Luminescence engineering system and method based on fungus bioluminescence
Through the fungal bioluminescence engineering system, a fluorescein-rich nutrient solution is made using a caffeic acid circulation reactor and a bacterial fermentation tank, and bioluminescence is achieved through a high-translucent PV tube, which solves the fluorescein toxicity, light source dependence and The problem of insufficient light provides efficient and environmentally friendly lighting solutions suitable for a variety of environments.
Patent Information
- Application Number
- CN202411329741.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-09-24
- Publication Date
- 2024-12-20
AI Technical Summary
In the prior art, plant self-luminescence systems have problems such as fluorescein toxicity, light source dependence, low light output, and limited applicable plant species. The coating technology is easily affected by the environment and is unstable, and the light intensity of the gene editing technology is insufficient.
Using a luminescence engineering system based on fungal bioluminescence, a subsystem of manufacturing a bioluminescent liquid and a transparent luminescent pipe subsystem are used to cultivate self-luminescent bacterial species using a caffeic acid circulation reactor and a bacterial fermentation tank to produce a nutrient solution rich in fluorescein. Bioluminescence is carried out through high-transparent PV tubes to achieve continuous luminescence, and the nutrient solution is recovered and reused through the waste liquid treatment subsystem.
It achieves efficient bioluminescence without the need for continuous addition of materials. It is suitable for street, landscape and building lighting, energy-saving and environmentally friendly, has significantly improved brightness, adapts to various environments, does not poison plants, and has a long lifespan.
Smart Images
Figure CN119161977A_ABST
Abstract
Description
Technical Field
[0001] The present invention relates to the field of bioengineering application technology, and in particular to a luminescence engineering system and method based on fungal bioluminescence. Background Art
[0002] The firefly luciferase sequence was confirmed in 1987; in 2018, a joint research team from the United States and Japan successfully deciphered the genomes of the fireflies Aquatica lateralis and Photinus pyralis.
[0003] In 2003, Professor Cai Detian's team at Hubei University developed a new type of fluorescence promoter specifically for stimulating substances with luminescent properties in plants. Researchers constructed a bioluminescent reporter system by adding luciferin exogenously, but this method has not been widely used because the cost of adding luciferin exogenously is too high and it may be toxic to plants. Many researchers have used genetic engineering methods to introduce bacterial luminescent subsystems into plant plastids to cultivate self-luminous plants that do not require exogenous addition of luciferin, but the light output of such plants is low.
[0004] The luciferase coding gene of fireflies and the fluorescent protein coding gene of jellyfish have been introduced into the plant genome for expression. The introduction of these genes did allow the plants to glow successfully, but the conditions for glowing were relatively harsh and the results were not satisfactory. Plants with the luciferase gene introduced need to receive additional luciferin and adenosine triphosphate (ATP) to emit a relatively weak and short-lived light; plants with jellyfish fluorescent protein need ultraviolet light to glow, and the short-lived fluorescence emitted can only be detected by instruments.
[0005] In 2010, the laboratory of Alexander Krichevsk at the State University of New York in the United States transferred the luminescent gene cluster luxCDABEG from marine bacteria (Photobacterium leiognathi) into the chloroplasts of tobacco, and the resulting transplastomic tobacco was able to emit light without the addition of fluorescein. This is the world's first artificial self-luminous plant. Although the fluorescence emitted by the transgenic luminescent plant obtained in this study is visible to the naked eye, the luminescence intensity is not enough (about 1 million photons per minute), and the luminescence time is not long, which needs further improvement and optimization.
[0006] In 2014, scientists from the US company Bioglow successfully cultivated a self-luminous plant called "Starlight Avatar" through genetic engineering. The researchers introduced the luminescent gene of a bioluminescent bacterium from the ocean into the chloroplast genome of Nicotiana tabacum, enabling it to glow autonomously and continuously without the aid of ultraviolet light. In 2017, scientists from Yunnan Nabo Biotechnology Co., Ltd. introduced the luminescent gene of marine luminescent bacteria into the chloroplasts of tobacco plants and successfully cultivated China's first true night-light plant that can glow continuously.
[0007] The fungal bioluminescence subsystem is hypothesized to consist of at least four components: molecular oxygen, luciferin, NAD(P)H-dependent hydroxylase, and luciferase.
