Serum albumin function evaluation method, device, kit, equipment and medium

Abstract

The invention provides a serum albumin function evaluation method and device, a kit, equipment and a medium, the function evaluation method comprises the following steps: mixing a spin probe and a to-be-detected sample to obtain a to-be-detected mixture, the spin probe being a detection molecule having a free radical of unpaired electrons, the detection molecule is used for being combined with a target site of serum albumin in a to-be-detected sample; detecting the to-be-detected mixture based on an electron paramagnetic resonance technology to obtain a first detection map; the first detection spectrogram is compared with a preset spectrogram feature, detection information conforming to the preset spectrogram feature is obtained, and the detection information represents the proportion of the spin probe in the binding state to the spin probe in the free state; and generating a function evaluation result of the serum albumin in the to-be-detected sample according to the detection information. The function evaluation method can effectively evaluate the function of serum albumin.

Description

Technical Field

[0001] The present application relates to the field of biotechnology, and in particular to a method, device, kit, equipment and medium for evaluating the function of serum albumin. Background Art

[0002] Serum albumin is the most important protein in human plasma. Serum albumin has many important physiological functions, such as: (1) Maintaining plasma colloidal osmotic pressure, preventing water from seeping from blood vessels into tissue spaces, thereby maintaining blood volume. (2) Serum albumin has an immunomodulatory effect, which can inhibit inflammatory responses, reduce oxidative stress, and enhance the body's resistance to bacteria and viruses. (3) Serum albumin can stabilize endothelial cells, prevent endothelial cell damage and increased permeability, thereby reducing the occurrence of complications such as ascites and edema. (4) The free cysteine ​​in serum albumin can play an antioxidant role, remove reactive oxygen and nitric oxide in the body, and prevent cell damage.

[0003] In the related art, the concentration of serum albumin can be accurately detected, but the quality of serum albumin cannot be effectively detected, resulting in an inaccurate assessment of serum albumin. Summary of the invention

[0004] The present application provides a method, device, kit, equipment and medium for evaluating the function of serum albumin, which can accurately evaluate the function of serum albumin.

[0005] In a first aspect, the present application provides a method for evaluating the function of serum albumin, the method comprising:

[0006] Mixing a spin probe with a sample to be tested to obtain a test mixture, wherein the spin probe is a detection molecule of a free radical having unpaired electrons, and the detection molecule is used to bind to a target site of serum albumin in the sample to be tested;

[0007] The mixture to be tested is detected based on the electron paramagnetic resonance technology to obtain a first detection spectrum;

[0008] The first detection spectrum is compared with the preset spectrum characteristics to obtain detection information that conforms to the preset spectrum characteristics. The detection information represents the ratio of the spin probe in the bound state to the spin probe in the free state. The functional evaluation result of serum albumin in the sample to be tested is generated according to the detection information.

[0009] In some embodiments, before comparing the first detected spectrum with the preset spectrum feature, the process includes:

[0010] Controlling the electron paramagnetic resonance spectrometer to detect multiple reference samples using the first working parameter to obtain second detection spectra corresponding to different reference samples;

[0011] Generate a preset spectrum feature about the first magnetic field region according to the ratio of the spin probe in the bound state to the spin probe in the free state in the reference sample and the second detection spectrum;

[0012] The first detection spectrum is compared with the preset spectrum characteristics to obtain detection information that meets the preset spectrum characteristics, including:

[0013] The first detection spectrum obtained based on the first working parameter is compared with the preset spectrum characteristics to obtain detection information that meets the preset spectrum characteristics and is located in the first magnetic field area.

[0014] In some embodiments, the preset spectrogram features include:

[0015] (1) It has a triplet peak, and the coupling splitting interval value is (13G~17G);

[0016] (2) The g factor of the signal center is (1.96~2.04).

[0017] In some embodiments, the first operating parameters are: the dimensionless factor of the central magnetic field is 1.96-2.04, the scanning width is 50-500G, the modulation amplitude is 0.01-20G, the time constant is 0.01-1s, the microwave power is 0.02-200mW, and the test temperature is less than or equal to 40°C.

[0018] In some embodiments, the spin probe is one of stearic acid and its diffractors, stearate esters and their diffractors.

[0019] In some embodiments, the radical with an unpaired electron in the spin probe is a nitroxide radical.

[0020] In some embodiments, the spin probe comprises any one of 5-nitroxy stearic acid, 16-nitroxy stearic acid, 5-nitroxy stearate, and 16-nitroxy stearate.

[0021] In some embodiments, the spin probe is mixed with a sample to be tested to obtain a test mixture, comprising:

[0022] The first solution is mixed with the sample to be tested to obtain a mixture to be tested, wherein the first solution includes a first solvent and a spin probe dissolved in the first solvent, and the first solvent is dimethyl sulfoxide or ethanol.

[0023] In some embodiments, in the test mixture, the molar ratio of the spin probe to the serum albumin in the test sample is (1:1) to (7:1).

