Organic trace element compound bag for improving eggshell quality and preparation method and application thereof

Abstract

The invention discloses an organic trace element compound bag for improving eggshell quality. The feed additive is prepared from the following raw materials in parts by mass: 100 to 200 parts of hydroxy methionine copper complex, 200 to 300 parts of methionine iron complex, 200 to 300 parts of glycine iron complex, 150 to 200 parts of hydroxy methionine zinc complex, 100 to 150 parts of hydroxy methionine manganese complex, 1 to 2 parts of vitamin E, 1 to 2 parts of nicotinamide, 1 to 2 parts of vitamin A acetate, 10 to 20 parts of vitamin D and 50 to 150 parts of carrier. The invention also discloses a preparation method and application thereof. The organic trace element composite bag is high in absorption and utilization rate, can remarkably improve the eggshell strength, has different degrees of promotion effects on the activity of carbonic anhydrase and SOD, and reduces the dark spot egg rate; the feed has the advantages of safety, high efficiency, low emission, environmental protection, good stability and the like, and has huge application potential in the field of laying fowl feeding.

Description

Technical Field

[0001] The invention belongs to the field of organic trace element composite packages, and relates to an organic trace element composite package for improving eggshell quality, a preparation method and application thereof. Background Art

[0002] Eggshell quality is one of the most important issues of concern to the poultry industry, affecting the economic benefits of laying hen production and egg hatching performance. High eggshell strength and few eggshell defects are important protective measures to prevent pathogens such as Salmonella from invading the eggshell. It is estimated that eggs with broken eggshells account for 6% to 10% of all eggs produced, causing huge economic losses. One of the main reasons is that the eggshell quality decreases as the hen ages, and the incidence of eggshell cracks may exceed 20% in the late egg production period.

[0003] Trace element nutrition and vitamins are closely related to the color and strength of eggshells. Common trace elements in the market generally have problems such as low absorption and utilization rate, high residual toxic and harmful substances, and unreasonable proportions, which cannot effectively meet the nutritional needs of laying poultry. Finding new safe, efficient, green and environmentally friendly alternatives has important economic and social benefits. Summary of the invention

[0004] The technical problem to be solved by the present invention is to overcome the deficiencies and defects mentioned in the above background technology and provide an organic trace element composite package for improving eggshell quality and a preparation method and application thereof.

[0005] In order to solve the above technical problems, the technical solution proposed by the present invention is: An organic trace element composite package for improving eggshell quality, the raw materials of which include the following components in mass fractions: 100-200 parts of hydroxymethionine copper complex, 200-300 parts of methionine iron complex, 200-300 parts of glycine iron complex, 150-200 parts of hydroxymethionine zinc complex, 100-150 parts of hydroxymethionine manganese complex, 1-2 parts of vitamin E, 1-2 parts of nicotinamide, 1-2 parts of vitamin A acetate, 10-20 parts of vitamin D and 50-150 parts of carrier. The above-mentioned complex is a chelate or complex compound, each component is compounded with a special carrier after being mixed, and is added to egg-laying poultry feed in an amount of 0.5‰-1.0‰. The product is safe, efficient, and low-emission, and can be used for large-scale industrial production. It provides a new type of organic trace element composite package for improving eggshell quality for egg-laying poultry feed, which can significantly reduce the egg breakage rate of egg-laying poultry in the late egg-laying period, improve the eggshell color, and reduce the dark spot egg rate.

[0006] The carrier of the organic trace element composite package is medical stone, and its particle size is ≤60 mesh.

[0007] Based on a general inventive concept, the present invention provides a method for preparing the above-mentioned organic trace element composite package, comprising the following steps: (1) mixing the hydroxymethionine copper complex, the methionine iron complex, the glycine iron complex, the hydroxymethionine zinc complex and the hydroxymethionine manganese complex uniformly to obtain a small package 1 for standby use; (2) Mix vitamin E, niacinamide, vitamin A acetate and vitamin D evenly to obtain packet 2 for later use; (3) The small package 1 obtained after step (1) and the small package 2 obtained after step (2) are uniformly mixed with a carrier to obtain an organic trace element composite package.

[0008] In the above preparation method, preferably, in step (1), step (2) and step (3), the mixing time is 120-180 seconds.

[0009] Based on a general inventive concept, the present invention also provides an application of the organic trace element compound package, wherein the organic trace element compound package is used as an additive to prepare feed for laying fowls, mixed evenly with feed, granulated and fed to laying fowls.

[0010] In the above application, preferably, the addition amount of the organic trace element compound package is 0.5‰-1.0‰ of the weight of the complete feed for laying fowls; more preferably 0.8‰, which has the best eggshell strength.