[0008] In 2015, Purtov et al. demonstrated that the chemical component of fluorescein in FBP is 3-hydroxyhistidine.
[0009] In 2017, Kaskova et al. elucidated the structure of fungal oxyluciferin (Kaskova et al, 2017).
[0010] In 2018, Kotlobay et al. identified fungal luciferase and three other key enzymes in FBP, and elucidated the biosynthetic cycle and luminescence mechanism of fungal luciferin. This is the first encodable luminescence subsystem from a eukaryote, and is also considered to be the core metabolic cycle for the construction of current luminescent plants. The key to applying FBP to plants is the raw material caffeic acid. Caffeic acid is a key intermediate in the production of lignin and other key plant metabolites. It is widely present in plants, and the caffeic acid cycle has been successfully expressed in the exogenous eukaryotic organism Pichia pastoris. Relatively simple subsystems, readily available substrates, and existing practices have laid the foundation for the application of the caffeic acid cycle in plants.
[0011] In 2017, Michael S. Strano's team at the Massachusetts Institute of Technology used a nano-bionics method to inject nanoparticles containing firefly luciferase, luciferin, coenzyme A molecules, and semiconductor nanocrystal phosphorus into the leaves of watercress (Nasturtium officinale), and obtained plants that can emit light on their own. The researchers immersed the nanoparticles and the plants in water, and used high pressure to allow the particles to enter the leaves through the stomata. Then, the plant leaves used their own ATP to generate energy, achieving the chemical catalysis of luciferin by luciferase, producing yellow-green light (~560nm). This method is a physical method, which is simpler and more effective than the method of synthetic biology. However, the duration of luminescence after the nanoparticles are introduced using this method is only 3.5 hours, and the brightness is not enough, so it is not a sustainable method.
[0012] In April 2020, scientists from the Institute of Bioorganic Chemistry of the Russian Academy of Sciences and the Department of Biology of Moscow State University used synthetic biology technology to cultivate luminous plants that can emit fluorescence visible to the naked eye. In this study, researchers used tobacco as the research object, in which they used the fungal bioluminescent subsystem to convert caffeic acid (present in all plants) into luciferin. The transformed plants can emit continuous light and are visible to the naked eye. Caffeic acid is an intermediate in the phenylpropanoid pathway in vascular plants. The researchers integrated four genes from the luminous fungus (Neonothopanus nambi) into the tobacco genome through Agrobacterium transformation. They are the luciferase gene nnluz (luciferase), the milk tree alkaloid synthase gene nnhisps (hispidin synthase), the milk tree alkaloid-3-hydroxylase gene nnh3h (hispidin-3-hydroxylase) and the caffeoyl pyruvate hydrolase gene nncph (caffeoyl pyruvate hydrolase). Although scientists have designed short-lived glowing plants in combination with firefly enzymes in the past, the plants combined with fungal DNA this time have a 10-fold increase in brightness and continuous glow without the need for external chemicals; even more surprisingly, these plants produce about 10 billion photons per minute, with a peak wavelength between 500 and 550 nanometers (the green light range in the visible spectrum). What is more important here is that this stable luminescence does not produce toxic substances, nor does it affect the normal growth and development of plants, while bacterial luciferin is expressed in plastids, is not bright enough, and is toxic to plant growth. This feature of the fungal luminescent subsystem will provide a guarantee for the use of synthetic biology to insert this luminescent subsystem into different plants to build self-luminous plants.
[0013] The Russian Academy of Sciences integrated four bioluminescent genes (CPH, HispS, H3H, Luz) from Neonothopanus nambi into the nuclear genome of tobacco plants (Nicotiana tabacum) through Agrobacterium transformation after codon optimization. The bioluminescent subsystem of the fungus can convert caffeic acid (present in all plants) into luciferin through the caffeic acid cycle, allowing the plant to continuously emit light visible to the naked eye.
[0014] In 2023, Du Hao of Zhejiang University led a team to develop luminous plants. They used fungal bioluminescence technology to optimize the endogenous caffeic acid metabolic pathway of plants by introducing catalytic enzyme genes of Brassica napus and Aspergillus nidulans, and combined with the 4 core genes of luminous fungi to successfully create tobacco and morning glory with stronger luminescence.
