A culture instrument for microbial inoculants

Abstract

The application discloses a kind of microbial inoculant culture apparatus, it is related to microbial inoculant culture technical field, including: incubator, carousel and culture base, the upper portion of the carousel is provided with microbial filtration assembly;Through the setting of quantitative rotating component and culture base lifting assembly, culture base can be fixed in the bottom of push ring, the height difference is smaller when filter membrane falls from fixed tube, will not cause impact when filter membrane and culture base contact due to excessive height difference, to prevent the splash of microorganism on filter membrane in impact, also can prevent filter membrane from producing wrinkle in the process of impact, when it is necessary to add nutrient solution, specified culture base is lifted to the bottom of fixed tube, operator can extend needle cylinder into fixed tube, and nutrient solution is extruded into culture base, so that nutrient solution can be added into culture base without opening incubator, avoid the risk of contamination in culture base when air flow is caused by opening incubator.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of microbial inoculant culture, in particular to a microbial inoculant culture instrument. BACKGROUND

[0002] Before culturing the microbial inoculant, the microorganism needs to be extracted and inoculated into the culture medium, and a limit instrument is needed in the process, and after use, the user transfers the filter membrane into the culture medium.

[0003] In the process of inoculating microorganisms, when the tool is used to transfer the filter membrane, the microorganisms in the surrounding air may settle on the filter membrane or the culture medium, causing contamination, even in a relatively clean environment, such as the air conditioning wind of a general laboratory, the airflow caused by personnel walking, etc., which may carry small particles and microorganisms, increasing the risk of contamination, resulting in failure of the final microbial inoculant culture, or the culture effect is not ideal, after inoculation, the culture medium with the microbial inoculant needs to be added with nutrient solution regularly to meet the growth needs of the microbial inoculant, but when the nutrient solution is added to the culture medium, it is difficult to ensure uniform coverage in the culture medium, so a shaking instrument needs to be used to mix the microbial inoculant and the nutrient solution, and in the process of taking out the culture medium from the culture instrument and shaking, since the culture instrument is opened to drive air flow, it will increase the risk of impurities in the air entering the culture medium and causing contamination, and part of the microorganisms may die due to long-term change of temperature, which will adversely affect the culture effect of the microbial inoculant.

[0004] To solve the above problems, the present application provides a microbial inoculant culture instrument. SUMMARY

[0005] To solve the above technical problems, a microbial inoculant culture instrument is provided.

[0006] To achieve the above purposes, the present application can adopt the following technical solutions:

[0007] The present application provides a microbial inoculant culture instrument, which comprises a culture box, a turntable and a culture base, the turntable is arranged in the culture box, the culture base is fixedly connected to the top of the turntable, and a microbial filtration assembly is arranged above the turntable.

[0008] The microbial filtration assembly comprises a fixed pipe fixedly connected to a culture box, a threaded pipe fixedly connected to the top of the fixed pipe, a fixed frame fixedly connected to the threaded pipe, wherein the fixed frame is not less than two, the two fixed frames are symmetrically arranged on the fixed pipe, a sliding block is slidably connected in the two fixed frames, a spring one is arranged in the two fixed frames, a baffle is fixedly connected to the bottom of the two sliding blocks, a liquid outlet hole is formed in the two baffles, a push block is fixedly connected to the bottom of the two baffles, a fixed rod is fixedly connected to the top of the rotating disc, the fixed rod is symmetrically arranged on both sides of each culture base, and a push ring is fixedly connected to the top of the fixed rod.

[0009] Preferably, a bottle seat is fixedly connected to the top of the rotating disc, and a liquid collecting bottle is arranged in the bottle seat.

[0010] Preferably, the height of the push ring is the same as that of the push block, the two push blocks are V-shaped, and the push ring and the push block form a squeezing fit.

