Chrysophyceae polysaccharide extraction equipment capable of optimizing polysaccharide extraction efficiency and use method of Chrysophyceae polysaccharide extraction equipment

Abstract

The invention relates to the related technical field of biological sugar refining, and discloses chrysophyceae polysaccharide extraction equipment capable of optimizing polysaccharide extraction efficiency and a use method thereof.The chrysophyceae polysaccharide extraction equipment comprises a round hexagonal supporting frame, a placement ring is arranged at the upper end of the round hexagonal supporting frame, and a glass jar lower body is installed on the surface of the placement ring; the glass jar comprises a glass jar lower body, a glass jar upper body is arranged above the glass jar lower body, a motor shell is fixedly installed on the upper surface of the glass jar upper body in a threaded mode, a driving motor is fixedly arranged in the motor shell, and the output end of the driving motor is rotationally connected with a driving shaft. When polysaccharide stored in chrysophyceae cells encounters precipitation and caking conditions, the precipitation blocks can enter the water inlet cavity through the first water inlet and the damping universal base and physically collide with the protruding blocks, large or complete precipitation blocks are broken into small particles, and the contact area of the polysaccharide and a solvent is increased.

Description

Technical Field

[0001] The present invention relates to the technical field related to microbial sugar production, and in particular to a golden algae polysaccharide extraction device for optimizing polysaccharide extraction efficiency and a method for using the same. Background Art

[0002] As a kind of microalgae rich in polysaccharides, golden algae has rich biological resources. By using golden algae fermentation broth to extract polysaccharides, it is possible to achieve efficient utilization of golden algae resources, improve the yield and purity of polysaccharides, and extract polysaccharides from golden algae fermentation broth has a wider application prospect, which can be applied to food, health products, medicine and cosmetics, and promote the industrial application and market expansion of golden algae polysaccharides. However, the existing golden algae polysaccharide extraction equipment still has the following defects: During the polysaccharide extraction and purification process, the solubility and interaction mode of polysaccharides with other substances will be different under different ethanol concentration conditions, which may lead to the formation of precipitates. Traditional golden algae polysaccharide extraction equipment can only use traditional mechanical stirring rods, and traditional mechanical stirring rods cannot effectively disperse and dissolve the precipitates evenly, resulting in some precipitates that cannot be completely processed. In addition, traditional mechanical stirring rods usually consume a lot of electricity for stirring operations, thereby increasing energy consumption and costs. The stirring rod or stirring blade inside the existing golden algae polysaccharide extraction equipment can only perform simple stirring actions and cannot cooperate with other equipment for effective auxiliary operations. In addition, the existing stirring rod or stirring blade design is relatively complex and difficult to clean, and it is easy to leave residual substances or bacteria, affecting the next use or product purity. Most of the existing golden algae polysaccharide extraction tanks use metal tanks, and metal tanks may have certain strength and durability, but when used in golden algae polysaccharide extraction equipment, they are prone to corrosion, reactivity, difficulty in cleaning and weight. In addition, some metal tanks adopt an integrated design. The integrated tank design usually has many hard-to-reach areas such as corners and internal corners, which are difficult to clean and easy to breed bacteria and affect product purity and product quality. Summary of the invention

[0003] The object of the present invention is to provide a golden algae polysaccharide extraction device and a method of using the same for optimizing the polysaccharide extraction efficiency, so as to overcome the above-mentioned defects in the prior art.

[0004] The present invention is achieved through the following technical solutions.

[0005] The present invention provides a golden algae polysaccharide extraction device for optimizing polysaccharide extraction efficiency and a method for using the same, comprising a circular hexagonal support frame, a placement ring being provided at the upper end of the circular hexagonal support frame, a glass cylinder lower body being installed on the surface of the placement ring, a glass cylinder upper body being provided above the glass cylinder lower body, a motor housing being fixedly installed on the upper surface of the glass cylinder upper body with threads, a driving motor being fixedly provided inside the motor housing, a driving shaft being rotatably connected to the output end of the driving motor, a concentration probe being fixedly installed at the lower end of the driving shaft, and primary cross bars being symmetrically provided on the surface of the lower section of the driving shaft, a crushing device being fixedly installed on the upper surface of the symmetrically provided primary cross bars, a secondary cross bar and a tertiary cross bar being provided above the crushing device, an arc-shaped stirring blade being fixedly connected between the secondary cross bar and the threaded layer, three vertical stirring rods being arranged in an array on the upper surface of the secondary cross bar, and the vertical stirring rod being located inside the arc-shaped stirring blade Side; the crushing device includes a square crushing shell, a water inlet chamber is provided inside the square crushing shell, an arc-shaped crushing shell is provided at the rear end of the square crushing shell, the arc-shaped crushing shell and the square crushing shell are fixedly connected by an arc-shaped baffle, and end blocks are symmetrically provided at both ends of the upper surface of the arc-shaped crushing shell, and the symmetrical end blocks are provided with slide grooves opening inwardly, an arc-shaped sliding cover is slidably installed in the slide groove, and sliding blocks are symmetrically provided on the lower surfaces of both ends of the arc-shaped sliding cover, the sliding blocks are slidably installed in the slide groove, and a connecting rod is fixedly installed on the outer surface of the arc-shaped sliding cover, a fixing block is fixedly installed on the upper end of the connecting rod, the fixing block is fixedly connected with the formation, and a lower end block is fixedly connected below the square crushing shell, the lower end block is fixed to the primary cross bar, and a second water inlet is provided at the front end opening of the lower end block, and the second water inlet is connected with the water inlet chamber.

[0006] A further technical solution is that a plurality of large-sized crushing balls are fixedly installed on the inner wall of the lower surface of the arc-shaped sliding cover and the inner wall of the upper surface of the arc-shaped crushing shell, a crushing groove is provided between the large-sized crushing balls on both sides, the crushing groove is connected with the water inlet chamber, and a crushed crystal discharge outlet is provided on the upper surface of the end of the arc-shaped crushing shell, and a plurality of discharge through holes are provided on the surface of the crushed crystal discharge outlet.

[0007] According to a further technical solution, a first water inlet is provided at the front end of the square crushing shell, and vertical through holes are provided on both sides of the square crushing shell, and the vertical through holes are connected to the inside of the water inlet cavity.

[0008] A further technical solution is that a plurality of reinforcing lock buckles are provided in an annular array on the upper surface of the glass cylinder upper body; and a plurality of lower end blocks are provided in an annular array on the lower surface of the placement ring, and the lower end blocks form a socket connection with the placement ring and form secondary reinforcement for the glass cylinder upper body and the glass cylinder lower body.

