Traditional Chinese medicine composition for dispelling effects of alcohol and protecting liver and preparation method thereof

Abstract

The invention belongs to the technical field of medicines, and relates to a traditional Chinese medicine composition for dispelling effects of alcohol and protecting liver and a preparation method thereof. The traditional Chinese medicine composition is prepared from radix puerariae, flos puerariae, semen hoveniae, fructus crataegi, fructus mume and rhizoma imperatae. The preparation method comprises the following steps: adding 8-12 times of water into the medicinal materials in parts by weight, soaking and decocting, mixing decoction, and filtering to obtain filtrate; concentrating the filtrate under reduced pressure to obtain clear paste with the relative density of 1.08-1.14 (65 DEG C), refrigerating at 4-8 DEG C for 12 hours, centrifuging at 10000-15000 rpm, and taking supernate for later use; adding ethanol into the supernate until the alcohol content reaches 50-70%, refrigerating at 4-8 DEG C for 24 hours, filtering, recovering the ethanol from the filtrate until no alcohol smell exists, adding a proper amount of pure water, uniformly stirring, and then adding a proper amount of auxiliary materials; and adding purified water, centrifuging, filling supernate, and sterilizing at high temperature to obtain the traditional Chinese medicine composition. According to the invention, the traditional Chinese medicine compatibility is developed, the basic principles of dispelling the effects of alcohol, protecting the liver, reducing fever and causing diuresis are taken, the whole formula is properly matched, and the effects of dispelling the effects of alcohol, inducing urination, quenching thirst and arresting vomiting are achieved; functional tests prove that the beverage has obvious effects of dispelling the effects of alcohol and protecting the liver.

Description

Technical Field

[0001] The invention belongs to the technical field of medicines, and particularly relates to a traditional Chinese medicine composition for sobering up and protecting the liver and a preparation method thereof. Background Art

[0002] If a large amount of alcohol is consumed in a short period of time, it is easy to cause acute alcohol poisoning, resulting in headaches, dizziness, nausea, vomiting and other discomfort symptoms; long-term excessive drinking can cause chronic alcohol poisoning, alcoholic liver damage, myocardial fatigue, cirrhosis, renal failure and other pathological phenomena, and severe cases can also cause physiological dysfunction of the body, endangering human health; modern medical research shows that drinking a lot of alcohol has different degrees of damage to the human digestive system and endocrine system. Excessive drinking will reduce the liver's ability to purify blood and lead to an increase in toxins in the body. Among liver diseases, alcoholic liver disease is the main type of liver disease in my country, and it is showing an increasing trend year by year. The disease is a toxic liver disease caused by excessive drinking. Research data show that more than 85% of heavy drinkers have fatty liver, 15%-35% develop into alcoholic hepatitis, and 10%-25% will develop into severe diseases such as cirrhosis and liver cancer.

[0003] Alcohol is mainly absorbed in the digestive tract. After drinking, alcohol enters the stomach through the mouth, of which 30% is absorbed in the stomach and 60% is absorbed in the small intestine. The absorbed alcohol is metabolized in the liver, and the remaining 10% is excreted from the body through breathing, urine and sweat. Generally speaking, alcohol absorbed by the human body is oxidized into acetaldehyde by ADH (alcohol dehydrogenase). Acetaldehyde is then oxidized into acetic acid by ALDH (acetaldehyde dehydrogenase), and acetic acid is used as an energy source in the body.

[0004] Therefore, the sobering effect can be exerted in the following ways: blocking absorption by the stomach and intestines; accelerating decomposition in the stomach and intestines; accelerating decomposition in the liver; accelerating excretion through urine, sweat and other pathways; exerting a liver-protecting effect by combining with free radicals in the body and converting them into easily metabolizable acids, thereby accelerating the excretion of free radicals and playing a role in protecting the liver; or clearing out active free radicals contained in liver cells, thereby improving the liver's detoxification ability and playing a role in protecting the liver; or reducing the damage to the liver caused by free radicals produced by alcohol and maintaining the integrity of liver cell membranes; playing a role in protecting the liver.

[0005] There are many kinds of products for sobering up on the market, including Chinese herbal beverages, Chinese patent medicines, plant extracts, etc. The present invention is a natural Chinese herbal drink for sobering up and protecting the liver. All the raw materials in the formula, including kudzu root, kudzu flower, hovenia dulcis, hawthorn, ebony, and Imperata root, are national medicine and food homologous substances, which can effectively relieve various uncomfortable symptoms after drunkenness, including nausea, vomiting, etc., by sobering up and promoting urination. Summary of the invention

[0006] The purpose of the present invention is to give full play to the compatibility of traditional Chinese medicine, with the basic principles of sobering up and protecting the liver, clearing away heat and promoting diuresis. The whole formula is properly matched, and has the effects of sobering up and promoting urination, quenching thirst and stopping vomiting. Functional tests have confirmed that the present invention has obvious effects of sobering up and protecting the liver.

[0007] The invention provides a traditional Chinese medicine composition for sobering up and protecting the liver.

[0008] Another object of the present invention is to provide a method for preparing the Chinese medicine composition.