[0024] In a second aspect, the present application also provides a serum albumin function evaluation device, comprising:

[0025] A mixing module, mixing the spin probe with the sample to be tested to obtain a mixture to be tested, wherein the spin probe is a detection molecule of a free radical with unpaired electrons, and the detection molecule is used to bind to a target site of serum albumin in the sample to be tested;

[0026] A first detection module is used to detect the mixture to be detected based on electron paramagnetic resonance technology to obtain a first detection spectrum;

[0027] A first comparison module, used for comparing the first detection spectrum with a preset spectrum feature to obtain detection information that meets the preset spectrum feature, wherein the detection information represents the ratio of the spin probe in a bound state to the spin probe in a free state;

[0028] The first analysis module is used to generate a functional evaluation result of serum albumin in the sample to be tested according to the detection information.

[0029] In a third aspect, the present application also provides a serum albumin function evaluation kit, comprising:

[0030] The first solution is used to mix with the sample to be tested. The first solution includes a first solvent and a spin probe dissolved in the first solvent. The spin probe is a detection molecule of a free radical with unpaired electrons. The detection molecule is used to bind to a target site of serum albumin in the sample to be tested.

[0031] In a fourth aspect, the present application also provides a serum albumin functional evaluation device, comprising a processor and a memory, wherein the memory stores programs or instructions that can be executed on the processor, and when the programs or instructions are executed by the processor, the steps of the serum albumin functional evaluation method provided in the first aspect are implemented.

[0032] In a fifth aspect, the present application further provides a readable storage medium, on which a program or instruction is stored, and when the program or instruction is executed by a processor, the steps of the method for functional evaluation of serum albumin as provided in the first aspect are implemented.

[0033] In a sixth aspect, the present application further provides a computer program product, which is stored in a storage medium and is executed by at least one processor to implement the steps of the method for functional evaluation of serum albumin as provided in the first aspect.

[0034] In the technical solution of the present application, a detection molecule with a free radical is used as a spin probe, mixed with a sample to be tested, and the binding site of serum albumin is used to bind to the detection molecule, so that serum albumin with normal function can bind to the spin probe; the first detection spectrum is compared with the preset spectrum characteristics to obtain detection information that conforms to the preset spectrum characteristics, and the detection information represents the ratio of the spin probe in the bound state to the spin probe in the free state, so that a functional evaluation result of serum albumin in the sample to be tested can be generated according to the detection information, and the evaluation result can represent the transport capacity and activity of serum albumin for the detection molecule. BRIEF DESCRIPTION OF THE DRAWINGS

[0035] The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate embodiments consistent with the present application and, together with the description, serve to explain the principles of the present application.

[0036] Figure 1 This is a schematic diagram of the process of the method for evaluating the function of serum albumin provided in one embodiment of the present application;

[0037] Figure 2 The EPR spectrum of 16-nitrogen oxide free radical stearic acid provided in an embodiment of the present application;

[0038] Figure 3 is an EPR spectrum of a spin probe in a bound state in one embodiment of the present application;

[0039] Figure 4 is an EPR spectrum of a spin probe in a free state in one embodiment of the present application;

[0040] Figure 5 The EPR spectra of the samples to be tested having different ratios of the spin probes in the bound state and the spin probes in the free state in one embodiment of the present application;

[0041] Figure 6 It is a schematic diagram of the structure of a serum albumin function evaluation device provided in one embodiment of the present application;

[0042] Figure 7 It is a schematic diagram of the hardware structure of a serum albumin function evaluation device provided in one embodiment of the present application.

[0043] The above drawings have shown clear embodiments of the present application, which will be described in more detail later. These drawings and text descriptions are not intended to limit the scope of the present application in any way, but to illustrate the concept of the present application to those skilled in the art by referring to specific embodiments. DETAILED DESCRIPTION

[0044] The various embodiments or implementation schemes in this specification are described in a progressive manner, and each embodiment focuses on the differences from other embodiments.

[0045] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "illustrative embodiments", "examples", "specific examples", or "some examples" means that the specific features, structures, materials, or characteristics described in conjunction with the implementation or examples are included in at least one implementation or example of the present application. In this specification, the schematic representation of the above terms does not necessarily refer to the same implementation or example. Moreover, the specific features, structures, materials, or characteristics described may be combined in any one or more implementations or examples in a suitable manner.

[0046] In addition, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include at least one of the features. In the description of this application, the meaning of "plurality" is at least two, such as two, three, etc., unless otherwise clearly and specifically defined.

[0047] Serum albumin is the most important protein in human plasma. Serum albumin has many important physiological functions, such as: (1) Maintaining plasma colloidal osmotic pressure, preventing water from seeping from blood vessels into tissue spaces, thereby maintaining blood volume. (2) Serum albumin has an immunomodulatory effect, which can inhibit inflammatory responses, reduce oxidative stress, and enhance the body's resistance to bacteria and viruses. (3) Serum albumin can stabilize endothelial cells, prevent endothelial cell damage and increased permeability, thereby reducing the occurrence of complications such as ascites and edema. (4) The free cysteine ​​in serum albumin can play an antioxidant role, remove reactive oxygen and nitric oxide in the body, and prevent cell damage.

[0048] In the related art, the concentration of serum albumin can be accurately detected, but the transport capacity and activity of serum albumin cannot be effectively detected, resulting in inaccurate evaluation of serum albumin.