[0009] According to a further technical solution, a circular inner arc hole is provided at the bottom of the inner wall of the lower body of the glass cylinder, and a manual liquid drainage component is fixedly installed at the bottom of the lower body of the glass cylinder, and the manual liquid drainage component is connected to the circular inner arc hole.

[0010] According to a further technical solution, a stirring chamber is provided inside the upper body of the glass cylinder, a sedimentation chamber is provided inside the lower body of the glass cylinder, a threaded layer is provided on the upper section of the lower body of the glass cylinder, and the stirring chamber is threadedly connected to the threaded layer.

[0011] According to a further technical solution, an ethanol concentration detection gauge is fixedly mounted on the upper surface of the upper body of the glass cylinder, the ethanol concentration detection gauge is connected to the concentration probe, and a liquid filling port is provided on the left side of the ethanol concentration detection gauge, the liquid filling port is connected to the interior of the stirring chamber.

[0012] According to a further technical solution, six support rods are arranged in a circular array on the lower surface of the placement ring, and a shock-absorbing universal base is fixedly installed at the bottom of each support rod.

[0013] According to a further technical solution, a plurality of protrusions are provided on the inner wall of the water inlet chamber; and a plurality of small crushing balls are provided inside the arc-shaped baffle.

[0014] According to a further technical solution, a switch button is installed on the upper surface of the motor housing, and the switch button can be divided into a primary button or a secondary button.

[0015] A further technical solution is a method for extracting golden algae polysaccharides, characterized in that it comprises the following steps: S1: firstly, heat-treating the golden algae fermentation liquid in a container at 60°C for 10 minutes; after completion, rapidly cooling the solution to room temperature; S2: Then, a Buchner funnel and a bottom container are set up and connected to a vacuum pump to achieve reduced pressure filtration; two medium-speed qualitative filter papers are laid on the bottom of the Buchner funnel, diatomaceous earth with a thickness of about 1-2 cm is evenly laid on the filter paper, and an appropriate amount of pure water is added to fully moisten the filter paper and diatomaceous earth; the heat-treated fermentation liquid is slowly added to the Buchner funnel, and the vacuum pump is started to start reduced pressure filtration. During the filtration process, if the filtration speed is found to slow down, the algae mud blocking the surface of the diatomaceous earth should be promptly removed with a scraper to maintain the filtration speed; when the fermentation liquid has all passed through the filter cake and has been filtered clean, the vacuum pump is turned off and the filtrate is removed; S3: Then, 1% (mass fraction) of activated carbon powder is added to the filtrate, and mechanical stirring is used at room temperature at a speed of 400 revolutions per minute (rpm) for 0.5 hours; this high-speed stirring helps the activated carbon to fully contact with the pigment in the filtrate and effectively adsorb it, thereby achieving a decolorization effect. After the decolorization process is completed, observe the color change of the filtrate; if the filtrate has become clear enough, it means that the decolorization effect is good; if there is still color remaining, it may be necessary to increase the amount of activated carbon or extend the stirring time; S4: Assemble the Buchner funnel and the container, place two medium-speed qualitative filter papers in the Buchner funnel, slowly pour the decolorized golden algae fermentation liquid (containing activated carbon at this time) into the Buchner funnel, turn on the vacuum pump, and use the reduced pressure filtration method to make the liquid pass through the filter paper, so as to leave the activated carbon and other solid impurities on the filter paper; S5: Then connect the Büchner funnel to the container for receiving the filtrate to ensure that the entire system is sealed and the filtration operation can be carried out normally; place a 5μm microporous filter membrane in the Büchner funnel; this filter membrane has a larger pore size and is mainly used to remove larger particles; pour the decolorized filtrate into the Büchner funnel equipped with the 5μm filter membrane, turn on the vacuum pump for filtration, and when the filtration of the 5μm filter membrane is completed, replace it with a 0.22μm microporous filter membrane and continue to filter the remaining decolorized filtrate; this filter membrane has a smaller pore size and can remove smaller particles; during the filtration process, if the filtration speed is found to be significantly slower, this may be because the filter membrane is blocked; at this time, a new filter membrane should be replaced to maintain the filtration efficiency; when all the decolorized filtrate is filtered through the 0.22μm filter membrane, turn off the vacuum pump and collect the filtrate; S6: First pour the filtrate into the lower body of the glass cylinder and then lock the upper body of the glass cylinder upside down. After locking, slowly add 95% ethanol equivalent to four times its volume to the filtrate, and turn on the switch button to start the drive motor to quickly stir the internal solution to ensure that the ethanol and the filtrate are fully mixed; after adding ethanol, the ethanol concentration in the solution should reach 76%, then open the manual drainage component to allow the solution to flow into the container, and after the solution has completely flowed into the container, transfer it to a refrigerator or freezer; let the solution stand at a temperature of -20℃ to 0℃ for 6 to 8 hours; preferably, alcohol precipitation at -20℃, because lower temperatures are conducive to the aggregation and precipitation of polysaccharide molecules; after sufficient alcohol precipitation time, the polysaccharide precipitates from the solution; at this time, the supernatant mainly contains unprecipitated proteins and other soluble substances; S7: Pour off the supernatant in the alcohol-precipitated liquid, leaving only the sediment at the bottom; transfer the sediment to centrifuge tubes, and ensure that the weight of the sediment in all centrifuge tubes is roughly equal to maintain balance during the centrifugation process; unbalanced centrifuge tubes may cause the centrifuge to vibrate, thereby affecting the centrifugation effect. Place the centrifuge tube containing the sediment in the centrifuge, set the centrifugation time to 8 minutes, and the centrifugal force to 4000g. The purpose of centrifugation is to further separate the solids and possible residual liquid in the sediment; after the centrifugation is completed, open the centrifuge tube, pour off the supernatant, and collect the crude polysaccharide wet product left at the bottom of the centrifuge tube; S8: Add an appropriate amount of pure water to the crude polysaccharide wet product obtained by centrifugation to fully dissolve the polysaccharide. The concentration of the solution should be adjusted to 40g / l. Use medium-speed qualitative filter paper to preliminarily filter the solution to remove larger particles and impurities. The filtered solution is further filtered through a 0.22μm microporous filter membrane to remove smaller particles and activated carbon that may not be completely removed. During the filtration process, some sticky gray-black colloidal solids may remain on the filter paper, which are mainly activated carbon and protein denatured by alcohol precipitation. After the filtration is completed, check the clarity of the filtrate. If the filtrate is still not clear enough, further filtration or purification steps may be required. S9: Add four times the volume of 95% ethanol to the filtrate for alcohol precipitation, and repeat step 6; S10: Pour off the supernatant of the precipitate (polysaccharide) after the secondary alcohol precipitation treatment, divide the precipitate into centrifuge tubes and put them into a centrifuge, centrifuge at a centrifugal force of 4000g, usually for 8 minutes, further compress the precipitate and remove the ethanol and water that may remain in the precipitate; after the centrifugation, collect the precipitate, add an appropriate amount of 95% ethanol to mash it, put the mashed precipitate into a vortex oscillator and oscillate for about 1 minute, which helps the ethanol to penetrate the precipitate more deeply, thereby removing more impurities; put the washed and oscillated precipitate into the centrifuge again, and centrifuge it at a centrifugal force of 4000g for 8 minutes; after the centrifugation, pour off the supernatant and retain the washed polysaccharide precipitate; the precipitate obtained at this time is the refined crude polysaccharide wet product; S11: The wet crude polysaccharide product is freeze-dried overnight to obtain a dry refined product; S12: Grind the freeze-dried refined product with a mortar and pass it through an 80-mesh sieve to collect the powder; (Quality inspection: pure water can be used to prepare a 10g / l concentration solution to observe whether it is clear and transparent and whether there are insoluble substances; in this state, the solubility of the product in water is greater than 80g / l and ≤100g / l.).

