Cell solution mixing structure

Abstract

The invention discloses a cell solution mixing structure which comprises a feeding cavity and a test tube, the feeding cavity comprises a sample injection needle through hole, a sample injection needle and a mounting hole, the sample injection needle through hole is formed in the feeding cavity in the vertical direction of the feeding cavity, the sample injection needle penetrates and is inserted into the sample injection needle through hole, the top of the sample injection needle is limited and fixed in the sample injection needle through hole through a sealing joint, and the test tube is arranged in the mounting hole. The mounting hole is formed in the feeding cavity in the transverse direction of the feeding cavity and is communicated with the sample injection needle through hole; the cell solution uniform mixing structure further comprises a vibration structure, the vibration structure is arranged in the mounting hole, and the vibration end of the vibration structure abuts against the sample injection needle. Wherein the vibration end of the vibration structure impacts the top end of the sample injection needle, and the bottom end of the sample injection needle amplifies vibration and then vibrates and uniformly mixes a cell solution in the test tube. The uniform mixing of the cell solution in the test tube is more friendly, and the problems of cell hanging on the wall, low cell survival rate and non-uniform distribution of cells in the solution caused by uniform mixing of the cell solution in the test tube through rotation of the motor in the prior art are solved.

Description

Technical Field

[0001] The invention relates to the technical field of cytometers, and in particular to a cell solution mixing structure. Background Art

[0002] A flow cytometer is a device that automatically analyzes and sorts cells. It usually includes a cell solution mixing structure to mix the cell solution. The cell solution mixing structure usually includes at least a feed chamber, a test tube, and a motor rotating mixing structure. The feed chamber is used to insert a liquid injection needle, and the bottom of the liquid injection needle is inserted into the test tube to extract the cell solution in the test tube or add cell solution to the test tube; the test tube is set on the motor rotating mixing structure, and the motor rotating mixing structure is used to mix the cell solution inside the test tube after adding the cell solution to the test tube.

[0003] In the prior art, when the motor rotating mixing structure mixes the cell solution in the test tube, the cell solution in the test tube is prone to problems such as cell adhesion to the wall, low cell survival rate and uneven distribution of cells in the solution. In addition, since the shaft of the motor rotating mixing structure needs to rotate, the sealing problem of the motor shaft is also required.

[0004] Based on this, a new technical solution is needed. Summary of the invention

[0005] In view of this, an embodiment of the present invention provides a cell solution mixing structure to at least solve the shortcomings of the existing cell solution mixing structure using a motor to rotate the mixing structure.

[0006] The embodiment of the present invention provides the following technical solutions:

[0007] The embodiment of the present invention provides a cell solution mixing structure, comprising a feeding chamber and a test tube, wherein the feeding chamber comprises an injection needle through hole, an injection needle, and a mounting hole, wherein the injection needle through hole is arranged on the feeding chamber along the vertical direction of the feeding chamber, the injection needle penetrates and is inserted into the injection needle through hole, the top of the injection needle is limited and fixed in the injection needle through hole through a sealing joint, the bottom of the injection needle extends out of the injection needle through hole and is inserted into the test tube, and the mounting hole is opened on the feeding chamber along the horizontal direction of the feeding chamber and is communicated with the injection needle through hole;

[0008] The cell solution mixing structure also includes:

[0009] A vibration structure, wherein the vibration structure is disposed in the mounting hole, and a vibration end of the vibration structure abuts against the injection needle;

[0010] Wherein, when the vibration structure is turned on, the vibration end of the vibration structure hits the top end of the injection needle, and the bottom end of the injection needle amplifies the vibration to vibrate and mix the cell solution in the test tube.

[0011] Furthermore, the vibration structure comprises:

[0012] A base, the base being arranged at an end of the mounting hole away from the injection needle through hole;

[0013] A stacked piezoelectric ceramic, wherein the stacked piezoelectric ceramic is disposed in the mounting hole and is located between the base and the injection needle through hole, and an end of the stacked piezoelectric ceramic away from the injection needle through hole is limited by the base, and the stacked piezoelectric ceramic can undergo mechanical deformation and recovery in the direction of the injection needle through hole through the inverse piezoelectric effect;

[0014] A vibrating top block, which is slidably inserted into the mounting hole and is located between the stacked piezoelectric ceramics and the injection needle through hole, and is used to move in the mounting hole under the mechanical deformation of the stacked piezoelectric ceramics to push the injection needle to vibrate;

[0015] A spring, two ends of which are respectively connected to the vibration top block and the base, and the spring is used to pull the vibration top block to always be in contact with the stacked piezoelectric ceramics.

