Application of extract in preparation of medicine or non-medicine composition for improving mitochondrial activity

Abstract

The embodiment of the invention provides application of an extract in preparation of a medicine or a non-medicine composition for improving mitochondrial activity. The mitochondria with improved activity can maintain its function and activity when facing pressure to ensure normal operation of cells without adverse effects of external or internal pressure.

Description

Technical Field

[0001] The present invention relates to the use of an extract for preparing a pharmaceutical or non-pharmaceutical composition for enhancing mitochondrial activity, and particularly relates to the use of a honeysuckle extract for preparing a pharmaceutical or non-pharmaceutical composition for enhancing mitochondrial activity. Background Art

[0002] Mitochondria are the primary sites within cells for oxidative phosphorylation and the synthesis of adenosine triphosphate (ATP). Because ATP is the energy source for cellular activity, mitochondria are also known as the "cell's energy factory." In addition to providing energy for cells, mitochondria also participate in processes such as cell differentiation, cell signaling, and apoptosis, and they have the ability to regulate the cell growth cycle.

[0003] However, some byproducts produced by mitochondria during oxidative phosphorylation are harmful to them, such as reactive oxygen species (ROS), including superoxide anion (O2·-), perhydroxyl radical (HO2·), and hydrogen peroxide (H2O2). ROS are highly biochemically reactive and can easily cause oxidative damage to cells or mitochondria. Damaged mitochondria can negatively impact cellular energy supply and growth. Over time, severely damaged mitochondria release cytochrome c (Cyt c), caspase-like proteases, and procaspase-2, 3, 8, and 9, triggering mitochondrial collapse. They also release apoptosis-related signaling factors, including proteases from the B cell lymphoma / leukemia-2 (Bcl-2) protease family, apoptotic protease activating factor-1 (Apaf-1), the p53 gene, and the serine protease Omi / HtrA2, further triggering apoptosis. Therefore, enhancing mitochondrial stress resistance, protecting and repairing mitochondria to maintain their function, and mitigating mitochondrial collapse has become a critical issue. Summary of the Invention

[0004] The embodiments of the present invention provide methods for utilizing extracts to enhance mitochondrial activity, thereby improving the ability of mitochondria to cope with stress, and thereby maintaining the function and activity of mitochondria under stress.

[0005] An embodiment of the present invention provides a use of an extract for preparing a pharmaceutical or non-pharmaceutical composition for enhancing mitochondrial activity, wherein the extract is a Lonicera Japonica extract.

[0006] The extract provided in an embodiment of the present invention is used to prepare a medical or non-medicinal composition for improving mitochondrial activity, wherein the extract is a honeysuckle extract. The honeysuckle extract can improve the mitochondrial pre-stored oxygen consumption capacity, maximum oxygen consumption capacity and adenosine triphosphate synthesis capacity, reduce mitochondrial hydrogen ion leakage, improve the mitochondrial adenosine triphosphate coordination efficiency and improve the mitochondrial bioenergetic health index. The mitochondria whose activity is enhanced by the honeysuckle extract can maintain their function and activity in the face of stress, thereby ensuring the normal functioning of cells without being adversely affected by external or internal stress. BRIEF DESCRIPTION OF THE DRAWINGS

[0007] Figure 1 HPLC chromatogram of honeysuckle extract.

[0008] Figure 2 This is the near-infrared spectrum of honeysuckle extract processed by Savitsky-Golay smoothing mode.

[0009] Figure 3 The results of cytotoxicity test of honeysuckle extract at different concentrations.

[0010] Figure 4 A measure of the oxygen consumption required by mitochondria to overcome hydrogen ion leak.

[0011] Figure 5 It is a measurement of the oxygen consumption of mitochondria to synthesize adenosine triphosphate.

[0012] Figure 6 It is a measurement of mitochondrial pre-stored oxygen consumption.

[0013] Figure 7 It is the measurement result of the maximum oxygen consumption of mitochondria.

[0014] Figure 8 is the mitochondrial ATP incorporation efficiency. DETAILED DESCRIPTION

[0015] The following detailed description of the features and advantages of the present invention is intended to enable those skilled in the art to understand the technical content of the present invention and implement it accordingly. Furthermore, based on the disclosure of this specification, the claims, and the accompanying drawings, those skilled in the art can readily understand the relevant objectives and advantages of the present invention. The following examples further illustrate the concepts of the present invention and are not intended to limit the scope of the present invention in any way.

