Method for improving purity of active collagen by using ultrasonic-assisted extraction technology

Abstract

The invention relates to the technical field of collagen detection, in particular to a method for improving the purity of active collagen by utilizing an ultrasonic-assisted extraction technology, which comprises the following steps of: judging whether a bubble release fluid space of collagen liquid is uniform or not according to the fluctuation degree of the liquid level height of the collagen liquid; according to the curve section overlap ratio of the absorption spectrum curves of different layers of samples in a plurality of wavelength sections, determining an abnormal section in the fluid, and judging whether the collagen liquid has an obvious fluid layering polymerization phenomenon or not by calculating a fluid layering polymerization characterization value; the ultrasonic extraction process of the collagen liquid is adjusted according to the overall curve overlap ratio of the absorption spectrum curves of the stirred samples in different layers, so that the interference of an active collagen liquid layering phenomenon caused by internal hydrodynamic state difference on the purity detection of the collagen liquid is eliminated; and the extraction process of the collagen liquid is effectively adjusted according to the accurate result of the purity detection of the collagen liquid.

Description

Technical Field

[0001] The present invention relates to the technical field of collagen detection, and in particular to a method for improving the purity of active collagen by utilizing ultrasound-assisted extraction technology. Background Art

[0002] As an important biomaterial, the research and application of active collagen are developing rapidly. The preparation process of active collagen mainly involves extraction through hot water extraction, acid-base hydrolysis and enzymatic hydrolysis. With the development of extraction technology and consumers' pursuit of high-quality life, active collagen has broad application prospects in medical beauty, beauty, health care and other fields.

[0003] Active collagen liquid has a wide range of applications in the fields of medical treatment, beauty and skin care. In order to ensure that the extracted active collagen liquid has a higher purity, the existing ultrasound-assisted extraction technology can significantly increase the yield of the target component. The triple helix structure of the active collagen molecule causes internal differences in the fluid dynamics of the active collagen liquid, which affects the accurate detection of the purity of the collagen liquid. Therefore, it is particularly important to detect and analyze the parameters that have data representation of the purity performance during the extraction process of the active collagen liquid.

[0004] For example, Chinese Patent Publication No.: CN117951484A, the invention discloses a method and system for detecting the quality of collagen tripeptide. The method first divides the product sampling data into product grouping data, performs bilateral filtering on each product grouping data, obtains each segmented filtered data, and then obtains product filtered data. In the process of calculating the adjusted weight of each reference data point through bilateral filtering, the initial weight is adjusted according to the data reliability value of the reference data point to obtain the adjusted weight of the reference data point, and the quality detection result of the collagen tripeptide is obtained according to the absorption spectrum filtering curve of all sampled samples.

[0005] The prior art still has the following problems:

[0006] The prior art does not consider that the stratification phenomenon caused by the difference in fluid dynamic state inside the extracted active collagen solution interferes with the accuracy of collagen solution purity detection, and the distorted collagen solution purity detection results affect the effectiveness of adjusting the collagen solution during the extraction process. Summary of the invention

[0007] To this end, the present invention provides a method for improving the purity of active collagen using ultrasound-assisted extraction technology, so as to overcome the problem that the prior art cannot eliminate the interference of the active collagen liquid stratification phenomenon caused by the internal fluid dynamic state difference on the collagen liquid purity detection, and cannot effectively adjust the extraction process of the collagen liquid.

[0008] To achieve the above object, the present invention provides a method for improving the purity of active collagen using ultrasound-assisted extraction technology, comprising:

[0009] Filling the collagen solution extracted by ultrasound into several packaging bottles, and judging whether the bubble release fluid space of the collagen solution is uniform based on the fluctuation degree of the liquid level of the collagen solution in the packaging bottles;

[0010] In response to the unevenness of the bubble-released fluid space of the collagen liquid, sampling information of the collagen liquid after a preset standing time is obtained, wherein the sampling information includes an absorption spectrum curve of an upper layer collagen liquid sample and an absorption spectrum curve of a lower layer collagen liquid sample;

[0011] Determine the curve segment overlap between the absorption spectrum curve of the upper collagen liquid sample and the absorption spectrum curve of the lower collagen liquid sample in a plurality of wavelength intervals, and determine the wavelength interval where the curve segment corresponding to the curve segment overlap that does not meet the preset standard is located as an abnormal interval in the fluid;

[0012] Calculating the fluid stratification and aggregation characterization value based on the number of abnormal intervals in the fluid and the overlap of curves corresponding to the abnormal intervals in each fluid, so as to determine whether the collagen liquid has obvious fluid stratification and aggregation phenomenon;

[0013] In response to the collagen liquid having obvious fluid stratification and polymerization phenomenon, the overall overlap of the absorption spectrum curve of the upper collagen liquid sample and the absorption spectrum curve of the lower collagen liquid sample after stirring the collagen liquid for a predetermined period of time is obtained, and whether the stratification and polymerization phenomenon of the collagen liquid is abnormal is determined based on the overall overlap of the curves.

