Three-phase separation device and bilirubin separation and extraction method

Abstract

The invention belongs to the technical field of bilirubin separation and extraction, and discloses a three-phase separation device and a bilirubin separation and extraction method.The device comprises an outer shell, the inner portion of the outer shell is a working cavity, the upper portion of the outer shell is provided with a cover capable of being opened and closed in a sealed mode, and a bottom opening of the outer shell is provided with a discharging port; the rotary drum barrel is coaxially and rotatably arranged in the working cavity, and the outer diameter of the rotary drum barrel is smaller than the inner diameter of the working cavity; the driving part is in transmission connection with the rotary drum barrel and drives the rotary drum barrel to rotate in the working cavity; wherein an opening is formed in the bottom of the rotary drum barrel; a feeding pipe is arranged on the cover body, and the feeding pipe can extend into the rotary drum barrel. According to the bilirubin separation and extraction method, solid and two-phase liquid are separated by using the device. According to the device and the method, flocs in materials are agglomerated into blocks, then the blocks are simply filtered, so that solid and two-phase liquid can be separated, the operation is simple and rapid, continuous production can be realized, and the extraction rate and the production efficiency are high.

Description

Technical Field

[0001] The invention belongs to the technical field of bilirubin separation and extraction, and specifically relates to a three-phase separation device and a bilirubin separation and extraction method. Background Art

[0002] The bilirubin production process involves separating and extracting bilirubin from the raw material liquid. Since bilirubin is insoluble in water and soluble in organic reagents, organic reagents are often used to extract and separate bilirubin in production. However, the raw material liquid is usually obtained by fermentation, which is relatively thick and has more flocculent impurities. The flocculent impurities are extremely small. When the bilirubin is extracted by organic reagents, the three phases of water, flocculent impurities and organic reagents are seriously emulsified and mixed into one. It is impossible to separate them by conventional filtration such as plate and frame filtration, three-legged centrifugation, disc centrifugation and other equipment. The three-phase separation is extremely difficult. The prior art usually uses conventional high-speed centrifuges for long-term centrifugation for separation, which has the problems of long separation time, insufficient separation degree, and more bilirubin remaining in the flocculent impurities; and the floccules are very easy to block the filter holes of the centrifuge, and the separation speed is slow; it is necessary to stop the centrifuge from time to time for cleaning and then centrifugation, and centrifugation can only be performed intermittently, which is difficult to automatically and continuously produce, and the production efficiency and extraction rate are both low. Summary of the invention

[0003] In view of the above-mentioned deficiencies in the prior art, the present invention provides a three-phase separation device and a method for separating and extracting bilirubin.

[0004] The technical solution adopted by the present invention is as follows: A three-phase separation device, comprising: An outer shell, wherein the interior of the outer shell is a working chamber, the upper portion is provided with a cover that can be opened and sealed, and the bottom opening is provided as a discharge port; A rotating drum is coaxially rotatably disposed in the working chamber, and has an outer diameter smaller than an inner diameter of the working chamber; A driving member, which is in driving connection with the rotary drum and drives the rotary drum to rotate in the working chamber; in, The bottom of the rotating drum is open; The cover body is provided with a feed pipe, and the feed pipe can extend into the rotating drum.

[0005] In one embodiment of the present application, a drum head is further included. The drum head is coaxially arranged in the working chamber and is fixedly connected to the drum tube via a first bracket. The drum head is connected to the driving member.

[0006] In one embodiment of the present application, the driving member includes a first driving motor, a transmission belt and a main shaft; The upper part of the main shaft is fixedly connected to the drum head, the middle part is rotatably connected to the second bracket via a bearing, and the lower part is transmission-connected to the output end of the first drive motor via the transmission belt; The second bracket is fixedly connected to the outer shell.

[0007] In one embodiment of the present application, the driving member includes a second driving motor, which is coaxially arranged at the lower part of the drum head and fixedly connected to the outer shell via a second bracket; the output end of the second driving motor is connected to the drum head.

[0008] In one embodiment of the present application, when the cover body is closed, the outlet of the feed pipe is arranged toward the rotation direction of the inner wall of the rotating drum.