[0016] Beneficial effects of the present invention: In the present invention, a golden algae polysaccharide extraction device and a method for using the same for optimizing polysaccharide extraction efficiency can be used. The crushing device can make the polysaccharides stored in the golden algae cells encounter precipitation and agglomeration. The agglomerated precipitate blocks can enter the water inlet chamber through the first water inlet and the shock-absorbing universal base and form a physical collision with the protrusion to break the large or complete precipitate blocks into tiny particles, thereby increasing the contact area between the polysaccharide and the solvent, which is beneficial for the solvent to penetrate into the precipitate blocks, and can improve the dissolution efficiency and polysaccharide yield in the subsequent extraction process. When the precipitate blocks are accompanied by the rotating centrifugal force generated by the driving motor, they will encounter the arc baffle and be blocked by the arc baffle to enter the arc crushing shell. After entering the arc crushing shell, the large crushing balls provided on the inner wall of the arc crushing shell will violently collide, roll and rub with the precipitate blocks, and exert a continuous and all-round grinding effect on the golden algae cells and the polysaccharide precipitate blocks therein; and when the arc stirring blades are subjected to reverse thrust under the action of the water body, the fixed blocks installed on the back thereof will drive the sliders symmetrically arranged at the bottom of the arc sliding cover to slide slightly in the slide groove through the connecting rod. This tiny sliding process allows the large crushing balls embedded in the lower surface of the arc-shaped sliding cover and the large crushing balls on the surface of the arc-shaped crushing shell to interact with each other in an interlaced grinding manner to achieve a grinding effect, which will help to obtain crushed products with smaller particle sizes, increase the contact area between polysaccharides and solvents, and improve the dissolution efficiency and polysaccharide yield in the subsequent extraction process. It can further reduce clogging of the crushed fine particles during solid-liquid separation processes such as filtration and centrifugation, improve separation efficiency, and increase the amount of polysaccharides released from cells or sediment blocks, thereby improving the polysaccharide yield of the entire extraction process and ensuring the effective use of resources and maximization of economic benefits.

[0017] In the present invention, a golden algae polysaccharide extraction device for optimizing polysaccharide extraction efficiency and a method for using the same are provided with an arc-shaped stirring blade above the secondary cross bar. The strong vortex generated during rotation forms a strong shear force and an upward flow, which effectively overcomes the natural sedimentation tendency of the precipitate particles in the solvent and ensures that they are continuously suspended in the stirring area. It can also ensure sufficient mixing and uniform dispersion between the broken precipitate and the solvent, so that each precipitate particle can be surrounded by sufficient solvent and then quickly and fully dissolved, and this also avoids the problem of decreased dissolution rate due to excessive concentration of a certain piece. Secondly, the close cooperation between the arc-shaped stirring blade and the broken crystal discharge port ensures that the newly generated fine precipitate particles are drawn into the high-efficiency stirring environment the moment they leave the crushing device, achieving immediate and comprehensive contact with the solvent. Moreover, this dissolution effect greatly shortens the process from crushing to dissolution of the precipitate particles, accelerates the dissolution process of the precipitate, and significantly improves the dissolution rate of golden algae polysaccharides. Finally, the arc-shaped stirring blade generates heat during the stirring process, and evenly distributes the heat through the arc-shaped surface of the arc-shaped stirring blade, which is conducive to maintaining the stability of the solution temperature and reducing the influence of thermal effects on the activity of polysaccharides, thereby ensuring the thermodynamic stability of the final solution.

[0018] In the present invention, a golden algae polysaccharide extraction device and a method of using the same for optimizing polysaccharide extraction efficiency adopt a spiral split design for the glass cylinder upper body and the glass cylinder lower body, so that the various components of the device can be installed, disassembled and replaced by themselves, which greatly facilitates the daily maintenance of the equipment; and the transparent glass material used on the surface of the glass cylinder upper body and the glass cylinder lower body allows the operator to intuitively and clearly observe the entire extraction process in the tank, including the crushing of the sediment block, the stirring state, the solution color, the degree of turbidity and other key information, and golden algae polysaccharides are generally less sensitive to light, and will not cause unnecessary light-induced degradation or denaturation when using a reaction tank made of transparent glass. In addition, the glass material has good chemical inertness and is not easy to react with the solvent or the substance to be extracted, ensuring the purity of the extraction process and the integrity of the extract. Moreover, the surface of the glass material is smooth, with no dead corners where dirt can accumulate, and it is easy to clean, which can effectively prevent cross-contamination. The manual drainage assembly fixedly installed at the bottom of the lower body of the glass cylinder can use gravity to naturally discharge the extracted polysaccharide solution. No additional power equipment is required to achieve an efficient and smooth discharge process, and the extract can be quickly transferred to subsequent concentration, purification or drying processes, which simplifies the process flow and improves production efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] In order to more clearly illustrate the embodiments of the invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0020] The present invention is further described below in conjunction with the accompanying drawings and embodiments.

