Adsorption device applied to raw urine adsorption and urokinase extraction

Abstract

The invention discloses an adsorption device applied to raw urine adsorption and urokinase extraction, and relates to the technical field of adsorption equipment. The adsorption system is arranged outside the shell and is composed of an adsorption column and an adsorption liquid circulating device, the adsorption column comprises a rotating roller and a multi-layer silica gel adsorption medium attached to the surface of the rotating roller, and the adsorption liquid circulating device comprises a liquid storage tank, a first stainless steel centrifugal pump, a Teflon liquid inlet pipe and a Teflon liquid outlet pipe. Adsorption liquid stored in the liquid storage tank is circularly conveyed into the adsorption column through a stainless steel centrifugal pump I, so that a silica gel adsorption medium is in full contact with the adsorption liquid, adsorption enzyme in the adsorption liquid is absorbed, circular operation of the adsorption liquid and eluent is realized by utilizing the adsorption system and the separation system, and the surface of the adsorption column is washed through the flowing adsorption liquid and eluent; the depth of the adsorption liquid and the eluent entering the silica gel adsorption medium is further increased, the adsorption of urokinase in the adsorption liquid is improved, and the elution strength of the surface of the adsorption column is enhanced.

Description

Technical Field

[0001] The invention relates to the field of adsorption equipment, in particular to an adsorption device used for adsorbing raw urine and extracting urokinase. Background Art

[0002] Urokinase is a weakly alkaline substance, and weakly acidic cation exchange resin has a good adsorption effect on it. Urokinase extraction is now mostly done by collecting urine and then extracting it by stirring in a container. Urokinase is a serine protease produced by human renal tubular epithelial cells. It is an alkaline protein with an isoelectric point of about pH 8.7. It is a white non-crystalline powder that is easily soluble in water. The dilute solution is unstable and must be used fresh, and must not be diluted with an acidic solution. The freeze-dried state can be stable for several years. Urokinase is a highly specific proteolytic enzyme. Its activity on synthetic substrates is similar to that of trypsin and plasmin, and it also has the activity of esterase.

[0003] A Chinese patent with authorization announcement number CN219721991U discloses a urokinase adsorption column, comprising: a pipe arranged at the bottom of a pool body, the top of the pipe is connected to the pool cavity, a limit plate is installed in the middle of the pipe, a grid plate is arranged in the middle of the limit plate, a cylinder is arranged in the pipe, and a limit component is installed in the pipe for limiting and fixing the cylinder. During use, when the urokinase adsorption column needs to be replaced, the cylinder is lifted out of the pipe through the end head, and the threaded end head is rotated to take out the urokinase adsorption column in the accommodating cavity of the cylinder, and a new urokinase adsorption column is placed, and the urokinase adsorption column is sealed into the accommodating cavity through the threaded end head, and then the cylinder is inserted into the pipe, and the cylinder is limited and fixed under the action of the limit component. The replacement process is convenient, the replacement time is shortened, and the replacement efficiency is improved.

[0004] In actual extraction of urokinase, a small number of adsorption columns are often placed in a large amount of adsorption liquid so that the adsorption columns are completely soaked, thereby achieving extraction of urokinase, resulting in a large amount of urine waste and a large amount of urokinase loss; Secondly, in the method of extracting urokinase by infiltration of adsorption columns, if a large number of adsorption columns need to be placed, the volume of the adsorption columns occupies a certain position, and all the adsorption columns cannot be effectively used. Manual infiltration is often required, which is time-consuming and labor-intensive. In the process of adsorption of urine or urokinase, the traditional urokinase extraction method has problems such as complex operation, low efficiency, and easy pollution. Secondly, the cleanliness of the equipment for extracting urine is crucial to the extraction of urine or urokinase.

[0005] Therefore, the present invention proposes an adsorption device for adsorbing raw urine and extracting urokinase, aiming to improve the extraction efficiency and quality of urokinase and reduce production costs and environmental pollution. Summary of the invention

[0006] In view of the above problems in the prior art, the present invention is proposed.

