Natural product lime essential oil and silver nitrate composition and application thereof

Abstract

The invention belongs to the technical field of bacteriostatic and deodorant products, and particularly discloses a composition of natural products of lime essential oil and silver nitrate. Independent use of the high-concentration lime essential oil has an inhibition effect on the escherichia coli BW 25113, but a single AgNO3 group has an unobvious inhibition effect on the escherichia coli BW 25113, the natural product lime essential oil is combined with silver nitrate, and it is found that the sterilization effect on the escherichia coli BW 25113 is obvious, so that the sterilization effect on the escherichia coli BW 25113 is obvious, and the sterilization effect on the escherichia coli BW 25113 is not obvious. The synergistic effect of the lime essential oil and the silver nitrate can reduce the use of the high-concentration lime essential oil, and meanwhile, the sterilization effect is guaranteed. The composition disclosed by the invention can be used for replacing antibiotics while playing a role in inhibiting bacteria more efficiently. The action effect is also applicable to inhibition of staphylococcus aureus and drug-resistant escherichia coli. Besides, the deodorization effect of ammonia water shows that the deodorization effect is enhanced through the combination of lime essential oil and silver nitrate, the usage amount of the two components is low, safety and effectiveness are achieved, raw materials are easy to obtain, and good application prospects are achieved in preparation of antibacterial and deodorization products.

Description

Technical Field

[0001] The invention belongs to the technical field of antibacterial and anti-odor products, and specifically discloses a composition of natural product lime essential oil and silver nitrate and application thereof. Background Art

[0002] Lime essential oil is distilled from lime cones. It has a strong smell, bitter and sweet, and is light yellow. The main components of lime essential oil are terpenes, coriander alcohol, terpineol, etc. It has anti-scorbutic, antibacterial, and antiviral effects.

[0003] Some studies have shown that due to the different mechanisms of antibacterial effects of different essential oil components, the combined use of multiple essential oil components can produce synergistic effects, or antagonistic effects may occur. The development of composite preparations of essential oils combined with other types has become a major research direction. Silver ions can strongly attract the sulfhydryl (-SH) on the protease in bacteria, quickly combine with it, and make the protease inactive, leading to bacterial death. There are not many studies on the synergistic effect of the combined bactericidal effect of essential oils and compounds, so the study of compounds that can have a combined bactericidal effect with lime essential oil has a good application prospect.

[0004] In addition, environments that require disinfection, such as animal husbandry, factories, sewage treatment, landfills, hospitals, etc., also face the need for deodorization. However, existing chemical deodorants have high costs and large usage amounts, and they pose great environmental risks. Therefore, the development of green, low-risk, and low-cost deodorants also has strong practical significance. Summary of the invention

[0005] The purpose of the present invention is to overcome the defects and shortcomings of the prior art and provide a combination of natural product lime essential oil and silver nitrate.

[0006] The second object of the present invention is to provide application of the above combination in the field of antibacterial and deodorization.

[0007] The purpose of this application is achieved through the following technical solutions:

[0008] The invention discloses an antibacterial and deodorizing composition, which consists of lime essential oil and silver nitrate.

[0009] Preferably, in the essential oil complex, the concentration of lime essential oil is 1%-5%, and the concentration of silver nitrate is 0.25-0.5 μg / mL.

[0010] The present invention also provides application of the antibacterial and deodorizing composition in preparing antibacterial and deodorizing products.

[0011] Preferably, the antibacterial target of the product is food-borne bacteria, such as Escherichia coli and / or Staphylococcus aureus.

[0012] More preferably, the Escherichia coli is drug-resistant Escherichia coli.

[0013] Preferably, the deodorizing object of the product is ammonia gas or ammonia water.

[0014] Preferably, the product is in the form of a solid, liquid, pump spray or semisolid gel.

[0015] The present application also provides an antibacterial and deodorizing product, including the antibacterial and deodorizing composition.

[0016] Preferably, the solvent of the antibacterial and deodorizing product is water.

[0017] More preferably, the concentration of the lime essential oil is 1% to 5%. The above deodorant product is preferably in the form of a solution, which can be directly sprayed and is convenient to use.

