Breeding method of cotton aphidius gifuensis

Abstract

The invention discloses a method for breeding aphidius gifuensis, which adopts a specific breeding process, adopts specific male parent and female parent domestication with staged humidity and temperature and adopts different subculture schemes to construct a specific aphidius gifuensis breeding method, and aims to solve the problems that the existing aphidius gifuensis is easy to cause hyperparasitism when the number of cotton aphids is low, and the breeding efficiency is low. The cotton aphid is massively propagated, and the prevention and treatment effect is poor. According to the technical scheme, the breeding mode that the female parents and the male parents are alternately subjected to low-temperature domestication has a remarkable influence on the parasitism rate of F5-generation individuals obtained through breeding, compared with a control group, the parasitism rate difference of the aphidius gifuensis is remarkable, and the survival rate in low-temperature feeding and high-temperature feeding is also remarkably influenced; the stress resistance of the aphidius gifuensis can be improved, and the green and sustainable development of the cotton industry can be realized.

Description

Technical Field

[0001] The invention belongs to the field of animal production application, and specifically relates to the technical field of breeding of cotton aphid wasps. Background Art

[0002] With the long-term and large-scale use of chemical pesticides, parasitic wasps, as a special group of Hymenoptera, can specifically and efficiently control agricultural and forestry pests, and their role in pest control has attracted more and more attention. Parasitic wasps are of various species and large numbers. They are a special type of symbionts that can parasitize on the surface or body of insects, feed on the surface of the host or feed on the tissues of the host. Studies have reported that parasitic wasps are important parasitic natural enemies of cotton aphids in cotton fields in North China, and the field parasitism rate can reach 80%. Aphids parasitized by parasitic wasps do not stop feeding and die immediately, but can continue to feed and grow until the parasitic wasps in the body develop into mature larvae. However, inside the parasitized aphids, parasitic wasps regulate and transform the physiology, metabolism, biosynthesis, and immunity of aphids, making the internal environment of aphids suitable for the growth and development of parasitic wasps. Cotton aphids can be parasitized by a variety of parasitic wasps, among which the cotton aphid braconid wasp is the dominant cotton aphid parasitic wasp in northern cotton fields, which can effectively suppress the population of cotton aphids in early summer. The cotton aphid wasp belongs to the family Aphididae of the superfamily Ichneumonoidea of ​​the order Hymenoptera. It is distributed in China, Japan, Indonesia, and South Korea. The cotton aphid wasp parasitizes aphids and is quite selective in its host. The individual development of the cotton aphid wasp goes through four stages: egg, larva, pupa, and adult. The mated female wasps produce offspring of both sexes, while the unmated females reproduce parthenogenetically.

[0003] The control of cotton aphids by the cotton aphid wasp is not only reflected in direct parasitism, but the cotton aphid wasp can also change the growth, development and reproduction of pests through coercion. The cotton aphid wasp has a certain effect on reducing the base number of cotton aphids and controlling their field populations, and plays an extremely important role in the green control of aphids. When the number of aphids is insufficient, the cotton aphid wasp spends more time searching for hosts. In order to ensure that all eggs are laid in a short period of time, the aphid wasp may choose aphids that have been parasitized as hosts. Regardless of whether super-parasitism occurs, each aphid can only produce one bee, resulting in massive reproduction of aphids and poor control effects. Therefore, breeding cotton aphid strains with strong stress resistance and high parasitism rate is of great significance for the green control of cotton aphid diseases. Summary of the invention

[0004] The purpose of the present invention is to solve the technical problem that the existing cotton aphid wasp is prone to heavy parasitism when the number of cotton aphids is low, resulting in massive reproduction of cotton aphids and poor control effect. It aims to propose a breeding method for cotton aphid wasp. The breeding method of alternating low-temperature acclimation of female and male parents provided in the technical scheme of the present application is used to breed cotton aphid strains with strong stress resistance and high parasitism rate, which has a significant effect on the parasitism rate of the F5 generation individuals obtained by breeding. Compared with the control group, the parasitism rate of the cotton aphid wasp is significantly different, and it also has a significant effect on the survival rate in low-temperature and high-temperature feeding. It can improve the stress resistance of the cotton aphid wasp, which is conducive to the green and sustainable development of the cotton industry.

[0005] To achieve this technical purpose, the present invention adopts the following technical solution.