[0009] In one embodiment of the present application, a circle of closing portion is provided at the bottom of the side wall of the rotating drum tube extending inward; the projection position of the outlet of the feed pipe is located within the projection area of ​​the closing portion.

[0010] In one embodiment of the present application, the side wall of the rotary drum has no filter holes; Alternatively, the side wall of the rotary drum has filter holes and is provided with an annular non-porous membrane bag adapted to the inner wall of the rotary drum. The non-porous membrane bag has openings at the top and bottom and can be detachably installed in the rotary drum.

[0011] In one embodiment of the present application, it further includes a first mounting frame and a second mounting frame, the outer shell is fixedly mounted on the first mounting frame, and the first mounting frame is suspendedly mounted on the second mounting frame via a buffer member.

[0012] In one embodiment of the present application, one or more of the following features are included: It also includes a material receiving container and a filter element, wherein the filter element is detachably mounted at the opening of the material receiving container and can be placed under the second mounting frame corresponding to the material outlet; It also includes a delivery pump, which is connected to the feed pipe via a hose; A discharge cylinder is installed at the discharge port; A flushing port is provided on the side wall of the outer shell, and the flushing port is connected to a water pipe.

[0013] A method for separating and extracting bilirubin, using the three-phase separation device as described in any one of the above, comprises the following steps: S1, adding water and an organic solvent to the bilirubin raw material solution, mixing them evenly, to obtain a mixed material; S2, closing the cover of the outer shell, starting the driving member to drive the drum to rotate at a certain speed; S3, sucking a certain volume of the mixed material by a delivery pump, pumping it into the drum through a feed pipe, and rotating and centrifuging it; S4, after a certain period of rotary centrifugal treatment, the rotating drum is controlled to reduce the speed or stop rotating, so that the material after rotary centrifugal treatment is discharged from the discharge port at the bottom; S5, starting or increasing the rotation speed of the drum to rotate the drum at a certain speed, and repeating steps S3 to S5 until the mixed material is processed; S6, the discharged material is separated into solid and liquid through a filter element, the separated liquid is allowed to stand for stratification, and the aqueous phase and the organic solvent are separated to obtain an organic solvent containing bilirubin.

[0014] Compared with the prior art, the present invention has the following beneficial effects: 1. The three-phase separation device of the present invention is provided with an opening at the bottom of the outer shell and the drum, and a feed pipe is provided on the cover body to extend into the drum, so that the mixed material is introduced into the drum when the drum rotates at a high speed, so that the material stays in the drum under the centrifugal force of the high-speed rotation, and the layers are separated from the outside to the inside in sequence into an organic reagent layer (such as chloroform, with a density of 1.48 g / cm 3 , greater than the density of flocs and water), floc layer and water layer; after high-speed rotation for a certain period of time, the flocs can be compacted into compact blocks under the action of centrifugal force and the extrusion of the organic phase and the aqueous phase, and then the drum is controlled to reduce the speed or stop rotating, and the compacted floc blocks, organic reagents and water are discharged from the discharge port at the bottom. The discharged material can be quickly separated from the flocs and the organic phase and the aqueous phase by simple filtration, and the separated organic phase and aqueous phase can be separated by static stratification, thereby realizing three-phase separation.

[0015] 2. After a certain amount of material is introduced into the three-phase separation device and subjected to high-speed centrifugal force treatment for a certain period of time, the speed is reduced or stopped to discharge the material from the bottom outlet, and the next round of feeding and high-speed rotation treatment can be carried out immediately. There is no need to clean the drum, etc., and automated and continuous production processing can be achieved with high production efficiency.

[0016] 3. The three-phase separation device is particularly suitable for the separation and extraction of bilirubin in viscous materials. Bilirubin is dissolved in an organic solvent (chloroform). After high-speed centrifugal force treatment, the flocculent impurities in the raw material liquid are compacted into blocks. Bilirubin is fully dissolved in the organic solvent. After filtering and static stratification treatment, the organic solvent containing bilirubin can be quickly and efficiently separated. The three-phase separation device is used for the separation and extraction of bilirubin. The bilirubin residue in the separated flocculent impurities is small, the extraction rate is high, and the treatment process is simple, fast and efficient. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0018] Figure 1 It is a schematic diagram of the overall structure of the three-phase separation device of the present invention.