[0014] Furthermore, the process of determining the degree of fluctuation of the liquid level of the collagen liquid in the packaging bottle is as follows:

[0015] The liquid level heights of the collagen liquid in the several packaging bottles are obtained, and the standard deviation of the liquid level heights is calculated according to the liquid level heights of the collagen liquid in the several packaging bottles.

[0016] Further, the process of determining whether the bubble-released fluid space of the collagen solution is uniform includes:

[0017] Comparing the liquid level standard deviation with a preset liquid level standard deviation threshold;

[0018] If the liquid level standard deviation is greater than the liquid level standard deviation threshold, it is determined that the bubble release fluid space of the collagen liquid is uneven.

[0019] Furthermore, the process of obtaining the sampling information includes:

[0020] Collecting an upper layer collagen liquid sample and a lower layer collagen liquid sample of the collagen liquid after the preset standing time;

[0021] Using a visible spectrometer to measure the absorption spectrum of the upper collagen liquid sample and the lower collagen liquid sample;

[0022] The absorption spectrum curve of the upper collagen liquid sample and the absorption spectrum curve of the lower collagen liquid sample are determined with the wavelength of light emitted by the visible spectrometer as the horizontal axis and the absorbance as the vertical axis.

[0023] Furthermore, the step of determining the curve segment overlap includes:

[0024] Dividing the absorption spectrum curve of the upper collagen liquid sample and the absorption spectrum curve of the lower collagen liquid sample into a plurality of wavelength intervals in the direction of the horizontal axis at the same preset wavelength interval;

[0025] Determine the absorption spectrum curve segment of the upper collagen liquid sample and the absorption spectrum curve segment of the lower collagen liquid sample in each wavelength interval segment;

[0026] The degree of overlap between the absorption spectrum curve segment of the upper collagen liquid sample and the absorption spectrum curve segment of the lower collagen liquid sample within the wavelength interval is calculated, and the degree of overlap is determined as the curve segment overlap degree.

[0027] Furthermore, the process of determining the abnormal interval section inside the fluid is as follows:

[0028] The curve segment coincidence degree is compared with a preset curve segment coincidence degree reference value. If the curve segment coincidence degree is less than or equal to the curve segment coincidence degree reference value, it is determined that the wavelength interval segment where the curve segment corresponding to the curve segment coincidence degree is located is an abnormal interval segment inside the fluid.

[0029] Furthermore, the fluid stratification aggregation characterization value is calculated according to the following formula:

[0030]

[0031] Wherein, M is the fluid stratification aggregation characterization value, k is the ratio of the number of abnormal intervals in the fluid to the number of wavelength intervals, k0 is the preset reference value of the number ratio, R av is the average value of the curve coincidence corresponding to the abnormal interval segment inside each fluid, R0 is the preset reference value of the curve coincidence average value, α is the preset quantity ratio weight coefficient, β is the preset curve coincidence weight coefficient, and e is a constant.

[0032] Furthermore, the process of determining whether the collagen solution has obvious fluid stratification and aggregation phenomenon includes:

[0033] Comparing the fluid stratified aggregation characterization value with a preset fluid stratified aggregation characterization value threshold;

[0034] If the fluid stratification and aggregation characterization value is greater than the fluid stratification and aggregation characterization value threshold, it is determined that the collagen solution has obvious fluid stratification and aggregation phenomenon.

[0035] Furthermore, the process of obtaining the overall overlap of the curves includes:

[0036] collecting an upper collagen liquid sample and a lower collagen liquid sample of the collagen liquid after stirring for a predetermined time;

[0037] Using a visible spectrometer to measure the absorption spectrum of the upper collagen liquid sample and the lower collagen liquid sample, and determine the absorption spectrum curve of the upper collagen liquid sample and the absorption spectrum curve of the lower collagen liquid sample;

[0038] The degree of overlap between the absorption spectrum curve of the upper collagen liquid sample and the absorption spectrum curve of the lower collagen liquid sample is calculated, and the degree of overlap is determined as the overall overlap of the curves.

[0039] Furthermore, the process of adjusting the ultrasonic power and the duration of the ultrasonic extraction of the collagen solution includes:

[0040] Comparing the overall curve coincidence with a preset reference value of the overall curve coincidence;

[0041] If the overall coincidence of the curves is greater than or equal to the overall coincidence reference value of the curves, it is determined that no adjustment is made to the ultrasonic extraction of the collagen solution;

[0042] If the overall overlap of the curves is less than the overall overlap reference value of the curves, adjusting the ultrasonic power and the duration of the ultrasonic extraction of the collagen solution;

[0043] Therein, the ultrasonic power is negatively correlated with the overall coincidence of the curve, and the ultrasonic duration is negatively correlated with the overall coincidence of the curve.

