Fluid system, control method, sequencing system and storage medium

Abstract

The invention discloses a fluid system, a control method, a sequencing system and a storage medium. The fluid system comprises a reaction device, a pipeline and a degassing device, the reaction device is used for providing a place for biochemical reaction, the pipeline is communicated with the reaction device and used for conveying liquid to the reaction device and / or receiving liquid flowing out of the reaction device, and the degassing device is connected with the pipeline and used for removing gas in the liquid in the pipeline. Therefore, gas in the liquid in the pipeline is removed through the degassing device, and bubbles formed in the reaction device can be reduced.

Description

Technical Field

[0001] The present invention relates to the field of gene sequencing, and in particular to a fluid system, a control method, a sequencing system and a storage medium. Background Art

[0002] Gene sequencing technology refers to the technical means of obtaining DNA or RNA base sequences through detection. The currently dominant sequencing technology is high-throughput sequencing technology. In the sequencing platform based on sequencing by synthesis to achieve high-throughput sequencing, the general process of gene sequencing includes fixing the nucleic acid sample to be tested on a flow cell in a manner such as hybridization; then using PCR amplification to form nucleic acid molecule clusters in the nucleic acid sample to be tested; then adding sequencing reagents, such as bases with fluorescent groups, polymerases, and primers, to the flow cell through a fluid system, and combining the bases with fluorescent groups with the bases on the nucleic acid sample to be tested through the principle of complementary base pairing; finally, the fluorescent group is excited to produce fluorescence through an optical imaging system and the fluorescence is collected to form an image, and base recognition is performed through the image, thereby realizing the base sequence determination of the nucleic acid sample to be tested.

[0003] However, if there is gas in the sequencing reagents and / or the nucleic acid samples to be tested, the gas will enter the flow cell along with the sequencing reagents and / or the nucleic acid samples to be tested, and bubbles will be formed in the flow cell, thereby affecting the quality of photography and further affecting the sequencing quality. Summary of the invention

[0004] The present application provides a fluid system, a control method, a sequencing system and a storage medium to solve at least one of the technical problems existing in the prior art.

[0005] On the one hand, the present application provides a fluid system, which includes a reaction device, a pipeline and a degassing device. The reaction device is used to provide a place for conducting biochemical reactions. The pipeline is connected to the reaction device, and the pipeline is used to transport liquid to the reaction device and / or receive liquid flowing out of the reaction device. The degassing device is connected to the pipeline, and the degassing device is used to remove gas from the liquid in the pipeline.

[0006] In this way, the gas in the liquid in the pipeline is removed by the degassing device, which can reduce or avoid the formation of bubbles in the reaction device.

[0007] In certain embodiments, the reaction device comprises a liquid inlet and a liquid outlet;

[0008] The pipeline comprises a first pipeline and a second pipeline, the first pipeline is communicated with the liquid inlet, the second pipeline is communicated with the liquid outlet, and the first pipeline and / or the second pipeline is connected to the degassing device.

[0009] In certain embodiments, the reaction device includes a liquid inlet and a liquid outlet, the pipeline includes a bypass pipeline, one end of the bypass pipeline is connected to the liquid inlet, and the other end is connected to the liquid outlet, and the bypass pipeline is connected to the degassing device.

[0010] In some embodiments, the degassing device includes a closed chamber, and the pipeline passes through the closed chamber. The portion of the pipeline located in the closed chamber can allow the gas in the liquid in the pipeline to pass through when the closed chamber is under negative pressure conditions, so that the gas in the liquid in the pipeline is removed after passing through the closed chamber.

[0011] In some embodiments, the degassing device includes a negative pressure device and an exhaust pipeline, one end of the exhaust pipeline is connected to the closed chamber, and the negative pressure device is arranged on the exhaust pipeline and is used to generate negative pressure in the closed chamber.

[0012] In certain embodiments, the degassing device includes a detector located between the negative pressure device and the closed chamber, the detector is connected to the exhaust pipeline, and the detector is used to detect the air pressure in the closed chamber through the exhaust pipeline.

[0013] In certain embodiments, the degassing device includes a multi-way valve disposed on the exhaust line and located between the detector and the negative pressure device, and the multi-way valve can connect the negative pressure device to the closed chamber or the atmosphere.

[0014] In another aspect, the present application provides a method for controlling degassing of liquid in a fluid system, the method comprising:

[0015] Determine whether the degassing device is normal;

[0016] When the degassing device is normal, the liquid in the control pipeline is degassed through the degassing device.

[0017] In some embodiments, determining whether the degassing device is normal includes:

[0018] The gas pressure in the closed chamber is obtained by using a detector;

[0019] Judge whether the degassing device is normal based on the change in air pressure.

[0020] In some embodiments, judging whether the degassing device is normal according to the change in air pressure includes:

[0021] Acquire a first air pressure in the sealed chamber at an initial moment;

[0022] obtaining a second air pressure in the sealed chamber at a preset time interval;

[0023] The air pressure change rate is calculated using the first air pressure, the second air pressure and a preset time interval;

[0024] Compare the air pressure change rate with the preset change rate to determine whether the degassing device is normal.

[0025] In some embodiments, comparing the air pressure change rate with a preset change rate to determine whether the degassing device is normal includes:

[0026] When the negative pressure device is working, if the air pressure change rate is greater than or equal to the preset change rate, the degassing device is judged to be normal; if the air pressure change rate is less than the preset change rate, the degassing device is judged to be abnormal.

[0027] In some embodiments, comparing the air pressure change rate with a preset change rate to determine whether the degassing device is normal includes:

[0028] When the negative pressure device stops working, if the air pressure change rate is less than or equal to the preset change rate, the degassing device is judged to be normal; if the air pressure change rate is greater than the preset change rate, the degassing device is judged to be abnormal.

[0029] In some embodiments, judging whether the degassing device is normal according to the change in air pressure includes:

[0030] When the negative pressure device stops working, obtaining a first moment when the air pressure is a first threshold, and obtaining a second moment when the air pressure is a second threshold, the second threshold being greater than the first threshold;

[0031] The time difference is calculated using the first moment and the second moment;

[0032] The time difference is compared with the preset time difference to determine whether the degassing device is normal.

