Feed additive for improving intramuscular fat deposition in late fattening period of Holstein capons and application of feed additive

Abstract

The invention provides a feed additive for improving intramuscular fat deposition in the later fattening period of Holstein capons and application, and belongs to the technical field of feed additives. The feed additive consists of the following components in parts by weight: 5-25 parts of guanidinoacetic acid, 10-25 parts of methionine, 5-20 parts of vitamin C, 5-25 parts of vitamin E, 10-25 parts of compound vitamin B, 10-35 parts of rumen bypass fat and 10-35 parts of glucose. The invention further provides a method for improving intramuscular fat deposition in the later fattening period of the caponized cattle. According to the method, the caponized cattle are fed by adding the feed additive into daily ration. The feed additive provided by the invention can effectively regulate and control the energy state of cells, has the functions of changing substance metabolism, regulating and controlling glycolysis, promoting fat synthesis in muscular tissues, reducing subcutaneous fat synthesis and the like, and improves the muscle protein synthesis capability and the intramuscular fat content in the late fattening period of castrated cattle.

Description

Technical Field

[0001] The invention belongs to the technical field of feed additives, and in particular relates to a feed additive for improving intramuscular fat deposition in the late fattening period of Holstein steers, and an application and method thereof. Background Art

[0002] As consumers' living standards improve, people have higher and higher requirements for beef quality, and marbled beef rich in intramuscular fat deposition has begun to be favored by more consumers. Intramuscular fat (IMF) content is an important indicator for evaluating the grade of marbled beef. IMF content is closely related to quality indicators such as beef juiciness, flavor and meat color. The higher the IMF content, the higher the beef marbling (BMS) grade {Japanese Feeding Standards • Beef Cattle (2008 edition, translated by Cao Binghai, 1st edition, December 2009, page 89)}. In addition to breed and gender factors, the factors that affect the intramuscular fat deposition of beef cattle can also achieve the effect of promoting IMF deposition through feed nutrition regulation technology in the late fattening period.

[0003] Studies have shown that Holstein steers can produce marbled beef by straight fattening until 18 months of age. However, the weight gain rate begins to decline at 15 months of age, and the IMF deposition capacity begins to increase, including the deposition of subcutaneous and visceral fat. In view of this, how to further improve the BMS grade of Holstein steers in the late fattening period and reduce the deposition of subcutaneous fat and visceral fat through nutritional regulation technology is a very important issue, but there are no reports of additives and methods in the prior art that can effectively increase the deposition of intramuscular fat in steers between 15 and 18 months of age. Summary of the invention

[0004] The object of the present invention is to provide a feed additive for improving intramuscular fat deposition in the late fattening period of Holstein steers, which is used to promote fat deposition in the diet of Holstein steers in the late fattening period, thereby achieving the purpose of improving the marbling grade of beef.

[0005] Another object of the present invention is to provide a method for increasing intramuscular fat deposition in steers.

[0006] To achieve the above object, the present invention adopts the following technical solutions: A feed additive for improving intramuscular fat deposition in the late fattening period of Holstein steers, comprising, by weight, 5-25 parts of guanidinoacetic acid (GAA), 10-25 parts of methionine, 8-20 parts of vitamin C, 10-22 parts of vitamin E, 10-25 parts of vitamin B complex, 10-35 parts of rumen bypass fat and 10-35 parts of glucose.

[0007] Furthermore, preferably, GAA is 8 to 20 parts, methionine is 10 to 22 parts, vitamin C is 12 to 18 parts, vitamin E is 10 to 20 parts, vitamin B complex is 10 to 20 parts, rumen bypass fat is 10 to 30 parts, and glucose is 15 to 25 parts.

[0008] Furthermore, the most preferred content is 10 parts of GAA, 10 parts of methionine, 12 parts of vitamin C, 13 parts of vitamin E, 15 parts of vitamin B complex, 20 parts of rumen bypass fat, and 20 parts of glucose.

