Trunk cultivation method for paphiopedilum fortunei

Abstract

The invention discloses a paphiopedilum nobilis trunk cultivation method. Moss is used as an auxiliary plant to cultivate paphiopedilum nobilis on a trunk. According to the method, rapid rooting and survival of paphiopedilum fortunei seedlings are promoted through operations of trunk selection, auxiliary plant planting, seedling field planting, water and fertilizer management and the like. The problems that in paphiopedilum nobilis trunk cultivation, the fleshy root system is prone to breaking, the trunk is difficult to fix, the tissue culture seedling root system grows slowly, and the survival rate is low are solved, and the moss is creatively planted on the trunk as an auxiliary plant for the first time, so that successful cultivation of paphiopedilum nobilis is achieved. By means of the method, the survival rate of paphiopedilum nobilis tissue culture seedling trunks cultivated for one year can reach 85.5%. In addition, the growing space position of the paphiopedilum nobilis can be increased, the ornamental value is increased, a large number of trees in various parks can be fully utilized, a symbiotic system of the paphiopedilum nobilis and the trees is formed, the paphiopedilum nobilis can directly utilize water on the skin of the tree trunk, and the management cost is reduced. In conclusion, the method is easy to operate and master, good in effect and beneficial to application and popularization.

Description

Technical Field

[0001] The invention belongs to the technical field of Paphiopedilum leucophyllum cultivation, and in particular relates to a Paphiopedilum leucophyllum trunk cultivation method. Background Art

[0002] Paphiopedilum, also known as "slipper orchid" and "slipper orchid", is a general term for plants of the genus Paphiopedilum in the Orchidaceae family. Paphiopedilum has a unique flower shape, gorgeous colors, a long flowering period, and is of great ornamental value. Paphiopedilum, Cattleya, and Phalaenopsis constitute the three major mainstream orchids, and occupy an important position in the international high-end flower market, with an annual trade volume of billions of dollars and a very good market prospect. Paphiopedilum plants are mainly distributed in tropical Asia to some islands in the Pacific Ocean. So far, 88 wild species have been discovered and recorded, of which about 1 / 3 are distributed in China. Most wild Paphiopedilum plants grow on rock walls or soil. Among the 27 species of Paphiopedilum distributed in China, only a small number of Paphiopedilum villosum plants grow on tree trunks. The roots of Paphiopedilum plants are fleshy and thick, but they are not tough enough and are easily broken. They are very easy to be injured during cultivation. If you want to cultivate them on tree trunks, you must first solve the problem of easy root breakage. In summary, due to factors such as the native environment, growth pattern and root characteristics of the Paphiopedilum plant, artificial cultivation of Paphiopedilum is usually carried out in pots or in the ground. It is difficult to successfully plant Paphiopedilum on tree trunks, and there are no reports of cultivating Paphiopedilum on trees.

[0003] Paphiopedilum emersonii is a perennial plant of the genus Paphiopedilum in the Orchidaceae family. It is endemic to my country and a national first-class protected plant. Paphiopedilum emersonii has high ornamental value and is deeply loved by people, but its wild resources are scarce and endangered. According to surveys, the existing resources of Paphiopedilum emersonii are only about 280 plants in 7 populations in Guangxi Mulun Nature Reserve and about 306 plants in 6 populations in Guizhou Maolan Nature Reserve (Qin Wenyuan et al., 2012; Tan Weining, 2009). The number of populations and individuals is far below the minimum viable number of stable survival limits (the world-recognized herbaceous plant group is 10,000 individuals). Years of field surveys have found that Paphiopedilum emersonii basically grows on rock walls with a rock exposure of more than 90%, and its habitat is fragile and easily destroyed. In recent years, the artificial propagation and cultivation of Paphiopedilum emersonii seedlings have received widespread attention. Research has mainly focused on aseptic germination of seeds and container cultivation. Seedling tissue culture technology has made certain breakthroughs, but the cultivation method is single. In addition, Paphiopedilum whiteiflorum is a small herb. The current single potted or ground planting method places it in a lower spatial position, which cannot meet people's diverse appreciation requirements and greatly weakens the ornamental value of Paphiopedilum whiteiflorum. Summary of the invention

[0004] The technical problem to be solved by the present invention is to provide a method for cultivating white-flowered Paphiopedilum trunks with low cost, simple operation and high survival rate. The method promotes the rapid rooting and survival of white-flowered Paphiopedilum seedlings and increases the ornamental mode of white-flowered Paphiopedilum.

[0005] In order to solve the above technical problems, the present invention adopts the following technical solutions:

[0006] The invention discloses a method for cultivating white-flowered Paphiopedilum on a tree trunk, which utilizes moss as an auxiliary plant to cultivate white-flowered Paphiopedilum on the tree trunk.