[0016] Honeysuckle (Lonicera Japonica) is a perennial semi-evergreen climbing vine of the genus Lonicera in the family Caprifoliaceae. It is distributed in temperate and subtropical regions of North America, Europe, Asia, and northern Africa. The buds and petals of honeysuckle contain chlorogenic acid, isochlorogenic acid, acid), ginnol, β-sitosterol, stigmasterol, β-sitosterol-β-D-glucoside, stigmasterol-D-glucoside, and essential oil components, including linalool, cis-2,6,6-trimethyl-2-vinyl-5-hydroxytetrahydrofuran, ethylpalmitate, 1,1-bicyclohexane 1,1-bicyclohexyl, 3-methyl-2-(2-pentenyl)-2-cyclopenten-1-one, trans-trans-farnesol, ethyllinolemate, β-cubebene, cis-3-hexen-1-ol, α-erpineol, benzylbenzoate, 2-methyl-1-butanol, benzyl alcohol, phenethylalcohol, cis-linalool oxide, eugenol, and carvacrol.

[0017] The main active ingredient in honeysuckle is chlorogenic acid, which has biological activities including inhibition of hyaluronidase and glucose-6-phosphatase, free radical scavenging, anti-lipid peroxidation, anti-mutagenicity, hepatoprotective and choleretic properties, antibacterial, antiviral, and antispasmodic effects. In Traditional Chinese Medicine, honeysuckle has the ability to clear heat and detoxify, and dissipate wind-heat. It is used to treat carbuncles, furuncles, sore throat, erysipelas, dysentery caused by toxic heat in the blood, wind-heat colds, and fever caused by epidemic diseases. It has antibacterial, anti-inflammatory, antiviral, and lipid-lowering effects.

[0018] The extract of one embodiment of the present invention is a honeysuckle extract, which can be obtained by extracting the petals of honeysuckle with water and drying them. In detail, first, the petals of honeysuckle are washed three times with clean water. Then, the petals of honeysuckle are extracted with water three times at 60°C to 80°C, with the volume ratio of petals to water being approximately 1:3, and each extraction lasting approximately three hours. The extracted solution is cooled to room temperature. Then, the cooled extraction solution is sterilized and spray-dried at 80°C for 20 hours to obtain a powdered extract. Finally, the powdered extract is filtered using an 80-mesh sieve to obtain a honeysuckle extract having a yellow-brown powdery appearance. In other embodiments, the honeysuckle extract can also be extracted using other polar solvents, or the petals can be chopped or ground before extraction.

[0019] The honeysuckle extract obtained by water extraction in the above manner contains chlorogenic acid. The weight percentage concentration of chlorogenic acid may be 20% to 30%. In some embodiments, the weight percentage concentration of chlorogenic acid in the honeysuckle extract may be 25%. In other embodiments, the honeysuckle extract extracted with water may contain a weight percentage concentration of about 25% chlorogenic acid, a weight percentage concentration of about 17.7% secologanic acid, and a weight percentage concentration of about 0.5% galuteolin. The honeysuckle extract is analyzed using HPLC to obtain the results shown in Table 1 and Table 2. Figure 1 , Figure 1 The HPLC chromatogram of honeysuckle extract, 1 to 3 in Table 1 correspond to Figure 1 The signal peaks 1 to 3 in the sample were analyzed by infrared spectrometer and processed by Savitsky-Golay smoothing mode to obtain the peaks 1 to 3 in the sample. Figure 2 , Figure 2 This is the near-infrared spectrum of honeysuckle extract processed by Savitsky-Golay smoothing mode.

[0020] Table 1

[0021] serial number Element Average concentration Weight percentage 1 Strychnine 0.3795mg / mL 17.7％ 2 Chlorogenic acid 0.5340mg / mL 25％ 3 Luteolin 10.69 μg / mL 0.5％

[0022] In some embodiments of the present invention, providing cells with a honeysuckle extract at a concentration of 200 μg / mL to 500 μg / mL can increase mitochondrial activity, specifically by increasing mitochondrial pre-stored oxygen consumption capacity, maximum oxygen consumption capacity, and ATP synthesis capacity, reducing mitochondrial hydrogen ion leakage, increasing mitochondrial ATP incorporation efficiency, and improving mitochondrial bioenergetics health index. In another embodiment, the concentration of the honeysuckle extract can be 200 μg / mL to 250 μg / mL. In other embodiments, the concentration of the honeysuckle extract can be 250 μg / mL to 500 μg / mL.

