Biological tissue culture device

Abstract

The invention relates to the technical field of biological tissue culture, in particular to a biological tissue culture device which comprises a tissue culture frame, a plurality of tissue culture bottles are placed on the lower surface of the inner side of the tissue culture frame, each tissue culture bottle is composed of a bottle body and a bottle cap, a moving plate is slidably connected to the upper surface of the inner side of the tissue culture frame, and a servo motor is fixedly connected to the lower surface of the moving plate. The output end of the servo motor is fixedly connected with a lead screw, the outer surface of the lead screw is in threaded connection with a lifting plate, the upper surface of the lifting plate is fixedly connected with a driving motor, and the output end of the driving motor is fixedly connected to the upper surface of one rotating piece. When the driving motor rotates, the rotating piece can drive the bottle cap clamp to rotate the caps of the multiple tissue culture bottles at the same time, the time for manually unscrewing the caps one by one is greatly saved, it can be ensured that the rotating speed and time parameters of all the tissue culture bottles are kept consistent, it is ensured that all the caps are evenly unscrewed, and the working efficiency is improved. And the standardization degree of the experiment can be effectively improved.

Description

Technical Field

[0001] The invention relates to the technical field of biological tissue culture, in particular to a biological tissue culture device. Background Art

[0002] As an important part of modern biological science and agricultural technology, biological tissue culture technology plays a key role in many fields such as plant breeding, cell culture, and biomedical production. With the in-depth study of biology, the demand for biological tissue culture devices is also increasing. Biological tissue culture devices simulate and optimize the natural environment of organism growth to achieve the regeneration and expansion of cells, tissues or organs under laboratory conditions. However, the device still has the following defects after specific use:

[0003] When conducting tissue culture on organisms, operators are required to gradually open the lids of tissue culture bottles to open the bottles and harden the seedlings under strong light. However, operators are prone to errors when tightening the lids, resulting in different degrees of looseness of the lids of the tissue culture bottles, thereby affecting the environmental stability in the tissue culture bottles. In addition, when operators unscrew the lids one by one, the unscrewing time for each tissue culture bottle is different, which can easily lead to inconsistent experimental results and fluctuations in production quality.

[0004] Therefore, the present invention proposes a biological tissue culture device to make up for and improve the deficiencies of the prior art. Summary of the invention

[0005] In view of the defects existing in the prior art, the present invention provides a biological tissue culture device which can effectively solve the above technical problems.

[0006] The technical implementation scheme of the present invention is: a biological tissue culture device, including a tissue culture rack, a plurality of tissue culture bottles are placed on the lower surface of the inner side of the tissue culture rack, the tissue culture bottles are composed of a bottle body and a bottle cap, a moving plate is slidably connected to the upper surface of the inner side of the tissue culture rack, a servo motor is fixedly connected to the lower surface of the moving plate, the output end of the servo motor is fixedly connected to a screw rod, the outer surface of the screw rod is threadedly connected to a lifting plate, a plurality of circular holes are equidistantly penetrated through the upper surface of the lifting plate, the outer surface of the lifting plate away from the screw rod is slidably connected to the outer surface of the bottom of the moving plate, the upper surface of the lifting plate is fixedly connected to a driving motor, the output end of the driving motor is fixedly connected to the upper surface of one of the rotating parts, the outer surface of the rotating part is fixedly connected to a bearing, the outer surface of the bearing is fixedly connected to the inside of the circular hole through the upper surface of the lifting plate, the outer surface of the rotating part away from the bearing is transmission-connected to a synchronous belt, the inner side of the synchronous belt away from one end of the rotating part is transmission-connected to another synchronous belt, and the outer surface of the top of each rotating part is transmission-connected to the inner side of the synchronous belt.

[0007] The top of the driving member is connected with the driving member to the driving member, and the upper surface of the driving member is connected with the driving member to the driving member to drive the member to move upwards and downwards to the driving member to drive the member to move upwards and downwards to the driving member to move upwards and downwards to the driving member

[0008] More preferably, the lower surface of the inner side of the tissue culture rack is slidably connected to a plurality of bottle body clamps, the inner sides of the two ends of the bottle body clamps are slidably connected to fixed rods, the upper surface of the fixed rods is fixedly connected to guide members, the outer surface of the guide member is slidably connected to an extrusion block, the two sides of the bottom of the extrusion block are inclined downward, and the upper surface of the extrusion block is extrusion-fitted with the lower surface of the lifting plate.

