Space-time selective optical excitation system based on laser scanning

Abstract

The invention provides a space-time selective optical excitation system based on laser scanning, which comprises an optical system, a real-time planning control system, an objective lens and an objective table, and is characterized in that the objective lens faces the objective table and is used for imaging a sample; the optical system comprises a laser beam expanding module, a galvanometer scanning module, an imaging monitoring module and a lighting module; the laser beam expanding module is used for emitting laser beams and collimating and expanding the laser beams; the galvanometer scanning module receives and manipulates the laser beam from the laser beam expanding module, so that the laser beam scans a sample positioned on an objective table in a two-dimensional plane in a specified mode; the imaging monitoring module is used for monitoring a laser scanning state; the illumination module is used for providing illumination for the objective lens; and the real-time planning control system is used for integrating and controlling the modules. According to the system, effective space-time selective light control can be carried out on the newly researched and developed light-driven continuous directional movement organic micro-nano crystal material, and the control convenience and practicability are improved.

Description

Technical Field

[0001] The invention relates to the technical field of light manipulation and optical microscopic imaging, and in particular to a spatiotemporal selective light excitation system based on laser scanning. Background Art

[0002] The spatiotemporal selective photoexcitation system is a technology that uses light to remotely control and manipulate the physical and chemical properties of target substances. It is widely used in scientific research and industrial production. By adjusting the wavelength, intensity, duration and spatial distribution of light, selective excitation of target substances at specific time and space points can be achieved. The spatiotemporal selective photoexcitation system can combine laser technology, optical engineering technology and software control technology to design and build a system according to actual needs to achieve selective programmed photoexcitation of target substances at the micro and nano scales.

[0003] In previous studies, we discovered a type of organic micro-nano crystals that have continuous directional motion driven by scanning light. Driven by scanning lasers, this type of crystal can produce a movement speed of micrometers per second, a movement accuracy of nanometers, and a continuous movement length of millimeters. However, commercial scanning laser systems, such as laser confocal systems or stimulated emission depletion super-resolution microscopy systems, have fixed laser light sources, scanning, and imaging modules, which limit the type of laser light source, the control of laser scanning, and the speed and method of real-time imaging. These limitations make it impossible to use commercial laser scanning systems to effectively perform spatiotemporal selective optical excitation and optical manipulation on the newly developed light-driven continuous directional motion organic micro-nano crystal materials. Therefore, it is necessary to develop a spatiotemporal selective optical excitation system based on laser scanning based on the motion characteristics of organic micro-nano crystals. Summary of the invention

[0004] The present invention aims to solve at least one of the technical problems in the related art to a certain extent. To this end, the present invention provides a spatiotemporal selective optical excitation system based on laser scanning, which includes the following requirements: 1. A stable laser light source with multiple switchable wavelengths and precisely adjustable light intensity; 2. A scanning laser excitation mode and multi-dimensional scanning parameter adjustment capabilities, such as scanning position, scanning speed, scanning direction, etc.; 3. A fast real-time imaging speed; 4. A planning control system with real-time programmed laser path planning scanning. The present invention develops a spatiotemporal selective optical excitation system based on laser scanning, combined with newly developed light-driven continuous directional motion organic micro-nano crystal materials, to achieve light-driven continuous motion along a planned path and precise spatial positioning of micro-nano crystal materials on various substrate surfaces in a non-solution environment (drying) with nanometer-level precision.

[0005] The spatiotemporal selective optical excitation system based on laser scanning of the present invention comprises an optical system, a real-time planning control system, an objective lens and an object stage, wherein the objective lens faces the object stage and is used to image the sample; the optical system comprises: a laser beam expansion module, a galvanometer scanning module, an imaging monitoring module and an illumination module; the laser beam expansion module is used to emit a laser beam and collimate and expand the laser beam; the galvanometer scanning module receives and manipulates the laser beam coming out of the laser beam expansion module so that the laser beam scans the sample located on the object stage in a specified manner within a two-dimensional plane; the imaging monitoring module is used to monitor the laser scanning state and perform real-time monitoring of the sample on the object stage; the illumination module is used to provide illumination for the objective lens; the real-time planning control system is used to integrate and control the laser beam expansion module, the galvanometer scanning module, the imaging monitoring module and the illumination module.

[0006] The spatiotemporal selective optical excitation system based on laser scanning of the present invention can effectively perform spatiotemporal selective optical manipulation on the newly developed light-driven continuous directional motion organic micro-nano crystal materials, thereby improving the convenience and practicality of the manipulation.

