Immune reconstruction compound for improving immune function and preventing and adjunctively treating tumors as well as preparation method and application of immune reconstruction compound

Abstract

The invention discloses an immune reconstruction compound for improving the immune function and preventing and assisting in treating tumors as well as a preparation method and application of the immune reconstruction compound, and relates to the technical field of preparation of related preparations for improving immunity. Comprising 180-220 parts of selenium-enriched garlic powder, 15-25 parts of grifola frondosa extract, 7-13 parts of lucid ganoderma extract, 9-12 parts of cordyceps militaris extract, 14-24 parts of dandelion extract, 17-25 parts of broccoli extract and 8-14 parts of turmeric. The selenium-rich garlic powder is used as a main component, the selenium-rich garlic powder is rich in selenium element, the effects of resisting tumors and improving immunity can be achieved, meanwhile, multiple components including the grifola frondosa extract, the ganoderma lucidum extract, the cordyceps militaris extract, the dandelion extract and the broccoli extract are used as auxiliary materials, and the effects of resisting tumors or preventing tumors are jointly achieved. Meanwhile, the inventor accidentally finds that the selenium-enriched garlic powder, the grifola frondosa extract and the cordyceps militaris extract have a synergistic effect in the experiment process, and the effects of resisting tumors, diminishing inflammation and improving immunity can be remarkably improved by compounding the selenium-enriched garlic powder, the grifola frondosa extract and the cordyceps militaris extract.

Description

Technical Field

[0001] The present invention belongs to the technical field of preparation of preparations related to improving immunity, and specifically relates to an immune reconstruction compound for improving immune function, preventing and assisting in the treatment of tumors, and a preparation method and application thereof. Background Art

[0002] Tumors are major diseases that threaten human health. Traditional surgery, radiotherapy, chemotherapy and other treatments have inhibited the progression of tumors to a certain extent, but it is difficult to control the recurrence and metastasis of tumors. With the continuous emergence of exciting clinical research results, anti-tumor immunotherapy has brought hope to cancer patients. The immune system has the ability to identify and eliminate foreign bodies. During the occurrence and development of tumors, tumors not only inhibit the innate immune response of the immune system through various pathways, but also "paralyze" infiltrating immune effector cells by limiting the "display" of their own "new" antigens and activating immune checkpoints, thereby achieving the purpose of escaping immune recognition and immune clearance. How to effectively activate anti-tumor immune responses, eliminate immune escape, and thus achieve the effect of immune clearance has always been the goal pursued in the field of tumor immunotherapy.

[0003] At present, there are endless medicinal and edible preparations on the market. Many medicinal and edible foods have the effects of activating the immune system, fighting tumors, and eliminating inflammatory factors. Therefore, it is very common to prepare medicinal and edible foods into health products with specific functions. However, the current health products that improve the function of the immune system are weak in efficacy, or the effects between the formulas are unclear, and they cannot play a good role in regulating the function of the immune system. In particular, the immune system of patients with malignant tumors is severely disturbed and damaged after surgery, and the effect of rebuilding the immune system after malignant tumor surgery is not ideal.

[0004] Therefore, how to improve the effect of the immune system reconstruction formula is a technical problem that technical personnel in this field urgently need to solve. Summary of the invention

[0005] In view of this, the present invention is proposed.

[0006] One of the purposes of the present invention is to provide an immune reconstitution compound.

[0007] The second object of the present invention is to provide a method for preparing the above compound.

[0008] The third object of the present invention is to provide the use of the above-mentioned compound.

[0009] On the one hand, an embodiment of the present invention provides an immune reconstruction compound for improving immune function, preventing and assisting in the treatment of tumors, comprising the following components in parts by weight: 180-220 parts of selenium-enriched garlic powder, 15-25 parts of Grifola frondosa extract, 7-13 parts of Ganoderma lucidum extract, 9-12 parts of Cordyceps militaris extract, 14-24 parts of Dandelion extract, 17-25 parts of Broccoli extract and 8-14 parts of Curcuma longa.

[0010] In a preferred embodiment, the following components are included in optimal weight proportions: 200 parts of selenium-enriched garlic powder, 20 parts of Grifola frondosa extract, 10 parts of Ganoderma lucidum extract, 10 parts of Cordyceps militaris extract, 20 parts of dandelion extract, 20 parts of broccoli extract and 10 parts of turmeric.