[0011] Compared with the prior art, the present invention has the following beneficial effects: 1. The organic trace element composite package of the present invention is mainly absorbed through the amino acid channel pathway, has a high absorption utilization rate, and has low emission. It can significantly improve the eggshell strength to achieve the purpose of reducing the egg breakage rate. It has different degrees of promotion on the activity of carbonic anhydrase and SOD, improves the eggshell color, and reduces the dark spot egg rate. Compared with additives with similar functions, it has the advantages of safety, high efficiency, low emission, environmental protection, good stability, etc., can be used for large-scale industrial production, and has great application potential in the field of laying poultry feeding.

[0012] 2. The organic trace element composite package of the present invention has low residual heavy metals and other toxic and harmful substances, is not easy to absorb moisture, has good stability, and can minimize the oxidative damage to other nutrients such as vitamins in feed, thereby ensuring the quality of feed.

[0013] 3. The preparation method of the present invention is simple, easy to operate, low in cost, scientific and environmentally friendly. DETAILED DESCRIPTION In order to facilitate the understanding of the present invention, the present invention will be described more comprehensively and carefully in combination with preferred embodiments below, but the protection scope of the present invention is not limited to the following specific embodiments.

[0014] Unless otherwise defined, all professional terms used below have the same meanings as those generally understood by those skilled in the art. The professional terms used herein are only for the purpose of describing specific embodiments and are not intended to limit the scope of protection of the present invention.

[0015] Unless otherwise specified, various raw materials, reagents, instruments and equipment used in the present invention can be purchased from the market or prepared by existing methods.

[0016] Embodiment 1: The invention discloses an organic trace element composite package for improving eggshell quality. The composite package is prepared from the following raw materials in parts by mass: 142.86 g / kg of hydroxymethionine copper complex, 250.00 g / kg of methionine iron complex, 235.30 g / kg of glycine iron complex, 166.67 g / kg of hydroxymethionine zinc complex, 133.33 g / kg of hydroxymethionine manganese complex, 200 IU / g of vitamin E, 200 IU / g of nicotinamide, 200 IU / g of vitamin A acetate, 100 IU / g of vitamin D and 71.84 g / kg of medical stone.

[0017] The preparation method of the organic trace element composite package specifically comprises the following steps: (1) Passing the hydroxymethionine copper complex, the methionine iron complex, the glycine iron complex, the hydroxymethionine zinc complex and the hydroxymethionine manganese complex through a 60-mesh sieve and mixing for 120 seconds to obtain a small package 1 for later use; (2) Passing vitamin E, niacinamide, vitamin A acetate and vitamin D through a 60-mesh sieve and mixing for 180 seconds to obtain packet 2 for later use; (3) Pass small package 1, small package 2 and modified medical stone through a 60-mesh sieve and mix for 120 seconds to obtain an organic trace element composite package.

[0018] Application Example 1: The organic trace element complex package prepared in Example 1 was added to the feed of 500-day-old Jingfen laying hens.

[0019] The experiment adopted a single-factor random group design, and selected 500-day-old Jingfen laying hens with similar production performance (egg production rate of about 90%, egg breaking rate of 2.0%), and randomly divided them into 4 groups, with 6 replicates in each group and 27 in each replicate. Among them, the control group did not add the organic trace element composite package prepared in Example 1 to the basic diet, and the basic diet was added with 6.4ppm of alkaline copper (in terms of copper element), 90ppm of ferrous sulfate (in terms of iron element), 70ppm of zinc sulfate (in terms of zinc element), 106ppm of manganese sulfate (in terms of manganese element), 0.3ppm of selenium (in terms of selenium element), and 0.8ppm of iodine (in terms of iodine element). The basic diet was designed with reference to the commercial formula; Group A, Group B and Group C respectively added 500g / T, 800g / T, and 1000g / T of the organic trace element composite package prepared in Example 1 to the basic diet. The experimental period was 70d.

[0020] Detection indicators: (1) Production performance: During the formal rearing period, the number of eggs laid, egg weight, number of soft-shelled eggs, and number of dead and culled chickens of each group (in replicates) were recorded daily, and the feed intake was counted weekly. The average daily feed intake, egg production rate, average egg weight, feed-egg ratio, daily egg production, and mortality and culling rates, as well as the rate of unqualified eggs, were calculated during the statistical period. The results are shown in Table 1.

