Method for reducing saline-alkali water into modern seawater by using microorganisms to be suitable for culturing various seafood, special fish fertilizer for method and application of preparation method of special fish fertilizer

Abstract

The invention discloses a method for reducing saline-alkali water into modern seawater suitable for culturing various seafoods by using microorganisms, a special fish fertilizer for the method and application of a preparation method of the special fish fertilizer, and the method comprises the following specific steps: 1, pretreating the saline-alkali water: pumping saline-alkali with the salinity of 15-30% o into a culture pond, compressing, mixing and precipitating until the pH value is 7.3-7.5; 2, performing microbial culture: performing multi-stage enrichment on halobacterium violaceum and selecting dominant colonies; the method comprises the following steps: mixing underground saline-alkali water with the salinity of 10-20% o, saline-alkali water of a salt lake with the salinity of 180-200% o and high saline-alkali soil containing part of organic matters, cooking and sealing at the temperature of 200-210 DEG C, inoculating selected mixed halobacterium purple, pumping air, culturing with sunlight, and propagating for later use, wherein the pH value is 8.3-8.5; 3, reduction reaction: carrying out reduction reaction in a static water environment, when the water body is red brown or dark brown, inoculating a heterotrophic photosynthetic bacteria liquid and an autotrophic photosynthetic bacteria liquid with concentration, inoculating yeast powder, carrying out common reaction, then inoculating a special aquaculture fish fertilizer preparation, carrying out oxygenation reaction, and adjusting the pH value to 8.5-8.8; real seafood can be cultured in saline-alkali water in deserts and gobi deserts, zero pollution and zero emission can be achieved, the cost of development, implementation and popularization is low, and the cost of 10,000 cubic meters of water is only 675 yuan.

Description

Technical Field

[0001] The invention relates to a method for cultivating various seafoods by utilizing microorganisms, in particular to a method for reducing saline-alkali water into modern seawater suitable for cultivating various seafoods by utilizing microorganisms, and the application of the special fish fertilizer and the preparation method thereof. Background Art

[0002] At present, the components in modern seawater can be divided into five categories: 1. Main components (majority, constant elements): refers to the components with a concentration greater than 1 mg / kg in seawater. + , K + , Ca 2+ Mg 2+ and Sr 2+ Five, anion Cl - 、SO4 2- Br - 、HCO3 - 、CO3 2- 、F - Six, and H3B03 in molecular form, the sum of which accounts for 99.9% of the salt content of seawater. So they are called the main components. Since the content of these components in seawater is relatively large, the concentration ratio of each component is approximately constant, and biological activities and changes in total salinity have little effect on them, so they are called conservative elements. The Si content in seawater is sometimes greater than 1 mg / kg, but because its concentration is greatly affected by biological activities and its properties are unstable, it is a non-conservative element, so Si is not included when discussing the main components. 2. Gas components dissolved in seawater, such as oxygen, nitrogen and inert gases. 3. Nutrient elements (nutrients, biogenic elements): mainly elements related to the growth of marine plants, usually referring to N, P and Si. The content of these elements in seawater is often affected by plant activities. When their content is very low, it will limit the normal growth of plants, so these elements are of great significance to organisms. 4. Trace elements: those with very low content in seawater, but not nutrient elements. 5. Organic matter in seawater: such as amino acids, humus, chlorophyll, etc.

[0003] Although various salts are dissolved in seawater, the cause of seawater salinity is a complex issue, which is related to the origin of the earth, the formation and evolution of the ocean. It is generally believed that salt mainly comes from the weathering products of crustal rocks and soluble compounds ejected by submarine volcanoes. In addition, rivers around the world transport 5.5×1015g of dissolved salt to the ocean every year, which is also one of the sources of seawater salinity. Judging from its source, seawater seems to contain all the elements on the earth, but due to the limitations of analytical level, only more than 80 kinds have been measured. Some low-content components are difficult to measure, and there are not many samples that have been measured, so it is difficult to represent their average concentration. Many metals of interest are extremely low in seawater and can only be measured using sensitive testing instruments and techniques and avoiding contamination during sample collection and analysis.

[0004] Looking at my country's aquaculture industry, according to the traditional breeding model, the general method is to use chemical fertilizers to cultivate aquatic organisms, use chemical bottom modification or mineral water body conditioners to treat the environment, and use chemical fertilizers mixed with some common bacteria or probiotics for mixing and splashing. The specific method is: 20-30 catties of chemical fertilizers are added to 1-2 catties of common bacteria or probiotics per mu each time, generally 10-12 times a month, and chemical or mineral conditioners are applied 5-6 times; according to scientific analysis, the utilization rate of chemical fertilizers applied to aquaculture water bodies by this method is 10-20%, and the utilization rate of chemical minerals is 5-10%. The rest are deposited at the bottom of the pond and discharged into the inland river and the sea, directly wasting about 1.2 billion kg of chemical fertilizers, chemicals, and mineral preparations every year. my country's inland waters and offshore waters have been polluted and eutrophicated to varying degrees, so it is the unshirkable responsibility of each of us to protect the natural environment on which we depend for survival and to protect the sea that can create our brilliant career.

[0005] To sum up, in addition to protecting the earth's natural aquaculture water sources, it is very important to find other natural water sources suitable for aquaculture seafood.

[0006] my country has about 690 million mu of low-lying saline-alkali waters and inland saltwater areas accounting for about 55% of the country's lake area. These saline-alkali land (water) resources are spread across 17 provinces, municipalities, and autonomous regions, mainly distributed in the inland areas of Northeast China, North China, and Northwest China. The saline-alkali waters in these areas are mostly non-marine salt water with complex water chemistry. They are not drinkable for humans and animals, cannot be used to irrigate farmland, and cannot be directly used for aquaculture. Therefore, most of them are in a state of long-term abandonment. For example, Dalai Lake in Inner Mongolia, Qinghai Lake in Qinghai, and Buluntohai in Xinjiang are all typical saline-alkali waters.

[0007] The pH value of saline water is generally above 8. The pH value of saline water is affected by its salinity and chemical composition, and usually presents alkaline characteristics. The salinity of saline water is generally between 5‰ and 30‰, and this salinity range has certain restrictions on the growth of plants and animals. The formation of saline-alkali land is often related to the evaporation of groundwater and surface water. During this process, the salt concentration in the water gradually increases, causing the water body to become alkaline. The change in the pH value of saline water has an important impact on the organisms living in it. For example, for fish, the pH value range of water bodies suitable for survival is usually between 6.5 and 8.5. When the pH value of the water body exceeds this range, especially when it deviates seriously in the alkaline direction, the fish may become uncomfortable or even die. This is because the change in pH value affects the chemical balance in the water body, and then affects the toxicity of the chemicals in the water. For example, when the pH value increases, the ammonia nitrogen in the water exists in the form of non-ionized ammonia, which is highly toxic to fish because it has a fairly high fat solubility and can penetrate the cell membrane to poison the cells. Therefore, understanding and controlling the pH value of saline water bodies is crucial to protecting the health of aquatic organisms. In practical applications, measures may need to be taken to adjust the pH value of water bodies to ensure that it is within an appropriate range, thereby maintaining the stability of the ecosystem and the living conditions of organisms.

[0008] A friend went to Xinjiang for a trip, and a joke that "it's not fun to not be able to eat fresh seafood there" made the inventor fall into deep thinking. The inventor asked a friend to send some local saline water from Xinjiang to the inventor, thinking that it would be wonderful if the local saline water in Xinjiang could be used for real seafood farming through technical means. So the inventor asked his friend to find local salt lake water or underground saline water in Xinjiang, and then tested it. The results showed that the salinity of the salt lake water was 270‰ and the salinity of the underground saline water was 8‰. Salt lake water with a salinity of 270‰ is not suitable for the survival of marine life. Even if the underground saline water with a salinity of 8‰ is restored to seawater, it can only breed some low-end "fake seafood", that is, river fresh, such as white shrimp, lobster, California sea bass, etc., while high-end real seafood such as East Star Grouper, Tiger Grouper, Pearl Grouper, Yellow Flag Snapper, Sea Lobster, Abalone, etc., are difficult to survive in this environment. In the Xinjiang Uygur Autonomous Region, there are almost no fish living in the many salt lakes. The reason is that the substances contained in the lakes are mainly chemical components such as sodium chloride, sodium sulfate, and nitrates, which are impossible for marine life to survive.

[0009] Definition of seafood and river fish:

[0010] In the vast world of food, seafood, river fish and river fish have always been popular delicacies. However, not everyone knows their exact definitions. Seafood lives in seawater environments, river fish live in brackish water environments, and river fish grow in freshwater environments.

[0011] In recent years, a new trend of seafood farming has been set off in inland areas such as Xinjiang, Ningxia, and Qinghai. But for most people, the understanding of seafood may still remain at the traditional level. In the true sense, seafood must rely on seawater for its growth and breeding environment. Usually, fish, shrimps, and crabs that survive in waters with a salinity of 15‰ to 20‰ or more can be called seafood, such as pearl grouper, eastern star grouper, and other types of grouper, yellow snapper, mud fish, golden pomfret, golden drum fish, large yellow croaker, octopus, tuna, etc., as well as abalone, sea cucumber, swimming crab, green lobster, car shrimp, tiger prawn, etc. The brackish and freshwater fish, shrimps, and crabs that live at the confluence of rivers and seas with a salinity below 8‰ are called river fresh.

[0012] Fish, shrimps and crabs that grow in saline water with a salinity of less than 3‰ and freshwater with a salinity of less than 0‰ are all classified as river freshwater. It should be emphasized that in water with a salinity of 3‰ and freshwater environments, only a few species can be desalinated and adapted to be farmed in freshwater rivers and lakes. Such as white shrimp, California bass, hairy crabs, blue crabs, Macrobrachium rosenbergii, eels, salmon, etc. Since these species grow in freshwater environments, they should be judged as river freshwater by definition. Moreover, there are obvious differences in taste and quality between seafood, river freshwater and river freshwater.

[0013] Clearly and accurately defining seafood, river fish and stream fish will not only help us know the source and characteristics of the ingredients more accurately.

[0014] In order to develop a method and process for reducing Xinjiang's saline-alkali water into modern seawater suitable for breeding various seafood, the inventor traveled throughout the southern Xinjiang region for three years and found that various breeding units were trying to add fresh water to the saline-alkali water and then add various seawater elements.

[0015] The disadvantages of these methods of adding fresh water to saline water and then adding various seawater elements are:

[0016] 1) This method is very costly.

[0017] The steps of this method of adding seawater elements to fresh water are as follows: (preparing 10-12‰ seawater)

[0018] 1. Take in 12 cubic meters of fresh water;

[0019] 2. Add 62.5kg of coarse salt;

[0020] 3. Magnesium sulfate 35kg;

[0021] 4. Calcium chloride 4kg;

[0022] 5. Potassium chloride 2kg;

[0023] 6. Sodium octaborate tetrahydrate 1.21 kg;

[0024] 7. Potassium bromide 180g;

[0025] 8. Phosphoric acid 25g;

[0026] 9. On the first day of stocking, add 5kg of lactic acid, 2 scoops of algae, and 200g of quick supplement = 25 yuan. Add 200g of multivitamin 10 minutes before stocking = 25 yuan.

[0027] The cost is calculated as follows: (preparing 10-12‰ seawater)

[0028] 1. Take in 12 cubic meters of fresh water;

[0029] 2. Add 62.5kg of coarse salt × 2 yuan = 125 yuan;

[0030] 3. Magnesium sulfate 35kg × 1.6 yuan = 56 yuan;

[0031] 4. Calcium chloride 4kg × 2 yuan = 8 yuan;

[0032] 5. Potassium chloride 2kg × 3 yuan = 6 yuan;

[0033] 6. Sodium octaborate tetrahydrate 1.21kg × 10 yuan = 12.1 yuan;

[0034] 7. Potassium bromide 180g = 5 yuan;

[0035] 8. Phosphoric acid 25g = 5 yuan;

[0036] 9. On the first day of stocking, add 5kg of lactic acid × 10 yuan = 50 yuan, 2 scoops of algae, 200g of quick supplement = 25 yuan. Add 200g of multivitamin 10 minutes before stocking = 25 yuan. The above materials cost about 317.1 yuan.

[0037] 317.19÷12 cubic meters of water=26.425 / cubic meter of water.

[0038] For example, a 15-mu pond with a water depth of 1 meter contains about 10,000 cubic meters of 10-12‰ seawater x 26.425 yuan = 264,250 yuan.

[0039] The above formula can be adjusted to 1.0-1.2% seawater (10-12 parts per thousand seawater). The difference varies according to the substances contained in the fresh water used, but the difference is not big. If the seawater required for seafood farming is 20-22‰, 15 mu pond with a water depth of 1 meter and about 10,000 cubic meters of seawater will cost 528,500 yuan.

[0040] 2) This method cannot be used to farm seafood. It can only farm river fish, not real seafood.

[0041] 3) Due to the local policy in Xinjiang, it is not allowed to discharge water after aquaculture. The general terrain is desert and Gobi, and there are no rivers for discharge. The breeding pond cannot achieve zero pollution and zero emission without changing the water. Therefore, the effect of breeding river fresh is extremely poor, the survival rate is low, and it is even more impossible to breed real seafood.

[0042] 4) This method of breeding requires the fry to be domesticated before breeding. Summary of the invention

[0043] The purpose of the present invention is to provide a method for reducing saline-alkali water into seawater suitable for breeding various seafood by using microorganisms, and the application of its special fish fertilizer and preparation method. It is not only a pure natural and green biological preparation, but also an ideal substitute for antibiotics, and can effectively improve the output, quality, feed conversion rate and economic benefits of aquatic products.

[0044] In order to solve the above technical problems, the technical solution of the present invention is:

[0045] A method for reducing saline-alkali water to modern seawater suitable for aquaculture of various seafood by using microorganisms, comprising the following steps:

[0046] First, saline water pretreatment:

[0047] Pump saline water with a salinity of 15‰-30‰ (mass per thousand) into the breeding pond, and turn on the air compressor to fully mix the air and saline water to promote the precipitation of some substances for subsequent treatment. The pH value controlled in the saline water pretreatment process is: 7.3-7.5.

[0048] Second, microbial culture:

[0049] Purple halobacterium colonies that survive in coastal high-salinity and alkaline environments and purple halobacterium colonies that survive in plateau salt lakes were selected respectively. After multi-stage enrichment, the dominant halobacterium colonies of each were selected and mixed into purple halobacterium species for later use.