[0044] Compared with the prior art, the beneficial effect of the present invention lies in that the present invention determines whether the bubble release fluid space of the collagen liquid is uniform according to the degree of fluctuation of the liquid level of the collagen liquid in several packaging bottles, determines the curve segment overlap of the absorption spectrum curve of the upper collagen liquid sample and the absorption spectrum curve of the lower collagen liquid sample in several wavelength intervals according to the sampling information of the collagen liquid after a preset static time, so as to determine the abnormal interval segment inside the fluid, and calculates the fluid stratification aggregation characterization value according to the number of abnormal interval segments inside the fluid and the corresponding curve overlap, so as to determine whether the collagen liquid has obvious flow Finally, the ultrasonic extraction process of the collagen liquid was adjusted according to the overall coincidence of the absorption spectrum curve of the upper collagen liquid sample and the absorption spectrum curve of the lower collagen liquid sample after the collagen liquid was stirred for a predetermined time. Furthermore, the fluid dynamics state of the collagen liquid was determined according to the release of bubbles inside the collagen liquid, and the interference of the stratification phenomenon of the active collagen liquid caused by the difference in the internal fluid dynamics state on the purity detection of the collagen liquid was eliminated. According to the accurate results of the purity detection of the collagen liquid, the extraction process of the collagen liquid was effectively adjusted.

[0045] In particular, the present invention determines whether the bubble release fluid space of the collagen liquid is uniform based on the degree of fluctuation of the liquid level of the collagen liquid that has been filled in several packaging bottles. Those skilled in the art can understand that the triple helix structure of the active collagen molecule causes the collagen liquid to be viscous, and there are internal differences in the fluid dynamics state generated by the impact of the viscous liquid fluid during the filling process, resulting in uneven distribution of bubbles generated in the collagen liquid during the filling process. The different rates at which the unevenly distributed bubbles precipitate out of the collagen liquid result in different degrees of drop in the liquid level of the collagen liquid after the bubbles are precipitated. The greater the degree of fluctuation of the liquid level of the collagen liquid that has been filled, the greater the internal difference that characterizes the fluid dynamics state of the collagen liquid. Furthermore, the fluid dynamics state of the collagen liquid is determined based on the release of bubbles inside the collagen liquid, thereby improving the detection accuracy.

[0046] In particular, the present invention determines the overlap of the absorption spectrum curve of the upper collagen liquid sample with the absorption spectrum curve of the lower collagen liquid sample in several wavelength ranges after the collagen liquid is allowed to stand for a preset standing time. Those skilled in the art will understand that if there are impurities or uneven aggregation distribution of protein molecules inside the collagen liquid, there will be a difference in the impurity concentration or protein molecule concentration between the upper collagen liquid sample and the lower collagen liquid sample during the standing process. Impurities or collagen molecules in different aggregation states affect the absorbance of the collagen liquid sample at a specific wavelength. The present invention can clearly determine the purity difference between the upper collagen liquid sample and the lower collagen liquid sample by calculating the overlap of the absorption spectrum curve of the upper collagen liquid sample with the absorption spectrum curve of the lower collagen liquid sample in several wavelength ranges, thereby achieving accurate detection of collagen liquids with different fluid dynamic states.

[0047] In particular, the present invention calculates the fluid stratification aggregation characterization value by the number of abnormal interval segments inside the fluid and the curve overlap corresponding to each abnormal interval segment inside the fluid. In the actual curve segment overlap comparison process of the absorption spectrum curve of the collagen liquid sample, the more wavelength interval segments whose curve segment overlap does not meet the preset standard, the greater the degree of difference between the upper collagen liquid sample and the lower collagen liquid sample. Similarly, the smaller the average value of the curve overlap corresponding to several abnormal interval segments inside the fluid, the greater the degree of difference between the upper collagen liquid sample and the lower collagen liquid sample. Thus, the intuitive quantification of the stratification difference degree of the collagen liquid samples is achieved, the detection accuracy of the collagen liquid with different fluid dynamic states is improved, and the interference of the stratification phenomenon of the active collagen liquid caused by the internal fluid dynamic state difference on the collagen liquid purity detection is eliminated.

[0048] In particular, the present invention adjusts the ultrasonic extraction process of the collagen liquid according to the overall coincidence of the absorption spectrum curve of the upper collagen liquid sample and the absorption spectrum curve of the lower collagen liquid sample after the collagen liquid is stirred for a predetermined period of time. Those skilled in the art can understand that if the stratification difference of the collagen liquid samples is due to the different aggregation concentrations of protein molecules caused by the difference in the fluid dynamics state of the collagen liquid, the stratification difference of the collagen liquid samples can be significantly reduced by stirring the collagen liquid, that is, the overall coincidence of the absorption spectrum curves of the upper collagen liquid sample and the lower collagen liquid sample is high, then the stratification and aggregation phenomenon of the collagen liquid is not caused by impurities, and there is no need to perform ultrasonic extraction of the collagen liquid. Adjustment is made. If the stratification difference of the collagen liquid sample is caused by impurities in the collagen liquid, stirring the collagen liquid cannot significantly reduce the stratification difference of the collagen liquid sample, that is, the overall overlap of the absorption spectrum curves of the upper collagen liquid sample and the lower collagen liquid sample is low. It is necessary to increase the ultrasonic power of the collagen liquid during ultrasonic extraction and extend the ultrasonic duration of the collagen liquid during ultrasonic extraction to improve the purity of the collagen liquid during the extraction process, and then eliminate the interference of the stratification phenomenon of the active collagen liquid caused by the difference in the internal fluid dynamic state on the purity detection of the collagen liquid, and effectively adjust the extraction process of the collagen liquid according to the accurate results of the collagen liquid purity detection. BRIEF DESCRIPTION OF THE DRAWINGS