[0016] Furthermore, the vibration structure further comprises:

[0017] An adjusting screw is threadedly disposed on the base and abuts against an end of the stacked piezoelectric ceramic away from the vibrating top block, and is used for adjusting the positions of the stacked piezoelectric ceramic and the vibrating top block.

[0018] Furthermore, the vibrating top block comprises:

[0019] A top block groove, the top block groove is opened at one end of the vibration top block close to the stacked piezoelectric ceramics, and is used to install one end of the stacked piezoelectric ceramics close to the vibration top block;

[0020] The stacked piezoelectric ceramic comprises:

[0021] A limiting portion, the limiting portion is arranged on the outer wall of the stacked piezoelectric ceramics and is located at an end of the stacked piezoelectric ceramics away from the vibration top block;

[0022] Wherein, when the end face of the stacked piezoelectric ceramic close to the vibrating top block abuts against the bottom of the top block groove, the limiting portion abuts against the edge of the groove of the top block or has a preset interval, and the limiting portion is used to prevent the vibrating top block from over-pressing the stacked piezoelectric ceramic under the action of the spring.

[0023] Furthermore, the mounting hole comprises:

[0024] A first sub-mounting hole, which is opened on the feed cavity and communicates with the injection needle through hole;

[0025] A second sub-mounting hole, the second sub-mounting hole is opened on the feed cavity and is connected to the first sub-mounting hole, and the inner diameter of the second sub-mounting hole is larger than the inner diameter of the first sub-mounting hole;

[0026] Furthermore, the vibrating top block comprises:

[0027] A vibrating rod portion, the vibrating rod portion is sealed and slidably disposed in the first sub-mounting hole and is disposed corresponding to the injection needle;

[0028] A vibration base is connected to the vibration rod and is arranged in the second sub-mounting hole, and the top block groove is provided on a side of the vibration base away from the vibration rod.

[0029] Furthermore, the vibrating top block further comprises:

[0030] A sealing ring is sleeved on the vibration rod portion and is used for sealing between the vibration rod portion and the first sub-mounting hole.

[0031] Furthermore, a first connecting column is provided on the vibration top block, a second connecting column is provided on the base, and two ends of the spring are respectively connected to the first connecting column and the second connecting column.

[0032] Furthermore, the injection needle is a metal injection needle.

[0033] Furthermore, the cell solution mixing structure further comprises:

[0034] A test tube holder base is provided with a test tube holder, and the test tube holder and the test tube holder base are fully sealed.

[0035] Furthermore, the test tube support comprises:

[0036] The connecting part, the bottom end of the connecting part and the test tube holder base are fully sealed, and the connecting part is covered with a sealing ring.

[0037] Compared with the prior art, the at least one technical solution adopted in the embodiment of the present invention can achieve the following beneficial effects:

[0038] A cell solution mixing structure of the present invention uses a vibration structure to vibrate the injection needle, so as to amplify the vibration at the bottom of the injection needle and then mix the cell solution in the test tube, thereby solving the problems of cell wall adhesion, low cell survival rate and uneven distribution of cells in the solution caused by the rotation of a motor to mix the cell solution in the test tube in the prior art;

[0039] Furthermore, since a motor rotation structure is not required, the test tube holder base and the test tube holder in the cell solution mixing structure of the present application can be fully sealed to ensure the sealing of the cell solution mixing structure. BRIEF DESCRIPTION OF THE DRAWINGS

[0040] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0041] Figure 1 A cross-sectional view of a feed chamber portion in a cell solution mixing structure according to an embodiment of the present invention;

[0042] Figure 2 is a cross-sectional view of a vibration structure according to an embodiment of the present invention;

[0043] Figure 3 Schematic diagram of a test tube holder base, a test tube holder and a test tube according to an embodiment of the present invention.