[0009] More preferably, the outer surface of the bottom of the circular hole through which the lifting plate is provided is annular and fixedly connected to a fixing block, both sides of the fixing block are arc-shaped, the outer surface of the bottom of the rotating member is symmetrically fixedly connected to a guide rod, the outer surface of the guide rod away from the rotating member is slidably connected to a bottle cap clamp, the outer surface of the bottle cap clamp is squeezed into fit with the arcs at both ends of the fixing block, the top of the bottle cap clamp close to the rotating member is fixedly connected to a return spring, the inner wall of the return spring is sleeved on the outer surface of the guide rod, and the end of the return spring away from the guide rod is fixedly connected to the outer surface of the rotating member.

[0010] More preferably, an air storage box is fixedly connected to the upper surface of the lifting plate, an extrusion frame is slidably connected to the inner wall of the air storage box, a return spring is fixedly connected to the lower surface of the front side of the top of the extrusion frame, the lower surface of the return spring is fixedly connected to the upper surface of the air storage box, the lower surface of the rear side of the extrusion frame is extruded and fitted with the trapezoidal protrusion on the upper surface of the raised wheel, a plurality of ventilation pipes are through-connected to the outer surface of the air storage box, and one end of the ventilation pipe away from the air storage box is through-connected to the upper surface of the ventilation ring.

[0011] More preferably, both sides of the bottom of the squeezing block are squeezed with the inner side between the two bottle body clamps, and the inner side between the two bottle body clamps is symmetrically fixedly connected with a pull-back spring.

[0012] More preferably, the groove formed on the inner side of the bottle cap clamp is matched with the protrusion on the outer surface of the top of the tissue culture bottle.

[0013] More preferably, the outer surface of the fixed wheel is connected to a rotating block in an annular shape, and one end of the rotating block away from the fixed wheel is clamped on the inner side of the raised wheel.

[0014] Compared with the prior art, the present invention has the following advantages:

[0015] 1. The present invention can drive the bottle cap clamp to rotate the caps of multiple tissue culture bottles simultaneously through the rotating part when the driving motor rotates, which greatly saves the time of manually unscrewing the caps one by one, and can ensure that the rotation speed and time parameters of each tissue culture bottle are consistent, ensuring that each cap can be unscrewed at the same time, which can effectively improve the standardization of the experiment. Unscrewing at the same time can ensure that the tissue culture bottles are exposed to the same environmental conditions at the same time point, reducing the difference in experimental results caused by the difference in the time of twisting the bottle caps.

[0016] 2. The present invention drives the suction cup to generate negative pressure to adsorb the lids of the tissue culture bottles when the extrusion frame slides upward, so that multiple lids can be automatically moved upward at one time to achieve batch processing. Since the suction cups descend at the same time, it can ensure that each lid is lifted evenly, reducing the inconsistency caused by improper human operation.

[0017] 3. When the lifting plate moves downward, the present invention can prompt the squeezing block to push downward at the same time to clamp the outer surface of the bottom of the tissue culture bottle, so that the tissue culture bottle can be firmly clamped to prevent the bottle body from moving or tipping over during the rotation of the bottle cap, thereby ensuring the stability and safety of the operation. In addition, by fixing the tissue culture bottle, the vibration generated during rotation can be reduced to avoid unnecessary interference or damage to the culture in the tissue culture bottle. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 It is a schematic diagram of the three-dimensional structure of the present invention.

[0019] Figure 2 It is a schematic diagram of the three-dimensional structure of the servo motor, lead screw, tissue culture rack and other components of the present invention.

[0020] Figure 3 It is a schematic diagram of the three-dimensional structure of the tissue culture bottle of the present invention.

[0021] Figure 4 It is a schematic diagram of the three-dimensional structure of components such as the drive motor, synchronous belt and rotating parts of the present invention.

[0022] Figure 5 It is a schematic diagram of the three-dimensional structure of the synchronous belt, bottle cap clamp, fixing block and other components of the present invention.

[0023] Figure 6 It is a schematic diagram of the three-dimensional structure of the lifting plate, rotating part, driving motor and other components of the present invention.

[0024] Figure 7 It is a schematic diagram of the three-dimensional structure of the synchronous belt, bottle cap clamp, bearing and other components of the present invention.

[0025] Figure 8 It is a sectional view of the three-dimensional structure of the guide rod, return spring, bearing and other components of the present invention.