[0021] Figure 1 It is a schematic diagram of the overall structure of the present invention; Figure 2 It is a schematic diagram of the overall front view structure of the present invention; Figure 3 It is a front cross-sectional structural schematic diagram of the present invention; Figure 4 It is a schematic diagram of a top cross-sectional structure of the present invention; Figure 5 It is a schematic diagram of the overall side view structure of the lower body 13 of the glass cylinder in the present invention; Figure 6 1 is a schematic diagram of the top view of the lower body 13 of the glass cylinder in the present invention; Figure 7 It is a schematic diagram of the overall appearance structure of the motor housing 14 in the present invention; Figure 8 Schematic diagram of the overall appearance structure of the crushing device 30 of the present invention; Fig. 9 is a schematic diagram of the side cross-sectional structure of the crushing device 30 of the present invention; Fig.10 1 is a schematic diagram of the top view of the crushing device 30 of the present invention; DETAILED DESCRIPTION

[0022] Combine the following Figure 1-10 The present invention is described in detail. For the convenience of description, the directions mentioned below are defined as follows: the up, down, left, right, front, and back directions mentioned below are the same as Figure 1 The up-down, left-right, front-back directions of the projection relationship itself are consistent.

[0023] In combination with the accompanying drawings 1-10, a golden algae polysaccharide extraction device for optimizing the polysaccharide extraction efficiency and a method of using the same include: a circular hexagonal support frame 12, a placement ring 47 is provided at the upper end of the circular hexagonal support frame 12, a glass cylinder lower body 13 is installed on the surface of the placement ring 47, a glass cylinder upper body 11 is provided above the glass cylinder lower body 13, a motor housing 14 is threadedly fixedly installed on the upper surface of the glass cylinder upper body 11, a drive motor 20 is fixedly provided inside the motor housing 14, a drive shaft 22 is rotatably connected to the output end of the drive motor 20, and the drive A concentration probe 51 is fixedly installed at the lower end of the shaft 22, and a primary cross bar 25 is symmetrically installed on the lower surface of the drive shaft 22. A crushing device 30 is fixedly installed on the upper surface of the symmetrically installed primary cross bar 25. A secondary cross bar 28 and a tertiary cross bar 46 are arranged above the crushing device 30. An arc-shaped stirring blade 29 is fixedly connected between the secondary cross bar 28 and the threaded layer 26. Three vertical stirring rods 42 are arranged in an array on the upper surface of the secondary cross bar 28. The vertical stirring rod 42 is located on the inner side of the arc-shaped stirring blade 29. The crushing device 30 includes a square crushing shell 35, a water inlet chamber 49 is provided inside the square crushing shell 35, an arc-shaped crushing shell 37 is provided at the rear end of the square crushing shell 35, the arc-shaped crushing shell 37 and the square crushing shell 35 are fixedly connected by an arc-shaped baffle 36, and end blocks 59 are symmetrically provided at both ends of the upper surface of the arc-shaped crushing shell 37, and the symmetrical end blocks 59 are provided with a slide groove 60 with an opening inward, and an arc-shaped sliding cover 54 is slidably installed in the slide groove 60, and the lower surfaces of the two ends of the arc-shaped sliding cover 54 are symmetrically provided. There is a slider 61, which is slidably installed inside the slide groove 60, and a connecting rod 55 is fixedly installed on the outer surface of the arc-shaped sliding cover 54, and a fixed block 56 is fixedly installed on the upper end of the connecting rod 55, and the fixed block 56 is fixedly connected with the 29, and a lower end block 48 is fixedly connected below the square crushing shell 35, and the lower end block 48 is fixed with the primary cross bar 25, and a second water inlet 34 is provided at the front end opening of the lower end block 48, and the second water inlet 34 is connected with the water inlet chamber 49.

[0024] Preferably, a plurality of large-sized crushing balls 38 are fixedly mounted on the inner wall of the lower surface of the arc-shaped sliding cover 54 and the inner wall of the upper surface of the arc-shaped crushing shell 37, a crushing groove 39 is provided between the large-sized crushing balls 38 on both sides, the crushing groove 39 is connected with the water inlet chamber 49, and a crushed crystal discharge outlet 40 is provided on the upper surface of the end of the arc-shaped crushing shell 37, and a plurality of discharge through holes 41 are provided on the surface of the crushed crystal discharge outlet 40.

[0025] Preferably, a first water inlet 31 is provided at the front end of the square crushing shell 35 , and vertical through holes 33 are provided on both sides of the square crushing shell 35 , and the vertical through holes 33 are communicated with the inside of the water inlet chamber 49 .

[0026] Preferably, a plurality of reinforcing lock buckles 17 are provided in an annular array on the upper surface of the glass cylinder upper body 11; and a plurality of lower end blocks 48 are provided in an annular array on the lower surface of the placement ring 47, and the lower end blocks 48 form a socket connection with the placement ring 47 and form secondary reinforcement for the glass cylinder upper body 11 and the glass cylinder lower body 13.

[0027] Preferably, a circular inner arc hole 27 is provided at the bottom of the inner wall of the glass cylinder lower body 13 , and a manual drainage component 18 is fixedly installed at the bottom of the glass cylinder lower body 13 , and the manual drainage component 18 is connected to the circular inner arc hole 27 .

[0028] Preferably, a stirring chamber 23 is provided inside the upper body 11 of the glass cylinder, a sedimentation chamber 24 is provided inside the lower body 13 of the glass cylinder, a threaded layer 26 is provided on the upper section of the lower body 13 of the glass cylinder, and the stirring chamber 23 and the threaded layer 26 form a threaded connection.

[0029] Preferably, an ethanol concentration detection gauge 16 is fixedly installed on the upper surface of the glass cylinder upper body 11, the ethanol concentration detection gauge 16 is connected to the concentration probe 51, and a liquid filling port 15 is provided on the left side of the ethanol concentration detection gauge 16, and the liquid filling port 15 is connected to the interior of the stirring chamber 23.

[0030] Preferably, six support rods 19 are provided in a circular array on the lower surface of the placement ring 47 , and a shock-absorbing universal base 43 is fixedly installed at the bottom of each support rod 19 .

[0031] Preferably, a plurality of protrusions 50 are provided on the inner wall of the water inlet cavity 49 ; and a plurality of small crushing balls 32 are provided inside the arc-shaped baffle 36 .

[0032] Preferably, a switch button 21 is installed on the upper surface of the motor housing 14 , and the switch button 21 can be divided into a primary button 52 or a secondary button 53 .