[0050] A mixture is obtained by mixing saline-alkali water from saline-alkali ground with a salinity of 10-20‰ (mass per thousand), saline-alkali water from a salt lake with a salinity of 180-200‰ (mass per thousand), and high-salinity alkaline soil containing some organic matter. The mixture is steamed at a temperature of 200-210°C, and then sealed in a light-transmitting incubator. The purple mixed halobacterium species selected in step 1 above are connected, and the air in the incubator is extracted. The mixture is cultured using sunlight. When the substrate shows purple color by visual observation, it can be expanded for standby use. The pH value controlled during inoculation and culture is: 8.3-8.5.

[0051] Third, reduction reaction:

[0052] The mixed purple salt bacteria cultured and expanded in the second step are inoculated into the saline water of the culture pond in the first step, and the waterwheel equipment is turned on for stirring for two hours and then turned off.

[0053] Carry out reduction reaction in a static water environment. After 5-10 days, when the water body shows reddish brown or dark brown color visually, add heterotrophic photosynthetic bacteria liquid, autotrophic photosynthetic bacteria liquid, and yeast powder. After reacting together for 5-10 days, add special aquaculture fish fertilizer preparation, turn on the oxygenation equipment 24 hours a day, react for 3 days, and control the pH value from 7.3-7.5 to 8.5-8.8.

[0054] Fourth, water quality monitoring and adjustment:

[0055] Monitor various indicators of the reduced saline-alkali water in real time, adjust the inoculation amount and type of microorganisms within the range of the first to third steps mentioned above according to the biological characteristics of seafood life, and detect the reduction and conversion process of the saline-alkali water to ensure that the water quality meets the requirements of various seafood farming.

[0056] Fifth, farmed seafood:

[0057] The restored saline-alkali water is used for seafood farming experiments; first, sea fish fry, shrimp, crab fry, and sea shellfish fry are tried. When more than 90% of them can survive after 48 hours, it proves that the restoration of seawater is successful.

[0058] The method of using microorganisms to reduce saline water to modern seawater suitable for breeding various seafoods comprises the following specific steps:

[0059] First, saline water pretreatment:

[0060] Pump 6677-6688 cubic meters of saline water with a salinity of 15‰-30‰ (mass per thousand) into the breeding pond, and turn on the air compressor to fully mix the air and saline water to promote the precipitation of some substances for subsequent treatment. The pH value controlled in the saline water pretreatment process is: 7.3-7.5.

[0061] Second, microbial culture:

[0062] Select purple halobacterium colonies that survive in coastal high-salinity and alkaline environments and purple halobacterium colonies that survive in plateau salt lakes respectively, and after multi-stage enrichment, select the superior halobacterium colonies of each, and mix the two into 500-510 grams of purple halobacterium species for later use.

[0063] A mixture is obtained by mixing 5000-5010 g of saline-alkali water from saline-alkali ground with a salinity of 10-20‰ (mass per thousand), 5000-5010 g of saline-alkali water from a salt lake with a salinity of 180-200‰ (mass per thousand), and 500-510 g of high-salinity alkaline soil containing some organic matter. The mixture is steamed at a temperature of 200-210°C, and then sealed in a light-transmitting incubator. The purple mixed halobacterium species selected in the first step is introduced into the incubator, and the air in the incubator is extracted. The mixture is cultured using sunlight. When the substrate shows purple color by visual observation, the mixture can be expanded for standby use. The pH value controlled during inoculation and culture is 8.3-8.5.

[0064] Third, reduction reaction:

[0065] The mixed purple halobacterium cultured and expanded in the second step is inoculated into the saline water of the breeding pond in the first step at 10-20 grams per cubic meter of saline water, and the waterwheel equipment is turned on for stirring for two hours and then turned off.

[0066] Carry out reduction reaction in a static water environment. After 5-10 days, when the water body appears reddish brown or brown by visual inspection, add 10-20 g of heterotrophic photosynthetic bacteria solution with a concentration of 3-5 billion / ml per cubic meter of water, add 10-20 g of autotrophic photosynthetic bacteria solution with a concentration of 3-5 billion / ml per cubic meter of water, and add 10-20 g of yeast powder with a specification of 10 billion / g per cubic meter of water. After 5-10 days of joint reaction, add 30-31 g of special aquaculture fish fertilizer preparation per cubic meter of water, turn on the oxygenation equipment 24 hours a day, react for 3 days, and control the pH value from 7.3-7.5 to 8.5-8.8.

[0067] Fourth, water quality monitoring and adjustment:

[0068] Monitor various indicators of the reduced saline-alkali water in real time, adjust the inoculation amount and type of microorganisms within the range of the first to third steps mentioned above according to the biological characteristics of seafood life, and detect the reduction and conversion process of the saline-alkali water to ensure that the water quality meets the requirements of various seafood farming.

[0069] Fifth, farmed seafood:

[0070] The restored saline-alkali water is used for seafood farming experiments; first, sea fish fry, shrimp, crab fry, and sea shellfish fry are tried. When more than 90% of them can survive after 48 hours, it proves that the restoration of seawater is successful.

[0071] The special aquaculture fish fertilizer preparation is connected, the oxygenation equipment is turned on 24 hours a day, and after three days of reaction, beneficial mixed concentrated algae liquid and beneficial zooplankton species are connected.

[0072] The re-introduction of beneficial mixed concentrated algae liquid and beneficial zooplankton species respectively refers to re-introduction of 50 grams of beneficial mixed concentrated algae liquid including triangular brown finger algae, marine green algae, and coralline algae per cubic meter of water, and re-introduction of 50 grams of live beneficial zooplankton including copepods such as Daphnia pulex and Daphnia pulex per cubic meter of water.

[0073] The pH value of the fourth water quality monitoring and adjustment and the fifth seafood farming two steps is 8.5-8.8.

[0074] The various seafoods include: golden drum fish, golden pomfret, pearl grouper, eastern star grouper, yellowtail snapper (yellow-legged sea bream), large oyster, East China Sea pearl, sea mullet (blackhead fish), mud fish, and seaweed.

[0075] The various seafoods described above do not require any domestication process and can be directly put into cultivation.

[0076] The special fish fertilizer for aquaculture is prepared by mixing the following raw materials in the following weight ratios:

[0077] The seed powder of Clostridium thermocellum is 0.1 to 0.2 parts;

[0078] The seed powders of Actinomyces melanocyles, A. roseus and A. cellulose are 0.1 to 0.2 parts respectively;

[0079] The seed powders of Aspergillus oryzae, Rhizopus, and Trichoderma viride are 0.1 to 0.2 parts respectively;

[0080] 18-20 parts of wheat bran mixture: Mix wheat bran and sterile water in a weight ratio of 1:1 and set aside;

[0081] Saccharomyces and Geotrichum candidum seed powders are 0.1 to 0.2 parts respectively;

[0082] Lactic acid bacteria (LAB. Lactic acid bacteria) and heterolactic acid fermentation bacteria liquid are 0.5-0.6 parts respectively;

[0083] The seed solution of photoautotrophic or chemoautotrophic photosynthetic bacteria and heterotrophic purple nitrogen-fixing photosynthetic bacteria are 0.5 to 0.6 parts respectively;

[0084] Mix 16-26 parts of fungus nutrient solution: 1-3 parts of potassium dihydrogen phosphate solution, 1-1.5 parts of calcium chloride, 1-1.5 parts of urea, and 5-7 parts of sugars, and add the mixture and sterile water in a weight ratio of 1:2 to dissolve the mixture in sterile water for later use.

[0085] The preparation of the special fish fertilizer for aquaculture comprises the following steps (parts involved are by weight):

[0086] ⑴Material preparation:

[0087] ① The seed powder of Thermocellulosic Clostridium is 0.1-0.2 parts;

[0088] ② The seed powders of black-red spiral actinomycetes, rose-colored actinomycetes, and fiber actinomycetes are 0.1-0.2 parts respectively;

[0089] ③ The seed powders of Aspergillus oryzae, Rhizopus, and Trichoderma viride are 0.1 to 0.2 parts respectively;

[0090] ④18-20 parts of wheat bran mixture: wheat bran and sterile water are added to sterile water in a weight ratio of 1:1, mixed well and set aside;

[0091] ⑤ Yeast and Geotrichum candidum seed powder are 0.1-0.2 parts respectively;

[0092] ⑥ Lactic acid bacteria and heterolactic acid fermentation bacteria liquid are 0.5-0.6 parts respectively;

[0093] ⑦ The seed solution of photoautotrophic or chemoautotrophic photosynthetic bacteria and heterotrophic purple nitrogen-fixing photosynthetic bacteria are 0.5-0.6 parts respectively;

[0094] ⑧ 16-26 parts of fungus nutrient solution: 1-3 parts of potassium dihydrogen phosphate solution, 1-1.5 parts of calcium chloride, 1-1.5 parts of urea, and 5-7 parts of sugars are mixed, and the mixture is added to sterile water in a weight ratio of 1:2 and dissolved in sterile water for later use.

[0095] ⑵Production:

[0096] First, mix ①, ②, and ③ of the above-mentioned preparations, then add ④ of the above-mentioned preparations and mix well, and ferment on the slant for 5 days, turning over once in the morning and evening every day; after 5 days, take a sample and dissolve it in water. When the pH value of the solution reaches 9.0, add the fermented material and sterile water in a weight ratio of 1:3.5 and mix well. If the pH value does not reach 9.0, continue to ferment for 2 to 3 days; add ⑤ and ⑥ of the above-mentioned preparations and mix well, and then aerate and ferment for 10 days; after 10 days, when the pH value is tested to be 3 to 4, filter out the bacterial residue, take the bacterial liquid and add ⑧ of the above-mentioned preparations, mix well, and ferment statically for 2 days; then add ⑦ of the above-mentioned preparations and mix well, and then ferment statically for 3 days; after 3 days, package it in barrels to obtain the finished product.

[0097] Application of a special fish fertilizer for aquaculture in the microbial treatment of saline-alkali water to modern seawater aquaculture of various seafood:

[0098] The special fish fertilizer for aquaculture is prepared by mixing the following raw materials in the following weight ratios:

[0099] The seed powder of Clostridium thermocellum is 0.1 to 0.2 parts;

[0100] The seed powders of Actinomyces melanocyles, A. roseus and A. cellulose are 0.1 to 0.2 parts respectively;

[0101] The seed powders of Aspergillus oryzae, Rhizopus, and Trichoderma viride are 0.1 to 0.2 parts respectively;

[0102] 18-20 parts of wheat bran mixture: Mix wheat bran and sterile water in a weight ratio of 1:1 and set aside;

[0103] Saccharomyces and Geotrichum candidum seed powders are 0.1 to 0.2 parts respectively;

[0104] Lactic acid bacteria (LAB. Lactic acid bacteria) and heterolactic acid fermentation bacteria liquid are 0.5-0.6 parts respectively;

[0105] The seed solution of photoautotrophic or chemoautotrophic photosynthetic bacteria and heterotrophic purple nitrogen-fixing photosynthetic bacteria are 0.5 to 0.6 parts respectively;

[0106] Mix 16-26 parts of fungus nutrient solution: 1-3 parts of potassium dihydrogen phosphate solution, 1-1.5 parts of calcium chloride, 1-1.5 parts of urea, and 5-7 parts of sugars, and add the mixture and sterile water in a weight ratio of 1:2 to dissolve the mixture in sterile water for later use.

[0107] The preparation of the special fish fertilizer for aquaculture comprises the following steps:

[0108] ⑴Material preparation:

[0109] ① The seed powder of Thermocellulosic Clostridium is 0.1-0.2 parts;

[0110] ② The seed powders of black-red spiral actinomycetes, rose-colored actinomycetes, and fiber actinomycetes are 0.1-0.2 parts respectively;

[0111] ③ The seed powders of Aspergillus oryzae, Rhizopus, and Trichoderma viride are 0.1 to 0.2 parts respectively;

[0112] ④18-20 parts of wheat bran mixture: wheat bran and sterile water are added to sterile water in a weight ratio of 1:1, mixed well and set aside;

[0113] ⑤ Yeast and Geotrichum candidum seed powder are 0.1-0.2 parts respectively;

[0114] ⑥ Lactic acid bacteria and heterolactic acid fermentation bacteria liquid are 0.5-0.6 parts respectively;

[0115] ⑦ The seed solution of photoautotrophic or chemoautotrophic photosynthetic bacteria and heterotrophic purple nitrogen-fixing photosynthetic bacteria are 0.5-0.6 parts respectively;

[0116] ⑧ 16-26 parts of fungus nutrient solution: 1-3 parts of potassium dihydrogen phosphate solution, 1-1.5 parts of calcium chloride, 1-1.5 parts of urea, and 5-7 parts of sugars are mixed, and the mixture is added to sterile water in a weight ratio of 1:2 and dissolved in sterile water for later use.

[0117] ⑵Production:

[0118] First, mix ①, ②, and ③ of the above-mentioned preparations, then add ④ of the above-mentioned preparations and mix well, and ferment on the slant for 5 days, turning over once in the morning and evening every day; after 5 days, take a sample and dissolve it in water. When the pH value of the solution reaches 9.0, add the fermented material and sterile water in a weight ratio of 1:3.5 and mix well. If the pH value does not reach 9.0, continue to ferment for 2 to 3 days; add ⑤ and ⑥ of the above-mentioned preparations and mix well, and then aerate and ferment for 10 days; after 10 days, when the pH value is tested to be 3 to 4, filter out the bacterial residue, take the bacterial liquid and add ⑧ of the above-mentioned preparations, mix well, and ferment statically for 2 days; then add ⑦ of the above-mentioned preparations and mix well, and then ferment statically for 3 days; after 3 days, package it in barrels to obtain the finished product.

[0119] Beneficial effects of the present invention:

[0120] First, breaking the technical conventions, real seafood can be cultivated on demand in the saline water of deserts and Gobi deserts. The real seafood includes: golden drum, golden pomfret, pearl grouper, eastern star grouper, yellowtail sea bream (yellow-legged sea bream), giant oysters, East China Sea pearls, sea mullet (blackhead fish), mud fish, and seaweed.

[0121] Second, it can achieve zero pollution and zero emissions, which can meet the necessary conditions of not allowing the indiscriminate discharge of aquaculture water in the desert and Gobi, avoiding the huge risk of environmental pollution, and complying with the country's long-term energy conservation and emission reduction strategy requirements.