[0007] In order to solve the above technical problems, the present invention provides the following technical solutions: an adsorption device for adsorbing raw urine and extracting urokinase, comprising a housing; The adsorption system is arranged inside and outside the shell, and is composed of an adsorption column and an adsorption liquid circulation device. The adsorption column includes a roller and a multi-layer silica gel adsorption medium attached to the surface of the roller. The adsorption liquid circulation device includes a liquid storage tank, a stainless steel centrifugal pump, a Teflon liquid inlet pipe and a Teflon liquid outlet pipe. The adsorption liquid stored in the liquid storage tank is circulated and transported to the adsorption column by the stainless steel centrifugal pump, so that the silica gel adsorption medium is fully in contact with the adsorption liquid to absorb the adsorption enzyme in the adsorption liquid. The separation system is arranged outside the housing and is composed of an eluent circulation device and a collecting bottle. The eluent circulation device comprises a stainless steel liquid outlet pipe, a stainless steel liquid inlet pipe and a stainless steel centrifugal pump 2. The eluent stored in the collecting bottle is circulated and transported to the adsorption column by the stainless steel centrifugal pump 2 to elute the urokinase on the multi-layer silica gel adsorption medium. The eluent enters the collecting bottle and is collected. A rotating device is arranged inside the shell, and includes a transmission component and two transmission belts. The number of the adsorption columns is multiple groups, and the multiple groups of adsorption columns are arranged at equal intervals along the motion trajectory of the transmission belt, and two groups of transmission gears are fixed at both ends of each group of adsorption columns. The transmission component drives the multiple groups of adsorption columns to operate along the motion trajectory of the transmission belt, and the transmission component drives the adsorption columns to rotate through the transmission gears.

[0008] As a preferred embodiment of the adsorption device for adsorbing raw urine and extracting urokinase according to the present invention, the outer shell is detachably arranged, and consists of a left shell and a right shell. No less than two groups of locks are installed on the side of the outer shell, and the left shell and the right shell are tightly connected by the locks.

[0009] As a preferred embodiment of the adsorption device for adsorbing raw urine and extracting urokinase according to the present invention, a group of external pipes are welded on the surfaces of the left shell and the right shell, and the transmission assembly includes two groups of mounting plates, which are symmetrically arranged about the vertical center line of the shell, and the mounting plates are used to position the transmission belt.

[0010] As a preferred embodiment of the adsorption device for adsorbing raw urine and extracting urokinase according to the present invention, the mounting plate comprises an outer mounting plate and an inner mounting plate, the transmission belt is engaged between the outer mounting plate and the inner mounting plate, a plurality of mounting holes for installing a roller are provided on the surface of the transmission belt, and a plurality of continuous triangular teeth are installed at the bottom end of the inner mounting plate.

[0011] As a preferred solution of the adsorption device for adsorbing raw urine and extracting urokinase as described in the present invention, two groups of transmission rollers are arranged inside the transmission belt, and each group of the transmission rollers has a plurality of transmission rollers, and the plurality of transmission rollers penetrate the mounting plate and rotate on the surface of the mounting plate, and the surface of the mounting plate is provided with through grooves for the transmission rollers to penetrate.

[0012] As a preferred solution of the adsorption device for adsorbing raw urine and extracting urokinase as described in the present invention, the installation sizes of the multiple transmission rollers are divided into large transmission rollers and small transmission rollers, the large transmission roller fits tightly with the transmission belt, and a ring gear is sleeved on one end of one of the transmission rollers that passes through the outer mounting plate, and a group of driving parts are installed on the surface of the outer mounting plate, and the driving parts drive the ring gear to rotate.

[0013] As a preferred solution of the adsorption device for adsorbing raw urine and extracting urokinase described in the present invention, one end of the large transmission roller passing through the outer mounting plate is sleeved with a transmission belt, and two groups of the transmission rollers form a synchronous transmission arrangement through the transmission belt.

[0014] As a preferred solution of the adsorption device for adsorbing raw urine and extracting urokinase described in the present invention, a plurality of connecting rods are installed through the interior of the mounting plate, and two elastic air bags are sleeved on both ends of the connecting rods that penetrate the mounting plate, and a group of intermediate connecting tubes are installed through the surface of the mounting plate.