[0007] Optionally, the stage is a three-dimensional stage, used for placing samples, and capable of three-dimensional movement with micron-level precision;

[0008] The laser beam expansion module includes a multi-wavelength fiber-coupled laser, a laser beam expander, a first reflector and a second reflector; the multi-wavelength fiber-coupled laser is a laser light source that can provide multiple wavelengths including ultraviolet, visible light to near-infrared; the laser beam expander is connected to the multi-wavelength fiber-coupled laser and is used to expand and collimate the laser of the multi-wavelength fiber-coupled laser; the first reflector and the second reflector adjust the laser after collimation and expansion by the laser beam expander so that the collimated and expanded laser is incident on the center of the two-dimensional scanning galvanometer of the galvanometer scanning module to reduce the deviation of the spot position during the scanning process.

[0009] Optionally, the galvanometer scanning module includes a two-dimensional scanning galvanometer, a third reflecting mirror, a double-cemented achromatic lens and a fourth reflecting mirror;

[0010] The two-dimensional scanning galvanometer drives the adjustment of the galvanometer angle by providing a predetermined voltage value to the two scanning galvanometers of the X-axis and the Y-axis, thereby realizing scanning of the laser emitted by the laser beam expansion module in a two-dimensional plane; the third reflector and the fourth reflector are used to adjust the laser passing through the two-dimensional scanning galvanometer so that it can be incident on the center of the objective lens; the double-cemented achromatic lens is located between the optical paths of the third reflector and the fourth reflector, and is used to eliminate the dispersion phenomenon caused by lasers of different wavelengths.

[0011] Optionally, the two-dimensional scanning galvanometer is located at the first focal plane of the double-cemented achromatic lens, so that lasers of various wavelengths are at the same central position, and the central laser of the two-dimensional scanning galvanometer at the first focal plane is focused at the second focal plane.

[0012] Optionally, the spatiotemporal selective optical excitation system based on laser scanning also includes a second tube lens and a first dichroic mirror, wherein the first focal plane of the second tube lens is located at the second focal plane of the double-cemented achromatic lens and is combined with the objective lens so that the laser center point of the two-dimensional scanning galvanometer is conjugate with the focus of the objective lens imaging; the first dichroic mirror reflects the laser of the galvanometer scanning module to the center of the objective lens, and transmits the laser and sample image on the stage to the imaging monitoring module.

[0013] Optionally, the imaging monitoring module comprises: a sCMOS camera, a pull-out filter element and a first sleeve lens;

[0014] The sCMOS camera is used to monitor the sample condition and laser spot state of the stage, and compare the actual light scanning path with the planned set path in real time; the pull-out filter piece is arranged between the sCMOS camera and the first sleeve lens, and the pull-out filter piece can switch the filter by pulling; by switching the filter, the laser is blocked from imaging on the sCMOS camera, so that the visual effect is switched between three visual effects: only presenting the target sample state, only presenting the laser scanning state, and presenting the target sample and laser scanning state at the same time; the first sleeve lens is used to image the sample and laser on the stage on the sCMOS camera, so that the central laser of the two-dimensional scanning galvanometer is conjugate with the target surface of the sCMOS camera.

[0015] Optionally, the lighting module includes: a multi-wavelength LED fiber optic light source and an LED fiber optic beam expander; the multi-wavelength LED fiber optic light source is used to provide light source illumination with multiple wavelengths; the LED fiber optic beam expander is connected to the multi-wavelength LED fiber optic light source, and is used to convert the LED light source in the optical fiber into spatial light, and perform collimation and beam expansion; the LED fiber optic beam expander collimates and expands the light source, passes it through a plano-convex lens, and is reflected to the objective lens through a second dichroic mirror.

[0016] Optionally, the second dichroic mirror reflects the light source of the illumination module onto the stage, and transmits the illumination reflected light from the galvanometer scanning module and the laser on the stage and the sample.

[0017] Optionally, the laser wavelength emitted by the multi-wavelength fiber-coupled laser is in the range of 400-700nm; the laser wavelength emitted by the multi-wavelength LED fiber light source is in the range of 300-700nm; and the real-time planning and control system is a computer.