[0122] Second, it has been proven that the cost of developing and implementing the present invention is relatively low. Compared with the high cost of the prior art, which costs 264,250 yuan to prepare 10,000 cubic meters of 10-12‰ seawater, the cost of preparing 10,000 cubic meters of water in the present invention is only 675 yuan, so it has huge economic benefits.

[0123] Fourth, the reality that the ocean, especially the offshore, is polluted by industry is inevitable. Finding authentic seafood cultivated in pristine seawater is a technical challenge in this field. The modern seawater restored by the present invention has broad social significance and social benefits.

[0124] Fifth, the present invention always relies on microorganisms to play a role, which can effectively inhibit the reproduction of pathogenic bacteria in aquatic animals, solve the problem of aquaculture pollution, enhance the disease resistance of animals and maintain the ecological balance of water bodies. It is not only a pure natural and green biological agent, but also an ideal substitute for antibiotics, and can effectively improve the output, quality, feed conversion rate and economic benefits of aquatic products.

[0125] ⑴ In aquaculture, there is no need to add fertilizers and chemicals to the pond. Just add a small amount of river water or sea water to the aquaculture pond regularly, and then add the microbial preparation of the present invention to obtain the amount of nutrients required by the microorganisms in the aquaculture pond, cultivate and reproduce abundant primary live bait and microbial species, form a close circulation ecological environment and food chain in the water body, prevent "pond overturning" and "floating head", and greatly reduce the mortality rate.

[0126] ⑵Can quickly degrade organic matter and harmful substances. Through the massive reproduction and metabolites of microorganisms in the product, it can quickly and effectively degrade organic matter in the water, decompose ammonia nitrogen, nitrite, sulfide, phosphorus and other harmful substances, and use pure microbial methods to protect "life" (the life activities of microorganisms themselves) with "life" (the health of fish and shrimp).

[0127] ⑶ It can purify and improve water quality. Through the reproduction of functional microorganisms in the product and their metabolites, it can eliminate or greatly reduce bottom sedimentation, purify and improve water quality, and maintain the ecological balance of the water environment.

[0128] ⑷ It can effectively inhibit pathogens. The high-efficiency and high-activity microorganisms in the product multiply in large quantities (theoretical number of reproduction in 24 hours can reach 2 72 The beneficial bacteria can inhibit the growth and reproduction of pathogenic microorganisms, thus enhancing the immunity of aquatic animals and reducing the mortality rate by more than 90%.

[0129] ⑸Can significantly improve economic benefits. The cost performance and input-output ratio are above 1:10. BRIEF DESCRIPTION OF THE DRAWINGS

[0130] 1. Data of saline-alkali water testing before treatment in Qiemo County, Xinjiang:

[0131] Figure 1 The data is from September 24 to 26, 2023.

[0132] Figure 2The data is from September 26 to 27, 2023.

[0133] Figure 3 The data is from September 29 to October 1, 2023.

[0134] Figure 4 The data is from October 1 to 3, 2023.

[0135] Figure 5 The data is from October 3 to 4, 2023.

[0136] 2. Data of saline-alkali water treated in Kizilsu Kirgiz Autonomous Prefecture, Xinjiang:

[0137] Figure 6 Data is as of May 31, 2024.

[0138] Figure 7 The data is as of May 28, 2024.

[0139] 3. Proof of practical application of the applicant’s invention application:

[0140] Figure 8 It is the technical application in Hongqi Farm of the Third Division of Xinjiang Production and Construction Corps.

[0141] Fig. 9 It is the technology application in Tatirang Township, Qiemo County, Xinjiang.

[0142] Fig.10 It is the technology application in Halajun Township, Atushi City, Kezhou Prefecture, Xinjiang.

[0143] 4. The results of breeding various seafood in Halajun Township, Atushi City, Kezhou Prefecture, Xinjiang:

[0144] Fig.11 This is a picture of the effect of breeding yellowtail catfish (yellow-legged catfish).

[0145] Fig.12 This is a picture of the effect of breeding golden drum fish.

[0146] Fig.13 This is a picture of the effect of breeding golden pomfret.

[0147] Fig.14 This is a picture of the effect of farming sea mullet (blackhead fish).

[0148] Fig.15 This is a rendering of pearl grouper being farmed.

[0149] Fig.16 This is the effect picture of cultured East China Sea pearls.

[0150] Fig.17 This is the effect picture of oyster farming.

[0151] Fig.18 This is the effect picture of breeding large pearl grouper.

[0152] Fig.19 This is the effect picture of breeding mud fish.

[0153] Fig. 20 This is the effect picture of cultivating seaweed.

[0154] The test data involved in 1-4 above are all tested using the "Charoen Pokphand Water Quality Analysis Kit" produced by Charoen Pokphand Group. The water quality analysis kit includes: shrimp set: ammonia nitrogen, nitrite, pH, total alkali, calcium and magnesium ions, potassium salt, etc. DETAILED DESCRIPTION

[0155] The inventor has been the technical director of the applicant Guangxi Sansheng Biotechnology Co., Ltd. and applied for a patent on April 25, 2009 entitled "A special fish fertilizer for aquaculture and its preparation method". On June 13, 2012, he was granted an invention patent by the State Intellectual Property Office (patent number ZL200910137979.6).

[0156] Xinjiang Shishixian Aquatic Products Co., Ltd. has established a seafood breeding base in Hongqi Farm, the Third Division of Xinjiang Production and Construction Corps. It has applied the technology and microbial preparations of Guangxi Sansheng Biotechnology Co., Ltd., "a method of using microorganisms to reduce saline-alkali water to modern seawater suitable for breeding various seafood", to seafood breeding, and has used it as a core technology and important breeding supplies (see Appendix). Figure 8 ).

[0157] The seafood breeding base established by Qiemo Shishixian Aquatic Products Co., Ltd. in Tatirang Township, Qiemo County, Xinjiang, has adopted the technology and microbial preparations of Guangxi Sansheng Biotechnology Co., Ltd., "a method of using microorganisms to reduce saline-alkali water to modern seawater suitable for breeding various seafood", as the core technology and important breeding supplies for seafood breeding (see Appendix). Fig. 9 ).

[0158] The seafood breeding base established by Xinjiang Kezhou Zhanyu Aquatic Products Co., Ltd. in Halajun Township, Atushi City, Kezhou, adopts the technology and microbial preparations of Guangxi Sansheng Biotechnology Co., Ltd., "a method of using microorganisms to reduce saline water to modern seawater suitable for breeding various seafood", and regards it as the core technology of seafood breeding and an important breeding product (attached Fig.10 ).

[0159] The active ingredients involved in the present invention are:

[0160] Thermocellus Bacillus, Latin name: Clostridium thermocellum;

[0161] Description: This fungus is suitable for high temperature fermentation and produces polycellulose enzyme complex in the fermented material, which can dissolve various fibers and lignin, and its solution contains a small amount of ethanol.

[0162] Actinomyces melanocyles, rose-colored actinomycetes, A. roseus, and cellulose actinomycetes, A. cellulose;

[0163] Description: These three types of bacteria have basically the same functions and properties. They can decompose organic matter and produce extracellular enzymes to decompose protein, cellulose, lignin, and chitin. Therefore, this property can be used to deal with substances with chitin cell walls. The chitinase secreted by this bacterial group can dissolve a variety of pathogenic hyphae.

[0164] Aspergillus oryzae, its Latin name: Aspergillus oryzae;

[0165] Description: This fungus can produce saccharifying mold, fiber mold and phytic acid mold in the fermented materials. It can degrade straight-chain and branched chain powder, crude fiber and phytic acid into various fine molecules and free substances, and degrade large molecular proteins into peptones, polypeptides and various amino acids.

[0166] Rhizopus, its Latin name: Rhizopus;

[0167] Description: This fungus is a fungus that helps mold fermentation. The secretions it produces can help catalyze decomposition during mold fermentation.

[0168] Trichoderma viride:

[0169] Description: This fungus is a kind of glucan hydrolyzing mold (enzyme). It has strong catalytic effect on the hydrolysis of cellulose and lignin.

[0170] Yeast, its Latin name: Saccharomyces, Geotrichumcandidum, its Latin name: Geotrichumcandidum;

[0171] Description: These two types of bacteria have basically the same functions and properties. They can utilize nutrients in the fermentation substrate to synthesize their own proteins and B vitamins, and convert various organic wastewaters into bacterial proteins, vitamins, various enzymes, hormones, etc.

[0172] Lactic acid bacteria, its Latin name: LAB.Lactic acid bacteria;

[0173] Description: This bacterium can decompose carbohydrates in materials to form lactic acid, playing a balancing role in multi-bacteria fermentation.

[0174] Heterolactic fermentation bacteria:

[0175] Description: This bacterium can secrete acetic acid, succinic acid, hydrogen and carbon dioxide into the material during multi-bacteria fermentation, provide nutrition for other bacteria, and play a coordinating role in purifying the environment.

[0176] Photoautotrophic or chemoautotrophic photosynthetic bacteria,

[0177] Description: This bacterium absorbs inorganic substances as its own nutrition, transforms and utilizes ammonia nitrogen, nitrite, and hydrogen sulfide in the water, effectively improves water quality, balances pH value, and accelerates the circulation of nutrients in the water.

[0178] Heterotrophic purple nitrogen-fixing photosynthetic bacteria,

[0179] Description: This bacterium uses light as energy and various organic matter as the main carbon source. It can assimilate various organic matter to form its own substances. It can absorb and assimilate a large number of fine molecular organic matter in the water body. It prevents other harmful bacteria from converting organic matter into harmful subspecies, which is of great significance in treating sewage and purifying the environment.

[0180] The process of implementing the technology of the present invention:

[0181] 1. Successfully used underground saline-alkali water and underground brine mines around the desert in Ruoqiang County, Bayinguoleng Mongol Autonomous Prefecture, Xinjiang Uygur Autonomous Region to restore seawater aquaculture.

[0182] 2. In Qiemo County, located in the heart of the Taklimakan Desert, marine fish have entered the desert and turned it into their home. 100,000 high-quality seafood such as pearl oysters, mullets, groupers and multi-scaled mullets have been successfully raised in the desert, with a survival rate of more than 99%. This is a major initiative in my country to cultivate seafood in the desert after the Gobi Desert. At the "Shishixian" aquatic seafood breeding base in Tatirang Town, Qiemo County, the outdoor temperature reached 43°C and the heat wave rolled. The staff started the aerator and fully automatic feeding machine in the fish pond by controlling the power supply. In the fish pond deep in the desert, water splashed out with the aerator, and the fish jumped and jumped for food. The breeding base has a total area of ​​20 mu, including two fish ponds, a breeding pool and a standardized seafood production workshop. There are 100,000 precious species such as golden pomfret, grouper, mullet, mudfish, yellow-legged mullet and multi-scaled mullet in the fish pond. While breeding marine fish, the base also put 2 million pearl oyster seedlings and some oysters and other aquatic species into the fish pond for trial breeding. The first batch of marine fish will be put on the market in large quantities at the end of this year and will be sold all over the country. The breeding farm in the desert was successful in the first half of 2024, and now it continues to release seedlings.

[0183] 3. Beida Lake is located in the Inner Mongolia grassland, with pristine ecology, no pollution and beautiful environment. Beida Lake natural lake salt is definitely a gift from nature. Since lake salt is formed in the natural environment, its crystals contain rich trace elements, such as calcium, magnesium, iron and potassium. The composition of these trace elements is an important "fingerprint" of lake salt characteristics and plays a key role in human health.

[0184] The Lop Nur potash deposit (super-large potash deposit, Xinjiang Lop Nur dry salt lake brine potash deposit) is the largest sulfate-type brine potash deposit in China. It is located in the eastern end of the Tarim Basin in Xinjiang Uygur Autonomous Region, in the northern area of ​​Lop Nur. Specifically, it is located in Ruoqiang County, Bayinguoleng Mongol Autonomous Prefecture, Xinjiang Uygur Autonomous Region, about 450 kilometers away from Korla City in the west and about 300 kilometers away from Bushan County in the north.

[0185] The above two places, one extracts salt and the other extracts potassium salt. The wastewater after extracting the two salts is used to successfully cultivate various seafood after reducing seawater using the present invention.

[0186] 4. Underground saline water from other places or the saline water from the drainage ditches of all farms in Xinjiang is also collected and restored to modern seawater aquaculture seafood.

[0187] 5. Tuzisuget Salt Lake is about 18 kilometers long, 2-4 kilometers wide, and covers an area of ​​about 50 square kilometers. The thickness of the salt layer is 0.5-0.8 meters; the crystal size is between 5mmx8mm and 2mmx4mm. The salt particles are gray-green, containing 94% sodium chloride, 0.39% calcium, 0.01% magnesium, 1.02% carbonate, 2.96% underwater dissolved matter, and 1.1% water. The salt reserves are 13.2 million tons, and the content of mirabilite is 1.2 million tons. This is the location of Kezhou Zhanyu Company, and now the farmed seafood is growing very well and successfully.

[0188] The following is the detection of saline water before treatment in various places:

[0189] A:

[0190] 1. Testing of salt mine soil in Tatirang Township, Qiemo County, Bayingolin Mine, Xinjiang on July 19, 2024:

[0191] Salinity: 275‰

[0192] pH: 9.5

[0193] Nitrite:0.

[0194] Ammonia: 0

[0195] Calcium: 100

[0196] Magnesium: 717

[0197] Potassium salt 275

[0198] Total alkalinity: 675.

[0199] 2. On July 19, 2024, the saline-alkali sandy soil in the breeding base of Tatirang Township, Qiemo County, Bayingolin Mongol Autonomous Prefecture, Xinjiang was tested:

[0200] Salinity 10‰

[0201] pH 8.7

[0202] Nitrite 0.07

[0203] Ammonia nitrogen 0

[0204] Calcium: 360

[0205] Magnesium: 250

[0206] Potassium: 161

[0207] Total alkali: 304.

[0208] 3. On July 19, 2024, the saline-alkali desert groundwater at the Tatirang Township breeding base of Shishixian Aquatic Products Co., Ltd. in Qiemo County, Bayingolin Mongol Autonomous Prefecture, Xinjiang was tested:

[0209] Salinity: 6‰

[0210] pH: 6.8

[0211] Nitrite: 0

[0212] Ammonia: 0.2

[0213] Hydrogen sulfide: 1.5mg / L

[0214] Potassium: 155

[0215] Calcium: 160

[0216] Magnesium: 97

[0217] Total base: 104.