[0018] Compared with the prior art, the present invention has the following beneficial effects:

[0019] The present invention combines the natural product lime essential oil with silver nitrate, and finds that the combination of lime essential oil and silver nitrate has a significant bactericidal effect on Escherichia coli BW 25113. The synergy of lime essential oil and silver nitrate can reduce the use of high-concentration lime essential oil while ensuring the bactericidal effect. The above-mentioned effect is also applicable to inhibiting Staphylococcus aureus and drug-resistant Escherichia coli. The composition of the present invention has a more efficient antibacterial effect and can replace the use of antibiotics. The deodorizing effect on ammonia water shows that the combination of lime essential oil and silver nitrate enhances the deodorizing effect, the two components are used in low amounts, are safe and effective, and the raw materials are easily available, and have good application prospects in the preparation of antibacterial and deodorizing products. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 The statistical result diagram of the antibacterial effect in Example 2;

[0021] Figure 2 The statistical result diagram of the antibacterial effect in Example 3;

[0022] Figure 3 This is a statistical result diagram of the antibacterial effect in Example 4. DETAILED DESCRIPTION

[0023] The specific embodiments of the present invention below will provide a detailed and comprehensive description of the technical solutions of the present invention. It should be noted that the embodiments provided represent only a part of the present invention, not all of it. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0024] Unless otherwise specified, the test methods used in the examples of the present invention are all conventional methods; the materials, reagents, etc. used are reagents and materials that can be obtained from commercial channels unless otherwise specified, and the commercially available raw materials are used after removing impurities such as insoluble matter by a general filtration method.

[0025] The lime essential oil (LM) used was a product of Guangzhou Wuyuan Essential Oil Co., Ltd.; the silver nitrate (AgNO3) was a McLean brand, MW169.87.

[0026] Judgment principle: If the bacterial count in the experimental group is reduced by more than 0.5 logarithmic values ​​on the basis of the decrease in the bacterial count in the treatment with lime essential oil alone and silver nitrate alone, it indicates that lime essential oil and silver nitrate have a synergistic antibacterial effect.

[0027] Example 1 MIC determination of lime essential oil and silver nitrate against Escherichia coli BW 25113 and Staphylococcus aureus ATCC 29213

[0028] 1. Experimental materials:

[0029] (1) Test: Cool the autoclaved MH broth for later use.

[0030] (2) Lime essential oil: Guangzhou Wuyuan Essential Oil Co., Ltd.; Silver nitrate: McLean brand, MW169.87.

[0031] Test strains: Escherichia coli wild strain BW 25113; Staphylococcus aureus quality control strain ATCC 29213.

[0032] 2. Preparation before the test:

[0033] (1) The wild-type Escherichia coli strain BW 25113 and the quality control strain of Staphylococcus aureus ATCC 29213 were subcultured on agar medium and cultured to an appropriate size.

[0034] (2) Use a precision balance to weigh 0.1 g of silver nitrate powder in a centrifuge tube, add 10 mL of sterile water to dissolve it, and the concentration of silver nitrate is 10 mg / mL. Vortex mix and filter. Pass the filtrate through a 0.22 μm diameter filter membrane for physical sterilization, divide it into sterilized centrifuge tubes, and store it in a -20°C freezer.

[0035] 3. MIC determination experiment:

[0036] (1) Inoculate Escherichia coli BW 25113 and Staphylococcus aureus ATCC 29213 in a centrifuge tube containing 4 mL of MH broth, place in a shaker at 37°C and shake at 180 rpm for 4 h, then remove the centrifuge tube;

[0037] (2) Use MH broth to dilute the incubated bacteria to 100 times, about 106 CFU / mL, reserve;

[0038] (3) Take a sterile 96-well plate, add 180 μL of MH broth medium to well 1, and add 100 μL of MH broth medium to wells 2-12;

[0039] (4) Add 20 μL of lime essential oil or 20 μL of silver nitrate (640 μg / mL) to the first well, pipette and mix well, then pipette 100 μL into the second well, and so on, pipette 100 μL into the tenth well and discard;

[0040] (5) Add 100 μL of diluted bacterial solution to wells 1 to 11 and 200 μL of MH broth to well 12;

[0041] (6) The final concentrations (%) of lime essential oil in wells 1-10 of a 96-well plate were 5%, 2.5%, 1.25%, 0.625%, 0.3125%, 0.156%, 0.078%, 0.039%, 0.0195%, and 0.00975%, well 11 was a growth control, and well 12 was a blank control;

[0042] (7) The final concentrations of silver nitrate (μg / mL) in wells 1-10 of a 96-well plate were 32, 16, 8, 4, 2, 1, 0.5, 0.25, 0.125, and 0.0625, well 11 was a growth control, and well 12 was a blank control;

[0043] (8) Set up 3 replicates for each drug;

[0044] (9) Place the inoculated 96-well plate in a 37°C incubator and incubate for 16-18 hours, then read the results.