[0009] Furthermore, the vitamin B complex is a mixture of vitamin B1, vitamin B2 and vitamin B12, preferably a mixture of vitamin B1, vitamin B2 and vitamin B12 in a weight ratio of 1:1:1.5.

[0010] A feed containing a feed additive as described above.

[0011] The feed as described above, further, the content of the feed additive is 0.6-1.0% of the weight of the feed.

[0012] Use of the feed additive or feed as described above in improving intramuscular fat deposition in late fattening period of steers.

[0013] Use of the feed additive as described above in preparing feed for improving intramuscular fat deposition in the late fattening period of steers.

[0014] Preferably, the feed additive is used in an amount of 0.6-1.0% of the feed weight.

[0015] Preferably, the steer is a fattening Holstein steer at the age of 15 to 18 months.

[0016] A method for increasing intramuscular fat deposition in steers, comprising adding a feed additive as described above to the diet or feeding the steers with a feed as described above.

[0017] Furthermore, the feeding objects are straight-fattening Holstein steers at the age of 15 to 18 months.

[0018] Furthermore, the feed additive is fed for 90 to 120 days.

[0019] A method for increasing intramuscular fat deposition in linear fattening Holstein steers in the late fattening stage (15 to 18 months of age), comprising adding the feed additive as described above to the steer diet and feeding the linear fattening Holstein steers of 15 to 18 months of age, that is, the method is applied to the late stage of linear fattening of Holstein steers.

[0020] Furthermore, in the method as described above, the feed additive is added to the diet in an amount of 0.6-1.0% by weight of the diet.

[0021] Furthermore, in the method as described above, it is preferred that the feed additive is fed for 90 to 120 days.

[0022] The beneficial effects of the present invention are: The present invention provides a feed additive for improving intramuscular fat deposition in the late fattening stage of Holstein steers. The feed additive is added to feed for feeding steers in the late fattening stage (15-18 months old), which is beneficial to improving the deposition of intramuscular fat. The feed additive can effectively regulate the energy state of cells, change material metabolism, regulate glycolysis, promote fat synthesis in muscle tissue, reduce physiological functions such as subcutaneous fat synthesis, and improve muscle protein synthesis capacity and intramuscular fat content in the late fattening stage of Holstein steers. High-energy feed is prone to lead to VC deficiency, so the addition of VC, VB and VE in the feed additive is helpful to regulate sugar metabolism and promote fat synthesis, ensure rumen health, and improve the body's antioxidant capacity in the late fattening stage of Holstein steers.

[0023] The method provided by the present invention is used to improve the intramuscular fat deposition of linear fattening Holstein steers in the late fattening period (15-18 months of age). By adding a feed additive for improving the intramuscular fat deposition of steers in the late fattening period into the diet, the intramuscular fat deposition efficiency of the Holstein steers in the late fattening period is improved, the marbling grade of beef is improved, and the subcutaneous fat deposition is reduced, thereby improving the economic value of the Holstein fattening steers. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 This is a rating picture of the 6-7 rib longissimus dorsi muscle of a group in Example 6 of the present invention.

[0025] Figure 2 This is a rating picture of the 6-7 rib longissimus dorsi muscle of the two groups in Example 6 of the present invention.

[0026] Figure 3 These are rating pictures of the 6-7 rib longissimus dorsi muscles of the three groups in Example 6 of the present invention.

[0027] Figure 4 These are rating pictures of the 6-7 rib longissimus dorsi muscles of the four groups in Example 6 of the present invention.

[0028] Figure 5 This is a rating picture of the 6-7 rib longissimus dorsi muscle of Group A in Example 7 of the present invention.

[0029] Figure 6 This is a rating picture of the 6-7 rib longissimus dorsi muscle of Group B in Example 7 of the present invention.

[0030] Figure 7This is a rating picture of the 6-7 rib longissimus dorsi muscle of Group C in Example 7 of the present invention.

[0031] Figure 8 This is a rating picture of the 6-7 rib longissimus dorsi muscle of Comparative Example 1 of the present invention.