[0015] Problems in the prior art:
[0016] 1. Disadvantages of using spraying technology: Spraying fluorescent pigments and fluorescent proteins on the surface of plants will block the stomata of plants. It is troublesome to add external light sources, and adding exogenous fluorescent pigments will be toxic to plants. It is easy to be washed away by rain, and the fluorescent pigments are easy to become ineffective.
[0017] 2. Disadvantages of using gene editing transgenic technology: per unit area, the amount of usable luciferin and luciferase produced by the plant itself is relatively small, resulting in a small number of high-energy unstable small molecule luminescent intermediates, causing the brightness of the plant bioluminescence in the entire fungal luminescence pathway to be weak, which cannot meet the normal lighting needs of daily life, and there are not many plant species available. Summary of the invention
[0018] In order to address the deficiencies in the prior art, the present invention provides a luminescent engineering system and method based on fungal bioluminescence. After the system is filled with materials once, there is no need to continuously add materials to produce a nutrient solution containing a large amount of fluorescent substances. The method of bioluminescence by the flow of nutrient solution in a highly transparent PV tube can be applied to projects using bioluminescence for lighting such as street, landscape, and building lighting, which is energy-saving and environmentally friendly.
[0019] In order to achieve the above object, the present invention is implemented through the following technical solutions:
[0020] A luminescent engineering system based on fungal bioluminescence includes a bioluminescent liquid manufacturing subsystem, a transparent luminescent pipeline subsystem, and a liquid supply power control subsystem. The bioluminescent liquid manufacturing subsystem includes a molecular oxygen subsystem and a waste liquid treatment subsystem. The molecular oxygen subsystem is used to introduce oxygen into a nutrient solution containing bioluminescent luciferin and luciferase to manufacture bioluminescent liquid. The input end of the transparent luminescent pipeline subsystem is connected to the molecular oxygen subsystem, and the output end of the transparent luminescent pipeline subsystem is connected to the waste liquid treatment subsystem. The waste liquid treatment subsystem is used to recover the bioluminescent liquid refluxed in the transparent luminescent pipeline subsystem; the liquid supply power control subsystem is used to control the circulation of the bioluminescent liquid in the transparent luminescent pipeline subsystem.
[0021] The bioluminescent liquid manufacturing subsystem also includes a bacterial fermentation tank and a caffeic acid circulation reactor connected to the molecular oxygen subsystem. The bacterial fermentation tank is used to cultivate self-luminous bacteria, and the caffeic acid circulation reactor is connected to the bacterial fermentation tank for manufacturing luciferin and luciferase.
[0022] A feed inlet and an exhaust port are arranged on the top of the bacterial fermentation tank and the coffee acid circulation reactor.
[0023] A storage tank is also arranged between the molecular oxygen subsystem and the transparent luminous pipeline subsystem.
[0024] The waste liquid treatment subsystem comprises a treatment tank, a recovery tank connected to the treatment tank, and a waste liquid tank. The treatment tank is connected to the output end of the transparent luminous pipeline subsystem, and the recovery tank is connected to the caffeic acid circulation reactor.
[0025] The liquid supply power control subsystem includes an electric control box, a water supply pump and a water recovery pump connected to the electric control box. The water supply pump and the water recovery pump are respectively arranged at the input end and the output end of the transparent luminous pipeline subsystem.
[0026] A method based on fungal bioluminescence adopts the above-mentioned luminescence engineering system based on fungal bioluminescence to emit light.
[0027] Compared with the prior art, the present invention has the following beneficial effects:
[0028] 1. After the system of the present invention is filled with materials once, there is no need to continuously add materials to produce a nutrient solution containing a large amount of fluorescent substances. The method of bioluminescence by the flow of the nutrient solution in a highly transparent PV tube can be applied to projects such as street, landscape, and building lighting that use bioluminescence, which is energy-saving and environmentally friendly.
[0029] 2. Compared with the existing technology of spraying fluorescent protein on the surface of plants, the system of the present invention is not affected by the environment, does not poison plants, and has the functions of reusability and long life; compared with the technical circuit of making plants self-luminous through gene editing technology, the system of the present invention provides a unit area of fluorescein quantity and light intensity that far exceeds the self-luminescence of plants. Similarly, the amount of fluorescein contained in the leaf area of a tree is much lower than the amount of fluorescein in a PV tube; compared with the technical circuit of plant self-luminescence, plant self-luminescence is defective, and the number of plant species currently used is only in the single digit. The system method of the present invention can adapt to the bioluminescent lighting of any tree, landscape, street scene and other environments. BRIEF DESCRIPTION OF THE DRAWINGS
[0030] Attached Figure 1 It is a schematic diagram of the process structure of the bioluminescent liquid manufacturing subsystem of the luminescent engineering system based on fungal bioluminescence of the present invention.