[0049] Figure 1 This is a step diagram of a method for improving the purity of active collagen using ultrasound-assisted extraction technology according to an embodiment of the present invention;

[0050] Figure 2 A diagram showing the steps of obtaining sampling information according to an embodiment of the present invention;

[0051] Figure 3 A diagram showing the steps of determining the overlap of curve segments according to an embodiment of the present invention;

[0052] Figure 4 This is a logic flow chart for determining whether the collagen solution has obvious fluid stratification and aggregation phenomenon according to an embodiment of the present invention. DETAILED DESCRIPTION

[0053] In order to make the objects and advantages of the present invention more clearly understood, the present invention is further described below in conjunction with embodiments; it should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0054] The preferred embodiments of the present invention are described below with reference to the accompanying drawings. It should be understood by those skilled in the art that these embodiments are only used to explain the technical principles of the present invention and are not intended to limit the protection scope of the present invention.

[0055] It should be noted that, in the description of the present invention, terms such as "upper", "lower", "inside" and "outside" indicating directions or positional relationships are based on the directions or positional relationships shown in the drawings. This is only for the convenience of description and does not indicate or imply that the device or element must have a specific orientation, be constructed and operated in a specific orientation. Therefore, it cannot be understood as a limitation on the present invention.

[0056] See also Figure 1 As shown, it is a step diagram of a method for improving the purity of active collagen by using ultrasound-assisted extraction technology according to an embodiment of the present invention. A method for improving the purity of active collagen by using ultrasound-assisted extraction technology according to the present invention comprises:

[0057] Step S100, filling the collagen solution extracted by ultrasound into a plurality of packaging bottles, and determining whether the bubble release fluid space of the collagen solution is uniform based on the degree of fluctuation of the liquid level of the collagen solution in the packaging bottles;

[0058] Step S200, in response to the uneven space of the bubble-released fluid of the collagen liquid, obtaining sampling information of the collagen liquid after a preset standing time, the sampling information including an absorption spectrum curve of the upper collagen liquid sample and an absorption spectrum curve of the lower collagen liquid sample;

[0059] Step S300, determining the curve segment overlap between the absorption spectrum curve of the upper collagen liquid sample and the absorption spectrum curve of the lower collagen liquid sample in a plurality of wavelength intervals, and determining the wavelength interval where the curve segment corresponding to the curve segment overlap that does not meet the preset standard is located as an abnormal interval in the fluid;

[0060] Step S400, calculating the fluid stratification and aggregation characterization value based on the number of abnormal intervals in the fluid and the overlap of curves corresponding to the abnormal intervals in the fluid, so as to determine whether the collagen liquid has obvious fluid stratification and aggregation phenomenon;

[0061] Step S500, in response to the collagen liquid having obvious fluid stratification and polymerization phenomenon, obtain the overall overlap of the absorption spectrum curve of the upper collagen liquid sample and the absorption spectrum curve of the lower collagen liquid sample after the collagen liquid is stirred for a predetermined period of time, and determine whether the stratification and polymerization phenomenon of the collagen liquid is abnormal based on the overall overlap of the curves.

[0062] Specifically, those skilled in the art can understand that the ultrasonic-assisted extraction technology can improve the extraction efficiency and purity of collagen in the extraction of active collagen, by mixing the treated raw materials with an appropriate amount of solvent, ultrasonically treating the mixed solvent with a preset ultrasonic power and a preset ultrasonic duration to promote the release of collagen, and centrifuging the solvent after ultrasonic treatment and enzymatic hydrolysis to obtain a collagen liquid. The ultrasonic power and ultrasonic duration are parameters commonly used by those skilled in the art in the art in ultrasonic-assisted extraction technology, and will not be repeated here.

[0063] Specifically, in an embodiment of the present invention, the liquid level of the collagen liquid filled in several packaging bottles can be obtained through a visual system. The visual system with a high-definition camera is widely used in liquid level detection in the food and pharmaceutical industries. This is a prior art and will not be elaborated here.

[0064] In an embodiment of the present invention, the preset standing time of the collagen solution is preset by technicians in this field based on the standing time required for the collagen solution to achieve internal material stratification. Preferably, the preset standing time can range from [1,6], and the interval unit is h.

[0065] Specifically, in an embodiment of the present invention, the Euclidean distance between corresponding points on the two curves can be calculated by an existing algorithm, and then all distances are summed. The smaller the obtained value, the higher the overlap of the two curves. The overlap of the absorption spectrum curve of the upper collagen liquid sample and the absorption spectrum curve of the lower collagen liquid sample in several wavelength intervals and the overall overlap of the absorption spectrum curve of the upper collagen liquid sample and the absorption spectrum curve of the lower collagen liquid sample can be calculated accordingly. The calculation of curve overlap is a technical means commonly used in curve analysis and will not be repeated here.