[0026] Fig. 9 It is a schematic diagram of the three-dimensional structure of the cover-opening mechanism of the present invention.

[0027] Fig.10 It is a schematic diagram of the three-dimensional structure of the extrusion frame, return spring, cam wheel and other components of the present invention.

[0028] Fig.11 It is a sectional view of the three-dimensional structure of the components such as the pressing member, the suction cup and the air storage box of the present invention.

[0029] Fig.12 It is a schematic diagram of the three-dimensional structure of the support block, the rotating block and the arc spring mechanism of the present invention.

[0030] Fig.13 It is a schematic diagram of the three-dimensional structure of the fixing mechanism of the present invention.

[0031] Fig.14 It is a schematic diagram of the three-dimensional structure of the fixing rod, the guide member, the return spring and other components of the present invention.

[0032] Fig.15 It is a sectional view of the three-dimensional structure of the bottle body clamp, the extruder and the fixing rod of the present invention.

[0033] The markings of the components in the accompanying drawings are as follows: 1-tissue culture rack, 11-lifting plate, 12-rotating member, 13-tissue culture bottle, 14-servo motor, 15-screw, 16-moving plate, 17-drive motor, 18-synchronous belt, 19-bottle cap fixture, 110-fixed block, 111-guide rod, 112-reset spring, 113-bearing, 2-pressing member, 21-suction cup, 22-gas storage box, 23-extrusion frame, 24-return spring, 25-raised wheel, 26-ventilation pipe, 27-fixed wheel, 28-ventilation ring, 29-support block, 210-pressing spring, 211-rotating block, 212-arc spring, 3-bottle body fixture, 31-extrusion fast, 32-fixing rod, 33-guide member, 34-pull-back spring. DETAILED DESCRIPTION

[0034] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0035] The present invention will be further described below in conjunction with the embodiments.

[0036] Embodiments of the present invention

[0037] refer to Figures 1 to 8As shown, a biological tissue culture device includes a tissue culture rack 1, a plurality of tissue culture bottles 13 are placed on the lower surface of the inner side of the tissue culture rack 1, the tissue culture bottles 13 are composed of a bottle body and a bottle cap, the tissue culture rack 1 is used to place the tissue culture bottles 13, the upper surface of the inner side of the tissue culture rack 1 is slidably connected with a moving plate 16, the lower surface of the moving plate 16 is fixedly connected with a servo motor 14, the moving plate 16 is used to drive the servo motor 14 to slide on the upper surface of the inner side of the tissue culture rack 1, the output end of the servo motor 14 is fixedly connected with a screw rod 15, the servo motor 14 is used to drive the screw rod 15 to rotate simultaneously, the outer surface of the screw rod 15 is threadedly connected with a lifting plate 11, the screw rod 15 is used to drive the lifting plate 11 to move up and down on the inner side of the tissue culture rack 1, and the upper surface of the lifting plate 11 is equidistantly penetrated A plurality of circular holes are provided, and the outer surface of the lifting plate 11 away from the screw rod 15 is slidably connected to the outer surface of the bottom of the moving plate 16. The upper surface of the lifting plate 11 is fixedly connected to a driving motor 17, and the output end of the driving motor 17 is fixedly connected to the upper surface of one of the rotating parts 12. The driving motor 17 is used to drive the rotating part 12 to rotate. The outer surface of the rotating part 12 is fixedly connected to a bearing 113, and the outer surface of the bearing 113 is fixedly connected to the inside of the circular hole provided through the upper surface of the lifting plate 11. The bearing 113 is used to cause the rotating part 12 to rotate inside. The outer surface of the rotating part 12 away from the bearing 113 is transmission-connected with a synchronous belt 18, and the inner side of the synchronous belt 18 away from one end of the rotating part 12 is transmission-connected to Another synchronous belt 18, the outer surface of the top of each rotating member 12 is transmission-connected to the inner side of the synchronous belt 18, the synchronous belt 18 is used to drive multiple rotating members 12 to rotate simultaneously, the outer surface of the bottom of the circular hole through the lifting plate 11 is annular and fixedly connected to a fixed block 110, both sides of the fixed block 110 are arc-shaped, the outer surface of the bottom of the rotating member 12 is symmetrically fixedly connected to a guide rod 111, the outer surface of the guide rod 111 away from the rotating member 12 is slidably connected to a bottle cap clamp 19, the guide rod 111 is used to limit the bottle cap clamp 19 from sliding on the outer surface of the guide rod 111, the outer surface of the bottle cap clamp 19 is squeezed and matched with the arcs at both ends of the fixed block 110, and the arcs at both ends of the fixed block 110 can The bottle cap clamp 19 is driven to be squeezed inwardly. A return spring 112 is fixedly connected to the top of the bottle cap clamp 19 close to the rotating member 12. The inner wall of the return spring 112 is sleeved on the outer surface of the guide rod 111. The end of the return spring 112 away from the guide rod 111 is fixedly connected to the outer surface of the rotating member 12. The return spring 112 is used to drive the bottle cap clamp 19 to reset and move. When the driving motor 17 rotates, the bottle cap clamp 19 can be driven to be squeezed inwardly through the rotating member 12 to drive the lids of multiple tissue culture bottles 13 to rotate at the same time, which greatly saves the time of manually unscrewing the lids one by one, and can ensure that the rotation speed and time parameters of each tissue culture bottle 13 are consistent, ensuring that each lid is unscrewed evenly.