[0019] Figure 2 It is a schematic diagram of the cover-opening structure of the three-phase separation device of the present invention.

[0020] Figure 3 It is a schematic diagram of the top view of the structure inside the outer shell.

[0021] Figure 4 It is a schematic diagram of the cross-sectional structure inside the outer shell.

[0022] Figure 5 It is a schematic diagram of the structure showing the drum head and drum tube in cross section.

[0023] Reference numerals: 1. Outer shell; 11. Cover body; 111. Feed pipe; 12. Discharge port; 121. Discharge barrel; 13. Flushing port; 2. Rotating drum; 21. Closing part; 3. driving member; 31. first driving motor; 32. transmission belt; 33. bearing member; 34. second bracket; 4. Drum head; 41. First bracket; 5. first mounting frame; 51. second mounting frame; 52. buffer member; 6. Material receiving container; 61. Filter element. DETAILED DESCRIPTION

[0024] In the following, only some exemplary embodiments are briefly described. As those skilled in the art will appreciate, the described embodiments may be modified in various ways without departing from the spirit or scope of the present invention. Therefore, the drawings and descriptions are considered to be exemplary and non-restrictive in nature.

[0025] In the description of the present invention, it should be understood that the terms "upper", "lower", "front", "rear", "vertical", "top", "bottom", "inner", "outer", "axial", "radial" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, or are the orientations or positional relationships in which the products of the present invention are conventionally placed when in use, or are the orientations or positional relationships conventionally understood by those skilled in the art. They are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present invention.

[0026] The terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, a feature defined as "first" or "second" may explicitly or implicitly include one or more of the features. In the description of the present invention, the meaning of "plurality" is two or more, unless otherwise clearly and specifically defined.

[0027] In the present invention, unless otherwise clearly specified and limited, the terms "installed", "connected", "connected", "fixed" and the like should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be directly connected or indirectly connected through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0028] In the present invention, unless otherwise clearly specified and limited, a first feature being "above" or "below" a second feature may include that the first and second features are in direct contact, or may include that the first and second features are not in direct contact but are in contact through another feature between them. Moreover, a first feature being "above", "above" and "above" a second feature includes that the first feature is directly above and obliquely above the second feature, or simply indicates that the first feature is higher in level than the second feature. A first feature being "below", "below" and "below" a second feature includes that the first feature is directly below and obliquely below the second feature, or simply indicates that the first feature is lower in level than the second feature.

[0029] The disclosure below provides many different embodiments or examples to realize different structures of the present invention. In order to simplify the disclosure of the present invention, the parts and settings of specific examples are described below. Of course, they are only examples, and the purpose is not to limit the present invention.

[0030] The embodiments of the present invention are described in detail below with reference to the accompanying drawings.

[0031] The embodiment of the present invention provides a three-phase separation device, which is suitable for solid-liquid separation of a three-phase mixed solution of a solid phase, an organic phase and an aqueous phase, and is particularly suitable for solid-liquid separation treatment of a dense flocculent mixture.

[0032] like Figures 1 to 5 As shown, the three-phase separation device includes an outer shell 1, a rotating drum 2, a driving member 3 and the like.

[0033] Specifically, the outer shell 1 is arranged vertically, and the interior is a working chamber, the upper part of the working chamber is open, and a cover 11 is provided, which can be opened and sealed; the bottom of the working chamber is open, and the bottom opening is provided as a discharge port 12. That is, the working chamber is through from top to bottom. The cover 11 is connected to a power member such as a cylinder or a telescopic rod, and the power member drives the cover 11 to close or open. During operation, the cover 11 covers the outer shell 1 and is connected and tightened through a number of connecting members provided around the upper opening of the outer shell 1.

[0034] The drum 2 is coaxially rotatable in the working chamber of the outer shell 1. The outer diameter of the drum 2 is slightly smaller than the inner diameter of the working chamber. The upper and lower parts of the drum 2 are completely located in the working chamber and are adapted to the working chamber.

[0035] The driving member 3 is in driving connection with the rotating drum 2 , and the driving member 3 drives the rotating drum 2 to rotate in the working chamber.