[0018] Optionally, the method for the real-time planning and control system to integrate and control the laser beam expansion module, the galvanometer scanning module, the imaging monitoring module and the lighting module includes:

[0019] Sending a wavelength selection instruction and a laser start instruction to the multi-wavelength fiber-coupled laser in the laser beam expansion module, so that the multi-wavelength fiber-coupled laser emits a laser beam to the two-dimensional scanning galvanometer; the two-dimensional scanning galvanometer controls the laser beam to scan, and the laser is transmitted to the stage through the first dichroic mirror and the second dichroic mirror to achieve spatiotemporal selective excitation of the sample on the stage, and then is emitted into the imaging monitoring module through the objective lens;

[0020] Sending voltage instructions to the X-axis and Y-axis scanning galvanometers of the two-dimensional scanning galvanometer in the galvanometer scanning module, so that the laser passes through the first sleeve lens and the objective lens to manipulate the sample on the stage according to a predetermined path, or manipulates the sample on the stage in real time, so as to be emitted into the imaging monitoring module through the objective lens;

[0021] Sending a wavelength selection instruction and an illumination start instruction to the illumination module so that the illumination module emits an illumination beam to the stage to image the sample on the stage, and transmits the illumination beam to the imaging monitoring module through the objective lens;

[0022] The collected image information and laser position are obtained from the imaging monitoring module, path planning or real-time manipulation is performed on the image information to obtain a path planning result or a real-time manipulation result, and the sample is manipulated according to the path planning or real-time manipulation.

[0023] Additional aspects and advantages of the present invention will be set forth in part in the description which follows and, in part, will be obvious from the description which follows, or may be learned by practice of the invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate embodiments consistent with the invention and, together with the description, serve to explain the principles of the invention.

[0025] Figure 1 Schematic diagram of a spatiotemporal selective optical excitation system based on laser scanning according to an embodiment of the present invention;

[0026] Figure 2 A schematic diagram of the optical path of a spatiotemporal selective optical excitation system based on laser scanning according to an embodiment of the present invention;

[0027] Figure 3 It is a simulated entity structure diagram of a laser beam expansion module and a galvanometer scanning module inside the optical system of a spatiotemporal selective optical excitation system based on laser scanning according to an embodiment of the present invention;

[0028] Figure 4 A schematic diagram of a laser scanning route of a spatiotemporal selective optical excitation system based on laser scanning and an optically driven directional movement route of an organic crystal material in an embodiment of the present invention;

[0029] Figure 5 This is an optical imaging diagram of the light-driven directional movement of an organic crystal material optically controlled by a spatiotemporal selective optical excitation system based on laser scanning according to an embodiment of the present invention;

[0030] Figure 6 A schematic diagram of a laser scanning route of a spatiotemporal selective optical excitation system based on laser scanning and a light-driven turning movement route of an organic crystal material in an embodiment of the present invention;

[0031] Figure 7 This is an optical imaging diagram of the movement of an organic crystal material along a target path optically manipulated by a spatiotemporal selective optical excitation system based on laser scanning according to an embodiment of the present invention.

[0032] Reference numerals:

[0033] 1-laser beam expansion module, 11-multi-wavelength fiber-coupled laser, 12-laser beam expander, 13-first reflector, 14-second reflector; 2-galvanometer scanning module, 21-two-dimensional scanning galvanometer, 22-third reflector, 23-double-glued achromatic lens, 24-fourth reflector; 3-imaging monitoring module, 31-sCMOS camera, 32-pull-out filter, 33-first sleeve lens; 4-illumination module, 41-multi-wavelength LED fiber light source, 42-LED fiber beam expander; 5-second sleeve lens, 6-first dichroic mirror, 7-second dichroic mirror, 8-objective lens, 9-stage, 10-plano-convex lens. DETAILED DESCRIPTION

[0034] The technical scheme of the present invention will be further described in detail below in conjunction with specific embodiments. It should be understood that the following embodiments are only exemplary descriptions and explanations of the present invention, and should not be construed as limiting the scope of protection of the present invention. All technologies implemented based on the above content of the present invention are included in the scope that the present invention is intended to protect.

[0035] This embodiment provides a spatiotemporal selective optical excitation system based on laser scanning, which mainly includes an optical system and a real-time planning control system. The optical system includes a laser beam expansion module 1, a galvanometer scanning module 2, an illumination module 4, and an imaging monitoring module 3. The real-time planning control system is used to integrate and control the four modules included in the above optical system. The spatiotemporal selective optical excitation system based on laser scanning can effectively perform spatiotemporal selective optical manipulation of the newly developed light-driven continuous directional motion organic micro-nano crystal materials, thereby improving the convenience and practicality of manipulation.