[0066] Specifically, the process of determining the degree of fluctuation of the liquid level of the collagen liquid in the packaging bottle is as follows:

[0067] Obtain the liquid level of the collagen solution in several packaging bottles, and calculate the standard deviation h of the liquid level according to the liquid level of the collagen solution in several packaging bottles c .

[0068] Specifically, the process of determining whether the bubble-released fluid space of the collagen solution is uniform includes:

[0069] The standard deviation of the liquid level h c The preset liquid level height standard deviation threshold h c0 Make a comparison;

[0070] If the standard deviation of the liquid level h c Less than or equal to the liquid level height standard deviation threshold hc0 , it is determined that the bubble-released fluid space of the collagen solution is uniform;

[0071] If the standard deviation of the liquid level h c Greater than the liquid level standard deviation threshold h c0 , it is determined that the bubble-released fluid space of the collagen solution is uneven.

[0072] Preferably, in the embodiment of the present invention, the liquid level standard deviation threshold h c0 The value of is determined according to the height H of the packaging bottle, h c0 =δ×H, δ is the value factor of the standard deviation threshold of the liquid level height, and the value range of δ is [0.08, 0.1].

[0073] Specifically, the present invention determines whether the bubble release fluid space of the collagen liquid is uniform according to the degree of fluctuation of the liquid level of the collagen liquid that has been filled in several packaging bottles. Those skilled in the art can understand that the triple helix structure of the active collagen molecule causes the collagen liquid to be viscous, and there are internal differences in the fluid dynamics state generated by the impact of the viscous liquid fluid during the filling process, resulting in uneven distribution of bubbles generated in the collagen liquid during the filling process. The different rates at which the unevenly distributed bubbles precipitate out of the collagen liquid result in different degrees of drop in the liquid level of the collagen liquid after the bubbles are precipitated. The greater the fluctuation of the liquid level of the collagen liquid that has been filled, the greater the internal difference that characterizes the fluid dynamics state of the collagen liquid. Furthermore, the fluid dynamics state of the collagen liquid is determined according to the release of bubbles inside the collagen liquid, thereby improving the detection accuracy.

[0074] Specifically, see Figure 2 As shown, it is a step diagram of obtaining sampling information according to an embodiment of the present invention. The process of obtaining the sampling information includes:

[0075] Step S201, collecting an upper layer collagen liquid sample and a lower layer collagen liquid sample of the collagen liquid after a preset standing time;

[0076] Step S202, using a visible spectrometer to measure the absorption spectra of the upper collagen liquid sample and the lower collagen liquid sample;

[0077] Step S203, determining the absorption spectrum curve of the upper collagen liquid sample and the absorption spectrum curve of the lower collagen liquid sample with the wavelength of the light emitted by the visible spectrometer as the horizontal axis and the absorbance as the vertical axis.

[0078] Specifically, in the embodiment of the present invention, the absorption of light of a specific wavelength by the collagen solution can be measured by an ultraviolet-visible spectrometer. This is a prior art and will not be described in detail here.

[0079] Specifically, see Figure 3 As shown, it is a step diagram of determining the coincidence degree of curve segments according to an embodiment of the present invention, and the step of determining the coincidence degree of curve segments includes:

[0080] Step S301, dividing the absorption spectrum curve of the upper collagen solution sample and the absorption spectrum curve of the lower collagen solution sample into a plurality of wavelength intervals in the direction of the horizontal axis at the same preset wavelength interval;

[0081] Step S302, determining the absorption spectrum curve segments of the upper collagen solution sample and the absorption spectrum curve segments of the lower collagen solution sample within each wavelength interval;

[0082] Step S303, calculating the overlap between the absorption spectrum curve segment of the upper collagen liquid sample and the absorption spectrum curve segment of the lower collagen liquid sample within the wavelength interval, and determining the overlap as the curve segment overlap.

[0083] Preferably, in the embodiment of the present invention, the value range of the preset wavelength interval is [20, 30], and the interval unit is nm.

[0084] Specifically, in an embodiment of the present invention, the Euclidean distance between corresponding points on two curves can be calculated by an existing algorithm, and then all distances are summed. The smaller the value obtained, the higher the degree of overlap of the curve segments. The calculation of the degree of overlap of curve segments is a commonly used technical means in curve analysis and will not be repeated here.

[0085] Specifically, the present invention allows the collagen liquid to stand for a preset standing time, and then determines the degree of overlap between the absorption spectrum curve of the upper collagen liquid sample and the absorption spectrum curve of the lower collagen liquid sample in several wavelength ranges. Those skilled in the art will understand that if there are impurities or uneven aggregation distribution of protein molecules inside the collagen liquid, there will be a difference in the impurity concentration or protein molecule concentration between the upper collagen liquid sample and the lower collagen liquid sample during the standing process. Impurities or collagen molecules in different aggregation states affect the absorbance of the collagen liquid sample at a specific wavelength. The present invention can clearly determine the purity difference between the upper collagen liquid sample and the lower collagen liquid sample by calculating the degree of overlap between the absorption spectrum curve of the upper collagen liquid sample and the absorption spectrum curve of the lower collagen liquid sample in several wavelength ranges, thereby achieving accurate detection of collagen liquids with different fluid dynamic states.