[0033] In some embodiments, comparing the time difference with a preset time difference to determine whether the degassing device is normal includes:

[0034] If the time difference is greater than or equal to the preset time difference, the degassing device is judged to be normal; if the time difference is less than the preset time difference, the degassing device is judged to be abnormal.

[0035] In some embodiments, judging whether the degassing device is normal according to the change in air pressure includes:

[0036] When the negative pressure device stops working, the air pressure is recorded as the first threshold;

[0037] When the air pressure reaches a second threshold, the negative pressure device is activated;

[0038] If the air pressure is greater than the first threshold within the preset time, the degassing device is judged to be abnormal; if the air pressure is less than or equal to the first threshold within the preset time, the degassing device is judged to be normal and the second threshold is greater than the first threshold.

[0039] In some embodiments, judging whether the degassing device is normal according to the change in air pressure includes:

[0040] When the negative pressure device stops working, the air pressure is recorded as the first threshold;

[0041] At a preset time interval, determining whether the air pressure in the sealed chamber is greater than a first threshold;

[0042] If the air pressure in the sealed chamber is greater than a first threshold, obtaining a rate of change of the air pressure;

[0043] If the rate of change of the air pressure is greater than the preset rate of change, the degassing device is judged to be abnormal; otherwise, the degassing device is judged to be normal.

[0044] In certain embodiments, the method further comprises:

[0045] If the degassing device is normal, start the negative pressure device;

[0046] If the degassing device is abnormal, the negative pressure device will not be started.

[0047] In certain embodiments, the degassing device includes a first degassing device, and the first pipeline is connected to the first degassing device;

[0048] When the degassing device is normal, the liquid in the control pipeline is degassed through the degassing device, including:

[0049] The liquid in the first pipeline is controlled to be degassed through the first degassing device and the degassed liquid is allowed to enter the reaction device.

[0050] In certain embodiments, the degassing device includes a second degassing device, and the second pipeline is connected to the second degassing device;

[0051] When the degassing device is normal, the liquid in the control pipeline is degassed through the degassing device, including:

[0052] The liquid flowing out of the reaction device and entering the second pipeline is controlled to be degassed through the second degassing device.

[0053] In certain embodiments, the degassing device comprises a third degassing device,

[0054] The pipeline includes a bypass pipeline, one end of the bypass pipeline is connected to the liquid inlet, the other end of the bypass pipeline is connected to the liquid outlet, and the bypass pipeline is connected to the third degassing device;

[0055] When the degassing device is normal, the liquid in the control pipeline is degassed through the degassing device, including:

[0056] The liquid flowing out of the reaction device is controlled to enter the bypass pipeline, and the liquid in the bypass pipeline is allowed to enter the third degassing device for degassing.

[0057] In certain embodiments, the method further comprises:

[0058] The degassed liquid in the bypass pipeline is allowed to enter the reaction device.

[0059] On the other hand, the present application provides a sequencing system, comprising the above-mentioned fluid system.

[0060] In addition, the present application also provides a computer storage medium, which enables the processor to implement any method of the above-mentioned implementation mode when the computer program is executed by the processor.

[0061] Additional aspects and advantages of the present invention will be given in part in the following description and in part will be obvious from the following description, or will be learned through practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0062] The above and / or additional aspects and advantages of the present invention will become apparent and easily understood from the description of the embodiments in conjunction with the following drawings, in which:

[0063] Figure 1 is a schematic structural diagram of a fluid system according to an embodiment of the present invention;

[0064] Figure 2 is a schematic structural diagram of a degassing device according to an embodiment of the present invention;

[0065] Figure 3 is a schematic structural diagram of a degassing device according to an embodiment of the present invention;

[0066] Figure 4 is a schematic flow chart of a method according to an embodiment of the present invention;

[0067] Figure 5 is a schematic flow chart of a method according to an embodiment of the present invention;

[0068] Figure 6 is a schematic flow chart of a method according to an embodiment of the present invention;

[0069] Figure 7 It is a schematic flow chart of the method according to the embodiment of the present invention.

[0070] Explanation of the reference numerals: 100, fluid system; 10, reaction device; 11, liquid inlet; 12, liquid outlet; 20, pipeline; 21, first pipeline; 22, second pipeline; 23, bypass pipeline; 30, degassing device; 31, closed chamber; 32, negative pressure device; 33, exhaust pipeline; 34, detector; 35, three-way connector; 36, multi-way valve; 37, first degassing device; 38, second degassing device; 39, third degassing device. DETAILED DESCRIPTION

[0071] The embodiments of the present invention are described in detail below, examples of which are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention, and cannot be understood as limiting the present invention.

[0072] In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise" and the like indicate positions or positional relationships based on the positions or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first" and "second" are only used for descriptive purposes, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Therefore, the features defined as "first" and "second" may explicitly or implicitly include one or more of the features. In the description of the present invention, the meaning of "multiple" is two or more, unless otherwise clearly and specifically defined.

[0073] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection, an electrical connection, or mutual communication; it can be a direct connection, or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0074] In the present invention, unless otherwise clearly specified and limited, a first feature being "above" or "below" a second feature may include that the first and second features are in direct contact, or may include that the first and second features are not in direct contact but are in contact through another feature between them. Moreover, a first feature being "above", "above" and "above" a second feature includes that the first feature is directly above and obliquely above the second feature, or simply indicates that the first feature is higher in level than the second feature. A first feature being "below", "below" and "below" a second feature includes that the first feature is directly below and obliquely below the second feature, or simply indicates that the first feature is lower in level than the second feature.

[0075] The disclosure below provides many different embodiments or examples to realize different structures of the present invention. In order to simplify the disclosure of the present invention, the parts and settings of specific examples are described below. Of course, they are only examples, and the purpose is not to limit the present invention. In addition, the present invention can repeat reference numbers and / or reference letters in different examples, and this repetition is for the purpose of simplicity and clarity, which itself does not indicate the relationship between the various embodiments and / or settings discussed. In addition, the present invention provides various specific examples of processes and materials, but those of ordinary skill in the art can be aware of the application of other processes and / or the use of other materials.