[0023] The method of providing the honeysuckle extract to cells is, for example, to orally ingest the honeysuckle extract. When the honeysuckle extract is provided to cells by oral administration, the effective dose of the honeysuckle extract is 2.162 grams to 5.406 grams. The effective dose here is obtained by converting the effective dose in cell experiments to the kilograms of the human body. The conversion formula is as follows: Effective dose in human body = Effective dose in cell experiments × Mouse weight × Conversion coefficient × Human kilograms. The conversion coefficient is obtained by looking up the table of conversion coefficients of dose per kilogram of body weight for animals and humans. When the mouse weighs 20 grams and the human kilogram is 60 kilograms, the conversion coefficient is 9.01. In another embodiment, the effective dose of the honeysuckle extract is 2.162 grams to 2.703 grams. In other embodiments, the effective dose of the honeysuckle extract is 2.703 grams to 5.406 grams.

[0024] To facilitate oral ingestion of honeysuckle extract, the extract can be prepared into a processed honeysuckle extract product in a liquid, solid, granular, powdered, paste, or gel form. In some embodiments of the present invention, the processed honeysuckle extract product may also contain other ingredients or additives, such as carriers, diluents, adjuvants, excipients, or flavoring agents, without affecting the efficacy and objectives of the present invention. Excipients enhance the convenience and practicality of the preparation, while flavoring agents enhance the flavor of the preparation.

[0025] Examples of excipients include starches such as wheat starch, rice starch, corn starch, potato starch, dextrin, and cyclodextrin; crystalline celluloses; sugars such as lactose, glucose, granulated sugar, reduced maltose, maltose, fructooligosaccharides, and emulsified oligosaccharides; and sugar alcohols such as sorbitol, erythritol, xylitol, lactitol, and mannitol.

[0026] Flavoring agents include, for example, various fruit juice extracts such as longan extract, lychee extract, and grapefruit extract; various fruit juices such as apple juice, orange juice, and lemon juice; various flavors such as peach flavor, plum flavor, and yogurt flavor; various sweeteners such as acesulfame potassium, sucralose, erythritol, oligosaccharides, mannose, xylitol, and isomerized sugars; various acidulants such as citric acid, malic acid, tartaric acid, and gluconic acid; various tea ingredients such as green tea, oolong tea, Banaba tea, eucommia tea, Tieguanyin tea, coix tea, seven-leaf gall tea, wild rice tea, and kelp tea.

[0027] Furthermore, the honeysuckle extract composition of the embodiments of the present invention can be a pharmaceutical composition or a non-pharmaceutical composition, or can also be a health food. The honeysuckle extract or the composition comprising the honeysuckle extract can also be encapsulated in a capsule to facilitate oral ingestion of the honeysuckle extract. The honeysuckle extract or the composition comprising the honeysuckle extract can be encapsulated in a hard capsule in the form of a dry powder, or can be encapsulated in a soft capsule in the form of a solution, suspension, paste, powder, or granules.

[0028] Oils and fats used to dissolve or disperse the honeysuckle extract in the soft capsule include, for example, calyx oil, almond oil, linseed oil, fennel oil, perilla oil, olive oil, olive squalene, sweet orange oil, orange roughy oil, sesame oil, garlic oil, cocoa butter, pumpkin seed oil, chamomile oil, carrot oil, cucumber oil, tallow fatty acid, macadamia nut oil, cranberry oil, brown rice germ oil, rice oil, wheat germ oil, safflower oil, shea oil, liquid shea oil, perilla oil, soybean oil, evening primrose oil, camellia oil, corn oil, rapeseed oil, saw palmetto extract oil, coix seed oil, peach kernel oil, celery seed oil, castor oil, sunflower seed oil, grape seed oil, borage oil, macadamia nut oil, meadowfoam oil, oil), cottonseed oil, peanut oil, tortoise oil, mink oil, egg yolk oil, fish oil, palm oil, palm kernel oil, wood wax, coconut oil, long-chain / medium-chain / short-chain fatty acid triglycerides, diglycerides, tallow, lard, squalene, squalane, pristane, and hydrogenated products of these oils, etc.

[0029] In addition, colorants, preservatives, viscosity enhancers, binders, disintegrants, dispersants, stabilizers, gelling agents, antioxidants, surfactants, preservatives, pH adjusters and other additives that comply with the regulations of relevant units can also be added to the processed products of the honeysuckle extract composition in accordance with the dosage standards and processing and production requirements specified by the relevant units.