[0036] The bottom of the rotating drum 2 is open, and the bottom opening of the rotating drum 2 corresponds to the discharge port 12 of the outer shell 1 .

[0037] A feed pipe 111 is provided on the cover body 11 . When the cover body 11 is closed, the feed pipe 111 can extend into the drum 2 , and the material is transported into the drum 2 through the feed pipe 111 on the cover body 11 .

[0038] The working process of the three-phase separation device is as follows: the driving member 3 drives the drum 2 to rotate at a high speed (the "high speed" refers to the linear speed of the circumferential rotation of the drum 2), the mixed material is input into the rotating drum 2 through the feed pipe 111, and the mixed material is kept in the drum 2 and rotates at a high speed under the driving force of the high-speed rotating drum 2. Under the action of high-speed rotation and centrifugal force, the material is layered according to the specific gravity / density, the organic solvent with a larger specific gravity is located in the outermost layer, the flocculent impurities with a medium specific gravity are located in the middle layer, and the water with the smallest specific gravity is located in the innermost layer; after a certain period of high-speed rotation treatment, the flocculent impurities form a compact mass under the centrifugal force and the extrusion of the organic phase and the water phase, at this time, the rotation of the drum 2 can be reduced or stopped, and the material after high-speed rotation treatment is discharged from the discharge port 12 at the bottom; the discharged material can be quickly separated into solid and liquid by simple filtration. The separation operation is simple and fast, and the separation efficiency and extraction rate are high.

[0039] like Figures 3 to 5As shown, in one embodiment, the separation device further comprises a drum head 4 connecting the driving member 3 and the drum tube 2. The drum head 4 is coaxially arranged in the working chamber of the outer shell 1, located inside and / or below the drum tube 2; the outer diameter of the drum head 4 is smaller than the inner diameter of the drum tube 2. The drum head 4 is fixedly connected to the lower part of the drum tube 2 via a first bracket 41; the drum head 4 is connected to the driving member 3, and the driving member 3 drives the drum head 4 to rotate, thereby driving the drum tube 2 to rotate.

[0040] The first bracket 41 is preferably a plurality of connecting rods extending in the radial direction of the drum tube 2 / drum head 4 , one end of which is connected to the drum head 4 , and the other end is connected to the bottom of the drum tube 2 , and rotates with the drum head 4 and the drum tube 2 .

[0041] In one embodiment, Figures 1 to 4 As shown, the driving member 3 includes a first driving motor 31, a transmission belt 32 and a main shaft (not shown in the figure). The upper part of the main shaft is fixedly connected to the drum head 4, a bearing member 33 is installed in the middle part, and the second bracket 34 is rotatably connected to the bearing member 33, and the lower part is transmission-connected to the output end of the first driving motor 31 through the transmission belt 32. The second bracket 34 is fixedly connected to the outer shell 1, and is used to provide a rotation installation position for the drum head 4. The first driving motor 31 is arranged on one side outside the outer shell 1, so as to achieve a yield installation and facilitate inspection and maintenance.

[0042] Preferably, the second bracket 34 is also configured as a plurality of connecting rods arranged along the radial direction of the working chamber / discharge port 12, one end of which is connected to the outer shell 1, and the other end is connected to the bearing seat in the bearing member 33, and the setting position is fixed.

[0043] The first bracket 41 and the second bracket 34 are both configured as radially extending connecting rods, which occupy a smaller projection area of ​​the discharge port 12, so that the discharge port 12 of the working chamber is hollowed out and connected, ensuring that the material processed by high-speed rotation can fall smoothly from the discharge port 12 under the action of gravity.

[0044] In another embodiment, the driving member 3 includes a second driving motor (not shown in the figure), which is coaxially arranged at the lower part of the drum head 4 and is connected and fixed to the outer shell 1 via a second bracket 34 composed of multiple connecting rods; the output end of the second driving motor is connected to the drum head 4 to drive the drum head 4 to rotate. The projection area of ​​the second driving motor should be smaller than the projection area of ​​the discharge port 12 and the lower opening of the drum tube 2 to ensure that the material can fall smoothly from the bottom opening.