[0076] See also Figure 1 The present application provides a fluid system 100, which includes a reaction device 10, a pipeline 20 and a degassing device 30. The reaction device 10 is used to provide a place for performing biochemical reactions. The pipeline 20 is connected to the reaction device 10. The pipeline 20 is used to transport liquid to the reaction device 10 and / or receive liquid flowing out of the reaction device 10. The degassing device 30 is connected to the pipeline 20 and is used to remove gas from the liquid in the pipeline 20.

[0077] In this way, the gas in the liquid in the pipeline 20 is removed by the degassing device 30 , so that the formation of bubbles in the reaction device 10 can be reduced.

[0078] Specifically, the reaction device 10 may be a flow cell, which may also be called a chip. The chip includes a plurality of fluid channels, each of which has a space for accommodating liquids and can accommodate samples to be tested and reagents, so that the samples to be tested and the reagents undergo a biochemical reaction.

[0079] The pipeline 20 may be a pipe for transmitting liquid, the cross-sectional shape of the pipeline 20 may be circular, and the length and diameter of the pipeline 20 may be set according to actual needs. The degassing device 30 may be connected to a plurality of pipelines 20 to remove gas from the liquid in the plurality of pipelines 20. The gas in the liquid may be gas originally existing in the liquid, for example, a certain inhibitory gas is added to the reagent to keep the reagent stable, or gas precipitated from the liquid due to excessive negative pressure in the pipeline 20, or gas dissolved in the liquid.

[0080] See also Figure 1 In some embodiments, the reaction device 10 includes a liquid inlet 11 and a liquid outlet 12, the pipeline 20 includes a first pipeline 21 and a second pipeline 22, the first pipeline 21 is connected to the liquid inlet 11, the second pipeline 22 is connected to the liquid outlet 12, and the first pipeline 21 and / or the second pipeline 22 are connected to the degassing device 30.

[0081] Specifically, the first pipeline 21 may be connected to the degassing device 30 , the second pipeline 22 may be connected to the degassing device 30 , or both the first pipeline 21 and the second pipeline 22 may be connected to the degassing device 30 .

[0082] When transporting liquid to the reaction device 10, the liquid can enter the reaction device 10 from the liquid inlet 11 after being degassed by the degassing device 30 in the first pipeline 21, or can enter the reaction device 10 from the liquid outlet 12 after being degassed by the degassing device 30 in the second pipeline 22. In this way, the liquid can be degassed in advance before flowing into the reaction device 10, thereby reducing or avoiding the formation of bubbles in the reaction device 10.

[0083] When receiving the liquid flowing out of the reaction device 10, the liquid can flow out of the reaction device 10 from the liquid outlet 12 and be stored in the second pipeline 22 after being degassed by the degassing device 30, and then enter the reaction device 10 from the liquid outlet 12 after being degassed by the degassing device 30 in the second pipeline 22, so as to be reused and reduce costs.

[0084] The liquid inlet 11 and the liquid outlet 12 are respectively located at the two ends of the fluid channel of the reaction device 10. The number of fluid channels can be multiple, each fluid channel includes a liquid inlet 11 and a liquid outlet 12, the number of first pipelines 21 and second pipelines 22 can be multiple, and the multiple fluid channels are arranged in a one-to-one correspondence with the multiple first pipelines 21 and the multiple second pipelines 22.

[0085] See also Figure 1 In some embodiments, the pipeline 20 includes a bypass pipeline 23 , one end of the bypass pipeline 23 is connected to the liquid inlet 11 , and the other end is connected to the liquid outlet 12 , and the bypass pipeline 23 is connected to the degassing device 30 .

[0086] Specifically, the end of the bypass line 23 connected to the liquid inlet 11 can be located between the liquid inlet 11 and the degassing device 30 of the first line 21, and the degassing device 30 of the second line 22 can be located between the end of the bypass line 23 connected to the liquid outlet 12 and the liquid outlet 12.

[0087] The liquid flowing out of the reaction device 10 can be stored in the bypass line 23 after being degassed by the degassing device 30 in the second line 22, and then enter the first line 21 after being degassed by the degassing device 30 in the bypass line 23, and enter the reaction device 10 from the liquid inlet 11. The liquid can also enter the bypass line 23 after being degassed by the degassing device 30 in the second line 22, enter the first line 21 after being degassed by the degassing device 30 in the bypass line 23, and then be stored in the first line 21 or a storage container after being degassed by the degassing device 30 in the first line 21, and then enter the reaction device 10 from the liquid inlet 11 after being degassed by the degassing device 30 in the first line 21. Compared with the liquid flowing back from the second line 22 to the first line 21 through the reaction device 10, the liquid refluxes from the bypass line 23, which can reduce the flow resistance of the liquid, increase the flow rate of the liquid, and thus improve the recovery efficiency of the liquid.

[0088] During the process of liquid recycling and reuse, the liquid can be degassed through multiple degassing devices 30, thereby increasing the effective degassing area of ​​the pipeline 20 and improving the degassing effect. At the same time, the length or area of ​​the pipeline 20 can be reduced, the use of materials can be reduced, and the cost of the fluid system 100 and the flow resistance of the liquid can be reduced.

[0089] See also Figure 2 In some embodiments, the degassing device 30 includes a closed chamber 31, and the pipeline 20 passes through the closed chamber 31. The portion of the pipeline 20 located in the closed chamber 31 can allow the gas in the liquid in the pipeline 20 to pass through when the closed chamber 31 is under negative pressure conditions, so that the gas in the liquid in the pipeline 20 can be removed after passing through the closed chamber 31.

[0090] Specifically, the closed chamber 31 can be a closed space formed by polyvinyl chloride. The closed chamber 31 can be in a rectangular shape, a cylindrical shape, etc. The pipeline 20 passing through the closed chamber 31 can be the pipeline 20 passing through the same side wall of the closed chamber 31, or the pipeline 20 can pass through two adjacent side walls of the closed chamber 31, or the pipeline 20 can pass through two opposite side walls of the closed chamber 31.