[0006] The present invention provides a method for breeding aphid wasps, which is carried out by the following method: (1) Before cotton is harvested, healthy natural aphids parasitizing on cotton stalks and leaves are collected from the field and grafted onto cotton seedlings that have been grown in the aphid greenhouse at 21°C-29°C and 65%-75% humidity to allow the aphids to develop and reproduce into virus-free seed aphids for later use; (2) Before cotton is harvested, parasitic aphids parasitized on cotton stalks and leaves by natural aphid wasps are collected from the field, and the aphids are grafted onto the leaves of cotton plants that have been bred with aphids in the breeding bee greenhouse obtained in step (1), at 21°C-29°C and 20%-40% humidity, so that the aphid wasps on the parasitic aphids develop and emerge into virus-free breeding bees for later use; (3) hybridizing the male bees domesticated in step (2) with the female bees to obtain the F1 generation of cotton aphid braconids through breeding; (4) Select and breed the F1 generation of cotton aphid wasps with a strong body. After culturing the male bees at 10℃-18℃ and 20%-40% humidity for 5 days, the temperature is gradually increased to 25℃ and 20%-40% humidity for 10 days. The female bees are then cultured at 21℃-29℃ and 20%-40% humidity for 15 days. The female bees then mate with the male bees to obtain the F2 generation of cotton aphid wasps through breeding. (5) Select and breed the F2 generation of cotton aphid wasps with a strong body. After culturing the female bees at 10℃-18℃ and 20%-40% humidity for 5 days, the temperature was gradually increased to 25℃ and 20%-40% humidity for 10 days. The male bees were cultured at 21℃-29℃ and 20%-40% humidity for 15 days. The female bees then mate with the male bees to obtain the cotton aphid wasps F3 through breeding; (6) Select and breed the robust F3 generation of Aphididae, culture the male bees at 10-18°C and 20%-40% humidity for 5 days, then gradually increase the temperature to 25°C and culture the female bees at 21-29°C and 20%-40% humidity for 15 days. The female bees then mate with the male bees to obtain the F4 generation of Aphididae through breeding; (7) Select and breed the F4 generation of cotton aphid wasps with strong bodies. After culturing the female bees at 10℃-18℃ and 20%-40% humidity for 5 days, the temperature was gradually increased to 25℃ and 20%-40% humidity for 10 days. The male bees were cultured at 21℃-29℃ and 20%-40% humidity for 15 days. The female bees then mate with the male bees, and the stable genetic F5 cotton aphid wasps are obtained through breeding.

[0007] Preferably, in the method for breeding aphid wasps provided by the present invention, the ratio of bee to aphid in step (2) is 1:100.

[0008] Preferably, in the method for breeding aphid wasps provided by the present invention, in the step (4), a robust F1 generation of aphid wasps is bred, the drones are cultured at 14°C and 30% humidity for 5 days, the temperature is gradually increased to 25°C and the humidity is 30% for 10 days, and the females are cultured at 25°C and 30% humidity for 15 days.

[0009] Preferably, in the method for breeding aphid wasps provided by the present invention, in the step (5), a robust F2 generation of aphid wasps is bred, the female wasps are cultured at 14°C and 30% humidity for 5 days, the temperature is gradually increased to 25°C and 30% humidity for 10 days, and the drones are cultured at 25°C and 30% humidity for 15 days.

[0010] Preferably, in the method for breeding aphid wasps provided by the present invention, in the step (6), a robust F3 generation of aphid wasps is bred, and the drones are cultured at 14°C and 30% humidity for 5 days, and then the temperature is gradually increased to 25°C and 30% humidity for 10 days, and the females are cultured at 25°C and 30% humidity for 15 days.

[0011] Preferably, in the method for breeding aphid wasps provided by the present invention, in the step (7), a robust F4 generation of aphid wasps is selected, and the female wasps are cultured at 14°C and 30% humidity for 5 days, and then the temperature is gradually increased to 25°C and 30% humidity for 10 days, and the male wasps are cultured at 25°C and 30% humidity for 15 days.

[0012] Preferably, in the breeding method of the cotton aphid wasp provided by the present invention, 11% honey water is used for feeding throughout the breeding process.

[0013] Furthermore, the present application provides the application of the above-mentioned method for breeding aphid wasps in breeding cotton aphids.

