Mutton sheep unhairing combination agent as well as use method and application thereof

Abstract

The invention discloses a mutton sheep unhairing combination agent and a using method and application thereof, and belongs to the technical field of animal unhairing. In order to solve the technical problems that in the prior art, a shearing mode is low in efficiency and high in cost, trauma, discomfort and stress reaction are prone to being generated, and economic benefits of farmers are low, the mutton sheep unhairing combination agent is provided and comprises a traditional Chinese medicine compound and recombinant human epidermal growth factors, the traditional Chinese medicine compound comprises cyclophosphamide, licorice powder and borneol. According to the mutton sheep unhairing combination agent, time and labor are saved, the unhairing cost is reduced, the method is convenient to operate, strong discomfort, stress reaction and trauma cannot be caused to mutton sheep, and the mutton sheep unhairing combination agent can be applied to mutton sheep unhairing.

Description

Technical Field

[0001] The invention belongs to the technical field of animal depilation, and relates to a depilatory composition and a use method and application thereof. Background Art

[0002] At present, the traditional shearing methods are divided into manual shearing and mechanical shearing. Manual shearing has the disadvantages of long time, low efficiency, high cost, and easy trauma to meat sheep. In addition, in summer, due to the long duration of shearing, if the sheep are not sheared in time, it is easy to cause heat stress, which in turn affects the growth of meat sheep. Although mechanical shearing overcomes the shortcomings of manual shearing, such as long time, low efficiency, and easy trauma to meat sheep, it is still unsatisfactory due to various constraints such as mechanical performance, spare parts supply, and shearing technology. In addition, whether it is manual shearing or mechanical shearing, the shearing process involves operations such as capturing, isolating, bundling, and shearing, and the above operations will cause strong discomfort and stress reactions in meat sheep.

[0003] Therefore, technicians in this field are eager to develop an efficient, low-cost, and convenient mutton sheep depilation technology to reduce wool harvesting costs, improve the economic benefits of farmers, and provide technical support for promoting the development of the sheep industry. Summary of the invention

[0004] The present invention aims to solve the technical problems that the shearing methods in the prior art are low in efficiency, high in cost, easy to cause trauma, discomfort and stress reaction, and low economic benefits for farmers, and provides a mutton sheep depilatory composition and a use method and application thereof.

[0005] One of the purposes of the present invention is to provide a depilatory composition for mutton sheep, which comprises a Chinese medicine compound and recombinant human epidermal growth factor, wherein the Chinese medicine compound comprises cyclophosphamide, liquorice powder and borneol.

[0006] In a preferred embodiment of the present invention, the mass percentage of cyclophosphamide in the traditional Chinese medicine complex is 75-80%, the mass percentage of liquorice powder is 15-20%, and the mass percentage of borneol is 5%-10%.

[0007] The second object of the present invention is to provide a method for using the above-mentioned mutton depilatory composition, which comprises the following steps: administering the Chinese medicine complex to mutton sheep fed for 50 days, administering recombinant human epidermal growth factor 24 hours after the administration of the Chinese medicine complex, and administering the Chinese medicine complex and recombinant human epidermal growth factor once each during the mutton sheep feeding process, without the need for daily administration.

[0008] In a preferred embodiment of the present invention, the dosage of the Chinese medicine complex is: 27 mg / kg per mutton sheep body weight.

[0009] In a preferred embodiment of the present invention, the Chinese medicine complex is administered orally.

[0010] In a preferred embodiment of the present invention, the dosage of the recombinant human epidermal growth factor is: 120 μg / kg of the body weight of the mutton sheep.

[0011] In a preferred embodiment of the present invention, the administration method of the recombinant human epidermal growth factor is subcutaneous injection.

[0012] The third object of the present invention is to provide the use of the above-mentioned mutton sheep depilatory composition in mutton sheep depilation.

[0013] Beneficial effects of the present invention: The present invention provides a depilatory composition for mutton sheep, and a method for using and application thereof, wherein the depilatory composition for mutton sheep comprises: a Chinese medicine compound and a recombinant human epidermal growth factor, and the Chinese medicine compound comprises: cyclophosphamide, licorice powder and borneol. Compared with the traditional depilatory method, the depilatory composition for mutton sheep provided by the present invention saves time and manpower, reduces the cost of depilation, and the method is easy to operate and does not cause strong discomfort, stress reaction and trauma to mutton sheep; the depilatory composition for mutton sheep provided by the present invention causes the wool to fall off naturally 11-13 days after administration, and the wool begins to grow naturally after 45 days, and has no significant adverse effect on the growth of mutton sheep; compared with the depilatory effects of mutton sheep with other cyclophosphamide drugs and epidermal growth factor administered alone, the depilatory time is shortened and the effect is better. The Chinese medicine complex of the present invention acts on the wool follicle fold cells of meat sheep through cyclophosphamide, inducing cell apoptosis and promoting hair loss; at the same time, it is combined with human epidermal growth factor to bind to receptors on hair root sheath cells, causing changes in the generation and secretion activities of outer hair root sheath cells, thereby causing hair follicles to stop growing and enter a regression period. The two interact with each other, thereby achieving the purpose of efficient, low-cost and convenient hair removal, reducing wool harvesting costs, improving the economic benefits of farmers, and providing technical support for promoting the development of the sheep industry. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 This is the depilation effect diagram of the control group of sheep;

