A skin repair liquid dressing, its preparation method and application

Abstract

The present invention discloses a skin repair liquid dressing, which is prepared by reasonably proportioning carbomer, hyaluronic acid, glycerol, tridecapeptide, allantoin, nano-β-tricalcium phosphate, natural polysaccharide mixture and purified water. The prepared skin repair liquid dressing has a fast film-forming time, has a good repair effect on skin damage, a fast skin healing speed, a high repair efficiency, and has a good antibacterial effect. It can also prevent early pigmentation after wound healing and reduce scars. The natural polysaccharide mixture is composed of pomegranate flower polysaccharide, panax japonicus torr. polysaccharide and polygonatum cyrtonema hua polysaccharide in a reasonable proportion, and can synergistically improve the repair efficiency and inhibit pigment deposition and scar formation. β-tricalcium phosphate has excellent biocompatibility, bioactivity and the ability to promote the formation of new tissues, thereby accelerating the healing of the wound. In addition, the nano-scale particles have a larger specific surface area, which helps to release bioactive ions and further promote the repair process.

Description

Technical Field

[0001] The present invention relates to the technical field of medical dressings, and in particular to a skin repair liquid dressing and a preparation method and application thereof. Background Art

[0002] The skin is the largest organ in the human body. It is in direct contact with the outside world. It prevents the loss of water, electrolytes and nutrients through physical, chemical and biological damage protection mechanisms, becoming the first barrier covering the surface of the human body. The structure of the skin consists of three layers. The outermost layer is the epidermis, followed by the dermis and subcutaneous tissue. Although the structure of the skin has given it a strong ability to resist external invasion, the complex and changeable external environment means that even the skin will still be damaged. Skin damage is very common in daily life, including mild, moderate and severe acne, dermatitis, eczema and other skin problems, as well as skin abrasions, mild burns, sunburn and mild trauma after medical aesthetics. These injuries can cause damage to the skin barrier function, reduce the skin's ability to resist external bacteria, and easily induce inflammatory reactions such as itching, redness, swelling and stinging. In some cases, the injury may also be accompanied by a small amount of bleeding and tissue fluid exudation. Medical dressings play a key role in this process, helping the skin barrier to recover by protecting the wound surface, preventing infection, promoting repair and accelerating healing. When the skin is damaged, the human body loses its barrier. It is crucial to repair the skin quickly and with high quality. Ideally, the skin healing process is a complex and orderly physiological reaction that covers four core stages: hemostasis, inflammatory response, cell proliferation, and tissue remodeling. However, due to various adverse external factors and the patient's own interference, wound repair cannot proceed perfectly according to this process, resulting in the formation of chronic wounds.

[0003] Chronic wounds usually face challenges such as high inflammation, easy infection and irregular wound surface, so it is particularly important to develop targeted medical dressings. Since the "moist healing" theory was proposed, liquid dressings have become the focus of wound dressing research due to their superior water retention and cytoplasmic matrix structure. Natural polymer polysaccharides are widely used in the development of skin wound dressings due to their natural sources, high biocompatibility and low cost. However, most of these dressings are still relatively simple in function and cannot meet the treatment needs of complex chronic wounds. Therefore, the present invention aims to design a liquid dressing specifically for skin repair by combining natural polysaccharides with other functional components, in order to provide new ideas and methods for solving the problem of chronic wounds being difficult to heal. Summary of the invention

[0004] In view of this, the present invention proposes a skin repair liquid dressing and a preparation method and application thereof to solve the above problems.

[0005] The technical solution of the present invention is achieved in this way:

[0006] A skin repair liquid dressing, calculated by weight percentage, comprises the following raw material components: carbomer 0.1% - 1%, hyaluronic acid 0.05% - 0.25%, glycerol 1% - 5%, tridecapeptide 0.05% - 0.1%, allantoin 0.1% - 0.2%, nano-scale β-tricalcium phosphate 0.05% - 0.2%, natural polysaccharide mixture 0.4% - 0.8%, and the balance purified water.

[0007] Preferably, the natural polysaccharide mixture is a mixture of pomegranate flower polysaccharide, panax japonicus torr polysaccharide, and polygonatum cyrtonema hua polysaccharide.

[0008] Preferably, the mass ratio of the pomegranate flower polysaccharide, panax japonicus torr polysaccharide, and polygonatum cyrtonema hua polysaccharide is: (2 - 4) : (1 - 3) : 1.

