White gourd waste microbial organic fertilizer and preparation method thereof

Abstract

The invention provides a wax gourd waste microbial organic fertilizer and a preparation method thereof. The wax gourd waste microbial organic fertilizer comprises 20-50 parts of a microbial flora combined enzyme yeast agent and 100-200 parts of a wax gourd waste organic fertilizer. White gourd wastes and waste chicken feathers are used as raw materials, so that white gourds are rich in citrulline and protein, the chicken feathers contain about 91% of keratin, 9.64% of nitrogen, 0.11% of phosphorus and 0.15% of potassium, and the fertilizer is an amino acid comprehensive fertilizer mainly containing nitrogen. The sugar mud contains various elements such as protein, sugar, calcium, iron, zinc and magnesium, the nutrient substances are fully kept and utilized through enzyme enzymolysis, nutrient absorption and growth of plants are promoted, and various microorganisms in the sugar mud are used for adjusting soil flora, improving the soil structure and improving the soil fertility; the saline-alkali content of the saline-alkali soil is improved, neutralized and corrected to reach the environment required by crop growth.

Description

Technical Field

[0001] The invention belongs to the technical foundation field of wax gourd waste utilization, and specifically relates to a wax gourd waste microbial organic fertilizer and a preparation method thereof. Background Art

[0002] Winter melon is an annual vine herbaceous plant of the genus Winter melon in the Cucurbitaceae family. Winter melon pulp is rich in citrulline and protein. Winter melon waste can be comprehensively utilized through certain technical means, turning waste into treasure, increasing the added value of winter melon, increasing the economic income of melon farmers, reducing pollution to water resources and the environment, and creating greater social and economic benefits.

[0003] Chicken feathers are a kind of pure keratin, with crude protein of about 91%, rich in amino acids. Amino acids are organic compounds and the basic units of protein synthesis. Amino acids can provide organic carbon sources and can be directly absorbed by crops. They can improve the quality of crops, enhance the resistance of crops to salt pits, and improve the ecological environment. Therefore, amino acid fertilizers have great development prospects. Chicken feathers contain 9.64% nitrogen, 0.11% phosphorus, and 0.15% potassium. They are a comprehensive fertilizer source mainly containing nitrogen. Amino acids are produced by enzymatic hydrolysis of chicken feathers with homemade enzymes and waste winter melons. They are developed into microbial organic fertilizers and spread in rice fields when rice is heading to increase seed weight and improve rice quality. The yield of fertilized rice fields is significantly different from that of the control.

[0004] The main components of sugarcane mud are broken fiber, fat, protein, minerals and a small amount of sugar. It also contains nitrogen, phosphorus, potassium and other nutrients that can be absorbed by crops. After activation treatment, the organic matter in it is further reorganized and humified, and it becomes a new type of efficient, safe and high-quality bio-fertilizer that meets the needs of organic and green agricultural production. The application of these technologies saves a lot of water and energy for sugarcane sugar companies, reduces waste emissions and damage to the environment, promotes the sustainable development of the sugar industry, reduces the cost of waste residue treatment, and realizes the resource utilization of sludge.

[0005] Durian shells are rich in carbohydrates, proteins, fats, vitamins, dietary fiber and other nutrients. Tofu hawthorn is rich in protein, cellulose, potassium, magnesium and iron, with 7-8 grams of protein in every 100 grams of bean dregs. The liquid produced by enzymatic decomposition, conversion and catalytic fermentation under natural conditions is similar to natural vinegar biofertilizer, containing natural proteins, minerals and enzymes in the raw materials. Enzymes are catalytic biopolymers that can speed up reactions without changing the direction of the reaction and the product of the biocatalyst. The use of winter melon waste to develop microbial organic fertilizer has a simple manufacturing process, easy-to-obtain raw materials, low cost and wide application. The production process of developing microbial organic fertilizer using winter melon waste is environmentally friendly, and the (liquid, solid) fertilizer produced by decomposition, conversion and catalytic fermentation under such natural conditions forms a complex and stable ecological complex with multiple functions. Summary of the invention

[0006] In view of this, the main purpose of the present invention is to provide a wax gourd waste microbial organic fertilizer and a preparation method thereof.

[0007] To achieve the above object, the technical solution of the present invention is achieved as follows:

[0008] The embodiment of the present invention provides a wax gourd waste microbial organic fertilizer, comprising 20-50 parts of a microbial flora combination enzyme yeast agent and 100-200 parts of wax gourd waste organic fertilizer.