[0011] The second aspect of the embodiment of the present invention provides a method for preparing the above-mentioned compound, the process comprising:

[0012] S1. Weighing: weigh the raw materials according to a specific weight ratio;

[0013] S2, ultrafinely grinding the turmeric to 600-800 mesh, sieving, and obtaining turmeric powder;

[0014] S3, mixing and evenly stirring the Grifola frondosa extract, Ganoderma lucidum extract, Cordyceps militaris extract, Taraxacum officinale extract, and Broccoli extract to obtain a mixture 1;

[0015] S4. Add turmeric powder to mixture 1, stir evenly, freeze-dry and pulverize to obtain a compound powder.

[0016] In a preferred embodiment, in S4, freeze drying is freeze drying at -30 to -40°C for 1 to 2 hours.

[0017] In a preferred embodiment, in S4, the pulverization is ultrafine pulverization to 600-800 mesh.

[0018] The third aspect of the embodiments of the present invention provides the use of the compound prepared by the above method in the preparation of health products for auxiliary prevention or auxiliary treatment of anti-tumor.

[0019] The fourth aspect of the embodiments of the present invention provides the use of the compound prepared by the above method in the preparation of health products that assist in improving immunity and assist in eliminating anti-inflammatory and anti-inflammatory effects.

[0020] It can be known from the above technical scheme that the present invention uses selenium-enriched garlic powder as the main component, which is rich in selenium and can achieve the effect of anti-tumor and immunity enhancement. At the same time, the present invention is supplemented with a variety of components, such as maitake mushroom extract, ganoderma lucidum extract, cordyceps militaris extract, dandelion extract, and broccoli extract as auxiliary materials, which jointly play the effect of anti-tumor or tumor prevention. At the same time, the inventor accidentally discovered during the experiment that selenium-enriched garlic powder, maitake mushroom extract, and cordyceps militaris extract have a synergistic effect, and the compounding of the three can significantly improve the effects of anti-tumor, anti-inflammatory, and immunity enhancement. DETAILED DESCRIPTION

[0021] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0022] In the embodiments of the present invention, the effects of each component are as follows:

[0023] Selenium-enriched garlic powder: Selenium-enriched garlic contains a wealth of nutrients, the most notable of which is its high content of selenium. Selenium is an important trace element that plays an important role in human health. In addition to selenium, selenium-enriched garlic is also rich in vitamin C, vitamin B6, zinc, manganese and sulfide. The combined effect of these nutrients makes selenium-enriched garlic a food with multiple health benefits. The antioxidant effect of selenium-enriched garlic is one of its most important nutritional values. Selenium is an important antioxidant in the human body that can neutralize free radicals and reduce oxidative damage to cells and tissues. Excessive accumulation of free radicals is closely related to the occurrence and development of many diseases, including cardiovascular disease, cancer and neurodegenerative diseases. By eating selenium-enriched garlic, you can increase the intake of selenium in the body, improve antioxidant capacity, and reduce the damage of oxidative stress to the body. At the same time, selenium-enriched garlic is of great significance to the regulation of the immune system. Selenium is necessary for the normal function of the immune system and can enhance the activity of immune cells and the ability of immune response. The health of the immune system is crucial to the prevention and resistance of diseases. Eating selenium-enriched garlic can provide enough selenium to promote the normal functioning of the immune system, enhance the body's resistance, and reduce the risk of infection and disease. Selenium can inhibit the growth and spread of cancer cells through various mechanisms and promote apoptosis of cancer cells. In addition, sulfides and other active ingredients in selenium-rich garlic also have anti-cancer potential. Studies have shown that eating selenium-rich garlic can reduce the risk of certain cancers, including gastric cancer, colorectal cancer, and breast cancer.

[0024] Grifola frondosa extract: The polysaccharide components in Grifola frondosa extract can stimulate the activity of T-cells, NK cells and macrophages, thereby strengthening the function of the immune system and activating immune responses such as macrophages, natural killer cells, T-lymphocytes, as well as lymphokines and interleukins, which make it useful for the treatment of cancer. Studies have shown that it can reduce the side effects of chemotherapy and can induce apoptosis of cancer cells.

