Method for detecting nano plastic in sperm or follicular fluid based on Py-GCMS

Abstract

The invention relates to a method for detecting nano-plastics in semen or follicular fluid based on Py-GCMS, the nano-plastics can be separated and purified through optimization of pretreatment steps, the content and the variety of the nano-plastics in the semen or the follicular fluid can be quantitatively analyzed through gas chromatography-mass spectrometry contrastive analysis, and the content and the variety of the nano-plastics in the semen or the follicular fluid can be accurately detected by combining with a Py-GCMS detection system. The content of the nano plastic in the semen / follicular fluid is quantitatively analyzed and monitored, a corresponding curve is constructed through quantitative analysis, the content is visually displayed, and a user can visually check and compare data in combination with a terminal and the like.

Description

Technical Field

[0001] The present disclosure relates to the field of biological detection technology, and in particular to a method and system for detecting nanoplastics in sperm or follicular fluid based on Py-GCMS. Background Art

[0002] Microplastics (micron-sized plastics + nano-sized plastics) are widely present in the environment and have become a source of pollution that cannot be ignored. Among them, nanoplastics are more harmful because they can easily enter tissue cells due to their tiny size and have a direct effect on cells. However, the detection of nanoplastics has always been a difficult point in the industry. First, the resolution of the detection instrument is limited, and it is difficult to detect nano-sized particles. Second, nanoplastics are small in size and low in content, and are easily lost during conventional pre-treatment. Third, nanoplastics are mixed with other micron-sized plastics and are difficult to separate. The detected data cannot truly reflect the content of nanoplastics.

[0003] The presence of microplastics has been proven in multiple tissues and organs of the human body, but it is still unknown whether nanoplastics exist and how much they contain.

[0004] The Py-GCMS detection and analysis technology can perform gas chromatography analysis of materials through thermal cracking technology to identify the content and type of materials. Therefore, it can be used to detect nanoplastics in semen or follicular fluid. Summary of the invention

[0005] In order to solve the above problems, the present application proposes a method and system for detecting nanoplastics in sperm or follicular fluid based on Py-GCMS.

[0006] On the one hand, the present application proposes a method for detecting nanoplastics in sperm or follicular fluid based on Py-GCMS, comprising the following steps:

[0007] preparing semen or follicular fluid and performing pre-treatment to obtain a pre-treated sample;

[0008] Based on Py-GCMS, the pre-treated sample is tested and a mass spectrum corresponding to each component in the pre-treated sample is constructed;

[0009] Constructing a nanoplastic standard of semen or follicular fluid, and constructing a quantitative curve of the nanoplastic standard based on Py-GCMS;

[0010] The mass spectrum is quantitatively analyzed, compared and calculated with the quantitative curve of the nanoplastic standard to determine the content of nanoplastics in semen or follicular fluid.

[0011] As an optional embodiment of the present application, optionally, in the step of pre-treating semen or follicular fluid to obtain a pre-treated sample:

[0012] If the pre-treatment sample is semen, the pre-treatment process is as follows:

[0013] Weigh the semen sample quantitatively into a centrifuge tube, add enzyme solution to mix it with the sample thoroughly, put it into a 37°C water bath for incubation and then take it out; centrifuge it in a centrifuge, filter the supernatant, take the sample under the membrane and dry it in an oven to constant weight;

[0014] Extraction: directly add chloroform solution to the sample beaker, ultrasonicate three times, and transfer the extract to a new beaker; add hexafluoroisopropanol solution to extract the sample, ultrasonicate three times, and pour the extract into the beaker; finally, extract the sample with xylene solution, heat it, and pour the extract into the beaker;

[0015] Concentration and testing: Concentrate the extract and drip it into the sampling crucible of Py-GCMS using a glass pipette. After the solvent in the crucible is completely evaporated, test it on the machine.

[0016] As an optional embodiment of the present application, optionally, in the step of pre-treating semen or follicular fluid to obtain a pre-treated sample:

[0017] If the pre-treatment sample is follicular fluid, the pre-treatment process is as follows:

[0018] Quantitatively weigh the follicular fluid sample into a centrifuge tube, centrifuge it in a centrifuge, filter the supernatant, and dry it in an oven to constant weight;

[0019] Extraction: Add chloroform solution directly to the sample beaker, sonicate three times, and transfer the extract to a new beaker; then add hexafluoroisopropanol solution to extract the sample, sonicate three times, and pour the extract into a beaker; finally, extract the sample with xylene solution, heat it, and pour the extract into a beaker;

[0020] Concentration and testing: Concentrate the extract and drip it into the sampling crucible of Py-GCMS using a glass pipette. After the solvent in the crucible is completely evaporated, test it on the machine.