[0049] In order to solve the above technical problems, the present application provides a method for evaluating the function of serum albumin, which can accurately evaluate the function of serum albumin.

[0050] Please refer to Figure 1 The method for evaluating the function of serum albumin provided in the present application comprises the following steps:

[0051] S100, mixing a spin probe with a sample to be tested to obtain a test mixture, wherein the spin probe is a detection molecule of a free radical having unpaired electrons, and the detection molecule is used to bind to a target site of serum albumin in the sample to be tested;

[0052] S200, detecting the mixture to be detected based on electron paramagnetic resonance technology to obtain a first detection spectrum;

[0053] S300, comparing the first detection spectrum with the preset spectrum characteristics to obtain detection information that meets the preset spectrum characteristics, the detection information characterizes the ratio of the spin probes in the bound state and the spin probes in the free state, and generating a functional evaluation result of serum albumin in the sample to be tested based on the detection information.

[0054] The sample to be tested used in S100 can be obtained by performing venipuncture on the user to be tested, collecting a blood sample, centrifuging the blood sample, and taking the upper serum. For example: perform venipuncture on the user to be tested, collect 1mL to 5ml of blood sample, centrifuge the blood sample at 4°C for 10min, and the centrifugal speed is 3000r / min. After centrifugation, extract the upper serum, and the extract is the sample to be tested. The sample to be tested can be stored at -80°C.

[0055] Serum albumin has multiple binding sites, and small molecules bind to serum albumin through the binding sites to provide serum albumin for transport. Serum albumin under physiological conditions and serum albumin under pathological conditions have different binding abilities, which makes the two have different transport abilities. For example: serum albumin has seven fatty acid binding sites. Under physiological conditions, serum albumin will transport fatty acids, but under pathological conditions, the ability of serum albumin to bind fatty acids decreases. The spin probe is mixed with the sample to be tested, and serum albumin under physiological conditions binds to the spin probe, so that the spin probe is in a bound state in the mixture. Under pathological conditions, serum albumin does not bind to the spin probe, or the ability to bind to the spin probe decreases, so that the spin probe is in a free state in the mixture.

[0056] In S200, an electron paramagnetic resonance spectrometer can be used to test the mixture based on electron paramagnetic resonance spectroscopy (EPR). Electron paramagnetic resonance technology can directly detect unpaired electrons, and has the advantages of high sensitivity and in-situ non-invasiveness, and can non-destructively detect trace amounts of free radicals and paramagnetic substances. Since there are no unpaired electrons in serum albumin, serum albumin is combined with a spin probe to form a conjugate, so that the conjugate carries unpaired electrons, that is, it carries free radicals. Based on electron paramagnetic resonance technology, spin probes bound to serum albumin and free spin probes can be detected.

[0057] In S300, since the waveforms of the spin probe bound to serum albumin and the free spin probe are different in the EPR spectrum, the waveforms can be identified to obtain a test signal representing the spin probe in a bound state and a test signal representing the spin probe in a free state. The spin probe in a bound state is a spin probe bound to serum albumin, and the spin probe in a free state is a spin probe not bound to serum albumin. The spin probe in a bound state and the spin probe in a free state in the test mixture are in different proportions, corresponding to different waveforms presented in the first detection spectrum. By comparing the first detection spectrum with the preset spectrum feature, the detection information representing the ratio of the spin probe in a bound state and the spin probe in a free state can be obtained, and the functional evaluation results of serum albumin in the test sample can be generated through this detection information. The functional evaluation results can reflect the ability of serum albumin in the test sample to bind to the detection molecule. A greater ability of serum albumin to bind to the detection molecule means that there is more serum albumin under physiological conditions in the test sample, the structure of the binding site in serum albumin is normal, and the transport ability and activity of serum albumin are better.

[0058] Cirrhosis is a chronic liver disease characterized by diffuse fibrosis, pseudolobules and regenerative nodule formation in liver tissue. Since serum albumin is mainly synthesized in the liver, when the disease develops into the cirrhosis stage, the liver's synthetic function declines, resulting in a significant decrease in serum albumin concentration in its plasma, and the structure of serum albumin also changes (including a variety of post-translational modifications), resulting in a decrease in serum albumin binding, transportation, detoxification and other functions. Therefore, the functional evaluation results of serum albumin provided in this application can also be used to evaluate the liver function of the user who provides the sample to be tested, for example: the liver function of patients with cirrhosis.

[0059] Serum albumin has an immunomodulatory effect, can inhibit inflammatory response, reduce oxidative stress, and enhance the body's resistance to bacteria and viruses. Therefore, the functional evaluation results of serum albumin provided in this application can also be used to evaluate the strength of human resistance.

[0060] Malnutrition can cause low serum albumin. Therefore, the functional evaluation results of serum albumin provided in this application can also be used to evaluate the nutritional status of the human body.