[0011] Preferably, the bottom of the rotating disc is provided with a quantitative rotating assembly, the quantitative rotating assembly comprises a solid cylinder rotatably connected to the bottom of the culture box, a hexagonal rod slidably connected in the solid cylinder, the top of the hexagonal rod is fixedly connected to the rotating disc, a rotating ring is fixedly connected to the side of the solid cylinder, a plurality of fixed teeth are fixedly and annularly connected to the top of the rotating ring, an electric machine is fixedly installed on one side of the inside of the culture box, a disc is sleeved on the output shaft of the electric machine, a rotating rod is rotatably connected to the bottom of the disc, a fixed frame is fixedly installed on the inner bottom of the culture box, a reciprocating block is slidably connected in the fixed frame, the end of the rotating rod away from the disc is rotatably connected to the reciprocating block, and two flaps are fixedly connected to the bottom of the reciprocating block.

[0012] Preferably, the fixed teeth are equidistantly arranged.

[0013] Preferably, the two flaps are arranged in a staggered manner at the bottom of the reciprocating block, an inclined surface is arranged on the side of the two flaps close to the fixed teeth, and the two flaps and the fixed teeth form a pushing fit through the inclined surface.

[0014] Preferably, the bottom of the rotating disc is provided with a culture medium lifting assembly, the culture medium lifting assembly comprises a belt pulley I sleeved on the motor output shaft, a belt pulley II is rotatably connected in the incubator, the belt pulley II is located away from the belt pulley I, a belt is sleeved on the belt pulley I and the belt pulley II, a reciprocating screw rod is coaxially fixedly connected to the top of the belt pulley II, a lifting seat is threadedly connected to the reciprocating screw rod, a push rod is fixedly connected to the side of the lifting seat close to the rotating disc, a pushing groove is formed in the rotating disc, a limiting block is fixedly connected to the side of the lifting seat away from the push rod, a limiting groove is formed in the incubator, the limiting block is slidably connected in the limiting groove.

[0015] Preferably, the pushing grooves are equidistantly arranged, the number of the pushing grooves is the same as that of the rotating disc and the culture medium seat, the shape of the pushing grooves is the same as that of the push rod, and the push rod and the pushing grooves form a sliding fit.

[0016] Preferably, the bottom of the rotating disc is provided with a culture medium oscillation assembly, the culture medium oscillation assembly comprises a mounting rod fixedly connected in the incubator, a wave plate is fixedly connected to the end of the mounting rod away from the incubator, the bottom of the rotating disc is fixedly connected with two connecting rods, the two connecting rods are symmetrically arranged at the bottom of the rotating disc, rollers are arranged at the bottom of the two connecting rods, and the solid cylinder is provided with spring II.

[0017] Preferably, the wave plate is ring-shaped, and the rollers and the wave plate form a rolling fit.

[0018] As described above, the microorganism culture instrument has the following characteristics and advantages:

[0019] Through the quantitative rotating assembly and the culture medium lifting assembly, the culture medium can be lifted upwards until the culture medium contacts the push ring and is fixed at the bottom of the push ring. When the culture medium is at the bottom of the push ring, the height difference of the filter membrane falling from the fixed tube is small, so that the impact of the filter membrane contacting the culture medium is avoided, thereby preventing the microorganisms on the filter membrane from splashing in the impact, and preventing the filter membrane from wrinkling in the impact process. At the same time, the filter membrane with bacteria can be directly transferred into the culture medium under the action of gravity, without the need for the operator to manually transfer the filter membrane into the culture medium with tools, thereby simplifying the microorganism filtration process while ensuring the standardization of the operation of placing the filter membrane into the culture medium. In addition, when it is necessary to add nutrient solution, the specified culture medium can be lifted to the bottom of the fixed tube. At this time, the baffle is opened under the action of the push plate, the operator can stretch the needle cylinder into the fixed tube, and the nutrient solution is squeezed into the culture medium, so that the nutrient solution can be added into the culture medium without opening the incubator, thereby avoiding the risk of contamination caused by air flow when the incubator is opened.