[0030] The following describes an experiment using a honeysuckle extract according to an embodiment of the present invention to enhance mitochondrial activity. The honeysuckle extract used in the experiment was obtained by extracting honeysuckle petals with water and drying them as described above, and the honeysuckle extract contained at least chlorogenic acid. The cells used in the experiment were skeletal muscle cells (C2C12). The cells were cultured using DMEM medium containing 10% fetal bovine serum (FBS). The cell subculture method was as follows: First, the cells were cultured to a certain volume, the culture medium was removed, and the cells were rinsed twice with phosphate-buffered saline (PBS). Next, trypsin was added and allowed to react with the cells at 37°C for 5 minutes, after which culture medium was added to terminate the trypsin reaction. Next, the cell-containing solution was centrifuged at 300g (relative centrifugal force, RCF) for 5 minutes, the supernatant was removed, and the precipitate was re-dissolved with culture medium. Finally, the cells were transferred to a cell culture flask (175T flask) for storage until subsequent experiments, where the cell count was 1×10 6 cells.

[0031] [Experiment 1] Cytotoxicity of Honeysuckle Extract

[0032] First, the cytotoxicity of honeysuckle extract was tested. Alamar blue is a detection reagent used to detect cell viability. The resazurin in the detection kit is a redox indicator. It is a non-toxic, cell membrane-permeable, low-fluorescence dark blue dye. When resazurin enters healthy cells, it is reduced to resorufin, a pink and highly fluorescent dye, due to the reducing environment in the living cells. Cell survival can be assessed by measuring the light absorbance or fluorescence value of resorufin produced in the cells. The higher the light absorbance or fluorescence value of resorufin, the higher the cell survival rate. The higher the cell survival rate, the healthier the cells and the stronger their proliferation ability. The stronger the cell proliferation ability, the more cells there are. Therefore, Alamar blue can be used as an indicator of cytotoxicity to determine cell survival rate and cell proliferation rate.

[0033] The following details the testing process for the cytotoxicity of honeysuckle extract. On the first day, cells were cultured in a 96-well plate with a total volume of cells and culture medium of 200 μl and 10,000 cells per well for one day. On the second day, honeysuckle extract was added to each well at concentrations of 50, 100, 200, 250, 500, and 1000 μg / ml, respectively, and the cells were cultured at 37°C for one day. On the third day, cytotoxicity was tested using Alamar blue. Specifically, Alamar blue was prepared into a 10% weight percent solution in a dark environment and added to the plate at a volume of 100 μl per well. The cells were incubated at 37°C for 3 to 4 hours, and the absorbance and fluorescence values ​​(OD530 / 590) were measured using an ELISA reader. This method obtains the cell viability after treatment with the honeysuckle extract, which represents the cytotoxicity of the honeysuckle extract.

[0034] The experimental results revealed Figure 3 , Figure 3 The results of a cytotoxicity test of different concentrations of honeysuckle extract are shown. The control group represents cells not treated with honeysuckle extract. The vertical axis represents cell survival relative to the control group. ***(P<0.001) indicates a highly significant difference relative to the control group.

[0035] like Figure 3 As shown, honeysuckle extract at concentrations below 500 μg / mL had no effect on cell viability, indicating that honeysuckle extract at concentrations below 500 μg / mL is non-cytotoxic. Therefore, honeysuckle extracts at concentrations of 200, 250, and 500 μg / mL were selected for subsequent experiments in Examples 1 to 3 of the present invention.

[0036] [Experiment 2] Honeysuckle extract enhances mitochondrial activity

[0037] Next, we conducted an experiment to investigate the use of honeysuckle extract to enhance mitochondrial activity. This experiment used tert-butyl hydroperoxide (tBHP) as a substance that induces oxidative stress damage, aging, and inhibits mitochondrial activity.

[0038] The following details the experimental process for enhancing mitochondrial activity using honeysuckle extract. On the first day, cells were cultured in a 24-well hippocampal plate with a total volume of cells and culture medium of 100 μl, with 25,000 cells per well. 150 μl of culture medium was then added and the cells were cultured for one day. On the second day, honeysuckle extract was added to each well at concentrations of 200, 250, and 500 μg / ml, with a total volume of 250 μl. The cells were cultured with honeysuckle extract under these conditions for one day. On the third day, the culture medium was replaced, and 100 μM tBHP was added to each well to allow the cells to react for one hour. The culture medium in the plate was then replaced with 675 μl of supernatant medium (DMEM without fetal bovine serum). After incubation for one hour in a CO2-free incubator, oxygen consumption of the cells in the wells was measured using a hippocampal bioenergetics instrument.