[0045] Preferably, when the cover body 11 is closed, the outlet of the feed pipe 111 is arranged toward the rotation direction of the inner wall of the drum 2, so that when the material enters, it impacts the inner wall of the drum 2 and rotates at high speed with the drum 2, avoiding splashing and disturbing the rotation of the material in the drum 2, so that the material is separated completely and the discharge of unextruded or incompletely extruded flocs is avoided.

[0046] like Figure 3 , Figure 4 and Figure 5 As shown, the bottom of the side wall of the drum 2 preferably extends inwardly to form a circle of closing portion 21. The closing portion 21 can prevent the material from being thrown out from the bottom during high-speed rotation, and at the same time increase the amount of material that the drum 2 can process and contain in one rotation, thereby improving the processing efficiency.

[0047] Furthermore, the closing portion 21 is tilted downward inwardly along the radial direction of the drum 2, and the tilt angle is preferably 5-30 degrees. The closing portion 21 is tilted downward, and the tilt angle is limited to ensure that the material is not thrown out from the bottom during the rotation process, and the material after the rotation process can slide down and be discharged.

[0048] Preferably, the projection position of the outlet of the feed pipe 111 is located within the projection area of ​​the closing portion 21. This can effectively prevent the input material from being thrown out from the bottom opening without being processed by high-speed rotation, and can well ensure that the material is completely processed by high-speed rotation and extrusion.

[0049] In one embodiment, the side wall of the drum 2 is provided with worry-free holes. The material is squeezed under the interaction of the centrifugal force and the side wall of the drum 2 and will not be thrown out through the side wall.

[0050] In another embodiment, the side wall of the drum 2 is provided with a plurality of filter holes. At the same time, the separation device is equipped with an annular non-porous membrane bag (not shown in the figure) adapted to the inner wall of the drum 2. The annular non-porous membrane bag is open at the top and bottom and can be detachably installed in the drum 2. When the separation device is used to treat a mixed solution to extrude solid impurities into blocks, the annular non-porous membrane bag is used for high-speed rotation processing; when the separation device is directly used for centrifugal filtration of materials, the annular non-porous membrane bag is not required, or a filter bag with only an upper opening is used for centrifugal filtration. The side wall of the drum 2 is provided with filter holes and is equipped with a detachable annular non-porous membrane bag, which can effectively expand the scope of use of the separation device and provide more usage scenarios.

[0051] Preferably, if Figure 1 and Figure 2 As shown, the separation device further includes a first mounting frame 5 and a second mounting frame 51. The outer shell 1 is fixedly mounted on the first mounting frame 5, the first mounting frame 5 is suspended on the second mounting frame 51 via a buffer 52, and the second mounting frame 51 is erected on the ground or a base. The two mounting frames are provided and connected via the buffer 52, which can effectively reduce the impact of vibration and impact on the device and reduce noise.

[0052] It also includes a material receiving container 6 and a filter 61, and the filter 61 is detachably mounted at the opening of the material receiving container 6. The material receiving container 6 can be a mobile liquid storage barrel or a fixed liquid storage tank. When the material receiving container 6 is a mobile liquid storage barrel, the material receiving container 6 can be placed under the second mounting frame 51 after the filter 61 is installed, and corresponds to the discharge port 12 at the bottom of the outer shell 1. When the material receiving container 6 is a fixed liquid storage tank, the filter 6 is detachably mounted between the discharge port 12 and the entrance of the material receiving container 6, which is convenient for replacement and cleaning.

[0053] The filter element 61 can be a filter frame or filter cloth with a pore size of 200 meshes. The filter element 61 can be detachably limited and placed at the opening of the receiving container 6, or can be detachably arranged between the discharge port 12 and the entrance of the receiving container 6. When the material processed by high-speed rotation is discharged from the discharge port 12, it can be directly filtered by the filter element 61 to achieve solid-liquid separation and quickly separate solid impurities.

[0054] A delivery pump (not shown) is also provided, which is mounted on the first mounting frame 51, and its outlet end is connected to the feed pipe 111 via a hose, and is used to pump the mixed material into the high-speed rotating drum 2 and control the amount of the pumped material.

[0055] Preferably, a discharge barrel 121 is installed at the discharge port 12 at the bottom of the outer shell 1, and the discharge barrel 121 covers the drum head 4 and other structures. The discharge barrel 121 is provided to guide the material to prevent the discharged material from splashing around, especially when the drum 2 rotates at a low speed.

