0023] Preferably, the extraction step in step (2) is repeated three times, with the first decoction being 2 hours, the second decoction being 1 hour, and the third decoction being 0.5 hours.

[0024] The conventional Chinese medicine preparation of the present invention is an oral preparation. Further, the oral Chinese medicine preparation is a decoction, a pill, a tablet, a mixture, a capsule, a granule, a powder or an ointment.

[0025] The second purpose of the present invention is to provide a medical use of a traditional Chinese medicine preparation containing a fermented extract of Cuscuta australis. Pharmacology and clinical cases show that the traditional Chinese medicine composition of the present invention is effective in treating recurrent spontaneous abortion or threatened abortion in early and mid-pregnancy, with rapid onset of treatment, high total effective rate, low cost, few adverse reactions and low recurrence rate.

[0026] The Chinese medicinal materials in the Chinese medicine preparation of the present invention synergize to inhibit the aging of endometrial stromal cells and improve uterine decidualization. The effect is better than that of the positive control drug aspirin in optimizing the decidual microenvironment and reducing the embryo absorption rate.

[0027] Furthermore, the recurrent spontaneous abortion is spontaneous abortion that occurs three or more times in a row.

[0028] Preferably, the recurrent spontaneous abortion is unexplained recurrent spontaneous abortion.

[0029] In addition, the Chinese medicine composition of the present invention can also be used in combination with other conventional progestogens or drugs for preventing and treating abortion, and the daily dosage can be adjusted according to the needs of the disease.

[0030] Unless otherwise specified, the raw materials of the Chinese medicine composition of the present invention are all purchased conventionally. Any range described herein, including the end value, any value between the end values, and the sub-range formed by the end values, can be used to prevent and treat recurrent miscarriage or threatened miscarriage in early and mid-pregnancy.

[0031] Compared with the prior art, the present invention has the following beneficial effects:

[0032] (1) The biological fermentation method greatly improves the extraction rate of the effective ingredients of Cuscuta seeds, and the efficacy is more significant;

[0033] (2) The present invention adopts Chinese medicine preparations, and the raw materials and auxiliary materials used are less toxic, suitable for use before and after pregnancy, and greatly protect the health of the fetus and the mother;

[0034] (3) The preparation method of the present invention is simple and easy to operate, has low cost, and is suitable for industrial promotion and application. BRIEF DESCRIPTION OF THE DRAWINGS

[0035] Figure 1 The effect of the Chinese medicine composition of the present invention on the serum IGFBP-1 content in mice with unexplained recurrent spontaneous abortion;

[0036] Figure 2 Some senescent cells show β-galactosidase staining after staining.

[0037] Figure 3 The invention discloses the effect of the Chinese medicinal composition on the senescence of endometrial stromal cells in mice with recurrent spontaneous abortion.

[0038] Figure 4 The invention discloses an effect of the Chinese medicinal composition on the embryo absorption rate of mice with unexplained recurrent spontaneous abortion. DETAILED DESCRIPTION

[0039] In order to explain the present invention more clearly, the present invention is further described below in conjunction with preferred embodiments. It should be understood by those skilled in the art that the following specific description is illustrative rather than restrictive, and should not be used to limit the scope of protection of the present invention.

[0040] Example 1 Preparation of granules:

[0041] Formula: Cuscuta 80g, Morus alba 60g, Dipsacus asper 50g

[0042] White Peony 60g Angelica 60g Scutellaria 60g

[0043] Chuanxiong 60g Atractylodes 50g Donkey-hide gelatin 30g

[0044] Preparation method:

[0045] 1) Cuscuta fermentation extraction: Cuscuta is crushed, added to MRS liquid culture medium, mixed, sterilized, and Aspergillus oryzae and mixed lactic acid bacteria are inoculated in MRS liquid culture medium in sequence for fermentation; wherein, the inoculation interval of Aspergillus oryzae and mixed lactic acid bacteria is 16h, the fermentation time is 4d, and the fermentation temperature is: 35°C; after the fermentation is completed, centrifugation, filtration, and the supernatant is taken for standby use; the amount of the mixed bacteria of Lactobacillus rhamnosus and Bifidobacterium is 1:2.5; Aspergillus oryzae is inoculated at an inoculation rate of 5% by volume; and the mixed lactic acid bacteria is inoculated at an inoculation rate of 3% by volume;