[0014] Furthermore, the present application provides the application of the above-mentioned method for breeding cotton aphid wasps in the prevention and control of cotton aphids.

[0015] By implementing the technical solution of the present invention, the following beneficial effects can be achieved: (1) The present invention provides a method for breeding a cotton aphid wasp, which adopts a specific breeding process, adopts specific male and female domestication with staged humidity and temperature, and constructs a specific breeding method for a cotton aphid wasp. This method can effectively solve the technical problem that the existing cotton aphid wasp is prone to heavy parasitism when the number of cotton aphids is low, resulting in a large number of cotton aphids and poor control effect.

[0016] (2) The breeding method of alternating low-temperature acclimation of female and male parents provided in the technical solution of the present application has a significant effect on the parasitism rate of the F5 generation individuals obtained through breeding. The parasitism rate of the cotton aphid wasp bred using the technical solution provided in the present application is 74%-95%, which is significantly different from the parasitism rate of the control group of 51%-69%. It also has a significant effect on the survival rate in low-temperature and high-temperature breeding, with a survival rate of 24%-53%, which is significantly higher than 8%-19% of the control group. This can enhance the stress resistance of the cotton aphid wasp and is conducive to the green and sustainable development of the cotton industry. DETAILED DESCRIPTION The technical solution of the present invention is clearly and completely described below in conjunction with the embodiments. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0018] In this example, cotton aphids were collected from cotton fields at the Korla Experimental Base of the Institute of Plant Protection, Xinjiang Academy of Agricultural Sciences (Korla City, Heshilike Township, Bayinguoleng Mongol Autonomous Prefecture, Xinjiang Uygur Autonomous Region, 41.75ºN, 85.81ºE). Cotton aphid braconids were collected from aphids on licorice and camel thorn around the Korla Experimental Base of the Institute of Plant Protection, Xinjiang Academy of Agricultural Sciences, and were used in the experiment after they emerged in the laboratory.

[0019] The relevant experiments were carried out in the cotton fields of the Korla Experimental Base of the Institute of Plant Protection of the Xinjiang Academy of Agricultural Sciences. The cotton variety used was Xinluzhong 67, and 1 film 6 rows were sown with wide and narrow rows, with a sowing width of 200 cm, a film spacing of 42 cm, a row spacing of 13+62+13, and a plant spacing of 10 cm. No pesticides were sprayed during the experiment.

[0020] The experimental methods used in the following examples are conventional methods unless otherwise specified. The materials, reagents, etc. used in the following examples are all commercially available unless otherwise specified.

[0021] Embodiment 1: A method for breeding aphid wasps The present invention provides a method for breeding aphid wasps, which is carried out by the following method: (1) Before cotton is harvested, healthy natural aphids parasitizing on cotton stalks and leaves are collected from the field and grafted onto cotton seedlings that have been grown in the aphid greenhouse at 21°C-29°C and 65%-75% humidity to allow the aphids to develop and reproduce into virus-free seed aphids for later use; (2) Before cotton is harvested, parasitic aphids parasitized on cotton stalks and leaves by natural aphid wasps are collected from the field, and the aphids are grafted onto the leaves of cotton plants that have been bred with aphids in the breeding bee greenhouse obtained in step (1), at 21°C-29°C and 20%-40% humidity, so that the aphid wasps on the parasitic aphids develop and emerge into virus-free breeding bees for later use; (3) hybridizing the male bees domesticated in step (2) with the female bees to obtain the F1 generation of cotton aphid braconids through breeding; (4) Select and breed the F1 generation of cotton aphid wasps with a strong body. After culturing the male bees at 10℃-18℃ and 20%-40% humidity for 5 days, the temperature is gradually increased to 25℃ and 20%-40% humidity for 10 days. The female bees are then cultured at 21℃-29℃ and 20%-40% humidity for 15 days. The female bees then mate with the male bees to obtain the F2 generation of cotton aphid wasps through breeding. (5) Select and breed the F2 generation of cotton aphid wasps with a strong body. After culturing the female bees at 10℃-18℃ and 20%-40% humidity for 5 days, the temperature was gradually increased to 25℃ and 20%-40% humidity for 10 days. The male bees were cultured at 21℃-29℃ and 20%-40% humidity for 15 days. The female bees then mate with the male bees to obtain the cotton aphid wasps F3 through breeding; (6) Select and breed the robust F3 generation of Aphididae, culture the male bees at 10-18°C and 20%-40% humidity for 5 days, then gradually increase the temperature to 25°C and culture the female bees at 21-29°C and 20%-40% humidity for 15 days. The female bees then mate with the male bees to obtain the F4 generation of Aphididae through breeding; (7) Select and breed the F4 generation of cotton aphid wasps with strong bodies. After culturing the female bees at 10℃-18℃ and 20%-40% humidity for 5 days, the temperature was gradually increased to 25℃ and 20%-40% humidity for 10 days. The male bees were cultured at 21℃-29℃ and 20%-40% humidity for 15 days. The female bees then mate with the male bees, and the stable genetic F5 cotton aphid wasps are obtained through breeding.