[0009] In the above scheme, the winter melon waste organic fertilizer includes 100 parts of winter melon vines, 100 parts of waste chicken feathers, 20-30 parts of durian shells, 20-30 parts of tofu haws, 20-25 parts of sugarcane mud, and 2-5 parts of acetic acid.

[0010] In the above scheme, the winter melon waste organic fertilizer includes 200 parts of waste winter melon crushed juice and concentrated juice and 20 to 25 parts of waste molasses liquid.

[0011] In the above scheme, the microbial flora combined enzyme yeast agent includes 1-10 parts of thermophilic amylase Streptomyces, 1-10 parts of thermophilic cellulose degrading bacteria, 1-10 parts of thermophilic stearothermophilic Bacillus, 1-10 parts of protein enzyme, and 1-10 parts of Bacillus laterosporus.

[0012] In the above scheme, the waste wax gourd crushed juice concentrate is the crushed juice concentrate of waste wax gourd, rind, seeds and / or pulp.

[0013] The embodiment of the present invention also provides a method for preparing wax gourd waste microbial organic fertilizer, the method comprising:

[0014] Obtain bacterial liquid, preserve the strains according to a 1:1 ratio, culture the strains according to a 1:1 ratio, expand the original bacterial population according to a 1:1 ratio, ferment the liquid according to a 1:1 ratio, and dry the preparation to prepare a microbial flora combined enzyme yeast agent;

[0015] The prepared wax gourd waste organic fertilizer and microbial flora combined enzyme yeast are pre-treated by shearing and stirring in a 3t reactor, mixed evenly, and then fermented. After enzymatic hydrolysis and decomposition, they are filtered, separated into dry and wet parts, and dried to obtain wax gourd waste microbial organic fertilizer.

[0016] In the above scheme, the prepared wax gourd waste organic fertilizer specifically comprises: putting 100 parts of wax gourd vines, 100 parts of waste chicken feathers, 20-30 parts of durian shells, 20-30 parts of tofu haws, 20-25 parts of sugarcane mud, and 2-5 parts of acetic acid into a 3t reaction tank, shearing, crushing, and stirring, and after fermenting for 30-60 days, taking out the decomposed waste chicken feathers and wax gourd vine powder, stirring evenly, and naturally drying them in the sun until the water content reaches 30%, so as to obtain solid wax gourd waste organic fertilizer.

[0017] Compared with the prior art, the present invention uses winter melon waste and waste chicken feathers as raw materials, so that the winter melon is rich in citrulline and protein, and the chicken feathers contain about 91% keratin, 9.64% nitrogen, 0.11% phosphorus, and 0.15% potassium, which is a nitrogen-based amino acid comprehensive fertilizer. Sugar mud contains protein, sugar, calcium, iron, zinc, magnesium and other elements. Through enzyme hydrolysis, the above nutrients can be fully maintained and utilized, promoting plant nutrient absorption and growth, using a variety of microorganisms in sugar mud to regulate soil flora, improve soil structure, increase soil fertility, improve, neutralize and correct the salinity and alkali content of saline-alkali land to achieve the environment required for crop growth. DETAILED DESCRIPTION

[0018] In order to make the purpose, technical solution and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0019] The embodiment of the present invention provides a wax gourd waste microbial organic fertilizer, comprising 20-50 parts of a microbial flora combination enzyme yeast agent and 100-200 parts of wax gourd waste organic fertilizer.

[0020] In some embodiments, the winter melon waste organic fertilizer includes 100 parts of winter melon vines, 100 parts of waste chicken feathers, 20-30 parts of durian shells, 20-30 parts of tofu haws, 20-25 parts of sugarcane mud, and 2-5 parts of acetic acid.

[0021] In some embodiments, the winter melon waste organic fertilizer includes 200 parts of waste winter melon crushed juice and concentrated juice and 20-25 parts of waste molasses liquid.

[0022] In some embodiments, the microbial flora combined enzyme yeast agent includes 1-10 parts of thermophilic amylase Streptomyces, 1-10 parts of thermophilic cellulose degrading bacteria, 1-10 parts of thermophilic Bacillus stearothermophilus, 1-10 parts of protein enzyme, and 1-10 parts of Bacillus laterosporus.

[0023] In some embodiments, the waste wax gourd crushed juice concentrate is the crushed juice concentrate of waste wax gourd, rind, seeds and / or pulp.