[0025] Ganoderma lucidum extract: Ganoderma lucidum extract is extracted by Ganoderma lucidum fermentation process. Its main ingredients are Ganoderma lucidum triterpenoids and Ganoderma lucidum polysaccharides, which have anti-cancer activity, can improve the function of the immune system, anti-oxidation, and eliminate inflammatory factors.

[0026] Cordyceps militaris extract: has antioxidant and anti-aging effects. According to traditional Chinese medicine theory, it can effectively replenish kidney yang, thus better promoting blood circulation throughout the body, and playing a role in improving immunity and anti-tumor.

[0027] Dandelion Extract: Dandelion extract has been used to treat liver and gallbladder obstruction, improve liver function, promote bile secretion and act as a diuretic.

[0028] Broccoli extract: The main component of broccoli extract is sulforaphane, which is a powerful anti-cancer substance. It can increase the activity of carcinogen detoxification enzymes and help cancer cells repair into normal cells. It is especially effective in preventing and treating gastric cancer and breast cancer. It not only supplements the vitamin C and selenium needed by the human body, but also provides rich carotene, which prevents the formation of precancerous cells and inhibits the growth of tumors. At the same time, broccoli extract also has the effects of anti-aging, improving immunity, and clearing bacteria from the lungs.

[0029] Turmeric: Its main ingredient is curcumin, which has the effects of anti-tumor, lowering blood lipids, and promoting blood circulation and removing blood stasis.

[0030] Example 1

[0031] An immune reconstruction compound comprises the following components in parts by weight: 180 parts of selenium-enriched garlic powder, 15 parts of maitake mushroom extract, 7 parts of ganoderma lucidum extract, 9 parts of cordyceps militaris extract, 14 parts of dandelion extract, 17 parts of broccoli extract and 8 parts of turmeric.

[0032] The preparation method of the above compound comprises:

[0033] S1. Weighing: weigh the raw materials according to a specific weight ratio;

[0034] S2, ultrafinely grinding the turmeric to 600 mesh, sieving, and obtaining turmeric powder;

[0035] S3, mixing and evenly stirring the Grifola frondosa extract, Ganoderma lucidum extract, Cordyceps militaris extract, Taraxacum officinale extract, and Broccoli extract to obtain a mixture 1;

[0036] S4. Add turmeric powder to mixture 1, stir evenly, freeze-dry at -30 to -40°C for 1 hour, and ultrafinely grind to 600 mesh to obtain a compound powder.

[0037] Example 2

[0038] An immune reconstruction compound comprises the following components in optimal weight portions: 200 parts of selenium-enriched garlic powder, 20 parts of maitake mushroom extract, 10 parts of ganoderma lucidum extract, 10 parts of cordyceps militaris extract, 20 parts of dandelion extract, 20 parts of broccoli extract and 10 parts of turmeric.

[0039] The preparation method of the above compound comprises:

[0040] S1. Weighing: weigh the raw materials according to a specific weight ratio;

[0041] S2, ultrafinely grinding the turmeric to 700 mesh, sieving, and obtaining turmeric powder;

[0042] S3, mixing and evenly stirring the Grifola frondosa extract, Ganoderma lucidum extract, Cordyceps militaris extract, Taraxacum officinale extract, and Broccoli extract to obtain a mixture 1;

[0043] S4. Add turmeric powder to mixture 1, freeze-dry at -30 to -40°C for 1.5 h, and ultrafinely grind to 700 mesh to obtain a compound powder.

[0044] Example 3

[0045] An immune reconstruction compound comprises the following components in parts by weight: 220 parts of selenium-enriched garlic powder, 25 parts of maitake mushroom extract, 13 parts of ganoderma lucidum extract, 12 parts of cordyceps militaris extract, 24 parts of dandelion extract, 25 parts of broccoli extract and 14 parts of turmeric.

[0046] The preparation method of the above compound comprises:

[0047] S1. Weighing: weigh the raw materials according to a specific weight ratio;

[0048] S2, ultrafinely grinding the turmeric to 800 mesh, sieving, and obtaining turmeric powder;

[0049] S3, mixing and evenly stirring the Grifola frondosa extract, Ganoderma lucidum extract, Cordyceps militaris extract, Taraxacum officinale extract, and Broccoli extract to obtain a mixture 1;

[0050] S4. Add turmeric powder to mixture 1, stir evenly, freeze-dry at -30 to -40°C for 2 h, and ultrafinely grind to 800 mesh to obtain a compound powder.