[0033] Preferably, a method for extracting golden algae polysaccharide is characterized by comprising the following steps: S1: firstly, heat-treating the golden algae fermentation liquid in a container at 60°C for 10 minutes; after completion, rapidly cooling the solution to room temperature; S2: Then, a Buchner funnel and a bottom container are set up and connected to a vacuum pump to achieve reduced pressure filtration; two medium-speed qualitative filter papers are laid on the bottom of the Buchner funnel, diatomaceous earth with a thickness of about 1-2 cm is evenly laid on the filter paper, and an appropriate amount of pure water is added to fully moisten the filter paper and diatomaceous earth; the heat-treated fermentation liquid is slowly added to the Buchner funnel, and the vacuum pump is started to start reduced pressure filtration. During the filtration process, if the filtration speed is found to slow down, the algae mud blocking the surface of the diatomaceous earth should be promptly removed with a scraper to maintain the filtration speed; when the fermentation liquid has all passed through the filter cake and has been filtered clean, the vacuum pump is turned off and the filtrate is removed; S3: Then, 1% (mass fraction) of activated carbon powder is added to the filtrate, and mechanical stirring is used at room temperature at a speed of 400 revolutions per minute (rpm) for 0.5 hours; this high-speed stirring helps the activated carbon to fully contact with the pigment in the filtrate and effectively adsorb it, thereby achieving a decolorization effect. After the decolorization process is completed, observe the color change of the filtrate; if the filtrate has become clear enough, it means that the decolorization effect is good; if there is still color remaining, it may be necessary to increase the amount of activated carbon or extend the stirring time; S4: Assemble the Buchner funnel and the container, place two medium-speed qualitative filter papers in the Buchner funnel, slowly pour the decolorized golden algae fermentation liquid (containing activated carbon at this time) into the Buchner funnel, turn on the vacuum pump, and use the reduced pressure filtration method to make the liquid pass through the filter paper, so as to leave the activated carbon and other solid impurities on the filter paper; S5: Then connect the Büchner funnel to the container for receiving the filtrate to ensure that the entire system is sealed and the filtration operation can be carried out normally; place a 5μm microporous filter membrane in the Büchner funnel; this filter membrane has a larger pore size and is mainly used to remove larger particles; pour the decolorized filtrate into the Büchner funnel equipped with the 5μm filter membrane, turn on the vacuum pump for filtration, and when the filtration of the 5μm filter membrane is completed, replace it with a 0.22μm microporous filter membrane and continue to filter the remaining decolorized filtrate; this filter membrane has a smaller pore size and can remove smaller particles; during the filtration process, if the filtration speed is found to be significantly slower, this may be because the filter membrane is blocked; at this time, a new filter membrane should be replaced to maintain the filtration efficiency; when all the decolorized filtrate is filtered through the 0.22μm filter membrane, turn off the vacuum pump and collect the filtrate; S6: First pour the filtrate into the lower body of the glass cylinder and then lock the upper body of the glass cylinder upside down. After locking, slowly add 95% ethanol equivalent to four times its volume to the filtrate, and turn on the switch button to start the drive motor to quickly stir the internal solution to ensure that the ethanol and the filtrate are fully mixed; after adding ethanol, the ethanol concentration in the solution should reach 76%, then open the manual drainage component to allow the solution to flow into the container, and after the solution has completely flowed into the container, transfer it to a refrigerator or freezer; let the solution stand at a temperature of -20℃ to 0℃ for 6 to 8 hours; preferably, alcohol precipitation at -20℃, because lower temperatures are conducive to the aggregation and precipitation of polysaccharide molecules; after sufficient alcohol precipitation time, the polysaccharide precipitates from the solution; at this time, the supernatant mainly contains unprecipitated proteins and other soluble substances; S7: Pour off the supernatant in the alcohol-precipitated liquid, leaving only the sediment at the bottom; transfer the sediment to centrifuge tubes, and ensure that the weight of the sediment in all centrifuge tubes is roughly equal to maintain balance during the centrifugation process; unbalanced centrifuge tubes may cause the centrifuge to vibrate, thereby affecting the centrifugation effect. Place the centrifuge tube containing the sediment in the centrifuge, set the centrifugation time to 8 minutes, and the centrifugal force to 4000g. The purpose of centrifugation is to further separate the solids and possible residual liquid in the sediment; after the centrifugation is completed, open the centrifuge tube, pour off the supernatant, and collect the crude polysaccharide wet product left at the bottom of the centrifuge tube; S8: Add an appropriate amount of pure water to the crude polysaccharide wet product obtained by centrifugation to fully dissolve the polysaccharide. The concentration of the solution should be adjusted to 40g / l. Use medium-speed qualitative filter paper to preliminarily filter the solution to remove larger particles and impurities. The filtered solution is further filtered through a 0.22μm microporous filter membrane to remove smaller particles and activated carbon that may not be completely removed. During the filtration process, some sticky gray-black colloidal solids may remain on the filter paper, which are mainly activated carbon and protein denatured by alcohol precipitation. After the filtration is completed, check the clarity of the filtrate. If the filtrate is still not clear enough, further filtration or purification steps may be required. S9: Add four times the volume of 95% ethanol to the filtrate for alcohol precipitation, and repeat step 6; S10: Pour off the supernatant of the precipitate (polysaccharide) after the secondary alcohol precipitation treatment, divide the precipitate into centrifuge tubes and put them into a centrifuge, centrifuge at a centrifugal force of 4000g, usually for 8 minutes, further compress the precipitate and remove the ethanol and water that may remain in the precipitate; after the centrifugation, collect the precipitate, add an appropriate amount of 95% ethanol to mash it, put the mashed precipitate into a vortex oscillator and oscillate for about 1 minute, which helps the ethanol to penetrate the precipitate more deeply, thereby removing more impurities; put the washed and oscillated precipitate into the centrifuge again, and centrifuge it at a centrifugal force of 4000g for 8 minutes; after the centrifugation, pour off the supernatant and retain the washed polysaccharide precipitate; the precipitate obtained at this time is the refined crude polysaccharide wet product; S11: The wet crude polysaccharide product is freeze-dried overnight to obtain a dry refined product; S12: Grind the freeze-dried refined product with a mortar and pass it through an 80-mesh sieve to collect the powder; (Quality inspection: pure water can be used to prepare a 10g / l concentration solution to observe whether it is clear and transparent and whether there are insoluble substances; in this state, the solubility of the product in water is greater than 80g / l and ≤100g / l.).