[0024] The embodiment of the present invention also provides a method for preparing wax gourd waste microbial organic fertilizer, the method comprising:

[0025] Step 101: obtaining bacterial liquid, preserving the strains at a ratio of 1:1, culturing the strains at a ratio of 1:1, expanding the original bacterial population at a ratio of 1:1, liquid fermenting the bacterial population at a ratio of 1:1, and drying the preparation to prepare a microbial flora combined enzyme yeast agent;

[0026] Step 102: The prepared wax gourd waste organic fertilizer is pre-treated by shearing and stirring in a 3t reactor and then mixed evenly, and then a microbial flora combination enzyme yeast agent is added for fermentation. After enzymatic hydrolysis and composting, it is filtered, separated into dry and wet parts, and dried to obtain the wax gourd waste microbial organic fertilizer.

[0027] Specifically, 100 parts of wax gourd vines, 100 parts of waste chicken feathers, 20-30 parts of durian shells, 20-30 parts of tofu haws, 20-25 parts of sugarcane mud and 2-5 parts of acetic acid are put into a 3t reaction tank for shearing, crushing and stirring. After fermentation for 30-60 days, the decomposed waste chicken feathers and wax gourd vine powder are taken out and stirred evenly, and then naturally dried in the sun until the water content reaches 30%, so as to obtain solid wax gourd waste organic fertilizer.

[0028] Furthermore, 100 parts of solid wax gourd waste organic fertilizer, 1 part of water retaining agent, 1 part of polyacrylamide (salt-tolerant), 1-2 parts of gibberellin 0.5 mg / L, 2-4 parts of potassium dihydrogen phosphate, and 1 part of carbendazim are stirred to prepare a salt-resistant and disease-proof seedling medium.

[0029] 200 parts of waste wax gourd crushed juice concentrate, 20-25 parts of waste molasses liquid, and 50 parts of microbial flora combined enzyme yeast agent are put into a 3t reactor for shearing, stirring, fermentation, composting, quality inspection, and drying to obtain liquid wax gourd waste organic fertilizer.

[0030] (1) Isolation, purification and screening of degradation strains

[0031] 1) Preparation of decomposing agent 1: Ferment the thermophilic amylase Streptomyces to obtain a bacterial liquid, mix it with a mixture of whole wheat flour, leftover rice and cornmeal as a bacterial liquid adsorbent, and prepare a solid decomposing agent 1 for use; 2) Preparation of thermophilic cellulose degrading bacteria / thermophilic stearobacillus / lateral spore Bacillus / protein enzyme: Weigh 1g of the bacterial source sample in an inorganic salt liquid culture medium, and repeatedly culture for several generations until the enriched bacterial liquid with stable cellulose degradation effect is obtained. Take 1mL of the enriched bacterial liquid, dilute it to 10-9 in a gradient, and take 70μL of the diluted bacterial liquid and evenly spread it on a CMC solid plate, and invert and culture it at 28℃ for 3-7d. Select single colonies with different morphologies, inoculate them on a CMC solid culture medium, purify and culture them multiple times, and finally obtain pure strains.

[0032] (2) Construction and screening of microbial flora combined enzyme yeast agent, enzyme production conditions and degradation effect

[0033] The selected efficient degradation strains were inoculated into liquid culture medium and cultured at 28°C with shaking. The bacterial suspension of the non-antagonistic degradation strains was combined in equal proportions to prepare a composite bacterial suspension (thermophilic amylase Streptomyces 10 8 、Thermophilic cellulose degrading bacteria 10 8 、Bacillus stearothermophilus 10 8 、Protease 10 8 、Bacillus laterosporus 10 8 ) are respectively preserved according to a 1:1 strain ratio, respectively cultured according to a 1:1 strain ratio, respectively expanded according to a 1:1 original bacterial population ratio, respectively fermented in a liquid state according to a 1:1 strain population ratio, dried as a preparation, and made into a microbial flora combination enzyme yeast agent.

[0034] (3) Microbial flora combination enzyme yeast agent microbial organic fertilizer (solid) wax gourd vine (crushed) 100 parts, waste chicken feathers (crushed) 100 parts, durian shell 20-30 parts, tofu haws 20-30 parts, sugar cane mud 20-25 parts, acetic acid 2-5 parts.

[0035] (4) Microbial flora combination enzyme yeast agent organic fertilizer (liquid) 200 parts of crushed and concentrated juice of discarded wax gourd (discarded wax gourd, rind, seeds, and pulp), 20-25 parts of waste molasses liquid, and 50 parts of the homemade microbial flora combination enzyme yeast agent of step (1) are put into a 3t reactor for shearing, stirring, fermentation, composting, quality inspection, and drying to obtain liquid microbial organic fertilizer.