[0007] The above-mentioned method for cultivating the white flowered Paphiopedilum trunk comprises the steps of planting moss on the trunk before planting the white flowered Paphiopedilum seedlings.

[0008] The mosses are living mosses and the trunks are trunks of evergreen trees.

[0009] Since wild Paphiopedilum leucodendron grows on rock walls with a rock exposure of more than 90%, it has developed fleshy roots and is more drought-resistant. However, the loose and breathable soil and the moist and non-waterlogged environment are conducive to the growth of Paphiopedilum leucodendron. The purpose of planting moss in the present invention is to create a moist and non-waterlogged environment and to fix the Paphiopedilum leucodendron plants and nutrient soil.

[0010] Although there are also reports of using mosses in daily Paphiopedilum cultivation, the mosses used are all dry dead mosses, which cover the surface of the cultivation medium after fully absorbing water, and their effect is to keep moisture, but the regulating effect on the moisture of the cultivation medium is limited. The mosses used in the present invention are living mosses, which must first be planted and survived on the trunk, and can only be planted when the white flower Paphiopedilum seedlings can be wrapped in the nutrient soil when they grow to a certain extent, and their effect will have both fixed nutrient soil and white flower Paphiopedilum seedlings, and water conservation in the early stage of cultivation.

[0011] The above-mentioned method for cultivating white flowered Paphiopedilum trunk comprises the following steps:

[0012] (1) Trunk selection

[0013] Choose evergreen trees;

[0014] (2) Planting auxiliary plants

[0015] Plant moss on tree trunks;

[0016] (3) Planting seedlings

[0017] After the moss roots wrap around the tree trunk, plant the Paphiopedilum saplings;

[0018] (4) Water and fertilizer management

[0019] After planting, fix an infusion bag filled with root-promoting liquid above the seedlings, use a syringe to replenish water for the seedlings, and remove the infusion bag after the new roots of the seedlings are naturally fixed on the trunk, and switch to regular spraying.

[0020] In step (1), the evergreen tree is an evergreen tree with a diameter greater than 10 cm and a shade degree of 60-80%.

[0021] The seedlings in step (3) are Paphiopedilum leucoderma tissue culture seedlings that have been hardened and transplanted in a greenhouse for 10-20 months, have 3-5 leaves and good rooting. At this time, the seedlings have short and thin roots that are not easy to break, and are cultivated and survived using trunks.

[0022] Step (3) is performed as follows: a tree trunk with good moss growth is selected, and the moss is cut downward from the top of the tree with a knife (only a small cut is made, and the cut moss is still connected to the moss on the tree trunk). The size of the opening is determined according to the root system of the planted Paphiopedilum leucophyllum, and the root system of the Paphiopedilum leucophyllum seedling is placed in the opening, and the root system is attached to the tree trunk, nutrient soil is placed on the root system, the cut moss is restored, and the moss and the Paphiopedilum leucophyllum seedling are fixed with a cotton rope.

[0023] The nutrient soil is a mixture of peat soil and orchid-specific slow-release fertilizer in a volume ratio of 15-20:1, and the dosage is 10-20g nutrient soil per seedling. The cultivation matrix used in the daily artificial cultivation of white flowered Paphiopedilum is usually peat soil, leaf mold, bark, coconut husk, moss, etc., so that the white flowered Paphiopedilum plant grows slowly, and the mixed slow-release fertilizer of the present invention can provide nutrition for a long time without causing waste, and can provide loose growth matrix and long-term nutrition for white flowered Paphiopedilum, and promote its growth and development.

[0024] In step (4): the root-promoting solution is a mixed solution containing 30-60ppm naphthaleneacetic acid and 10-30ppm indolebutyric acid, and the infusion bag is a commercially available ordinary large tree infusion bag. Naphthaleneacetic acid and indolebutyric acid have the effects of promoting cell elongation, rooting and inducing female flower formation, and are commonly used to promote cuttings or seedlings such as cuttings and transplanting to take root. However, as plant growth regulators, naphthaleneacetic acid and indolebutyric acid, when used in combination, must strictly control the concentration range and mode of action according to specific species, otherwise the effect is not obvious, or damage or even kill the active material. The present invention optimizes the use of naphthaleneacetic acid and indolebutyric acid mixed solution, promotes the rapid rooting of white flower Paphiopedilum seedlings, and improves the survival rate.