[0031] Attached Figure 2 It is a schematic diagram of the flow structure of the liquid supply power control subsystem of the luminescence engineering system based on fungal bioluminescence of the present invention.
[0032] Attached Figure 3 It is a schematic diagram of the flow structure of the transparent luminescent pipeline subsystem of the luminescent engineering system based on fungal bioluminescence of the present invention.
[0033] The numbers shown in the accompanying drawings are: 1. Bioluminescent liquid manufacturing subsystem; 11. Bacteria fermentation tank; 12. Caffeic acid circulation reactor; 13. Molecular oxygen subsystem; 14. Waste liquid treatment subsystem; 141. Treatment tank; 142. Recovery tank; 143. Waste liquid tank; 15. Reserve tank; 2. Transparent luminescent pipeline subsystem; 3. Liquid supply power control subsystem; 31. Electric control box; 32. Water supply pump; 33. Recovery pump; 4. Electric valve. DETAILED DESCRIPTION
[0034] In order to make the purpose, technical solution and advantages of the technical solution of the present invention clearer, the technical solution of the embodiment of the present invention will be clearly and completely described in conjunction with the drawings of specific embodiments of the present invention. The same figure marks in the drawings represent the same parts. It should be noted that the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the described embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.
[0035] Unless otherwise defined, the technical or scientific terms used herein shall have the common meanings understood by persons with ordinary skills in the field to which the present invention belongs. The words "including" and the like used in the present specification and claims mean that the elements or objects appearing before the word include the elements or objects listed after the word and their equivalents, but do not exclude other elements or objects.
[0036] like Figure 1-Figure 3 As shown, the present invention provides a luminescent engineering system based on fungal bioluminescence, including a bioluminescent liquid manufacturing subsystem 1, a transparent luminescent pipeline subsystem 2, and a liquid supply power control subsystem 3. The bioluminescent liquid manufacturing subsystem 1 includes a molecular oxygen subsystem 13 and a waste liquid treatment subsystem 14. The molecular oxygen subsystem 13 is used to introduce oxygen into a nutrient solution containing luciferin and luciferase to produce bioluminescent liquid. The input end of the transparent luminescent pipeline subsystem 2 is connected to the molecular oxygen subsystem 13, and the output end of the transparent luminescent pipeline subsystem 2 is connected to the waste liquid treatment subsystem 14. The waste liquid treatment subsystem 14 is used to recover the bioluminescent liquid refluxed in the transparent luminescent pipeline subsystem 2; the liquid supply power control subsystem 3 is used to control the circulation of the bioluminescent liquid in the transparent luminescent pipeline subsystem 2.
[0037] In this embodiment, a nutrient solution containing luciferin and luciferase can be added to the molecular oxygen subsystem 13, and then oxygen is added through the oxygen inlet of the molecular oxygen subsystem 13 to obtain a bioluminescent liquid; then the liquid supply power control subsystem 3 controls the bioluminescent liquid to circulate in the transparent luminescent pipe subsystem 2, and the transparent luminescent pipe subsystem 2 can be a PV tube, which is wrapped around municipal street lights, landscape lights, landscape trees, buildings and other objects to achieve the luminescent function. After the luminescence process is completed, a certain amount of waste liquid will be generated, which may contain incompletely reacted raw materials, by-products, and biological residues. The waste liquid treatment subsystem 14 collects the waste liquid from the luminescent area to prevent it from polluting the environment.
[0038] Furthermore, the bioluminescent liquid manufacturing subsystem 1 also includes a bacterial fermentation tank 11 and a caffeic acid circulation reactor 12 connected to the molecular oxygen subsystem 13. The bacterial fermentation tank 11 is used to cultivate self-luminous bacteria to ensure the self-sustaining function of the nutrient solution bioluminescence; the concentration of the byproduct pyruvic acid can be adjusted by adjusting the pH value, etc. to accelerate the caffeic acid circulation rate; the caffeic acid circulation reactor 12 is connected to the bacterial fermentation tank 11 and is used to produce luciferin and luciferase.