[0091] The portion of the pipeline 20 located in the sealed chamber 31 may be a Teflon tube, which has the characteristics of being air-permeable and water-tight. The gas in the liquid can be discharged from the Teflon tube under the action of negative pressure when passing through the sealed chamber 31, and the discharged gas is located in the sealed chamber 31. The diameter of the Teflon tube may be consistent with the diameter of the first pipeline 21, the second pipeline 22 or the bypass pipeline 23, and the length of the Teflon tube may be set according to the content of the gas in the liquid. The negative pressure condition is the pressure range in which the gas in the sealed chamber 31 can cause the gas in the liquid in the pipeline 20 to pass through.

[0092] See also Figure 2In some embodiments, the degassing device 30 includes a negative pressure device 32 and an exhaust pipe 33, one end of the exhaust pipe 33 is connected to the closed chamber 31, and the negative pressure device 32 is arranged on the exhaust pipe 33 and is used to generate negative pressure in the closed chamber 31.

[0093] In this way, the negative pressure device 32 forms a negative pressure in the sealed chamber 31 , so that the gas in the liquid in the pipeline 20 can be discharged after passing through the sealed chamber 31 .

[0094] Specifically, the negative pressure device 32 can be a vacuum pump, including but not limited to a water ring pump, a reciprocating pump, a sliding valve pump, a rotary vane pump, a Roots pump and a diffusion pump. The negative pressure device 32 sucks away the air in the closed chamber 31 to generate negative pressure in the closed chamber 31.

[0095] The exhaust pipeline 33 can transport the gas in the closed chamber 31 to the negative pressure device 32, and then discharge it to the atmosphere through the negative pressure device 32, so that a negative pressure condition is formed in the closed chamber 31. The gas in the closed chamber 31 can be the gas removed from the first pipeline 21, the second pipeline 22 or the bypass pipeline 23.

[0096] See also Figure 2 In some embodiments, the degassing device 30 includes a detector 34 located between the negative pressure device 32 and the closed chamber 31 , and the detector 34 is connected to the exhaust pipe 33 , and the detector 34 is used to detect the air pressure in the closed chamber 31 through the exhaust pipe 33 .

[0097] Specifically, the detector 34 may be an air pressure sensor, which is a device that converts pressure into an electrical signal using principles such as piezoresistive, capacitive, piezoelectric or crystal resonance. The detector 34 may be connected to the exhaust pipe 33 via a three-way connector 35 .

[0098] By detecting the air pressure in the sealed chamber 31 through the detector 34, it can be determined whether the air pressure in the sealed chamber 31 reaches the negative pressure condition. When the air pressure in the sealed chamber 31 does not reach the negative pressure condition, the degassing device 30 is started, and when the air pressure in the sealed chamber 31 reaches the negative pressure condition, the negative pressure device 32 is closed. By adjusting the working state of the negative pressure device 32, the sealed chamber 31 is maintained in a negative pressure state, thereby improving the degassing efficiency of the degassing device 30, and at the same time, the opening and closing time of the negative pressure device 32 can be controlled, so that the use frequency of the negative pressure device 32 can be effectively reduced, thereby increasing the service life of the negative pressure device 32.

[0099] See also Figure 3 In some embodiments, the degassing device 30 includes a multi-way valve 36 disposed on the exhaust line 33 and located between the detector 34 and the negative pressure device 32, and the multi-way valve 36 can connect the negative pressure device 32 to the closed chamber 31 or the atmosphere.

[0100] Specifically, the multi-way valve 36 may be a three-way valve, and the three valve ports of the three-way valve are connected to the negative pressure device 32, the closed chamber 31 and the atmosphere respectively. In one embodiment, a negative pressure device 32 without a check function may be selected. When the negative pressure device 32 is working, the multi-way valve 36 connects the negative pressure device 32 and the closed chamber 31, so that the negative pressure device 32 sucks away the air in the closed chamber 31 to form a negative pressure; when the negative pressure device 32 stops working, the multi-way valve 36 connects the negative pressure device 32 with the atmosphere, which can reduce the risk of excessive air pressure in the closed chamber 31 when the negative pressure device 32 is started, resulting in the inability to start the negative pressure device 32 or difficulty in starting, thereby shortening the service life of the negative pressure device 32.

[0101] In another embodiment, a negative pressure device 32 with a one-way check function can be selected to reduce the use of solenoid valves, reduce the cost of the fluid system 100, and avoid solenoid valve failure, thereby improving the reliability of the degassing device 30.

[0102] See also Figure 4 The present application provides a method for controlling degassing of liquid in a fluid system 100, the method comprising:

[0103] S10, determining whether the degassing device 30 is normal;

[0104] S20 , when the degassing device 30 is operating normally, the liquid in the control pipeline 20 is degassed through the degassing device 30 .

[0105] Specifically, whether the degassing device 30 is normal can be determined before the fluid system 100 is activated or during the operation of the fluid system 100. The degassing device 30 is normal, which means that all components in the degassing device 30 are normal, such as the airtightness of the sealed chamber 31 is normal, the airtightness of the exhaust pipe 33 is normal, the negative pressure device 32 works normally, and the detector 34 works normally. The degassing device 30 is abnormal, which means that one or more components in the degassing device 30 are abnormal, such as the airtightness of the sealed chamber 31 is abnormal, the airtightness of the exhaust pipe 33 is abnormal, the negative pressure device 32 works abnormally, or the detector 34 works abnormally.

[0106] In this way, whether the degassing device 30 is activated is determined by judging whether the degassing device 30 is normal.

[0107] See also Figure 5 In some embodiments, determining whether the degassing device 30 is normal (step S10) includes:

[0108] S100, using the detector 34 to obtain the air pressure in the sealed chamber 31;

[0109] S200, judging whether the degassing device 30 is normal according to the change of air pressure.