[0032] Fig. 9 This is a rating picture of the 6-7 rib longissimus dorsi muscle of Comparative Example 2 of the present invention.

[0033] Fig.10 This is a rating picture of the 6-7 rib longissimus dorsi muscle of Comparative Example 3 of the present invention.

[0034] Fig.11 This is a rating picture of the 6-7 rib longissimus dorsi muscle of Comparative Example 4 of the present invention. DETAILED DESCRIPTION

[0035] In order to improve the marbled beef grade (BMS) of 18-month-old Holstein steers, how to further improve the BMS grade of Holstein steers in the late fattening period (15-18 months old) through nutritional regulation technology, and reduce the deposition of subcutaneous fat and visceral fat in the late fattening period of Holstein steers, is of great significance for improving the fattening production performance, beef quality, and economic benefits of Holstein steers. After a large number of experiments, it was found that on the basis of a high-nutrient concentration diet, through the synergistic effect of GAA and Met, vitamin C (VC), vitamin E (VE), complex B vitamins (VB), rumen fat and glucose, the protein and energy status of Holstein steers can be effectively regulated, the ability to synthesize muscle protein and the ability to deposit intramuscular fat can be improved, the synthesis of subcutaneous fat can be reduced, and the intramuscular fat content of steers in the late fattening period can be increased.

[0036] Methionine (Met) used in the present invention: Met is the main limiting amino acid for the growth of most animals and participates in the body's protein metabolism. Supplementing Met in the diet can promote protein synthesis and cell proliferation and differentiation, and then promote the development of animal skeletal muscle, thereby effectively improving the meat production performance of animals, and can provide methyl for GAA to synthesize creatine. A large number of experimental studies have found that when the amount of Met added can cause a series of diseases such as animal growth inhibition and protein synthesis disorders, it can cause death in severe cases; because it is an essential amino acid for protein synthesis, too low a content will affect protein synthesis and reduce feed conversion efficiency; combined with the results of feeding tests, the preferred amount of methionine is 10 to 25 parts, and further, 10 to 22 parts is preferred.

[0037] Guanidine acetic acid (GAA): GAA can stimulate the hypothalamus by increasing the secretion of γ-aminobutyric acid in the body, promote the secretion of growth hormone-releasing hormone in the hypothalamus, and then promote the secretion of growth hormone by the pituitary gland, thus promoting the growth of animals; it can also increase the secretion of insulin in the body and increase feed intake. GAA is also the only precursor of creatine, and creatine needs to be synthesized with the participation of methyl donors and methyltransferases. However, studies have found that when the amount of GAA added exceeds 660ppm, methyltransferases no longer participate in metabolic synthesis, and the passage rate of GAA in the rumen is about 50%. Experiments have shown that the amount of GAA added to ruminant feed is 0.06~0.12% under the premise of methyl donors. Therefore, the preferred amount of GAA is 5~25 parts, and the preferred amount of GAA is 8~20 parts.

[0038] VC: It has antioxidant effects. Studies have found that adding VC can participate in the metabolism of cholesterol, amino acids and hormones, enhance immune function, and effectively secrete insulin.

[0039] VE: It has antioxidant and immunity-enhancing effects, promotes growth, and especially improves the antioxidant capacity of marbled beef.

[0040] VB group: related to a variety of catalytic enzymes, related to the energy metabolism of the nervous system and the synthesis of fatty acids and cholesterol in cell membranes, and is also a coenzyme for enzymes related to the synthesis of methionine.

[0041] Bypassed rumen fat and glucose: can improve the efficiency of intramuscular fat deposition. Studies have found that adding fat and glucose to the diet can significantly increase the fat content of the longissimus dorsi muscle, but it will reduce the body's antioxidant capacity. With the addition of glucose, the proportion of fatty acid synthesis in muscle tissue is greater than that in subcutaneous adipose tissue. Excessive amounts of added bypassed rumen fat and glucose will lead to increased feed costs and increase the risk of rumen poisoning. Therefore, the preferred amount of bypassed rumen fat is 10 to 35 parts, and the further preferred amount of bypassed rumen fat is 15 to 30 parts; the preferred amount of glucose is 10 to 35 parts, and the further preferred amount of glucose is 15 to 25 parts.