[0021] As an optional embodiment of the present application, optionally, based on Py-GCMS, the pre-treated sample is tested, and a mass spectrum corresponding to each component in the pre-treated sample is constructed, including:

[0022] The pre-treated sample is placed in a cracking furnace of a Py-GCMS system for high temperature cracking;

[0023] The gas components generated by the cracking are separated by gas chromatography through a gas chromatography column to form different chromatographic peaks. The separated gas components enter a mass spectrometer detector to obtain a first mass spectrum of each component in the pre-treated sample.

[0024] As an optional embodiment of the present application, optionally, a nanoplastic standard of semen or follicular fluid is constructed, and a quantitative curve of the nanoplastic standard is constructed based on Py-GCMS, comprising:

[0025] Nanoplastic standards with a quantitative percentage in semen or follicular fluid;

[0026] The nanoplastic standard is placed in a pyrolysis furnace of a Py-GCMS system for high temperature pyrolysis;

[0027] The gas components generated by the pyrolysis are separated by gas chromatography through a gas chromatography column to form different chromatographic peaks. The separated gas components enter a mass spectrometer detector to obtain a second mass spectrum of each component in the nanoplastic standard product.

[0028] According to the chromatographic features on the second mass spectrum, a quantitative curve A of the nanoplastic standard in the nanoplastic standard is constructed.

[0029] As an optional embodiment of the present application, optionally, the mass spectrum is subjected to quantitative analysis and comparative calculation with the quantitative curve of the nanoplastic standard to determine the content of nanoplastics in semen or follicular fluid, including:

[0030] Constructing a quantitative curve B for each component in the pre-treated sample according to the chromatographic characteristics on the first mass spectrum of each component in the pre-treated sample;

[0031] A quantitative comparative analysis is performed on the quantitative curve A of the nanoplastic standard and the quantitative curve B of each component in the pre-treated sample under chromatographic characteristics to calculate the quantitative analysis results of each component in the pre-treated sample.

[0032] On the other hand, the present application proposes a system for implementing the method for detecting nanoplastics in sperm or follicular fluid based on Py-GCMS, comprising:

[0033] A pre-treatment system, used for preparing semen or follicular fluid and performing pre-treatment to obtain a pre-treated sample;

[0034] A Py-GCMS system is used to test the pre-treated sample and construct a mass spectrum corresponding to each component in the pre-treated sample; and a quantitative curve of the nanoplastic standard is constructed based on Py-GCMS;

[0035] The quantitative analysis system is used to perform quantitative analysis, comparison and calculation on the mass spectrum and the quantitative curve of the nanoplastic standard to determine the content of nanoplastics in semen or follicular fluid.

[0036] Technical effects of the present invention:

[0037] This application prepares semen or follicular fluid and performs pretreatment to obtain a pretreatment sample; based on Py-GCMS, the pretreatment sample is tested and a mass spectrum corresponding to each component in the pretreatment sample is constructed; a nanoplastic standard of semen or follicular fluid is constructed, and a quantitative curve of the nanoplastic standard is constructed based on Py-GCMS; the mass spectrum is quantitatively analyzed and compared with the quantitative curve of the nanoplastic standard to determine the content of nanoplastics in semen or follicular fluid. The optimization of the pretreatment step allows the nanoplastic to be separated and purified, and the content and type of nanoplastics in semen or follicular fluid can be quantitatively analyzed by gas phase mass spectrometry comparison analysis. Combined with the Py-GCMS detection system, the quantitative analysis and monitoring of the nanoplastic content in semen / follicular fluid are carried out, and the corresponding curve is constructed by quantitative analysis to visualize the content. It can be combined with terminals, etc., so that users can intuitively view the comparison data.

[0038] Further features and aspects of the present disclosure will become apparent from the following detailed description of exemplary embodiments with reference to the attached drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0039] The accompanying drawings, which are incorporated in and constitute a part of the specification, illustrate exemplary embodiments, features, and aspects of the disclosure and, together with the description, serve to explain the principles of the disclosure.