[0021] (2) General egg quality: On the 35th and 70th days of the experiment, 6 eggs were randomly selected from each replicate for general egg quality analysis, and eggshell thickness, eggshell strength, egg shape index, yolk index, yolk ratio, yolk color, Haugh unit, and eggshell ratio were measured. The egg shape index (long diameter / short diameter of the egg) and yolk index (yolk height / yolk diameter) were measured using a digital caliper; the egg weight, yolk color, albumen height, and Haugh unit were measured using an Egg Analyzer produced by ORKA, Israel; and the eggshell thickness (blunt end, tip, and middle of the egg) was measured using an eggshell thickness tester; the results are shown in Table 2.

[0022] Table 1: Effects of different treatments on egg production performance of laying hens

[0023] As shown in Table 1, from week 1 to week 5, groups A, B and C delayed the downward trend of egg production in the late egg-laying period of laying hens compared with the control group, and significantly reduced the egg breakage rate by 44.90%, 63.75% and 48.47%, respectively.

[0024] From 6 to 10 weeks, groups A, B and C delayed the downward trend of egg production in the late egg-laying period and significantly reduced the egg breakage rate by 41.38%, % and 48.47% respectively compared with the control group.

[0025] From the perspective of the entire experimental period, groups A, B and C delayed the trend of egg production decline in the late laying period of laying hens and improved production performance compared with the control group, and significantly reduced the egg breakage rate. Compared with the control group, groups A, B and C reduced the egg breakage rate by 43.60%, 57.0% and 53.49% respectively.

[0026] Table 2: Effects of different treatments on egg quality of laying hens

[0027] The test results in the fifth week showed that compared with the control group, the eggshell strength of groups A, B, and C increased by 3.23%, 6.97%, and 4.73%, respectively, and the eggshell color was improved. The test results in the 10th week showed that compared with the control group, the eggshell strength of groups A, B, and C increased by 3.77%, 6.78%, and 4.27%, respectively, and the eggshell color was improved; As the amount of the organic trace element composite package prepared in Example 1 increases, when 500-1000 g / T is added, the eggshell quality is significantly improved, among which 800 g / T is the best. The test results show that adding the organic trace element composite package in an appropriate amount has the effect of improving the eggshell quality.

[0028] Application Example 2: The organic trace element complex package prepared in Example 1 was added to the feed of 450-day-old Hy-Line brown laying hens.

[0029] The experiment adopted a single-factor random group design, and 400-day-old Hailan brown laying hens with similar production performance (egg production rate of about 90%, egg breaking rate of 0.5%) were selected and randomly divided into 4 groups, with 6 replicates in each group and 30 in each replicate. Among them, the control group did not add the organic trace element composite package prepared in Example 1 to the basic diet, and the basic diet was added with 6.4ppm of alkaline copper (in terms of copper element), 90ppm of ferrous sulfate (in terms of iron element), 70ppm of zinc sulfate (in terms of zinc element), 106ppm of manganese sulfate (in terms of manganese element), 0.3ppm of selenium (in terms of selenium element), and 0.8ppm of iodine (in terms of iodine element). The basic diet was designed with reference to the commercial formula; the organic trace element composite package prepared in Example 1 was added to the basic diet in groups 1, 2 and 3, respectively, at 500g / T, 800g / T and 1000g / T, respectively. The experimental period was 4 weeks.

[0030] Detection indicators: (1) Determination of production performance indicators: The number of eggs laid, egg weight, number of broken eggs, number of deformed eggs, eggshell measurement (strength, thickness, color, measured once every 14 days) and number of dead and culled chickens were recorded repeatedly every day for each group, and the feed intake was counted every week; the average daily feed intake, egg production rate, average egg weight, feed-to-egg ratio, daily egg production and defective egg rate during the statistical period were calculated; the results are shown in Table 3.

[0031] (2) Eggshell quality determination: Two eggs were randomly collected from each replicate on days 14, 28, and 42 to test the eggshell quality: eggshell strength, eggshell thickness, and eggshell specific gravity. The results are shown in Table 4.

[0032] Table 3: Effects of different treatments on laying hen production performance

[0033] It can be seen from the data in Table 3 that in the early stage of the experiment 1-14 days, compared with the control group, treatments 1, 2, and 3 alleviated the downward trend of egg production in the late egg-laying period of laying hens, and each treatment had a significant downward trend in the egg breakage rate. Compared with the control group, treatments 1, 2, and 3 reduced the egg breakage rate by 44.90%, 52.04%, and 48.47%, respectively.

[0034] In the later stage of the experiment, 15-28 days, compared with the control group, treatments 1, 2, and 3 alleviated the downward trend of egg production in the late egg-laying period of laying hens, and each treatment had a significant trend of reducing the egg breakage rate. Compared with the control group, treatments 1, 2, and 3 reduced the egg breakage rate by 61.47%, 75.52%, and 73.71%, respectively.