[0009] Preferably, the preparation method of the pomegranate flower polysaccharide is: after drying the pomegranate flowers, hot water extraction is carried out, filtered, after concentrating the filtrate, ethanol is added to the filtrate, centrifuged after standing, the precipitate is separated out, the precipitate is subjected to deproteinization and decolorization treatment, and after dialysis and freeze-drying, the pomegranate flower polysaccharide is obtained; the hot water extraction conditions include: the temperature is controlled at 80 - 100 °C, the water-to-material ratio is 20 - 30 : 1 (mL / g), and the extraction time is 2 - 3 hours.

[0010] More preferably, in the preparation process of the pomegranate flower polysaccharide, the hot water extraction conditions are: temperature 100 °C, water-to-material ratio 25 : 1 (mL / g), and extraction time 160 min.

[0011] Preferably, the preparation method of the panax japonicus torr polysaccharide is: after drying the panax japonicus torr, enzymatic extraction is carried out with cellulase, filtered, after concentrating the filtrate, ethanol is added to the filtrate, centrifuged after standing, the precipitate is separated out, the precipitate is subjected to deproteinization and decolorization treatment, and after dialysis and freeze-drying, the panax japonicus torr polysaccharide is obtained; the conditions for enzymatic hydrolysis with cellulase include: the enzyme dosage is 0.04 - 0.06 g / g, the enzymatic hydrolysis temperature is 40 - 50 °C, the water-to-material ratio is 30 - 50 : 1 (mL / g), and the enzymatic hydrolysis time is 2 - 3 hours.

[0012] More preferably, in the preparation process of the panax japonicus torr polysaccharide, the cellulase enzymatic hydrolysis conditions are: enzyme dosage 0.05 g / g, enzymatic hydrolysis temperature 45 °C, water-to-material ratio 40 : 1 (mL / g), and extraction time 150 min.

[0013] Preferably, the preparation method of the polygonatum cyrtonema hua polysaccharide is as follows: the dried polygonatum cyrtonema hua is extracted with hot water, filtered, the filtrate is concentrated, ethanol is added to the filtrate, left standing and then centrifuged, the precipitate is separated out, the precipitate is subjected to deproteinization and decolorization treatment, and then dialyzed and freeze-dried to obtain the polygonatum cyrtonema hua polysaccharide; the hot water extraction conditions include: the temperature is controlled at 65-80 °C, the water-to-material ratio is 30-40:1 (mL / g), and the extraction time is 2-3 hours.

[0014] More preferably, in the preparation process of the polygonatum cyrtonema hua, the hot water extraction conditions are: the temperature is 70 °C, the water-to-material ratio is 35:1 (mL / g), and the extraction time is 120 min.

[0015] Preferably, the average particle size of the nano-sized β-tricalcium phosphate is 100-600 nm.

[0016] More preferably, the average particle size of the nano-sized β-tricalcium phosphate is 300 nm.

[0017] The present invention also provides a preparation method of a skin repair liquid dressing, comprising the following steps:

[0018] S1. Take 60% - 80% of the total preparation amount of purified water, slowly add carbomer, hyaluronic acid and glycerol in sequence according to the ratio, stir evenly, leave standing for 1-2 h to fully infiltrate and absorb water and swell, to obtain a premixed solution A;

[0019] S2. Take 20% - 40% of the total preparation amount of purified water, add the thirteen peptide and the natural polysaccharide mixture according to the ratio, and fully stir at a stirring speed of 100-150 r / min at room temperature for 20-30 min to obtain a premixed solution B;

[0020] S3. Slowly pour the premixed solution B into the container containing the premixed solution A, stir evenly, then add allantoin and nano-sized β-tricalcium phosphate, stir evenly, and then a skin repair liquid dressing with uniform texture is obtained.

[0021] The present invention provides the application of the above skin repair liquid dressing in the preparation of skin antibacterial products.

[0022] The present invention provides the application of the above skin repair liquid dressing in the application of preparing products for preventing pigmentation after skin wound healing, reducing scars and promoting wound healing.