[0022] Embodiment 2: A method for breeding aphid wasps A method for breeding cotton aphid wasps is carried out by the following method: before cotton is harvested, healthy natural original aphids parasitizing on cotton stalks and cotton leaves are collected from the field, and the aphids are grafted onto cotton seedlings that have been cultivated in an aphid greenhouse, at 21°C and 65% humidity, so that the original aphids can develop and reproduce into virus-free seed aphids for later use; before cotton is harvested, parasitic aphids parasitized by natural original aphid wasps are collected from the field, and the aphid wasps are grafted onto the leaves of cotton plants that have been bred with aphids in the obtained bee greenhouse, at 21°C and 20% humidity, so that the aphid wasps on the parasitic aphids can develop and emerge into virus-free seed bees for later use; domesticated male bees are hybridized with female bees to obtain the F1 generation of cotton aphid wasps through breeding; the F1 generation of cotton aphid wasps with healthy body shape are bred, and the male bees are hybridized with female bees to obtain the F1 generation of cotton aphid wasps through breeding, and the F1 generation of cotton aphid wasps with healthy body shape is selected and the male bees are hybridized with female bees to obtain the F1 generation of cotton aphid wasps; ... After culturing at 0℃ and 20% humidity for 5 days, the temperature was gradually increased to 25℃, and the humidity was 20% for 10 days. The female bees were cultured at 21℃ and 20% humidity for 15 days. The female bees then mated with the male bees to obtain the F2 generation of cotton aphid bracon wasps through breeding; the F2 generation of cotton aphid bracon wasps with strong body were selected, and the female bees were cultured at 10℃ and 20% humidity for 5 days, and the temperature was gradually increased to 25℃, and the humidity was 20% for 10 days. The male bees were cultured at 21℃ and 20% humidity for 15 days. The female bees then mated with the male bees to obtain the F3 generation of cotton aphid bracon wasps through breeding; the F3 generation of cotton aphid bracon wasps with strong body were selected, and the culture method was the same as the F2 generation to obtain the F4 generation of cotton aphid bracon wasps; the F4 generation of cotton aphid bracon wasps with strong body were selected, and the culture method was the same as the F3 generation to obtain the F5 generation of cotton aphid bracon wasps with stable genetics through breeding. 11% honey water was used for feeding throughout the breeding process.

[0023] Embodiment 3: A method for breeding Cotton Aphididae A method for breeding cotton aphid wasps is carried out by the following method: before cotton is harvested, healthy natural original aphids parasitizing on cotton stalks and cotton leaves are collected from the field, and the aphids are grafted onto cotton seedlings that have been cultivated in an aphid greenhouse, at 23°C and 67% humidity, so that the original aphids can develop and reproduce into virus-free seed aphids for later use; before cotton is harvested, parasitic aphids parasitized by natural original aphid wasps are collected from the field, and the aphid wasps are grafted onto the leaves of cotton plants that have been bred with aphids in the obtained bee greenhouse, at 23°C and 25% humidity, so that the aphid wasps on the parasitic aphids can develop and emerge into virus-free seed bees for later use; domesticated male bees are hybridized with female bees to obtain the F1 generation of cotton aphid wasps through breeding; the F1 generation of cotton aphid wasps with healthy body shape are bred, and the male bees are hybridized with female bees to obtain the F1 generation of cotton aphid wasps through breeding, and the F1 generation of cotton aphid wasps with healthy body shape is selected and the male bees are hybridized with female bees to obtain the F1 generation of cotton aphid wasps; ... After culturing at 2℃ and 25% humidity for 5 days, the temperature was gradually increased to 25℃, and the humidity was 25% for 10 days. The female bees were cultured at 23℃ and 25% humidity for 15 days. The female bees then mated with the male bees to obtain the F2 generation of cotton aphid bracon wasps through breeding; the F2 generation of cotton aphid bracon wasps with strong body shape were selected, and the female bees were cultured at 12℃ and 25% humidity for 5 days, and the temperature was gradually increased to 25℃, and the humidity was 25% for 10 days. The male bees were cultured at 23℃ and 25% humidity for 15 days. The female bees then mated with the male bees to obtain the F3 generation of cotton aphid bracon wasps through breeding; the F3 generation of cotton aphid bracon wasps with strong body shape were selected, and the culture method was the same as the F2 generation to obtain the F4 generation of cotton aphid bracon wasps; the F4 generation of cotton aphid bracon wasps with strong body shape were selected, and the culture method was the same as the F3 generation to obtain the F5 generation of cotton aphid bracon wasps with stable genetics through breeding. 11% honey water was used for feeding throughout the breeding process.