[0086] Specifically, the process of determining the abnormal interval section inside the fluid is as follows:

[0087] The curve segment overlap R is compared with the preset curve segment overlap reference value Ra If the curve segment overlap R is less than or equal to the curve segment overlap reference value R a , then it is determined that the wavelength interval where the curve segment corresponding to the curve segment coincidence degree is located is the abnormal interval inside the fluid.

[0088] Preferably, in the embodiment of the present invention, the preset curve segment overlap reference value R a The value range is [88%, 93%].

[0089] Specifically, the fluid stratification aggregation characterization value is calculated according to the following formula:

[0090]

[0091] Wherein, M is the fluid stratification aggregation characterization value, k is the ratio of the number of abnormal intervals in the fluid to the number of wavelength intervals, k0 is the preset reference value of the number ratio, R av is the average value of the curve coincidence corresponding to the abnormal interval segment inside each fluid, R0 is the preset reference value of the curve coincidence average value, α is the preset quantity ratio weight coefficient, β is the preset curve coincidence weight coefficient, α+β=1, and e is a constant.

[0092] Preferably, in the embodiment of the present invention, k=n1 / n2, wherein n1 is the number of abnormal intervals in the fluid, n2 is the number of wavelength intervals, the preset number ratio reference value k0 has a value range of [0.15, 0.2], and the preset curve coincidence average value reference value R0 has a value range of [0.9R a , 0.95R a ].

[0093] The quantity ratio weight coefficient α and the curve coincidence weight coefficient β are selected by technical personnel in this field based on the ratio of the number of abnormal interval segments inside the fluid to the number of wavelength interval segments in historical data and the influence of the curve coincidence on the calculation results. Preferably, the quantity ratio weight coefficient α can be set to 0.4, and the curve coincidence weight coefficient β can be set to 0.6.

[0094] Specifically, the present invention calculates the fluid stratification aggregation characterization value by the number of abnormal interval segments inside the fluid and the curve overlap corresponding to each abnormal interval segment inside the fluid. In the actual curve segment overlap comparison process of the absorption spectrum curve of the collagen liquid sample, the more wavelength interval segments whose curve segment overlap does not meet the preset standard, the greater the degree of difference between the upper collagen liquid sample and the lower collagen liquid sample. Similarly, the smaller the average value of the curve overlap corresponding to several abnormal interval segments inside the fluid, the greater the degree of difference between the upper collagen liquid sample and the lower collagen liquid sample. Thus, the intuitive quantification of the stratification difference degree of the collagen liquid samples is achieved, the detection accuracy of the collagen liquid with different fluid dynamic states is improved, and the interference of the stratification phenomenon of the active collagen liquid caused by the internal fluid dynamic state difference on the collagen liquid purity detection is eliminated.

[0095] Specifically, see Figure 4 As shown, it is a logic flow chart of determining whether the collagen solution has obvious fluid stratification and polymerization phenomenon according to an embodiment of the present invention. The process of determining whether the collagen solution has obvious fluid stratification and polymerization phenomenon includes:

[0096] Comparing the fluid stratified aggregation characterization value M with a preset fluid stratified aggregation characterization value threshold M0;

[0097] If the fluid stratification and aggregation characterization value M is less than or equal to the fluid stratification and aggregation characterization value threshold M0, it is determined that the collagen solution has no obvious fluid stratification and aggregation phenomenon;

[0098] If the fluid stratification and aggregation characterization value M is greater than the fluid stratification and aggregation characterization value threshold M0, it is determined that the collagen solution has obvious fluid stratification and aggregation phenomenon.

[0099] Preferably, in the embodiment of the present invention, the value range of the fluid stratified aggregation characterization value threshold M0 is [2.9, 2.95].

[0100] Specifically, the process of obtaining the overall coincidence of the curves includes:

[0101] collecting an upper collagen liquid sample and a lower collagen liquid sample of the collagen liquid after stirring for a predetermined time;

[0102] Using a visible spectrometer to measure the absorption spectrum of the upper collagen liquid sample and the lower collagen liquid sample, and determine the absorption spectrum curve of the upper collagen liquid sample and the absorption spectrum curve of the lower collagen liquid sample;

[0103] The degree of overlap between the absorption spectrum curve of the upper collagen liquid sample and the absorption spectrum curve of the lower collagen liquid sample is calculated, and the degree of overlap is determined as the overall overlap of the curves.

[0104] Specifically, the Euclidean distance between corresponding points on two curves can be calculated through existing algorithms, and then all distances can be summed up. The smaller the value obtained, the higher the overall coincidence of the curves. The calculation of the overall coincidence of curves is a commonly used technical means in curve analysis and will not be repeated here.