[0023] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0024] A skin repair liquid dressing provided by the present invention is prepared by reasonably proportioning carbomer, hyaluronic acid, glycerin, tridecapeptide, allantoin, nano-β-tricalcium phosphate, natural polysaccharide mixture, and purified water. The prepared skin repair liquid dressing has a good repair effect on skin damage, with a fast skin healing speed, high repair efficiency, and good antibacterial effect. It can also prevent early pigmentation after wound healing and reduce scars. The natural polysaccharide mixture is composed of pomegranate flower polysaccharide, panax japonicus torr polysaccharide, and polygonatum cyrtonema hua polysaccharide in a reasonable proportion, and can synergistically improve the repair efficiency, shorten the healing time, and increase the proportion of good repair. Natural polysaccharides have excellent moisturizing and adhesion properties and anti-inflammatory effects, which help to form a protective film, prevent wound surface drying and infection, accelerate wound healing, and inhibit scar formation. β-TCP has excellent biocompatibility, bioactivity, and the ability to promote the formation of new tissues, thus accelerating wound healing. In addition, nano-scale particles have a larger specific surface area, which helps to release bioactive ions and further promote the repair process. In addition, based on the formula of the present invention and using the preparation method of the present invention, the skin repair liquid dressing product has a stable effect, and the active ingredient natural polysaccharide mixture has higher activity. Detailed implementation manners

[0025] To better understand the technical content of the present invention, specific examples are provided below to further illustrate the present invention.

[0026] The experimental methods used in the embodiments of the present invention are all conventional methods unless otherwise specified.

[0027] The materials, reagents, etc. used in the embodiments of the present invention can be obtained from commercial channels unless otherwise specified.

[0028] The preparation method of pomegranate flower polysaccharide is as follows:

[0029] S1. Crush the dried pomegranate flowers, add deionized water to the powder according to a solid-liquid ratio of 25:1 (mL / g), extract at 100 °C for 160 min, filter with gauze, and concentrate the filtrate;

[0030] S2. Add 3 volumes of 90% ethanol to the filtrate, let it stand at 5 °C for 24 h and then centrifuge to separate the precipitate;

[0031] S3. Redissolve the precipitate, add 1 / 5 volume of Sevage solution (the mass ratio of chloroform to n-butanol is 4:1) to remove proteins, shake on a shaker for 30 min, then let it stand and centrifuge to obtain the upper polysaccharide solution, continuously stir in a fume hood for 20 min, let it stand for a while and then layer (if not layered, centrifuge again), take the supernatant and continue to add Sevage to remove impurities, repeat multiple times until there is no protein layer;

[0032] S4. The solution after removing proteins is passed through D101 macroporous adsorption resin, then rotary concentrated, dialyzed (with a molecular weight cut-off of 2000 Da), and freeze-dried for storage to obtain pomegranate flower polysaccharide.

[0033] The preparation method of panax japonicus torr. polysaccharide is as follows:

[0034] S1. After drying, panax japonicus torr. is crushed. Take the panax japonicus torr. powder in a container, add deionized water according to the solid-liquid ratio of 1:40 g / mL, then add cellulase according to the ratio of 0.05 g cellulase / g panax japonicus torr., add a certain amount of citric acid / sodium citrate buffer solution (pH = 5.0), enzymatically hydrolyze at 45 °C for 2.5 h, inactivate at 100 °C for 2 min, filter, and concentrate the filtrate;

[0035] S2. Add 4 volumes of 90% ethanol to the filtrate, let it stand at room temperature for 24 h, then centrifuge to separate the precipitate;

[0036] S3. Redissolve the precipitate, add 1 / 5 volume of Sevage solution (mass ratio of chloroform to n-butanol is 4:1) to remove proteins, shake on a shaker for 30 min, then let it stand and centrifuge to obtain the upper polysaccharide solution. Stir continuously in a fume hood for 20 min, let it stand for a while and then layer (if not layered, perform centrifugation again). Take the supernatant and continue to add Sevage for impurity removal, repeat multiple times until there is no protein layer;

[0037] S4. The solution after removing proteins is passed through D101 macroporous adsorption resin, then rotary concentrated, dialyzed (with a molecular weight cut-off of 1000 Da), and freeze-dried for storage to obtain panax japonicus torr. polysaccharide.