[0036] Figure 1 is a schematic diagram of a spatiotemporal selective optical excitation system based on laser scanning according to an embodiment of the present invention, Figure 2 FIG. 1 is a schematic diagram of an optical path of a spatiotemporal selective optical excitation system based on laser scanning according to an embodiment of the present invention; Figure 1 and Figure 2 The optical system of the spatiotemporal selective optical excitation system based on laser scanning is described in detail below. The optical system mainly includes a laser beam expansion module 1, a galvanometer scanning module 2, an illumination module 4 and an imaging monitoring module 3.

[0037] In this embodiment, combined with Figure 3 The laser beam expansion module 1 includes: a multi-wavelength fiber-coupled laser 11, a laser beam expander 12, a first reflector 13 and a second reflector 14. Among them, the multi-wavelength fiber-coupled laser 11 can select a laser wavelength suitable for the material to emit. More specifically, in this embodiment, the laser wavelength can be 405nm, 488nm, 532nm and 638nm, so as to correspond to the stimulation wavelength of the manipulated material. Furthermore, the multi-wavelength fiber-coupled laser 11 can be connected to a computer, and the corresponding laser wavelength and power can be selected, and the switch and size of the laser can be controlled in a certain time sequence; the laser beam expander 12 is used to collimate and expand the laser from the multi-wavelength fiber-coupled laser 11 to obtain a collimated light beam; the first reflector 13 and the second reflector 14 are used to adjust the position of the collimated light beam so that the center of the laser is located at the center of the two-dimensional scanning galvanometer 21 of the galvanometer scanning module 2.

[0038] The galvanometer scanning module 2 is used to manipulate the laser so that it scans the sample in a specified manner in a two-dimensional plane. Figure 3, the galvanometer scanning module 2 includes: a two-dimensional scanning galvanometer 21, a third reflector 22, a double-glued achromatic lens 23 and a fourth reflector 24. Among them, the two-dimensional scanning galvanometer 21 can manipulate the laser in real time and plan the path according to the desired path. Furthermore, by giving a certain voltage value to the X-axis galvanometer and the Y-axis galvanometer of the two-dimensional scanning galvanometer 21, the deflection angle of the galvanometer is controlled, and then the deflection of the X-axis and Y-axis galvanometers is combined, the scanning of the laser in the two-dimensional plane can be realized. More specifically, in this embodiment, the two-dimensional scanning galvanometer 21 is connected to the computer and can manipulate the sample surface photographed by the sCMOS camera 31. In terms of software integration, the scanning galvanometer can be controlled in a visualized and planable form on the X and Y axis coordinates to realize the manipulation of the two-dimensional plane; wherein, visualization refers to the real-time control of the laser spot on the screen by manually using a mouse to collect pictures, and planability refers to the method of using a command set and a mouse to edit together to formulate a series of paths, such as a circle, a rectangle, a straight line and a custom route, and the moving speed automatically controls the laser to move according to the planned path. The third reflecting mirror 22 and the fourth reflecting mirror 24 are used to adjust the laser light emitted from the two-dimensional scanning galvanometer 21 so that the laser light can be incident on the center of the objective lens 8 .

[0039] The double-cemented achromatic lens 23 is used to eliminate the chromatic aberration caused by different laser wavelengths, so that all laser points of the laser with a wavelength within a certain range are at the same diameter and center. On the other hand, a two-dimensional scanning galvanometer 21 is placed at the first focal plane of the double-cemented achromatic lens 23, so that the laser center point of the two-dimensional scanning galvanometer 21 converges at the second focal plane.

[0040] The laser reflected by the fourth reflector 24 passes through the first sleeve lens 5 and is reflected by the first dichroic mirror 6 to the objective lens 8, and the objective lens 8 focuses the laser reflected by the first dichroic mirror 6 on the three-dimensional stage 9. The first focal plane of the first sleeve lens 5 is located at the second focal plane of the double-cemented achromatic lens 23, so that the laser focused on this position is a collimated light beam after passing through the first sleeve lens 5. The first dichroic mirror 6, on the one hand, can reflect the laser passing through the first sleeve lens 5, so that the collimated light beam is focused on the sample surface on the three-dimensional stage 9 after passing through the objective lens 8. On the other hand, the first dichroic mirror 6 transmits the laser reflected back from the sample surface and images it on the target surface of the sCMOS camera 31 of the imaging monitoring module 3.

[0041] In some embodiments, in the galvanometer scanning module 2, the center point of the two-dimensional scanning galvanometer 21 is located at the first focal plane of the double-cemented achromatic lens 23, and the center point is conjugate with the second focal plane of the double-cemented achromatic lens 23. Since the second focal plane of the double-cemented achromatic lens 23 coincides with the first focal plane of the first sleeve lens 5, and there is a collimated light beam between the first sleeve lens 5 and the objective lens 8, the first focal plane of the first sleeve lens 5 is conjugate with the focus of the objective lens, that is, the center point of the two-dimensional scanning galvanometer is conjugate with the focal point of the objective lens.