[0044] 10. Feed cavity; 11. Sample injection needle through hole; 12. Sample injection needle; 13. Mounting hole; 131. First sub-mounting hole; 132. Second sub-mounting hole;

[0045] 20. Test tube;

[0046] 30. Vibration structure; 31. Base; 32. Stacked piezoelectric ceramics; 321. Positioning part; 33. Vibration top block; 331. Top block groove; 332. Vibration rod; 333. Vibration base; 334. Sealing ring; 34. Spring; 35. Adjusting screw; 36. First connecting column; 37. Second connecting column;

[0047] 40. Test tube support;

[0048] 50. Test tube support base. DETAILED DESCRIPTION

[0049] The embodiments of the present application are described in detail below with reference to the accompanying drawings.

[0050] The following describes the implementation methods of the present application through specific examples, and those skilled in the art can easily understand other advantages and effects of the present application from the contents disclosed in this specification. Obviously, the described embodiments are only a part of the embodiments of the present application, rather than all the embodiments. The present application can also be implemented or applied through other different specific implementation methods, and the details in this specification can also be modified or changed in various ways based on different viewpoints and applications without departing from the spirit of the present application. It should be noted that, in the absence of conflict, the following embodiments and the features in the embodiments can be combined with each other. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in the field without making creative work belong to the scope of protection of the present application.

[0051] It should be noted that various aspects of the embodiments within the scope of the appended claims are described below. It should be apparent that the aspects described herein can be embodied in a wide variety of forms, and any specific structure and / or function described herein is merely illustrative. Based on the present application, it should be understood by those skilled in the art that an aspect described herein can be implemented independently of any other aspect, and two or more of these aspects can be combined in various ways. For example, any number and aspect described herein can be used to implement the device and / or practice the method. In addition, other structures and / or functionalities other than one or more of the aspects described herein can be used to implement this device and / or practice this method.

[0052] It should also be noted that the illustrations provided in the following embodiments are only schematic illustrations of the basic concept of the present application. The drawings only show components related to the present application rather than being drawn according to the number, shape and size of components in actual implementation. In actual implementation, the type, quantity and proportion of each component may be changed arbitrarily, and the component layout may also be more complicated.

[0053] Additionally, in the following description, specific details are provided to facilitate a thorough understanding of the examples. However, it will be understood by those skilled in the art that the examples can be practiced without these specific details.

[0054] The existing cell solution mixing structure will cause problems such as uneven distribution of cells in the solution, low cell survival rate, cell wall adhesion, poor sealing, low injection pressure and slow injection speed after using a motor to rotate the mixing structure.

[0055] Based on this, this specification embodiment proposes a processing solution: Figures 1-2As shown, a cell solution mixing structure of the present application is provided with a vibration structure 30 on the feed chamber 10 to vibrate the injection needle 12, so that the bottom of the injection needle 12 amplifies the vibration and mixes the cell solution in the test tube 20, thereby eliminating the need for a motor to rotate the mixing structure and the need for the test tube 20 to rotate, thereby solving the problems of uneven distribution of cells in the solution, low cell survival rate, cell wall adhesion, poor sealing, low injection pressure and slow injection speed in the prior art.

[0056] The technical solutions provided by various embodiments of the present application are described below in conjunction with the accompanying drawings.

[0057] like Figures 1 to 3 As shown, a cell solution mixing structure of the present invention includes a feed chamber 10 and a test tube 20, the feed chamber 10 includes an injection needle through hole 11, an injection needle 12, and a mounting hole 13, the injection needle through hole 11 is arranged on the feed chamber 10 along the vertical direction of the feed chamber 10, the injection needle 12 penetrates and is inserted into the injection needle through hole 11, the top of the injection needle 12 is limited and fixed in the injection needle through hole 11 by a sealing joint, the bottom of the injection needle 12 extends out of the injection needle through hole 11 and is inserted into the test tube 20, and the mounting hole 13 is opened on the feed chamber 10 along the horizontal direction of the feed chamber 10 and is connected with the injection needle through hole 11.

[0058] Among them, the present application mainly improves the feed chamber 10, the test tube 20 and the base of the test tube 20 of the cell solution mixing structure, and other structural parts are similar or identical to the prior art.