[0056] In one embodiment, at least one flushing port 13 is provided on the side wall of the outer shell 1, and the flushing port 13 is connected to a water pipe. Water can be flushed between the outer wall of the drum 2 and the inner wall of the outer shell 1 through the flushing port 13 to clean the inner wall of the outer shell 1 and the drum 2.

[0057] Based on the same purpose of the invention, an embodiment of the present invention further provides a method for separating and extracting bilirubin, which uses the three-phase separation device described above for separation.

[0058] The bilirubin separation and extraction method comprises the following steps: S1, adding a small amount of water (or no water) and an appropriate amount of organic solvent to the raw material liquid of bilirubin, stirring and mixing them thoroughly to obtain a mixed material.

[0059] Among them, the organic solvent is preferably chloroform (i.e., chloroform). Bilirubin is insoluble in water but soluble in chloroform. More preferably, water is first added to disperse the thick raw material liquid, and then chloroform is added to dissolve the bilirubin. First, water is added to disperse, and then chloroform is added, and the mixture is stirred and mixed thoroughly to make the bilirubin in the thick raw material liquid fully dissolved in the organic solvent.

[0060] S2, close the cover 11 of the outer shell 1, start the driving member 3 to drive the drum 2 to rotate at a certain speed, at which the material rotates in the drum 2 without falling from the bottom, and a large centrifugal force can be generated to fully squeeze the solid / flocculated material. Preferably, the inner diameter of the drum 2 is 1.0-3.0 m, and the rotation speed is controlled to be 1200-3000 rpm; more preferably, the rotation speed is controlled to be 1200-1500 rpm.

[0061] S3, the mixed material prepared in step S1 is sucked by a delivery pump and pumped into the rotating drum 2 through the feed pipe 111 on the cover body 11 for rotary centrifugal treatment. The amount of mixed material pumped in at one time is controlled to avoid overflow caused by excessive mixing, which affects the subsequent filtering effect.

[0062] S4, after the pumped mixed material is centrifuged in the drum 2 for a certain period of time, the drum 2 is controlled to reduce its speed or stop rotating, so that the centrifuged material falls out from the bottom discharge port 12. Usually, a centrifugation treatment of 10 to 15 minutes can make the flocs aggregate into blocks, which is easy to filter and separate later.

[0063] S5, after the material to be centrifuged is discharged, restart or increase the rotation speed of the drum 2, so that the drum 2 rotates again at the speed required for centrifugation, and then repeat the operations of steps S3 to S5 to carry out the next round of material pumping and centrifugation until the mixed material processing is completed.

[0064] That is, only a certain amount of mixed material is processed in one high-speed rotation. After the material processed by rotation is discharged, the next mixed material is pumped in and the rotation centrifugal treatment is continued. This rotation centrifugal treatment method is an intermittent continuous treatment, and there is no need to stop the machine to clean the drum 2.

[0065] S6, the material discharged after the rotary centrifugal treatment is filtered through the filter element 16 to achieve solid-liquid separation, the separated liquid is allowed to stand for stratification, and then the aqueous phase and the organic solvent are separated to obtain an organic solvent containing bilirubin. The organic solvent containing bilirubin can be subjected to distillation and other processes to obtain the desired bilirubin.

[0066] The filter element 16 can be placed on the material receiving container 6 and located below the discharge port 12 . The material after the rotary centrifugal treatment falls into the filter element 16 and the material receiving container 6 by gravity and is quickly filtered by the filter element 16 .

[0067] The bilirubin separation and extraction method utilizes rotary centrifugation to squeeze the flocs in the mixed material into compact blocks, and then simple filtration can be used to achieve solid-liquid separation. The operation is simple and fast, and there is no need to clean the drum, etc., and automated and continuous production and processing can be achieved with high production efficiency. The bilirubin extraction rate is high, and less bilirubin residues are present in the separated floc impurities.