[0039] The measurement principle and process of the Seahorse Bioenergetics Meter are as follows. First, the basal oxygen consumption of the cells in the well is measured. Next, an ATP synthase inhibitor is added to inhibit mitochondrial ATP production. The reduced oxygen consumption is the oxygen consumption for ATP synthesis (ATP production). Next, an appropriate concentration of an anti-coupling agent is added to allow the mitochondria to idle at extreme conditions without disrupting the electron transport chain in the mitochondrial inner membrane to assess mitochondrial maximum oxygen consumption (maximal respiration). Finally, an electron transport chain inhibitor is added to completely shut down mitochondrial oxygen consumption, thereby determining the background value of the measurement, namely non-mitochondrial oxygen consumption. Mitochondrial basal oxygen consumption (basal respiration) is equal to the basal oxygen consumption of the cell minus non-mitochondrial oxygen consumption. Mitochondrial basal oxygen consumption minus oxygen consumption for ATP synthesis equals oxygen consumption to overcome proton leakage (proton leakage). Mitochondrial spare respiratory capacity (spare respiratory capacity) is equal to mitochondrial maximum oxygen consumption minus mitochondrial basal oxygen consumption. The mitochondrial ATP coupling efficiency is equal to the oxygen consumption for synthesizing ATP divided by the basal oxygen consumption of the mitochondria.

[0040] The experimental results are shown in Table 2 and Figures 4 to 8 , Figure 4 It is a measure of the oxygen consumption required by mitochondria to overcome hydrogen ion leakage. Figure 5 It is the measurement result of oxygen consumption for mitochondrial synthesis of adenosine triphosphate. Figure 6 This is the measurement result of mitochondrial pre-stored oxygen consumption. Figure 7 is the measurement result of the maximum oxygen consumption of mitochondria. Figure 8The control group consisted of cells that were not treated with tBHP and honeysuckle extract, the control group consisted of cells that were treated with tBHP but not honeysuckle extract, and the experimental group consisted of cells that were treated with the honeysuckle extract of the example and tBHP (Tables 2 to 3 and Figures 4 to 8 In the examples 1 to 3). Figures 4 to 7 In the figure, the vertical axis is the number of picomoles of oxygen consumed per minute (pmol / min). Figure 8 In the figure, the vertical axis represents the ATP incorporation efficiency in percentage (%). *** (P < 0.001) indicates a very significant difference relative to the control group, and ### (P < 0.001) indicates a very significant difference relative to the control group.

[0041] Table 2

[0042]

[0043] like Figure 4 As shown, in the measurement of mitochondrial hydrogen ion leakage, the comparison group had higher values ​​than the control group, indicating that the inner mitochondrial membrane of the comparison group was damaged, requiring more oxygen consumption to overcome hydrogen ion leakage. In contrast, the experimental group treated with the honeysuckle extracts of Examples 1 to 3 had lower values ​​than the comparison group and was close to the control group, indicating that the honeysuckle extract enhanced mitochondrial activity, which is equivalent to the honeysuckle extract's function of protecting and repairing mitochondria under oxidative stress, resulting in less damage to the mitochondrial inner membrane.

[0044] like Figure 5 As shown, in the measurement results of mitochondrial ATP synthesis, the comparison group was lower than the control group, indicating that the mitochondrial ability to synthesize ATP is reduced under oxidative stress, and the energy generated by the mitochondria is reduced. In contrast, the experimental group treated with the honeysuckle extracts of Examples 1 to 3 was higher than the comparison group and close to the control group, indicating that the honeysuckle extract enhanced mitochondrial activity and the ability to synthesize ATP under oxidative stress, thereby generating sufficient energy for cellular use and maintaining a level similar to that of the control group.

[0045] like Figure 6 As shown in the results of the pre-stored oxygen consumption measurement, the comparison group was lower than the control group, indicating that the pre-stored oxygen consumption capacity of mitochondria was reduced under oxidative stress. In contrast, the experimental group treated with the honeysuckle extracts of Examples 1 to 3 was higher than the comparison group and close to the control group, indicating that the honeysuckle extract enhanced mitochondrial activity and the pre-stored oxygen consumption capacity of mitochondria under oxidative stress. This increase in pre-stored oxygen consumption capacity indicates that mitochondria have better resilience to stress and can maintain a level similar to that of the control group.