[0020] Through the setting of the quantitative rotating assembly, two misaligned dials successively push the fixed teeth through the inclined surface, so that the fixed teeth drive the rotating ring to rotate under the cooperation of the inclined surface, thereby intermittently and small distance rotating the rotating disc in the process of continuous rotation of the disc, so that the culture medium and the liquid collection bottle can be accurately rotated to the front of the fixed tube, thereby accurately controlling the container at the bottom of the fixed tube, and the automatic switching effect is achieved in the process of using the device, which is convenient to use and ensures the precision of the inoculation operation, and avoids the confusion of multiple culture media and liquid collection bottles when injecting solution, thereby effectively classifying different strains;

[0021] Through the setting of the microbial filtering assembly, when the push ring is accurately and quickly rotated to the front of the fixed tube, the two baffles are completely opened, and the filter membrane without limiting falls into the culture medium at this time, thereby automatically completing the operation of placing the filter membrane into the culture medium, without manual transfer of the filter membrane. The inside of the fixed tube is sterilized using a flame injector in the interval of placing different culture media, which can guide the user to perform the filter operation according to the specification, improve the convenience of use, and avoid the occurrence of non-standard operation, thereby being beneficial to the effect of microbial inoculant culture, automatically falling into the filter membrane, and avoiding the cross-infection problem caused by manual picking up of other strains.

[0022] Through the setting of the wave plate, when the rollers roll upwards on the wave plate, the two rollers lift the rotating disc upwards, so that the rotating disc pulls spring two through the hexagonal rod, and spring two is stretched. When the rollers roll downwards on the wave plate, the rotating disc moves downwards under the action of its own gravity and the tightening of spring two, so that the rotating disc moves up and down during rotation, thereby achieving the effect of oscillation, and then playing the effect of uniformly mixing the nutrient solution and the microorganism after adding the nutrient solution in the culture medium, thereby preventing the situation that the uneven flow of the nutrient solution affects the growth of the microorganism. BRIEF DESCRIPTION OF DRAWINGS

[0023] Figure 1 It is a whole structure perspective view shown in the present application;

[0024] Figure 2 It is a whole structure perspective view shown in the present application;

[0025] Figure 3 It is a whole structure cross-sectional perspective view shown in the present application;

[0026] Figure 4 It is a rotating disc structure perspective view shown in the present application;

[0027] Figure 5 It is a fixed tube structure perspective view shown in the present application;

[0028] Figure 6The fixed tube structure shown in the present application is a bottom view schematic diagram;

[0029] Figure 7 The culture medium lifting assembly structure shown in the present application is a schematic diagram of a perspective view;

[0030] Figure 8 The reciprocating block structure shown in the present application is a bottom view schematic diagram;

[0031] Figure 9 The wave plate structure shown in the present application is a sectional view schematic diagram of a perspective view;

[0032] Figure 10 The wave plate structure shown in the present application is a schematic diagram of a perspective view;

[0033] Figure 11 The solid cylinder internal structure shown in the present application is a sectional view schematic diagram of a perspective view;

[0034] Figure 12 The wave plate structure shown in the present application is a schematic diagram of a perspective view.

[0035] In the present application, the reference numerals are: 1, incubator; 2, turntable; 3, culture base; 4, bottle seat; 5, liquid collection bottle;

[0036] Microbial filtration assembly: 601, fixed tube; 602, threaded tube; 603, fixed frame; 604, sliding block; 605, spring one; 606, baffle; 607, liquid outlet hole; 608, push block; 609, fixed rod; 610, push ring;

[0037] Quantitative rotating assembly: 701, solid cylinder; 702, hexagonal rod; 703, rotating ring; 704, fixed tooth; 705, motor; 706, disc; 707, rotating rod; 708, fixed frame; 709, reciprocating block; 710, tab;

[0038] Culture medium lifting assembly: 801, pulley one; 802, pulley two; 803, reciprocating screw rod; 804, lifting seat; 805, push rod; 806, push groove; 807, limiting block; 808, limiting groove;

[0039] Culture medium oscillation assembly: 901, mounting rod; 902, wave plate; 903, connecting rod; 904, roller; 905, spring two. DETAILED DESCRIPTION

[0040] Clearly, the described embodiments are only a part of the embodiments of the present application, and are not all the embodiments. Based on the embodiments of the present application, all other embodiments obtained by a person of ordinary skill in the art without creative labor fall within the scope of the present application.