[0045] The results are shown in Table 1. The MIC value of BW 25113 lime essential oil was >5%, and the MIC value of silver nitrate was 2 μg / mL; the MIC value of ATCC29213 lime essential oil was >5%, and the MIC value of silver nitrate was 2 μg / mL.

[0046] Table 1 MIC of lime essential oil and silver nitrate against Escherichia coli and Staphylococcus aureus

[0047]

[0048] Example 2 Evaluation of the combined bactericidal effect of lime essential oil and silver nitrate on Escherichia coli BW 25113

[0049] 1. Experimental materials:

[0050] (1) Experiment: Cool the autoclaved LB broth, PBS buffer, and ultrapure water for later use.

[0051] (2) Lime essential oil: Guangzhou Wuyuan Essential Oil Co., Ltd.; Silver nitrate: McLean brand, MW169.87

[0052] Test strain: Escherichia coli wild strain BW 25113 (laboratory collection).

[0053] 2. Preparation before the test:

[0054] (1) The wild-type Escherichia coli strain BW 25113 was subcultured on agar medium and cultured to an appropriate size.

[0055] (2) Use a precision balance to weigh 0.1 g of silver nitrate powder in a centrifuge tube, add 10 mL of sterile water to dissolve it, and the concentration of silver nitrate is 10 mg / mL. Vortex mix and filter. Pass the filtrate through a 0.22 μm diameter filter membrane for physical sterilization, divide it into sterilized centrifuge tubes, and store it in a -20°C freezer.

[0056] 3. Sterilization experiment:

[0057] (1) Inoculate a single colony of Escherichia coli BW 25113 in a centrifuge tube containing 20 mL of LB broth, place the tube in a shaker at 37°C and shake at 180 rpm for 3 h, then remove the tube.

[0058] (2) Centrifuge the cultured bacterial solution at 5500 rpm for 3 min, resuspend in an equal volume of PBS buffer and dilute 10-fold. 7 CFU / mL, for future use.

[0059] (3) This experiment uses a 1 mL system and adds the bacterial solution in (2):

[0060] A) Control group: 0.7 mL PBS + 0.2 mL PBS + 0.1 mL bacterial solution;

[0061] B) Single drug group: 0.7 mL PBS + 0.1 mL A / B drug + 0.1 mL PBS + 0.1 mL bacterial solution;

[0062] C) Combined group: 0.7 mL bacterial solution + 0.1 mL A drug + 0.1 mL B drug + 0.1 mL bacterial solution.

[0063] (4) This test set up 9 groups:

[0064] Escherichia coli BW25113: ①Control group; ②Lime essential oil group: 1 / 2MIC (2.5%) lime essential oil; ③Lime essential oil group: 1 / 4MIC (1.25%) lime essential oil; ④Silver nitrate group: 0.25μg / mL silver nitrate; ⑤Silver nitrate group: 0.5μg / mL silver nitrate; ⑥Combination group: 1 / 4MIC lime essential oil + 0.25μg / mL silver nitrate; ⑦Combination group: 1 / 4MIC lime essential oil + 0.5μg / mL silver nitrate; ⑧Combination group: 1 / 2MIC lime essential oil + 0.25μg / mL silver nitrate; ⑨Combination group: 1 / 2MIC lime essential oil + 0.5μg / mL silver nitrate;

[0065] (5) After the system is prepared, incubate the culture at 37°C with shaking at 180 rpm for 1 h.

[0066] (6) At 1 h, 100 μL of bacterial solution was aspirated from each tube and added to a 2 ml centrifuge tube containing 900 μL PBS buffer for ten-fold gradient dilution. After dilution, 25 μL was dropped on LB agar medium and incubated in a 37°C incubator for 16 to 18 h. The colonies were counted and the experimental results were statistically analyzed after three biological replicates.

[0067] The results are shown in Table 2 and Figure 1 As shown, the bacteria in the blank control group grew normally, indicating that Escherichia coli BW 25113 could grow normally under the experimental conditions; in the single-drug group, there was no significant decrease in Escherichia coli BW 25113 compared with the AgNO3 group alone, indicating that the inhibitory effect of the AgNO3 group alone on Escherichia coli BW 25113 was not obvious; the same results also showed that the use of high-concentration lime essential oil alone had a significant inhibitory effect on Escherichia coli BW 25113; the results of the combined group showed that the synergistic effect of lime essential oil and silver nitrate had a significant bactericidal effect on Escherichia coli BW 25113.