[0059] The injection needle 12 is inserted into the test tube 20 to transport the cell solution into the test tube 20 or to extract the cell solution in the test tube 20 .

[0060] The size of the injection needle through hole 11 is slightly larger than the injection needle 12 , so as to facilitate the injection needle 12 to enter without affecting the subsequent vibration of the injection needle 12 .

[0061] The cell solution mixing structure also includes a vibration structure 30, which is arranged in the mounting hole 13, and the vibration end of the vibration structure 30 is in contact with the injection needle 12; wherein, when the vibration structure 30 is turned on, the vibration end of the vibration structure 30 hits the top of the injection needle 12, and the bottom end of the injection needle 12 amplifies the vibration to vibrate and mix the cell solution in the test tube 20.

[0062] The vibration structure 30 may be formed by a vibration motor, or other vibration structures 30 may be used.

[0063] In some embodiments, the vibration structure 30 includes a base 31 , a stacked piezoelectric ceramic 32 , a vibration top block 33 and a spring 34 . Among them, the base 31 is arranged at one end of the mounting hole 13 away from the injection needle through hole 11, and is used to provide mounting support; the stacked piezoelectric ceramic 32 is arranged in the mounting hole 13 and is located between the base 31 and the injection needle through hole 11, and the end of the stacked piezoelectric ceramic 32 away from the injection needle through hole 11 is limited by the base 31, and the stacked piezoelectric ceramic 32 can undergo mechanical deformation and recovery in the direction of the injection needle through hole 11 through the inverse piezoelectric effect; the vibrating top block 33 can be slidably inserted into the mounting hole 13 and is located between the stacked piezoelectric ceramic 32 and the injection needle through hole 11, and the vibrating top block 33 is used to move in the mounting hole 13 under the mechanical deformation of the stacked piezoelectric ceramic 32 to push the injection needle 12 to vibrate; the two ends of the spring 34 are respectively connected to the vibrating top block 33 and the base 31, and the spring 34 is used to pull the vibrating top block 33 to always contact with the stacked piezoelectric ceramic 32.

[0064] The base 31 provides a mounting support function to support the stacked piezoelectric ceramics 32 and the vibrating top block 33 , and to mount the other end of the spring 34 .

[0065] Among them, piezoelectric ceramics will produce mechanical deformation when undergoing the inverse piezoelectric effect.

[0066] The stacked piezoelectric ceramic 32 is formed by stacking a plurality of piezoelectric ceramics to increase the degree of mechanical deformation.

[0067] Specifically, when an external current is applied to the stacked piezoelectric ceramic 32, the stacked piezoelectric ceramic 32 can deform outward. Since the end of the stacked piezoelectric ceramic 32 away from the injection needle through hole 11 is supported by the base 31, the stacked piezoelectric ceramic 32 will deform in the direction of the injection needle through hole 11 to push the vibrating top block 33 to move.

[0068] Among them, after the vibrating top block 33 is pushed by the stacked piezoelectric ceramics 32, the vibrating top block 33 can push the sampling needle 12; after the stacked piezoelectric ceramics 32 recover, the vibrating top block 33 will be reset under the action of the spring 34, so that after the stacked piezoelectric ceramics 32 are repeatedly deformed and recovered, the vibrating top block 33 will repeatedly hit the sampling needle 12 to make the sampling needle 12 vibrate. Since the vibrating top block 33 makes the top end of the sampling needle 12 vibrate, the vibration will gradually amplify after being transmitted to the bottom end of the sampling needle 12, so as to achieve vibration mixing / stirring mixing of the cell solution in the test tube 20.

[0069] The spring 34 is used to pull the vibration top block 33 when the stacked piezoelectric ceramic 32 is restored so that the vibration top block 33 is always in contact with the stacked piezoelectric ceramic 32, so as to push the vibration top block 33 to move when the stacked piezoelectric ceramic 32 is deformed next time.

[0070] Furthermore, the vibration structure 30 also includes an adjusting screw 35 , which is threadedly disposed on the base 31 and abuts against one end of the stacked piezoelectric ceramics 32 away from the vibration top block 33 , for adjusting the positions of the stacked piezoelectric ceramics 32 and the vibration top block 33 .