[0218] 4. Water quality test records of the breeding ponds of the breeding base of Shishixian Aquatic Products Co., Ltd. in Tatirang Township, Qiemo County, Bayingolin Mongol Autonomous Prefecture, Xinjiang:

[0219] Pond No. 1:

[0220] Salinity: 22‰

[0221] pH: 8.6

[0222] Ammonia: 0

[0223] Nitrite: 0.01

[0224] Calcium: 170

[0225] Magnesium: 450

[0226] Potash: 193

[0227] Total alkalinity content: 350

[0228] Bicarbonate Alkalinity: 250

[0229] Breeding Pond No. 2:

[0230] Salinity: 18.5‰

[0231] pH: 8.5

[0232] Ammonia: 0

[0233] Nitrite: 0

[0234] Calcium: 160

[0235] Magnesium: 438

[0236] Potassium salt: 156

[0237] Total alkalinity content: 370

[0238] Bicarbonate alkalinity: 270.

[0239] B:

[0240] 1. Testing of salt lake water in Halajun Township, Atushi City, Kezhou Prefecture, Xinjiang on July 21, 2024:

[0241] Salinity: 115‰

[0242] pH: 8.9

[0243] Nitrite 0.07

[0244] Ammonia: 0

[0245] Calcium: 100

[0246] Magnesium: 717

[0247] Potassium salt: 75

[0248] Total alkalinity: 675.

[0249] 2. On July 21, 2024, the Gobi Desert soil of the breeding base of Xinjiang Kezhou Zhanyu Aquatic Products Co., Ltd. in Halajun Township, Atushi City, Kezhou Prefecture was tested:

[0250] Salinity: 10‰

[0251] pH: 9.6

[0252] Nitrite: 0.01

[0253] Ammonia nitrogen: 0

[0254] Calcium: 560

[0255] Magnesium: 250

[0256] Potassium: 261.

[0257] 3. On July 22, 2024, the groundwater in the Gobi Desert of the Halajun Township breeding base of Kezhou Zhanyu Aquatic Products Co., Ltd. in Atushi City, Kezhou Prefecture, Xinjiang was tested:

[0258] Salinity: 10‰

[0259] pH: 8.7

[0260] Nitrite: 0

[0261] Ammonia: 0

[0262] Hydrogen sulfide: 0

[0263] Potassium: 142

[0264] Calcium: 180

[0265] Magnesium: 255

[0266] Total alkali: 586.

[0267] 4. The water quality test record of the breeding pond No. 1 breeding base in Halajun Township, Kezhou Zhanyu Aquatic Products Co., Ltd., Atushi City, Kezhou Prefecture, Xinjiang, on July 23, 2024:

[0268] Area A Datang (#3)

[0269] Salinity: 20‰

[0270] PH value: 8.5

[0271] Nitrite concentration: 0.01

[0272] Ammonia content: 0

[0273] Calcium ion concentration: 160

[0274] Magnesium ion concentration: 389

[0275] Potassium salt content: 148

[0276] Total alkalinity content: 280

[0277] Bicarbonate alkalinity: 180.

[0278] Area B Datang (#4)

[0279] Salinity: 21‰

[0280] PH value: 8.6

[0281] Nitrite concentration: 0

[0282] Ammonia content: 0

[0283] Calcium ion concentration: 180

[0284] Magnesium ion concentration: 401

[0285] Potassium salt content: 155

[0286] Total alkalinity content: 270

[0287] Bicarbonate alkalinity: 170.

[0288] Area C Datang (#5)

[0289] Salinity: 20.5‰

[0290] PH value: 8.5

[0291] Nitrite concentration: 0.05

[0292] Ammonia content: 0

[0293] Calcium ion concentration: 160

[0294] Magnesium ion concentration: 437

[0295] Potassium salt content: 155

[0296] Total alkalinity content: 270

[0297] Bicarbonate alkalinity: 170.

[0298] C:

[0299] 1. The water quality record of the well water at the base of Xinjiang Shishixian Aquatic Products Co., Ltd. at Hongqi Farm, the Third Division of Xinjiang Production and Construction Corps, on July 6, 2022:

[0300] Salinity: 8‰

[0301] Nitrite 0

[0302] pH 8.5

[0303] Ammonia 0.2

[0304] Potassium: 55

[0305] Calcium 100

[0306] Magnesium: 480

[0307] Total alkalinity: 504.

[0308] 2. Xinjiang Shishixian Aquatic Products Co., Ltd. has saline-alkali water in the drainage ditch next to the breeding base of Hongqi Farm. The water quality record of the drainage ditch was tested on July 6, 2022:

[0309] Salinity: 23‰

[0310] Nitrite: 0.25

[0311] pH8.9

[0312] Ammonia 0.2

[0313] Potassium 242

[0314] Calcium 280

[0315] Magnesium: 560

[0316] Total alkalinity: 650.

[0317] 3. Water quality test records of the breeding ponds and factory breeding greenhouses of Xinjiang Shishixian Aquatic Products Co., Ltd. at the breeding base of Hongqi Farm, the Third Division of Xinjiang Production and Construction Corps on May 3, 2023:

[0318] Pond No. 1:

[0319] Salinity: 20‰

[0320] pH: 8.6

[0321] Ammonia: 0

[0322] Nitrite: 0.01

[0323] Calcium: 170

[0324] Magnesium: 450

[0325] Potassium salt: 173

[0326] Total alkalinity content: 350

[0327] Bicarbonate alkalinity: 250.

[0328] Breeding Pond No. 2:

[0329] Salinity: 20.5‰

[0330] pH: 8.5

[0331] Ammonia: 0

[0332] Nitrite: 0.05

[0333] Calcium: 160

[0334] Magnesium: 338

[0335] Potassium salt: 156

[0336] Total alkalinity content: 370

[0337] Bicarbonate alkalinity: 270.

[0338] Breeding Pond No. 2:

[0339] Salinity: 20.5‰

[0340] pH: 8.5

[0341] Ammonia: 0

[0342] Nitrite: 0.05

[0343] Calcium: 160

[0344] Magnesium: 338

[0345] Potassium salt: 156

[0346] Total alkalinity content: 370

[0347] Bicarbonate alkalinity: 270.

[0348] Breeding shed No. 1:

[0349] Salinity: 19‰

[0350] pH: 8.4

[0351] Ammonia: 0

[0352] Nitrite: 0.05

[0353] Calcium: 160

[0354] Magnesium: 430

[0355] Potassium salt: 183

[0356] Total alkalinity content: 370

[0357] Bicarbonate alkalinity: 260.

[0358] Breeding shed No. 2:

[0359] Salinity: 19.5‰

[0360] pH: 8.7

[0361] Ammonia: 0

[0362] Nitrite: 0.03

[0363] Calcium: 150

[0364] Magnesium: 385

[0365] Potassium salt: 158

[0366] Total alkalinity content: 360

[0367] Bicarbonate alkalinity: 260.

[0368] D:

[0369] See the attached for the test data of saline-alkali water before treatment in Qiemo County, Xinjiang. Figure 1-5 .

[0370] The untreated saline water from the above AD locations was used as the reduced water source in the following Examples 1-2:

[0371] Embodiment 1:

[0372] A method for reducing saline-alkali water to modern seawater suitable for aquaculture of various seafood by using microorganisms, comprising the following steps:

[0373] First, saline water pretreatment:

[0374] Pump saline water with a salinity of 15‰ (mass per thousand) into the breeding pond, and turn on the air compressor to fully mix the air and saline water to promote the precipitation of some substances for subsequent treatment. The pH value controlled in the saline water pretreatment process is: 7.3.

[0375] Second, microbial culture:

[0376] Purple halobacterium colonies that survive in coastal high-salinity and alkaline environments and purple halobacterium colonies that survive in plateau salt lakes were selected respectively. After multi-stage enrichment, the dominant halobacterium colonies of each were selected and mixed into purple halobacterium species for later use.

[0377] A mixture is obtained by mixing saline-alkali water from saline-alkali ground with a salinity of 10‰ (mass per thousand), saline-alkali water from a salt lake with a salinity of 180‰ (mass per thousand), and high-salinity alkaline soil containing some organic matter. The mixture is steamed at 200°C, sealed in a light-transmitting incubator, and the purple mixed halobacterium species selected in the above step 1 are connected. The air in the incubator is extracted, and the culture is carried out using sunlight. When the matrix shows purple color by visual observation, it can be expanded for use. The pH value controlled during inoculation and culture is: 8.3.

[0378] Third, reduction reaction:

[0379] The mixed purple salt bacteria cultured and expanded in the second step are inoculated into the saline water of the culture pond in the first step, and the waterwheel equipment is turned on for stirring for two hours and then turned off.

[0380] Carry out reduction reaction in a static water environment. After 5 days, when the water body shows reddish brown or dark brown color visually, add heterotrophic photosynthetic bacteria liquid, autotrophic photosynthetic bacteria liquid, and yeast powder. After reacting together for 5 days, add special aquaculture fish fertilizer preparation, turn on the oxygenation equipment 24 hours a day, react for 3 days, and control the pH value from 7.3 to 8.5.

[0381] Fourth, water quality monitoring and adjustment:

[0382] Monitor various indicators of the reduced saline-alkali water in real time, adjust the inoculation amount and type of microorganisms within the range of the first to third steps mentioned above according to the biological characteristics of seafood life, and detect the reduction and conversion process of the saline-alkali water to ensure that the water quality meets the requirements of various seafood farming.

[0383] Fifth, farmed seafood:

[0384] The restored saline-alkali water is used for seafood farming experiments; first, sea fish fry, shrimp, crab fry, and sea shellfish fry are tried. When more than 90% of them can survive after 48 hours, it proves that the restoration of seawater is successful.

[0385] A method for reducing saline-alkali water to modern seawater suitable for aquaculture of various seafood by using microorganisms, comprising the following specific steps:

[0386] First, saline water pretreatment:

[0387] 6677 cubic meters of saline water with a salinity of 15‰ (mass per thousand) was pumped into the breeding pond, and the air compressor was turned on to fully mix the air and the saline water to promote the precipitation of some substances for subsequent treatment. The pH value controlled in the saline water pretreatment process was: 7.3.

[0388] Second, microbial culture:

[0389] Select the purple halobacterium colonies that survive in the coastal high-salinity environment and the purple halobacterium colonies that survive in the plateau salt lake. After multi-stage enrichment, select the dominant halobacterium colonies of each and mix them into 500 grams of purple halobacterium species for standby use.

[0390] A mixture is obtained by mixing 5000 g of saline-alkali water from saline-alkali ground with a salinity of 10‰ (mass per thousand), 5000 g of saline-alkali water from a salt lake with a salinity of 180‰ (mass per thousand), and 500 g of high-salinity alkaline soil containing some organic matter. The mixture is steamed at 200°C, sealed in a light-transmitting incubator, and the purple mixed halobacterium species selected in the first step is inoculated. The air in the incubator is extracted and cultured using sunlight. When the matrix shows purple color by visual observation, it can be expanded for use. The pH value controlled during inoculation and culture is: 8.3.

[0391] Third, reduction reaction:

[0392] The mixed purple halobacterium cultured and expanded in the second step is inoculated into the saline water of the breeding pond in the first step at 10 grams per cubic meter of saline water, and the waterwheel equipment is turned on for stirring for two hours and then turned off.

[0393] Carry out reduction reaction in a static water environment. After 5 days, when the water body appears reddish brown or brown by visual inspection, add 10 g of heterotrophic photosynthetic bacteria solution with a concentration of 3 billion / ml per cubic meter of water, add 10 g of autotrophic photosynthetic bacteria solution with a concentration of 3 billion / ml per cubic meter of water, and add 10 g of yeast powder with a specification of 10 billion / g per cubic meter of water. After 5 days of joint reaction, add 30 g of special aquaculture fish fertilizer preparation per cubic meter of water, turn on the oxygenation equipment 24 hours a day, react for 3 days, and control the pH value from 7.3 to 8.5.

[0394] Fourth, water quality monitoring and adjustment:

[0395] Monitor various indicators of the reduced saline-alkali water in real time, adjust the inoculation amount and type of microorganisms within the range of the first to third steps according to the biological characteristics of seafood life, and detect the reduction and conversion process of the saline-alkali water to ensure that the water quality meets the requirements of various seafood farming;

[0396] Fifth, farmed seafood:

[0397] The restored saline-alkali water is used for seafood farming experiments; first, sea fish fry, shrimp, crab fry, and sea shellfish fry are tried. When more than 90% of them can survive after 48 hours, it proves that the restoration of seawater is successful.

[0398] The special aquaculture fish fertilizer preparation is connected, the oxygenation equipment is turned on 24 hours a day, and after three days of reaction, beneficial mixed concentrated algae liquid and beneficial zooplankton species are connected.

[0399] The re-introduction of beneficial mixed concentrated algae liquid and beneficial zooplankton species respectively refers to re-introduction of 50 grams of beneficial mixed concentrated algae liquid including triangular brown finger algae, marine green algae, and coralline algae per cubic meter of water, and re-introduction of 50 grams of live beneficial zooplankton including copepods such as Daphnia pulex and Daphnia pulex per cubic meter of water.

[0400] The pH value of the fourth water quality monitoring and adjustment and the fifth seafood farming two steps is 8.5.

[0401] The various seafoods include: golden drum fish, golden pomfret, pearl grouper, eastern star grouper, yellowtail snapper (yellow-legged sea bream), large oyster, East China Sea pearl, sea mullet (blackhead fish), mud fish, and seaweed.

[0402] The various seafoods described above do not require any domestication process and can be directly put into cultivation.

[0403] The method of using microorganisms to reduce saline-alkali water to modern seawater suitable for breeding various seafood, the special fish fertilizer for aquaculture is mixed with the following raw materials in weight ratio:

[0404] The seed powder of Clostridium thermocellum is: 0.1kg.

[0405] The seed powders of Actinomyces melanocyles, A.roseus and A.cellulose are 0.1kg respectively.

[0406] The seed powders of Aspergillus oryzae, Rhizopus, and Trichoderma viride are 0.1 kg respectively.

[0407] 18kg wheat bran mixture: Mix wheat bran and sterile water in a weight ratio of 1:1 and set aside.

[0408] The seed powder of Saccharomyces and Geotrichum candidum are 0.1kg respectively.

[0409] The lactic acid bacteria (LAB. Lactic acid bacteria) and heterolactic acid fermentation bacterial liquid are respectively: 0.5kg.