[0068] Table 2 Bactericidal effect of lime essential oil and silver nitrate on wild strain of Escherichia coli BW 25113

[0069]

[0070] Example 3 Evaluation of the combined bactericidal effect of lime essential oil and silver nitrate on Staphylococcus aureus ATCC 29213

[0071] 1. Experimental materials:

[0072] (1) Experiment: Cool the autoclaved LB broth, PBS buffer, and ultrapure water for later use.

[0073] (2) Lime essential oil: Guangzhou Wuyuan Essential Oil Co., Ltd.; Silver nitrate: McLean brand, MW169.87

[0074] Test strain: Staphylococcus aureus quality control strain ATCC 29213 (laboratory collection).

[0075] 2. Preparation before the test:

[0076] (1) Subculture the Staphylococcus aureus quality control strain ATCC 29213 on agar medium and grow to an appropriate size.

[0077] (2) Use a precision balance to weigh 0.1 g of silver nitrate powder in a centrifuge tube, add 10 mL of sterile water to dissolve it, and the concentration of silver nitrate is 10 mg / mL. Vortex mix and filter. Pass the filtrate through a 0.22 μm diameter filter membrane for physical sterilization, divide it into sterilized centrifuge tubes, and store it in a -20°C freezer.

[0078] 3. Sterilization experiment:

[0079] (1) Inoculate a single colony of Staphylococcus aureus ATCC 29213 in a centrifuge tube containing 20 mL of LB broth, place the tube in a shaker at 37°C and shake at 180 rpm for 3 h, then remove the tube.

[0080] (2) Centrifuge the cultured bacterial solution at 5500 rpm for 3 min, resuspend in an equal volume of PBS buffer and dilute 10-fold. 7 CFU / mL, for future use.

[0081] (3) This experiment uses a 1 mL system and adds the bacterial solution in (2):

[0082] A) Control group: 0.7 mL PBS + 0.2 mL PBS + 0.1 mL bacterial solution;

[0083] B) Single drug group: 0.7 mL PBS + 0.1 mL A / B drug + 0.1 mL PBS + 0.1 mL bacterial solution;

[0084] C) Combined group: 0.7 mL bacterial solution + 0.1 mL A drug + 0.1 mL B drug + 0.1 mL bacterial solution.

[0085] (4) This test set up 9 groups:

[0086] Staphylococcus aureus ATCC 29213: ①Control group; ②Lyme essential oil group: 1 / 2MIC (2.5%) Lyme essential oil; ③Lyme essential oil group: 1 / 4MIC (1.25%) Lyme essential oil; ④Silver nitrate group: 0.25μg / mL silver nitrate; ⑤Silver nitrate group: 0.5μg / mL silver nitrate; ⑥Combination group: 1 / 4MIC Lyme essential oil + 0.25μg / mL silver nitrate; ⑦Combination group: 1 / 4MIC Lyme essential oil + 0.5μg / mL silver nitrate; ⑧Combination group: 1 / 2MIC Lyme essential oil + 0.25μg / mL silver nitrate; ⑨Combination group: 1 / 2MIC Lyme essential oil + 0.5μg / mL silver nitrate;

[0087] (5) After the system is prepared, incubate the culture at 37°C with shaking at 180 rpm for 1 h.

[0088] (6) At 1 h, 100 μL of bacterial solution was aspirated from each tube and added to a 2 ml centrifuge tube containing 900 μL PBS buffer for ten-fold gradient dilution. After dilution, 25 μL was dropped on LB agar medium and incubated in a 37°C incubator for 16 to 18 h. The colonies were counted and the experimental results were statistically analyzed after three biological replicates.

[0089] The results are shown in Table 3 and Figure 2 As shown, the bacteria in the blank control group grew normally, indicating that Staphylococcus aureus ATCC 29213 could grow normally under the experimental conditions; in the single-drug group, there was no significant decrease in Staphylococcus aureus ATCC 29213 compared with the AgNO3 group alone, indicating that the inhibitory effect of the AgNO3 group alone on Staphylococcus aureus ATCC 29213 was not obvious; the same results also showed that the use of lime essential oil alone had a significant inhibitory effect on Staphylococcus aureus ATCC 29213; the results of the combined group showed that the synergistic effect of lime essential oil and silver nitrate had a significant bactericidal effect on Staphylococcus aureus ATCC 29213.