[0034] Specific use method and principle of the present invention: When using the present invention, the glass cylinder upper body 11 and the glass cylinder lower body 13 of the present invention are cleaned and dried. First, the shock-absorbing universal base 43 provided on the circular hexagonal support frame 12 is adjusted and placed flatly on the experimental table, and the bottom of the circular hexagonal support frame 12 is placed on the container used for alcohol precipitation. After drying, the lower body 13 of the glass cylinder is placed flat on the inner surface of the placement ring 47, and then the filtered filtrate is poured into the sedimentation chamber 24. Then, the upper body 11 of the glass cylinder is buckled on the top of the lower body 13 of the glass cylinder and spirally locked through the threaded layer 26. After the locking is completed, the reinforcing lock buckle 17 provided in the annular array on the upper surface of the upper body 11 of the glass cylinder and the lower end block 48 provided in the bottom array of the placement ring 47 are formed. The buckle is reinforced. After all the preparations are completed, the power supply of the device can be connected and turned on. Then, the first-level button 52 provided on the switch button 21 is pressed to rotate the drive shaft 22. When the drive shaft 22 rotates, the arc-shaped stirring blade 29 will quickly stir the filtrate inside the stirring chamber 23, and while stirring, start to insert the hole into the liquid filling port 15, and then slowly add four times the volume of 95% ethanol to the inside of the funnel. When 95% ethanol is added, the first-level button 52 is closed, and the second-level button 53 is pressed to quickly stir the inside of the filtrate. While stirring quickly, the crushing device 30 arranged under the arc-shaped stirring blade 29 will rotate clockwise and stir the inside of the stirring chamber 23. The sediment blocks provided at the bottom are quickly intercepted, and the sediment blocks to be intercepted enter the water inlet chamber 49 through the first water inlet 31, and collide with the protrusions 50 provided on the inner wall of the water inlet chamber 49 at high speed, and the high-speed rotation force generated by the drive shaft 22 forces the crushed sediment blocks to move backward. When they are pushed to the bottom, they are resisted and rebounded by the arc baffle 36 provided and enter the arc crushing shell 37. The sediment blocks are inside the arc crushing shell 37 and rub against the large crushing balls 38 symmetrically provided on the inner wall of the arc crushing shell 37. When the arc stirring blades 29 are subjected to reverse thrust under the action of the water body, the fixed blocks 56 installed on the back thereof drive the sliders 61 symmetrically arranged at the bottom of the arc sliding cover 54 in the slide groove 6 through the linkage rod 55. 0, this slight sliding process enables the large crushing balls 38 embedded on the lower surface of the arc-shaped sliding cover and the large crushing balls 38 on the surface of the arc-shaped crushing shell 37 to interact with each other in a staggered grinding manner, and ensures that 95% ethanol can wrap and dissolve the sediment block during high-speed rotation. When the dissolved solution is discharged to the outside through the discharge through hole 41, the arc-shaped stirring blade 29 provided after the discharge through hole 41 will quickly stir the dissolved solution and make the dissolution more complete, and when the arc-shaped stirring blade 29 stirs quickly, it will stir the inside of the sedimentation chamber 24 so that the sediment block will enter the inside of the crushing device 30 with the lift force to be crushed and dissolved, thereby improving the polysaccharide absorption rate of the entire extraction process.To ensure the effective use of resources and maximize economic benefits, observe the internal solution through the lower body 13 of the glass cylinder while stirring, and judge the degree of solution dissolution with the help of the ethanol concentration detection table 16. After the experimental extraction effect is achieved, the equipment can be turned off, and then the manual drainage component 18 can be slowly opened to allow the solution to flow into the container. When the solution is lower than the top of the lower body 13 of the glass cylinder, the unlocking placement ring 47 can be unlocked and disassembled into separate parts by rotating the upper body 11 of the glass cylinder. Finally, the scraper is used to scrape the internal surface of the precipitation chamber 24. After the solution has all flowed into the container, the container can be placed in a refrigerator or freezer at a temperature of 20°C to 0°C, and the solution can be left to stand for 6 to 8 hours before proceeding to the next step.

[0035] In the present invention, a golden algae polysaccharide extraction device and a method for using the same for optimizing polysaccharide extraction efficiency are provided. The crushing device 30 can make the polysaccharides stored in the golden algae cells encounter sediment blocks. The sediment blocks can enter the water inlet chamber 49 through the first water inlet 31 and the shock-absorbing universal base 43 and form a physical collision with the protrusion 50 to break the large or complete sediment blocks into tiny particles, thereby increasing the contact area between the polysaccharide and the solvent, facilitating the penetration of the solvent into the sediment blocks, and improving the dissolution efficiency and polysaccharide yield of the subsequent extraction process. When the sediment blocks are accompanied by the rotating centrifugal force generated by the driving motor 20, they will encounter the arc baffle 36 and be blocked by the arc baffle 36 to enter the arc crushing shell 37. When entering After entering the arc-shaped crushing shell 37, the large crushing balls 38 provided on the inner wall of the arc-shaped crushing shell 37 will produce violent collision, rolling and friction with the sedimentation blocks, and exert a continuous and all-round grinding effect on the golden algae cells and the polysaccharide sedimentation blocks therein, which will help to obtain crushed products with smaller particle size, increase the contact area between polysaccharides and solvents, improve the dissolution efficiency and polysaccharide yield in the subsequent extraction process, and further enable the crushed fine particles to reduce clogging in the solid-liquid separation process such as filtration and centrifugation, improve separation efficiency, and increase the amount of polysaccharides released from cells or sedimentation blocks, thereby improving the polysaccharide yield of the entire extraction process, ensuring the effective use of resources and maximizing economic benefits.

[0036] In the present invention, a golden algae polysaccharide extraction device and a method of using the same for optimizing polysaccharide extraction efficiency are provided with an arc-shaped stirring blade 29 above the secondary cross bar 28. The strong vortex generated during rotation forms a strong shear force and an upward flow, which effectively overcomes the natural sedimentation tendency of the precipitate particles in the solvent and ensures that they are continuously suspended in the stirring area. It can also ensure sufficient mixing and uniform dispersion between the broken precipitate and the solvent, so that each precipitate particle can be surrounded by sufficient solvent and then quickly and fully dissolved, and this also avoids the problem of decreased dissolution rate due to excessive concentration of a certain piece. Secondly, the close cooperation between the arc-shaped stirring blade 29 and the broken crystal discharge port 40 ensures that the newly generated fine precipitate particles are drawn into the efficient stirring environment the moment they leave the crushing device 30, thereby achieving instant and comprehensive contact with the solvent. Moreover, this dissolution effect greatly shortens the process from crushing to dissolution of the precipitate particles, accelerates the dissolution process of the precipitate, and significantly improves the dissolution rate of golden algae polysaccharides. Finally, the arc-shaped stirring blade 29 generates heat during the stirring process, and the heat is evenly distributed through the arc-shaped surface of the arc-shaped stirring blade 29, which is conducive to maintaining the stability of the solution temperature and reducing the influence of thermal effects on the activity of polysaccharides, thereby ensuring the thermodynamic stability of the final solution.