[0024] Embodiment 4: A method for breeding aphid wasps A method for breeding cotton aphid wasps is carried out by the following method: before cotton is harvested, healthy natural original aphids parasitizing on cotton stalks and cotton leaves are collected from the field, and the aphids are grafted onto cotton seedlings that have been cultivated in an aphid greenhouse, at 25°C and 69% humidity, so that the original aphids can develop and reproduce into virus-free seed aphids for later use; before cotton is harvested, parasitic aphids parasitized by natural original aphid wasps are collected from the field, and the aphid wasps are grafted onto the leaves of cotton plants that have been bred with aphids in the obtained bee greenhouse, at 25°C and 30% humidity, so that the aphid wasps on the parasitic aphids can develop and emerge into virus-free seed bees for later use; domesticated male bees are hybridized with female bees to obtain the F1 generation of cotton aphid wasps through breeding; the F1 generation of cotton aphid wasps with healthy body shape are bred, and the male bees are hybridized with female bees to obtain the F1 generation of cotton aphid wasps through breeding; the F1 generation of cotton aphid wasps with healthy body shape are selected, and the male bees are hybridized with female bees to obtain the F1 generation of cotton aphid wasps ... After culturing at 4℃ and 30% humidity for 5 days, the temperature was gradually increased to 25℃ and the humidity was 30% for 10 days. The female bees were cultured at 25℃ and 30% humidity for 15 days. The female bees then mated with the male bees to obtain the F2 generation of cotton aphid bracon wasps through breeding; the F2 generation of cotton aphid bracon wasps with strong body were selected and bred. After the female bees were cultured at 14℃ and 30% humidity for 5 days, the temperature was gradually increased to 25℃ and the humidity was 30% for 10 days. The male bees were cultured at 25℃ and 30% humidity for 15 days. The female bees then mated with the male bees to obtain the F3 generation of cotton aphid bracon wasps through breeding; the F3 generation of cotton aphid bracon wasps with strong body were selected and bred. The culture method was the same as the F2 generation to obtain the F4 generation of cotton aphid bracon wasps; the F4 generation of cotton aphid bracon wasps with strong body were selected and bred. The culture method was the same as the F3 generation. The stable genetic cotton aphid bracon wasps F5 were obtained through breeding. 11% honey water was used for feeding throughout the breeding process.