[0090] Table 3 Bactericidal effect of lime essential oil and silver nitrate on Staphylococcus aureus quality control strain ATCC 29213

[0091]

[0092]

[0093] Example 4 Evaluation of the combined bactericidal effect of lime essential oil and silver nitrate on multidrug-resistant Escherichia coli NP109

[0094] 1. Experimental materials:

[0095] (1) Experiment: Cool the autoclaved LB broth, PBS buffer, and ultrapure water for later use.

[0096] (2) Lime essential oil: Guangzhou Wuyuan Essential Oil Co., Ltd.

[0097] Silver nitrate: McLean brand, MW169.87

[0098] Test strain: multidrug-resistant Escherichia coli NP109 (laboratory collection).

[0099] 2. Preparation before the test:

[0100] (1) The multidrug-resistant Escherichia coli NP109 was subcultured on agar medium and cultured to an appropriate size.

[0101] (2) Use a precision balance to weigh 0.1 g of silver nitrate powder in a centrifuge tube, add 10 mL of sterile water to dissolve it, and the concentration of silver nitrate is 10 mg / mL. Vortex mix and filter. Pass the filtrate through a 0.22 μm diameter filter membrane for physical sterilization, divide it into sterilized centrifuge tubes, and store it in a -20°C freezer.

[0102] 3. Sterilization experiment:

[0103] (1) Inoculate a single colony of multidrug-resistant Escherichia coli NP109 in a centrifuge tube containing 20 mL of LB broth, place the tube in a 37°C shaker at 180 rpm for 3 h, and then remove the tube.

[0104] (2) Centrifuge the cultured bacterial solution at 5500 rpm for 3 min, resuspend in an equal volume of PBS buffer and dilute 10-fold. 7 CFU / mL, for future use.

[0105] (3) This experiment uses a 1 mL system and adds the bacterial solution in (2):

[0106] A) Control group: 0.7 mL PBS + 0.2 mL PBS + 0.1 mL bacterial solution;

[0107] B) Single drug group: 0.7 mL PBS + 0.1 mL A / B drug + 0.1 mL PBS + 0.1 mL bacterial solution;

[0108] C) Combined group: 0.7 mL bacterial solution + 0.1 mL A drug + 0.1 mL B drug + 0.1 mL bacterial solution.

[0109] (4) This test set up 9 groups:

[0110] Escherichia coli resistant bacteria NP109: ① control group; ② group with lime essential oil alone: ​​1 / 2MIC (2.5%) lime essential oil; ③ group with lime essential oil alone: ​​1 / 4MIC (1.25%) lime essential oil; ④ group with silver nitrate alone: ​​0.25μg / mL silver nitrate; ⑤ group with silver nitrate alone: ​​0.5μg / mL silver nitrate; ⑥ combined medication group: 1 / 4MIC lime essential oil + 0.25μg / mL silver nitrate; ⑦ combined medication group: 1 / 4MIC lime essential oil + 0.5μg / mL silver nitrate; ⑧ combined medication group: 1 / 2MIC lime essential oil + 0.25μg / mL silver nitrate; ⑨ combined medication group: 1 / 2MIC lime essential oil + 0.5μg / mL silver nitrate;

[0111] (5) After the system is prepared, incubate the culture at 37°C with shaking at 180 rpm for 1 h.

[0112] (6) At 1 h, 100 μL of bacterial solution was aspirated from each tube and added to a 2 ml centrifuge tube containing 900 μL PBS buffer for ten-fold gradient dilution. After dilution, 25 μL was dropped on LB agar medium and incubated in a 37°C incubator for 16 to 18 h. The colonies were counted and the experimental results were statistically analyzed after three biological replicates.

[0113] The results are shown in Table 4 and Figure 3 As shown, the bacteria in the blank control group grew normally, indicating that the multidrug-resistant Escherichia coli NP109 could grow normally under the experimental conditions; in the single-drug group, the multidrug-resistant Escherichia coli NP109 did not decrease significantly compared with the essential oil group alone, indicating that the essential oil group alone had no obvious inhibitory effect on the multidrug-resistant Escherichia coli NP109; the same results also showed that the use of AgNO3 alone had a significant inhibitory effect on the multidrug-resistant Escherichia coli NP109; the results of the combined group showed that the synergistic effect of lime essential oil and silver nitrate had a significant bactericidal effect on the multidrug-resistant Escherichia coli NP109.