[0040] Figure 1 It is shown as a schematic diagram of the implementation process of the present invention;

[0041] Figure 2 Shown is a schematic diagram of mass spectra comparison of micron-scale plastic and nano-scale plastic of the present invention;

[0042] Figure 3 It is a schematic diagram of the system composition structure of the present invention. DETAILED DESCRIPTION

[0043] Various exemplary embodiments, features and aspects of the present disclosure will be described in detail below with reference to the accompanying drawings. The same reference numerals in the accompanying drawings represent elements with the same or similar functions. Although various aspects of the embodiments are shown in the accompanying drawings, the drawings are not necessarily drawn to scale unless otherwise specified.

[0044] The word “exemplary” is used exclusively herein to mean “serving as an example, example, or illustration.” Any embodiment described herein as “exemplary” is not necessarily to be construed as preferred or advantageous over other embodiments.

[0045] In addition, in order to better illustrate the present disclosure, numerous specific details are given in the following specific embodiments. It should be understood by those skilled in the art that the present disclosure can also be implemented without certain specific details. In some examples, means, components and circuits well known to those skilled in the art are not described in detail in order to highlight the main purpose of the present disclosure.

[0046] Example 1

[0047] like Figure 1 As shown, on the one hand, the present application proposes a method for detecting nanoplastics in sperm or follicular fluid based on Py-GCMS, comprising the following steps:

[0048] S1, preparing semen or follicular fluid, and performing pre-treatment to obtain a pre-treated sample;

[0049] S2. Based on Py-GCMS, the pre-treated sample is tested and a mass spectrum corresponding to each component in the pre-treated sample is constructed;

[0050] S3. constructing a nanoplastic standard of semen or follicular fluid, and constructing a quantitative curve of the nanoplastic standard based on Py-GCMS;

[0051] S4. Quantitatively analyze and compare the mass spectrum with the quantitative curve of the nanoplastic standard to determine the content of nanoplastics in semen or follicular fluid.

[0052] In order to separate and purify nanoplastics in semen or follicular fluid, a specific pre-treatment step is adopted to pre-treat semen or follicular fluid to obtain a pre-treated sample. Centrifugation, filtration and extraction are used in combination for purification.

[0053] Py-GCMS, or pyrolysis gas chromatography-mass spectrometry, is an analytical method for polymers, additives, coatings, fibers, elastomers, inks, paper fibers, rubber, wood and other materials. Its working principle is that in a helium atmosphere, polymers are instantly vaporized at high temperatures, the chemical bonds of the samples are broken, and they are fragmented into small molecular weight, volatile monomer components. These vaporized components after pyrolysis are separated by GC gas chromatography and enter the MS mass spectrometer. By qualitatively and quantitatively analyzing the mass spectra of the pyrolysis products, information such as the chemical structure, material composition, and chemical reaction process of unknown substances can be obtained.

[0054] Because the chemical compositions of micron-scale plastics and nano-scale plastics are different, the chromatographic peaks produced after phase separation are different. Therefore, chromatographic analysis can be performed by thermal cracking, and the mass spectrometry curve of the standard can be combined to perform quantitative analysis of nano-scale plastics.

[0055] When using the Py-GCMS detection system to distinguish between micron-sized plastics and nano-sized plastics, the following steps can be followed:

[0056] 1. Sample preparation: Pre-treat the micron-sized plastic and nano-sized plastic separately, such as drying and grinding, for subsequent cracking and detection.

[0057] 2. Pyrolysis process: The pre-treated samples are placed in the pyrolysis furnace of the Py-GCMS system for high-temperature pyrolysis. Due to the smaller size of nano-scale plastics, the pyrolysis process may be faster and more thorough.

[0058] 3. Gas chromatography separation: The gas components produced by pyrolysis are separated by a gas chromatography column to form different chromatographic peaks. Since the chemical composition of micro-scale plastics and nano-scale plastics may be different, their chromatographic peaks will also be different.

[0059] 4. Mass spectrometry: The separated gas components enter the mass spectrometer to obtain the mass spectra of each component. By comparing the mass spectra of micron-scale plastics and nano-scale plastics, their differences can be further confirmed.

[0060] 5. Data analysis: Based on the data of chromatogram and mass spectrum, qualitative and quantitative analysis of micron-scale plastics and nano-scale plastics can be performed. By comparing their characteristic peaks, peak intensity and other information, the differences in their composition and content can be determined.

[0061] Therefore, the method of distinguishing micron-sized plastics from nano-sized plastics using the Py-GCMS detection system mainly includes sample preparation, pyrolysis process, gas chromatography separation, mass spectrometry detection and data analysis. By comprehensively using these technical means, effective distinction and identification of micron-sized plastics and nano-sized plastics can be achieved.