[0042] The three-dimensional stage 9 is used to place the sample and move it in three dimensions. Furthermore, the three-dimensional stage 9 is connected to a computer and integrated into the software, and the three-dimensional movement of the three-dimensional stage can be controlled according to a desired timing.

[0043] The lighting module 4 is used for sample illumination. In the present embodiment, the lighting module 4 includes: a multi-wavelength LED fiber optic light source 41 and an LED fiber optic collimator 42. Among them, the multi-wavelength LED fiber optic light source 41 can select an LED wavelength suitable for the material to emit. More specifically, in the present embodiment, the LED wavelength can be 385nm, 460nm, 550nm and 625nm. Furthermore, the multi-wavelength LED fiber optic light source 41 can be connected to a computer, and the corresponding LED wavelength and power can be selected, and the switch and size of the light source can be controlled in a certain time sequence; the LED fiber optic collimator 42 is used to convert the fiber optic light source into a spatial light source and collimate it. After the LED fiber optic collimator 42 converts the fiber optic light source into a spatial light source and collimates it, it is reflected by the plano-convex lens 10 and the second dichroic mirror 7 and then passes through the objective lens 8 to illuminate the sample.

[0044] That is to say, the plano-convex lens 10 is used to further collimate the LED light source of the LED fiber beam expander 42 to obtain a higher power illumination source and a more suitable illumination field. The second dichroic mirror 7 is used to reflect the light source from the illumination module 4, and illuminate the sample after passing through the objective lens 8. On the other hand, the second dichroic mirror 7 is used to transmit the reflected light from the sample, and after passing through the first dichroic mirror 6, it is collected by the imaging monitoring module 3 to obtain sample information. The objective lens focuses the LED illumination light, and cooperates with the plano-convex lens 10 to obtain a uniform illumination light source that fills the camera imaging target surface.

[0045] The imaging monitoring module 3 is used to monitor the sample and the laser in real time. In this embodiment, the imaging monitoring module 3 mainly includes: an sCMOS camera 31, a pull-out filter element 32 and a second sleeve lens 33. The sCMOS camera 31 is used to monitor the sample and the laser in real time. Furthermore, the sCMOS camera 31 has better quantum efficiency, can better collect weak signals, and has better imaging quality. More specifically, the sCMOS camera 31 is connected to a computer and integrated in the software, and can directly perform path planning and real-time manipulation of laser scanning, switching and timing changes of the illumination light source, and switching and timing changes of the scanning laser on the camera acquisition screen.

[0046] The pull-out filter 32 is a component that installs a filter by pulling it out, and the filter can be pulled out to switch between filters. By switching the filter, the laser imaging on the sCMOS camera 31 can be blocked, so that the three screens of monitoring only the sample state, only detecting the laser scanning state, and monitoring the sample state and the laser scanning state at the same time can be switched. Furthermore, since the laser light intensity is generally stronger than the illumination light source, the laser will block the observation of the sample. By switching the filter of the pull-out filter 32, the laser can be shielded and only the information of the sample can be collected, but at the same time it does not affect the operation of the laser scanning.

[0047] The second sleeve lens 33 is used to collect the laser and sample reflected light on the three-dimensional stage 9, and monitor the imaging through the sCMOS camera 31; the objective lens 8 is used in conjunction with the second sleeve lens 33 to collect the laser and sample reflected light on the three-dimensional stage 9, so that the camera target surface is conjugate with the sample surface, and is also conjugate with the laser scanning point.

[0048] In some embodiments, a wavelength selection instruction and a laser start instruction are sent to the multi-wavelength fiber-coupled laser 11 in the laser beam expansion module 1 so that the multi-wavelength fiber-coupled laser 11 emits a laser beam to the two-dimensional scanning galvanometer 21, so that the two-dimensional scanning galvanometer 21 scans the laser beam, and the laser beam is transmitted to the stage 9 through the first dichroic mirror 6 and the second dichroic mirror 7 to manipulate the object to be observed on the stage, and then enters the imaging monitoring module 3 through the objective lens 8. A voltage instruction is sent to the X-axis and Y-axis scanning galvanometers of the two-dimensional scanning galvanometer in the galvanometer scanning module 2 so that the laser beam passes through the first sleeve lens 33 and the objective lens 8 and manipulates the sample on the stage 9 according to a certain path, or manipulates the sample on the stage 9 in real time, and then enters the imaging monitoring module 3 through the objective lens 8. Control voltages corresponding to the X-axis, Y-axis and Z-axis are input to the stage 9 to control the movement of the three-dimensional stage as required.