[0025] In view of the problems existing in the current artificial cultivation of Paphiopedilum leucophyllum, the inventor has established a method for cultivating Paphiopedilum leucophyllum on a trunk, using mosses as auxiliary plants to cultivate Paphiopedilum leucophyllum on a trunk. The method promotes the rapid rooting and survival of Paphiopedilum leucophyllum seedlings through operations such as trunk selection, planting auxiliary plants, planting seedlings, and water and fertilizer management. The present invention solves the problems of easy breakage of fleshy roots, difficulty in fixing on the trunk, slow root growth of tissue culture seedlings, and low survival rate in the cultivation of Paphiopedilum leucophyllum trunks, and for the first time innovatively uses living mosses as auxiliary plants to achieve successful cultivation of Paphiopedilum leucophyllum on a trunk. This method can make the survival rate of Paphiopedilum tissue culture seedlings trunks cultivated for one year reach 85.5%, which is 1.84 times that of not cultivating moss and 1.40 times that of not using root-promoting solution; the average number of buds and leaves reached 1.67 and 4.2 respectively, which is also higher than 1.0 and 2.5 without cultivating moss and 1.2 and 3.1 without using root-promoting solution.

[0026] In addition, the present invention can not only improve the growth space position of Paphiopedilum leucodendron, increase the ornamental value, and be more conducive to people's appreciation, but also can make full use of a large number of trees in various gardens, and form a symbiotic system of Paphiopedilum leucodendron and trees after successful cultivation, and Paphiopedilum leucodendron can directly use the epidermal moisture of the trunk, reducing management costs. In a word, the present invention is simple to operate and easy to master, has good effect, and is conducive to popularization and application. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 The diagram is a result diagram of cultivating Paphiopedilum leucoderma using the present invention. DETAILED DESCRIPTION

[0028] To clearly illustrate how the present invention is implemented, the following further illustrates the invention through examples. The preparation of the tissue culture seedlings of Paphiopedilum leucoderma: the tissue culture bottle seedlings of Paphiopedilum leucoderma with good rooting are hardened and cleaned, and then transplanted to a mixed substrate of pine bark: volcanic rock = 3:1, and conventional management is performed in a greenhouse. After 10-20 months of cultivation, the healthy seedlings with good growth and 3-5 leaves and developed root systems are selected for trunk cultivation.

[0029] Example 1

[0030] (1) Trunk selection: Select trunks of pine, camphor, or Cyclobalanopsis glauca with a diameter of 10-17 cm and a shade of 60-80%.

[0031] (2) Planting seedlings: When the transplanted Paphiopedilum tissue culture seedlings grow to 12 months old, select strong seedlings that are sturdy, free of pests and diseases, and have 3-5 leaves for trunk cultivation.

[0032] (3) Planting: First, use cotton rope to loosely fix the transplanted seedlings of Paphiopedilum leucophyllum on the trunk, so that the roots are attached to the trunk, then use gauze strips to fix 10-20g of nutrient soil on the roots of the seedlings, and untie the cotton rope. The nutrient soil is a mixture of peat soil and orchid-specific slow-release fertilizer in a volume ratio of 15:1.

[0033] (4) Water and fertilizer management: After planting, fix a large tree infusion bag filled with root-promoting liquid above the seedlings, use a syringe to replenish water for the seedlings, and remove the infusion bag after the new roots of the seedlings are naturally fixed on the tree trunk, and replace it with regular spraying. The root-promoting liquid is a mixture containing 50ppm naphthaleneacetic acid and 20ppm indolebutyric acid.

[0034] Example 2

[0035] (1) Trunk selection: Select trunks of pine, camphor, or Cyclobalanopsis glauca with a diameter of 10-17 cm and a shade of 60-80%.

[0036] (2) Planting auxiliary plants: Planting moss on tree trunks.

[0037] (3) Planting seedlings: When the transplanted Paphiopedilum tissue culture seedlings grow to 12 months old, select strong seedlings with 3-5 leaves that are strong, free of pests and diseases for trunk cultivation. Plant Paphiopedilum seedlings after the moss roots wrap around the trunk. Select a trunk with good moss growth, use a knife to cut the moss from the top of the tree downwards. The size of the opening depends on the root system of the Paphiopedilum to be planted. Place the roots of the Paphiopedilum seedlings into the opening and make the roots stick to the trunk. Put 10-20g of nutrient soil on the roots, restore the cut moss, and then fix the moss and Paphiopedilum seedlings with cotton rope. The nutrient soil is a mixture of peat soil and orchid-specific slow-release fertilizer (source: Israel Chemical Group Orl 318S, the same in the following cases) in a volume ratio of 15:1. (4) Water and fertilizer management: Spray with clean water regularly.

[0038] Example 3

[0039] (1) Trunk selection: Select trunks of pine, camphor, or Cyclobalanopsis glauca with a diameter of 10-17 cm and a shade of 60-80%.

[0040] (2) Planting auxiliary plants: Planting moss on tree trunks.