[0071] Among them, rotating the adjusting screw 35 can reduce the space inside the mounting hole 13 and push the stacked piezoelectric ceramics 32 to move toward the side of the injection needle through hole 11, and then the stacked piezoelectric ceramics 32 push the vibrating top block 33 to move closer to the injection needle 12; rotating the adjusting screw 35 can increase the space inside the mounting hole 13, at this time, the vibrating top block 33 and the stacked piezoelectric ceramics 32 are away from the injection needle 12 under the action of the spring 34.

[0072] The vibrating top block 33 includes a top block groove 331 . The top block groove 331 is opened at one end of the vibrating top block 33 close to the stacked piezoelectric ceramics 32 and is used for mounting one end of the stacked piezoelectric ceramics 32 close to the vibrating top block 33 .

[0073] The stacked piezoelectric ceramic 32 includes a limiting portion 321, which is arranged on the outer wall of the stacked piezoelectric ceramic 32 and is located at one end of the stacked piezoelectric ceramic 32 away from the vibrating top block 33; wherein, when the end face of the stacked piezoelectric ceramic 32 close to the vibrating top block 33 abuts against the bottom of the top block groove 331, the limiting portion 321 abuts against the edge of the groove of the top block groove 331 or has a preset interval, and the limiting portion 321 is used to prevent the vibrating top block 33 from over-pressing the stacked piezoelectric ceramic 32 under the action of the spring 34.

[0074] The cooperation between the limiting portion 321 and the top block groove 331 can prevent the vibration top block 33 from over-pressing the stacked piezoelectric ceramics 32 under the action of the spring 34 , so that the stacked piezoelectric ceramics 32 produce a piezoelectric effect.

[0075] Furthermore, the mounting hole 13 includes a first sub-mounting hole 131 and a second sub-mounting hole 132. The first sub-mounting hole 131 is opened on the feed cavity 10 and communicates with the injection needle through hole 11; the second sub-mounting hole 132 is opened on the feed cavity 10 and communicates with the first sub-mounting hole 131, and the inner diameter of the second sub-mounting hole 132 is greater than the inner diameter of the first sub-mounting hole 131.

[0076] The vibrating top block 33 includes a vibrating rod 332 and a vibrating base 333. The vibrating rod 332 is sealed and slidably disposed in the first sub-mounting hole 131 and is disposed corresponding to the injection needle 12; the vibrating base 333 is connected to the vibrating rod 332 and is disposed in the second sub-mounting hole 132, and a top block groove 331 is provided on one side of the vibrating base 333 away from the vibrating rod 332.

[0077] The outer diameter of the vibration rod 332 is smaller than the outer diameter of the vibration base 333 , so as to facilitate the matching with the size of the injection needle 12 , and reduce the size and weight of the vibration top block 33 , so as to facilitate the vibration of the vibration top block 33 .

[0078] Furthermore, the vibration top block 33 further includes a sealing ring 334 , which is sleeved on the vibration rod portion 332 and is used to seal between the vibration rod portion 332 and the first sub-mounting hole 131 .

[0079] The sealing ring 334 is used to seal the injection needle through hole 11 to ensure the injection pressure.

[0080] Specifically, when the vibration rod 332 enters the injection needle through hole 11 to vibrate the injection needle 12, the sealing ring 334 is squeezed and deformed, which can provide good sealing, thereby increasing the injection pressure and the injection speed.

[0081] In some embodiments, the vibration top block 33 also includes a vibration cylinder portion, which is arranged on a side of the vibration base 333 away from the vibration rod portion 332. The vibration cylinder portion abuts against the base 31 under the action of the spring 34, and the inner diameter of the vibration cylinder portion is larger than the inner diameter of the vibration groove. The vibration cylinder portion is used to place the stacked piezoelectric ceramics 32 and the limiting portion 321.

[0082] The stacked piezoelectric ceramics 32 and the limiting portion 321 can slide in the vibration cylinder.

[0083] Furthermore, a first connecting column 36 is provided on the vibrating top block 33 , a second connecting column 37 is provided on the base 31 , and two ends of the spring 34 are connected to the first connecting column 36 and the second connecting column 37 respectively.

[0084] The first connecting column 36 may be disposed on the vibration cylinder.