[0025] Embodiment 5: A method for breeding aphid wasps A method for breeding cotton aphid wasps is carried out by the following method: before cotton is harvested, healthy natural original aphids parasitizing on cotton stalks and cotton leaves are collected from the field, and the aphids are grafted onto cotton seedlings that have been cultivated in an aphid greenhouse, at 27°C and 71% humidity, so that the original aphids can develop and reproduce into virus-free seed aphids for later use; before cotton is harvested, parasitic aphids parasitized by natural original aphid wasps are collected from the field, and the aphid wasps are grafted onto the leaves of cotton plants that have been bred with aphids in the obtained bee greenhouse, at 27°C and 35% humidity, so that the aphid wasps on the parasitic aphids can develop and emerge into virus-free seed bees for later use; domesticated male bees are hybridized with female bees to obtain the F1 generation of cotton aphid wasps through breeding; the F1 generation of cotton aphid wasps with healthy body shape are bred, and the male bees are hybridized with female bees to obtain the F1 generation of cotton aphid wasps through breeding, and the F1 generation of cotton aphid wasps with healthy body shape is selected and the male bees are hybridized with female bees to obtain the F1 generation of cotton aphid wasps; ...; the F1 generation of cotton aphid wasps with healthy body shape is selected and the male bees are hybridized with female bees; the F1 generation of cotton aphid wasps with healthy body shape is selected and the male bees After culturing at 6℃ and 35% humidity for 5 days, the temperature was gradually increased to 25℃ and the humidity was 35% for 10 days. The female bees were cultured at 27℃ and 35% humidity for 15 days. The female bees then mated with the male bees to obtain the F2 generation of cotton aphid bracone through breeding; the F2 generation of cotton aphid bracone with strong body shape was selected and bred. After the female bees were cultured at 16℃ and 35% humidity for 5 days, the temperature was gradually increased to 25℃ and the humidity was 35% for 10 days. The male bees were cultured at 27℃ and 35% humidity for 15 days. The female bees then mated with the male bees to obtain the F3 generation of cotton aphid bracone through breeding; the F3 generation of cotton aphid bracone with strong body shape was selected and bred. The culture method was the same as the F2 generation to obtain the F4 generation of cotton aphid bracone; the F4 generation of cotton aphid bracone with strong body shape was selected and bred. The culture method was the same as the F3 generation. The F5 of cotton aphid bracone with stable genetics was obtained through breeding. 11% honey water was used for feeding throughout the breeding process.

[0026] Embodiment 6: A breeding method of Cotton Aphid Braconidae A method for breeding cotton aphid wasps is carried out by the following method: before cotton is harvested, healthy natural original aphids parasitizing on cotton stalks and cotton leaves are collected from the field, and the aphids are grafted onto cotton seedlings that have been cultivated in an aphid greenhouse, at 29°C and 75% humidity, so that the original aphids can develop and reproduce into virus-free seed aphids for later use; before cotton is harvested, parasitic aphids parasitized by natural original aphid wasps are collected from the field, and the aphid wasps are grafted onto the leaves of cotton plants that have been bred with aphids in the obtained bee greenhouse, at 29°C and 40% humidity, so that the aphid wasps on the parasitic aphids can develop and emerge into virus-free seed bees for later use; domesticated male bees are hybridized with female bees to obtain the F1 generation of cotton aphid wasps through breeding; the F1 generation of cotton aphid wasps with healthy body shape are bred, and the male bees are hybridized with female bees to obtain the F1 generation of cotton aphid wasps through breeding, and the F1 generation of cotton aphid wasps with healthy body shape is selected and the male bees are hybridized with female bees to obtain the F1 generation of cotton aphid wasps; ... After culturing at 8℃ and 40% humidity for 5 days, the temperature was gradually increased to 25℃ and the humidity was 40% for 10 days. The female bees were cultured at 29℃ and 40% humidity for 15 days. The female bees then mated with the male bees to obtain the F2 generation of cotton aphid bracon wasps through breeding; the F2 generation of cotton aphid bracon wasps with strong body shape were selected, and the female bees were cultured at 18℃ and 40% humidity for 5 days, and the temperature was gradually increased to 25℃ and the humidity was 40% for 10 days. The male bees were cultured at 29℃ and 40% humidity for 15 days. The female bees then mated with the male bees to obtain the F3 generation of cotton aphid bracon wasps through breeding; the F3 generation of cotton aphid bracon wasps with strong body shape were selected, and the culture method was the same as the F2 generation to obtain the F4 generation of cotton aphid bracon wasps; the F4 generation of cotton aphid bracon wasps with strong body shape were selected, and the culture method was the same as the F3 generation to obtain the F5 generation of cotton aphid bracon wasps with stable genetics through breeding. 11% honey water was used for feeding throughout the breeding process.