[0061] In some embodiments, before S300, the process includes:

[0062] S210, controlling the electron paramagnetic resonance spectrometer to detect multiple reference samples using the first working parameter to obtain second detection spectra corresponding to different reference samples;

[0063] S220, generating a preset spectrum feature for the first magnetic field region according to the ratio of the spin probes in a bound state to the spin probes in a free state in the reference sample and the second detection spectrum;

[0064] In S300, the first detection spectrum is compared with the preset spectrum characteristics, and the detection information that meets the preset spectrum characteristics is obtained, including:

[0065] S230, comparing the first detection spectrum obtained based on the first working parameter with the preset spectrum characteristics to obtain detection information that meets the preset spectrum characteristics and is located in the first magnetic field area.

[0066] The reference sample is a known ratio of the spin probe in the bound state to the spin probe in the free state, that is, the second detection spectrum corresponding to the different ratios is obtained. In S220, the preset spectrum feature is a waveform feature obtained by analyzing the second detection spectrum of several reference samples in advance. The preset spectrum feature can be obtained manually based on the second detection spectrum analysis of several reference samples, or can be obtained by peak search through analysis and testing software, or can be learned based on neural network technology.

[0067] If both free-state spin probes and bound-state spin probes exist in the sample to be tested, and the two overlap in the waveform display of the first detection spectrum, the band reflecting the ratio of the bound-state spin probes to the free-state spin probes in the waveform of the first detection spectrum can be identified by comparing with the preset spectrum features, and the band can be analyzed to obtain the detection information.

[0068] The electron paramagnetic resonance spectrometer can be controlled to use the same working parameters to test multiple solutions containing spin probes, so that the detected spectra have high similarity.

[0069] In some embodiments, the preset spectrogram features include:

[0070] (1) It has a triplet peak, and the coupling splitting interval value is (13G~17G);

[0071] (2) The g factor of the signal center is (1.96~2.04).

[0072] The detection information represented in the first detection spectrum can be identified by the preset spectrum features. Optionally, the detection information is generated by the difference between the peak and the trough of the third multiplet. The larger the difference between the peak and the trough of the third multiplet, the smaller the ratio of the spin probe in the bound state and the spin probe in the free state in the generated detection information; the smaller the difference between the peak and the trough of the third multiplet, the larger the ratio of the spin probe in the bound state and the spin probe in the free state in the generated detection information. Optionally, the third multiplet is in the first magnetic field region with a magnetic field strength of 3370 to 3380.

[0073] In some embodiments, the first operating parameters are: the dimensionless factor of the central magnetic field is 1.96-2.04, the scanning width is 50-500G, the modulation amplitude is 0.01-20G, the time constant is 0.01-1s, the microwave power is 0.02-200mW, and the test temperature is less than or equal to 40°C.

[0074] Optionally, the first operating parameters are: the dimensionless factor of the central magnetic field is 1.96, the scanning width is 50G, the modulation amplitude is 0.01G, the time constant is 0.01s, the microwave power is 0.02mW, and the test temperature is less than or equal to 40°C.

[0075] Optionally, the first operating parameters are: a dimensionless factor of a central magnetic field of 2.04, a scanning width of 500G, a modulation amplitude of 20G, a time constant of 1s, a microwave power of 200mW, and a test temperature less than or equal to 40°C.

[0076] Optionally, the first operating parameters are: a dimensionless factor of a central magnetic field of 2, a scanning width of 100G, a modulation amplitude of 10G, a time constant of 0.5s, a microwave power of 100mW, and a test temperature less than or equal to 40°C.

[0077] In some embodiments, the spin probe is one of stearic acid and its diffractors, stearate esters and their diffractors.

[0078] Stearic acid and its diffractors, stearic acid esters and their diffractors have stable structures and are easy to prepare. Serum albumin has binding sites for stearic acid and its diffractors, stearic acid esters and their diffractors, so that serum albumin can stably bind to stearic acid and its diffractors, stearic acid esters and their diffractors under physiological conditions.

[0079] In some embodiments, the radical with an unpaired electron in the spin probe is a nitroxide radical.

[0080] Nitrogen oxide free radicals are stable and are not prone to unwanted chemical reactions with the sample to be tested.

[0081] In some embodiments, the spin probe comprises any one of 5-nitroxy stearic acid or 16-nitroxy stearic acid, 5-nitroxy stearate, 16-nitroxy stearate.

[0082] In some embodiments, S110 includes:

[0083] The first solution is mixed with the sample to be tested to obtain a mixture to be tested, wherein the first solution includes a first solvent and a spin probe dissolved in the first solvent, and the first solvent is dimethyl sulfoxide or ethanol.

[0084] The spin probe itself cannot be directly dissolved in the serum extract containing serum albumin. Dimethyl sulfoxide or ethanol is used to dissolve the spin probe in the sample to be tested. Preferably, the first solvent is dimethyl sulfoxide, which may affect the activity of serum albumin compared to alcohol solvents such as ethanol, and cannot accurately reflect the ability of serum albumin in the sample to be tested to bind to the spin probe. Using dimethyl sulfoxide can not only be used as a solvent to dissolve the spin probe, but also has little effect on the structure of serum albumin.

[0085] In some embodiments, in the test mixture, the molar ratio of the spin probe to the serum albumin in the test sample is (1:1) to (7:1).

[0086] The concentration of serum albumin in the sample to be tested can be detected first to obtain the molar number of serum albumin per unit volume, and then the molar number of the added spin probe can be determined according to the molar number of serum albumin.