[0039] Furthermore, a feed port and an exhaust port are provided on the top of the bacterial fermentation tank 11 and the caffeic acid circulation reactor 12, and the exhaust port is used to discharge carbon dioxide.
[0040] In this embodiment, a nutrient solution and required raw materials such as self-luminous bacteria are first added into the bacterial fermentation tank 11 through a feed port. After the self-luminous bacteria are cultured to a certain concentration, the self-luminous bacteria cultured in the bacterial fermentation tank 11 and its metabolites (including caffeic acid, etc.) are introduced into the caffeic acid circulation reactor 12; catalytic enzymes, caffeic acid and other required raw materials are added into the caffeic acid circulation reactor 12 through a feed port. Caffeic acid is first converted into an intermediate product (such as milk tree alkaloids) and then further converted into luciferin through the catalytic action of a series of enzymes in the self-luminous bacteria. At the same time, luciferase is also synthesized and secreted in this process to catalyze the oxidation reaction of luciferin, thereby releasing light energy.
[0041] Furthermore, a storage tank 15 is provided between the molecular oxygen subsystem 13 and the transparent light-emitting pipe subsystem 2 for storing a biological nutrient solution containing luciferin, luciferase, and oxygen.
[0042] Furthermore, the waste liquid treatment subsystem 14 includes a treatment tank 141, a recovery tank 142 connected to the treatment tank 141, and a waste liquid tank 143. The treatment tank 141 is connected to the output end of the transparent luminous pipeline subsystem 2, and the recovery tank 142 is connected to the caffeic acid circulation reactor 12. In this embodiment, the treatment tank 141 is used to recover the nutrient solution refluxed in the transparent luminous pipeline subsystem 2, and can adjust the pH value of the nutrient solution, filter, disinfect and clean it, adjust the concentration of the intermediate product that is too high or too low in the nutrient solution, and then flow the nutrient solution into the recovery tank 142, and then recycle it in the caffeic acid circulation reactor 12; the waste liquid flows into the waste liquid tank 143 for centralized treatment.
[0043] Furthermore, the liquid supply power control subsystem 3 includes an electrical control box 31, a water supply pump 32 and a recovery pump 33 connected to the electrical control box 31, and the water supply pump 32 and the recovery pump 33 are respectively arranged at the input end and the output end of the transparent luminous pipeline subsystem 2.
[0044] The working principle and process of the present invention are as follows:
[0045] A biological nutrient solution containing fungal bioluminescent luciferin (3-hydroxyhistidine) and luciferase Luz is cultivated and manufactured through a bacterial fermentation tank 11 and a caffeic acid circulation reactor 12. At night, molecular oxygen is added to the nutrient solution rich in active luciferin, and then they pass through a durable high-transparency PV tube and flow through various landscape trees, municipal street light poles, and municipal lighting buildings. The fungal luciferin (3-hydroxyhistidine) is oxidized by the fungal luciferase Luz to generate unstable high-energy small molecules, and then the molecular energy decays to release green fluorescence with a wavelength of about 520nm. The fluorescence passes through the high-transparency PV tube to achieve lighting such as fungal bioluminescent street landscapes.
[0046] When the municipal street landscape does not need to be illuminated every morning, the liquid supply power control subsystem 3 of the system of the present invention is used to recycle the biological tissue nutrient solution carrying luciferin through the recovery pump 33. The byproducts after the bioluminescent reaction, such as pyruvic acid and carbon dioxide, are processed. Due to the long-term bioluminescence, the concentration of fungal luciferin (3-hydroxyhistidine) decreases and the concentration of caffeic acid increases. Therefore, it is necessary to use the caffeic acid circulation reactor 12 in the bioluminescent liquid manufacturing subsystem 1 to catalyze the caffeic acid again and regenerate the fungal luciferin (3-hydroxyhistidine) for use in the next day's bio-power generation; in the process of recycling, the waste liquid treatment subsystem 14 in the bioluminescent liquid manufacturing subsystem 1 is used to readjust the pH value and release carbon dioxide.