[0041] Referring to FIG. 1 to FIG. 4, Figure 12 The culture instrument for microbial inoculants provided by the present application will be described in detail as follows:

[0042] A culture instrument for microbial inoculants, as shown in FIG. 1, comprises a culture box 1, a rotating disc 2 and a culture base 3, the size of the culture base 3 is the same as that of the culture medium, so that the culture medium can be clamped into the culture base 3, the rotating disc 2 is arranged in the culture box 1, the culture base 3 is fixedly connected to the top of the rotating disc 2 at equal intervals, and a microbial filtration assembly is arranged above the rotating disc 2. Figures 1-6

[0043] The microbial filtration assembly comprises a fixed tube 601 fixedly connected to the culture box 1, the fixed tube 601 is located on the movement track of the culture base 3, a threaded tube 602 is fixedly connected to the top of the fixed tube 601, the threaded tube 602 is used for installing a funnel for introducing liquid, a fixed frame 603 is fixedly connected to the threaded tube 602, the number of the fixed frame 603 is not less than two, the two fixed frames 603 are symmetrically arranged on the fixed tube 601, a sliding block 604 is slidingly connected in the two fixed frames 603, a spring 605 is arranged in the two fixed frames 603, the two ends of the spring 605 are fixedly connected with the sliding block 604 and the fixed frame 603 respectively, a baffle 606 is fixedly connected to the bottom of each sliding block 604, the baffles 606 located on the two sliding blocks 604 can form a complete circle, a sealing ring is arranged on the baffle 606 and abuts against the fixed tube 601, a liquid outlet hole 607 is formed in the baffle 606, a push block 608 is fixedly connected to the bottom of each baffle 606, a fixed rod 609 is fixedly connected to the top of the rotating disc 2, the fixed rod 609 is symmetrically arranged on the two sides of each culture base 3, a push ring 610 is fixedly connected to the top of the fixed rod 609, and the push ring 610 is located directly above the culture base 3, a bottle seat 4 is fixedly connected to the top of the rotating disc 2, and the bottle seat 4 is located between every two culture bases 3, the bottle seats 4 are arranged on the rotating disc 2 at equal intervals, a liquid collecting bottle 5 is arranged in the bottle seat 4, the culture base 3 and the bottle seat 4 are arranged on the top of the rotating disc 2 at equal intervals, the height of the push ring 610 is the same as that of the push block 608, the two push blocks 608 are V-shaped, and the push ring 610 and the push block 608 form a squeezing fit.

[0044] Further, as shown in FIG. 5, Figure 4 Figures 7-12 ​​As shown, the bottom of the rotating disc 2 is provided with a quantitative rotating assembly, which comprises a solid cylinder 701 rotatably connected to the bottom of the incubator 1, the solid cylinder 701 is located at the center of the rotating disc 2, a hexagonal rod 702 is slidably connected in the solid cylinder 701, a hole is formed in the upper part of the solid cylinder 701 and matched with the hexagonal rod 702, the top of the hexagonal rod 702 is fixedly connected with the rotating disc 2, a rotating ring 703 is fixedly connected to the side surface of the solid cylinder 701, and the center points of the solid cylinder 701 and the rotating ring 703 coincide, the rotating ring 703 is connected with the solid cylinder 701 through a plurality of round rods, and is located directly below the rotating disc 2, a plurality of fixed teeth 704 are fixedly connected to the top of the rotating ring 703 in a ring shape and at equal intervals, the two ends of the fixed teeth 704 are triangular, a motor 705 is fixedly installed on one side of the inside of the incubator 1, a disc 706 is sleeved on the output shaft of the motor 705, and a ratchet and pawl mechanism one is arranged between the output shaft of the motor 705 and the disc 706. Figure 4 As shown, only when the output shaft of the motor 705 rotates counterclockwise can the disc 706 be driven to rotate, the bottom of the disc 706 is rotatably connected with a rotating rod 707, the rotating rod 707 is eccentrically arranged on the disc 706, a fixing frame 708 is fixedly installed on the inner bottom of the incubator 1, a reciprocating block 709 is slidably connected in the fixing frame 708, a notch is arranged at the connection between the reciprocating block 709 and the fixing frame 708, one end of the rotating rod 707 away from the disc 706 is rotatably connected with the reciprocating block 709, the bottom of the reciprocating block 709 is fixedly connected with two push pieces 710, the two push pieces 710 are arranged at the bottom of the reciprocating block 709 in a staggered manner, and the side of the two push pieces 710 close to the fixed teeth 704 is provided with an inclined surface, the two push pieces 710 form a pushing cooperation with the fixed teeth 704 through the inclined surface.