[0038] refer to Figures 9 to 12As shown, a biological tissue culture device, the inner side of the bottom of the rotating member 12 is slidably connected with a pressing member 2, the upper surface of the inner side of the pressing member 2 is fixedly connected with a pressing spring 210, the upper surface of the pressing spring 210 is fixedly connected to the lower surface of the inner side of the rotating member 12, the pressing spring 210 is used to drive the pressing member 2 to reset, the lower surface of the pressing member 2 is symmetrically fixedly connected with a suction cup 21, the outer surface of the top of the suction cup 21 is connected to the center of the inner side of the rotating member 12 through sliding, the suction cup 21 is used to adsorb the upper surface of the tissue culture bottle 13, the outer surface of the top of the rotating member 12 is connected to the ventilation ring 28 through, the outer surface of the output shaft of the driving motor 17 is fixedly connected A fixed wheel 27 is connected, and the outer surface of the fixed wheel 27 is annularly rotatably connected to a rotating block 211. The fixed wheel 27 is used to drive the convex wheel 25 to rotate in one direction. The side of the rotating block 211 close to the fixed wheel 27 is fixedly connected to an arc spring 212, and the side of the arc spring 212 away from the rotating block 211 is fixedly connected to the outer surface of the fixed wheel 27. The arc spring 212 is used to drive the rotating block 211 to reset. The end of the rotating block 211 away from the fixed wheel 27 is clamped with the convex wheel 25, and the end of the rotating block 211 away from the fixed wheel 27 is clamped on the inner side of the convex wheel 25. The upper surface of the convex wheel 25 is fixedly connected with a trapezoidal convex block. The inner side of the bottom of the wheel 25 is slidably connected with a support block 29, and the lower surface of the support block 29 is fixedly connected to the upper surface of the lifting plate 11. The support block 29 is used to limit the rotation of the raised wheel 25 on the upper surface of the support block 29. The upper surface of the lifting plate 11 is fixedly connected with an air storage box 22, and the inner wall of the air storage box 22 is slidably connected with an extrusion frame 23. The extrusion frame 23 is used to generate negative pressure on the inner side of the air storage box 22. The lower surface of the front side of the top of the extrusion frame 23 is fixedly connected with a return spring 24, and the lower surface of the return spring 24 is fixedly connected to the upper surface of the air storage box 22. The return spring 24 is used to reset the extrusion frame 23, and the lower surface of the rear side of the extrusion frame 23 is fixedly connected to the convex The trapezoidal protrusions on the upper surface of the lifting wheel 25 are squeezed and fitted, and the trapezoidal protrusions on the upper surface of the raised wheel 25 can drive the extrusion frame 23 to move upward when rotating. The outer surface of the gas storage box 22 is connected with multiple ventilation pipes 26. The end of the ventilation pipe 26 away from the gas storage box 22 is connected to the upper surface of the ventilation ring 28. The ventilation pipe 26 is used to transport gas to the interior of the gas storage box 22. When the extrusion frame 23 slides upward, it drives the suction cup 21 to generate negative pressure to adsorb the lid of the tissue culture bottle 13, so that multiple lids can be automatically moved upward at one time to realize batch processing. Since the suction cup 21 descends at the same time, it can ensure that each lid is lifted evenly.