[0410] The seed solution of photoautotrophic or chemoautotrophic photosynthetic bacteria and heterotrophic purple nitrogen-fixing photosynthetic bacteria is 0.5kg respectively.

[0411] 16kg fungus nutrient solution: 1kg potassium dihydrogen phosphate solution, 1kg calcium chloride, 1kg urea, and 5kg sugars are mixed, and the mixture is added to sterile water in a weight ratio of 1:2 to dissolve for later use.

[0412] The method of using microorganisms to reduce saline-alkali water to modern seawater suitable for breeding various seafoods is characterized in that the preparation of the special fish fertilizer for aquaculture comprises the following steps (the parts involved are by weight):

[0413] (1) Preparation:

[0414] ① The seed powder of Thermocellulosic Clostridium is: 0.1kg.

[0415] ② The seed powder of black-red spiral actinomycetes, rose-colored actinomycetes, and fiber actinomycetes are: 0.1kg respectively.

[0416] ③ The seed powders of Aspergillus oryzae, Rhizopus, and Trichoderma viride are 0.1kg respectively.

[0417] ④18kg wheat bran mixture: Add wheat bran and sterile water in a weight ratio of 1:1 to sterile water and mix well for later use;

[0418] ⑤ The yeast and Geotrichum candidum seed powders are 0.1kg respectively.

[0419] ⑥ The lactic acid bacteria and heterolactic acid fermentation bacteria liquid are respectively: 0.5kg.

[0420] ⑦ The seed solution of photoautotrophic or chemoautotrophic photosynthetic bacteria and heterotrophic purple nitrogen-fixing photosynthetic bacteria are: 0.5kg respectively.

[0421] ⑧ 16kg fungus nutrient solution: mix 1kg potassium dihydrogen phosphate solution, 1kg calcium chloride, 1kg urea and 5kg sugar, add the mixture and sterile water in a weight ratio of 1:2 and dissolve in sterile water for later use.

[0422] ⑵Production:

[0423] First, mix ①, ②, and ③ of the above-mentioned preparations, then add ④ of the above-mentioned preparations and mix well, and ferment on the slant for 5 days, turning over once in the morning and evening every day; after 5 days, take a sample and dissolve it in water. When the pH value of the solution reaches 9.0, add the fermented material and sterile water in a weight ratio of 1:3.5 and mix well. If the pH value does not reach 9.0, continue to ferment for 2 days; add ⑤ and ⑥ of the above-mentioned preparations and mix well, and then aerate and ferment for 10 days; after 10 days, when the pH value is tested to be 3, filter out the bacterial residue, take the bacterial liquid and add ⑧ of the above-mentioned preparations, mix well, and ferment statically for 2 days; then add ⑦ of the above-mentioned preparations and mix well, and then ferment statically for 3 days; after 3 days, package it in barrels to obtain the finished product.

[0424] A special fish fertilizer for aquaculture is used in the microbial treatment of saline-alkali water to restore it to various seafood for modern seawater farming.

[0425] The special fish fertilizer for aquaculture is prepared by mixing the following raw materials in the following weight ratios:

[0426] The seed powder of Clostridium thermocellum is: 0.1kg.

[0427] The seed powders of Actinomyces melanocyles, A.roseus and A.cellulose are 0.1kg respectively.

[0428] The seed powders of Aspergillus oryzae, Rhizopus, and Trichoderma viride are 0.1 kg respectively.

[0429] 18kg wheat bran mixture: Mix wheat bran and sterile water in a weight ratio of 1:1 and set aside.

[0430] The seed powder of Saccharomyces and Geotrichum candidum are 0.1kg respectively.

[0431] The lactic acid bacteria (LAB. Lactic acid bacteria) and heterolactic acid fermentation bacterial liquid are respectively: 0.5kg.

[0432] The seed solution of photoautotrophic or chemoautotrophic photosynthetic bacteria and heterotrophic purple nitrogen-fixing photosynthetic bacteria is 0.5kg respectively.

[0433] 16kg fungus nutrient solution: 1kg potassium dihydrogen phosphate solution, 1kg calcium chloride, 1kg urea, 5kg sugar, mix the mixture and sterile water in a weight ratio of 1:2, add sterile water to dissolve and set aside;

[0434] The preparation of the special fish fertilizer for aquaculture comprises the following steps:

[0435] ⑴Material preparation:

[0436] ① The seed powder of Thermocellulosic Clostridium is: 0.1kg.

[0437] ② The seed powder of black-red spiral actinomycetes, rose-colored actinomycetes, and fiber actinomycetes are: 0.1kg respectively.

[0438] ③ The seed powders of Aspergillus oryzae, Rhizopus, and Trichoderma viride are 0.1kg respectively.

[0439] ④18kg wheat bran mixture: Add wheat bran and sterile water in a weight ratio of 1:1 to sterile water and mix well for later use;

[0440] ⑤ The yeast and Geotrichum candidum seed powders are 0.1kg respectively.

[0441] ⑥ The lactic acid bacteria and heterolactic acid fermentation bacteria liquid are: 0.kg respectively.

[0442] ⑦ The seed solution of photoautotrophic or chemoautotrophic photosynthetic bacteria and heterotrophic purple nitrogen-fixing photosynthetic bacteria are: 0.5kg respectively.

[0443] ⑧ 16kg fungus nutrient solution: mix 1kg potassium dihydrogen phosphate solution, 1kg calcium chloride, 1kg urea and 5kg sugar, add the mixture and sterile water in a weight ratio of 1:2 and dissolve in sterile water for later use.

[0444] ⑵Production:

[0445] First, mix ①, ②, and ③ of the above-mentioned preparations, then add ④ of the above-mentioned preparations and mix well, and ferment on the slant for 5 days, turning over once in the morning and evening every day; after 5 days, take a sample and dissolve it in water. When the pH value of the solution reaches 9.0, add the fermented material and sterile water in a weight ratio of 1:3.5 and mix well. If the pH value does not reach 9.0, continue to ferment for 2 days; add ⑤ and ⑥ of the above-mentioned preparations and mix well, and then aerate and ferment for 10 days; after 10 days, when the pH value is tested to be 3, filter out the bacterial residue, take the bacterial liquid and add ⑧ of the above-mentioned preparations, mix well, and ferment statically for 2 days; then add ⑦ of the above-mentioned preparations and mix well, and then ferment statically for 3 days; after 3 days, package it in barrels to obtain the finished product.

[0446] Embodiment 2:

[0447] A method for reducing saline-alkali water to modern seawater suitable for aquaculture of various seafood by using microorganisms, comprising the following steps:

[0448] First, saline water pretreatment:

[0449] Pump saline water with a salinity of 30‰ (mass per thousand) into the breeding pond, and turn on the air compressor to fully mix the air and saline water to promote the precipitation of some substances for subsequent treatment. The pH value controlled in the saline water pretreatment process is: 7.5.

[0450] Second, microbial culture:

[0451] Select purple halobacterium colonies that survive in coastal high-salinity and alkaline environments and purple halobacterium colonies that survive in plateau salt lakes, respectively, and after multi-stage enrichment, select the dominant halobacterium colonies of each, and mix the two to form purple halobacterium species for use;

[0452] A mixture is obtained by mixing saline-alkali water from saline-alkali ground with a salinity of 20‰ (mass per thousand), saline-alkali water from a salt lake with a salinity of 200‰ (mass per thousand), and high-salinity alkaline soil containing some organic matter. The mixture is steamed at 210°C, sealed in a light-transmitting incubator, and the purple mixed halobacterium species selected in step 1 above are connected. The air in the incubator is extracted, and the mixture is cultured using sunlight. When the substrate shows purple color by visual observation, the mixture can be expanded for standby use. The pH value controlled during inoculation and culture is: 8.5.

[0453] Third, reduction reaction:

[0454] The mixed purple salt bacteria cultured and expanded in the second step are inoculated into the saline water of the culture pond in the first step, and the waterwheel equipment is turned on for stirring for two hours and then turned off.

[0455] Carry out reduction reaction in a static water environment. After 10 days, when the water body shows reddish brown or dark brown color, add heterotrophic photosynthetic bacteria liquid, autotrophic photosynthetic bacteria liquid and yeast powder. After 10 days of reaction, add special aquaculture fish fertilizer preparation, turn on the oxygenation equipment 24 hours a day, react for 3 days, and control the pH value from 7.5 to 8.8.

[0456] Fourth, water quality monitoring and adjustment:

[0457] Monitor various indicators of the reduced saline-alkali water in real time, adjust the inoculation amount and type of microorganisms within the range of the first to third steps mentioned above according to the biological characteristics of seafood life, and detect the reduction and conversion process of the saline-alkali water to ensure that the water quality meets the requirements of various seafood farming.

[0458] Fifth, farmed seafood:

[0459] The restored saline-alkali water is used for seafood farming experiments; first, sea fish fry, shrimp, crab fry, and sea shellfish fry are tried. When more than 90% of them can survive after 48 hours, it proves that the restoration of seawater is successful.

[0460] A method for reducing saline-alkali water to modern seawater suitable for aquaculture of various seafood by using microorganisms, comprising the following specific steps:

[0461] First, saline water pretreatment:

[0462] 6688 cubic meters of saline water with a salinity of 30‰ (mass per thousand) was pumped into the breeding pond, and the air compressor was turned on to fully mix the air and the saline water to promote the precipitation of some substances for subsequent treatment. The pH value controlled in the saline water pretreatment process was: 7.5.

[0463] Second, microbial culture:

[0464] Select purple halobacterium colonies that survive in coastal high-salinity and alkaline environments and purple halobacterium colonies that survive in plateau salt lakes respectively. After multi-stage enrichment, select the superior halobacterium colonies of each and mix the two into 510 grams of purple halobacterium species for later use.

[0465] A mixture is obtained by mixing 5010 g of saline-alkali water from saline-alkali ground with a salinity of 20‰ (mass per thousand), 5010 g of saline-alkali water from a salt lake with a salinity of 200‰ (mass per thousand), and 510 g of high-salinity alkaline soil containing some organic matter. The mixture is steamed at 210°C, placed in a light-transmitting incubator and sealed, and the purple mixed halobacterium species selected in the first step is connected to the incubator. The air in the incubator is extracted and cultured using sunlight. When the matrix shows purple color by visual observation, it can be expanded for use. The pH value controlled during inoculation and culture is: 8.5.

[0466] Third, reduction reaction:

[0467] The mixed purple halobacterium cultured and expanded in the second step is inoculated into the saline water of the breeding pond in the first step at a rate of 20 grams per cubic meter of saline water, and the waterwheel equipment is turned on for stirring for two hours and then turned off.

[0468] Carry out reduction reaction in a static water environment. After 10 days, when the water body appears reddish brown or brown by visual inspection, add 20 grams of heterotrophic photosynthetic bacteria solution with a concentration of 5 billion / ml per cubic meter of water, add 20 grams of autotrophic photosynthetic bacteria solution with a concentration of -5 billion / ml per cubic meter of water, and add 20 grams of yeast powder with a specification of 10 billion / gram per cubic meter of water. After 10 days of joint reaction, add 31 grams of special aquaculture fish fertilizer preparation per cubic meter of water. Turn on the oxygenation equipment 24 hours a day, react for 3 days, and control the pH from 7.5 to 8.8.

[0469] Fourth, water quality monitoring and adjustment:

[0470] Monitor various indicators of the reduced saline-alkali water in real time, adjust the inoculation amount and type of microorganisms within the range of the first to third steps mentioned above according to the biological characteristics of seafood life, and detect the reduction and conversion process of the saline-alkali water to ensure that the water quality meets the requirements of various seafood farming.

[0471] Fifth, farmed seafood:

[0472] The restored saline-alkali water is used for seafood farming experiments; first, sea fish fry, shrimp, crab fry, and sea shellfish fry are tried. When more than 90% of them can survive after 48 hours, it proves that the restoration of seawater is successful.

[0473] The special aquaculture fish fertilizer preparation is connected, the oxygenation equipment is turned on 24 hours a day, and after three days of reaction, beneficial mixed concentrated algae liquid and beneficial zooplankton species are connected.

[0474] The re-introduction of beneficial mixed concentrated algae liquid and beneficial zooplankton species respectively refers to re-introduction of 50 grams of beneficial mixed concentrated algae liquid including triangular brown finger algae, marine green algae, and coralline algae per cubic meter of water, and re-introduction of 50 grams of live beneficial zooplankton including copepods such as Daphnia pulex and Daphnia pulex per cubic meter of water.

[0475] The pH value of the fourth water quality monitoring and adjustment and the fifth seafood farming two steps is 8.8.

[0476] The various seafoods include: golden drum fish, golden pomfret, pearl grouper, eastern star grouper, yellowtail snapper (yellow-legged sea bream), large oyster, East China Sea pearl, sea mullet (blackhead fish), mud fish, and seaweed.

[0477] The various seafoods described above do not require any domestication process and can be directly put into cultivation.

[0478] The special fish fertilizer for aquaculture is prepared by mixing the following raw materials in the following weight ratios:

[0479] The seed powder of Clostridium thermocellum is: 0.2kg.

[0480] The seed powders of Actinomyces melanocyles, A.roseus and A.cellulose are 0.2kg respectively.

[0481] The seed powders of Aspergillus oryzae, Rhizopus and Trichoderma viride are 0.2 kg respectively.

[0482] 20kg wheat bran mixture: Mix wheat bran and sterile water in a weight ratio of 1:1 and set aside.

[0483] The seed powder of Saccharomyces and Geotrichum candidum are 0.2kg respectively.

[0484] The amount of lactic acid bacteria (LAB. Lactic acid bacteria) and heterolactic acid fermentation bacterial liquid were respectively: 0.6kg.

[0485] The seed solution of photoautotrophic or chemoautotrophic photosynthetic bacteria and heterotrophic purple nitrogen-fixing photosynthetic bacteria is 0.6 kg respectively.

[0486] 26kg fungus nutrient solution: 3kg potassium dihydrogen phosphate solution, 1.5kg calcium chloride, 1.5kg urea, and 7kg sugar are mixed, and the mixture is added to sterile water in a weight ratio of 1:2 and dissolved for later use.

[0487] The preparation of the special fish fertilizer for aquaculture comprises the following steps:

[0488] ⑴Material preparation:

[0489] ① The seed powder of Thermocellulosic Clostridium is: 0.2kg.