[0045] Further, as shown in Figure 4 , Figure 7 the bottom of the rotating disc 2 is provided with a culture medium lifting assembly, which comprises a belt pulley one 801 sleeved on the output shaft of the motor 705, and a ratchet and pawl mechanism two is arranged at the connection between the belt pulley one 801 and the output shaft of the motor 705. Figure 4As shown, only when the output shaft of the motor 705 rotates clockwise, the belt pulley one 801 can be driven to rotate, the belt pulley two 802 is rotatably connected in the incubator 1 and is located away from the belt pulley one 801, the belt pulley one 801 and the belt pulley two 802 are sleeved with a belt, the top of the belt pulley two 802 is coaxially fixedly connected with the reciprocating lead screw 803, a ratchet and pawl assembly is arranged at the connection position of the belt pulley two 802 and the reciprocating lead screw 803, the reciprocating lead screw 803 is threadedly connected with the lifting seat 804, the side of the lifting seat 804 close to the turntable 2 is fixedly connected with the push rod 805, the push rod 805 is arranged in the push slot 806, the push slot 806 is arranged at equal intervals, the push slot 806 is located directly below the culture base 3 and the bottle seat 4, the number of the push slot 806 is the same as the total number of the culture base 3 and the bottle seat 4, the shape of the push slot 806 is the same as that of the push rod 805, the push rod 805 and the push slot 806 form a sliding fit, the side of the lifting seat 804 away from the push rod 805 is fixedly connected with the limiting block 807, the limiting groove 808 is arranged in the incubator 1, the limiting groove 808 is located on the side of the incubator 1 close to the limiting block 807, the limiting block 807 is slidably connected in the limiting groove 808, and the limiting block 807 and the limiting groove 808 cooperate to prevent the lifting seat 804 from rotating under the action of the reciprocating lead screw 803, so that the lifting seat 804 vertically ascends and descends.

[0046] Further, as shown in Figure 9 、 Figures 10-12 , the lower portion of the turntable 2 is provided with a culture medium oscillation assembly, the culture medium oscillation assembly comprises a mounting rod 901 fixedly connected in the incubator 1, and the end of the mounting rod 901 away from the incubator 1 is fixedly connected with a wave plate 902, the wave plate 902 is located directly below the turntable 2, the bottom of the turntable 2 is fixedly connected with two connecting rods 903, the two connecting rods 903 are symmetrically arranged at the bottom of the turntable 2, the two connecting rods 903 are provided with rollers 904 at the bottom, the solid cylinder 701 is provided with a spring two 905, and the two ends of the spring two 905 are fixedly connected with the hexagonal rod 702 and the inner bottom of the incubator 1. The wave plate 902 is ring-shaped, and the rollers 904 and the wave plate 902 form a rolling fit.

[0047] In combination with the above-mentioned embodiments, the following is the whole working process and working principle of the above-mentioned embodiments:

[0048] The initial state is:

[0049] The clean culture medium is installed in the culture seat 3, the liquid collecting bottle 5 is installed in the bottle seat 4, the spring 605 is stretched, the slider 604 is located in the fixed frame 603 close to one side of the fixed tube 601, the two baffles 606 are in contact, the bottom of the fixed tube 601 is closed, the push ring 610 is not in contact with the push block 608, the motor 705 is not started, the lifting seat 804 is not lifted, the push rod 805 is located below the turntable 2, the roller 904 is located in the recess of the wave plate 902, and the spring 905 is not expanded.