[0041] (3) Planting seedlings: When the transplanted Paphiopedilum tissue culture seedlings grow to 12 months, select strong seedlings with 3-5 leaves that are strong, free of pests and diseases for trunk cultivation. Plant Paphiopedilum seedlings after the moss roots wrap around the trunk. Select a trunk with good moss growth, use a knife to cut the moss from the top of the tree downwards. The size of the opening depends on the root system of the Paphiopedilum to be planted. Put the root system of the Paphiopedilum seedling into the opening and make the root system stick to the trunk. Put 10-20g of nutrient soil on the root system, restore the cut moss, and then fix the moss and Paphiopedilum seedlings with cotton rope. The nutrient soil is a mixture of peat soil and orchid-specific slow-release fertilizer in a volume ratio of 15:1.

[0042] (4) Water and fertilizer management: After planting, fix a large tree infusion bag filled with root-promoting liquid above the seedlings, use a syringe to replenish water for the seedlings, and remove the infusion bag after the new roots of the seedlings are naturally fixed on the tree trunk, and replace it with regular spraying. The root-promoting liquid is a mixture containing 50ppm naphthaleneacetic acid and 20ppm indolebutyric acid.

[0043] Example 4

[0044] The method is substantially the same as Example 3, except that in step (4), the concentration of naphthylacetic acid is 60 ppm and the concentration of indolebutyric acid is 0 ppm.

[0045] Example 5

[0046] The method is substantially the same as Example 3, except that in step (4), the concentration of naphthaleneacetic acid is 0 ppm and the concentration of indolebutyric acid is 30 ppm.

[0047] Example 3 Survival and growth of Paphiopedilum edulis for 10 months Figure 1 The survival rates and growth conditions of all examples are shown in Table 1.

[0048] Table 1 Comparison of survival and growth of Paphiopedilum leucophyllum in Examples 1 to 5

[0049] Survival rate of seedlings after 1 year of cultivation (%) Average number of buds per plant Average number of leaves per plant Example 1 46.5 1.0 2.5 Example 2 60.9 1.2 3.1 Example 3 85.5 1.67 4.2 Example 4 74 1.5 3.0 Example 5 68.5 1.33 3.5

[0050] By comparison with Table 1, it can be seen that the survival rate and growth of the cultivation method of Example 3 of the present invention are significantly better than those of Examples 1, 2, 4, and 5.

Claims

1. A method for cultivating a white-flowered Paphiopedilum trunk, characterized in that: Use moss as an auxiliary plant to cultivate white-flowered Paphiopedilum on tree trunks.

2. The method for cultivating white flowered Paphiopedilum trunk according to claim 1, characterized in that: Plant moss on the tree trunk before planting white flowered Paphiopedilum seedlings.

3. The method for cultivating white flowered Paphiopedilum trunk according to claim 2, characterized in that: The moss is living moss, and the trunk is an evergreen tree trunk.

4. The method for cultivating white paphiopedilum trunk according to claim 3, characterized in that it comprises the following steps: (1) Trunk selection Choose evergreen trees; (2) Planting auxiliary plants Plant moss on tree trunks; (3) Planting seedlings After the moss roots wrap around the tree trunk, plant the Paphiopedilum saplings; (4) Water and fertilizer management After planting, fix an infusion bag filled with root-promoting liquid above the seedlings, use a syringe to replenish water for the seedlings, and remove the infusion bag after the new roots of the seedlings are naturally fixed on the trunk, and switch to regular spraying.

5. The method for cultivating white paphiopedilum trunk according to claim 4, characterized in that In step (1), the evergreen tree is an evergreen tree with a diameter greater than 10 cm and a shade degree of 60-80%.

6. The method for cultivating white flowered Paphiopedilum trunk according to claim 4, characterized in that The seedlings in step (3) are Paphiopedilum leucophyllum tissue culture seedlings that have been hardened and transplanted in a greenhouse for 10-20 months, have 3-5 leaves and are well rooted.

7. The method for cultivating white paphiopedilum trunk according to claim 6, characterized in that Step (3) is performed as follows: a tree trunk with good moss growth is selected, and the moss is cut from the top of the tree downward with a knife, wherein the size of the opening is determined according to the root system of the planted Paphiopedilum leucophyllum, and the root system of the Paphiopedilum leucophyllum seedling is placed in the opening, and the root system is attached to the tree trunk, and nutrient soil is placed on the root system, and the cut moss is restored, and then the moss and the Paphiopedilum leucophyllum seedling are fixed with a cotton rope.

8. The method for cultivating white-flowered Paphiopedilum trunk according to claim 7, characterized in that: The nutrient soil is a mixture of peat soil and orchid-specific slow-release fertilizer in a volume ratio of 15-20:1, and the dosage is 10-20g of nutrient soil per seedling.

9. The method for cultivating white paphiopedilum trunk according to claim 4, characterized in that In step (4): the root promoting solution is a mixed solution containing 30-60 ppm naphthylacetic acid and 10-30 ppm indolebutyric acid, and the infusion bag is a commercially available ordinary large tree infusion bag.

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