[0105] Specifically, the process of adjusting the ultrasonic power and the duration of the ultrasonic extraction of the collagen solution includes:

[0106] The overall curve coincidence R' is compared with the preset overall curve coincidence reference value R b Make a comparison;

[0107] If the overall overlap of the curve R' is greater than or equal to the overall overlap reference value R b , it is determined that the ultrasonic extraction of the collagen solution is not adjusted;

[0108] If the overall overlap of the curve R' is less than the overall overlap reference value R b , the ultrasonic power and the duration of the ultrasonic extraction of the collagen solution are adjusted;

[0109] Therein, the ultrasonic power is negatively correlated with the overall coincidence of the curve, and the ultrasonic duration is negatively correlated with the overall coincidence of the curve.

[0110] Preferably, in the embodiment of the present invention, the overall curve overlap reference value R b The value range is [90%, 93%]. The ultrasonic power and the ultrasonic duration are parameters commonly used by technicians in the field of ultrasonic-assisted extraction technology. Technicians in the field can initially set the ultrasonic power according to the type of collagen solution to be extracted, and adjust it based on the initially set ultrasonic power. For example, in the collagen solution extraction process of sheepskin, the value range of the initially set ultrasonic power can be [210, 230], and the unit of the interval is W. Similarly, technicians in the field can also initially set the ultrasonic duration according to the type of collagen solution to be extracted, and adjust it based on the initially set ultrasonic duration. For example, in the collagen solution extraction process of sheepskin, the value range of the initially set ultrasonic duration is [20, 30], and the unit of the interval is min.

[0111] Specifically, the present invention adjusts the ultrasonic extraction process of the collagen liquid according to the overall overlap of the absorption spectrum curve of the upper collagen liquid sample and the absorption spectrum curve of the lower collagen liquid sample after the collagen liquid is stirred for a predetermined period of time. Those skilled in the art can understand that if the stratification difference of the collagen liquid sample is due to the different aggregation concentrations of protein molecules caused by the difference in the fluid dynamics state of the collagen liquid, the stratification difference of the collagen liquid sample can be significantly reduced by stirring the collagen liquid, that is, the overall overlap of the absorption spectrum curves of the upper collagen liquid sample and the lower collagen liquid sample is high, then the stratification and aggregation phenomenon of the collagen liquid is not caused by impurities, and ultrasonic extraction of the collagen liquid is not necessary. Adjustment is made. If the stratification difference of the collagen liquid sample is caused by impurities in the collagen liquid, stirring the collagen liquid cannot significantly reduce the stratification difference of the collagen liquid sample, that is, the overall overlap of the absorption spectrum curves of the upper collagen liquid sample and the lower collagen liquid sample is low. It is necessary to increase the ultrasonic power of the collagen liquid during ultrasonic extraction and extend the ultrasonic duration of the collagen liquid during ultrasonic extraction to improve the purity of the collagen liquid during the extraction process, and then eliminate the interference of the stratification phenomenon of the active collagen liquid caused by the difference in the internal fluid dynamic state on the purity detection of the collagen liquid, and effectively adjust the extraction process of the collagen liquid according to the accurate results of the collagen liquid purity detection.

[0112] So far, the technical solutions of the present invention have been described in conjunction with the preferred embodiments shown in the accompanying drawings. However, it is easy for those skilled in the art to understand that the protection scope of the present invention is obviously not limited to these specific embodiments. Without departing from the principle of the present invention, those skilled in the art can make equivalent changes or substitutions to the relevant technical features, and the technical solutions after these changes or substitutions will fall within the protection scope of the present invention.

[0113] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A method for improving the purity of active collagen using ultrasound-assisted extraction technology, characterized in that: include: Filling the collagen solution extracted by ultrasound into several packaging bottles, and judging whether the bubble release fluid space of the collagen solution is uniform based on the fluctuation degree of the liquid level of the collagen solution in the packaging bottles; In response to the unevenness of the bubble-released fluid space of the collagen liquid, sampling information of the collagen liquid after a preset standing time is obtained, wherein the sampling information includes an absorption spectrum curve of an upper layer collagen liquid sample and an absorption spectrum curve of a lower layer collagen liquid sample; Determine the curve segment overlap between the absorption spectrum curve of the upper collagen liquid sample and the absorption spectrum curve of the lower collagen liquid sample in a plurality of wavelength intervals, and determine the wavelength interval where the curve segment corresponding to the curve segment overlap that does not meet the preset standard is located as an abnormal interval in the fluid; Calculating the fluid stratification and aggregation characterization value based on the number of abnormal intervals in the fluid and the overlap of curves corresponding to the abnormal intervals in each fluid, so as to determine whether the collagen liquid has obvious fluid stratification and aggregation phenomenon; In response to the obvious fluid stratification and aggregation phenomenon of the collagen liquid, the overall overlap of the absorption spectrum curve of the upper collagen liquid sample and the absorption spectrum curve of the lower collagen liquid sample after stirring the collagen liquid for a predetermined period of time is obtained, and the ultrasonic power and ultrasonic duration of the collagen liquid during the ultrasonic extraction process are adjusted according to the overall overlap of the curves.