[0050] The working state is:

[0051] The turntable 2 quantitatively rotates to switch the culture medium and the liquid collecting bottle 5:

[0052] When the container at the bottom of the fixed tube 601 is switched, the motor 705 is started, the output shaft rotates counterclockwise, the motor 705 drives the disc 706 on the output shaft to rotate under the action of the ratchet and pawl mechanism, so that the disc 706 drives the rotating rod 707 to make eccentric motion, at this time the reciprocating block 709 at the other end of the rotating rod 707 is pulled and slides, therefore the disc 706 rotates to drive the reciprocating block 709 to reciprocate in the fixed frame 708 through the rotating rod 707, when the reciprocating block 709 reciprocates, the two staggered push pieces 710 push the fixed teeth 704 through the inclined surface in turn, so that the fixed teeth 704 drive the rotating ring 703 to rotate under the cooperation of the inclined surface, thereby driving the turntable 2 to rotate intermittently and a small distance in the process of continuous rotation of the disc 706, so as to realize the effect of accurately aligning different containers with the bottom of the fixed tube 601, and further realize the effect of automatic switching in the process of using the device, which is convenient to use and ensures the accuracy of the operation of placing the strain.

[0053] The culture medium is lifted to the bottom of the fixed tube:

[0054] In the process of placing the filter membrane into the culture medium, the motor 705 is started, and its output shaft rotates clockwise, thereby driving the pulley 1 801 to rotate under the action of the ratchet and pawl mechanism 2, thereby driving the pulley 2 802 to rotate under the action of the belt, so that the pulley 2 802 drives the reciprocating wire rod 803 to rotate, thereby driving the lifting seat 804 to rise upward, and the lever 805 rises upward through the rotating slot 806 on the rotating disc 2, and then the culture medium is lifted from the bottom of the culture medium under the cooperation of the rotating slot 806, and the culture medium is lifted upward under the drive of the reciprocating wire rod 803, until the culture medium contacts the push ring 610, at this time the motor 705 stops, so that the pulley 2 802 no longer rotates, thereby making the lever 805 abut against the culture medium, and fixing it at the bottom of the push ring 610. When the culture medium is located at the bottom of the push ring 610, the height difference is small when the filter membrane falls from the fixed tube 601, and the impact of the filter membrane contacting the culture medium will not be caused by the too large height difference, thereby preventing the microorganisms on the filter membrane from splashing in the impact, and also preventing the filter membrane from being wrinkled in the impact process. Compared with the existing process of making microbial agent culture medium which needs to use more instruments, a plurality of instruments are not needed, and the operation of manually transferring the filter membrane by using tools is optimized, thereby simplifying the microbial filtration process while ensuring the standardization of the operation of placing the filter membrane into the culture medium.

[0055] Filtering liquid and automatically dropping filter membrane into culture medium:

[0056] When the microorganism to be cultured needs to be added to the culture medium, the filter membrane is placed in the fixed tube 601, and then the liquid collecting bottle 5 is switched to be directly below the fixed tube 601 by using the motor 705, at this time the filter membrane falls onto the two baffles 606 under the action of gravity, then the funnel used for introducing liquid is screwed on the threaded tube 602, and the liquid with microorganisms is poured in, so that the liquid washes the filter membrane and falls into the liquid collecting bottle 5 through the liquid outlet holes 607 on the baffles 606, achieving the effect of collecting waste liquid. After the liquid is poured in, the microorganisms in the liquid are retained on the filter membrane under the action of filtration, and the waste liquid is completely collected into the liquid collecting bottle 5. At this time, the motor 705 is used to rotate the driving disc 2 counterclockwise, so that the push ring 610 at the top of the culture medium first contacts the push block 608, and then the outer arc surface of the push ring 610 cooperates with the inclined surface of the two push blocks 608, so that the push block 608 pushes the baffle 606, so that the baffle 606 drives the sliding block 604 to slide in the fixed frame 603 to the side away from the fixed tube 601 and compresses the spring one 605. When the push ring 610 rotates to the position directly below the fixed tube 601, the two baffles 606 are completely opened, and at this time the filter membrane without limit falls into the culture medium, thereby completing the operation of placing the filter membrane into the culture medium. The inside of the fixed tube 601 is sterilized by the operator using a flame sprayer during the interval of placing different culture media, so as to achieve the effect of guiding the user to perform the filter operation according to the specification, simplify the operation of the operator using tools to place the filter membrane on the limit instrument for filtration, and also avoid the operator using tools to transfer the filter membrane from the limit instrument to the culture medium and place it flat, thereby improving the convenience and avoiding non-standard operation, thereby facilitating the effect of microbial inoculant culture.