[0046] like Figure 7As shown, in the measurement results of maximum oxygen consumption, the comparison group was lower than the control group, indicating that the maximum oxygen consumption capacity of mitochondria was reduced under oxidative stress. In contrast, the experimental group treated with the honeysuckle extracts of Examples 1 to 3 was higher than the comparison group and close to the control group, indicating that the honeysuckle extract enhanced mitochondrial activity and the maximum oxygen consumption capacity of mitochondria under oxidative stress was also improved, and was able to maintain a level similar to that of the control group.

[0047] like Figure 8 As shown in the results of ATP incorporation efficiency, the comparison group was lower than the control group, while the experimental group treated with the honeysuckle extract of Examples 1 to 3 was higher than the comparison group and close to the control group, indicating that the activity of mitochondria was enhanced by the honeysuckle extract, and the ATP incorporation efficiency of mitochondria was also enhanced under oxidative stress and could be maintained at a level similar to that of the control group.

[0048] Based on the measurements from the Seahorse Bioenergetic Meter, the Bioenergetic Healthy Index (BHI) can be calculated from mitochondrial oxygen consumption. The BHI is an energy metabolism assessment indicator calculated using mitochondrial energy metabolism data. BHI = log [oxygen consumption for ATP synthesis × pre-stored oxygen consumption] / [oxygen consumption to overcome hydrogen ion leakage × non-mitochondrial oxygen consumption]. A high BHI indicates high mitochondrial activity and a strong ability to cope with stress. Therefore, the BHI can be used as an indicator to assess mitochondrial and cellular health.

[0049] The bio-energy health index calculated from the above experimental results is shown in Table 3. As shown in Table 3, compared with the control group, the bio-energy health index was improved after treatment with the honeysuckle extract of the embodiment, indicating that the health of mitochondria and cells was improved.

[0050] Table 3

[0051]

[0052] The above experimental results indicate that honeysuckle extract at concentrations below 500 μg / mL is non-toxic to cells. Furthermore, honeysuckle extract can enhance mitochondrial activity, specifically by increasing mitochondrial pre-stored oxygen consumption capacity, maximum oxygen consumption capacity, and ATP synthesis capacity, reducing mitochondrial hydrogen ion leakage, increasing mitochondrial ATP incorporation efficiency, and improving mitochondrial bioenergetics.

[0053] The extract provided in an embodiment of the present invention is used to prepare a medical or non-medicinal composition for improving mitochondrial activity, wherein the extract is a honeysuckle extract. The honeysuckle extract can improve the mitochondrial pre-stored oxygen consumption capacity, maximum oxygen consumption capacity and adenosine triphosphate synthesis capacity, reduce mitochondrial hydrogen ion leakage, improve the mitochondrial adenosine triphosphate coordination efficiency and improve the mitochondrial bioenergetic health index. The mitochondria whose activity is enhanced by the honeysuckle extract can maintain their function and activity in the face of stress, thereby ensuring the normal functioning of cells without being adversely affected by external or internal stress.

Claims

1. Use of an extract for preparing a pharmaceutical or non-pharmaceutical composition for enhancing mitochondrial activity, wherein the extract is a Lonicera Japonica extract.

2. The use according to claim 1, wherein increasing mitochondrial activity comprises increasing the pre-stored oxygen consumption capacity of the mitochondria.

3. The use according to claim 1, wherein increasing mitochondrial activity comprises increasing the maximum oxygen consumption capacity of the mitochondria.

4. The use according to claim 1, wherein increasing mitochondrial activity comprises increasing the ATP synthesis capacity of the mitochondria.

5. The use according to claim 1, wherein increasing mitochondrial activity comprises reducing hydrogen ion leakage of the mitochondria. The use according to claim 1 , wherein increasing mitochondrial activity comprises increasing the ATP incorporation efficiency of the mitochondria.

7. The use according to claim 1, wherein increasing mitochondrial activity comprises increasing the bioenergetic health index of the mitochondria.

8. The use according to claim 1, wherein the honeysuckle extract comprises chlorogenic acid.

9. The use according to claim 8, wherein the weight percentage concentration of the chlorogenic acid in the honeysuckle extract is 20% to 30%.

10. The use according to claim 1, wherein the honeysuckle extract is obtained by extracting honeysuckle petals with water and drying, and the volume ratio of honeysuckle petals to water is 1:

3.

11. The use according to claim 1, wherein in the composition, the concentration of the honeysuckle extract is 200 μg / ml to 500 μg / ml.

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