[0087] Since serum albumin has 7 stearic acid binding sites, ideally, when the molar ratio of serum albumin in the sample to be tested is 7:1, all 7 sites in serum albumin can bind to stearic acid, so that there is no free spin probe in the test mixture. If the binding function of some sites of serum albumin fails, free spin probes will exist in the test mixture.

[0088] Hereinafter, the method for evaluating the function of serum albumin of the present application will be described in more detail through examples, but the present application is not limited to these examples at all.

[0089] Embodiment 1:

[0090] 16-nitrogen oxide free radical stearic acid was selected as a spin probe, and 16-nitrogen oxide free radical stearic acid was dissolved in dimethyl sulfoxide to prepare a fatty acid solution with a spin probe. 20 μL of the prepared fatty acid solution was drawn with a capillary and placed in the resonant cavity of an electron paramagnetic resonance spectrometer for testing. The working parameters of the electron paramagnetic resonance spectrometer were set as follows:

[0091] The central magnetic field was g factor = 2.00, the sweep width was 120 G, the modulation amplitude was 1 G, the time constant was 0.1 s, the microwave power was 1 mW, and the test temperature was 25°C.

[0092] The EPR spectrum obtained by detection is as follows Figure 2 As shown, the central g factor of the signal is 2.034 and the hyperfine coupling splitting value is 15G.

[0093] Embodiment 2:

[0094] A blood sample was extracted by venipuncture from the tested user A with normal liver function. 1 mL of the blood sample was centrifuged at 4°C for 10 min at a centrifugal speed of 3000 r / min. After centrifugation, the upper serum was extracted and stored at -80°C.

[0095] 16-nitrogen oxide stearic acid was selected as a spin probe, and 16-nitrogen oxide stearic acid was dissolved in dimethyl sulfoxide to prepare a fatty acid solution with a spin probe. The test mixture was prepared according to the molar ratio of 16-nitrogen oxide stearic acid to serum albumin of 4:1, and 20 μL of the prepared test mixture was drawn with a capillary and placed in the resonant cavity of an electron paramagnetic resonance spectrometer for testing. The working parameters of the electron paramagnetic resonance spectrometer were set as follows:

[0096] The central magnetic field was g factor = 2.00, the sweep width was 120 G, the modulation amplitude was 1 G, the time constant was 0.1 s, the microwave power was 1 mW, and the test temperature was 25°C.

[0097] The EPR spectrum obtained by detection is as follows Figure 3 As shown, the waveform in the spectrum represents the waveform of the binding state of serum albumin and the spin probe, and no obvious signal of the free spin probe is observed.

[0098] Example 3

[0099] A blood sample was extracted by venipuncture from the tested user B with liver cirrhosis. 1 mL of the blood sample was centrifuged at 4°C for 10 min at a centrifugal speed of 3000 r / min. After centrifugation, the upper serum was extracted and stored at -80°C.

[0100] 16-nitrogen oxide stearic acid was selected as a spin probe, and 16-nitrogen oxide stearic acid was dissolved in dimethyl sulfoxide to prepare a fatty acid solution with a spin probe. A test mixture was prepared according to a molar ratio of 16-nitrogen oxide stearic acid to serum albumin of 4:1, and 20 μL of the prepared test mixture was drawn with a capillary and placed in the resonant cavity of an electron paramagnetic resonance spectrometer for testing.

[0101] The EPR parameters are set as follows:

[0102] The central magnetic field was g factor = 2.00, the sweep width was 120 G, the modulation amplitude was 1 G, the time constant was 0.1 s, the microwave power was 1 mW, and the test temperature was 25°C.

[0103] Test results such as Figure 4 As shown, the EPR spectrum is an overlay of the EPR signal of the bound state of serum albumin and the spin probe and the EPR signal of the free state spin probe, wherein the central g factor of the free state spin probe EPR signal is 2.034, and the hyperfine coupling splitting value is 15G.

[0104] Example 4

[0105] Mixtures with different ratios of bound spin probes and free spin probes were prepared, and the mixtures were placed in the resonant cavity of an electron paramagnetic resonance spectrometer for testing. The EPR parameters were set as follows:

[0106] The central magnetic field was g factor = 2.00, the sweep width was 120 G, the modulation amplitude was 1 G, the time constant was 0.1 s, the microwave power was 1 mW, and the test temperature was 25°C.

[0107] Test results such as Figure 5 As shown, from top to bottom, there are EPR spectra with the ratio of the spin probe in the bound state to the spin probe in the free state being 50%, 25%, 15%, 10%, 5%, and 0%. It can be observed that the EPR spectra corresponding to different ratios present different waveforms, and thus the detection information is obtained by analyzing the waveforms.

[0108] The serum albumin function evaluation method provided in the embodiment of the present application can be performed by a serum albumin function evaluation device. In the embodiment of the present application, the serum albumin function evaluation device performing the serum albumin function evaluation method is taken as an example to illustrate the serum albumin function evaluation device provided in the embodiment of the present application.