[0042] The following examples are used to further illustrate the present invention, but should not be construed as limiting the present invention. Without departing from the spirit and substance of the present invention, modifications or substitutions made to the present invention all belong to the scope of the present invention.

[0043] If not otherwise specified, the technical means used in the examples are conventional means well known to those skilled in the art. Unless otherwise specified, the raw materials used in the examples of the present invention can be commercially available products, such as GAA can be purchased from Beijing Junde Tongchuang Co., Ltd., rumen bypass fat can be purchased from Wilmar International Holdings Co., Ltd., methionine can be purchased from CJ Pharmaceuticals, and composite VB can be purchased from Xintai Jiahe Biotechnology Co., Ltd. Example 1

[0044] A feed additive for improving intramuscular fat deposition of Holstein steers in the late fattening period is provided. The following ingredients are weighed in parts by weight: 10 parts of GAA, 20 parts of methionine, 16 parts of vitamin C, 20 parts of vitamin E, and 14 parts of complex vitamin B1, wherein the complex vitamin B is prepared by vitamin B1: vitamin B2: vitamin B12 in a weight ratio of 1:1:1.5. 20 parts of rumen bypass fat and 20 parts of glucose are put into a stainless steel premixing tank and mixed for 10 minutes, and then put into a plastic bag and sealed for storage, so as to obtain a feed additive for fattening. Example 2

[0045] Weigh out by weight: 8 parts of GAA, 15 parts of methionine, 15 parts of vitamin C, 17 parts of vitamin E, 20 parts of compound VB, 18 parts of rumen bypass fat and 22 parts of glucose, put them into a stainless steel premixing tank and mix for 10 minutes, wherein the compound VB is prepared with vitamin B1: vitamin B2: vitamin B12 in a weight ratio of 1:1:1.5, put them into a plastic bag and seal them for storage to obtain a feed additive for fattening. Example 3

[0046] Weigh by weight: 20 parts of GAA, 22 parts of methionine, 10 parts of vitamin C, 20 parts of vitamin E, 10 parts of compound VB, 24 parts of rumen bypass fat and 15 parts of glucose, put them into a stainless steel premixing tank and mix for 10 minutes, wherein the compound VB is prepared with vitamin B1: vitamin B2: vitamin B12 in a weight ratio of 1:1:1.5, put them into a plastic bag and seal them for storage to obtain a feed additive for fattening. Example 4

[0047] Weigh by weight: 10 parts of GAA, 10 parts of methionine, 12 parts of vitamin C, 13 parts of vitamin E, 15 parts of compound VB, 20 parts of rumen-bypass fat and 20 parts of glucose, put them into a stainless steel premixing tank and mix for 10 minutes, wherein the compound VB is prepared with vitamin B1: vitamin B2: vitamin B12 in a weight ratio of 1:1:1.5, put them into a plastic bag and seal them for storage to obtain a feed additive for fattening. Example 5

[0048] Weigh out by weight: 18 parts of GAA, 14 parts of methionine, 16 parts of vitamin C, 14 parts of vitamin E, 17 parts of compound VB, 25 parts of rumen-bypass fat and 20 parts of glucose, put them into a stainless steel premixing tank and mix for 10 minutes, wherein the compound VB is prepared with vitamin B1: vitamin B2: vitamin B12 in a weight ratio of 1:1:1.5, put them into a plastic bag and seal them for storage to obtain a feed additive for fattening. Example 6

[0049] The intramuscular fat deposition of 15-18 month old Holstein steers was verified by using the feed additive provided by the present invention for linear fattening. The test method and results are as follows: 1. Location: Science and Technology Innovation Experimental Base, Animal Husbandry Research Institute, Heilongjiang Academy of Agricultural Sciences, opposite No. 29, Zhengyi South Road, Nangang District, Harbin.