[0027] Example 7: Comparison of the breeding effects of Aphididae Based on the breeding method of cotton aphid bracone recorded in the above-mentioned Examples 2 to 6, the characteristics of the obtained F5 generation related to cotton aphid bracone were measured, and set as test group 1, test group 2, test group 3, test group 4, and test group 5; and the F1 generation breeding method in the breeding method of cotton aphid bracone recorded in Examples 2 to 6 was used to breed to the F5 generation control group 1, control group 2, control group 3, control group 4, and control group 5. 30 single-headed female bees and drones were selected and small cotton balls were stuffed into their finger-shaped tubes. 11% honey water was dripped on the cotton with a disposable plastic dropper for group feeding. After feeding for 24 hours, they were paired for 2 hours at a ratio of female: male = 1:1. 100 2-3 year old cotton aphids were selected and placed in a small insect cage, and each pair of mating cotton aphid braconids was inoculated, and then placed in an artificial climate box (temperature 25°C, relative humidity 80%, photoperiod 14L:10D) for breeding. After the aphids were formed, the aphids were removed with a brush and placed in a 2 ml finger tube, the number was counted, and the parasitism rate was calculated. The parasitism rate was calculated by the following method:

[0028] Each treatment in the experimental group and the control group was repeated 3 times, and the data were recorded and sorted using Microsoft Excel 2010 software. SPSS 21.0 software was used for significance analysis, with a confidence probability of 0.95. P <0.05 was considered significant difference.

[0029] Table 1: Comparison of breeding results of Cotton Aphid Wasp

[0030] According to the relevant results measured in the above Table 1, the parasitism rate of the cotton aphid braconid wasp in the test groups 1 to 5 provided in Examples 2 to 6 provided in the present application is significantly higher than the parasitism rate of the cotton aphid braconid wasp in the control group 1 to control group 5. It can be seen that the breeding method of alternating low-temperature acclimation of female and male parents provided in the present application has a significant effect on the parasitism rate of the F5 generation individuals obtained by breeding. The parasitism rate of the cotton aphid braconid wasp in the test groups 1 to 5 provided in Examples 2 to 6 of the present application shows a trend of first increasing and then decreasing, and there are significant differences between the groups. Among them, the parasitism rate of the test group 3 provided in Example 4 is the highest. In the process of acclimation and breeding of the cotton aphid braconid wasp, with the increase of temperature and humidity, the parasitism rate of the obtained offspring continues to rise, but it is not the case that the higher the temperature and humidity, the stronger the parasitic ability of the obtained offspring.

[0031] Example 8: Comparison of the breeding effects of Aphididae Based on the breeding method of cotton aphid bracone recorded in the above-mentioned Examples 2 to 6, the characteristics of the obtained F5 generation-related cotton aphid bracone were measured, and set as test group 1, test group 2, test group 3, test group 4, and test group 5; and the F1 generation breeding method in the breeding method of cotton aphid bracone recorded in Examples 2 to 6 was used to breed to the F5 generation control group 1, control group 2, control group 3, control group 4, and control group 5. 100 cotton aphid bracone were randomly selected from each group and placed in a small insect cage. Each group was set to be raised at 5°C and 40°C for 24h, and each treatment of the test group and the control group was repeated 3 times, and the survival rate was counted.

[0032] Table 2: Comparison of stress resistance of Cotton Aphid Wasp

[0033] According to the relevant results measured in the above Table 2, the stress resistance of the cotton aphid braconid wasps in the test groups 1 to 5 provided in Examples 2 to 6 provided in the present application is significantly higher than that of the cotton aphid braconid wasps in the control groups 1 to 5. It can be seen that the breeding method of alternating low-temperature acclimation of female and male parents provided in the present application has a significant effect on the stress resistance of the F5 generation individuals obtained by breeding. The survival rate of the cotton aphid braconid wasps in the test groups 1 to 5 provided in Examples 2 to 6 of the present application showed a trend of first increasing and then decreasing, among which the differences between the groups were significant, among which the survival rate of the test group 3 provided in Example 4 was the highest. During the acclimation and breeding of the cotton aphid braconid wasps, with the increase of temperature and humidity, the survival rate of the obtained offspring in low or high temperatures continued to rise, but it was not the case that the higher the temperature and humidity, the stronger the stress resistance of the obtained offspring.

[0034] The embodiments described above are only some embodiments of the present invention, not all embodiments. The detailed description of the embodiments of the present invention is not intended to limit the scope of the invention claimed for protection, but only represents selected embodiments of the present invention. All other embodiments obtained without creative work and related deductions and substitutions made by ordinary technicians in the field under the conditions of the concept of the present invention belong to the scope of protection of the present invention.