[0015] As a preferred solution of the adsorption device for adsorbing raw urine and extracting urokinase according to the present invention, a group of inner sieve cylinders is provided inside the outer shell, and two groups of inner pipe connections are installed at both ends of the inner sieve cylinders. The two groups of inner pipe connections are matched with the middle connecting pipe, and the position of the inner sieve cylinder is fixed by docking the inner pipe connection and the middle connecting pipe.

[0016] As a preferred embodiment of the adsorption device for adsorbing raw urine and extracting urokinase according to the present invention, the inner sieve cylinder comprises a left inner shell and a right inner shell, the left inner shell and the right inner shell are plug-connected, and the inner sieve cylinder comprises side panels at both ends; two groups of side panels extend inwardly with two groups of frame frames, and a filter membrane is fixed between the frame frames and the side panels.

[0017] Beneficial effects of the present invention: The device utilizes an adsorption system and a separation system to realize the circulation of the adsorption liquid and the elution liquid. The adsorption column surface is flushed by the flowing adsorption liquid and the elution liquid, which further increases the depth of the adsorption liquid and the elution liquid into the silica gel adsorption medium, increases the adsorption of urokinase in the adsorption liquid, and enhances the elution strength on the adsorption column surface. The device drives multiple groups of adsorption columns to run along the motion track of the transmission belt by using a transmission component. The transmission component drives the adsorption column to rotate through the transmission gear, so that each adsorption column intermittently enters the adsorption liquid and the eluent, prolonging the infiltration time of the adsorption liquid and the eluent on the adsorption column. At the same time, the rotation of the adsorption column is used to increase the contact area between the adsorption column and the eluent or the adsorption liquid, and gravity is used to increase the penetration of the infiltrated adsorption liquid and the eluent on the adsorption column, thereby improving the adsorption efficiency and extraction efficiency of urokinase; The device can select different numbers of adsorption columns to be installed according to the actual amount of adsorption liquid. When the adsorption columns are inserted into the two sets of mounting plates, different numbers of adsorption columns can be selected and installed according to the amount of adsorption liquid to ensure the extraction of urokinase, with a wide range of applications and strong practicality. The device is provided with an inner sieve cylinder installed inside the outer shell, and the inner sieve cylinder is installed in the circulation of the adsorption system and the separation system, so that the running adsorption liquid and the running elution liquid are screened through the inner sieve cylinder in each cycle, thereby preventing broken cell fragments from entering the urokinase liquid and reducing the output quality of urokinase; The device modularizes the outer shell, mounting plate and inner sieve cylinder, and can realize detachable installation, which is convenient for later cleaning and maintenance. At the same time, the outer shell, mounting plate and inner sieve cylinder are tightly connected through the elastic force of the lock and the elastic airbag, thereby ensuring the sealing of the adsorption device. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the accompanying drawings required for use in the description of the embodiments will be briefly introduced below. Obviously, the accompanying drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other accompanying drawings can be obtained based on these accompanying drawings without paying creative work.

[0019] Figure 1 It is a schematic diagram of the structure of the adsorption device used for adsorbing raw urine and extracting urokinase in the present invention; Figure 2 It is a partial structural diagram of the adsorption device used for adsorbing raw urine and extracting urokinase in the present invention; Figure 3 For the present invention Figure 2 A magnified view of the structure of part A; Figure 4 It is a schematic diagram of the overall structure of the mounting plate in the present invention; Figure 5 It is a schematic diagram of the structure of the transmission component part in the present invention; Figure 6 It is an axonometric diagram of the overall structure of the inner screening cylinder in the present invention; Figure 7It is a schematic diagram of the overall structure of the inner screening cylinder in the present invention; Figure 8 It is a partial structural plan view of the adsorption device used for adsorbing raw urine and extracting urokinase in the present invention; Fig. 9 For the present invention Figure 8 Enlarged view of the structure of part B.