[0109] See also Figure 6 , Figure 6 FIG. 1 is a schematic diagram of a functional evaluation device for serum albumin provided in another embodiment of the present application. Figure 6 As shown, the serum albumin function assessment device may include:

[0110] A mixing module 601 is used to mix the spin probe with the sample to be tested to obtain a mixture to be tested, wherein the spin probe is stearic acid with free radicals;

[0111] The first testing module 602 is used to test the test mixture based on the electron paramagnetic resonance technology to obtain a first test signal representing the spin probe in a bound state and a second test signal representing the spin probe in a free state, wherein the serum albumin in the test sample is combined with the spin probe to form a spin probe in a bound state;

[0112] The first analysis module 603 is used to obtain a functional evaluation result of serum albumin in the sample to be tested according to the first test signal and the second test signal.

[0113] In some embodiments, the functional assessment device comprises:

[0114] A mixing module, which mixes the spin probe with the sample to be tested to obtain a mixture to be tested, wherein the spin probe is a detection molecule of a free radical with unpaired electrons, and the detection molecule is used to bind to a target site of serum albumin in the sample to be tested;

[0115] A first detection module is used to detect the mixture to be detected based on electron paramagnetic resonance technology to obtain a first detection spectrum;

[0116] A first comparison module, used for comparing the first detection spectrum with a preset spectrum feature to obtain detection information that meets the preset spectrum feature, wherein the detection information represents the ratio of the spin probe in a bound state to the spin probe in a free state;

[0117] The first analysis module 603 is used to generate a functional evaluation result of serum albumin in the sample to be tested according to the detection information.

[0118] In some embodiments, the functional assessment device further comprises:

[0119] A second detection module is used to control the electron paramagnetic resonance spectrometer to detect multiple reference samples using the first working parameter to obtain second detection spectra corresponding to different reference samples;

[0120] A second analysis module, for generating a preset spectrum feature about the first magnetic field region according to the ratio of the spin probes in the bound state to the spin probes in the free state in the reference sample and a second detection spectrum corresponding to the reference sample;

[0121] The first comparison module is also used to compare the first detection spectrum obtained based on the first working parameter with the preset spectrum characteristics to obtain detection information that meets the preset spectrum characteristics and is located in the first magnetic field area.

[0122] In some embodiments, the preset spectrogram features include:

[0123] (1) It has a triplet peak, and the coupling splitting interval value is (13G~17G);

[0124] (2) The g factor of the signal center is (1.96~2.04).

[0125] In some embodiments, the first operating parameters are: the dimensionless factor of the central magnetic field is 1.96-2.04, the scanning width is 50-500G, the modulation amplitude is 0.01-20G, the time constant is 0.01-1s, the microwave power is 0.02-200mW, and the test temperature is less than or equal to 40°C.

[0126] In some embodiments, the spin probe is one of stearic acid and its diffractors, stearate esters and their diffractors.

[0127] In some embodiments, the radical with an unpaired electron in the spin probe is a nitroxide radical.

[0128] In some embodiments, the spin probe comprises any one of 5-nitroxy stearic acid, 16-nitroxy stearic acid, 5-nitroxy stearate, and 16-nitroxy stearate.

[0129] In some embodiments, the spin probe is mixed with a sample to be tested to obtain a test mixture, comprising:

[0130] The first solution is mixed with the sample to be tested to obtain a mixture to be tested, wherein the first solution includes a first solvent and a spin probe dissolved in the first solvent, and the first solvent is dimethyl sulfoxide or ethanol.

[0131] In some embodiments, in the test mixture, the molar ratio of the spin probe to the serum albumin in the test sample is (1:1) to (7:1). Preferably, the molar ratio of the spin probe to the serum albumin in the test sample is (1:1), (2:1), (4:1), or (7:1).

[0132] The present application also provides a serum albumin function assessment kit, comprising:

[0133] The first solution is used to mix with the sample to be tested. The first solution includes a first solvent and a spin probe dissolved in the first solvent. The spin probe is a detection molecule of a free radical with unpaired electrons. The detection molecule is used to bind to a target site of serum albumin in the sample to be tested.

[0134] In the serum albumin function evaluation kit, the first solvent is dimethyl sulfoxide or ethanol.

[0135] The spin probes include 5-nitroxy stearic acid or 16-nitroxy stearic acid, 5-nitroxy stearate ester and 16-nitroxy stearate ester.

[0136] Figure 7 A schematic diagram of the hardware structure of a serum albumin function evaluation device provided in an embodiment of the present application is shown.

[0137] The serum albumin function assessment device may include a processor 101 and a memory 102 storing computer program instructions.

[0138] Specifically, the processor 101 may include a central processing unit (CPU), or an application specific integrated circuit (ASIC), or may be configured to implement one or more integrated circuits of the embodiments of the present application.

[0139] The memory 102 may include a large capacity memory for data or instructions. By way of example and not limitation, the memory 102 may include a hard disk drive (HDD), a floppy disk drive, a flash memory, an optical disk, a magneto-optical disk, a magnetic tape, or a universal serial bus (USB) drive or a combination of two or more of these. In appropriate cases, the memory 102 may include a removable or non-removable (or fixed) medium. In appropriate cases, the memory 102 may be inside or outside the integrated gateway disaster recovery device. In a specific embodiment, the memory 102 is a non-volatile solid-state memory.