[0062] like Figure 2 The following are the chromatograms of micron-sized plastic and nano-sized plastic under Py-GCMS detection. (a) is the chromatogram of micron-sized plastic, and (b) is the chromatogram of nano-sized plastic.

[0063] On the basis of the above, this plan further implements the following technologies:

[0064] 1. The semen pretreatment process is:

[0065] Weigh about 2g of sample into a centrifuge tube, add about 2g of 500u / ml enzyme solution to mix it with the sample thoroughly, put it in a 37℃ water bath for incubation for 10min, take it out, put it in a centrifuge, centrifuge at 3000r for 10min, take the supernatant and filter it with a 1um filter membrane, take the sample under the membrane and dry it in a 6℃ oven to constant weight.

[0066] Extraction: Add 10g of chloroform solution directly to the sample beaker, ultrasonicate for 10min, repeat three times, and transfer the extract to a new beaker B. Then add 10g of hexafluoroisopropanol solution to extract the sample, ultrasonicate for 10min, repeat three times, and pour the extract into beaker B. Finally, extract the sample with 10g of xylene solution, heat at 150℃ for 10min, repeat three times, and pour the extract into beaker B.

[0067] Concentration and testing: The extract was concentrated at 80°C to about 0.5 g, and was dripped into the sampling crucible of the Py-GCMS using a glass pipette. After the solvent in the crucible was completely evaporated, it was tested on the machine.

[0068] 2. The pre-treatment process of follicular fluid is as follows:

[0069] Weigh about 5 g of sample into a centrifuge tube, put it into a centrifuge, centrifuge at 3000r for 10 min, take the supernatant and filter it with a 1μm steel membrane. The sample under the membrane is about 2 g, and dry it in a 60℃ oven to constant weight.

[0070] Extraction: Add 10g of chloroform solution directly to the sample beaker, ultrasonicate for 10min, repeat three times, and transfer the extract to a new beaker B. Then add 10g of hexafluoroisopropanol solution to extract the sample, ultrasonicate for 10min, repeat three times, and pour the extract into beaker B. Finally, extract the sample with 10g of xylene solution, heat at 150℃ for 10min, repeat three times, and pour the extract into beaker B.

[0071] Concentration and testing: The extract was concentrated at 80°C to about 0.5 g, and was dripped into the sampling crucible of the Py-GCMS using a glass pipette. After the solvent in the crucible was completely evaporated, it was tested on the machine.

[0072] As an optional embodiment of the present application, optionally, based on Py-GCMS, the pre-treated sample is tested, and a mass spectrum corresponding to each component in the pre-treated sample is constructed, including:

[0073] The pre-treated sample is placed in a cracking furnace of a Py-GCMS system for high temperature cracking;

[0074] The gas components generated by the cracking are separated by gas chromatography through a gas chromatography column to form different chromatographic peaks. The separated gas components enter a mass spectrometer detector to obtain a first mass spectrum of each component in the pre-treated sample.

[0075] For the method of thermal cracking the pre-treated sample, please refer to the above-mentioned steps of "Using the Py-GCMS detection system to distinguish between micron-sized plastics and nano-sized plastics", which will not be described in detail in this embodiment.

[0076] The specific sample value can be determined by the user.

[0077] This solution prepares standard samples with different contents and conducts Py-GCMS testing to construct a quantitative curve. The quantitative curve is used for subsequent chromatographic quantitative analysis to identify and calculate the content and type of the corresponding nanoplastics.

[0078] As an optional embodiment of the present application, optionally, a nanoplastic standard of semen or follicular fluid is constructed, and a quantitative curve of the nanoplastic standard is constructed based on Py-GCMS, comprising:

[0079] Nanoplastic standards with a quantitative percentage in semen or follicular fluid;

[0080] The nanoplastic standard is placed in a pyrolysis furnace of a Py-GCMS system for high temperature pyrolysis;

[0081] The gas components generated by the pyrolysis are separated by gas chromatography through a gas chromatography column to form different chromatographic peaks. The separated gas components enter a mass spectrometer detector to obtain a second mass spectrum of each component in the nanoplastic standard product.

[0082] According to the chromatographic features on the second mass spectrum, a quantitative curve A of the nanoplastic standard in the nanoplastic standard is constructed.

[0083] After the nanoplastic standard is detected by mass spectrometry using Py-GCMS, a second mass spectrum of each component in the nanoplastic standard can be obtained.