[0038] The preparation method of polygonatum cyrtonema hua polysaccharide is as follows:

[0039] S1. After drying, polygonatum cyrtonema hua is crushed. Take the powder and add deionized water according to the solid-liquid ratio of 1:35, extract at 70 °C for 120 min, filter with gauze, and concentrate the filtrate;

[0040] S2. Add 4 volumes of 90% ethanol to the filtrate, let it stand at 5 °C for 24 h, then centrifuge to separate the precipitate;

[0041] S3. Redissolve the precipitate, add 1 / 5 volume of Sevage solution (mass ratio of chloroform to n-butanol is 4:1) to remove proteins, shake on a shaker for 30 min, then let it stand and centrifuge to obtain the upper polysaccharide solution. Stir continuously in a fume hood for 20 min, let it stand for a while and then layer (if not layered, perform centrifugation again). Take the supernatant and continue to add Sevage for impurity removal, repeat multiple times until there is no protein layer;

[0042] S4. The solution after removing proteins is passed through D101 macroporous adsorption resin, then rotary concentrated, dialyzed (with a molecular weight cut-off of 3000 Da), and freeze-dried for storage to obtain polygonatum cyrtonema polysaccharide.

[0043] Example 1

[0044] A skin repair liquid dressing, by weight percentage, comprises the following raw material components: carbomer 0.1%, hyaluronic acid 0.25%, glycerol 1%, tridecapeptide 0.05%, allantoin 0.2%, nano-scale β-tricalcium phosphate 0.05%, natural polysaccharide mixture 0.4%, and the balance being purified water. The natural polysaccharide mixture is a mixture of pomegranate flower polysaccharide, panax japonicus torr. polysaccharide, and polygonatum cyrtonema polysaccharide; the mass ratio of pomegranate flower polysaccharide, panax japonicus torr. polysaccharide, and polygonatum cyrtonema polysaccharide is 2:1:1; the average particle size of the nano-scale β-tricalcium phosphate is 300 nm.

[0045] The present invention also provides a preparation method of a skin repair liquid dressing, comprising the following steps:

[0046] S1. Take 80% of the total amount of purified water to be prepared, and sequentially and slowly add carbomer, hyaluronic acid, and glycerol in proportion, stir evenly, and let stand for 1 h to fully infiltrate, absorb water, and swell to obtain a premixed solution A.

[0047] S2. Take 20% of the total amount of purified water to be prepared, add tridecapeptide and natural polysaccharide mixture in proportion, and fully stir at a stirring speed of 100 r / min at room temperature for 30 min to obtain a premixed solution B.

[0048] S3. Slowly pour the premixed solution B into the container containing the premixed solution A, stir evenly, then add allantoin and hydroxyapatite, and stir evenly to obtain a skin repair liquid dressing with uniform texture.

[0049] Example 2

[0050] A skin repair liquid dressing, by weight percentage, comprises the following raw material components: carbomer 0.5%, hyaluronic acid 0.2%, glycerol 3%, tridecapeptide 0.08%, allantoin 0.15%, nano-scale β-tricalcium phosphate 0.15%, natural polysaccharide mixture 0.6%, and the balance being purified water. The natural polysaccharide mixture is a mixture of pomegranate flower polysaccharide, panax japonicus torr. polysaccharide, and polygonatum cyrtonema polysaccharide; the mass ratio of pomegranate flower polysaccharide, panax japonicus torr. polysaccharide, and polygonatum cyrtonema polysaccharide is 3:2:1; the average particle size of the nano-scale β-tricalcium phosphate is 300 nm.

[0051] The present invention also provides a preparation method of a skin repair liquid dressing, comprising the following steps:

[0052] S1. Take 60% of the total preparation amount of purified water, and slowly add carbomer, hyaluronic acid, and glycerin in sequence according to the proportion, stir evenly, and let it stand for 2 h to fully soak and absorb water and swell, obtaining premixed solution A;

[0053] S2. Take 40% of the total preparation amount of purified water, add the thirteen - peptide and the natural polysaccharide mixture according to the proportion, and stir fully at a stirring speed of 120 r / min at room temperature for 30 min to obtain premixed solution B;

[0054] S3. Slowly pour premixed solution B into the container containing premixed solution A, stir evenly, then add allantoin and hydroxyapatite, and stir evenly to obtain a skin - repairing liquid dressing with uniform texture.