[0015] Figure 2 The depilatory effect diagram of the depilatory composition prepared in Example 1. DETAILED DESCRIPTION

[0016] Those skilled in the art can refer to the content of this article and appropriately improve the process parameters. It should be particularly noted that all similar replacements and modifications are obvious to those skilled in the art, and they are all considered to be included in the present invention. The method and application of the present invention have been described through preferred embodiments, and relevant personnel can obviously modify or appropriately change and combine the methods and applications described herein without departing from the content and scope of the present invention to implement and apply the technology of the present invention.

[0017] In order to make the purpose, technical scheme and advantages of the present invention clearer, the present invention is further described in detail below in conjunction with specific embodiments. The test methods used in the following examples are all conventional methods unless otherwise specified, and the materials, reagents, methods and instruments used are conventional materials, reagents, methods and instruments in the art unless otherwise specified, and those skilled in the art can obtain them through commercial channels.

[0018] Embodiment 1:

[0019] In this embodiment, cyclophosphamide, licorice powder and borneol are mixed in a certain proportion to prepare a Chinese medicine complex, which includes the following components: cyclophosphamide by mass percentage of 80%, licorice powder by mass percentage of 15%, and borneol by mass percentage of 5%; and recombinant human epidermal growth factor is administered at the same time. The Chinese medicine complex is orally administered at a weight of 27 mg / kg for mutton sheep, and the recombinant human epidermal growth factor is administered subcutaneously at a weight of 120 μg / kg for mutton sheep.

[0020] The Chinese medicine complex prepared in this example was orally administered to the sheep at 27 mg / kg of the sheep's body weight. 24 hours after the administration of the Chinese medicine complex, the recombinant human epidermal growth factor was subcutaneously injected at 120 μg / kg of the sheep's body weight.

[0021] Embodiment 2:

[0022] In this embodiment, cyclophosphamide, licorice powder and borneol are mixed in a certain proportion to prepare a Chinese medicine complex, which includes the following components: cyclophosphamide by mass percentage of 75%, licorice powder by mass percentage of 20%, and borneol by mass percentage of 5%; and recombinant human epidermal growth factor is administered at the same time. The Chinese medicine complex is orally administered at a weight of 27 mg / kg for mutton sheep, and the recombinant human epidermal growth factor is administered subcutaneously at a weight of 120 μg / kg for mutton sheep.

[0023] The Chinese medicine complex prepared in this example was orally administered to the sheep at 27 mg / kg of the sheep's body weight. 24 hours after the administration of the Chinese medicine complex, the recombinant human epidermal growth factor was subcutaneously injected at 120 μg / kg of the sheep's body weight.

[0024] Embodiment 3:

[0025] In this embodiment, cyclophosphamide, licorice powder and borneol are mixed in a certain proportion to prepare a Chinese medicine complex, which includes the following components: cyclophosphamide by mass percentage of 77%, licorice powder by mass percentage of 17%, and borneol by mass percentage of 6%; and recombinant human epidermal growth factor is administered at the same time. The Chinese medicine complex is orally administered at a weight of 27 mg / kg for mutton sheep, and the recombinant human epidermal growth factor is administered subcutaneously at a weight of 120 μg / kg for mutton sheep.

[0026] The Chinese medicine complex prepared in this example was orally administered to the sheep at 27 mg / kg of the sheep's body weight. 24 hours after the administration of the Chinese medicine complex, the recombinant human epidermal growth factor was subcutaneously injected at 120 μg / kg of the sheep's body weight.

[0027] Comparative Example 1:

[0028] In this example, only cyclophosphamide was administered to the test subjects, and the oral administration was 27 mg / kg of the body weight of the mutton sheep.

[0029] Comparative Example 2:

[0030] In this example, only recombinant human epidermal growth factor was administered to the test subjects, and the administration was performed by subcutaneous injection at a dose of 120 μg / kg of the sheep's body weight.

[0031] Effect test:

[0032] Test location: Heilongjiang Woyuan Animal Husbandry Co., Ltd.

[0033] Experimental livestock: Hu sheep

[0034] Experimental plan: 400 Hu sheep with the same body weight, age and other physical conditions were selected and divided into experimental group 1, experimental group 2, experimental group 3 and control group, with 100 Hu sheep in each group. The Hu sheep were given the Chinese medicine compound and recombinant human epidermal growth factor once each. The Hu sheep in the control group were injected with the same volume of saline, and the Hu sheep in experimental groups 1-3 and the control group were guaranteed to have sufficient drinking water and food.