[0049] The wavelength selection instruction and the lighting start instruction are sent to the lighting module 4 so that the lighting module 4 emits a lighting beam to the stage 9 to image the object to be observed on the stage 9, and transmits it to the imaging monitoring module 3 through the objective lens 8; the imaging monitoring module 3 collects image information and laser position, and performs path planning or real-time manipulation on the image information to obtain the path planning result or the real-time manipulation result, and manipulates the sample according to the path planning or real-time manipulation. By connecting the above modules (laser beam expansion module 1, galvanometer scanning module 2, imaging monitoring module 3 and lighting module 4) with a computer, the above modules can be controlled in real time, and multiple wavelengths can be switched in real time, and the light intensity can be precisely adjusted; the scanning parameter adjustment capability of multiple dimensions such as scanning position, scanning speed, scanning direction, etc. can be performed in real time; the sample position information collected by the imaging monitoring module is used to perform real-time synchronous sample manipulation or real-time update of the path planning of the manipulated sample. The required scanning path can also be preset and planned in advance.

[0050] This embodiment further provides the following two examples.

[0051] Example 1: A suspension of organic crystal material capable of achieving light-driven directional movement is first dripped onto the surface of a quartz plate and the solvent is blown dry with nitrogen. A quartz plate with organic crystals randomly distributed on the surface is placed on the stage 9 of the spatiotemporal selective light excitation system. First, switch to a 40x objective lens and observe in the bright field mode of the microscope. By adjusting the Z-axis position, the laser spot can be focused on the focal plane of the organic crystal material, and at the same time, the imaging focal plane position is adjusted to achieve clear focus imaging. Figure 4 As shown, the laser scanning path is set to coincide with the long axis center of the organic crystal material that is directional moved by light, and a loop execution path is set. Other specific parameters: the wavelength of the execution laser is set to 638nm, and the scanning speed of the execution laser is set to 10,000 points / second. Under the control of the spatiotemporal selective light excitation system, the spatial position change of the organic crystal material is monitored in real time through the imaging monitoring module 3. Figure 5 As shown, the organic crystal material driven by light to move in a directional manner moves in a straight line along the long axis of the crystal in the opposite direction to the laser scanning direction.

[0052] Example 2: A quartz plate with organic crystals randomly distributed on its surface is placed on the stage 9 of the spatiotemporal selective light excitation system. First, switch to a 40x objective lens and observe in the bright field mode of the microscope. By adjusting the Z-axis position, the laser spot can be focused on the focal plane of the organic crystal material, and at the same time, the imaging focal plane position is adjusted to achieve clear focus imaging. Figure 6As shown, the angle between the starting laser scanning path and the long axis of the organic crystal material is set to 4 degrees and a loop execution path is set. Other specific parameters: the wavelength of the execution laser is set to 638nm, and the scanning speed of the execution laser is set to 10,000 points / second. Under the control of the spatiotemporal selective light excitation system, the spatial position changes of the organic crystal material are monitored in real time through the imaging monitoring module 3. During the movement of the organic crystal material, the turning direction and radius of the crystal movement can be precisely controlled by changing the angle between the scanning laser trajectory and the long axis of the crystal. Figure 7 As shown, the organic crystal material with light-driven directional movement continues to move along the target path under laser control.

[0053] In the description of the present invention, it is to be understood that the terms “center”, “longitudinal”, “lateral”, “length”, “width”, “thickness”, “up”, “down”, “front”, “back”, “left”, “right”, “vertical”, “horizontal”, “top”, “bottom”, “inside”, “outside”, “clockwise”, “counterclockwise”, “axial”, “radial”, “circumferential”, etc., indicating orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as limiting the present invention.

[0054] In addition, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include at least one of the features. In the description of the present invention, the meaning of "plurality" is at least two, such as two, three, etc., unless otherwise clearly and specifically defined.