[0110] Specifically, before using the detector 34 to obtain the air pressure in the sealed chamber 31 , the detector 34 may be tested first to ensure that the detector 34 is normal.

[0111] The change in air pressure can be the amount of change in air pressure within a certain period of time, or the time difference used for the air pressure to change by a certain amount. Whether the degassing device 30 is normal can be determined when the negative pressure device 32 is working, or whether the degassing device 30 is normal can be determined when the negative pressure device 32 stops working. In this way, whether the degassing device 30 is normal can be monitored during the entire operation of the fluid system 100 to fully remove the gas in the liquid in the pipeline 20.

[0112] See also Figure 6 In some embodiments, judging whether the degassing device 30 is normal according to the change of air pressure (step S200) includes:

[0113] S210, obtaining a first air pressure in the sealed chamber 31 at an initial moment;

[0114] S220, obtaining a second air pressure in the sealed chamber 31 at a preset time interval;

[0115] S230, calculating a pressure change rate using the first pressure, the second pressure, and a preset time interval;

[0116] S240, comparing the air pressure change rate with a preset change rate to determine whether the degassing device 30 is normal.

[0117] Specifically, the initial moment may be the moment when the negative pressure device 32 starts working, or the moment when the negative pressure device 32 stops working, and the preset time interval may be the working duration of the negative pressure device 32, or the stopping duration of the negative pressure device 32.

[0118] The air pressure change rate may be a ratio of the difference between the second air pressure and the first air pressure to a preset time interval, and the preset change rate is the change rate of the air pressure in the sealed chamber 31 within the preset time interval when the degassing device 30 is normal. The preset change rate may be a range, and when the air pressure change rate is within the range, the degassing device 30 is judged to be normal, and when the air pressure change rate is outside the range, the degassing device 30 is judged to be abnormal.

[0119] In this way, by calculating the rate of change of the air pressure within a preset time interval and comparing the rate of change of the air pressure with the preset rate of change, it is possible to objectively and accurately determine whether the degassing device 30 is normal.

[0120] In one embodiment, the air pressure difference at a preset time interval can be calculated using the first air pressure and the second air pressure, and then the air pressure difference at the preset time interval is compared with the preset air pressure difference at the preset time interval to determine whether the degassing device 30 is normal.

[0121] In some embodiments, comparing the air pressure change rate with a preset change rate to determine whether the degassing device 30 is normal includes:

[0122] When the negative pressure device 32 is working, if the air pressure change rate is greater than or equal to the preset change rate, the degassing device 30 is judged to be normal; if the air pressure change rate is less than the preset change rate, the degassing device 30 is judged to be abnormal.

[0123] Specifically, when the negative pressure device 32 is working, the air pressure change rate is greater than or equal to the preset change rate, which means that the air pressure in the closed chamber 31 has reached the negative pressure condition within the preset time interval, and the air tightness of the closed chamber 31, the air tightness of the exhaust pipe 33 and the negative pressure device 32 are all normal. Therefore, it can be judged that the degassing device 30 is normal.

[0124] If the air pressure change rate is less than the preset change rate, it means that the air pressure in the closed chamber 31 has not reached the negative pressure condition within the preset time interval, and the air tightness of the closed chamber 31, the air tightness of the exhaust pipe 33 or the negative pressure device 32 is abnormal. Therefore, it can be judged that the degassing device 30 is abnormal.

[0125] In some embodiments, comparing the air pressure change rate with a preset change rate to determine whether the degassing device 30 is normal includes:

[0126] When the negative pressure device 32 stops working, if the air pressure change rate is less than or equal to the preset change rate, the degassing device 30 is judged to be normal; if the air pressure change rate is greater than the preset change rate, the degassing device 30 is judged to be abnormal.

[0127] Specifically, when the negative pressure device 32 stops working, the air pressure change rate is less than or equal to the preset change rate, which means that the air tightness of the closed chamber 31 and the air tightness of the exhaust pipe 33 are normal, so it can be determined that the degassing device 30 is normal.

[0128] If the air pressure change rate is greater than the preset change rate, it means that the air tightness of the sealed chamber 31 or the air tightness of the exhaust pipe 33 is abnormal, so it can be determined that the degassing device 30 is abnormal.

[0129] See also Figure 7 In some embodiments, judging whether the degassing device 30 is normal according to the change of air pressure (step S200) includes:

[0130] S250, when the negative pressure device 32 stops working, obtaining a first moment when the air pressure is a first threshold, and obtaining a second moment when the air pressure is a second threshold, the second threshold being greater than the first threshold;

[0131] S260, calculating a time difference using the first moment and the second moment;

[0132] S270, comparing the time difference with a preset time difference to determine whether the degassing device 30 is normal.

[0133] Specifically, the first threshold value may be the lower limit value of the air pressure in the sealed chamber 31 reaching the negative pressure condition, and the second threshold value may be the upper limit value of the air pressure in the sealed chamber 31 reaching the negative pressure condition, and both the upper limit value and the lower limit value are negative values.

[0134] The time difference may be the difference between the first moment and the second moment, and the preset time difference is the time taken for the air pressure in the sealed chamber 31 to change from the first threshold to the second threshold when the degassing device 30 is normal. The preset time difference may be a range, and when the time difference for the air pressure to change from the first threshold to the second threshold is within the range, the degassing device 30 is judged to be normal, and when the time difference is outside the range, the degassing device 30 is judged to be abnormal.

[0135] In this way, by comparing the time difference when the air pressure changes from the first threshold to the second threshold with the preset time difference, it is possible to objectively and accurately determine whether the degassing device 30 is normal, and the calculation steps can be simplified to make the calculation process simple and fast, thereby improving the judgment efficiency.

[0136] In some embodiments, comparing the time difference between the change of the air pressure from the first threshold value to the second threshold value with the preset time difference to determine whether the degassing device 30 is normal includes:

[0137] If the time difference when the air pressure changes from the first threshold to the second threshold is greater than or equal to the preset time difference, the degassing device 30 is judged to be normal; if the time difference when the air pressure changes from the first threshold to the second threshold is less than the preset time difference, the degassing device 30 is judged to be abnormal.