[0057] Take out the collected waste liquid:

[0058] During the process of placing multiple filter membranes, the waste liquid of different microorganisms is filtered out by the liquid outlet holes 607 and flows down into different liquid collecting bottles 5 for storage. After the filter membrane is placed, the device can be opened and all liquid collecting bottles 5 containing waste liquid can be taken out at one time, and then unified processing is performed, which is helpful to simplify the culture process of microbial inoculant. Compared with the traditional limit instrument which needs to set multiple one-way valves inside, no one-way valve needs to be set, and the operator does not need to manually rotate the switch, so as to achieve the effect of avoiding waste liquid backflow. Compared with the use of multiple instruments, the use and manufacturing cost of the instrument is reduced, and the situation that the one-way valve in the limit instrument is difficult to clean completely is avoided.

[0059] Provide oscillation mixing for the culture medium after adding nutrient solution:

[0060] In the process of microbial inoculant culture, the nutrient solution needs to be added regularly, the motor 705 is started and counterclockwise rotation is carried out, the culture medium to which the nutrient solution needs to be added is transferred to the directly below of the fixed tube 601, at this time the push ring 610 above the culture medium pushes the two push blocks 608, so that the two baffles 606 are opened, then the motor 705 is started and clockwise rotation is carried out, so that the culture medium is lifted to the bottom of the fixed tube 601 by the lever 805, at this time the needle cylinder containing the nutrient solution is inserted into the fixed tube 601, the nutrient solution can be added to the culture medium, when the nutrient solution is added to the culture medium, the culture medium is reset with the lever 805, then the motor 705 is started and counterclockwise rotation is carried out, so that the rotating disc 2 is rotated, so that the rollers 904 at the bottom of the rotating disc 2 roll along the wave plate 902, when the rollers 904 roll upwards on the wave plate 902, the two rollers 904 lift the rotating disc 2 upwards, so that the rotating disc 2 pulls the spring two 905 through the hexagonal rod 702, so that the spring two 905 is stretched, when the rollers 904 roll downwards on the wave plate 902, the rotating disc 2 moves downwards under the action of its own gravity and the tightening of the spring two 905, so that the rotating disc 2 moves up and down during rotation, so as to achieve the effect of oscillation, so as to mix the nutrient solution and the microorganism evenly after the nutrient solution is added to the culture medium, so as to prevent the situation that the uneven flow of the nutrient solution affects the growth of the microorganism.