[0055] In the present invention, unless otherwise clearly specified and limited, the terms "installed", "connected", "connected", "fixed" and the like should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral one; it can be a mechanical connection, an electrical connection, or communication with each other; it can be a direct connection, or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements, unless otherwise clearly defined. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0056] In the present invention, unless otherwise clearly specified and limited, a first feature being "above" or "below" a second feature may mean that the first and second features are in direct contact, or the first and second features are in indirect contact through an intermediate medium. Moreover, a first feature being "above", "above" or "above" a second feature may mean that the first feature is directly above or obliquely above the second feature, or simply means that the first feature is higher in level than the second feature. A first feature being "below", "below" or "below" a second feature may mean that the first feature is directly below or obliquely below the second feature, or simply means that the first feature is lower in level than the second feature.

[0057] In the present invention, the terms "one embodiment", "some embodiments", "examples", "specific examples", or "some examples" etc. mean that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the described specific features, structures, materials or characteristics may be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art may combine and combine the different embodiments or examples described in this specification and the features of the different embodiments or examples, without contradiction.

[0058] Although the embodiments of the present invention have been shown and described above, it is to be understood that the above embodiments are exemplary and are not to be construed as limitations on the present invention. A person skilled in the art may change, modify, replace and modify the above embodiments within the scope of the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention shall be included in the scope of protection of the present invention.

Claims

1. A spatiotemporal selective optical excitation system based on laser scanning, characterized in that: It comprises an optical system, a real-time planning control system, an objective lens (8) and an object stage (9), wherein the objective lens (8) faces the object stage (9), and the objective lens (8) is used for imaging a sample; The optical system comprises: a laser beam expansion module (1), a galvanometer scanning module (2), an imaging monitoring module (3) and an illumination module (4); the laser beam expansion module (1) is used to emit a laser beam and collimate and expand the laser beam; the galvanometer scanning module (2) receives and manipulates the laser beam emitted from the laser beam expansion module (1) so that the laser beam scans a sample located on the stage (9) in a specified manner within a two-dimensional plane; the imaging monitoring module (3) is used to monitor the laser scanning state and perform real-time monitoring of the sample on the stage (9); the illumination module (4) is used to provide illumination for the objective lens (8); The real-time planning control system is used to integrate and control the laser beam expansion module (1), the galvanometer scanning module (2), the imaging monitoring module (3) and the lighting module (4).

2. The spatiotemporal selective optical excitation system based on laser scanning according to claim 1, characterized in that: The stage (9) is a three-dimensional stage, used for placing samples, and capable of three-dimensional movement with micron-level precision; The laser beam expansion module (1) comprises a multi-wavelength fiber-coupled laser (11), a laser beam expander (12), a first reflector (13) and a second reflector (14); the multi-wavelength fiber-coupled laser (11) is a laser light source capable of providing a plurality of wavelengths ranging from ultraviolet, visible light to near infrared; the laser beam expander (12) is connected to the multi-wavelength fiber-coupled laser (11) and is used to expand and collimate the laser beam of the multi-wavelength fiber-coupled laser (11); the first reflector (13) and the second reflector (14) adjust the laser beam after collimation and expansion by the laser beam expander (12), so that the collimated and expanded laser beam is incident on the center of the two-dimensional scanning galvanometer (21) of the galvanometer scanning module (2), so as to reduce the deviation of the light spot position during the scanning process.

3. The spatiotemporal selective optical excitation system based on laser scanning according to claim 2, characterized in that: The galvanometer scanning module (2) comprises a two-dimensional scanning galvanometer (21), a third reflecting mirror (22), a double-cemented achromatic lens (23) and a fourth reflecting mirror (24); The two-dimensional scanning galvanometer (21) achieves scanning of the laser light emitted by the laser beam expansion module (1) in a two-dimensional plane by providing a predetermined voltage value to the two scanning galvanometer mirrors on the X-axis and the Y-axis to drive the adjustment of the galvanometer mirror angles; the third reflecting mirror (22) and the fourth reflecting mirror (24) are used to adjust the laser light passing through the two-dimensional scanning galvanometer mirror (21) so that it can be incident on the center of the objective lens (8); the double-cemented achromatic lens (23) is located between the optical paths of the third reflecting mirror (22) and the fourth reflecting mirror (24) and is used to eliminate the dispersion phenomenon caused by laser light of different wavelengths.

4. The spatiotemporal selective optical excitation system based on laser scanning according to claim 3, characterized in that: The two-dimensional scanning galvanometer (21) is located at the first focal plane of the double-cemented achromatic lens (23), so that lasers of various wavelengths are located at the same central position, and the central laser of the two-dimensional scanning galvanometer (21) at the first focal plane is focused at the second focal plane.