[0046] 2) Take mistletoe, radix polygoni multiflori, white peony root, rhizoma chuanxiong, and donkey-hide gelatin, soak them in warm water, filter them, and set aside the filtrate; repeat the extraction step three times, decocting for 2 hours for the first time, 1 hour for the second time, and 0.5 hour for the third time; decoct the residues with water, filter them, combine the above filtrates and concentrate them to a density of 1.28 g / mL, and set aside;

[0047] 3) Take Chinese Angelica, Scutellaria, and Atractylodes macrocephala, add ethanol to extract by reflux, filter, add ethanol to the filtrate to make the alcohol content reach 70%, let stand overnight, filter, and concentrate under reduced pressure to a relative density of 1.22 g / mL;

[0048] 4) After the extracts in steps 1), 2) and 3) are combined and mixed, common pharmaceutical excipients are added to prepare granules.

[0049] Example 2 Preparation of granules:

[0050] Formula: Cuscuta 80g, Morus alba 60g, Dipsacus asper 50g

[0051] White Peony 60g Angelica 60g Scutellaria 60g

[0052] Chuanxiong 60g Atractylodes 50g Donkey-hide gelatin 30g

[0053] Preparation method:

[0054] 1) Cuscuta fermentation extraction: Cuscuta is crushed, added to MRS liquid culture medium, mixed, sterilized, Aspergillus oryzae and mixed lactic acid bacteria are inoculated in MRS liquid culture medium in sequence for fermentation; wherein, the inoculation interval of Aspergillus oryzae and mixed lactic acid bacteria is 12h, the fermentation time is 6d, and the fermentation temperature is 38°C; after the fermentation is completed, centrifugation, filtration, and the supernatant is taken for standby use; the amount of the mixed bacteria of Lactobacillus rhamnosus and Bifidobacterium is 1:3; Aspergillus oryzae is inoculated at an inoculation rate of 3% by volume; and the mixed lactic acid bacteria is inoculated at an inoculation rate of 5% by volume;

[0055] 2) Take parasitic herb, Dipsacus asper, white peony root, Chuanxiong and donkey-hide gelatin, soak them in warm water, filter and set aside the filtrate; decoct the residue with water, filter and combine the above filtrates to concentrate to a density of 1.23 g / mL and set aside;

[0056] 3) Take Chinese Angelica, Scutellaria, and Atractylodes macrocephala, add ethanol to extract by reflux, filter, add ethanol to the filtrate to make the alcohol content reach 70%, let stand overnight, filter, and concentrate under reduced pressure to a relative density of 1.25 g / mL;

[0057] 4) After the extracts of steps 1), 2) and 3) are combined and mixed, common pharmaceutical excipients are added to prepare a conventional preparation.

[0058] Preparation of Example 3 Granules:

[0059] Formula: Cuscuta 100g, Morus alba 80g, Dipsacus asper 50g

[0060] White Peony 80g Angelica 80g Scutellaria 60g

[0061] Chuanxiong 60g Atractylodes 40g Donkey-hide gelatin 20g

[0062] The preparation method is the same as Example 1.

[0063] Example 4 Preparation of granules:

[0064] Formula: Cuscuta chinensis 60g, Morus alba 50g, Dipsacus asper 50g

[0065] White Peony 50g Angelica 50g Scutellaria 60g

[0066] Chuanxiong 50g Atractylodes 40g Donkey-hide gelatin 40g

[0067] The preparation method is the same as Example 1.

[0068] Example 5 Preparation of granules:

[0069] Formula: Cuscuta 80g, Morus alba 60g, Dipsacus asper 50g

[0070] White Peony 60g Angelica 60g Scutellaria 50g

[0071] Chuanxiong 60g Atractylodes 50g Donkey-hide gelatin 40g

[0072] The preparation method is the same as Example 1.

[0073] Preparation of Example 6 Granules:

[0074] Formula: Cuscuta 100g, Morus alba 60g, Dipsacus asper 80g

[0075] White Peony 60g Angelica 60g Scutellaria 80g

[0076] Chuanxiong 80g Atractylodes 60g Donkey-hide gelatin 20g

[0077] The preparation method is the same as Example 1.