[0138] Specifically, when the negative pressure device 32 stops working, the time difference for the air pressure to change from the first threshold to the second threshold is greater than or equal to the preset time difference, which means that the air pressure in the closed chamber 31 is maintained at a negative pressure condition within the preset time difference, and the air tightness of the closed chamber 31 and the air tightness of the exhaust pipe 33 are normal, so it can be judged that the degassing device 30 is normal.

[0139] If the time difference for the air pressure to change from the first threshold to the second threshold is less than the preset time difference, it means that the air pressure in the closed chamber 31 has not reached the negative pressure condition within the preset time difference, and the air tightness of the closed chamber 31 or the air tightness of the exhaust pipe 33 is abnormal. Therefore, it can be judged that the degassing device 30 is abnormal.

[0140] In some embodiments, judging whether the degassing device 30 is normal according to the change of air pressure includes:

[0141] When the negative pressure device 32 stops working, the recorded air pressure is the first threshold value;

[0142] When the air pressure reaches a second threshold, the negative pressure device 32 is activated;

[0143] If the air pressure is greater than the first threshold within the preset time, the degassing device 30 is judged to be abnormal; if the air pressure is less than or equal to the first threshold within the preset time, the degassing device 30 is judged to be normal and the second threshold is greater than the first threshold.

[0144] Specifically, when the negative pressure device 32 is working, the air pressure is greater than the first threshold value within the preset time, which means that the gas pressure in the closed chamber 31 has not reached the negative pressure condition within the preset time difference, and the airtightness of the closed chamber 31, the airtightness of the exhaust pipe 33 or the negative pressure device 32 is abnormal. Therefore, it can be judged that the degassing device 30 is abnormal.

[0145] If the air pressure is less than or equal to the first threshold within the preset time, it means that the gas pressure in the closed chamber 31 reaches the negative pressure condition within the preset time difference, and the air tightness of the closed chamber 31, the air tightness of the exhaust pipe 33 and the negative pressure device 32 are all normal. Therefore, it can be judged that the degassing device 30 is normal.

[0146] In some embodiments, judging whether the degassing device 30 is normal according to the change of air pressure includes:

[0147] When the negative pressure device 32 stops working, the recorded air pressure is the first threshold value;

[0148] At a preset time interval, determining whether the air pressure in the sealed chamber 31 is greater than a first threshold;

[0149] If the air pressure in the sealed chamber 31 is greater than the first threshold, the rate of change of the air pressure is obtained;

[0150] If the rate of change of the air pressure is greater than the preset rate of change, the degassing device 30 is judged to be abnormal; otherwise, the degassing device 30 is judged to be normal.

[0151] Specifically, when the negative pressure device 32 stops working, the air pressure in the closed chamber 31 reaches the lower limit of the negative pressure condition. As the gas in the liquid in the pipeline 20 enters the closed chamber 31 from the pipeline 20 under the action of negative pressure, the air pressure in the closed chamber 31 rises.

[0152] If the pressure change rate is greater than the preset change rate, it means that gas has entered the closed chamber 31 from the external environment, which further indicates that the airtightness of the closed chamber 31 or the airtightness of the exhaust pipe 33 is abnormal. Therefore, it can be determined that the degassing device 30 is abnormal.

[0153] If the air pressure change rate is less than or equal to the preset change rate, it means that the air tightness of the sealed chamber 31 and the air tightness of the exhaust pipe 33 are normal, so it can be determined that the degassing device 30 is normal.

[0154] In certain embodiments, the method further comprises:

[0155] If the degassing device 30 is normal, the negative pressure device 32 is started;

[0156] If the degassing device 30 is abnormal, the negative pressure device 32 is not activated.

[0157] Specifically, before activating the fluid system 100, the negative pressure device 32 can be started to determine whether the degassing device 30 is normal. If the degassing device 30 is normal, the negative pressure device 32 is started during the operation of the fluid system 100. If the degassing device 30 is abnormal, the negative pressure device 32 is not started during the operation of the fluid system 100.

[0158] During the operation of the fluid system 100, it can be determined at regular intervals whether the degassing device 30 is normal. If the degassing device 30 is normal, the degassing device 30 is started when the air pressure in the sealed chamber 31 does not reach the negative pressure condition, and the negative pressure device 32 is closed when the air pressure in the sealed chamber 31 reaches the negative pressure condition. If the degassing device 30 is abnormal, the negative pressure device 32 is not started during the subsequent operation of the fluid system 100.

[0159] In one embodiment, the detector 34 can be connected to a computer device, and the computer can determine whether the degassing device 30 is normal according to the change of air pressure. When the degassing device 30 is abnormal, the computer can issue an alarm and shut down the negative pressure device 32 that is working.

[0160] In this way, starting the degassing device 30 when the degassing device 30 is normal can effectively remove the gas in the liquid in the pipeline 20, and shutting down the degassing device 30 when the degassing device 30 is abnormal can reduce the waste of power resources and the consumption of the life of the negative pressure device 32.

[0161] In some embodiments, the degassing device 30 includes a first degassing device 37, and the first pipeline 21 is connected to the first degassing device 37;

[0162] When the degassing device 30 is normal, the liquid in the control pipeline 20 is degassed through the degassing device 30, including:

[0163] The liquid in the first pipeline 21 is controlled to be degassed through the first degassing device 37 and the degassed liquid is allowed to enter the reaction device 10 .

[0164] Specifically, a negative pressure condition is formed in the closed chamber 31 in the first degassing device 37, and the gas in the liquid in the first pipeline 21 flows out from the first pipeline 21 into the closed chamber 31 when passing through the closed chamber 31. The gas in the closed chamber 31 is then discharged into the atmosphere from the exhaust pipeline 33, and the liquid in the first pipeline 21 that passes through the closed chamber 31 enters the reaction device 10 from the liquid inlet 11.

[0165] In this way, the gas in the liquid in the first pipeline 21 can be removed, and the formation of bubbles in the reaction device 10 can be reduced or avoided.