[0050] 2. Time: March 22, 2024 - July 8, 2024; 3. Cattle source: Heilongjiang Lvye Animal Husbandry Co., Ltd.; 4. Number of test heads: 32 heads, with an average weight of 603 kg, 16 months old, randomly divided into four groups, 8 heads in each group. 0%, 0.4%, 0.6%, 0.8% of the weight of the feed additive prepared in Example 1 were added to the diet, respectively, and the corresponding groups were recorded as Group 1, Group 2, Group 3, and Group 4, respectively. The specific situation is shown in Table 1. The nutrient concentration of the diet was determined by referring to the nutritional requirements of castrated dairy cattle and fattening cattle in "Japanese Feeding Standards • Beef Cattle (2008 Edition)" translated by Cao Binghai, P31-35.

[0051] Table 1 Group A group Group 2 Three groups Four groups variety Holstein Holstein Holstein Holstein gender Steer Steer Steer Steer Quantity Head 8 8 8 8 Initial weight kg 612.75±50.99 605.75±40.14 609.25±21.47 605.25±16.73 Age 488 488 488 488 Ratio of dietary addition % 0 0.4 0.6 0.8 5. Daily management: open-air loose-stall feeding, wearing electronic ear tags, feed intake equipment recording the daily feed intake of each head, free drinking water, stocking density of 12 square meters per head, feeding twice a day, once in the morning and once in the evening.

[0052] 6. Results 6.1 Effect of additives on weight gain performance of late-stage fattening Holstein steers (16-18 months old) in linear fattening. After 108 days of continuous feeding, the slaughter weight and average daily weight gain were measured and calculated. The results are shown in Table 2.

[0053] Table 2 Statistical results of cattle weight at slaughter and average daily weight gain in each group (x±s) Group A group Group 2 Three groups Four groups variety Holstein Holstein Holstein Holstein gender Steer Steer Steer Steer Quantity Head 8 8 8 8 Initial weight kg 612.75±50.99 605.75±40.14 609.25±21.47 605.25±16.73 Test days 108 108 108 108 Slaughter weight kg 739.25±56.89 717.13±40.80 725.00±29.55 722.9±55.59 Average daily weight gain 1.16±0.29 1.02±0.31 1.06±0.16 1.09±0.34 As shown in Table 2, among the four groups of cattle, one group had the highest average daily weight gain, but the difference in daily weight gain levels was not significant. This is related to the enhanced fat deposition ability of cattle in groups 2, 3, and 4. Enhanced fat deposition will lead to a decrease in daily weight gain.

[0054] 6.2 Effect of GAA Additive on Slaughter Performance of Holstein Steers According to the Japanese grading standard, the beef marbling grading results are shown in Table 3. The specific beef marbling pictures are shown in Figure 1-4 As shown, Figure 1 A set of marble rating images. Figure 2 These are the marbling rating images for the two groups. Figure 3 Here are three groups of marbling rating images. Figure 4Rating images for marbling in four groups.

[0055] Table 3 Rating results of backfat thickness and marbling of cattle in each group Group A group Group 2 Three groups Four groups Back fat thickness cm 2.29±0.47 2.16±0.66 2.01±0.41 2.13±0.23 Marbling Grade <![CDATA[2.5+0.53 b ]]> <![CDATA[2.63+0.52 b ]]> <![CDATA[3.38+0.52 a ]]> <![CDATA[3.25+0.48 a ]]> Level 2 accounts for 50.0%, Level 3 accounts for 50.0% Level 2 accounts for 37.5%, and Level 3 accounts for 62.5%. Level 3 accounts for 62.5%, and Level 4 accounts for 37.5% Level 3 accounts for 75.0%, and Level 4 accounts for 25.0% Note: The data in the table are mean ± standard deviation. Different lowercase letters on the shoulders of the same number in the table indicate significant differences (P<0.05). The higher the marbling grade, the higher the intramuscular fat content and the better the beef quality.