[0490] ② The seed powder of black-red spiral actinomycetes, rose-colored actinomycetes, and fiber actinomycetes are: 0.2kg respectively.

[0491] ③ The seed powders of Aspergillus oryzae, Rhizopus, and Trichoderma viride are 0.2kg respectively.

[0492] ④20kg of wheat bran mixture: Add wheat bran and sterile water in a weight ratio of 1:1 to sterile water and mix well for later use.

[0493] ⑤ The yeast and Geotrichum candidum seed powders are 0.2kg respectively.

[0494] ⑥ The lactic acid bacteria and heterolactic acid fermentation bacteria liquid are respectively: 0.6kg.

[0495] ⑦ The seed solution of photoautotrophic or chemoautotrophic photosynthetic bacteria and heterotrophic purple nitrogen-fixing photosynthetic bacteria is: 0.6kg respectively.

[0496] ⑧ 26kg fungus nutrient solution: 3kg potassium dihydrogen phosphate solution, 1.5kg calcium chloride, 1.5kg urea, and 7kg sugar are mixed, and the mixture is added to sterile water in a weight ratio of 1:2 and dissolved for later use.

[0497] ⑵Production:

[0498] First, mix ①, ②, and ③ of the above-mentioned preparations, then add ④ of the above-mentioned preparations and mix well, and ferment on the slant for 5 days, turning over once in the morning and evening every day; after 5 days, take a sample and dissolve it in water. When the pH value of the solution reaches 9.0, add the fermented material and sterile water in a weight ratio of 1:3.5 and mix well. If the pH value does not reach 9.0, continue to ferment for 3 days; add ⑤ and ⑥ of the above-mentioned preparations and mix well, and then aerate and ferment for 10 days; after 10 days, when the pH value is tested to be 4, filter out the bacterial residue, take the bacterial liquid and add ⑧ of the above-mentioned preparations, mix well, and ferment statically for 2 days; then add ⑦ of the above-mentioned preparations and mix well, and then ferment statically for 3 days; after 3 days, package it in barrels to obtain the finished product.

[0499] A special fish fertilizer for aquaculture is used in the microbial treatment of saline-alkali water to restore it to various seafood for modern seawater farming.

[0500] The special fish fertilizer for aquaculture is prepared by mixing the following raw materials in the following weight ratios:

[0501] The seed powder of Clostridium thermocellum is: 0.2kg.

[0502] The seed powders of Actinomyces melanocyles, A.roseus and A.cellulose are 0.2kg respectively.

[0503] The seed powders of Aspergillus oryzae, Rhizopus and Trichoderma viride are 0.2 kg respectively.

[0504] 20kg wheat bran mixture: Mix wheat bran and sterile water in a weight ratio of 1:1 and set aside.

[0505] The seed powder of Saccharomyces and Geotrichum candidum are 0.2kg respectively.

[0506] The amount of lactic acid bacteria (LAB. Lactic acid bacteria) and heterolactic acid fermentation bacterial liquid were respectively: 0.6kg.

[0507] The seed solution of photoautotrophic or chemoautotrophic photosynthetic bacteria and heterotrophic purple nitrogen-fixing photosynthetic bacteria is 0.6 kg respectively.

[0508] 26kg fungus nutrient solution: 3kg potassium dihydrogen phosphate solution, 1.5kg calcium chloride, 1.5kg urea, and 7kg sugar are mixed, and the mixture is added to sterile water in a weight ratio of 1:2 and dissolved for later use.

[0509] The preparation of the special fish fertilizer for aquaculture comprises the following steps:

[0510] (1) Preparation:

[0511] ① The seed powder of Thermocellulosic Clostridium is: 0.2kg.

[0512] ② The seed powder of black-red spiral actinomycetes, rose-colored actinomycetes, and fiber actinomycetes are: 0.2kg respectively.

[0513] ③ The seed powders of Aspergillus oryzae, Rhizopus, and Trichoderma viride are 0.2kg respectively.

[0514] ④20kg of wheat bran mixture: Add wheat bran and sterile water in a weight ratio of 1:1 to sterile water and mix well for later use.

[0515] ⑤ The yeast and Geotrichum candidum seed powders are 0.2kg respectively.

[0516] ⑥ The lactic acid bacteria and heterolactic acid fermentation bacteria liquid are respectively: 0.6kg.

[0517] ⑦ The seed solution of photoautotrophic or chemoautotrophic photosynthetic bacteria and heterotrophic purple nitrogen-fixing photosynthetic bacteria is: 0.6kg respectively.

[0518] ⑧ 26kg fungus nutrient solution: 3kg potassium dihydrogen phosphate solution, 1.5kg calcium chloride, 1.5kg urea, 7kg sugar. Mix the mixture and sterile water in a weight ratio of 1:2 and dissolve in sterile water for later use.

[0519] ⑵Production:

[0520] First, mix ①, ②, and ③ of the above-mentioned preparations, then add ④ of the above-mentioned preparations and mix well, and ferment on the slant for 5 days, turning over once in the morning and evening every day; after 5 days, take a sample and dissolve it in water. When the pH value of the solution reaches 9.0, add the fermented material and sterile water in a weight ratio of 1:3.5 and mix well. If the pH value does not reach 9.0, continue to ferment for 3 days; add ⑤ and ⑥ of the above-mentioned preparations and mix well, and then aerate and ferment for 10 days; after 10 days, when the pH value is tested to be 4, filter out the bacterial residue, take the bacterial liquid and add ⑧ of the above-mentioned preparations, mix well, and ferment statically for 2 days; then add ⑦ of the above-mentioned preparations and mix well, and then ferment statically for 3 days; after 3 days, package it in barrels to obtain the finished product.

[0521] The test data of the saline-alkali water after being treated by Example 1-2 are as follows:

[0522] 1) Xinjiang Kezhou Zhanyu Aquatic Products Company Breeding Base No. 1. (See attached Figure 6 )

[0523] Water quality test record on May 28

[0524] Salt water inlet (#1) Salinity: 11 parts per thousand

[0525] PH value:8.9

[0526] Nitrite concentration: 0.12

[0527] Calcium ion concentration: 200mg / L

[0528] Magnesium ion concentration: 61mg / L

[0529] Ammonia content: 0

[0530] Potassium ion content: 261mg / L

[0531] Total alkalinity content: 290mg / L

[0532] Bicarbonate alkalinity: 110mg / L.

[0533] Freshwater Inlet (#2)

[0534] Salinity: 0 parts per thousand

[0535] PH value: 7.5

[0536] Nitrite concentration: 0.05

[0537] Calcium ion concentration: 100mg / L

[0538] Magnesium ion concentration: 182mg / L

[0539] Ammonia content: 0

[0540] Potassium ion content: 131mg / L

[0541] Total alkalinity content: 210mg / L

[0542] Bicarbonate alkalinity: 190 mg / L.

[0543] Area A Datang (#3)

[0544] Salinity: 10 parts per thousand

[0545] PH value: 8.3

[0546] Nitrite concentration: 0.10

[0547] Calcium ion concentration: 100mg / L

[0548] Magnesium ion concentration: 300mg / L

[0549] Ammonia content: 0

[0550] Potassium ion content: 148mg / L

[0551] Total alkalinity content: 280mg / L

[0552] Bicarbonate alkalinity: 180mg / L.

[0553] Area B Datang (#4)

[0554] Salinity: 11 parts per thousand

[0555] PH value: 8.5

[0556] Nitrite concentration: 0.10

[0557] Calcium ion concentration: 100mg / L

[0558] Magnesium ion concentration: 300mg / L

[0559] Ammonia content: 0

[0560] Potassium ion content: 155mg / L

[0561] Total alkalinity content: 270mg / L

[0562] Bicarbonate alkalinity: 170mg / L.

[0563] Area C Datang (#5)

[0564] Salinity: 10.5 parts per thousand

[0565] PH value: 8.5

[0566] Nitrite concentration: 0.12

[0567] Calcium ion concentration: 100mg / L

[0568] Magnesium ion concentration: 337mg / L

[0569] Ammonia content: 0

[0570] Potassium ion content: 155mg / L

[0571] Total alkalinity content: 270mg / L

[0572] Bicarbonate alkalinity: 170mg / L.

[0573] 2) Xinjiang Kezhou Zhanyu Aquatic Products Company Breeding Base No. 1. (See attached Figure 7 )Water quality test record on May 31

[0574] Salt water inlet (#1) Salinity: 11 parts per thousand PH value: 8.5

[0575] Nitrite concentration: 0.11

[0576] Calcium ion concentration: 200mg / L

[0577] Magnesium ion concentration: 61mg / L

[0578] Ammonia content: 0

[0579] Potassium ion content: 261mg / L

[0580] Total alkalinity content: 290mg / L

[0581] Bicarbonate alkalinity: 110mg / L.

[0582] Freshwater Inlet (#2)

[0583] Salinity: 0 parts per thousand

[0584] PH value: 7.8

[0585] Nitrite concentration: 0.05

[0586] Calcium ion concentration: 100mg / L

[0587] Magnesium ion concentration: 182mg / L

[0588] Ammonia content: 0

[0589] Potassium ion content: 131mg / L

[0590] Total alkalinity content: 210mg / L

[0591] Bicarbonate alkalinity: 190 mg / L.

[0592] Area A Datang (#3)

[0593] Salinity: 10 parts per thousand

[0594] PH value: 8.3

[0595] Nitrite concentration: 0.05

[0596] Calcium ion concentration: 100mg / L

[0597] Magnesium ion concentration: 300mg / L

[0598] Ammonia content: 0

[0599] Potassium ion content: 148mg / L

[0600] Total alkalinity content: 280mg / L

[0601] Bicarbonate alkalinity: 180mg / L.

[0602] Area B Datang (#4)

[0603] Salinity: 11 parts per thousand

[0604] PH value: 8.5

[0605] Nitrite concentration: 0.05

[0606] Calcium ion concentration: 100mg / L

[0607] Magnesium ion concentration: 300mg / L

[0608] Ammonia content: 0

[0609] Potassium ion content: 155mg / L

[0610] Total alkalinity content: 270mg / L

[0611] Bicarbonate alkalinity: 170mg / L.

[0612] Area C Datang (#5)

[0613] Salinity: 10.5 parts per thousand

[0614] PH value: 8.3

[0615] Nitrite concentration: 0.10

[0616] Calcium ion concentration: 100mg / L

[0617] Magnesium ion concentration: 337mg / L

[0618] Ammonia content: 0

[0619] Potassium ion content: 155mg / L

[0620] Total alkalinity content: 270mg / L

[0621] Bicarbonate alkalinity: 170mg / L.

[0622] The water source after successful restoration in Example 1-2 is used to culture various seafood with obvious effect.

[0623] For example, in the breeding base of Zhanyu Company of Kezhou Kizilsu Kirgiz Autonomous Prefecture (referred to as Kezhou) of Shishixian Breeding Group, located in the vast Niuerkul Salt Lake, the breeding of seafood is very successful. The following are the achievements:

[0624] 1. The stocking time of golden drum fish is July 10, 2024, and the testing time is August 2. The body length is 11 cm and the weight is 100 grams; (See attached for details) Fig.12 )

[0625] 2. The stocking time of golden pomfret is June 13, 2024, and the testing time is August 2. The body length is 13 cm and the weight is 195 g; (See Fig.13 )

[0626] 3. The stocking time of Chinese pearl grouper is June 10, 2024, and the testing time is August 2. The body length is 20 cm and the weight is 400 g; (See attached for details) Fig.15 )

[0627] 4. The stocking time of the yellow-footed catfish (Yellow-footed catfish) is June 13, 2024, and the testing time is August 2. The body length is 15 cm and the weight is 160 grams; (See the attached Fig.11 )

[0628] 5. The oyster seeding time is July 1, 2024, and the testing time is August 2. The body length is 48.4 mm; (See attached for details Fig.17 )

[0629] 6. The Donghai pearl seedlings were released on June 1, 2024, and the test was conducted on August 2. The body length is 14.1 mm, and the pearl is 0.69; (See attached for details) Fig.16 )

[0630] 7. The stocking time of sea mullet (horsehead fish) is the 15th day of the first lunar month in 2024, and the testing time is August 2nd. The body length is 35 cm and the weight is 750 g; (See attached for details) Fig.14 )

[0631] 8. The stocking time of giant pearl grouper is May 1, 2024, and the testing time is August 2. The body length is 30 cm and the weight is 1003 grams; (See attached for details) Fig.18 )

[0632] 9. The stocking time of mudfish is June 25, 2024, and the testing time is August 2. The body length is 14 cm and the weight is 150 g; (See attached for details) Fig.19 )

[0633] 10. The seedling time of seaweed is July 1, 2024, and the testing time is August 2. The salinity is 22‰, the growth value is good, and the yield per mu is about 3 tons per month; (See attached for details Fig. 20 )

[0634] The material cost of the present invention is mainly: two bottles of the sister products of the aquaculture-specific fish fertilizer water management agent (type A or type B), aquatic biological health agent (type C), and water balance agent (type 1 or type 2) are put in, 500 grams per bottle, and the sales price of each 500 grams of the three products is 25 yuan. (See the submitted supporting documents: the product manual of the applicant for details)

[0635] Calculated based on 10,000 cubic meters of water: three products are added once a week for three consecutive weeks to complete the restoration of seawater. Two bottles (1000 grams) of one of the three products are added each week, and the cost is 50 yuan. Six bottles (3000 grams) of the three products are added in total, and the cost is 150 yuan. The cost of other materials including machinery, heterotrophic photosynthetic bacteria liquid, autotrophic photosynthetic bacteria liquid, yeast powder, etc. is 25 yuan, a total of 225 yuan, once a week × 3 weeks = 675 yuan.

[0636] Total cost: Only 675 yuan is required to restore 10,000 cubic meters of water.