[0039] refer to Figures 13 to 15As shown, a biological tissue culture device is shown, wherein a plurality of bottle body clamps 3 are slidably connected to the lower surface of the inner side of a tissue culture rack 1, and the bottle body clamps 3 are used to squeeze the outer surface of the bottom of a tissue culture bottle 13, and the inner sides of both ends of the bottle body clamps 3 are slidably connected to fixed rods 32, and the fixed rods 32 are used to make the bottle body clamps 3 slide on the outer surface of the fixed rods 32, and the upper surface of the fixed rods 32 is fixedly connected to a guide member 33, and the outer surface of the guide member 33 is slidably connected to an extrusion block 31, and the guide member 33 is used to make the extrusion block 31 slide on the upper surface of the guide member 33, and the two sides of the bottom of the extrusion block 31 are inclined downward, and the upper surface of the extrusion block 31 is The surface is extruded and matched with the lower surface of the lifting plate 11, and the two sides of the bottom of the extrusion fastener 31 are extruded and configured with the inner side between the two bottle body clamps 3. The inner sides between the two bottle body clamps 3 are symmetrically fixedly connected with a pull-back spring 34. The pull-back spring 34 is used to drive the two bottle body clamps 3 to reset. When the lifting plate 11 moves downward, the extrusion fastener 31 can be pushed downward at the same time to clamp the outer surface of the bottom of the tissue culture bottle 13, so that the tissue culture bottle 13 can be firmly clamped to prevent the tissue culture bottle 13 from moving or tipping over during the rotation of the top of the tissue culture bottle 13, thereby ensuring the stability and safety of the operation.

[0040] The complete working principle and steps of the above embodiment are as follows:

[0041] refer to Figures 1 to 8 As shown, when the biological culture device is in the initial state, the lifting plate 11 is slidably connected to the top of the outer surface of the screw rod 15, the servo motor 14 and the drive motor 17 are both in the off state, the bottle cap clamp 19 has not yet been attached to the two ends of the fixing block 110, and the return spring 112 is in a naturally relaxed state;

[0042] When the operator uses the device to culture organisms, he first places the tissue culture bottle 13 containing the organisms on the upper surface of the inner side of the tissue culture rack 1, thereby starting to open the bottle and harden the organisms in the tissue culture bottle 13 under strong light. When the top of the tissue culture bottle 13 needs to be rotated, the operator pushes the moving plate 16 slidably connected to the inner side of the tissue culture rack 1 to a suitable position, thereby starting the servo motor 14. As the servo motor 14 starts, it drives the screw rod 15 fixedly connected to the output end of the servo motor 14 to rotate at the same time, and when the screw rod 15 rotates, it drives the lifting plate 11 threadedly connected to the outer surface of the screw rod 15 to slide downward, and the screw rod 1 The circular hole through which the upper surface of the lifting plate 11 is opened is fixedly connected to the outer surface of the bearing 113, and the inner wall of the bearing 113 is rotatably connected to the outer surface of the top of the rotating member 12. As the lifting plate 11 moves downward, the bearing 113 and the rotating member 12 are driven to move downward at the same time. As the lower surface of the rotating member 12 contacts the top of the tissue culture bottle 13, the driving motor 17 fixedly connected to the upper surface of the lifting plate 11 can be started. As the output shaft of the driving motor 17 rotates, one of the rotating members 12 is driven to rotate. The outer surface of the top of the rotating member 12 is transmission-connected to the inner side of the synchronous belt 18, and when one of the rotating members 12 rotates, it will drive the rotating member 12 to rotate. The entire rotating member 12 is driven to rotate simultaneously, and the rotating member 12 rotates, and the guide rod 111 symmetrically fixedly connected to the outer surface of the bottom of the rotating member 12 is driven to rotate simultaneously. As the guide rod 111 rotates, the bottle cap clamp 19 slidably connected to the outer surface of the guide rod 111 is driven to rotate. As the bottle cap clamp 19 rotates ninety degrees simultaneously with the rotating member 12, the outer surface of the bottle cap clamp 19 contacts the arc surface of the outer surface of the fixed block 110, and the bottle cap clamp 19 is squeezed inwardly. When the bottle cap clamp 19 is squeezed inwardly, the return spring 112 fixedly connected to the inner side of the top of the bottle cap clamp 19 is moved to the extrusion position. state, as the bottle cap clamp 19 is squeezed inward, the inner side of the bottle cap clamp 19 will fit the outer surface of the top of the tissue culture bottle 13, and at this time, when the rotating member 12 rotates again, it will drive the outer surface of the top of the tissue culture bottle 13 to rotate at the same time, which greatly saves the time of manually unscrewing the caps one by one, and can ensure that the rotation speed and time parameters of each tissue culture bottle 13 are consistent, ensuring that each cap can be unscrewed at the same time, which can effectively improve the standardization of the experiment. At the same time, unscrewing can ensure that the tissue culture bottle 13 is exposed to the same environmental conditions at the same time point, reducing the difference in experimental results caused by the difference in the time of screwing the bottle cap;