[0051] Example 4 Safety Evaluation

[0052] 20 SPF rats were selected, weighing 200±20g, with good activity, normal vital signs, and normal feeding. The 20 rats were fed with the compound preparation in Examples 1-3 at a daily feeding amount of 50g / kg for 2 consecutive months, and the vital signs of the rats were observed. The results showed that the vital signs were normal, and the structure and morphology of the kidney and spleen of the rats were normal. Therefore, the compound in Examples 1-3 had no toxic side effects.

[0053] Example 5 Antitumor Experiment

[0054] A549 non-small cell lung cancer cell line, SGC-7901 human gastric cancer cell line and 7721 human liver cancer cell line were selected, and Example 2 was dissolved in serum-containing DMEM complete medium to prepare different concentrations of drugs, the concentrations were: 0.02 mg / ml, 0.2 mg / ml, 2 mg / ml, and the experimental groups were as follows:

[0055] Positive control: tumor cells were treated with methotrexate at a dose of 2.5-3 ml / kg;

[0056] Experimental group 1: tumor cells were treated with the compound at a concentration of 0.02 mg / ml;

[0057] Experimental group 2: tumor cells were treated with the compound at a concentration of 0.2 mg / ml;

[0058] Experimental group 3: tumor cells were treated with the compound at a concentration of 2 mg / ml;

[0059] Negative control: tumor cells were treated with 2 ml of normal saline.

[0060] The cell survival rate was statistically analyzed, see Table 1;

[0061] Table 1 Tumor cell survival rate

[0062]

[0063] It can be seen from Table 1 that the compound of Example 2 of the present invention can significantly achieve the effect of anti-tumor and inhibiting tumor growth and proliferation.

[0064] Example 6 Verification of the synergistic effect of each component

[0065] This example verifies the efficacy of each component through animal immune function experiments and MTT tumor cell experiments.

[0066] Experimental group 1: same as Example 2 of the present invention;

[0067] Experimental group 2: compared with Example 2, selenium-rich garlic powder was removed, and 120 parts of maitake mushroom extract, 10 parts of ganoderma lucidum extract, 110 parts of cordyceps militaris extract, 20 parts of dandelion extract, 20 parts of broccoli extract, and 10 parts of turmeric were added;

[0068] Experimental group 3: compared with Example 2, the extract of Grifola frondosa was removed, and 205 parts of selenium-enriched garlic powder, 10 parts of Ganoderma lucidum extract, 25 parts of Cordyceps militaris extract, 20 parts of Dandelion extract, 20 parts of Broccoli extract, and 10 parts of Turmeric were added;

[0069] Experimental group 4: compared with Example 2, the Cordyceps militaris extract was removed, and 205 parts of selenium-enriched garlic powder, 25 parts of Grifola frondosa extract, 10 parts of Ganoderma lucidum extract, 20 parts of Taraxacum extract, 20 parts of Broccoli extract, and 10 parts of Turmeric were added.

[0070] Experimental group 5: components in the prior art: 10 parts of selenium-enriched broccoli, 15 parts of selenium-enriched shepherd's purse, 6 parts of selenium-enriched cabbage, 20 parts of konjac extract, 9 parts of dandelion extract, 9 parts of broccoli extract, and 10 parts of β-glucan.

[0071] Animal immune function experiment: 60 SPF rats weighing 200±20g were selected, and the animal immunodeficiency model was established with cyclophosphamide. The rats were randomly divided into 6 groups, with 10 rats in each group. The treatment conditions of each group were as follows:

[0072] Model group: rats were gavaged with normal saline;

[0073] Experimental group 1: rats were fed with the formula of implementation group 1;

[0074] Experimental group 2: The rats were fed with the formula of implementation group 2;

[0075] Experimental group 3: The rats were fed with the formula of implementation group 3;

[0076] Experimental group 4: The rats were fed with the formula of implementation group 4;

[0077] Experimental Group 5: Rats were fed with the formula of Group 5.