[0035] Table 4: Effects of different treatments on eggshell quality

[0036] As can be seen from Table 4 above, the group adding 800g / T of the organic trace element composite package prepared in Example 1 has the best effect on improving eggshell quality. Compared with the control group, the eggshell color and eggshell strength of treatment groups 1, 2, and 3 are improved to a certain extent: when detected on the 14th day, the eggshell strength of treatment groups 1, 2, and 3 is increased by 5.8%, 11.59%, and 9.6% respectively compared with the control group. When detected on the 28th day, the eggshell strength of treatment groups 1, 2, and 3 is increased by 4.5%, 14.8%, and 11.52% respectively compared with the control group.

[0037] As the amount of the organic trace element composite package prepared in Example 1 increases, when 500-1000 g / T is added, the eggshell quality is significantly improved, among which 800 g / T is the best. The test results show that adding the organic trace element composite package in an appropriate amount has the effect of improving the eggshell quality.

[0038] Application Example 3 (Farm Application): 1. Test location and test time Experimental location: a large-scale laying hen farm in Dingzhou, Hebei; Test time: from December 13, 2017 to January 11, 2018, a total of 30 days.

[0039] 2. Experimental Design The No. 7 chicken house with poor eggshell color in the egg-laying chicken farm of this scale was selected as the experimental chicken house. One house was a treatment group, with a total of about 27,268 72-week-old Hy-Line brown laying hens. The test feed was an additional 1 kg / T of the organic trace element compound package prepared in Example 1 added to the basic diet. The eggshell color was mainly compared with that before and after the experiment in the No. 7 house. The management procedure was carried out in accordance with the daily production management of this farm. During the experiment, detailed records of medication, immunization and other abnormal conditions were kept. The formal period was 4 weeks.

[0040] 3. Test results Table 5: Effects of test materials on eggshell color

[0041] The test results are shown in Table 5. In the second week of using the organic trace element composite package of the present invention, the proportion of light-colored eggs decreased by an average of 27.1% compared with before the start of the test; in the fourth week of use, the proportion of light-colored eggs decreased by an average of 31.4% compared with before the start of the test; the on-site egg pickers reported that the eggshell color also improved. The age of the laying hens in the No. 7 henhouse is more than 500 days old. In actual production, no other nutritional health care is added. At this stage, the eggshell color of the laying hens will gradually become lighter as the age increases.

[0042] 4. Experimental conclusion Adding 1 kg / T of the organic trace element composite package prepared in Example 1 to the feed of 500-day-old laying hens in the late laying period can significantly improve the eggshell color.

[0043] The test results show that adding an appropriate amount of the organic trace element composite package prepared in Example 1 has the effect of improving the eggshell quality.

Claims

1. An organic trace element composite package for improving eggshell quality, characterized in that: The raw materials include the following components in parts by weight: 100-200 parts of hydroxymethionine copper complex, 200-300 parts of methionine iron complex, 200-300 parts of glycine iron complex, 150-200 parts of hydroxymethionine zinc complex, 100-150 parts of hydroxymethionine manganese complex, 1-2 parts of vitamin E, 1-2 parts of nicotinamide, 1-2 parts of vitamin A acetate, 10-20 parts of vitamin D and 50-150 parts of carrier.

2. The organic trace element composite package according to claim 1, characterized in that: The carrier is medical stone, and its particle size is ≤60 meshes.

3. A method for preparing an organic trace element composite package as claimed in claim 1 or 2, characterized in that: The steps include: (1) The hydroxymethionine copper complex, the methionine iron complex, the glycine iron complex, the hydroxymethionine zinc complex and the hydroxymethionine manganese complex are uniformly mixed to obtain a small package 1 for standby use; (2) Mix the vitamin E, niacinamide, vitamin A acetate and vitamin D evenly to obtain a small package 2 for later use; (3) The small package 1 obtained after the step (1) and the small package 2 obtained after the step (2) are uniformly mixed with the carrier to obtain the organic trace element composite package.

4. The preparation method according to claim 3, characterized in that: In the steps (1), (2) and (3), the mixing time is 120-180 seconds.

5. An application of the organic trace element composite package as claimed in claim 1 or 2 or the organic trace element composite package prepared by the preparation method as claimed in claim 3 or 4, characterized in that: The organic trace element composite package is used as an additive to prepare egg-laying poultry feed.

6. The use according to claim 5, characterized in that: The added amount of the organic trace element compound package is 0.5‰-1.0‰ of the weight of the complete feed for laying fowls.

7. The use according to claim 5 or 4, characterized in that: The added amount of the organic trace element compound package is 0.8‰ of the weight of the complete feed for laying fowls.

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