[0043] As the rotation angle of the lifting plate 11 increases, the outer surface of the bottle cap clamp 19 will be separated from the inner side of the fixing block 110. At this time, the return spring 112 in a compressed state will drive the bottle cap clamp 19 to slide outward on the outer surface of the guide rod 111. As the bottle cap clamp 19 slides outward, the inner side of the bottle cap clamp 19 will be separated from the outer surface of the top of the tissue culture bottle 13, thereby starting the servo motor 14, causing the servo motor 14 to drive the screw rod 15 to rotate in the opposite direction, prompting the screw rod 15 to drive the lifting plate 11 to move upward for reset.

[0044] refer to Figures 9 to 12 As shown, when the biological culture device is in the initial state, the outer surface of the bottom of the extrusion frame 23 is slidably connected to the bottom of the inner side of the gas storage box 22, the arc spring 212 and the return spring 24 are in a natural relaxation state, and the end of the rotating block 211 away from the fixed wheel 27 is clamped on the inner side of the raised wheel 25;

[0045] When the lifting plate 11 drives the rotating member 12 to move downward and contact the top of the tissue culture bottle 13, the pressing member 2 slidably connected to the inner side of the bottom of the rotating member 12 will shrink toward the inner side of the rotating member 12, and the pressing member 2 will drive the suction cup 21 fixedly connected to the bottom of the pressing member 2 to slide inward at the same time. As the pressing member 2 slides toward the inner side of the rotating member 12, the pressing spring 210 fixedly connected to the upper surface of the pressing member 2 will be prompted to move to a compressed state. At this time, the lower surface of the suction cup 21 is attached to the top of the tissue culture bottle 13, and the driving motor 17 drives the rotating member 12 to rotate clockwise, while driving the fixed wheel 27 fixedly connected to the outer surface of the output end of the driving motor 17 to rotate clockwise at the same time, and the fixed wheel 27 will drive the circular rotating The rotating block 211 connected to the outer surface of the fixed wheel 27 rotates simultaneously. Since the side of the rotating block 211 close to the fixed wheel 27 is fixedly connected to the outer side of the arc spring 212, the end of the rotating block 211 away from the fixed wheel 27 can always be clamped on the inner wall of the convex wheel 25, and the rotating block 211 will not drive the convex wheel 25 to rotate simultaneously when it rotates counterclockwise. When the top of the tissue culture bottle 13 is completely unscrewed and needs to be removed, the drive motor 17 is started at this time to drive the drive motor 17 to rotate counterclockwise. As the fixed wheel 27 rotates counterclockwise, the rotating block 211 on the outer surface will be driven to rotate simultaneously, and when the rotating block 211 rotates, it will be clamped on the inner side of the convex wheel 25 and drive the convex wheel 25 to rotate simultaneously, and the convex wheel 25 rotates counterclockwise. When the outer surface of the extrusion frame 23 is connected to the outer surface of the air storage box 22, the air pipe 26 is connected to the upper surface of the air ring 28, and the inner side of the air ring 28 is connected to the center of the rotating part 12, and the inner wall of the center of the rotating part 12 is connected to the top of the suction cup 21. When the outer surface of the bottom of the extrusion frame 23 slides upward inside the air storage box 22, the gas will enter the rotating part 12 due to the bottom of the suction cup 21. At the center of the rotating part 12, since the inner side of the gas storage box 22 is in a negative pressure state, the gas at the center of the rotating part 12 will enter the inner side of the ventilation ring 28, and the gas of the ventilation ring 28 will enter the inner side of the gas storage box 22 from the inner side of the ventilation tube 26. When the bottom of the suction cup 21 is attached to the upper surface of the tissue culture bottle 13, the suction cup 21 can adsorb the top of the tissue culture bottle 13 when it sucks air inward. The servo motor 14 rotates in the opposite direction to drive the upper surface of the tissue culture bottle 13 to move upward through the rotating part 12, so that multiple lids can be automatically moved upward at one time to realize batch processing. Since the suction cup 21 descends at the same time, it can ensure that each lid is lifted evenly, reducing the inconsistency caused by improper human operation.