[0078] The thymus index, serum IgA content, and inflammatory factor TNF-α content of each group of mice were statistically analyzed; the results are shown in Table 2;

[0079] Table 2

[0080] Thymus index Serum IgA content Inflammatory factor TNF-α Model Group 1.17 40.34 121.61 Experimental Group 1 5.07 98.21 73.93 Experimental Group 2 2.49 60.42 108.52 Experimental Group 3 2.58 70.62 97.18 Experimental Group 4 2.61 74.83 102.52 Experimental Group 5 3.75 82.15 94.79

[0081] As can be seen from Table 2, the formula of the present invention has a significant effect of enhancing the function of the immune system and can eliminate inflammatory factors in the body. It should be noted that, from Experimental Groups 2 to 4, it can be seen that if the selenium-rich garlic powder, Cordyceps militaris extract or Grifola frondosa extract is removed arbitrarily, the effect of improving the immune system or removing the inflammatory factors is greatly reduced. This shows that the selenium-rich garlic powder, Cordyceps militaris extract and Grifola frondosa extract in the present invention have a synergistic effect.

[0082] Anti-tumor experiments

[0083] 7721 human liver cancer cell lines were selected, and experimental groups 1 to 5 were dissolved in serum-containing DMEM complete medium to prepare 2 mg / ml, and the survival rate of tumor cells in each group was counted. The results are shown in Table 3;

[0084] Table 3

[0085]

[0086]

[0087] The formula of the present invention has a significant effect of inhibiting tumor proliferation. It should be noted that, from experimental groups 2 to 4, it can be seen that the inhibitory effect on tumor cells is greatly reduced by removing any selenium-rich garlic powder, Cordyceps militaris extract or Grifola frondosa extract. This shows that the selenium-rich garlic powder, Cordyceps militaris extract and Grifola frondosa extract in the present invention have a synergistic effect.

[0088] The various embodiments are described in a progressive manner, and each embodiment focuses on the differences from other embodiments. The same or similar parts between the various embodiments can be referred to in detail.

[0089] The above description of the disclosed embodiments enables one skilled in the art to implement or use the present invention. Various modifications to these embodiments will be apparent to one skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but rather to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. An immune reconstruction compound for improving immune function, preventing and assisting in the treatment of tumors, characterized in that: The invention comprises the following components in parts by weight: 180-220 parts of selenium-enriched garlic powder, 15-25 parts of maitake mushroom extract, 7-13 parts of ganoderma lucidum extract, 9-12 parts of cordyceps militaris extract, 14-24 parts of dandelion extract, 17-25 parts of broccoli extract and 8-14 parts of turmeric.

2. The immune reconstitution compound for improving immune function, preventing and assisting in the treatment of tumors according to claim 1, characterized in that: The invention comprises the following components in optimal weight proportions: 200 parts of selenium-enriched garlic powder, 20 parts of maitake mushroom extract, 10 parts of ganoderma lucidum extract, 10 parts of cordyceps militaris extract, 20 parts of dandelion extract, 20 parts of broccoli extract and 10 parts of turmeric.

3. The method for preparing the immune reconstitution compound according to any one of claims 1 to 2, characterized in that the process include: S1. Weighing: weigh the raw materials according to a specific weight ratio; S2, ultrafinely grinding the turmeric to 600-800 mesh, sieving, and obtaining turmeric powder; S3, mixing and evenly stirring the Grifola frondosa extract, Ganoderma lucidum extract, Cordyceps militaris extract, Taraxacum officinale extract, and Broccoli extract to obtain a mixture 1; S4. Add turmeric powder to mixture 1, stir evenly, freeze-dry and pulverize to obtain a compound powder.

4. The method according to claim 3, characterized in that In S4, freeze drying is performed at -30 to -40°C for 1 to 2 hours.

5. The method according to claim 4, characterized in that In S4, the pulverization is ultrafine pulverization to 600-800 meshes.

6. Use of the compound prepared by the method according to claim 3 in preparing health products for auxiliary prevention or auxiliary treatment of anti-tumor.

7. Use of the compound prepared by the method according to claim 3 in preparing health products that assist in improving immunity and assist in eliminating anti-inflammatory and anti-inflammatory effects.

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