[0055] Example 3

[0056] A skin - repairing liquid dressing, calculated by weight percentage, includes the following raw material components: carbomer 1%, hyaluronic acid 0.05%, glycerin 5%, thirteen - peptide 0.1%, allantoin 0.1%, nano - scale β - tricalcium phosphate 0.2%, natural polysaccharide mixture 0.8%, and the balance is purified water. The natural polysaccharide mixture is a mixture of pomegranate flower polysaccharide, panax japonicus torr. polysaccharide, and polygonatum cyrtonema hua polysaccharide; the mass ratio of pomegranate flower polysaccharide, panax japonicus torr. polysaccharide, and polygonatum cyrtonema hua polysaccharide is 4:3:1; the average particle size of nano - scale β - tricalcium phosphate is 300 nm.

[0057] The present invention also provides a preparation method of a skin - repairing liquid dressing, including the following steps:

[0058] S1. Take 80% of the total preparation amount of purified water, and slowly add carbomer, hyaluronic acid, and glycerin in sequence according to the proportion, stir evenly, and let it stand for 1 h to fully soak and absorb water and swell, obtaining premixed solution A;

[0059] S2. Take 20% of the total preparation amount of purified water, add the thirteen - peptide and the natural polysaccharide mixture according to the proportion, and stir fully at a stirring speed of 150 r / min at room temperature for 20 min to obtain premixed solution B;

[0060] S3. Slowly pour premixed solution B into the container containing premixed solution A, stir evenly, then add allantoin and hydroxyapatite, and stir evenly to obtain a skin - repairing liquid dressing with uniform texture.

[0061] Comparative Example 1

[0062] The difference between this comparative example and Example 2 is that the skin - repairing liquid dressing in this example does not contain nano - scale β - tricalcium phosphate; the preparation method of the skin - repairing liquid dressing in this example is basically the same as that in Example 2.

[0063] Comparative Example 2

[0064] The difference between this comparative example and Example 2 is that the skin repair liquid dressing in this example does not contain the natural polysaccharide mixture; the preparation method of the skin repair liquid dressing in this example is basically the same as that in Example 2.

[0065] Comparative Example 3

[0066] The difference between this comparative example and Example 2 is that the natural polysaccharide mixture in the skin repair liquid dressing in this example does not contain pomegranate flower polysaccharide, and the natural polysaccharide mixture is a mixture of panax japonicus torr. polysaccharide and polygonatum cyrtonema hua polysaccharide; the mass ratio of panax japonicus torr. polysaccharide to polygonatum cyrtonema hua polysaccharide is: 2:1; the preparation method of the skin repair liquid dressing in this example is basically the same as that in Example 2.

[0067] Comparative Example 4

[0068] The difference between this comparative example and Example 2 is that the natural polysaccharide mixture in the skin repair liquid dressing in this example does not contain panax japonicus torr. polysaccharide, and the natural polysaccharide mixture is a mixture of pomegranate flower polysaccharide and polygonatum cyrtonema hua polysaccharide; the mass ratio of pomegranate flower polysaccharide to polygonatum cyrtonema hua polysaccharide is: 3:1; the preparation method of the skin repair liquid dressing in this example is basically the same as that in Example 2.

[0069] Comparative Example 5

[0070] The difference between this comparative example and Example 2 is that in the natural polysaccharide mixture in the skin repair liquid dressing in this example, polygonatum cyrtonema hua polysaccharide is replaced by pueraria lobata polysaccharide, and the natural polysaccharide mixture is a mixture of pomegranate flower polysaccharide, panax japonicus torr. polysaccharide, and pueraria lobata polysaccharide; the mass ratio of pomegranate flower polysaccharide, panax japonicus torr. polysaccharide, and pueraria lobata polysaccharide is: 3:2:1; the preparation methods of pueraria lobata polysaccharide and polygonatum cyrtonema hua polysaccharide are the same.

[0071] Comparative Example 6

[0072] The difference between this comparative example and Example 2 is that the average particle size of the nano-level β-tricalcium phosphate in the skin repair liquid dressing in this example is 30 μm; the preparation method of the skin repair liquid dressing in this example is basically the same as that in Example 2.

[0073] Comparative Example 7

[0074] The difference between this comparative example and Example 2 is that in the preparation process of the polygonatum cyrtonema hua polysaccharide in the skin repair liquid dressing in this example, the hot water extraction conditions are the same as those in the preparation process of pomegranate flower polysaccharide, which are: temperature 100 °C, water-to-material ratio 25:1 (mL / g), and extraction time 160 min.

[0075] Test Example 1 Safety Experiment

[0076] The skin repair liquid dressings of Examples 1-3 and Comparative Examples 1-7 were respectively subjected to animal skin irritation tests, skin sensitization tests, and in vitro cytotoxicity tests, and the results showed that they were safe and non-toxic.