[0056] As shown in Table 3, the differences between Group 3 and Group 4 and Group 1 and Group 2 were significant (P < 0.05), and there was no significant difference between Group 3 and Group 4, with Group 3 having the highest marbling grade. It can be seen that the feed additive provided by the present invention has a significant effect on promoting intramuscular fat deposition in the late fattening period of Holstein steers raised in linear fattening, and can be used to improve the economic benefits of beef cattle. Example 7

[0057] 1. Location: Science and Technology Innovation Experimental Base, Animal Husbandry Research Institute, Heilongjiang Academy of Agricultural Sciences, opposite No. 29, Zhengyi South Road, Nangang District, Harbin.

[0058] 2. Time: July 22, 2024 - October 24, 2024; 3. Cattle source: Heilongjiang Lvye Animal Husbandry Co., Ltd.; 4. Number of test heads: 21 heads, with an average weight of 563.55 kg, 15 months old, randomly divided into three groups, 7 heads in each group. 0%, 0.6% of the feed additive prepared in Example 2 and 0.6% of the feed additive prepared in Example 4 were added to the diet, which were recorded as Group A, Group B and Group C, respectively. The specific situation is shown in Table 4. Dietary nutrient concentration: refer to "Japanese Feeding Standards • Beef Cattle (2008 Edition)" translated by Cao Binghai, P31-35, the nutritional requirements of castrated dairy cattle and fattening cattle.

[0059] Table 4 Group Group A Group B Group C variety Holstein Holstein Holstein gender Steer Steer Steer Quantity Head 7 7 7 Initial weight kg 561.12±38.67 562.10±38.59 567.43±37.41 Age 456.71±2.25 456.71±2.76 455.71±1.75 Ratio of dietary addition % 0 0.6% (Example 2 Feed Additive) 0.6% (Example 4 Feed Additive) 5. Daily management: open-air loose-stall feeding, wearing electronic ear tags, feed intake equipment recording the daily feed intake of each head, free drinking water, stocking density of 12 square meters per head, feeding twice a day, once in the morning and once in the evening.

[0060] 6. Results 6.1 Effect of additives on the weight gain performance of linear fattening Holstein steers in the late fattening period. After 94 consecutive days of feeding, the slaughter weight and average daily weight gain were measured and calculated. The results are shown in Table 5.

[0061] Table 5 Statistical results of cattle weight at slaughter and average daily weight gain in each group (x±s) Group Group A Group B Group C variety Holstein Holstein Holstein gender Steer Steer Steer Quantity Head 7 7 7 Initial weight kg 561.12±38.67 562.10±38.59 567.43±37.41 Test days 94 94 94 Slaughter weight kg 674.86±48.66 661.74±47.31 662.29±53.03 Average daily weight gain 1.21±0.16 1.06±0.2 1.01±0.21 As shown in Table 5, the average daily weight gain of group C was the highest among the three groups of cattle, but the difference in daily weight gain was not significant. This is related to the enhanced fat deposition ability of cattle. Enhanced fat deposition will lead to a decrease in daily weight gain.

[0062] 6.2 Effects of additives on slaughter performance of Holstein steers According to the Japanese grading standard, the beef marbling grading results are shown in Table 6 and Figure 5-7 As shown, Figure 5 This is a picture of the marble pattern rating for Group A. Figure 6 This is a picture of the marble pattern rating for Group B. Figure 7 This is a picture of the marbling rating of the Group C section.

[0063] Table 6 Rating results of backfat thickness and marbling of cattle in each group Group Group A Group B Group C Back fat thickness cm 2.1±0.23 1.95±0.17 2.0±0.24 Marbling Grade <![CDATA[2.57±0.53 a ]]> <![CDATA[3.29±0.49 b ]]> <![CDATA[3.57±0.79 b ]]> Level 2 accounts for 42.86%, and Level 3 accounts for 57.14%. Level 3 accounts for 71.43%, and Level 4 accounts for 28.57%. Level 3 accounts for 57.14%, Level 4 accounts for 28.57% and Level 5 accounts for 14.29% Note: The data in the table are mean ± standard deviation. Different lowercase letters in the same row of numbers indicate significant differences (P<0.05).