[0037] In the present invention, a golden algae polysaccharide extraction device and a method of using the same for optimizing polysaccharide extraction efficiency adopts a spiral split design for the glass cylinder upper body 11 and the glass cylinder lower body 13, so that each component of the device can be installed, disassembled and replaced by itself, which greatly facilitates the daily maintenance of the equipment; and the transparent glass material used on the surface of the glass cylinder upper body 11 and the glass cylinder lower body 13 allows the operator to intuitively and clearly observe the entire extraction process in the tank, including the crushing of the precipitate block, the stirring state, the solution color, the degree of turbidity and other key information, and golden algae polysaccharides are generally less sensitive to light, and will not cause unnecessary light-induced degradation or denaturation when using a reaction tank made of transparent glass. In addition, the glass material has good chemical inertness and is not easy to react with the solvent or the substance to be extracted, ensuring the purity of the extraction process and the integrity of the extract. Moreover, the surface of the glass material is smooth, without dead corners where dirt and grime can hide, and it is easy to clean, which can effectively prevent cross contamination. The manual drainage assembly 18 fixedly installed at the bottom of the lower body 13 of the glass cylinder can utilize gravity to naturally discharge the extracted polysaccharide solution, and an efficient and smooth discharge process can be achieved without the need for additional power equipment, and the extract can be quickly transferred to subsequent concentration, purification or drying processes, thereby simplifying the process flow and improving production efficiency.

[0038] The above embodiments are only for illustrating the technical concept and features of the present invention, and their purpose is to enable people familiar with the technology in this field to understand the content of the present invention and implement it, and they cannot be used to limit the protection scope of the present invention. Any equivalent changes or modifications made according to the spirit of the present invention should be included in the protection scope of the present invention.

Claims

1. A circular hexagonal support frame, wherein a placement ring is provided at the upper end of the circular hexagonal support frame, a glass cylinder lower body is installed on the surface of the placement ring, a glass cylinder upper body is provided above the glass cylinder lower body, a motor housing is fixedly installed on the upper surface of the glass cylinder upper body with threads, a driving motor is fixedly provided inside the motor housing, a driving shaft is rotatably connected to the output end of the driving motor, a concentration probe is fixedly installed at the lower end of the driving shaft, and a primary cross bar is symmetrically provided on the surface of the lower section of the driving shaft, a crushing device is fixedly installed on the upper surface of the symmetrically provided primary cross bars, a secondary cross bar and a tertiary cross bar are provided above the crushing device, an arc-shaped stirring blade is fixedly connected between the secondary cross bar and the threaded layer, and three vertical stirring rods are arranged in an array on the upper surface of the secondary cross bar, and the vertical stirring rod is located on the inner side of the arc-shaped stirring blade; the crushing device includes a square cross bar. A crushing shell, a water inlet chamber is provided inside the square crushing shell, an arc-shaped crushing shell is provided at the rear end of the square crushing shell, the arc-shaped crushing shell and the square crushing shell are fixedly connected by an arc-shaped baffle, and end blocks are symmetrically provided at both ends of the upper surface of the arc-shaped crushing shell, and the symmetrical end blocks are provided with slide grooves opening inward, an arc-shaped sliding cover is slidably installed in the slide groove, and sliding blocks are symmetrically provided on the lower surfaces of both ends of the arc-shaped sliding cover, the sliding blocks are slidably installed in the slide groove, and a connecting rod is fixedly installed on the outer surface of the arc-shaped sliding cover, a fixing block is fixedly installed on the upper end of the connecting rod, the fixing block is fixedly connected with the formation, and a lower end block is fixedly connected below the square crushing shell, the lower end block is fixed to the primary cross bar, and a second water inlet is provided at the front end opening of the lower end block, and the second water inlet is connected with the water inlet chamber.

2. The golden algae polysaccharide extraction device and the use method thereof for optimizing polysaccharide extraction efficiency according to claim 1, characterized in that: A plurality of large-sized crushing balls are fixedly mounted on the inner wall of the lower surface of the arc-shaped sliding cover and the inner wall of the upper surface of the arc-shaped crushing shell, a crushing groove is provided between the large-sized crushing balls on both sides, the crushing groove is connected with the water inlet chamber, and a crushed crystal discharge outlet is provided on the upper surface of the end of the arc-shaped crushing shell, and a plurality of discharge through holes are provided on the surface of the crushed crystal discharge outlet.

3. The device for extracting golden algae polysaccharides for optimizing polysaccharide extraction efficiency and the method for using the same according to claim 2, characterized in that: A first water inlet is arranged at the front end of the square crushing shell, and vertical through holes are arranged on both sides of the square crushing shell, and the vertical through holes are communicated with the inside of the water inlet cavity.

4. The golden algae polysaccharide extraction device and the use method thereof for optimizing polysaccharide extraction efficiency according to claim 1, characterized in that: The upper surface of the glass cylinder upper body is provided with a plurality of reinforcing lock buckles in an annular array; and the lower surface of the placement ring is provided with a plurality of lower end blocks in an annular array, the lower end blocks are connected with the placement ring in a socket connection and provide secondary reinforcement for the glass cylinder upper body and the glass cylinder lower body.

5. The device for extracting golden algae polysaccharides for optimizing polysaccharide extraction efficiency and the method for using the same according to claim 4, characterized in that: A circular inner arc hole is provided at the bottom of the inner wall of the lower body of the glass cylinder, and a manual liquid discharge component is fixedly installed at the bottom of the lower body of the glass cylinder, and the manual liquid discharge component is communicated with the circular inner arc hole.

6. The device for extracting golden algae polysaccharides for optimizing polysaccharide extraction efficiency and the method for using the same according to claim 5, characterized in that: A stirring chamber is arranged inside the upper body of the glass cylinder, a sedimentation chamber is arranged inside the lower body of the glass cylinder, a threaded layer is arranged on the upper section of the lower body of the glass cylinder, and the stirring chamber is threadedly connected with the threaded layer.

7. The device for extracting golden algae polysaccharides for optimizing polysaccharide extraction efficiency and the method for using the same according to claim 6, characterized in that: An ethanol concentration detection gauge is fixedly mounted on the upper surface of the glass cylinder upper body, the ethanol concentration detection gauge is connected to the concentration probe, and a liquid filling port is arranged on the left side of the ethanol concentration detection gauge, the liquid filling port is connected to the interior of the stirring chamber.

8. The device for extracting polysaccharides from golden algae and the method for using the same for optimizing polysaccharide extraction efficiency according to claim 4, characterized in that: Six support rods are arranged in a circular array on the lower surface of the placement ring, and a shock-absorbing universal base is fixedly installed at the bottom of each support rod.

9. The golden algae polysaccharide extraction device and the use method thereof for optimizing polysaccharide extraction efficiency according to claim 3, characterized in that: A switch button is installed on the upper surface of the motor housing, and the switch button can be divided into a primary button or a secondary button; and a plurality of protrusions are arranged on the inner wall of the water inlet cavity; and a plurality of small crushing balls are arranged inside the arc-shaped baffle.