[0077] Test Example 2 Bacteriostatic Experiment

[0078] According to the "Examination Method for Bacteriostatic Efficacy" in the Chinese Pharmacopoeia 2020 Edition, the bacteriostatic efficacy was determined by measuring the number of colonies surviving in the Sabouraud's dextrose broth of the test solution after 24 hours. The test bacteria were: Escherichia coli, Pseudomonas aeruginosa, Pseudomonas aeruginosa, Candida albicans, Aspergillus niger, Propionibacterium acnes. The bacteriostatic rates of Examples 1-3 and Comparative Examples 1-7 are shown in Table 1 below.

[0079] Table 1 Bacteriostatic Effects of Skin Repair Liquid Dressings

[0080]

[0081] As can be seen from the above table, the skin repair liquid dressings prepared by the present invention have an inhibition rate of more than 99.9% against Escherichia coli, Pseudomonas aeruginosa, Pseudomonas aeruginosa, Candida albicans, Aspergillus niger, and Propionibacterium acnes; in Comparative Example 1, nano-β-tricalcium phosphate was not added, and its bacteriostatic effect was slightly weakened, indicating that the addition of nano-β-tricalcium phosphate has a good barrier effect and can more effectively isolate bacteria, providing a better shielding environment for the wound repair of skin tissues; in Comparative Example 2, the natural polysaccharide mixture was not added, and its bacteriostatic effect was significantly weakened. The results of Comparative Examples 3-5 and Comparative Example 7 showed that pomegranate flower polysaccharide, Panax japonicus Torr. polysaccharide, and Polygonatum cyrtonema Hua polysaccharide have a synergistic effect, and the extraction method of polysaccharides also has a certain influence on their bacteriostatic activity effect.

[0082] Test Example 3 Wound Repair Performance

[0083] 110 SPF-grade SD rats were taken; they were randomly divided into 11 groups with 10 rats in each group, and were respectively smeared with the skin repair liquid dressings prepared in Examples 1-3, Comparative Examples 1-7, and a commercially available recombinant collagen skin repair dressing (brand Kefumei). Each rat was anesthetized by intraperitoneal injection of 1.5 ml / kg of 10% chloral hydrate, and then the back hair was removed and the skin was disinfected. A skin area of 1.5 cm was excised on the back of each rat. 2Full-thickness wounds. Then, the skin repair liquid dressings of Examples 1-3, Comparative Examples 1-7, and a commercially available recombinant collagen skin repair dressing were respectively applied to the wound surfaces, once in the morning and once in the evening every day. The healing of the rat wounds and the scar conditions after complete healing were observed on the 2nd, 4th, and 6th days after spraying the medicine. Wound healing rate = (initial wound area - wound area measured on the nth day) / initial wound area × 100%; after complete healing, the wound surface was closed, no scar was seen, and the skin surface was flat, which was judged as good repair, and the proportion of the number of good repairs was counted; the experimental results are shown in Table 2.

[0084] Table 2 Wound repair performance of skin repair liquid dressings

[0085]

[0086] The above results show that the skin repair liquid dressings prepared in Examples 1-3 of the present invention have a fast film-forming time, have a good repair effect on skin damage, have a fast skin healing rate, and a high repair efficiency. In Comparative Example 1, nano-scale β-tricalcium phosphate (β-TCP) was not added, and the wound healing rate was significantly slowed down. In Comparative Example 6, the repair effect was also weakened to a certain extent after adding micro-scale β-tricalcium phosphate, indicating that β-TCP can significantly promote the repair of skin wounds. β-TCP has excellent biocompatibility, bioactivity, and the ability to promote the formation of new tissues, stimulates cell proliferation and migration, thereby accelerating the healing of the wound surface. In addition, nano-scale particles have a larger specific surface area, which helps to release bioactive ions and further promote the repair process. β-TCP can also regulate the wound microenvironment, reduce the inflammatory response, reduce the degree of fibrosis, and thus reduce scar formation. These mechanisms work together to contribute to better healing and reduce scar formation. Compared with Comparative Examples 2-5 and Comparative Example 7, adding a certain ratio of the self-made pomegranate flower polysaccharide, panax japonicus torr polysaccharide, and polygonatum cyrtonema hua polysaccharide of the present invention can significantly improve the repair efficiency, shorten the healing time, and increase the proportion of good repairs through synergistic effects. Natural polysaccharides have excellent moisturizing and adhesion properties, which help to form a protective film to prevent the wound surface from drying and being infected. Polysaccharides can also stimulate cell proliferation and migration, accelerate the healing of the wound surface. In addition, natural polysaccharides usually have anti-inflammatory effects and can reduce the inflammatory response, thereby reducing scar formation.