[0064] As can be seen from Table 6, among the three groups of cattle backfat thickness index, group A had the highest index and group B had the lowest index, but the difference was not significant, which was related to the effect of guanidine acetic acid; in terms of marbling rating, group C had the best marbling rating, followed by group B, and both groups B and C had significant differences from group A (P<0.05), while there was no significant difference between group B and group C. The feed additive provided by the present invention has a significant effect on promoting intramuscular fat deposition in 15-18 month old Holstein steers raised in linear fattening, and can be used to improve economic benefits.

[0065] The present invention has conducted the following experiments on some products configured in the early stage of the experiment, as follows: Comparative Example 1 Weigh out 1 part of GAA and 1 part of methionine by weight, put them into a stainless steel premixing tank and mix for 10 minutes, then put them into a plastic bag and seal them for storage.

[0066] Comparative Example 2 Weigh by weight: 1.2 parts of vitamin C, 1.8 parts of vitamin E, 2.0 parts of vitamin B complex, 2.5 parts of rumen-bypass fat and 2.5 parts of glucose, wherein the vitamin B complex is vitamin B1, vitamin B2 and vitamin B12 prepared in a weight ratio of 1:1:1.5. Put all the above raw materials into a stainless steel premixing tank, mix for 10 minutes, and put them into a plastic bag and seal them for storage.

[0067] Comparative Example 3 Weigh by weight: 1 part of GAA, 1 part of methionine, 1.2 parts of vitamin C, 1.8 parts of vitamin E, and 1.8 parts of vitamin B complex, wherein the vitamin B complex is prepared by vitamin B1, vitamin B2 and vitamin B12 in a weight ratio of 1:1:1.5. Put all the above raw materials into a stainless steel premixing tank, mix for 10 minutes, and put them into a plastic bag and seal them for storage.

[0068] Comparative Example 4 Weigh out by weight: 1 part of GAA, 1 part of methionine, 2.5 parts of rumen bypass fat and 2.5 parts of glucose, put all of the above raw materials into a stainless steel premixing tank, mix for 10 minutes, and then put them into a plastic bag and seal them for storage.

[0069] Twenty-four late-fattening Holstein steers (16 months old) with good body weight and health were selected and randomly divided into 4 groups, with 6 experimental heads in each group. The nutritional concentration of the daily diet was based on the nutritional requirements of castrated dairy and fattening cattle in "Japanese Feeding Standards•Beef Cattle (2008 Edition)" translated by Cao Binghai, P31-35. Daily management is open-air free-stall feeding, wearing electronic ear tags, and feed intake equipment records the daily feed intake of each head. Drinking water is free, and the stocking density is 12 square meters per head. Feed is given twice a day, once in the morning and once in the evening. After 100 days of feeding, the marbling rating results of each group of beef were statistically analyzed according to the Japanese rating standards. The results are shown in the figure. Figure 8-Figure 11 The marble grade was analyzed and compared with the experimental results of Group C in Example 7. The specific results are shown in Table 7. Figure 8 This is the marble rating picture of Comparative Example 1. Fig. 9 This is the marble rating picture of Comparative Example 2. Fig.10 This is the marble rating picture of Comparative Example 3. Fig.11 This is a marble rating picture of Comparative Example 4.