[0166] In some embodiments, the degassing device 30 includes a second degassing device 38, and the second pipeline 22 is connected to the second degassing device 38;

[0167] When the degassing device 30 is normal, the liquid in the control pipeline 20 is degassed through the degassing device 30, including:

[0168] The liquid flowing out of the reaction device 10 and entering the second pipeline 22 is controlled to be degassed by the second degassing device 38 .

[0169] Specifically, the selection of components in the second degassing device 38 may be the same as or different from that of the first degassing device 37. A negative pressure condition is formed in the sealed chamber 31 in the second degassing device 38, and the gas in the liquid in the second pipeline 22 flows out from the second pipeline 22 into the sealed chamber 31 when passing through the sealed chamber 31, and the gas in the sealed chamber 31 is then discharged to the atmosphere from the exhaust pipeline 33, and the liquid in the second pipeline 22 that passes through the sealed chamber 31 can be stored in the second pipeline 22 or the bypass pipeline 23 for reuse.

[0170] In this way, the gas in the liquid in the second pipeline 22 can be removed, reducing or avoiding the formation of bubbles in the reaction device 10 when the liquid is reused.

[0171] In some embodiments, the degassing device 30 includes a third degassing device 39, and the bypass line 23 is connected to the third degassing device 39;

[0172] When the degassing device 30 is normal, the liquid in the control pipeline 20 is degassed through the degassing device 30, including:

[0173] The liquid flowing out of the reaction device 10 is controlled to enter the bypass pipeline 23, and the liquid in the bypass pipeline 23 is made to enter the third degassing device 39 for degassing.

[0174] Specifically, the selection of components in the third degassing device 39 may be the same as or different from that of the first degassing device 37 or the second degassing device 38. The liquid degassed by the second degassing device 38 may enter the bypass line 23, and a negative pressure condition is formed in the sealed chamber 31 in the third degassing device 39. The gas in the liquid in the bypass line 23 flows out from the bypass line 23 into the sealed chamber 31 when passing through the sealed chamber 31. The gas in the sealed chamber 31 is then discharged from the exhaust line 33 to the atmosphere. The liquid in the bypass line 23 that passes through the sealed chamber 31 may be stored in the bypass line 23 for reuse.

[0175] In this way, the gas in the liquid in the bypass line 23 can be removed, and the formation of bubbles in the reaction device 10 when the liquid is reused can be reduced or avoided.

[0176] In certain embodiments, the method further comprises:

[0177] The degassed liquid in the bypass line 23 enters the reaction device 10 .

[0178] Specifically, the liquid degassed by the third degassing device 39 enters the first pipeline 21, and then enters the reaction device 10 from the liquid inlet 11. In this way, the liquid can be reused and the cost can be reduced.

[0179] An embodiment of the present application provides a sequencing system, comprising the above-mentioned fluid system 100.

[0180] An embodiment of the present application provides a computer storage medium. When a computer program is executed by a processor, the processor implements the method for degassing liquid in a control fluid system 100 of any of the above embodiments.

[0181] Specifically, in one embodiment, the processor may be a central processing unit (CPU). The processor may also be other general-purpose processors, digital signal processors (DSP), application-specific integrated circuits (ASIC), field-programmable gate arrays (FPGA) or other programmable logic devices, discrete gate or transistor logic devices, discrete hardware components, or a combination of the above chips.

[0182] The computer program can be stored in the memory. The memory, as a non-transitory computer-readable storage medium, can be used to store non-transitory software programs, non-transitory computer executable programs and modules, such as the program instructions / modules corresponding to the methods in the above method embodiments. The processor executes various functional applications and data processing of the processor by running the non-transitory software programs, instructions and modules stored in the memory, that is, realizing the control method in the above method embodiments.

[0183] The storage medium may include, but is not limited to, various media that can store computer programs, such as a USB flash drive, a read-only memory (ROM), a random access memory (RAM), a mobile hard disk, a magnetic disk, or an optical disk.

[0184] In the description of this specification, the description with reference to the terms "one embodiment", "certain embodiments", "illustrative embodiments", "examples", "specific examples", or "some examples" means that the specific features, structures, materials, or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials, or characteristics described may be combined in any one or more embodiments or examples in a suitable manner.

[0185] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the claims and their equivalents.

Claims

1. A fluid system, characterized in that: include: A reaction device, wherein the reaction device is used to provide a place for performing a biochemical reaction; a pipeline, the pipeline being in communication with the reaction device, the pipeline being used to transport liquid to the reaction device and / or receive liquid flowing out of the reaction device; A degassing device is connected to the pipeline and is used to remove gas from the liquid in the pipeline.

2. The fluid system according to claim 1, characterized in that The reaction device comprises a liquid inlet and a liquid outlet; The pipeline comprises a first pipeline and a second pipeline, the first pipeline is communicated with the liquid inlet, the second pipeline is communicated with the liquid outlet, and the first pipeline and / or the second pipeline are connected to the degassing device; Optionally, the reaction device comprises a liquid inlet and a liquid outlet, the pipeline comprises a bypass pipeline, one end of the bypass pipeline is connected to the liquid inlet, and the other end is connected to the liquid outlet, and the bypass pipeline is connected to the degassing device.

3. The fluid system according to claim 1 or 2, characterized in that: The degassing device comprises a sealed chamber, the pipeline passes through the sealed chamber, and the portion of the pipeline located in the sealed chamber can allow the gas in the liquid in the pipeline to pass through when the sealed chamber is under negative pressure, so that the gas in the liquid in the pipeline is removed after passing through the sealed chamber; Optionally, the degassing device comprises a negative pressure device and an exhaust pipeline, one end of the exhaust pipeline is connected to the closed chamber, and the negative pressure device is arranged on the exhaust pipeline and is used to generate negative pressure in the closed chamber; Optionally, the degassing device comprises a detector located between the negative pressure device and the closed chamber, the detector being in communication with the exhaust pipeline, and the detector being used to detect the air pressure in the closed chamber through the exhaust pipeline; Optionally, the degassing device comprises a multi-way valve disposed on the exhaust pipeline and located between the detector and the negative pressure device, and the multi-way valve enables the negative pressure device to communicate with the closed chamber or the atmosphere.