[0140] The memory may include read-only memory (ROM), random access memory (RAM), magnetic disk storage media devices, optical storage media devices, flash memory devices, electrical, optical or other physical / tangible memory storage devices. Thus, typically, the memory includes one or more tangible (non-transitory) computer-readable storage media (e.g., memory devices) encoded with software including computer-executable instructions, and when the software is executed (e.g., by one or more processors), it is operable to perform the operations described with reference to the method according to an aspect of the present disclosure.

[0141] The processor 101 reads and executes the computer program instructions stored in the memory 102 to implement any one of the serum albumin function evaluation methods in the above embodiments.

[0142] In one example, the function evaluation device of serum albumin may further include a communication interface 1003 and a bus 1010. The processor 101, the memory 102, and the communication interface 1003 are connected via the bus 1010 and communicate with each other.

[0143] The communication interface 1003 is mainly used to implement communication between various modules, devices, units and / or equipment in the embodiments of the present application.

[0144] Bus 1010 includes hardware, software or both, and the parts of the functional evaluation equipment of serum albumin are coupled to each other. For example, but not limitation, bus may include accelerated graphics port (AGP) or other graphics bus, enhanced industrial standard architecture (EISA) bus, front side bus (FSB), hypertransport (HT) interconnection, industrial standard architecture (ISA) bus, infinite bandwidth interconnection, low pin count (LPC) bus, memory bus, micro channel architecture (MCA) bus, peripheral component interconnection (PCI) bus, PCI-Express (PCI-X) bus, serial advanced technology attachment (SATA) bus, video electronics standard association local (VLB) bus or other suitable bus or two or more of these combinations. In suitable cases, bus 1010 may include one or more buses. Although the present application embodiment describes and shows a specific bus, the application considers any suitable bus or interconnection.

[0145] The serum albumin function evaluation device can be based on the above embodiment, so as to implement the above serum albumin function evaluation method.

[0146] In addition, in combination with the functional evaluation method of serum albumin in the above-mentioned embodiment, the embodiment of the present application may provide a computer storage medium to implement. The computer storage medium stores computer program instructions; when the computer program instructions are executed by the processor, any one of the functional evaluation methods of serum albumin in the above-mentioned embodiments is implemented, and the same technical effect can be achieved. To avoid repetition, it is not repeated here. Among them, the above-mentioned computer-readable storage medium may include a non-transitory computer-readable storage medium, such as a read-only memory (Read-Only Memory, referred to as ROM), a random access memory (Random Access Memory, referred to as RAM), a disk or an optical disk, etc., which is not limited here.

[0147] In addition, an embodiment of the present application also provides a computer program product, including computer program instructions, which can implement the steps and corresponding contents of the aforementioned method embodiment when executed by a processor.

[0148] It should be clear that the present application is not limited to the specific configuration and processing described above and shown in the figures. For the sake of simplicity, a detailed description of the known method is omitted here. In the above embodiments, several specific steps are described and shown as examples. However, the method process of the present application is not limited to the specific steps described and shown, and those skilled in the art can make various changes, modifications and additions, or change the order between the steps after understanding the spirit of the present application.

[0149] The functional blocks shown in the above block diagram can be implemented as hardware, software, firmware or a combination thereof. When implemented in hardware, it can be, for example, an electronic circuit, an application specific integrated circuit (ASIC), appropriate firmware, a plug-in, a function card, etc. When implemented in software, the elements of the present application are programs or code segments that are used to perform the required tasks. The program or code segment can be stored in a machine-readable medium, or transmitted on a transmission medium or a communication link by a data signal carried in a carrier wave. "Machine-readable medium" can include any medium capable of storing or transmitting information. Examples of machine-readable media include electronic circuits, semiconductor memory devices, ROM, flash memory, erasable ROM (EROM), floppy disks, CD-ROMs, optical disks, hard disks, optical fiber media, radio frequency (RF) links, etc. The code segment can be downloaded via a computer network such as the Internet, an intranet, etc.

[0150] It should also be noted that the exemplary embodiments mentioned in this application describe some methods or systems based on a series of steps or devices. However, this application is not limited to the order of the above steps, that is, the steps can be performed in the order mentioned in the embodiment, or in a different order from the embodiment, or several steps can be performed simultaneously.

[0151] Aspects of the present disclosure are described above with reference to the flowchart and / or block diagram of the method, device and computer program product according to the embodiment of the present disclosure. It should be understood that each box in the flowchart and / or block diagram and the combination of each box in the flowchart and / or block diagram can be implemented by computer program instructions. These computer program instructions can be provided to a processor of a general-purpose computer, a special-purpose computer, or other programmable data processing device to produce a machine so that these instructions executed by the processor of the computer or other programmable data processing device enable the implementation of the function / action specified in one or more boxes of the flowchart and / or block diagram. Such a processor can be, but is not limited to, a general-purpose processor, a special-purpose processor, a special application processor, or a field programmable logic circuit. It can also be understood that each box in the block diagram and / or flowchart and the combination of boxes in the block diagram and / or flowchart can also be implemented by dedicated hardware that performs a specified function or action, or can be implemented by a combination of dedicated hardware and computer instructions.