[0046] When the lid on the top of the tissue culture bottle 13 needs to be removed, the drive motor 17 is started to rotate counterclockwise to drive the fixed wheel 27 to rotate counterclockwise, and the fixed wheel 27 drives the raised wheel 25 to rotate counterclockwise at the same time through the rotating block 211. As the fixed wheel 27 rotates counterclockwise, the trapezoidal protrusion on the upper surface of the raised wheel 25 will be separated from the extrusion frame 23. At this time, the return spring 24 in the stretched state will drive the extrusion frame 23 to slide downward, and the outer surface of the bottom of the extrusion frame 23 will slide downward on the inner wall of the gas storage box 22, causing the negative pressure state inside the gas storage box 22 to disappear. At this time, the adsorption force at the bottom of the suction cup 21 will also disappear, so that the lid on the top of the tissue culture bottle 13 can be separated from the bottom of the suction cup 21.

[0047] refer to Figures 13 to 15 As shown in the figure, as the lifting plate 11 moves downward, the lower surface of the lifting plate 11 will touch the top of the extrusion block 31 and push the extrusion block 31 downward. Since the middle part of the lower surface of the extrusion block 31 is slidably connected to the outer surface of the guide member 33, as the extrusion block 31 moves downward, it will slide downward on the outer side of the guide member 33, and when the extrusion block 31 slides downward, the two sides of the bottom of the extrusion block 31 will squeeze between the two bottle body clamps 3, causing the two bottle body clamps 3 to slide to the side away from the extrusion block 31. As the bottle body clamps 3 move away from the extrusion block 31, When one side slides, the pull-back spring 34 fixedly connected between the two bottle body clamps 3 will be moved to a stretched state, and the inner side of the bottom of the bottle body clamp 3 will be clamped to the outer surface of the bottom of the tissue culture bottle 13, so that the tissue culture bottle 13 can be firmly clamped to prevent the outer surface of the tissue culture bottle 13 from moving or tipping over during the rotation of the top of the tissue culture bottle 13, thereby ensuring the stability and safety of the operation, and by fixing the tissue culture bottle 13, the vibration generated during the rotation can be reduced, avoiding unnecessary interference or damage to the culture in the tissue culture bottle 13;

[0048] When the top of the tissue culture bottle 13 is completely rotated, the screw rod 15 will drive the lifting plate 11 to move upward. At this time, the pull-back spring 34 in the stretched state will drive the two bottle body clamps 3 to move toward the side close to the squeezing block 31. During the movement, the two bottle body clamps 3 will fit on both sides of the bottom of the squeezing block 31 and drive the squeezing block 31 to slide upward and return to the initial state.

[0049] Although the present disclosure has been shown and described with reference to specific exemplary embodiments of the present disclosure, it should be understood by those skilled in the art that various changes in form and details may be made to the present disclosure without departing from the spirit and scope of the present disclosure as defined by the appended claims and their equivalents. Therefore, the scope of the present disclosure should not be limited to the above-mentioned embodiments, but should be determined not only by the appended claims, but also by the equivalents of the appended claims.

Claims

1. A biological tissue culture device, comprising a tissue culture rack (1), a plurality of tissue culture bottles (13) are placed on the lower surface of the inner side of the tissue culture rack (1), the tissue culture bottles (13) are composed of a bottle body and a bottle cap, a moving plate (16) is slidably connected to the upper surface of the inner side of the tissue culture rack (1), a servo motor (14) is fixedly connected to the lower surface of the moving plate (16), a screw rod (15) is fixedly connected to the output end of the servo motor (14), a lifting plate (11) is threadedly connected to the outer surface of the screw rod (15), a plurality of circular holes are equidistantly formed on the upper surface of the lifting plate (11), and the outer surface of the lifting plate (11) away from the screw rod (15) is slidably connected to the outer surface of the bottom of the moving plate (16), wherein: The upper surface of the lifting plate (11) is fixedly connected to a driving motor (17), the output end of the driving motor (17) is fixedly connected to the upper surface of one of the rotating members (12), the outer surface of each rotating member (12) is fixedly connected to a bearing (113), the outer surface of the bearing (113) is fixedly connected to the inside of a circular hole through which the upper surface of the lifting plate (11) is provided, the outer surface of the rotating member (12) away from the bearing (113) is transmission-connected to a synchronous belt (18), the inner side of the synchronous belt (18) away from one end of the rotating member (12) is transmission-connected to another synchronous belt (18), and the outer surface of the top of each rotating member (12) is transmission-connected to the inner side of the synchronous belt (18).