[0020] Reference numerals: 1, liquid storage tank; 2, stainless steel centrifugal pump 1; 3, Teflon liquid inlet pipe; 4, Teflon liquid outlet pipe; 5, housing; 501, outer mounting plate; 502, transmission belt; 503, inner mounting plate; 504, limit ring; 505, mounting hole; 506, through groove; 507, silica gel adsorption medium; 508, roller; 509, transmission gear; 510, inner sieve cylinder; 511, left housing; 512, right housing; 513, right housing; 514, left housing; 515, right housing; 516, right housing; 517, right housing; 518, left housing; 519, right housing; 520, right housing; 521, right housing; 522, right housing; 523, right housing; 524, right housing; 525, right housing; 526, right housing; 527, right housing; 528, right housing; 529, right housing; 530, right housing; 531, right housing; 532, right housing; 533, right housing; 534, right housing; 535, right housing; 536, right housing; 537, right housing; 538, right housing; 539, right housing; 540, right housing; 541, right housing; 542, right housing; 543, right housing; 544, right housing; 545, right housing; 546, right housing; 547, right housing; 548, right housing; 549, right housing; 550, right housing; 551, right housing; 552, right housing; 553, right housing; 554, right housing; 555, right housing; 556, right housing; 557 3. Locking piece; 514. L-shaped plate; 515. Fastening mechanism; 516. Intermediate connecting pipe; 517. Elastic airbag; 518. Transmission roller; 519. Connecting rod; 520. Transmission belt; 521. Driving piece; 522. Ring gear; 523. Filter membrane; 524. Frame; 525. Internal pipe; 526. Sealing leather ring; 6. Stainless steel liquid outlet pipe; 7. Collecting bottle; 8. Stainless steel liquid inlet pipe; 9. Stainless steel centrifugal pump II. DETAILED DESCRIPTION

[0021] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the specific implementation methods of the present invention are described in detail below in conjunction with the accompanying drawings.

[0022] In the following description, many specific details are set forth to facilitate a full understanding of the present invention, but the present invention may also be implemented in other ways different from those described herein, and those skilled in the art may make similar generalizations without violating the connotation of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0023] Secondly, the term "one embodiment" or "embodiment" as used herein refers to a specific feature, structure, or characteristic that may be included in at least one implementation of the present invention. The term "in one embodiment" that appears in different places in this specification does not necessarily refer to the same embodiment, nor is it a separate or selective embodiment that is mutually exclusive with other embodiments.

[0024] Example 1

[0025] Reference Figure 1 , Figure 2 As shown, it is the first embodiment of the present invention, which provides an adsorption device for adsorbing raw urine and extracting urokinase, comprising: The housing 5 is detachably arranged. This design not only facilitates the installation and maintenance of the internal equipment components, but also greatly improves the cleaning efficiency. It is composed of a left housing 511 and a right housing 512. No less than two groups of locks 513 are installed on the side of the housing 5. No less than two groups of connecting locks 513 are symmetrically distributed on both sides. The housing 5 is tightly connected through a unique ball rod and a buckling mechanism 515. This design not only enhances the stability of the structure, but also simplifies the disassembly and assembly process and improves the convenience of operation. The two groups of locks 513 are symmetrically distributed on both sides of the housing 5. The left housing 5 is connected through the locks 513. The left shell body 511 and the right shell body 512 are tightly connected, the locking member 513 includes two groups of L-shaped plates 514 for fixing the outer walls of the left shell body 511 and the right shell body 512, a group of clubs are fixed on the surface of the L-shaped plates 514, and a group of buckling mechanisms 515 are rotatably installed on the surface of the clubs on the left shell body 511. The buckling mechanism 515 is rotatably buckled on the surface of the clubs on the right shell body 512. The buckling mechanism 515 includes a ring sleeved on the surface of the club, a telescopic rod and a buckle claw. The ring and the buckle claw are respectively fixed to the two ends of the telescopic rod, and the tight connection between the left shell body 511 and the right shell body 512 is achieved through the locking member 513.