5. The spatiotemporal selective optical excitation system based on laser scanning according to claim 3, characterized in that: It also includes a second sleeve lens (5) and a first dichroic mirror (6), wherein the first focal plane of the second sleeve lens (5) is located at the second focal plane of the double-cemented achromatic lens (23), and is combined with the objective lens (8) so that the laser center point of the two-dimensional scanning galvanometer (21) is conjugate with the focus of the objective lens imaging; the first dichroic mirror (6) reflects the laser of the galvanometer scanning module (2) to the center of the objective lens (8), and transmits the laser and the sample image on the stage (9) to the imaging monitoring module (3).

6. The spatiotemporal selective optical excitation system based on laser scanning according to claim 3, characterized in that: The imaging monitoring module (3) comprises: an sCMOS camera (31), a pull-out filter element (32) and a first sleeve lens (33); The sCMOS camera (31) is used to monitor the sample status and laser spot status of the stage (9), and to compare the actual light scanning path with the planned set path in real time; the pull-out filter element (32) is arranged between the sCMOS camera (31) and the first sleeve lens (33), and the pull-out filter element (32) can switch the filter by pulling; by switching the filter, the laser is blocked from imaging on the sCMOS camera (31), so that the visual effect is switched between three visual effects: only presenting the target sample state, only presenting the laser scanning state, and presenting the target sample and laser scanning state at the same time; the first sleeve lens (33) is used to image the sample and laser on the stage (9) on the sCMOS camera (31), so that the central laser of the two-dimensional scanning galvanometer (21) is conjugated with the target surface of the sCMOS camera (31).

7. The spatiotemporal selective optical excitation system based on laser scanning according to claim 6, characterized in that: The lighting module (4) comprises: a multi-wavelength LED fiber optic light source (41) and an LED fiber optic beam expander (42); the multi-wavelength LED fiber optic light source (41) is used to provide light source illumination with multiple wavelengths; the LED fiber optic beam expander (42) is connected to the multi-wavelength LED fiber optic light source (41) and is used to convert the LED light source in the optical fiber into spatial light and perform collimation and beam expansion; the LED fiber optic beam expander (42) collimates and expands the light source, passes through a plano-convex lens (10), and is reflected to the objective lens (8) via a second dichroic mirror (7).

8. The spatiotemporal selective optical excitation system based on laser scanning according to claim 7, characterized in that: The second dichroic mirror (7) reflects the light source of the illumination module (4) onto the stage (9), and transmits the laser light from the galvanometer scanning module (2) and the stage (9) as well as the illumination reflected light of the sample.

9. The spatiotemporal selective optical excitation system based on laser scanning according to claim 7, characterized in that: The wavelength range of the laser emitted by the multi-wavelength fiber-coupled laser (11) is 400-700 nm; the wavelength range of the laser emitted by the multi-wavelength LED fiber-optic light source 41 is 300-650 nm; and the real-time planning control system is a computer.

10. The spatiotemporal selective optical excitation system based on laser scanning according to any one of claims 7 to 9, characterized in that: The method for the real-time planning control system to integrate and control the laser beam expansion module (1), the galvanometer scanning module (2), the imaging monitoring module (3) and the lighting module (4) comprises: A wavelength selection instruction and a laser start instruction are sent to the multi-wavelength fiber-coupled laser (11) in the laser beam expansion module (1), so that the multi-wavelength fiber-coupled laser (11) emits a laser beam to the two-dimensional scanning galvanometer (21); the two-dimensional scanning galvanometer (21) controls the laser beam to scan, and the laser is transmitted to the stage (9) via the first dichroic mirror (6) and the second dichroic mirror (7) to achieve spatiotemporal selective excitation of the sample on the stage (9), and then injected into the imaging monitoring module (3) via the objective lens (8); sending voltage instructions to the X-axis and Y-axis scanning galvanometers of the two-dimensional scanning galvanometer (21) in the galvanometer scanning module (2), so that the laser light, after passing through the first sleeve lens (5) and the objective lens (8), manipulates the sample on the stage (9) according to a predetermined path, or manipulates the sample on the stage (9) in real time, so as to be emitted into the imaging monitoring module (3) through the objective lens (8); sending a wavelength selection instruction and an illumination start instruction to the illumination module (4) so ​​that the illumination module (4) emits an illumination light beam to the object stage (9) to image the sample on the object stage (9), and transmits the illumination light beam to the imaging monitoring module (3) via the objective lens (8); The collected image information and laser position are obtained from the imaging monitoring module (3), path planning or real-time manipulation is performed on the image information to obtain a path planning result or a real-time manipulation result, and the sample is manipulated according to the path planning or real-time manipulation.

Images