10. A method for extracting golden algae polysaccharide, characterized in that: The following steps are involved: S1: firstly, heat-treating the golden algae fermentation liquid in a container at 60°C for 10 minutes; after completion, rapidly cooling the solution to room temperature; S2: Then, a Buchner funnel and a bottom container are set up and connected to a vacuum pump to achieve reduced pressure filtration; two medium-speed qualitative filter papers are laid on the bottom of the Buchner funnel, diatomaceous earth with a thickness of about 1-2 cm is evenly laid on the filter paper, and an appropriate amount of pure water is added to fully moisten the filter paper and diatomaceous earth; the heat-treated fermentation liquid is slowly added to the Buchner funnel, and the vacuum pump is started to start reduced pressure filtration. During the filtration process, if the filtration speed is found to slow down, the algae mud blocking the surface of the diatomaceous earth should be promptly removed with a scraper to maintain the filtration speed; when the fermentation liquid has all passed through the filter cake and has been filtered clean, the vacuum pump is turned off and the filtrate is removed; S3: Then, 1% (mass fraction) of activated carbon powder is added to the filtrate, and mechanical stirring is used at room temperature at a speed of 400 revolutions per minute (rpm) for 0.5 hours; this high-speed stirring helps the activated carbon to fully contact with the pigment in the filtrate and effectively adsorb it, thereby achieving a decolorization effect. After the decolorization process is completed, observe the color change of the filtrate; if the filtrate has become clear enough, it means that the decolorization effect is good; if there is still color remaining, it may be necessary to increase the amount of activated carbon or extend the stirring time; S4: Assemble the Buchner funnel and the container, place two medium-speed qualitative filter papers in the Buchner funnel, slowly pour the decolorized golden algae fermentation liquid (containing activated carbon at this time) into the Buchner funnel, turn on the vacuum pump, and use the reduced pressure filtration method to make the liquid pass through the filter paper, so as to leave the activated carbon and other solid impurities on the filter paper; S5: Then connect the Buchner funnel to the container for receiving the filtrate to ensure that the entire system is sealed and the filtration operation can be performed normally; place a 5μm microporous filter membrane in the Buchner funnel; The pore size of this filter membrane is relatively large, and it is mainly used to remove larger particles. Pour the decolorized filtrate into a Buchner funnel equipped with a 5μm filter membrane, and start the vacuum pump for filtration. When the filtration of the 5μm filter membrane is completed, replace it with a 0.22μm microporous filter membrane and continue to filter the remaining decolorized filtrate. The pore size of this filter membrane is smaller, and it can remove smaller particles. During the filtration process, if the filtration speed is found to be significantly slower, it may be because the filter membrane is blocked. At this time, a new filter membrane should be replaced to maintain the filtration efficiency. When all the decolorized filtrate is filtered through the 0.22μm filter membrane, turn off the vacuum pump and collect the filtrate. S6: First pour the filtrate into the lower body of the glass cylinder and then lock the upper body of the glass cylinder upside down. After locking, slowly add 95% ethanol equivalent to four times its volume to the filtrate, and turn on the switch button to start the drive motor to quickly stir the internal solution to ensure that the ethanol and the filtrate are fully mixed; after adding ethanol, the ethanol concentration in the solution should reach 76%, then open the manual drainage component to allow the solution to flow into the container, and after the solution has completely flowed into the container, transfer it to a refrigerator or freezer; let the solution stand at a temperature of -20℃ to 0℃ for 6 to 8 hours; preferably, alcohol precipitation at -20℃, because lower temperatures are conducive to the aggregation and precipitation of polysaccharide molecules; after sufficient alcohol precipitation time, the polysaccharide precipitates from the solution; at this time, the supernatant mainly contains unprecipitated proteins and other soluble substances; S7: pouring away the supernatant in the alcohol-precipitated feed solution, leaving only the sediment at the bottom; Transfer these precipitates to centrifuge tubes and ensure that the weight of the precipitates in all centrifuge tubes is roughly equal to maintain balance during the centrifugation process; unbalanced centrifuge tubes may cause the centrifuge to vibrate, thereby affecting the centrifugation effect. Place the centrifuge tubes containing the precipitates in the centrifuge, set the centrifugation time to 8 minutes, and the centrifugal force to 4000g. The purpose of centrifugation is to further separate the solids and possible residual liquid in the precipitate; after the centrifugation is completed, open the centrifuge tube, pour off the supernatant, and collect the crude polysaccharide wet product left at the bottom of the centrifuge tube; S8: Add an appropriate amount of pure water to the crude polysaccharide wet product obtained by centrifugation to fully dissolve the polysaccharide. The concentration of the solution should be adjusted to 40 g / l. Use medium-speed qualitative filter paper to preliminarily filter the solution to remove larger particles and impurities. The filtered solution is further filtered through a 0.22 μm microporous filter membrane to remove smaller particles and activated carbon that may not be completely removed. During the filtration process, some sticky gray-black colloidal solids may remain on the filter paper. These are mainly activated carbon and proteins denatured by alcohol precipitation. After the filtration is completed, check the clarity of the filtrate. If the filtrate is still not clear enough, further filtration or purification steps may be required. S9: Add four times the volume of 95% ethanol to the filtrate for alcohol precipitation, and repeat step 6; S10: Pour off the supernatant of the precipitate (polysaccharide) after the secondary alcohol precipitation treatment, divide the precipitate into centrifuge tubes and put them into a centrifuge, centrifuge at a centrifugal force of 4000g, usually for 8 minutes, further compress the precipitate and remove the ethanol and water that may remain in the precipitate; after the centrifugation, collect the precipitate, add an appropriate amount of 95% ethanol to mash it, put the mashed precipitate into a vortex oscillator and oscillate for about 1 minute, which helps the ethanol to penetrate the precipitate more deeply, thereby removing more impurities; put the washed and oscillated precipitate into the centrifuge again, and centrifuge it at a centrifugal force of 4000g for 8 minutes; after the centrifugation, pour off the supernatant and retain the washed polysaccharide precipitate; the precipitate obtained at this time is the refined crude polysaccharide wet product; S11: The wet crude polysaccharide product is freeze-dried overnight to obtain a dry refined product; S12: Grind the freeze-dried refined product with a mortar and pass it through an 80-mesh sieve to collect the powder; (Quality inspection: pure water can be used to prepare a 10g / l concentration solution to observe whether it is clear and transparent and whether there are insoluble substances; in this state, the solubility of the product in water is greater than 80g / l and ≤100g / l;).

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