[0026] The adsorption system is arranged inside the housing 5, and is composed of an adsorption column and an adsorption liquid circulation device. The adsorption column includes a roller 508 and a multi-layer silica gel adsorption medium 507 attached to the surface of the roller 508. The roller 508 is made of high-quality stainless steel material to ensure the corrosion resistance of the equipment. The silica gel adsorption medium 507 uses special silica gel with high specific surface area and high adsorption performance, which can achieve efficient adsorption of urokinase in raw urine. The adsorption liquid circulation device includes a liquid storage tank 1, a stainless steel centrifugal pump 2, a Teflon liquid inlet pipe 3 and a Teflon liquid outlet pipe 4. The Teflon liquid inlet pipe 3 is used to connect the liquid storage tank 1 and the stainless steel centrifugal pump 2. The centrifugal pump 2, the stainless steel centrifugal pump 2 and the housing 5 realize the circulation of the adsorption liquid stored in the liquid storage tank 1 to the adsorption column in the housing 5 through the stainless steel centrifugal pump 2, so that the silica gel adsorption medium 507 is fully in contact with the adsorption liquid to absorb the adsorption enzyme in the adsorption liquid. The Teflon liquid outlet pipe 4 is used to connect the housing 5 and the liquid storage tank 1. The liquid storage tank 1, the stainless steel centrifugal pump 2, the Teflon liquid inlet pipe 3, the Teflon liquid outlet pipe 4 and the housing 5 form a closed loop to realize the circulation of the adsorption liquid. Through the flow of the adsorption liquid, the contact area between the adsorption liquid and the adsorption column is increased, and the adsorption efficiency of urokinase in the adsorption liquid is improved. The separation system is arranged outside the housing 5, and is composed of an eluent circulation device and a collecting bottle 7. The eluent circulation device comprises a stainless steel liquid outlet pipe 6, a stainless steel liquid inlet pipe 8 and a stainless steel centrifugal pump 9. The stainless steel liquid inlet pipe 8 is used to connect the collecting bottle 7 and the stainless steel centrifugal pump 9, and the stainless steel centrifugal pump 9 and the housing 5. The eluent stored in the collecting bottle 7 is circulated and transported to the adsorption column by the stainless steel centrifugal pump 9 to elute the urokinase on the multi-layer silica gel adsorption medium 507. The stainless steel liquid outlet pipe 6 is used to connect the housing 5 and the collecting bottle 7. The eluent enters the collecting bottle 7 and is collected, and then is dried by a siphon drying device to achieve the extraction of crude urokinase. This design improves the elution efficiency and reduces the waste of the eluent. The device adopts a highly efficient silica gel adsorption medium 507 and a separation system, which can achieve highly efficient adsorption of urokinase in raw urine and improve the extraction efficiency of urokinase.

[0027] Example 2

[0028] Reference Figure 3 to Figure 9 As shown, it is a second embodiment of the present invention. This embodiment is based on the previous embodiment, and the difference is that the adsorption device used for adsorption of raw urine and extraction of urokinase also includes: a rotating device, which is arranged inside the shell 5, and includes a transmission component and two transmission belts 502. The number of adsorption columns is multiple groups, and the multiple groups of adsorption columns are arranged at equal intervals along the movement trajectory of the transmission belt 502, and two groups of transmission gears 509 are fixed at both ends of each group of adsorption columns. The transmission component drives the multiple groups of adsorption columns to operate along the movement trajectory of the transmission belt 502. The transmission component drives the adsorption columns to rotate through the transmission gears 509. The device is provided with multiple groups of adsorption columns, and the multiple groups of adsorption columns are driven by the transmission component, so that the multiple groups of adsorption columns operate along the movement trajectory of the transmission belt 502. When running to the bottom of the shell 5, they are immersed in the adsorption liquid to achieve adsorption of urokinase in the adsorption liquid. At the same time, the adsorption columns rotate by themselves through the transmission component, so that the adsorption columns are fully immersed in the adsorption liquid to achieve full adsorption of the adsorption columns.

[0029] Specifically, a group of external pipes are welded on the surface of the left shell 511 and the right shell 512. The external pipe on the left shell 511 is connected to the Teflon liquid inlet pipe 3. At the same time, a sealing leather ring 526 is sleeved at the connection between the external pipe and the Teflon liquid inlet pipe 3 to tighten the connection between the external pipe and the Teflon liquid inlet pipe 3 to prevent liquid leakage at the connection between the external pipe and the Teflon liquid inlet pipe 3. The transmission assembly includes two groups of mounting plates, which are symmetrically arranged about the vertical center line of the outer shell 5. The mounting plates are used to position the transmission belt 502, and the mounting plates are used to fix the shape of the transmission belt 502 to ensure that the movement trajectory of the transmission belt 502 is shaped like a waist-shaped hole. At the same time, the mounting plates fix the position of the transmission belt 502, that is, the positions of multiple groups of adsorption columns are simultaneously limited.