[0637] The invention process of using microorganisms to restore Xinjiang's saline water into modern seawater:

[0638] The inventor noticed some shallow ponds in the salt lake, some of which were red and some were purple, and speculated that there were microorganisms in them. The inventor sampled several kilograms of red and purple saline water respectively, and analyzed these water samples, and found that these colored waters contained organic matter and microorganisms. Through microscopic observation, a kind of microorganism and fungus was indeed found. As long as there are organic matter in an area, there will be microorganisms. Southern Xinjiang was part of the Mediterranean hundreds of millions of years ago, and was isolated from the Mediterranean due to crustal movement. After hundreds of millions of years of evaporation, the fresh water vaporized and disappeared, leaving behind various salts in the sea water. The chemical and physical structure of these salts changes with the continuous evaporation of seawater, and the salinity continues to increase, which is not suitable for modern fish to survive. However, modern seawater changes every day, and the salinity and other organic matter are also constantly changing, which is suitable for modern fish to survive. The experiment initially carried out to reduce salinity cultivation, but the effect was not good at first. The inventor collected water samples next to the salt lake. Recalling the origin of ancient earth organisms that I have learned in the past period, and combined with the information I have consulted, the inventor found three problems:

[0639] First, the inventor discovered that the first organisms to appear on Earth were the archaea Halobacterium. The core chemical components of photosynthesis by Halobacterium and other archaea are retinal and various microorganisms that can use green light. At that time, there was neither oxygen nor organic matter on Earth, but they were still able to survive, and their vitality was extremely tenacious. Later, Halobacterium and various microorganisms that could use green light gradually converted inorganic matter into organic matter. The ancient primitive Earth was anaerobic, and today the Halobacterium on Earth can survive in salt lakes, which is the result of continuous evolution to adapt to the oxygen environment. In this regard, the Halobacterium in the salt lake must be different from their ancestors.

[0640] Secondly, the reddish purple color of Halobacteria is because they can utilize the green light with the most energy in sunlight and can survive in extreme environments. This shows that they are the earliest organisms on Earth to utilize light energy. The Halobacteria that dominated the primitive ocean mainly absorbed green light, but not red and violet light. Later photosynthetic organisms could not utilize green light, so they could only evolve to absorb red and violet light that Halobacteria did not absorb, that is, chlorophyll microorganisms (cyanobacteria and blue-green algae).

[0641] Third, a series of changes that occurred in the ancient Earth were attributed to the emergence of cyanobacteria and blue algae. They continued to multiply and converted inorganic matter and sunlight into organic matter. Organic matter in the Earth's oceans continued to accumulate, and then heterotrophic and autotrophic photosynthetic bacteria appeared on Earth. These microorganisms that can survive in anaerobic and aerobic environments appeared on Earth, and they all have common biological characteristics, that is, they all rely on sunlight and inorganic and organic matter to participate in life activities.

[0642] The life activities of the above-mentioned microorganisms caused some subtle changes in the ancient earth. Cyanobacteria were difficult to grow on the surface of the ancient ocean at first, because ancient cyanobacteria relied on red and purple light and were afraid of green light. Over time, ancient cyanobacteria gradually adapted to the environment of the ocean surface from the bottom of the ocean. However, halobacteria need to grow on the surface of the ancient ocean, because halobacteria mainly absorb green light to carry out life activities. Therefore, the water on the entire earth was purple at that time. Cyanobacteria constantly changed themselves, adapted to the upper environment of the ancient ocean and multiplied in large numbers, releasing a large amount of oxygen in their life activities. Due to the accumulation of oxygen in the earth's environment, the ancient halobacteria gradually died. Cyanobacteria also migrated from the seabed to the middle and upper parts of the sea. This life evolution of cyanobacteria has gained the opportunity to reproduce in large numbers. The large-scale reproduction of cyanobacteria continuously releases oxygen, and the halobacteria slowly die.

[0643] The remains of the dead salt bacteria were just decomposed by heterotrophic and autotrophic photosynthetic bacteria. These two types of photosynthetic bacteria converted the inorganic matter that was not suitable for the growth and life of marine life on the ancient earth into organic matter; and then converted the organic matter that was not suitable for the growth of seafood into inorganic matter that was suitable for the growth and life of marine life and many seafood, providing a large amount of nutrients for cyanobacteria. Cyanobacteria continuously absorb these nutrients and reproduce in large numbers on the earth. Their life activities also continuously supply oxygen to the earth, creating today's earth life.

[0644] Inspired by the above three points, the inventor mixed saline-alkali soil, underground saline-alkali water and salt lake water into three different specific gravities, one of which was adjusted to 15‰, the second to 20‰, and the third to 30‰. Then high-temperature inactivation treatment was carried out. At that time, the conditions were limited and the water could only be heated to 200 degrees in an electric rice cooker. After high-temperature inactivation, after testing, it was found that there were basically no organisms in the water. Then the inactivated water samples were placed in three containers, and the three water samples were placed in a transparent container, and the salt bacteria species enriched from the salt lake were placed in it. The air was extracted and then placed in the sun. After about 10 days, the color of the original inoculation changed from slightly reddish and slightly purple to pink. After another 5 days, the color changed from pink to red. This color change is a characteristic of the transformation of the microorganisms inside from juveniles to adults (this is also the knowledge within the scope of the inventor's cognition). The inventor went to other salt lakes for investigation and returned five days later. After about 20 days, it was observed that its color became the same as that of the salt lake. After testing and testing, it was found that the microorganisms in the container had matured. In the inventor's understanding, as long as there are organisms in the salt lake, inorganic matter can be assimilated into organic matter. It should be known that organic matter contains many components. All bacteria or algae contain sugars, amino acids, calcium, magnesium, phosphorus, potassium and other components. After continuous accumulation, it is endless. According to this theoretical analysis, it is in line with the scientific theory of material transformation. After the test, the substances in the sample water have reached the conversion data required by the inventor.

[0645] Then, the inventor brought the test water back to Qinzhou City. The inventor's test equipment in Qinzhou City is relatively complete. The test found that the data obtained by the conversion of the above saline-alkali water by salt bacteria was 87% similar to the current seawater. At that time, the inventor was very excited and immediately called a friend to explain the progress of this achievement. Then he asked his friend to send another 1,000 kilograms of water to Qinzhou City, and mixed the salt lake water, underground saline-alkali water, and water from the drainage ditch for cotton planting into 1,000 kilograms. After receiving the water samples, the inventor first tested the water. The conversion data that the earth could achieve in the past, the inventor firmly believed that he could also achieve it. In the past, the earth was transformed naturally, which required the continuous evolution and mutation of microbial species, and it took a long time. Now the inventor has greatly shortened the process of restoring ancient sea materials with the help of the biological characteristics of microorganisms in nature. The natural marine material reduction and transformation process of the ancient earth must not be as efficient as modern artificial transformation, because the inventor artificially accessed salt bacteria, cyanobacteria and photosynthetic bacteria, as well as a variety of beneficial bacteria. The transformation process of the ancient Earth's oceans required hundreds of millions of years, but now, through the above-mentioned methods, the transformation process of various salt substances left over from the ancient oceans can be completed in just 20-30 days.

[0646] The specific steps of reducing and converting saline-alkali water are as follows:

[0647] First of all, salt bacteria have the ability to convert salt into various substances such as ammonia, nitrite, and nitrate. Salt bacteria belong to the category of bacteria that can survive in high-salt environments. They can convert inorganic salts and organic substances in salt lakes into the nutrients they need through metabolism, and release some metabolites. Salt bacteria can obtain energy by converting organic or inorganic substances through their own characteristic functions, and at the same time realize the conversion of salt into other substances. These substances can maintain the osmotic pressure of cells in high-salinity environments, thereby protecting cells from damage caused by high-salinity environments. The substances discharged by salt bacteria during their life activities mainly include carbon dioxide, water, ammonia, acetic acid, etc. Among them, carbon dioxide is the product of cellular respiration, water is the product of metabolic reactions, ammonia is the product of protein metabolism, and acetic acid is the product of metabolism of certain organic acids. And these excreta will change with environmental changes. For example, under different salinity, temperature and pH conditions, the metabolic products of salt bacteria may be different. The bacteria of salt bacteria themselves contain a variety of substances such as proteins, nucleic acids, polysaccharides, and lipids. These substances are the basic components of cells and are of great significance to maintaining the structure and function of cells. The bacterial composition of Halobacterium is also affected by environmental factors. For example, in a high-salt environment, Halobacterium may synthesize some special proteins and polysaccharides to adapt to this environment. The patent of this invention uses this characteristic of Halobacterium to carry out the preliminary work of reducing and converting saline water, creating an excellent water living environment and material environment for the subsequent access of heterotrophic photosynthetic bacteria, autotrophic photosynthetic bacteria, and yeast.

[0648] Secondly, the environment and materials formed by the saline water after the initial reduction and transformation by the above-mentioned salt bacteria are not suitable for the survival and growth of current marine animals because they belong to the ancient material structure. Therefore, the saline water in the pool must be connected to heterotrophic photosynthetic bacteria. Heterotrophic photosynthetic bacteria have light as energy and various organic matter as the main carbon source. They can assimilate various organic matter to form their own substances and absorb and assimilate a large number of fine molecular organic matter in the water body. Prevent other harmful bacteria from converting organic matter into harmful sub-salt forms, which plays a role in treating sewage and purifying the environment; there are also autotrophic photosynthetic bacteria, which have the function of absorbing inorganic matter as their own nutrition, converting and utilizing ammonia nitrogen, nitrite, and hydrogen sulfide in the water body, effectively improving water quality, balancing pH value, and accelerating the circulation of nutrients in the water body; and yeast, which has the function of utilizing nutrients in the fermentation substrate to synthesize its own protein and B vitamins, and converting various organic sewage into bacterial proteins, vitamins, various enzymes, and hormones.

[0649] The above-mentioned fungi can transform and utilize the transformation substances of the salt bacteria and their own metabolites, secretions and other organic and inorganic substances and synthesize their own bacterial substances. This process belongs to the primary circulation production process of material transformation from nothing to something. This creates a good water environment and material environment for the bacterial flora of the patented product: aquaculture special fish fertilizer (ZL200910137979.6).

[0650] Finally, the patented product: the preparation flora of fish fertilizer for aquaculture (ZL200910137979.6) is connected. The active ingredients of this preparation flora are:

[0651] Thermocellus Bacillus, Latin name: Clostridium thermocellum;

[0652] Description: This fungus is suitable for high temperature fermentation and produces polycellulose enzyme complex in the fermented material, which can dissolve various fibers and lignin, and its solution contains a small amount of ethanol.

[0653] Actinomyces melanocyles, rose-colored actinomycetes, A. roseus, and cellulose actinomycetes, A. cellulose;

[0654] Description: These three types of bacteria have basically the same functions and properties. They can decompose organic matter and produce extracellular enzymes to decompose protein, cellulose, lignin, and chitin. Therefore, this property can be used to deal with substances with chitin cell walls. The chitinase secreted by this bacterial group can dissolve a variety of pathogenic hyphae.

[0655] Aspergillus oryzae, its Latin name: Aspergillus oryzae;

[0656] Description: This fungus can produce saccharifying mold, fiber mold and phytic acid mold in the fermented materials, and can degrade straight-chain and branched chain flour, crude fiber and phytic acid into various fine molecules and free substances, and degrade large molecular proteins into peptones, polypeptides and various amino acids.

[0657] Rhizopus, its Latin name: Rhizopus;

[0658] Description: This fungus is a fungus that helps mold fermentation. The secretions it produces can help catalyze decomposition during mold fermentation.

[0659] Trichoderma viride:

[0660] Description: This fungus is a kind of glucan hydrolyzing mold (enzyme). It has strong catalytic effect on the hydrolysis of cellulose and lignin.

[0661] Yeast, its Latin name: Saccharomyces, white fungus, its Latin name:

[0662] Geotrichumcandidum;

[0663] Description: These two types of bacteria have basically the same functions and properties. They can utilize nutrients in the fermentation substrate to synthesize their own proteins and B vitamins, and convert various organic wastewaters into bacterial proteins, vitamins, various enzymes, hormones, etc.

[0664] Lactic acid bacteria, its Latin name: LAB.Lactic acid bacteria;

[0665] Description: This bacterium can decompose carbohydrates in materials to form lactic acid, playing a balancing role in multi-bacteria fermentation.

[0666] Heterolactic fermentation bacteria:

[0667] Description: This bacterium can secrete acetic acid, succinic acid, hydrogen and carbon dioxide into the material during multi-bacteria fermentation, provide nutrition for other bacteria, and play a coordinating role in purifying the environment.

[0668] Photoautotrophic or chemoautotrophic photosynthetic bacteria,

[0669] Description: This bacterium absorbs inorganic substances as its own nutrition, transforms and utilizes ammonia nitrogen, nitrite, and hydrogen sulfide in the water, effectively improves water quality, balances pH value, and accelerates the circulation of nutrients in the water.

[0670] Heterotrophic purple nitrogen-fixing photosynthetic bacteria,

[0671] Description: This bacterium uses light as energy and various organic matter as the main carbon source. It can assimilate various organic matter to form its own substances. It can absorb and assimilate a large number of fine molecular organic matter in the water body. It prevents other harmful bacteria from converting organic matter into harmful subspecies, which is of great significance in treating sewage and purifying the environment.

[0672] The bacterial community has the functions of effectively inhibiting the reproduction of pathogenic bacteria of aquatic animals, solving the problem of aquaculture pollution, enhancing the disease resistance of animals and maintaining the ecological balance of water bodies. In aquaculture, there is no need to add fertilizers and chemicals to the pond. Just add a small amount of river water or seawater to the aquaculture pond regularly, and then apply the microbial preparation to obtain the amount of nutrients required by the microorganisms in the aquaculture pond, cultivate and reproduce abundant primary live bait and microbial species, form a tight cycle ecological environment and food chain in the water body, and quickly degrade organic matter and harmful substances. Through the large-scale reproduction and metabolites of microorganisms in the product, the organic matter in the water body is quickly and effectively degraded, and ammonia nitrogen, nitrite, sulfide, phosphorus and other harmful substances are decomposed. The bacterial community can reduce the substances converted from saline water by the above-mentioned halobacteria, heterotrophic photosynthetic bacteria, autotrophic photosynthetic bacteria, and yeast, so that they can adapt to the living environment that is beneficial to phytoplankton and zooplankton. The restored saline-alkali water body brings calcium, magnesium, potassium, trace elements and various organic substances to the water body through the massive reproduction and mutual survival of beneficial phytoplankton and beneficial zooplankton, and their life activities continue to accumulate in a cycle. The structure of the substances accumulated in the water body also changes continuously, and finally reaches the water quality standard for breeding marine fish, shrimps, crabs and shellfish.

[0673] The above-mentioned embodiments only express several implementation methods of the present invention, and the description thereof is relatively specific and detailed, but it cannot be understood as limiting the scope of the patent of the present invention. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the concept of the present invention, which all belong to the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention shall be subject to the attached claims.