Claims

1. A method for breeding Cotton Aphid Braconidae, characterized in that: The following method is used: (1) Before cotton is harvested, healthy natural aphids parasitizing on cotton stalks and leaves are collected from the field and grafted onto cotton seedlings that have been grown in the greenhouse at 21℃-29℃ and 65%-75% humidity to allow the original aphids to develop and reproduce into virus-free seed aphids for future use; (2) Before cotton is harvested, parasitic aphids parasitized by natural aphid wasps on cotton stems and leaves are collected from the field, and the aphids are grafted onto the leaves of cotton plants that have been bred with aphids in the greenhouse obtained in step (1), at 21°C-29°C and 20%-40% humidity, so that the aphid wasps on the parasitic aphids develop and emerge into virus-free seed bees for use; (3) hybridizing the male bees domesticated in step (2) with the female bees to obtain the F1 generation of cotton aphid braconids through breeding; (4) Select and breed the F1 generation of cotton aphid wasps with a strong body. After culturing the male bees at 10℃-18℃ and 20%-40% humidity for 5 days, the temperature is gradually increased to 25℃ and 20%-40% humidity for 10 days. The female bees are then cultured at 21℃-29℃ and 20%-40% humidity for 15 days. The female bees then mate with the male bees to obtain the F2 generation of cotton aphid wasps through breeding. (5) Select and breed the F2 generation of cotton aphid wasps with a strong body. After culturing the female bees at 10℃-18℃ and 20%-40% humidity for 5 days, the temperature was gradually increased to 25℃ and 20%-40% humidity for 10 days. The male bees were cultured at 21℃-29℃ and 20%-40% humidity for 15 days. The female bees then mate with the male bees to obtain the cotton aphid wasps F3 through breeding; (6) Select and breed the robust F3 generation of Aphididae, culture the male bees at 10-18°C and 20%-40% humidity for 5 days, then gradually increase the temperature to 25°C and culture the female bees at 21-29°C and 20%-40% humidity for 15 days. The female bees then mate with the male bees to obtain the F4 generation of Aphididae through breeding; (7) Select and breed the F4 generation of cotton aphid wasps with strong bodies. After culturing the female bees at 10℃-18℃ and 20%-40% humidity for 5 days, the temperature was gradually increased to 25℃ and 20%-40% humidity for 10 days. The male bees were cultured at 21℃-29℃ and 20%-40% humidity for 15 days. The female bees then mate with the male bees, and the stable genetic F5 cotton aphid wasps are obtained through breeding.

2. The method for breeding aphid wasps according to claim 1, characterized in that: The ratio of bee to aphid in step (2) is 1:

100.

3. The method for breeding aphid wasps according to claim 1, characterized in that: In the step (4), the F1 generation of cotton aphid wasps with a robust body are selected and cultured. After the drones are cultured at 14°C and 30% humidity for 5 days, the temperature is gradually increased to 25°C and 30% humidity for 10 days, and the females are cultured at 25°C and 30% humidity for 15 days.

4. The method for breeding aphid wasps according to claim 1, characterized in that: In the step (5), the F1 generation of cotton aphid braconids with a robust body are selected, and the drones are cultured at 14°C and 30% humidity for 5 days, and then the temperature is gradually increased to 25°C and 30% humidity for 10 days, and the females are cultured at 25°C and 30% humidity for 15 days.

5. The method for breeding aphid wasps according to claim 1, characterized in that: In the step (5), the F1 generation of cotton aphid braconids with a robust body are selected, and the drones are cultured at 14°C and 30% humidity for 5 days, and then the temperature is gradually increased to 25°C and 30% humidity for 10 days, and the females are cultured at 25°C and 30% humidity for 15 days.

6. The method for breeding Aphididae as claimed in claim 1, characterized in that: In the step (5), the F1 generation of cotton aphid braconids with a robust body are selected, and the drones are cultured at 14°C and 30% humidity for 5 days, and then the temperature is gradually increased to 25°C and 30% humidity for 10 days, and the females are cultured at 25°C and 30% humidity for 15 days.

7. The method for breeding Aphididae as claimed in claim 1, characterized in that: The whole feeding process was fed with 11% honey water.

8. Use of the breeding method of aphid wasps according to any one of claims 1 to 7 in breeding cotton aphids.

9. Use of the breeding method of Aphididae as claimed in any one of claims 1 to 7 in the control of cotton aphids.

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