[0047] After the system of the present invention is added with materials once, there is no need to continuously add materials afterwards. It has its own caffeic acid metabolic circulation device, which is specifically embodied in the use of three parts: a bioluminescent liquid manufacturing subsystem 1, a liquid supply power control subsystem 3, and a transparent luminescent pipeline subsystem 2. The method of artificially manufacturing a nutrient solution containing a large amount of fluorescent substance and causing the nutrient solution to flow in a PV tube for bioluminescence can be applied to projects using bioluminescence for lighting such as street, landscape, and building lighting, which is energy-saving and environmentally friendly.
[0048] Compared with the existing technology of spraying fluorescent protein on the surface of plants, the system of the present invention is not affected by the environment, does not poison plants, and has the functions of reusability and long life; compared with the technical circuit of making plants self-luminous through gene editing technology, the system of the present invention provides a number of fluorescent substances and light intensity per unit area that far exceeds the self-luminescence of plants. Similarly, the amount of fluorescent substances contained in the leaf area of a tree is much lower than the amount of fluorescent substances in a PV tube; compared with the technical circuit of plant self-luminescence, plant self-luminescence is defective, and the number of plant species currently used is only in the single digit, while the system of the present invention can adapt to bioluminescent lighting in any tree, landscape, street scene and other environments.
[0049] The present invention also provides a method based on fungal bioluminescence, which uses the above-mentioned luminescence engineering system based on fungal bioluminescence to emit light under the condition of lamp irradiation.
[0050] Those skilled in the art should understand that the above-mentioned specific embodiments are merely examples and not limitations, and various modifications, combinations, partial combinations and replacements may be made to the embodiments of the present invention according to design requirements and other factors. As long as they are within the scope of the attached claims or their equivalents, they are within the scope of rights to be protected by the present invention.
Claims
1. A luminescent engineering system based on fungal bioluminescence, It is characterized in that It includes a bioluminescent liquid production subsystem, a transparent luminescent pipe subsystem, and a liquid supply power control subsystem. The bioluminescent liquid production subsystem includes a molecular oxygen subsystem and a waste liquid treatment subsystem. The molecular oxygen subsystem is used to introduce oxygen into a nutrient solution containing bioluminescent luciferin and luciferase to produce bioluminescent liquid. The input end of the transparent luminescent pipe subsystem is connected to the molecular oxygen subsystem, and the output end of the transparent luminescent pipe subsystem is connected to the waste liquid treatment subsystem. The waste liquid treatment subsystem is used to recover the bioluminescent liquid refluxed in the transparent luminescent pipe subsystem; the liquid supply power control subsystem is used to control the circulation of the bioluminescent liquid in the transparent luminescent pipe subsystem.
2. A luminescent engineering system based on fungal bioluminescence according to claim 1, It is characterized in that The bioluminescent liquid manufacturing subsystem also includes a bacterial fermentation tank and a caffeic acid circulation reactor connected to the molecular oxygen subsystem. The bacterial fermentation tank is used to cultivate self-luminous bacteria, and the caffeic acid circulation reactor is connected to the bacterial fermentation tank for manufacturing luciferin and luciferase.
3. A luminescent engineering system based on fungal bioluminescence according to claim 2, It is characterized in that A feed inlet and an exhaust port are arranged on the top of the bacterial fermentation tank and the coffee acid circulation reactor.
4. A luminescent engineering system based on fungal bioluminescence according to claim 1, It is characterized in that A storage tank is also arranged between the molecular oxygen subsystem and the transparent luminous pipeline subsystem.
5. A luminescent engineering system based on fungal bioluminescence according to claim 2, It is characterized in that The waste liquid treatment subsystem comprises a treatment tank, a recovery tank connected to the treatment tank, and a waste liquid tank. The treatment tank is connected to the output end of the transparent luminous pipeline subsystem, and the recovery tank is connected to the caffeic acid circulation reactor.
6. A luminescent engineering system based on fungal bioluminescence according to claim 2, It is characterized in that The liquid supply power control subsystem includes an electric control box, a water supply pump and a water recovery pump connected to the electric control box. The water supply pump and the water recovery pump are respectively arranged at the input end and the output end of the transparent luminous pipeline subsystem.
7. A method based on fungal bioluminescence, It is characterized in that A luminescence engineering system based on fungal bioluminescence as described in any one of claims 1 to 6 is used for luminescence.