[0084] At the same time, a quantitative curve A of the nanoplastic standard in the nanoplastic standard can be constructed according to the chromatographic characteristics on the second mass spectrum.

[0085] On the quantitative curve A, the chromatographic characteristics of each component in the nanoplastic standard can be used to compare and analyze the chromatographic comparative characteristics of nanoplastics in semen or follicular fluid, so as to determine the content and type of nanoplastics in semen or follicular fluid.

[0086] As an optional embodiment of the present application, optionally, the mass spectrum is subjected to quantitative analysis and comparative calculation with the quantitative curve of the nanoplastic standard to determine the content of nanoplastics in semen or follicular fluid, including:

[0087] Constructing a quantitative curve B for each component in the pre-treated sample according to the chromatographic characteristics on the first mass spectrum of each component in the pre-treated sample;

[0088] A quantitative comparative analysis is performed on the quantitative curve A of the nanoplastic standard and the quantitative curve B of each component in the pre-treated sample under chromatographic characteristics to calculate the quantitative analysis results of each component in the pre-treated sample.

[0089] For the pre-treated samples, a quantitative curve B of each component can also be constructed and generated.

[0090] By performing quantitative comparative analysis under chromatographic characteristics on the quantitative curve A of the nanoplastic standard and the quantitative curve B of each component in the pre-treated sample, the quantitative analysis results of each component in the pre-treated sample are calculated, including characteristic comparison of chromatographic peak peaks, the number of peak signals and the corresponding types, etc.

[0091] The comparison of specific chromatographic characteristics can be implemented in combination with the mass spectrometry detection principle of Py-GCMS.

[0092] Therefore, by performing a quantitative comparative analysis under chromatographic characteristics on the quantitative curve A of the nanoplastic standard and the quantitative curve B of each component in the pre-treated sample, the chromatographic characteristic comparison data on the mass spectrometer can be used to determine the nanoplastic detection in semen and follicular fluid, thereby facilitating the analysis of the quality of semen and follicular fluid.

[0093] Example 2

[0094] like Figure 3 As shown, based on the implementation principle of Example 1, on the other hand, the present application proposes a system for implementing the method of detecting nanoplastics in sperm or follicular fluid based on Py-GCMS, comprising:

[0095] A pre-treatment system, used for preparing semen or follicular fluid and performing pre-treatment to obtain a pre-treated sample;

[0096] A Py-GCMS system is used to test the pre-treated sample and construct a mass spectrum corresponding to each component in the pre-treated sample; and a quantitative curve of the nanoplastic standard is constructed based on Py-GCMS;

[0097] The quantitative analysis system is used to perform quantitative analysis, comparison and calculation on the mass spectrum and the quantitative curve of the nanoplastic standard to determine the content of nanoplastics in semen or follicular fluid.

[0098] The modules or steps of the present invention described above can be implemented by a general-purpose computing system, they can be concentrated on a single computing system, or distributed on a network composed of multiple computing systems, and optionally, they can be implemented by a program code executable by a computing system, so that they can be stored in a storage system and executed by the computing system, or they can be made into individual integrated circuit modules, or multiple modules or steps therein can be made into a single integrated circuit module for implementation. Thus, the present invention is not limited to any specific combination of hardware and software.

[0099] The embodiments of the present disclosure have been described above, and the above description is exemplary, not exhaustive, and is not limited to the disclosed embodiments. Many modifications and changes will be apparent to those of ordinary skill in the art without departing from the scope and spirit of the described embodiments. The selection of terms used herein is intended to best explain the principles of the embodiments, practical applications, or technical improvements in the market, or to enable other persons of ordinary skill in the art to understand the embodiments disclosed herein.

Claims

1. A method for detecting nanoplastics in sperm or follicular fluid based on Py-GCMS, characterized in that: The steps include: preparing semen or follicular fluid and performing pre-treatment to obtain a pre-treated sample; Based on Py-GCMS, the pre-treated sample is tested and a mass spectrum corresponding to each component in the pre-treated sample is constructed; Constructing a nanoplastic standard of semen or follicular fluid, and constructing a quantitative curve of the nanoplastic standard based on Py-GCMS; The mass spectrum is quantitatively analyzed, compared and calculated with the quantitative curve of the nanoplastic standard to determine the content of nanoplastics in semen or follicular fluid.