[0035] Result data:

[0036] In this example, the growth performance of the Hu sheep in Examples 1-3 and the control group was tested to explore the effect of the depilatory composition on the growth of the Hu sheep. The results are shown in Table 1. It can be seen that there is no significant difference in the average daily feed intake between the Hu sheep in the control group and the Hu sheep in Examples 1-3, indicating that the use of the depilatory composition has no effect on the feeding of the Hu sheep; under the premise that the difference in the average daily feed intake is not significant, the average daily weight gain of the Hu sheep in Examples 1-3 is slightly higher than that in the control group, and the feed-to-weight ratio F / G is slightly lower than that in the control group, indicating that the use of the depilatory composition has no effect on the growth of the Hu sheep.

[0037] The depilatory effects of the Hu sheep in Example 1 and the control group are as follows Figure 1-2 As shown, it can be seen that after using the depilatory composition in Example 1, the wool of the Hu sheep falls off naturally without causing external injuries to the Hu sheep. The depilatory effect is good and the safety is high.

[0038] Table 1

[0039] Group Average daily weight gain Average daily feed intake Material weight ratio F / G Control group <![CDATA[209.57±24.39 a ]]> <![CDATA[1.64±0.14 a ]]> 7.85±0.61 Example 1 <![CDATA[211.34±16.34 a ]]> <![CDATA[1.65±0.19 a ]]> 7.8±1.13 Example 2 <![CDATA[215.44±20.56 a ]]> <![CDATA[1.66±0.17 a ]]> 7.71±1.17 Example 3 <![CDATA[210.58±19.34 a ]]> <![CDATA[1.65±0.18 a ]]> 7.84±1.16

[0040] Data in the same column with different letters on the shoulders indicate significant differences (P < 0.05); data with the same letters on the shoulders or no letters on the shoulders indicate no significant differences (P > 0.05).

[0041] This example is to explore the depilatory effect of the depilatory composition on Hu sheep, and the depilatory effect of Hu sheep in Examples 1-3 and Comparative Examples 1-2 is statistically analyzed. The results are shown in Table 2. It can be seen that the depilatory composition prepared in Examples 1 and 3 began to depilate Hu sheep 11 days after administration, which was 5 days less than Comparative Example 1 and 6 days less than Comparative Example 2. The depilatory composition prepared in Example 2 began to depilate Hu sheep 13 days after administration, which was 3 days less than Comparative Example 1 and 4 days less than Comparative Example 2. It shows that the depilatory composition provided in Examples 1-3 of the present invention depilates with the fastest efficiency under the premise of ensuring safety, growth and performance, and the depilatory effect is better. The wool of the mutton depilatory composition provided by the present invention begins to fall off naturally 11-13 days after administration, and the wool begins to grow naturally after 45 days.

[0042] Table 2

[0043] Group Hair removal days Example 1 11 Example 2 13 Example 3 11 Comparative Example 1 16 Comparative Example 2 17

[0044] The contents not described in detail in the specification of the present invention are well-known technologies for those skilled in the art. Although the present invention has been disclosed as above with preferred embodiments, it is not intended to limit the present invention. Anyone familiar with this technology can make various changes and modifications without departing from the spirit and scope of the present invention. Therefore, the protection scope of the present invention should be based on the definition of the claims.

Claims

1. A depilatory composition for mutton sheep, characterized in that: The depilatory combination for mutton sheep comprises: a traditional Chinese medicine compound and recombinant human epidermal growth factor, and the traditional Chinese medicine compound comprises: cyclophosphamide, liquorice powder and borneol.

2. The depilatory composition for mutton sheep according to claim 1, characterized in that: The mass percentage of cyclophosphamide in the traditional Chinese medicine complex is 75-80%, the mass percentage of liquorice powder is 15-20%, and the mass percentage of borneol is 5%-10%.

3. The method for using the depilatory composition for mutton sheep according to any one of claims 1 to 2, characterized in that: The method of use comprises the following steps: administering the Chinese medicine compound to 50-day-old mutton sheep, administering the recombinant human epidermal growth factor 24 hours after the administration of the Chinese medicine compound, and administering the Chinese medicine compound and the recombinant human epidermal growth factor once each during the mutton sheep feeding process, without the need for daily administration.

4. The method of use according to claim 3, characterized in that: The dosage of the Chinese medicine complex is: 27 mg / kg based on the body weight of the mutton sheep.

5. The method of use according to claim 3, characterized in that: The administration method of the traditional Chinese medicine complex is oral administration.

6. The method of use according to claim 3, characterized in that: The dosage of the recombinant human epidermal growth factor is: 120 μg / kg according to the body weight of the mutton sheep.

7. The method of use according to claim 3, characterized in that: The administration method of the recombinant human epidermal growth factor is subcutaneous injection.

8. Use of the mutton sheep depilatory composition according to any one of claims 1 to 2 in depilating mutton sheep.

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