2. The method for improving the purity of active collagen using ultrasound-assisted extraction technology according to claim 1, characterized in that: The process of determining the degree of fluctuation of the liquid level of the collagen solution in the packaging bottle is as follows: The liquid level heights of the collagen liquid in the several packaging bottles are obtained, and the standard deviation of the liquid level heights is calculated according to the liquid level heights of the collagen liquid in the several packaging bottles.

3. The method for improving the purity of active collagen by using ultrasound-assisted extraction technology according to claim 2, characterized in that: The process of determining whether the bubble-released fluid space of the collagen solution is uniform includes: Comparing the liquid level standard deviation with a preset liquid level standard deviation threshold; If the liquid level standard deviation is greater than the liquid level standard deviation threshold, it is determined that the bubble release fluid space of the collagen liquid is uneven.

4. The method for improving the purity of active collagen using ultrasound-assisted extraction technology according to claim 3, characterized in that: The process of obtaining the sampling information includes: Collecting an upper layer collagen liquid sample and a lower layer collagen liquid sample of the collagen liquid after the preset standing time; Using a visible spectrometer to measure the absorption spectrum of the upper collagen liquid sample and the lower collagen liquid sample; The absorption spectrum curve of the upper collagen liquid sample and the absorption spectrum curve of the lower collagen liquid sample are determined with the wavelength of light emitted by the visible spectrometer as the horizontal axis and the absorbance as the vertical axis.

5. The method for improving the purity of active collagen using ultrasound-assisted extraction technology according to claim 4, characterized in that: The step of determining the curve segment overlap comprises: Dividing the absorption spectrum curve of the upper collagen liquid sample and the absorption spectrum curve of the lower collagen liquid sample into a plurality of wavelength intervals in the direction of the horizontal axis at the same preset wavelength interval; Determine the absorption spectrum curve segment of the upper collagen liquid sample and the absorption spectrum curve segment of the lower collagen liquid sample in each wavelength interval segment; The degree of overlap between the absorption spectrum curve segment of the upper collagen liquid sample and the absorption spectrum curve segment of the lower collagen liquid sample within the wavelength interval is calculated, and the degree of overlap is determined as the curve segment overlap degree.

6. The method for improving the purity of active collagen using ultrasound-assisted extraction technology according to claim 5, characterized in that: The process of determining the abnormal interval section inside the fluid is as follows: The curve segment coincidence degree is compared with a preset curve segment coincidence degree reference value. If the curve segment coincidence degree is less than or equal to the curve segment coincidence degree reference value, it is determined that the wavelength interval segment where the curve segment corresponding to the curve segment coincidence degree is located is an abnormal interval segment inside the fluid.

7. The method for improving the purity of active collagen using ultrasound-assisted extraction technology according to claim 6, characterized in that: The fluid stratification aggregation characterization value is calculated according to the following formula: Wherein, M is the fluid stratification aggregation characterization value, k is the ratio of the number of abnormal intervals in the fluid to the number of wavelength intervals, k0 is the preset reference value of the number ratio, R av is the average value of the curve coincidence corresponding to the abnormal interval segment inside each fluid, R0 is the preset reference value of the curve coincidence average value, α is the preset quantity ratio weight coefficient, β is the preset curve coincidence weight coefficient, and e is a constant.

8. The method for improving the purity of active collagen using ultrasound-assisted extraction technology according to claim 7, characterized in that: The process of determining whether the collagen solution has obvious fluid stratification and aggregation phenomenon includes: Comparing the fluid stratified aggregation characterization value with a preset fluid stratified aggregation characterization value threshold; If the fluid stratification and aggregation characterization value is greater than the fluid stratification and aggregation characterization value threshold, it is determined that the collagen solution has obvious fluid stratification and aggregation phenomenon.

9. The method for improving the purity of active collagen using ultrasound-assisted extraction technology according to claim 8, characterized in that: The process of obtaining the overall coincidence of the curves includes: collecting an upper collagen liquid sample and a lower collagen liquid sample of the collagen liquid after stirring for a predetermined time; Using a visible spectrometer to measure the absorption spectrum of the upper collagen liquid sample and the lower collagen liquid sample, and determine the absorption spectrum curve of the upper collagen liquid sample and the absorption spectrum curve of the lower collagen liquid sample; The degree of overlap between the absorption spectrum curve of the upper collagen liquid sample and the absorption spectrum curve of the lower collagen liquid sample is calculated, and the degree of overlap is determined as the overall overlap of the curves.

10. The method for improving the purity of active collagen using ultrasound-assisted extraction technology according to claim 9, characterized in that: The process of adjusting the ultrasonic power and the duration of the ultrasonic extraction of the collagen solution includes: Comparing the overall curve coincidence with a preset reference value of the overall curve coincidence; If the overall coincidence of the curves is greater than or equal to the overall coincidence reference value of the curves, it is determined that no adjustment is made to the ultrasonic extraction of the collagen solution; If the overall overlap of the curves is less than the overall overlap reference value of the curves, adjusting the ultrasonic power and the duration of the ultrasonic extraction of the collagen solution; Therein, the ultrasonic power is negatively correlated with the overall coincidence of the curve, and the ultrasonic duration is negatively correlated with the overall coincidence of the curve.

Images