[0030] Specifically, the mounting plate includes an outer mounting plate 501 and an inner mounting plate 503, and the transmission belt 502 is clamped between the outer mounting plate 501 and the inner mounting plate 503. A plurality of mounting holes 505 for installing rollers 508 are provided on the surface of the transmission belt 502, and a plurality of continuous triangular teeth are installed at the bottom end of the inner mounting plate 503; two groups of transmission rollers 518 are provided inside the transmission belt 502, and each group of transmission rollers 518 has a plurality of transmission rollers, and a plurality of transmission rollers 518 pass through the mounting plate, and limit rings 504 are threadedly connected at both ends of the transmission rollers 518 passing through the mounting plate to limit the position of the transmission rollers 518, and the transmission rollers 518 rotate on the surface of the mounting plate, and a through groove 506 for the transmission rollers 518 to pass through is provided on the surface of the mounting plate.

[0031] Specifically, the installation sizes of multiple transmission rollers 518 are divided into large transmission rollers and small transmission rollers. The large transmission roller fits tightly with the transmission belt 502. One end of a transmission roller 518 that passes through the outer mounting plate 501 is sleeved with a ring gear 522, and a group of driving members 521 are installed on the surface of the outer mounting plate 501. The driving member 521 drives the ring gear 522 to rotate. The ring gear 522 is sleeved on a large transmission roller. The driving member 521 includes a motor clamped between the outer mounting plate 501 and the transmission belt 502 and a driving member 521 fixed to the end of the motor output shaft. The driving member 521 drives the ring gear 522 to rotate, so that the large transmission roller fixed with the ring gear 522 is transmitted, and the large transmission roller transmits the next transmission roller through the small transmission roller fitted therewith. A transmission connection is formed between the multiple large transmission rollers and the multiple small transmission rollers, and the transmission directions of the multiple large transmission rollers are consistent through the small transmission rollers.

[0032] Example 3

[0033] This is the third embodiment of the present invention. This embodiment is based on the previous embodiment, except that Figures 4 to 9 Specifically, one end of the large transmission roller passing through the outer mounting plate 501 is sleeved with a transmission belt 520, and two sets of transmission rollers 518 form a synchronous transmission arrangement through the transmission belt 520. Specifically, a plurality of connecting rods 519 are installed through the inside of the mounting plate, and the outer mounting plate 501 and the transmission belt 502 are tightly connected through the connecting rods 519, and two elastic air bags 517 are sleeved at both ends of the connecting rods 519 passing through the mounting plate, and a group of intermediate connecting tubes 516 are installed through the surface of the mounting plate.

[0034] A set of inner screening cylinders 510 is disposed inside the housing 5, and two sets of inner pipes 525 are installed at both ends of the inner screening cylinder 510; The two groups of internal pipes 525 are matched with the middle connecting pipe 516, and the position of the inner screening cylinder 510 is fixed by docking the internal pipes 525 and the middle connecting pipe 516. The multiple elastic air bags 517 located on both sides of the outer mounting plate 501 and the inner mounting plate 503 are deformed by pressure to generate a reaction force to determine the position of the outer shell 5, the mounting plate and the inner screening cylinder 510. Among them, the outer shell 5, the mounting plate and the inner screening cylinder 510 are modularly processed and can be detachably installed to facilitate subsequent cleaning and maintenance. At the same time, the elastic force of the locking piece 513 and the elastic air bag 517 is used to achieve a tight connection between the outer shell 5, the mounting plate and the inner screening cylinder 510, thereby ensuring the sealing of the adsorption device.

[0035] Specifically, the inner sieve cylinder 510 includes a left inner shell and a right inner shell, and the left inner shell and the right inner shell are plug-connected. The inner sieve cylinder 510 includes side panels at both ends; two groups of side panels extend inward with two groups of frame frames 524, and a filter membrane 523 is fixed between the frame frames 524 and the side panels. The detachably connected inner sieve cylinder 510 is convenient for timely cleaning to ensure the efficiency of screening. The inner sieve cylinder 510 is used to rotationally screen the adsorption liquid and the elution liquid to ensure the quality of the eluted urokinase.