Claims

1. A method for reducing saline-alkali water to modern seawater suitable for breeding various seafood by using microorganisms, characterized in that: The steps include: First, saline water pretreatment: Pump saline water with a salinity of 15‰-30‰ (mass per thousand) into the aquaculture pond, and turn on the air compressor to fully mix the air with the saline water to promote the precipitation of some substances for subsequent treatment. The pH value controlled in the saline water pretreatment process is: 7.3-7.5； Second, microbial culture: Select purple halobacterium colonies that survive in coastal high-salinity and alkaline environments and purple halobacterium colonies that survive in plateau salt lakes, respectively, and after multi-stage enrichment, select the dominant halobacterium colonies of each, and mix the two to form purple halobacterium species for use; A mixture is obtained by mixing saline-alkali water from saline-alkali underground with a salinity of 10-20‰ (mass per thousand), saline-alkali water from a salt lake with a salinity of 180-200‰ (mass per thousand), and high-salinity alkaline soil containing some organic matter. The mixture is steamed at a temperature of 200-210°C, and then sealed in a light-transmitting incubator. The purple mixed halobacterium species selected in step 1 above are introduced, and the air in the incubator is extracted. The culture is cultured using sunlight. When the substrate is visually observed to be purple, it can be expanded for standby use. The pH value controlled during inoculation and culture is: 8.3-8.5； Third, reduction reaction: The mixed purple halobacterium cultured and expanded in the second step is inoculated into the saline water of the culture pond in the first step, and the waterwheel equipment is turned on for stirring for two hours and then turned off; Carry out reduction reaction in a static water environment. After 5-10 days, when the water body shows reddish brown or brown by visual inspection, add heterotrophic photosynthetic bacteria liquid, autotrophic photosynthetic bacteria liquid, and yeast powder. After 5-10 days of reaction, add special aquaculture fish fertilizer preparation, open the oxygenation equipment 24 hours a day, react for 3 days, and control the pH value from 7.3-7.5 to 8.5-8.8; Fourth, water quality monitoring and adjustment: Monitor various indicators of the reduced saline-alkali water in real time, adjust the inoculation amount and type of microorganisms within the range of the first to third steps according to the biological characteristics of seafood life, and detect the reduction and conversion process of the saline-alkali water to ensure that the water quality meets the requirements of various seafood farming; Fifth, farmed seafood: The restored saline-alkali water is used for seafood farming experiments; first, sea fish fry, shrimp, crab fry, and sea shellfish fry are tried. When more than 90% of them can survive after 48 hours, it proves that the restoration of seawater is successful.

2. The method of claim 1, wherein the method utilizes microorganisms to reduce saline-alkali water to modern seawater suitable for breeding various seafood, characterized in that: The specific steps include: First, saline water pretreatment: Pump 6677-6688 cubic meters of saline water with a salinity of 15‰-30‰ (mass per thousand) into the breeding pond, and turn on the air compressor to fully mix the air with the saline water to promote the precipitation of some substances for subsequent treatment. The pH value controlled in the saline water pretreatment process is: 7.3-7.5； Second, microbial culture: Select purple halobacterium colonies that survive in coastal high-salinity and alkaline environments and purple halobacterium colonies that survive in plateau salt lakes, select the dominant halobacterium colonies from each after multi-stage enrichment, and mix the two to form 500-510 grams of purple halobacterium species for later use; 5000-5010 g of saline-alkali water from saline-alkali ground with a salinity of 10-20‰ (mass per thousand), 5000-5010 g of saline-alkali water from a salt lake with a salinity of 180-200‰ (mass per thousand), and 500-510 g of high-salinity alkaline soil containing some organic matter are mixed to obtain a mixture. After the mixture is steamed at 200-210°C, it is placed in a light-transmitting incubator and sealed, and the purple mixed halobacterium species selected in the first step is introduced, the air in the incubator is extracted, and the culture is carried out using sunlight. When the substrate is visually observed to be purple, it can be expanded for standby use. The pH value controlled during inoculation and culture is: 8.3-8.5； Third, reduction reaction: The mixed purple halobacterium cultured and expanded in the second step is inoculated into the saline water of the breeding pond in the first step at a rate of 10-20 grams per cubic meter of saline water, and the waterwheel equipment is turned on for stirring for two hours and then turned off; Carry out reduction reaction in a static water environment. After 5-10 days, when the water body shows reddish brown or brown by visual inspection, add 10-20 g of heterotrophic photosynthetic bacteria solution with a concentration of 3-5 billion / ml per cubic meter of water, add 10-20 g of autotrophic photosynthetic bacteria solution with a concentration of 3-5 billion / ml per cubic meter of water, and add 10-20 g of yeast powder with a specification of 10 billion / gram per cubic meter of water. After 5-10 days of joint reaction, add 30-31 g of special aquaculture fish fertilizer preparation per cubic meter of water, turn on the oxygenation equipment 24 hours a day, react for 3 days, and control the pH from 7.3-7.5 to 8.5-8.8; Fourth, water quality monitoring and adjustment: Monitor various indicators of the reduced saline-alkali water in real time, adjust the inoculation amount and type of microorganisms within the range of the first to third steps according to the biological characteristics of seafood life, and detect the reduction and conversion process of the saline-alkali water to ensure that the water quality meets the requirements of various seafood farming; Fifth, farmed seafood: The restored saline-alkali water is used for seafood farming experiments; first, sea fish fry, shrimp, crab fry, and sea shellfish fry are tried. When more than 90% of them can survive after 48 hours, it proves that the restoration of seawater is successful.

3. A method for reducing saline-alkali water to modern seawater suitable for breeding various seafood by using microorganisms according to claim 1 or 2, characterized in that: the special aquaculture fish fertilizer preparation is connected, the oxygenation equipment is turned on 24 hours a day, and after three days of reaction, beneficial mixed concentrated algae liquid and beneficial zooplankton species are connected.

4. According to claim 3, a method for reducing saline-alkali water to modern seawater suitable for breeding various seafood by using microorganisms, characterized in that the re-introduction of beneficial mixed concentrated algae liquid and beneficial zooplankton species refers to re-introducing 50 grams of beneficial mixed concentrated algae liquid including triangular brown finger algae, marine green algae, and coralline algae per cubic meter of water, and re-introducing 50 grams of beneficial zooplankton living bodies including copepods such as Daphnia pulex and Daphnia pulex per cubic meter of water.

5. A method for reducing saline-alkali water to modern seawater suitable for breeding various seafood by using microorganisms according to claim 1 or 2, characterized in that: The pH value of the fourth water quality monitoring and adjustment and the fifth seafood farming two steps is 8.5-8.

8.

6. A method for reducing saline-alkali water to modern seawater suitable for breeding various seafood by using microorganisms according to claim 1 or 2, characterized in that: The various seafoods include: golden drum fish, golden pomfret, pearl grouper, eastern star grouper, yellowtail snapper (yellow-legged sea bream), large oyster, East China Sea pearl, sea mullet (blackhead fish), mud fish, and seaweed.

7. A method for reducing saline-alkali water to modern seawater suitable for breeding various seafood by using microorganisms according to claim 1 or 2, characterized in that: The various seafoods described above do not require any domestication process and can be directly put into cultivation.

8. A method for reducing saline-alkali water to modern seawater suitable for breeding various seafood by using microorganisms according to claim 1 or 2, characterized in that: The special fish fertilizer for aquaculture is prepared by mixing the following raw materials in the following weight ratios: The seed powder of Clostridium thermocellum is 0.1 to 0.2 parts; The seed powders of Actinomyces melanocyles, A. roseus and A. cellulose are 0.1 to 0.2 parts respectively; The seed powders of Aspergillus oryzae, Rhizopus, and Trichoderma viride are 0.1 to 0.2 parts respectively; 18-20 parts of wheat bran mixture: Mix wheat bran and sterile water in a weight ratio of 1:1 and set aside; Saccharomyces and Geotrichum candidum seed powders are 0.1 to 0.2 parts respectively; Lactic acid bacteria (LAB. Lactic acid bacteria) and heterolactic acid fermentation bacteria liquid are 0.5-0.6 parts respectively; The seed solution of photoautotrophic or chemoautotrophic photosynthetic bacteria and heterotrophic purple nitrogen-fixing photosynthetic bacteria are 0.5 to 0.6 parts respectively; Mix 16-26 parts of fungus nutrient solution: 1-3 parts of potassium dihydrogen phosphate solution, 1-1.5 parts of calcium chloride, 1-1.5 parts of urea, and 5-7 parts of sugars, and add the mixture and sterile water in a weight ratio of 1:2 to dissolve the mixture in sterile water for later use.

9. A method for reducing saline-alkali water to modern seawater suitable for breeding various seafood by using microorganisms according to claim 1 or 2, characterized in that: The preparation of the special fish fertilizer for aquaculture comprises the following steps (parts involved are by weight): ⑴Material preparation: ① The seed powder of Thermocellulosic Clostridium is 0.1-0.2 parts; ② The seed powders of black-red spiral actinomycetes, rose-colored actinomycetes, and fiber actinomycetes are 0.1-0.2 parts respectively; ③ The seed powders of Aspergillus oryzae, Rhizopus, and Trichoderma viride are 0.1 to 0.2 parts respectively; ④18-20 parts of wheat bran mixture: wheat bran and sterile water are added to sterile water in a weight ratio of 1:1, mixed well and set aside; ⑤ Yeast and Geotrichum candidum seed powder are 0.1-0.2 parts respectively; ⑥ Lactic acid bacteria and heterolactic acid fermentation bacteria liquid are 0.5-0.6 parts respectively; ⑦ The seed solution of photoautotrophic or chemoautotrophic photosynthetic bacteria and heterotrophic purple nitrogen-fixing photosynthetic bacteria are 0.5-0.6 parts respectively; ⑧ 16-26 parts of fungus nutrient solution: 1-3 parts of potassium dihydrogen phosphate solution, 1-1.5 parts of calcium chloride, 1-1.5 parts of urea, and 5-7 parts of sugars are mixed, and the mixture is added to sterile water in a weight ratio of 1:2 to dissolve for later use; ⑵Production: First, mix ①, ②, and ③ of the above-mentioned preparations, then add ④ of the above-mentioned preparations and mix well, and ferment on the slant for 5 days, turning over once in the morning and evening every day; after 5 days, take a sample and dissolve it in water. When the pH value of the solution reaches 9.0, add the fermented material and sterile water in a weight ratio of 1:3.5 and mix well. If the pH value does not reach 9.0, continue to ferment for 2 to 3 days; add ⑤ and ⑥ of the above-mentioned preparations and mix well, and then aerate and ferment for 10 days; after 10 days, when the pH value is tested to be 3 to 4, filter out the bacterial residue, take the bacterial liquid and add ⑧ of the above-mentioned preparations, mix well, and ferment statically for 2 days; then add ⑦ of the above-mentioned preparations and mix well, and then ferment statically for 3 days; after 3 days, package it in barrels to obtain the finished product.

10. Application of a special fish fertilizer for aquaculture in microbial treatment of saline-alkali water to modern seawater aquaculture of various seafoods, characterized by: The special fish fertilizer for aquaculture is prepared by mixing the following raw materials in the following weight ratios: The seed powder of Clostridium thermocellum is 0.1 to 0.2 parts; The seed powders of Actinomyces melanocyles, A. roseus and A. cellulose are 0.1 to 0.2 parts respectively; The seed powders of Aspergillus oryzae, Rhizopus, and Trichoderma viride are 0.1 to 0.2 parts respectively; 18-20 parts of wheat bran mixture: Mix wheat bran and sterile water in a weight ratio of 1:1 and set aside; Saccharomyces and Geotrichum candidum seed powders are 0.1 to 0.2 parts respectively; Lactic acid bacteria (LAB. Lactic acid bacteria) and heterolactic acid fermentation bacteria liquid are 0.5-0.6 parts respectively; The seed solution of photoautotrophic or chemoautotrophic photosynthetic bacteria and heterotrophic purple nitrogen-fixing photosynthetic bacteria are 0.5 to 0.6 parts respectively; 16-26 parts of fungus nutrient solution: 1-3 parts of potassium dihydrogen phosphate solution, 1-1.5 parts of calcium chloride, 1-1.5 parts of urea, and 5-7 parts of sugars are mixed, and the mixture is added to sterile water in a weight ratio of 1:2 to dissolve for later use; The preparation of the special fish fertilizer for aquaculture comprises the following steps: ⑴Material preparation: ① The seed powder of Thermocellulosic Clostridium is 0.1-0.2 parts; ② The seed powders of black-red spiral actinomycetes, rose-colored actinomycetes, and fiber actinomycetes are 0.1-0.2 parts respectively; ③ The seed powders of Aspergillus oryzae, Rhizopus, and Trichoderma viride are 0.1 to 0.2 parts respectively; ④18-20 parts of wheat bran mixture: wheat bran and sterile water are added to sterile water in a weight ratio of 1:1, mixed well and set aside; ⑤ Yeast and Geotrichum candidum seed powder are 0.1-0.2 parts respectively; ⑥ Lactic acid bacteria and heterolactic acid fermentation bacteria liquid are 0.5-0.6 parts respectively; ⑦ The seed solution of photoautotrophic or chemoautotrophic photosynthetic bacteria and heterotrophic purple nitrogen-fixing photosynthetic bacteria are 0.5-0.6 parts respectively; ⑧ 16-26 parts of fungus nutrient solution: 1-3 parts of potassium dihydrogen phosphate solution, 1-1.5 parts of calcium chloride, 1-1.5 parts of urea, and 5-7 parts of sugars are mixed, and the mixture is added to sterile water in a weight ratio of 1:2 to dissolve for later use; ⑵Production: First, mix ①, ②, and ③ of the above-mentioned preparations, then add ④ of the above-mentioned preparations and mix well, and ferment on the slant for 5 days, turning over once in the morning and evening every day; after 5 days, take a sample and dissolve it in water. When the pH value of the solution reaches 9.0, add the fermented material and sterile water in a weight ratio of 1:3.5 and mix well. If the pH value does not reach 9.0, continue to ferment for 2 to 3 days; add ⑤ and ⑥ of the above-mentioned preparations and mix well, and then aerate and ferment for 10 days; after 10 days, when the pH value is tested to be 3 to 4, filter out the bacterial residue, take the bacterial liquid and add ⑧ of the above-mentioned preparations, mix well, and ferment statically for 2 days; then add ⑦ of the above-mentioned preparations and mix well, and then ferment statically for 3 days; after 3 days, package it in barrels to obtain the finished product.

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