[0036] (5) Preparation of microbial functional seedling matrix: 100 parts of solid microbial organic fertilizer of waste feather powder fermentation product in a ratio of 1:1, 1 part of water retaining agent, 1 part of polyacrylamide (salt-tolerant), 1-2 parts of gibberellin 0.5mg / L, 2-4 parts of potassium dihydrogen phosphate, 1 part of carbendazim, put into a 3t reaction tank, shear and stir to prepare a salt-resistant and disease-proof seedling matrix. The germination index for wax gourd seedlings is about 80%, and the germination index for tomato seedlings is 100%, which significantly improves plant height, stem thickness, and aboveground fresh weight. It is resistant to repeated cropping, salt-tolerant, resistant to diseases and insect pests, retains water and fertilizer, has good air permeability and buffering properties, suitable bulk density, wide source, low cost, and good effect.

[0037] The wax gourd juice is prepared by crushing wax gourd, wax gourd peel, wax gourd pulp, and / or wax gourd seeds with a crusher.

[0038] The enzyme is prepared by using bran as the main raw material, adding bran and bean cake, and inoculating saccharifying Aspergillus and Rhizopus after water adjustment, steaming and cooling, and the inoculation amount is 0.25% to 0.35% of the raw material amount.

[0039] The present invention uses winter melon waste and waste chicken feathers as raw materials, so that the winter melon is rich in citrulline and protein, and the chicken feathers contain about 91% keratin, 9.64% nitrogen, 0.11% phosphorus, and 0.15% potassium, which is a nitrogen-based amino acid comprehensive fertilizer. Sugar mud contains protein, sugar, calcium, iron, zinc, magnesium and other elements. The above nutrients are fully maintained and utilized through homemade enzyme enzymolysis, promoting plant nutrient absorption and growth, and using various microorganisms in sugar mud to regulate soil flora, improve soil structure, increase soil fertility, and improve, neutralize, and correct the salinity and alkali content of saline-alkali land to achieve the environment required for crop growth.

[0040] The auxiliary material sugar clay of the present invention contains various elements such as protein, sugar, calcium, iron, zinc, magnesium, etc., which can be fully maintained and utilized to promote the nutrient absorption and growth of plants. Various microorganisms in the sugar clay are used to regulate soil flora, improve soil structure, increase soil fertility, improve and neutralize the content of coastal saline soil to achieve the environment required for rice growth.

[0041] Example 1

[0042] The steps of this embodiment are as follows: the wax gourd waste powder and waste chicken feathers are ground into a solid biological organic fertilizer for anti-salinity and alkali land, and the fertilizer is spread once at 500 kg / hm2. 2 Sunflowers sown on solid tidal flats using winter melon waste as salt-alkali resistant bio-organic fertilizer can grow normally, with plant heights reaching 50 to 100 cm.

[0043] Control group: Only the above-mentioned saline soil was replaced with normal soil, and the other conditions remained the same.

[0044] Location: Alaer, Xinjiang

[0045] Example 2

[0046] The steps of this embodiment are as follows: the wax gourd waste powder and waste chicken feathers are ground into a solid biological organic fertilizer for anti-salinity and alkaline land,

[0047] Broadcasting 550kg / hm2 once 2 Sunflowers sown with a 25cm cover on the soil grow more vigorously than those sown without a cover on the soil.

[0048] Control group: Only the above-mentioned saline soil was replaced with normal soil, and the other conditions remained the same.

[0049] Location: Alar, Xinjiang

[0050] Example 3

[0051] The steps of this embodiment are as follows: the wax gourd waste powder and waste chicken feathers are ground into a solid biological organic fertilizer for anti-salinity and alkaline land,

[0052] Apply once in saline-alkali land at 500 kg / hm 2 Winter melon waste anti-salinity and alkali land biological organic fertilizer solid field sowing wild forage seeds alfalfa can grow normally.

[0053] Control group: Only the above-mentioned saline-alkali soil was replaced with normal cultivated soil, and the other conditions remained the same.

[0054] Location: Ruoqiang County, Xinjiang

[0055] Example 4

[0056] Steps of this embodiment: wax gourd waste powder and waste chicken feathers are ground into salt-alkali-resistant biological organic fertilizer solid base fertilizer, base fertilizer, base fertilizer or thrown and spread

[0057] According to 600kg / hm 2 / times the same throwing, or repeating twice in a staggered manner, with an interval of 10-15 days. Planting Jinghe wolfberry on the beach in Ruoqiang County, Xinjiang, the plant height can reach 50-100cm.