4. A method for controlling the degassing of liquid in a fluid system, characterized in that: The fluid system comprises: A reaction device, wherein the reaction device is used to provide a place for performing a biochemical reaction; a pipeline, the pipeline being in communication with the reaction device, the pipeline being used to transport liquid to the reaction device and / or receive liquid flowing out of the reaction device; A degassing device, the degassing device is connected to the pipeline, and the degassing device is used to remove gas from the liquid in the pipeline; The method comprises: Determining whether the degassing device is normal; When the degassing device is operating normally, the liquid in the pipeline is controlled to pass through the degassing device for degassing.

5. The method according to claim 4, characterized in that The degassing device comprises a sealed chamber, the pipeline passes through the sealed chamber, and the portion of the pipeline located in the sealed chamber can allow the gas in the liquid in the pipeline to pass through when the sealed chamber is under negative pressure, so that the gas in the liquid in the pipeline is removed after passing through the sealed chamber; Optionally, the degassing device includes a negative pressure device and an exhaust pipeline, one end of the exhaust pipeline is connected to the closed chamber, and the negative pressure device is arranged on the exhaust pipeline and is used to generate negative pressure in the closed chamber.

6. The method according to claim 5, characterized in that The degassing device comprises a detector located between the negative pressure device and the closed chamber, the detector is connected to the exhaust pipeline, and the detector is used to detect the air pressure in the closed chamber through the exhaust pipeline; The determining whether the degassing device is normal includes: Using the detector to obtain the air pressure in the closed chamber; judging whether the degassing device is normal according to the change of the air pressure; Optionally, judging whether the degassing device is normal according to the change of the air pressure includes: Acquiring a first air pressure in the sealed chamber at an initial moment; Acquiring a second air pressure in the sealed chamber at a preset time interval; Calculating an air pressure change rate using the first air pressure, the second air pressure and the preset time interval; Comparing the air pressure change rate with a preset change rate to determine whether the degassing device is normal; Optionally, comparing the air pressure change rate with a preset change rate to determine whether the degassing device is normal includes: When the negative pressure device is working, if the air pressure change rate is greater than or equal to the preset change rate, the degassing device is judged to be normal; if the air pressure change rate is less than the preset change rate, the degassing device is judged to be abnormal; Optionally, comparing the air pressure change rate with a preset change rate to determine whether the degassing device is normal includes: When the negative pressure device stops working, if the air pressure change rate is less than or equal to the preset change rate, the degassing device is judged to be normal; if the air pressure change rate is greater than the preset change rate, the degassing device is judged to be abnormal; Optionally, judging whether the degassing device is normal according to the change of the air pressure includes: When the negative pressure device stops working, obtaining a first moment when the air pressure is a first threshold, and obtaining a second moment when the air pressure is a second threshold, wherein the second threshold is greater than the first threshold; Calculating a time difference using the first moment and the second moment; Compare the time difference with a preset time difference to determine whether the degassing device is normal; Optionally, comparing the time difference with a preset time difference to determine whether the degassing device is normal includes: If the time difference is greater than or equal to the preset time difference, it is determined that the degassing device is normal; if the time difference is less than the preset time difference, it is determined that the degassing device is abnormal; Optionally, judging whether the degassing device is normal according to the change of the air pressure includes: When the negative pressure device stops working, recording the air pressure as a first threshold; When the air pressure reaches a second threshold, starting the negative pressure device; If the air pressure is greater than the first threshold within a preset time, the degassing device is judged to be abnormal; if the air pressure is less than or equal to the first threshold within a preset time, the degassing device is judged to be normal, and the second threshold is greater than the first threshold; Optionally, judging whether the degassing device is normal according to the change of the air pressure includes: When the negative pressure device stops working, recording the air pressure as a first threshold; At a preset time interval, determining whether the air pressure in the sealed chamber is greater than the first threshold; If the air pressure in the sealed chamber is greater than the first threshold, obtaining a rate of change of the air pressure; If the rate of change of the air pressure is greater than a preset rate of change, the degassing device is judged to be abnormal; otherwise, the degassing device is judged to be normal.

7. The method according to claim 6, characterized in that The method further comprises: If the degassing device is normal, start the negative pressure device; If the degassing device is abnormal, the negative pressure device will not be started.

8. The method according to any one of claims 4 to 7, characterized in that: The reaction device comprises a liquid inlet and a liquid outlet; The pipeline includes a first pipeline and a second pipeline, the first pipeline is connected to the liquid inlet, the second pipeline is connected to the liquid outlet, the degassing device includes a first degassing device, and the first pipeline is connected to the first degassing device; When the degassing device is normal, controlling the liquid in the pipeline to pass through the degassing device for degassing includes: Controlling the liquid in the first pipeline to be degassed through the first degassing device and allowing the degassed liquid to enter the reaction device; Optionally, the degassing device includes a second degassing device, and the second pipeline is connected to the second degassing device; When the degassing device is normal, controlling the liquid in the pipeline to pass through the degassing device for degassing includes: Controlling the liquid flowing out of the reaction device and entering the second pipeline to be degassed through the second degassing device; Optionally, the degassing device comprises a third degassing device, The pipeline includes a bypass pipeline, one end of the bypass pipeline is connected to the liquid inlet, the other end of the bypass pipeline is connected to the liquid outlet, and the bypass pipeline is connected to the third degassing device; When the degassing device is normal, controlling the liquid in the pipeline to pass through the degassing device for degassing includes: Controlling the liquid flowing out of the reaction device to enter the bypass pipeline, and allowing the liquid in the bypass pipeline to enter the third degassing device for degassing; Optionally, the method further comprises: The degassed liquid in the bypass pipeline is allowed to enter the reaction device.

9. A sequencing system, characterized in that: A fluid system comprising any one of claims 1 to 3.

10. A computer storage medium, characterized in that: When the computer program is executed by a processor, the processor is caused to implement the method according to any one of claims 4 to 8.

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