[0078] Preparation of Comparative Example 1 Granules:

[0079] Formula: Cuscuta 120g, Morus alba 60g, Dipsacus asper 60g

[0080] White Peony 60g Angelica 60g Scutellaria 60g

[0081] Chuanxiong 60g Atractylodes macrocephala 30g Donkey hide gelatin 10g

[0082] Preparation method:

[0083] Accurately weigh each raw medicinal material according to the above weight portion, extract the effective ingredients according to the "water extraction-separation-concentration-drying" steps, add conventional medicinal excipients, and prepare the above Chinese medicine formula into formulated granules according to the conventional method according to the standard granulation method.

[0084] Preparation of Comparative Example 2 Granules:

[0085] Formula: Cuscuta 20g, Morus alba 60g, Dipsacus asper 60g

[0086] White Peony 30g Angelica 60g Scutellaria 60g

[0087] Chuanxiong 60g Atractylodes macrocephala 5g Donkey hide gelatin 30g

[0088] The preparation method is the same as Example 1.

[0089] Preparation of Comparative Example 3 Granules:

[0090] Formula: Cuscuta 80g, Morus alba 60g, Dipsacus asper 50g

[0091] White peony root 30g Chinese angelica root 30g Scutellaria baicalensis 30g

[0092] Chuanxiong 30g Atractylodes 5g

[0093] The preparation method is the same as Example 1.

[0094] Preparation of Comparative Example 4 Granules:

[0095] Formula: Cuscuta 80g, Morus alba 30g, Dipsacus asper 30g

[0096] White peony root 40g Codonopsis pilosula 60g Scutellaria baicalensis 60g

[0097] 60g wolfberry, 50g atractylodes, 20g poria

[0098] The preparation method is the same as Example 1.

[0099] Preparation of Comparative Example 5 Granules:

[0100] Formula: Cuscuta 80g, Morus alba 60g, Dipsacus asper 50g

[0101] 60g cinnamon twig, 20g Eclipta prostrata, 30g Ligustrum lucidum

[0102] Chuanxiong 60g Atractylodes 50g

[0103] The preparation method is the same as Example 1.

[0104] Preparation of Comparative Example 6 Granules:

[0105] Formula: Cuscuta 80g, Morus alba 60g, Dipsacus asper 50g

[0106] White Peony 60g Angelica 60g Scutellaria 60g

[0107] Chuanxiong 60g Atractylodes 50g

[0108] Preparation method:

[0109] Accurately weigh each raw medicinal material according to the above weight, add 6 times the amount of water, decoct for 3 times, decoct for 1 hour each time, combine the decoctions, concentrate, dry, and prepare into granules according to the conventional method.

[0110] Preparation of Comparative Example 7 Granules:

[0111] Formula: Cuscuta 80g, Morus alba 60g, Dipsacus asper 50g

[0112] White Peony 60g Angelica 60g Scutellaria 60g

[0113] Chuanxiong 60g Atractylodes 50g Donkey-hide gelatin 30g

[0114] Preparation method:

[0115] 1) Take Cuscuta, Morus alba, Dipsacus asper, Paeonia lactiflora, Rhizoma Chuanxiong and donkey-hide gelatin, soak them in warm water, filter and set aside the filtrate; decoct the residues with water, filter and combine the above filtrates to concentrate to a density of 1.18 g / mL and set aside;

[0116] 3) Take Chinese Angelica, Scutellaria, and Atractylodes macrocephala, add ethanol to extract by reflux, filter, add ethanol to the filtrate to make the alcohol content reach 70%, let stand overnight, filter, and concentrate under reduced pressure to a relative density of 1.25 g / mL;

[0117] 3) After the extracts of step 1) and step 2) are combined and mixed, common pharmaceutical excipients are added to prepare a conventional preparation.

[0118] Verification Example 1: Intervention effect of the Chinese medicine composition of the present invention on mice with recurrent spontaneous abortion

[0119] 1. Materials and instruments

[0120] 1.1 Experimental Animals:

[0121] Select SPF-grade healthy mice: select female CBA / J mice, male DBA / 2, and Balb / c mice, 8 weeks old, with a body weight of 20±2g.