[0070] Table 7 Rating results of beef backfat thickness and marbling of each comparative example product Group Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Group C of Example 7 n 6 6 6 6 7 Back fat thickness cm <![CDATA[1.6±0.13 b ]]> <![CDATA[2.4±0.18 a ]]> <![CDATA[1.8±0.17 b ]]> <![CDATA[1.7±0.13 b ]]> <![CDATA[2.0±0.34 b ]]> Marbling Grade <![CDATA[2.50±0.55 cB ]]> <![CDATA[2.67±0.52 cB ]]> <![CDATA[3.17±0.41 bA ]]> <![CDATA[3.33±0.52 bA ]]> <![CDATA[3.57±0.79 aA ]]> Level 2 accounts for 50.00%, Level 3 accounts for 50.00% Level 2 accounts for 33.33% and level 3 accounts for 66.67%. Level 3 accounts for 83.33% and Level 4 accounts for 16.67% Level 3 accounts for 66.67% and Level 4 accounts for 33.33% Level 3 accounts for 57.14%, Level 4 accounts for 28.57% and Level 5 accounts for 14.29% Note: The data in the table are mean ± standard deviation; the same lowercase letters in the same row of numbers in the table indicate no significant difference ( P >0.05), lowercase letters indicate significant differences ( P <0.05, different capital letters indicate extremely significant differences ( P <0.01).

[0071] The results show that there is no significant difference in the effect of the combination of GAA and methionine in Comparative Example 1 and VC, VB and VE and substances for increasing fat synthesis, rumen fat and glucose, on intramuscular fat deposition in Comparative Example 2. On the basis of GAA and methionine, the addition of rumen fat and glucose can also significantly improve the efficiency of intramuscular fat deposition. When GAA and methionine are not added to the diet, subcutaneous fat deposition is large and the marbling of the formed beef is not good. When the additive is prepared with guanidinoacetic acid (GAA), methionine, vitamin C, vitamin E, vitamin B complex, rumen fat and glucose, marbled beef of grade 3.57 can be produced, which is significantly different from Comparative Examples 1 and 2, and significantly different from Comparative Examples 3 and 4. It can be seen that the feed additive prepared by guanidinoacetic acid (GAA), methionine, vitamin C, vitamin E, vitamin B complex, rumen fat and glucose in the present invention produces a synergistic gain effect and obtains a better muscle fat deposition effect.

Claims

1. A feed additive for increasing intramuscular fat deposition in late fattening Holstein steers, characterized in that: The invention is composed of 5-25 parts of guanidinoacetic acid, 10-25 parts of methionine, 5-20 parts of vitamin C, 5-25 parts of vitamin E, 10-25 parts of complex vitamin B, 10-35 parts of rumen bypass fat and 10-35 parts of glucose in parts by weight.

2. The feed additive according to claim 1, characterized in that The content of guanidine acetic acid is 8 to 20 parts, methionine is 10 to 22 parts, vitamin C is 8 to 20 parts, vitamin E is 10 to 20 parts, vitamin B complex is 10 to 18 parts, rumen fat is 15 to 30 parts, and glucose is 15 to 25 parts.

3. The feed additive according to claim 1, characterized in that The ingredients include 10 parts of guanidine acetic acid, 10 parts of methionine, 12 parts of vitamin C, 13 parts of vitamin E, 15 parts of vitamin B complex, 20 parts of rumen bypass fat and 20 parts of glucose.

4. The feed additive according to any one of claims 1 to 3, characterized in that Vitamin B complex is a mixture of vitamin B1, vitamin B2 and vitamin B12.

5. A feed containing the feed additive according to any one of claims 1 to 3.

6. The feed according to claim 5, characterized in that The content of the feed additive is 0.6-1.0% of the feed weight.

7. Use of the feed additive according to any one of claims 1 to 3 or the feed according to claim 5 or 6 in improving intramuscular fat deposition in late fattening stages of steers.

8. A method for increasing intramuscular fat deposition in steers, characterized in that: Add the feed additive according to any one of claims 1 to 3 to the diet or feed the feed according to claim 5 or 6 to the steer.

9. The method according to claim 8, characterized in that The feeding objects are straight-fattening Holstein steers at the age of 15 to 18 months.

10. The method according to claim 8 or 9, characterized in that The feed additive is fed for 90 to 120 days.

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