[0058] Control group: Only the above-mentioned saline soil was replaced with normal soil, and the other conditions remained the same.

[0059] Location: Ruoqiang County, Xinjiang

[0060] Example 5

[0061] The steps of this embodiment are as follows: drip irrigation, watering, spraying of winter melon juice anti-salinity biological organic fertilizer liquid is repeated twice, or cross-pattern is repeated twice, with an interval of 10-15 days. Dilute 10-50 kg per mu at a concentration of 2%. Until the improvement reaches the saline-alkali land conditions below moderate, the dry weight of indica-japonica hybrid rice at heading stage and maturity, and the crop growth rate at jointing stage-heading stage and heading-maturity stage all grow normally.

[0062] Control group: Only the above-mentioned saline-alkali soil was replaced with normal soil, and the other conditions remained the same.

[0063] Location: Zhanjiang Beach

[0064] Example 6

[0065] The steps of this embodiment are as follows: spraying the winter melon juice anti-salinity bio-organic fertilizer liquid is repeated twice, with an interval of 10-15 days. Diluted at a concentration of 2%, 50-150 kg is applied per mu, and the leaf area index of the mature stage of indica-japonica hybrid rice, the photosynthetic potential at the heading stage and the mature stage are all normal, the leaf area index at the heading stage and the mature stage is larger, and the leaves remain green.

[0066] Control group: Only the above-mentioned saline soil was replaced with normal soil, and the other conditions remained the same.

[0067] The above description is only a preferred embodiment of the present invention and is not intended to limit the protection scope of the present invention.

Claims

1. A wax gourd waste microbial organic fertilizer, characterized in that: It comprises 20-50 parts of microbial flora combined enzyme yeast agent and 100-200 parts of winter melon waste organic fertilizer.

2. The wax gourd waste microbial organic fertilizer according to claim 1, characterized in that The winter melon waste organic fertilizer includes 100 parts of winter melon vines, 100 parts of waste chicken feathers, 20-30 parts of durian shells, 20-30 parts of tofu haws, 20-25 parts of sugarcane mud, and 2-5 parts of acetic acid.

3. Winter melon waste 0 microbial organic fertilizer according to claim 1, is characterized in that, The winter melon waste organic fertilizer comprises 200 parts of waste winter melon crushed juice and concentrated juice and 20-25 parts of waste molasses liquid.

4. The wax gourd waste microbial organic fertilizer according to any one of claims 1 to 3, characterized in that: The microbial flora combined enzyme yeast agent comprises 1-10 parts of thermophilic amylase Streptomyces, 1-10 parts of thermophilic cellulose degrading bacteria, 1-10 parts of thermophilic stearothermophilic Bacillus, 1-10 parts of protein enzyme, and 1-10 parts of Bacillus laterosporus.

5. The wax gourd waste microbial organic fertilizer according to claim 4, characterized in that, The waste wax gourd crushed juice concentrate is the crushed juice concentrate of waste wax gourd, rind, seeds and / or pulp.

6. A method for preparing wax gourd waste microbial organic fertilizer, characterized in that: The method includes: Obtain bacterial liquid, preserve the strains according to a 1:1 ratio, culture the strains according to a 1:1 ratio, expand the original bacterial population according to a 1:1 ratio, ferment the liquid according to a 1:1 ratio, and dry the preparation to prepare a microbial flora combined enzyme yeast agent; The prepared wax gourd waste organic fertilizer and microbial flora combined enzyme yeast are pre-treated by shearing and stirring in a 3t reactor, mixed evenly, and then fermented. After enzymatic hydrolysis and decomposition, they are filtered, separated into dry and wet parts, and dried to obtain wax gourd waste microbial organic fertilizer.

7. The method for preparing wax gourd waste microbial organic fertilizer according to claim 6, wherein The prepared wax gourd waste organic fertilizer specifically comprises: putting 100 parts of wax gourd vines, 100 parts of waste chicken feathers, 20-30 parts of durian shells, 20-30 parts of tofu haws, 20-25 parts of sugarcane mud and 2-5 parts of acetic acid into a 3t reaction tank, shearing, crushing and stirring, fermenting for 30-60 days, taking out the decomposed waste chicken feathers and wax gourd vine powder, stirring evenly, and naturally drying in the sun until the water content reaches 30%, so as to obtain solid wax gourd waste organic fertilizer.