[0036] Working principle: An ultrasonic water level meter is installed inside the shell 5, which monitors the water level inside the shell 5 and sends a signal. The stainless steel centrifugal pump 1 2 or the stainless steel centrifugal pump 2 9 receives the signal and controls the water level inside the shell 5 to ensure that the water level inside the shell 5 is higher than the low-level adsorption column, so that the adsorption column is immersed in the liquid; According to the actual amount of adsorption liquid, different numbers of adsorption columns are selected for installation. When inserting the adsorption columns in the two sets of mounting plates, it is necessary to ensure that when the adsorption columns are running along the track of the transmission belt 502, the transmission gears 509 at both ends of the roller 508 in the adsorption column are meshed with the continuous triangle ruler at the bottom end of the inner mounting plate 503, so that the adsorption column can rotate by itself, so that the adsorption column rotates in the liquid, the contact area between the adsorption column and the adsorption liquid is increased, and the adsorption efficiency of the adsorption liquid is increased. The device can choose to install different numbers of adsorption columns according to the amount of adsorption liquid to ensure the extraction of urokinase, and has a wide range of applications and strong practicality. The device utilizes an adsorption system and a separation system to realize the circulation of the adsorption liquid and the elution liquid. The adsorption column surface is flushed by the flowing adsorption liquid and the elution liquid, and the depth of the adsorption liquid and the elution liquid entering the silica gel adsorption medium 507 is further increased, the adsorption of urokinase in the adsorption liquid is increased, and the elution strength on the adsorption column surface is enhanced. The device drives multiple groups of adsorption columns to run along the motion track of the transmission belt 502 by using a transmission component. The transmission component drives the adsorption columns to rotate through the transmission gear 509, so that each adsorption column intermittently enters the adsorption liquid and the eluent, prolongs the action time of the single infiltration adsorption liquid and the eluent on the adsorption column, utilizes the rotation action of the adsorption column to increase the contact area between the adsorption column and the eluent or the adsorption liquid, utilizes gravity to increase the penetration of the infiltration adsorption liquid and the eluent on the adsorption column, and improves the adsorption efficiency and extraction efficiency of urokinase; The device is provided with an inner sieve cylinder 510 installed inside the housing 5, and the inner sieve cylinder 510 is installed in the circulation of the adsorption system and the separation system, so that the running adsorption liquid and the running elution liquid are screened through the inner sieve cylinder 510 in each cycle, thereby preventing broken cell fragments from entering the urokinase liquid and reducing the output quality of urokinase; The device modularizes the outer shell 5, the mounting plate and the inner sieve cylinder 510, and can realize detachable installation, which is convenient for later cleaning and maintenance. At the same time, through the elastic force of the lock 513 and the elastic airbag 517, the outer shell 5, the mounting plate and the inner sieve cylinder 510 are tightly connected, ensuring the sealing of the adsorption device.

[0037] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the spirit and scope of the technical solutions of the present invention, which should all be included in the scope of the claims of the present invention.

Claims

1. An adsorption device for adsorbing raw urine and extracting urokinase, characterized in that: include: Shell (5); An adsorption system is arranged inside the housing (5), and is composed of an adsorption column and an adsorption liquid circulation device. The adsorption column comprises a rotating roller (508) and a multi-layer silica gel adsorption medium (507) attached to the surface of the rotating roller (508). The adsorption liquid circulation device comprises a liquid storage tank (1), a stainless steel centrifugal pump (2), a Teflon liquid inlet pipe (3) and a Teflon liquid outlet pipe (4). The adsorption liquid stored in the liquid storage tank (1) is circulated and transported to the adsorption column through the stainless steel centrifugal pump (2), so that the silica gel adsorption medium (507) is fully in contact with the adsorption liquid to absorb the adsorption enzyme in the adsorption liquid. A separation system is arranged outside the housing (5), and is composed of an eluent circulation device and a collection bottle (7). The eluent circulation device comprises a stainless steel liquid outlet pipe (6), a stainless steel liquid inlet pipe (8) and a second stainless steel centrifugal pump (9). The eluent stored in the collection bottle (7) is circulated and transported to the adsorption column through the second stainless steel centrifugal pump (9) to elute the urokinase on the multi-layer silica gel adsorption medium (507). The eluent enters the collection bottle (7) and is collected; A rotating device is arranged inside the housing (5), and comprises a transmission component and two transmission belts (502). The number of the adsorption columns is multiple groups, and the multiple groups of adsorption columns are arranged at equal intervals along the movement trajectory of the transmission belt (502), and two groups of transmission gears (509) are fixed at both ends of each group of adsorption columns. The transmission component drives the multiple groups of adsorption columns to operate along the movement trajectory of the transmission belt (502), and the transmission component drives the adsorption columns to rotate via the transmission gears (509).