2. A method for detecting nanoplastics in sperm or follicular fluid based on Py-GCMS according to claim 1, characterized in that: In the step of pre-treating semen or follicular fluid to obtain a pre-treated sample: If the pre-treatment sample is semen, the pre-treatment process is as follows: Weigh the semen sample quantitatively into a centrifuge tube, add enzyme solution to mix it with the sample thoroughly, put it into a 37°C water bath for incubation and then take it out; centrifuge it in a centrifuge, filter the supernatant, take the sample under the membrane and dry it in an oven to constant weight; Extraction: Add chloroform solution directly into the sample beaker, sonicate three times, and transfer the extract into a new beaker; Add hexafluoroisopropanol solution to extract the sample, ultrasonicate three times, pour the extract into a beaker, and finally extract the sample with xylene solution, heat it, and pour the extract into a beaker; Concentration and testing: Concentrate the extract and drip it into the sampling crucible of Py-GCMS using a glass pipette. After the solvent in the crucible is completely evaporated, test it on the machine.

3. The method for detecting nanoplastics in sperm or follicular fluid based on Py-GCMS according to claim 1, characterized in that: In the step of pre-treating semen or follicular fluid to obtain a pre-treated sample: If the pre-treatment sample is follicular fluid, the pre-treatment process is as follows: Quantitatively weigh the follicular fluid sample into a centrifuge tube, centrifuge it in a centrifuge, filter the supernatant, and dry it in an oven to constant weight; Extraction: Add chloroform solution directly to the sample beaker, sonicate three times, and transfer the extract to a new beaker; then add hexafluoroisopropanol solution to extract the sample, sonicate three times, and pour the extract into a beaker; finally, extract the sample with xylene solution, heat it, and pour the extract into a beaker; Concentration and testing: Concentrate the extract and drip it into the sampling crucible of Py-GCMS using a glass pipette. After the solvent in the crucible is completely evaporated, test it on the machine.

4. The method for detecting nanoplastics in sperm or follicular fluid based on Py-GCMS according to claim 1, characterized in that: Based on Py-GCMS, the pre-treated sample is tested, and a mass spectrum corresponding to each component in the pre-treated sample is constructed, including: The pre-treated sample is placed in a cracking furnace of a Py-GCMS system for high temperature cracking; The gas components generated by the cracking are separated by gas chromatography through a gas chromatography column to form different chromatographic peaks. The separated gas components enter a mass spectrometer detector to obtain a first mass spectrum of each component in the pre-treated sample.

5. The method for detecting nanoplastics in sperm or follicular fluid based on Py-GCMS according to claim 1, characterized in that: Constructing a nanoplastic standard of semen or follicular fluid, and constructing a quantitative curve of the nanoplastic standard based on Py-GCMS, including: Nanoplastic standards with a quantitative percentage in semen or follicular fluid; The nanoplastic standard is placed in a pyrolysis furnace of a Py-GCMS system for high temperature pyrolysis; The gas components generated by the pyrolysis are separated by gas chromatography through a gas chromatography column to form different chromatographic peaks. The separated gas components enter a mass spectrometer detector to obtain a second mass spectrum of each component in the nanoplastic standard product. According to the chromatographic features on the second mass spectrum, a quantitative curve A of the nanoplastic standard in the nanoplastic standard is constructed.

6. The method for detecting nanoplastics in sperm or follicular fluid based on Py-GCMS according to claim 5, characterized in that: The mass spectrum is quantitatively analyzed, compared and calculated with the quantitative curve of the nanoplastic standard to determine the content of nanoplastics in semen or follicular fluid, including: Constructing a quantitative curve B for each component in the pre-treated sample according to the chromatographic characteristics on the first mass spectrum of each component in the pre-treated sample; A quantitative comparative analysis is performed on the quantitative curve A of the nanoplastic standard and the quantitative curve B of each component in the pre-treated sample under chromatographic characteristics to calculate the quantitative analysis results of each component in the pre-treated sample.

7. A system for implementing the method of detecting nanoplastics in sperm or follicular fluid based on Py-GCMS, characterized in that: include: A pre-treatment system, used for preparing semen or follicular fluid and performing pre-treatment to obtain a pre-treated sample; A Py-GCMS system is used to test the pre-treated sample and construct a mass spectrum corresponding to each component in the pre-treated sample; and a quantitative curve of the nanoplastic standard is constructed based on Py-GCMS; The quantitative analysis system is used to perform quantitative analysis, comparison and calculation on the mass spectrum and the quantitative curve of the nanoplastic standard to determine the content of nanoplastics in semen or follicular fluid.

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