[0122] 1.2 Drugs and preparations:

[0123] Commercially available enteric-coated aspirin tablets, granules of Examples 1-3, and granules of Comparative Examples 1-3.

[0124] 2. Experimental methods and test indicators

[0125] 2.1 Establishment of animal model

[0126] Female CBA / J mice were mated with Balb / c mice at a ratio of 2:1 to establish a normal pregnancy mouse model. Female CBC / J mice were mated with male DBA / 2 mice at a ratio of 2:1 to establish a recurrent abortion mouse model. The vagina of the female mice was checked every morning. If a vaginal plug was found, the model was successfully established and counted as the first day of pregnancy.

[0127] 2.2 Grouping and Dosing

[0128] Eight normal pregnant mice with successful modeling were selected as the normal group.

[0129] The recurrent abortion mice with successful modeling were randomly divided into a model group, a positive control group, Example 1 group, Example 2 group, Example 3 group, Comparative Example 1 group, Comparative Example 2 group, and Comparative Example 3 group, with 8 mice in each group.

[0130] The normal group and the model group were gavaged with equal volume of distilled water. The positive control group was gavaged with 0.11 mg / 10 g of aspirin enteric-coated tablets water solvent every day, and each example group and the comparative example group were gavaged with 12.35 g / Kg.

[0131] Each group of mice was given medication once a day starting from the first day of pregnancy.

[0132] 3. Detection indicators

[0133] 3.1 Changes in serum IGFBP-1 levels in each group of mice

[0134] All mice in each group were killed 12 hours after intragastric administration on the 8th day of pregnancy, and the uterine decidua tissue of mice was collected. The serum was collected after natural coagulation and centrifugation of fundus venous blood, and the level of IGFBP-1 in decidua tissue was detected by ELISA.

[0135] 3.2 Detection of mouse endometrial stromal cell senescence

[0136] The mice in each group were killed 12 hours after intragastric administration on the 8th day of pregnancy, and the decidual tissues were obtained. The senescence level of endometrial stromal cells was detected by senescence β-galactosidase staining kit.

[0137] 3.3 Calculation of mouse pregnancy absorption rate

[0138] After the mice in each group were killed, the uterus was removed, the endometrial stromal cells were measured, and the embryo absorption rate was calculated.

[0139] Embryo absorption rate = number of absorbed embryos / (number of absorbed embryos + number of live embryos) × %

[0140] 3.3 Statistical methods

[0141] SPSS22.0 software was used for data analysis.

[0142] 4. Results

[0143] 4.1 Changes in serum IGFBP-1 levels in each group of mice

[0144] The test results of each experimental group were analyzed using SPSS22.0 software. Figure 1 As shown in the figure, it can be seen that the IGFBP-1 content of the model group is significantly lower than that of the other groups, and there is no significant difference between the values ​​of each embodiment group and the normal group, the positive drug control group, and the comparative embodiment 1 group, among which the value of the embodiment 1 group is the highest, but the data of the comparative embodiment 2 group and the comparative embodiment 3 group are significantly lower than those of the experimental groups, and there is no significant difference with the model group.

[0145] 4.2 Changes in senescence of endometrial stromal cells in each group of mice

[0146] Figure 2 Some senescent cells show β-galactosidase staining after staining. Figure 3 The effect of the Chinese medicine composition of the present invention on the aging of endometrial stromal cells in mice with recurrent abortion. From the experimental results, it can be seen that the number of untreated β-galactosidase-positive cells in the recurrent abortion model group is significantly increased compared with the normal control group, indicating that the level of cell aging is significantly increased. After 8 days of treatment with the positive control drug, the Chinese medicine composition of the present invention, and the Chinese medicine of the comparative example 1 group, the number of endometrial β-galactosidase-positive cells is significantly reduced; while the comparative example 2 group and the comparative example 3 group have no significant difference with the model group. It can be seen that the Chinese medicine composition of the present invention can reduce the aging of endometrial stromal cells in mice with recurrent abortion.