[0152] The above are only specific implementation methods of the present application. Those skilled in the art can clearly understand that for the convenience and simplicity of description, the specific working processes of the systems, modules and units described above can refer to the corresponding processes in the aforementioned method embodiments, and will not be repeated here. It should be understood that the protection scope of the present application is not limited to this. Any technician familiar with the technical field can easily think of various equivalent modifications or replacements within the technical scope disclosed in this application, and these modifications or replacements should be included in the protection scope of this application.

Claims

1. A method for evaluating the function of serum albumin, characterized in that: The functional assessment method comprises: Mixing a spin probe with a sample to be tested to obtain a test mixture, wherein the spin probe is a detection molecule of a free radical having unpaired electrons, and the detection molecule is used to bind to a target site of serum albumin in the sample to be tested; Detecting the mixture to be tested based on electron paramagnetic resonance technology to obtain a first detection spectrum; The first detection spectrum is compared with a preset spectrum feature to obtain detection information that conforms to the preset spectrum feature, wherein the detection information represents the ratio of the spin probe in a bound state to the spin probe in a free state, and a functional evaluation result of serum albumin in the sample to be tested is generated according to the detection information.

2. The function evaluation method according to claim 1, characterized in that: Before comparing the first detected spectrum with the preset spectrum feature, the method includes: Controlling the electron paramagnetic resonance spectrometer to detect multiple reference samples using the first working parameter to obtain second detection spectra corresponding to different reference samples; generating a preset spectrum feature for the first magnetic field region according to the ratio of the spin probe in the bound state to the spin probe in the free state in the reference sample and a second detection spectrum corresponding to the reference sample; The comparing the first detection spectrum with the preset spectrum characteristics to obtain detection information that meets the preset spectrum characteristics includes: The first detection spectrum obtained based on the first working parameter is compared with a preset spectrum feature to obtain detection information that meets the preset spectrum feature and is located in the first magnetic field area.

3. The function evaluation method according to claim 2, characterized in that: The preset spectrogram features include: (1) It has a triplet peak, and the coupling splitting interval value is (13G~17G); (2) The g factor of the signal center is (1.96~2.04).

4. The function evaluation method according to claim 2, characterized in that: The first working parameters are: the dimensionless factor of the central magnetic field is 1.96-2.04, the scanning width is 50-500G, the modulation amplitude is 0.01-20G, the time constant is 0.01-1s, the microwave power is 0.02-200mW, and the test temperature is less than or equal to 40°C.

5. The function evaluation method according to claim 1, characterized in that: The spin probe is one of stearic acid and its diffractor, stearic acid ester and its diffractor.

6. The function evaluation method according to claim 1, characterized in that: The free radical with unpaired electrons in the spin probe is a nitroxide free radical.

7. The function evaluation method according to any one of claims 5 or 6, characterized in that: The spin probe includes any one of 5-nitroxy stearic acid, 16-nitroxy stearic acid, 5-nitroxy stearate, and 16-nitroxy stearate.

8. The function evaluation method according to claim 1, characterized in that: The step of mixing the spin probe with the sample to be tested to obtain the mixture to be tested comprises: A first solution is mixed with the sample to be tested to obtain a mixture to be tested, wherein the first solution includes a first solvent and the spin probe dissolved in the first solvent, and the first solvent is dimethyl sulfoxide or ethanol.

9. The function evaluation method according to claim 1, characterized in that: In the test mixture, the molar ratio of the spin probe to the serum albumin in the test sample is (1:1) to (7:1).

10. A serum albumin function evaluation device, characterized in that: include: A mixing module, mixing the spin probe with the sample to be tested to obtain a mixture to be tested, wherein the spin probe is a detection molecule of a free radical with unpaired electrons, and the detection molecule is used to bind to a target site of serum albumin in the sample to be tested; A first detection module, used to detect the mixture to be tested based on electron paramagnetic resonance technology to obtain a first detection spectrum; A first comparison module, used for comparing the first detection spectrum with a preset spectrum feature to obtain detection information that conforms to the preset spectrum feature, wherein the detection information represents a ratio of the spin probe in a bound state to the spin probe in a free state; The first analysis module is used to generate a functional evaluation result of serum albumin in the sample to be tested according to the detection information.

11. A kit for evaluating the function of serum albumin, characterized in that: include: A first solution is used to mix with the sample to be tested, wherein the first solution includes a first solvent and the spin probe dissolved in the first solvent, wherein the spin probe is a detection molecule of a free radical with unpaired electrons, and the detection molecule is used to bind to a target site of serum albumin in the sample to be tested.

12. A serum albumin function evaluation device, characterized in that: The method comprises a processor and a memory, wherein the memory stores a program or instruction that can be run on the processor, and when the program or instruction is executed by the processor, the steps of the method for evaluating the function of serum albumin according to any one of claims 1 to 9 are implemented.

13. A readable storage medium, characterized in that: The readable storage medium stores a program or instruction, and when the program or instruction is executed by a processor, the steps of the method for evaluating the function of serum albumin according to any one of claims 1 to 9 are implemented.

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