Claims

1. A three-phase separation device, characterized in that: include: An outer shell (1), wherein the interior of the outer shell (1) is a working chamber, the upper opening is provided with a cover (11) that can be opened and sealed, and the bottom opening is provided as a discharge port (12); A rotating drum (2) is coaxially rotatably disposed in the working chamber, and has an outer diameter smaller than an inner diameter of the working chamber; A driving member (3) is drivingly connected to the rotating drum (2) to drive the rotating drum (2) to rotate in the working chamber; in, The bottom of the rotating drum (2) is open; A feed pipe (111) is provided on the cover body (11), and the feed pipe (111) can extend into the rotating drum (2).

2. The three-phase separation device according to claim 1, characterized in that: It also comprises a drum head (4), which is coaxially arranged in the working chamber and fixedly connected to the drum tube (2) via a first bracket (41), and the drum head (4) is connected to the driving member (3).

3. The three-phase separation device according to claim 2, characterized in that: The driving member (3) comprises a first driving motor (31), a driving belt (32) and a main shaft; The upper portion of the main shaft is fixedly connected to the drum head (4), the middle portion is rotatably connected to the second bracket (34) via a bearing member (33), and the lower portion is transmission-connected to the output end of the first drive motor (31) via the transmission belt (32); The second bracket (34) is fixedly connected to the outer shell (1).

4. The three-phase separation device according to claim 2, characterized in that: The driving member (3) comprises a second driving motor, which is coaxially arranged at the lower part of the rotating drum head (4) and is connected and fixed to the outer shell (1) via a second bracket (34); the output end of the second driving motor is connected to the rotating drum head (4).

5. The three-phase separation device according to claim 1, characterized in that: When the cover body (11) is closed, the outlet of the feed pipe (111) is arranged toward the rotation direction of the inner wall of the rotating drum (2).

6. The three-phase separation device according to claim 5, characterized in that: The bottom of the side wall of the rotating drum (2) is provided with a circle of closing portion (21) extending inwardly; the projection position of the outlet of the feed pipe (111) is located within the projection area of ​​the closing portion (21).

7. The three-phase separation device according to claim 1 or 6, characterized in that: The side wall of the rotating drum (2) has no filter holes; Alternatively, the side wall of the rotary drum (2) has filter holes and is provided with an annular non-porous membrane bag adapted to the inner wall of the rotary drum (2); the non-porous membrane bag has openings at the top and bottom and can be detachably installed in the rotary drum (2).

8. The three-phase separation device according to claim 1, characterized in that: It also comprises a first mounting frame (5) and a second mounting frame (51), wherein the outer shell (1) is fixedly mounted on the first mounting frame (5), and the first mounting frame (5) is suspended and mounted on the second mounting frame (51) via a buffer member (52).

9. The three-phase separation device according to claim 8, characterized in that: Include one or more of the following characteristics: It also includes a material receiving container (6) and a filter element (61), wherein the filter element (61) is detachably mounted at the opening of the material receiving container (6) and can be placed under the second mounting frame (51) to correspond to the material outlet (12); It also includes a delivery pump, which is connected to the feed pipe (111) via a hose; A discharge barrel (121) is installed at the discharge port (12); A flushing port (13) is provided on the side wall of the outer shell (1), and the flushing port (13) is connected to a water pipe.

10. A method for separating and extracting bilirubin, characterized in that: The method is carried out using the three-phase separation device according to any one of claims 1 to 9, comprising the following steps: S1, adding water and an organic solvent to the bilirubin raw material solution, mixing them evenly, to obtain a mixed material; S2, closing the cover body (11) of the outer shell (1), starting the driving member (3) to drive the rotating drum (2) to rotate at a certain speed; S3, sucking a certain volume of the mixed material by a delivery pump, pumping it into the rotating drum (2) through a feed pipe (111), and rotating and centrifuging it; S4, after a certain period of rotary centrifugal treatment, the rotation speed of the rotary drum (2) is controlled to be reduced or stopped, so that the material after the rotary centrifugal treatment is discharged from the discharge port (12) at the bottom; S5, starting or increasing the rotation speed of the rotary drum (2) so that the rotary drum (2) rotates at a certain speed, and repeating steps S3 to S5 until the mixed material is processed; S6, the discharged material is separated into solid and liquid through a filter element (61), the separated liquid is allowed to stand for stratification, and the aqueous phase and the organic solvent are separated to obtain an organic solvent containing bilirubin.

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