[0061] The above only describes the embodiments of the present application, and does not limit the present application, any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A culture device for a microbial inoculant, characterized by, Include: Incubator (1), turntable (2) and culture base (3), the turntable (2) is arranged in the incubator (1), the culture base (3) is fixedly connected on the top of the turntable (2), the top of the turntable (2) is provided with a microbial filtration assembly;The microbial filtration assembly includes a fixed tube (601) fixedly connected to the incubator (1), the top of the fixed tube (601) is fixedly connected with a threaded tube (602), the threaded tube (602) is fixedly connected with a fixed frame (603), the fixed frame (603) is not less than two, two fixed frames (603) are symmetrically arranged on the fixed tube (601), two sliding blocks (604) are slidably connected in two fixed frames (603), two springs (605) are arranged in two fixed frames (603), the bottoms of two sliding blocks (604) are fixedly connected with baffles (606), two baffles (606) are provided with liquid outlet holes (607), the bottoms of two baffles (606) are fixedly connected with push blocks (608), the top of the turntable (2) is fixedly connected with a fixed rod (609), the fixed rod (609) is symmetrically arranged on both sides of each culture base (3), the top of the fixed rod (609) is fixedly connected with a push ring (610); The top of the turntable (2) is fixedly connected with a bottle seat (4), the bottle seat (4) is provided with a liquid collecting bottle (5), the culture base (3) and the bottle seat (4) are equidistantly arranged on the top of the turntable (2); The height of the push ring (610) is the same as that of the push block (608), two push blocks (608) are V-shaped, and the push ring (610) and the push block (608) form a squeezing fit; The bottom of the turntable (2) is provided with a quantitative rotating assembly, the quantitative rotating assembly includes a solid cylinder (701) rotatably connected to the bottom of the incubator (1), a hexagonal rod (702) slidably connected in the solid cylinder (701), the top of the hexagonal rod (702) is fixedly connected with the turntable (2), a rotating ring (703) is fixedly connected to the side of the solid cylinder (701), a plurality of fixed teeth (704) are fixedly and equidistantly connected to the top of the rotating ring (703), a motor (705) is fixedly installed on one side of the inside of the incubator (1), a disc (706) is sleeved on the output shaft of the motor (705), a rotating rod (707) is rotatably connected to the bottom of the disc (706), a fixed frame (708) is fixedly installed on the inner bottom of the incubator (1), a reciprocating block (709) is slidably connected in the fixed frame (708), the end of the rotating rod (707) away from the disc (706) is rotatably connected with the reciprocating block (709), two flaps (710) are fixedly connected to the bottom of the reciprocating block (709). The bottom of the rotating disc (2) is provided with a culture medium lifting assembly, the culture medium lifting assembly comprises a belt pulley I (801) sleeved on the output shaft of the motor (705), a belt pulley II (802) is rotatably connected in the culture box (1), the belt pulley II (802) is located on the side away from the belt pulley I (801), a belt is sleeved on the belt pulley I (801) and the belt pulley II (802), a reciprocating lead screw (803) is coaxially fixedly connected to the top of the belt pulley II (802), a lifting seat (804) is threadedly connected to the reciprocating lead screw (803), a push rod (805) is fixedly connected to the side close to the rotating disc (2) of the lifting seat (804), a rotating groove (806) is formed in the rotating disc (2), a limiting block (807) is fixedly connected to the side away from the push rod (805) of the lifting seat (804), a limiting groove (808) is formed in the culture box (1), the limiting groove (808) is located on the side close to the limiting block (807) in the culture box (1), and the limiting block (807) is slidably connected in the limiting groove (808).

2. The microorganism culture apparatus according to claim 1, wherein The fixed teeth (704) are arranged at equal intervals.

3. The microorganism culture apparatus according to claim 2, wherein Two push pieces (710) are arranged at the bottom of the reciprocating block (709) in a staggered manner, two inclined surfaces are arranged on the side close to the fixed teeth (704) of the two push pieces (710), and the two push pieces (710) are in push cooperation with the fixed teeth (704) through the inclined surfaces.

4. The microorganism culture apparatus according to claim 3, wherein The rotating grooves (806) are arranged at equal intervals, the number of the rotating grooves (806) is the same as that of the rotating disc (2) and the culture medium seat (3), the shape of the rotating grooves (806) is the same as that of the push rod (805), and the push rod (805) is in sliding cooperation with the rotating grooves (806).

5. The microorganism culture apparatus according to claim 1, wherein The bottom of the rotating disc (2) is provided with a culture medium lifting assembly, the culture medium lifting assembly comprises a belt pulley I (801) sleeved on the output shaft of the motor (705), a belt pulley II (802) is rotatably connected in the culture box (1), the belt pulley II (802) is located on the side away from the belt pulley I (801), a belt is sleeved on the belt pulley I (801) and the belt pulley II (802), a reciprocating lead screw (803) is coaxially fixedly connected to the top of the belt pulley II (802), a lifting seat (804) is threadedly connected to the reciprocating lead screw (803), a push rod (805) is fixedly connected to the side close to the rotating disc (2) of the lifting seat (804), a rotating groove (806) is formed in the rotating disc (2), a limiting block (807) is fixedly connected to the side away from the push rod (805) of the lifting seat (804), a limiting groove (808) is formed in the culture box (1), the limiting groove (808) is located on the side close to the limiting block (807) in the culture box (1), and the limiting block (807) is slidably connected in the limiting groove (808).

6. The microorganism culture apparatus according to claim 5, wherein The wave plate (902) is ring-shaped, and the rolling wheel (904) is in rolling cooperation with the wave plate (902).

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