[0085] Specifically, a through hole is provided on the side wall of the vibration cylinder, and the first connecting column 36 is disposed in the through hole. After the spring 34 is disposed in the through hole, it is connected to the first connecting column 36 .

[0086] Furthermore, the injection needle 12 is a metal injection needle 12 so as to maintain the functional integrity of the injection needle 12 when the injection needle 12 vibrates.

[0087] Furthermore, the cell solution mixing structure also includes a test tube holder base 50, on which a test tube holder 40 is arranged, and the test tube holder 40 and the test tube holder base 50 are fully sealed, thereby avoiding leakage between the test tube holder base 50 and the test tube holder 40, and solving the problem that the rotating shaft of the motor of the motor mixing structure and the test tube holder base 50 cannot be sealed.

[0088] The test tube holder 40 includes a connecting portion, the bottom end of which is fully sealed with the test tube holder base 50 , and a sealing ring 334 is disposed on the outer sleeve of the connecting portion, which can be sealed with the feed chamber 10 .

[0089] The working principle of the present invention is as follows:

[0090] When the cell solution in the test tube 20 is vibrated and mixed, current is applied to the stacked piezoelectric ceramics 32, and the stacked piezoelectric ceramics 32 will produce mechanical deformation due to the inverse piezoelectric effect, thereby pushing the vibrating top block 33 to move toward the injection needle 12 to push the injection needle 12 to vibrate;

[0091] After the stacked piezoelectric ceramics 32 are powered off and restored, the vibrating top block 33 is reset under the action of the spring 34 and always keeps in contact with the stacked piezoelectric ceramics 32, so that the stacked piezoelectric ceramics 32 continue to push the vibrating top block 33 to impact the injection needle 12 in the next cycle.

[0092] The vibration structure 30 of the present invention is integrally mounted on the feed chamber 10, and the vibration top block 33 of the vibration structure 30 touches the top of the metal injection needle 12, and the metal injection needle 12 undergoes elastic deformation and vibrates, so that the bottom of the injection needle 12 will amplify the vibration effect to mix the liquid in the test tube 20. In order to ensure the mixing effect, different input voltages are tested on the piezoelectric ceramic according to the characteristics of different liquids (such as viscosity, concentration, etc.), and the piezoelectric ceramic will output different amplitude values.

[0093] Compared with the prior art, the test tube holder 40 and the test tube holder base 50 of the present invention are fully sealed structures, and there is no need to consider the sealing problem at the motor rotating shaft. The structure is simpler, maintenance is more convenient, and the overall sealing of the loss cytometer is improved.

[0094] The cell solution mixing structure of the present invention has the following advantages:

[0095] 1. Simple structure and convenient operation, which improves the stability and maintainability of the equipment.

[0096] 2. High cell survival rate, reducing cell adhesion.

[0097] 3. High cell survival rate, reducing cell adhesion.

[0098] 4. Increase the injection pressure and injection speed.

[0099] 5. High degree of modularity and lower cost.

[0100] 6. The control method is simple, the equipment is highly stable, has a wide range of uses, and is easy to maintain.

[0101] In this specification, the same or similar parts between the various embodiments can be referred to each other, and each embodiment focuses on the differences from other embodiments. In particular, for the product embodiment described later, since it corresponds to the method, the description is relatively simple, and the relevant parts can be referred to the partial description of the system embodiment.

[0102] The above is only a specific implementation of the present application, but the protection scope of the present application is not limited thereto. Any changes or substitutions that can be easily thought of by a person skilled in the art within the technical scope disclosed in the present application should be included in the protection scope of the present application. Therefore, the protection scope of the present application shall be based on the protection scope of the claims.

Claims

1. A cell solution mixing structure, comprising a feeding chamber and a test tube, characterized in that: The feed cavity comprises a sample injection needle through hole, a sample injection needle, and a mounting hole. The sample injection needle through hole is arranged on the feed cavity along the vertical direction of the feed cavity. The sample injection needle penetrates and is inserted into the sample injection needle through hole. The top of the sample injection needle is limited and fixed in the sample injection needle through hole through a sealing joint. The bottom of the sample injection needle extends out of the sample injection needle through hole and is inserted into the test tube. The mounting hole is opened on the feed cavity along the horizontal direction of the feed cavity and is communicated with the sample injection needle through hole. The cell solution mixing structure also includes: A vibration structure, wherein the vibration structure is disposed in the mounting hole, and a vibration end of the vibration structure abuts against the injection needle; Wherein, when the vibration structure is turned on, the vibration end of the vibration structure hits the top end of the injection needle, and the bottom end of the injection needle amplifies the vibration to vibrate and mix the cell solution in the test tube.