[0087] The above are only the preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A skin repair liquid dressing, characterized in that: The composition is composed of the following raw material components by weight percentage: 0.1% to 1% of carbomer, 0.05% to 0.25% of hyaluronic acid, 1% to 5% of glycerol, 0.05% to 0.1% of tridecapeptide, 0.1% to 0.2% of allantoin, 0.05% to 0.2% of nano-scale β-tricalcium phosphate, 0.4% to 0.8% of natural polysaccharide mixture, and the balance of purified water; The natural polysaccharide mixture is a mixture of pomegranate flower polysaccharide, Panax notoginseng polysaccharide and Polygonatum cyrtonema polysaccharide; the mass ratio of the pomegranate flower polysaccharide, Panax notoginseng polysaccharide and Polygonatum cyrtonema polysaccharide is: (2-4): (1-3):

1.

2. A skin repair liquid dressing according to claim 1, characterized in that: The preparation method of the pomegranate flower polysaccharide comprises the following steps: extracting the dried pomegranate flowers with hot water, filtering, concentrating the filtrate, adding ethanol to the filtrate, standing the filtrate and centrifuging to separate the precipitate, deproteinizing and depigmenting the precipitate, dialyzing and freeze-drying to obtain the pomegranate flower polysaccharide; the hot water extraction conditions include: controlling the temperature to 80-100°C, the water-to-material ratio to 20-30:1 (mL / g), and the extraction time to 2-3 hours.

3. The skin repair liquid dressing according to claim 1, characterized in that: The preparation method of the polysaccharide of Panax japonicus is as follows: extracting the dried Panax japonicus by enzymatic hydrolysis with cellulase, filtering, concentrating the filtrate, adding ethanol to the filtrate, standing it and then centrifuging it to separate the precipitate, deproteinizing and depigmenting the precipitate, dialyzing and freeze-drying it to obtain the polysaccharide of Panax japonicus; The conditions for enzymolysis of the cellulase include: enzyme dosage of 0.04-0.06 g / g, enzymolysis temperature of 40-50° C., water-to-material ratio of 30-50:1 (mL / g), and enzymolysis time of 2-3 hours.

4. The skin repair liquid dressing according to claim 1, characterized in that: The preparation method of the polygonatum cyrtonema polysaccharide is as follows: extracting the dried polygonatum cyrtonema with hot water, filtering, concentrating the filtrate, adding ethanol to the filtrate, standing and centrifuging to separate the precipitate, deproteinizing and depigmenting the precipitate, dialyzing and freeze-drying to obtain the polygonatum cyrtonema polysaccharide; the hot water extraction conditions include: controlling the temperature to 65-80°C, the water-to-material ratio to 30-40:1 (mL / g), and the extraction time to 2-3 hours.

5. The skin repair liquid dressing according to claim 1, characterized in that: The average particle size of the nano-scale β-tricalcium phosphate is 100-600 nm.

6. The method for preparing a skin repair liquid dressing according to any one of claims 1 to 5, characterized in that: The following steps are involved: S1. Take 60% to 80% of the total amount of purified water, slowly add carbomer, hyaluronic acid and glycerin in proportion, stir evenly, and let it stand for 1 to 2 hours to allow it to fully absorb water and swell to obtain premix A; S2. Take 20% to 40% of the total amount of purified water, add tridecapeptide and natural polysaccharide mixture in proportion, stir at room temperature at a stirring speed of 100 to 150 r / min for 20 to 30 minutes to obtain premix B; S3. Slowly pour premix B into the container containing premix A, stir evenly, then add allantoin and nano-scale β-tricalcium phosphate, stir evenly, and obtain a skin repair liquid dressing with uniform texture.

7. Use of the skin repair liquid dressing according to any one of claims 1 to 5 in the preparation of skin antibacterial products.

8. Use of a skin repair liquid dressing according to any one of claims 1 to 5 in the preparation of products for preventing pigmentation after skin wound healing, reducing scars and promoting wound healing.

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