2. The adsorption device for adsorbing raw urine and extracting urokinase according to claim 1, characterized in that: The housing (5) is detachably arranged and consists of a left housing (511) and a right housing (512). No less than two groups of locking members (513) are installed on the side of the housing (5), and the left housing (511) and the right housing (512) are tightly connected via the locking members (513).

3. The adsorption device for adsorbing raw urine and extracting urokinase according to claim 2, characterized in that: A group of external pipes are welded on the surfaces of the left shell (511) and the right shell (512), and the transmission assembly includes two groups of mounting plates, which are symmetrically arranged about the vertical center line of the shell (5), and the mounting plates are used to position the transmission belt (502).

4. The adsorption device for adsorbing raw urine and extracting urokinase according to claim 3, characterized in that: The mounting plate comprises an outer mounting plate (501) and an inner mounting plate (503); the transmission belt (502) is engaged between the outer mounting plate (501) and the inner mounting plate (503); a plurality of mounting holes (505) for mounting a roller (508) are provided on the surface of the transmission belt (502); and a plurality of continuous triangular teeth are mounted at the bottom end of the inner mounting plate (503).

5. The adsorption device for adsorbing raw urine and extracting urokinase according to claim 4, characterized in that: Two groups of transmission rollers (518) are arranged inside the transmission belt (502), and each group of the transmission rollers (518) has a plurality of transmission rollers (518). The plurality of transmission rollers (518) penetrate the mounting plate and rotate on the surface of the mounting plate. The surface of the mounting plate is provided with a through groove (506) for the transmission rollers (518) to penetrate.

6. The adsorption device for adsorbing raw urine and extracting urokinase according to claim 5, characterized in that: The plurality of transmission rollers (518) are divided into large transmission rollers and small transmission rollers according to their installation sizes. The large transmission roller fits tightly with the transmission belt (502). A ring gear (522) is sleeved on one end of one of the transmission rollers (518) that passes through the outer mounting plate (501). A group of driving members (521) are mounted on the surface of the outer mounting plate (501). The driving members (521) drive the ring gear (522) to rotate.

7. The adsorption device for adsorbing raw urine and extracting urokinase according to claim 6, characterized in that: One end of the large transmission roller that passes through the outer mounting plate (501) is sleeved with a transmission belt (520), and the two groups of transmission rollers (518) form a synchronous transmission arrangement through the transmission belt (520).

8. The adsorption device for adsorbing raw urine and extracting urokinase according to claim 7, characterized in that: A plurality of connecting rods (519) are installed through the interior of the mounting plate, and the connecting rods (519) penetrate the mounting plate and are sleeved with two elastic air bags (517) at both ends. A group of intermediate connecting pipes (516) are installed through the surface of the mounting plate.

9. The adsorption device for adsorbing raw urine and extracting urokinase according to claim 8, characterized in that: A group of inner sieve cylinders (510) is provided inside the outer shell (5), and two groups of inner pipes (525) are installed at both ends of the inner sieve cylinder (510). The two groups of inner pipes (525) are compatible with the middle connecting pipe (516), and the position of the inner sieve cylinder (510) is fixed by docking the inner pipes (525) and the middle connecting pipe (516).

10. The adsorption device for adsorbing raw urine and extracting urokinase according to claim 9, characterized in that: The inner sieve cylinder (510) comprises a left inner shell and a right inner shell, the left inner shell and the right inner shell are plug-connected, and the inner sieve cylinder (510) comprises side panels located at both ends; two groups of frame frames (524) extend inwardly from the two groups of side panels, and a filter membrane (523) is fixed between the frame frames (524) and the side panels.

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