2. The cell solution mixing structure according to claim 1, characterized in that: The vibration structure comprises: A base, the base being arranged at an end of the mounting hole away from the injection needle through hole; A stacked piezoelectric ceramic, wherein the stacked piezoelectric ceramic is disposed in the mounting hole and is located between the base and the injection needle through hole, and an end of the stacked piezoelectric ceramic away from the injection needle through hole is limited by the base, and the stacked piezoelectric ceramic can undergo mechanical deformation and recovery in the direction of the injection needle through hole through the inverse piezoelectric effect; A vibrating top block, which is slidably inserted into the mounting hole and is located between the stacked piezoelectric ceramics and the injection needle through hole, and is used to move in the mounting hole under the mechanical deformation of the stacked piezoelectric ceramics to push the injection needle to vibrate; A spring, two ends of which are respectively connected to the vibration top block and the base, and the spring is used to pull the vibration top block to always be in contact with the stacked piezoelectric ceramics.

3. The cell solution mixing structure according to claim 2, characterized in that: The vibration structure also includes: An adjusting screw is threadedly disposed on the base and abuts against an end of the stacked piezoelectric ceramic away from the vibrating top block, and is used for adjusting the positions of the stacked piezoelectric ceramic and the vibrating top block.

4. The cell solution mixing structure according to claim 3, characterized in that: The vibrating top block comprises: A top block groove, the top block groove is opened at one end of the vibration top block close to the stacked piezoelectric ceramics, and is used to install one end of the stacked piezoelectric ceramics close to the vibration top block; The stacked piezoelectric ceramic comprises: A limiting portion, the limiting portion is arranged on the outer wall of the stacked piezoelectric ceramics and is located at an end of the stacked piezoelectric ceramics away from the vibration top block; Wherein, when the end face of the stacked piezoelectric ceramic close to the vibrating top block abuts against the bottom of the top block groove, the limiting portion abuts against the edge of the groove of the top block or has a preset interval, and the limiting portion is used to prevent the vibrating top block from over-pressing the stacked piezoelectric ceramic under the action of the spring.

5. The cell solution mixing structure according to claim 4, characterized in that: The mounting hole comprises: A first sub-mounting hole, which is opened on the feed cavity and communicates with the injection needle through hole; A second sub-mounting hole, the second sub-mounting hole is opened on the feed cavity and is connected to the first sub-mounting hole, and the inner diameter of the second sub-mounting hole is larger than the inner diameter of the first sub-mounting hole; The vibrating top block comprises: A vibrating rod portion, the vibrating rod portion is sealed and slidably disposed in the first sub-mounting hole and is disposed corresponding to the injection needle; A vibration base is connected to the vibration rod and is arranged in the second sub-mounting hole, and the top block groove is provided on a side of the vibration base away from the vibration rod.

6. The cell solution mixing structure according to claim 5, characterized in that: The vibrating top block also includes: A sealing ring is sleeved on the vibration rod portion and is used for sealing between the vibration rod portion and the first sub-mounting hole.

7. The cell solution mixing structure according to claim 6, characterized in that: The vibrating top block is provided with a first connecting column, the base is provided with a second connecting column, and both ends of the spring are respectively connected to the first connecting column and the second connecting column.

8. The cell solution mixing structure according to claim 1, characterized in that: The injection needle is a metal injection needle.

9. The cell solution mixing structure according to any one of claims 1 to 8, characterized in that: Also includes: A test tube holder base is provided with a test tube holder, and the test tube holder and the test tube holder base are fully sealed.

10. The cell solution mixing structure according to claim 9, characterized in that: The test tube support comprises: The connecting part, the bottom end of the connecting part and the test tube holder base are fully sealed, and the connecting part is covered with a sealing ring.

Images