[0114] Table 4 Bactericidal effect of lime essential oil and silver nitrate on multidrug-resistant Escherichia coli NP109

[0115]

[0116]

[0117] Example 5 Evaluation of the deodorizing effect of lime essential oil combined with silver nitrate

[0118] 1. Experimental materials:

[0119] (1) Test: Prepare a portable gas detector and a sealed aluminum box for use.

[0120] (2) Lime essential oil: Guangzhou Wuyuan Essential Oil Co., Ltd.; Silver nitrate: McLean brand, MW169.87

[0121] The test simulates the odor of a farm: ammonia.

[0122] 2. Preparation before the test:

[0123] (1) Debug the portable gas detector and complete the self-test.

[0124] 3. Deodorization experiment:

[0125] (1) Turn on the gas detector, put it into the detection box after debugging, and wait for 3 minutes to ensure that the instrument is stable.

[0126] (2) The dosage of ammonia water used in this experiment was 10 μL, and the liquid was dripped on the fixed site of the detection box: Control group: 10 μL ammonia water.

[0127] (3) This experiment set up 9 groups:

[0128] Ammonia: ①Control group; ②Lime essential oil group: 1 / 2MIC (2.5%) lime essential oil; ③Lime essential oil group: 1 / 4MIC (1.25%) lime essential oil; ④Silver nitrate group: 0.25μg / mL silver nitrate; ⑤Silver nitrate group: 0.5μg / mL silver nitrate; ⑥Combination group: 1 / 2MIC lime essential oil + 0.25μg / mL silver nitrate; ⑦Combination group: 1 / 2MIC lime essential oil + 0.5μg / mL silver nitrate; ⑧Combination group: 1 / 4MIC lime essential oil + 0.25μg / mL silver nitrate; ⑨Combination group: 1 / 4MIC lime essential oil + 0.5μg / mL silver nitrate;

[0129] (4) After the liquid is added, start the test and record the test value every 3 minutes;

[0130] (5) Result judgment: 0-19 means below the warning line, no alarm; 20-49 means low-level alarm; 50-100 means high-level alarm. (This value directly represents the high and low concentration data value of ammonia degradation)

[0131] Table 5 Deodorization effect of lime essential oil and silver nitrate on ammonia water

[0132]

[0133] The results are shown in Table 5. The detection value in the blank control group was 100, indicating that the odor detection was stable under the experimental conditions. The results of the single-drug group and the combined group showed that the separate and combined effects of lime essential oil and silver nitrate had significant deodorizing effects on ammonia water, but the combined effect had a better deodorizing effect on ammonia water.

[0134] Obviously, the above embodiments of the present invention are only examples for clearly illustrating the technical solution of the present invention, and are not intended to limit the specific implementation methods of the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the claims of the present invention shall be included in the protection scope of the claims of the present invention.

Claims

1. An antibacterial deodorizing composition, characterized in that: Made from lime essential oil and silver nitrate.

2. The antibacterial deodorizing composition according to claim 1, characterized in that: In the essential oil complex, the concentration of lime essential oil is 1%-5%, and the concentration of silver nitrate is 0.25-0.5 μg / mL.

3. Use of the antibacterial and deodorizing composition according to claim 1 or 2 in the preparation of antibacterial and deodorizing products.

4. The use according to claim 3, characterized in that: The antibacterial targets of the product are Escherichia coli, multi-drug resistant Escherichia coli and / or Staphylococcus aureus.

5. The use according to claim 3, characterized in that: The deodorizing object of the product is ammonia gas or ammonia water.

6. The use according to claim 3, characterized in that: The product is in the form of a solid, liquid, pump spray or semisolid gel.

7. An antibacterial and deodorizing product, characterized in that: The invention comprises the antibacterial and deodorizing composition according to claim 1 or 2.

8. The antibacterial and deodorizing product according to claim 7, characterized in that: The solvent of the antibacterial and deodorizing product is water.

9. The antibacterial and deodorizing product according to claim 8, characterized in that: The concentration of the lime essential oil is 1-5%.

10. A bacteriostatic and deodorizing method, characterized in that: The antibacterial and deodorizing product according to claim 9 is directly sprayed in the form of a diluted solution.

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