2. A biological tissue culture device according to claim 1, characterized in that: The inner side of the bottom of the rotating member (12) is slidably connected to a pressing member (2); the upper surface of the inner side of the pressing member (2) is fixedly connected to a pressing spring (210); the upper surface of the pressing spring (210) is fixedly connected to the lower surface of the inner side of the rotating member (12); the lower surface of the pressing member (2) is symmetrically fixedly connected to a suction cup (21); the outer surface of the top of the suction cup (21) is slidably connected to the center of the inner side of the rotating member (12); the outer surface of the top of the rotating member (12) is connected to a ventilation ring (28); the outer surface of the output shaft of the driving motor (17) is fixedly connected to a fixed wheel (27); the fixed The outer surface of the wheel (27) is rotatably connected to a rotating block (211); a side of the rotating block (211) close to the fixed wheel (27) is fixedly connected to an arc spring (212); a side of the arc spring (212) away from the rotating block (211) is fixedly connected to the outer surface of the fixed wheel (27); an end of the rotating block (211) away from the fixed wheel (27) is clamped with a raised wheel (25); a trapezoidal raised block is fixedly connected to the upper surface of the raised wheel (25); a support block (29) is slidably connected to the inner side of the bottom of the raised wheel (25); and a lower surface of the support block (29) is fixedly connected to the upper surface of the lifting plate (11).

3. A biological tissue culture device according to claim 2, characterized in that: The lower surface of the inner side of the tissue culture rack (1) is slidably connected to a plurality of bottle body clamps (3), the inner sides of both ends of the bottle body clamps (3) are slidably connected to fixing rods (32), the upper surface of the fixing rods (32) is fixedly connected to a guide member (33), the outer surface of the guide member (33) is slidably connected to an extrusion block (31), the two sides of the bottom of the extrusion block (31) are inclined downward, and the upper surface of the extrusion block (31) is extrusion-matched with the lower surface of the lifting plate (11).

4. A biological tissue culture device according to claim 3, characterized in that: The outer surface of the bottom of the circular hole through which the lifting plate (11) is formed is annular and fixedly connected to a fixing block (110); both sides of the fixing block (110) are arc-shaped; the outer surface of the bottom of the rotating member (12) is symmetrically fixedly connected to a guide rod (111); the outer surface of the guide rod (111) at a side away from the rotating member (12) is slidably connected to a bottle cap clamp (19); the outer surface of the bottle cap clamp (19) is extruded and matched with the arcs at both ends of the fixing block (110); the top of the bottle cap clamp (19) at a side close to the rotating member (12) is fixedly connected to a return spring (112); the inner wall of the return spring (112) is sleeved on the outer surface of the guide rod (111); and one end of the return spring (112) away from the guide rod (111) is fixedly connected to the outer surface of the rotating member (12).

5. A biological tissue culture device according to claim 4, characterized in that: The upper surface of the lifting plate (11) is fixedly connected to an air storage box (22), the inner wall of the air storage box (22) is slidably connected to an extrusion frame (23), the lower surface of the front side of the top of the extrusion frame (23) is fixedly connected to a return spring (24), the lower surface of the return spring (24) is fixedly connected to the upper surface of the air storage box (22), the lower surface of the rear side of the extrusion frame (23) is extruded and matched with the trapezoidal protrusion on the upper surface of the raised wheel (25), the outer surface of the air storage box (22) is connected to a plurality of ventilation pipes (26), and one end of the ventilation pipe (26) away from the air storage box (22) is connected to the upper surface of the ventilation ring (28).

6. A biological tissue culture device according to claim 5, characterized in that: The two sides of the bottom of the squeezing block (31) are squeezed with the inner side between the two bottle body clamps (3), and the inner side between the two bottle body clamps (3) is symmetrically fixedly connected with a pull-back spring (34).

7. A biological tissue culture device according to claim 6, characterized in that: The groove formed on the inner side of the bottle cap clamp (19) is matched with the protrusion on the outer surface of the top of the tissue culture bottle (13).

8. A biological tissue culture device according to claim 7, characterized in that: The outer surface of the fixed wheel (27) is connected to a rotating block (211) in a circular shape and rotationally connected thereto. One end of the rotating block (211) away from the fixed wheel (27) is clamped on the inner side of the raised wheel (25).

Images