[0147] 4.3 Embryo absorption rate of mice in each group

[0148] Figure 4 The effect of the Chinese medicine composition of the present invention on the uterine embryo absorption rate of mice with recurrent abortion. Figure 3 The experimental results show that the embryo absorption rate of the normal group of mice is only about 5%. After 8 days of treatment with the positive control drug, the Chinese medicine composition of the present invention, and the Chinese medicine of the comparative example 1 group, the embryo absorption rate can be restored to about 12%; while the comparative example 2 group and the comparative example 3 group have no significant difference from the model group. It can be seen that the Chinese medicine composition of the present invention can significantly reduce the embryo absorption rate and is effective in treating recurrent abortion.

Claims

1. A Chinese medicine preparation containing a fermented extract of Cuscuta australis, characterized in that: The fermented extract of Cuscuta seeds is obtained by fermenting Cuscuta seeds with Aspergillus oryzae and mixed lactic acid bacteria, wherein the mixed lactic acid bacteria is a mixed bacteria of Lactobacillus rhamnosus and Bifidobacterium; The preparation method of the Chinese medicine preparation is: 1) Cuscuta fermentation extraction: crush the Cuscuta, add it into MRS liquid culture medium, mix it evenly, sterilize it, inoculate Aspergillus oryzae and mixed lactic acid bacteria into MRS liquid culture medium in sequence, and ferment it; wherein, the inoculation interval of Aspergillus oryzae and mixed lactic acid bacteria is 12-36h, the fermentation time is 4-6d, and the fermentation temperature is: 35-38°C; after the fermentation is completed, centrifuge, filter, and take the supernatant for use; 2) Take parasitic herb, Dipsacus asper, white peony root, Chuanxiong and donkey-hide gelatin, soak them in warm water, filter and set aside the filtrate; decoct the residue with water, filter and combine the above filtrates to concentrate to a density of 1.23-1.28 g / mL and set aside; 3) Take Chinese Angelica, Scutellaria, and Atractylodes macrocephala, add ethanol to extract by reflux, filter, add ethanol to the filtrate to make the alcohol content reach 70%, let stand overnight, filter, and concentrate under reduced pressure to a relative density of 1.22-1.25 g / mL; 4) After the extracts of steps 1), 2) and 3) are combined and mixed, common pharmaceutical excipients are added to prepare a conventional preparation.

2. The Chinese medicine preparation according to claim 1, characterized in that: In terms of weight ratio, the raw materials in the Chinese medicine preparation are composed of: Cuscuta chinensis 60-100 parts by weight Loranthus parasiticus 50-80 parts by weight Dipsacus asper 50-80 parts by weight 50-80 weight parts of white peony root 50-80 weight parts of angelica root 50-80 weight parts of scutellaria root 50-80 weight parts 50-80 weight parts of Chuanxiong, 40-60 weight parts of Atractylodes macrocephala, 20-40g of donkey-hide gelatin.

3. The Chinese medicine preparation according to claim 1, characterized in that: The weight ratio of the mixed bacteria of Lactobacillus rhamnosus and Bifidobacterium is 1:1-3.

4. The Chinese medicine preparation according to claim 1, characterized in that: In the step (1), Aspergillus oryzae is inoculated at an inoculum volume ratio of 3%-7%.

5. The Chinese medicine preparation according to claim 1, characterized in that: In the step (1), mixed lactic acid bacteria are inoculated at an inoculation rate of 2% to 5% by volume.

6. The Chinese medicine preparation according to claim 1, characterized in that: The extraction step in step (2) is repeated three times, with the first decoction being 2 hours, the second decoction being 1 hour, and the third decoction being 0.5 hours.

7. The Chinese medicine preparation according to claim 1, characterized in that: The Chinese medicine preparation is a conventional oral preparation.

8. Use of the Chinese medicine composition according to claim 1 in the preparation of a drug for inhibiting the aging of endometrial stromal cells and reducing embryo absorption rate.

9. The use according to claim 8, characterized in that The Chinese medicine composition is used in preparing medicine for preventing and treating recurrent spontaneous abortion.

10. The use according to claim 9, characterized in that The recurrent spontaneous abortion is unexplained recurrent spontaneous abortion.

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