[0009] The technical solution adopted by the present invention is a traditional Chinese medicine composition for sobering up and protecting the liver, which is made of the following medicinal ingredients in parts by weight:

[0010] 6-24 parts of kudzu root, 4-16 parts of kudzu flower, 5-20 parts of Hovenia dulcis, 3-15 parts of hawthorn, 3-15 parts of ebony, and 5-20 parts of Imperata root.

[0011] The preparation method of the traditional Chinese medicine composition for sobering up and protecting the liver comprises the following steps, calculated by weight:

[0012] The first step is to add 8 to 12 times the amount of water to the above-mentioned parts by weight of Pueraria root, Pueraria flower, Hovenia dulcis, hawthorn, Prunus mume, and Imperata root, soak for 30 to 45 minutes, decoct for 1 to 3 times, each time for 1 to 2 hours, decoct together, filter through a 200-mesh sieve, and set aside the filtrate;

[0013] The second step is to concentrate the filtrate prepared in the step 1 under reduced pressure at -0.07Mpa to -0.09Mpa and 65 to 70°C to a clear paste with a relative density of 1.08 to 1.14, and measure the relative density at 65°C. After refrigerating at 4 to 8°C for 12 hours, centrifuge at a speed of 10000 to 15000 rpm, and take the supernatant for later use;

[0014] The third step is to add ethanol to the supernatant obtained in step 2 to make the alcohol content reach 50-70%, refrigerate at a temperature of 4-8° C. for 24 hours, filter, and reduce the ethanol in the filtrate under the conditions of -0.07Mpa to -0.09Mpa and 60-70° C. to recover the ethanol until there is no alcohol taste, add an appropriate amount of purified water, stir well, and set aside;

[0015] The fourth step is to add appropriate amount of auxiliary materials to the product obtained in the third step, stir to dissolve it, add purified water and centrifuge, take the supernatant, fill it, and sterilize it at high temperature to obtain the product.

[0016] Furthermore, the auxiliary material is one of citric acid, sodium citrate, acesulfame potassium, and aspartame, or a mixture of any of them.

[0017] The above-mentioned Chinese herbal medicine beverage can also be made into any other oral liquid preparation in pharmacy, such as oral solution, mixture, etc.;

[0018] The pharmacological effects of the components and their combinations are analyzed as follows:

[0019] Pueraria root, sweet and spicy in taste, cool in nature, enters the spleen and stomach meridians, its Qi is light and clear, it can raise yang and relieve muscles, promote fluid production, relieve restlessness and quench thirst, and is good at treating alcohol accumulation; Pueraria flower is sweet and cool in nature, enters the stomach meridian, can relieve alcohol and invigorate the spleen, relieve restlessness and quench thirst, and relieve nausea and vomiting; both have a strong alcohol-relieving effect; Hovenia dulcis fruit is sweet and sour in taste, neutral in nature, enters the heart and spleen meridians, quenches thirst and relieves restlessness, detoxifies alcohol, and promotes urination, which enhances the effect of the formula in relieving restlessness, quenching thirst and vomiting; Hawthorn fruit is sour and sweet in taste, slightly warm in nature, enters the spleen and stomach meridians, strengthens the spleen and eliminates dampness, and promotes blood circulation; Black plum contains citric acid, malic acid, succinic acid, sugars, sitosterol, vitamin C and other ingredients, which have the effects of relieving vomiting, relieving cough and quenching thirst. Imperata root is sweet in taste, cold and enters the lung, stomach and small intestine meridians. It has the functions of cooling blood, stopping bleeding, clearing heat and promoting diuresis, and can help Hovenia dulcis fruit to clear heat and promote diuresis;

[0020] Modern pharmacological research has confirmed that:

[0021] Pueraria root is a substance with medicinal and edible properties. It has the effects of relieving muscles and reducing fever, promoting body fluid and quenching thirst, raising yang and stopping diarrhea, promoting blood circulation and activating collaterals, and detoxifying alcohol. Pueraria root is rich in various chemical components, mainly isoflavones, triterpenes, saponins, alkaloids, coumarin compounds and polysaccharides, among which isoflavones and triterpenes are the main pharmacologically active ingredients. Modern pharmacological studies have shown that Pueraria root has various pharmacological effects such as improving cardiovascular and cerebrovascular diseases, anti-diabetes, anti-tumor, anti-virus, anti-inflammatory, anti-free radicals, preventing osteoporosis, protecting the liver, regulating immunity, detoxifying alcohol, and relieving fever.

[0022] Kudzu flower is a substance that is both a medicine and a food. It has the effect of sobering up and invigorating the spleen. It can be used to treat fever, thirst, loss of appetite, vomiting and acid reflux caused by alcohol. Kudzu flower is rich in various chemical components, mainly containing flavonoids, alkaloids, volatile oils, saponins, sterols and amino acids. Its main ingredients, saponins and isoflavones, have redox effects, which can accelerate the oxidation of alcohol, make ethanol lose its toxicity, shrink and protect the gastrointestinal mucosa, slow down the absorption of alcohol, and hinder the rapid and large-scale entry of alcohol into the blood circulation. Modern pharmacological studies have shown that kudzu flower has the effects of sobering up and protecting the liver, has antioxidant activity, and has a significant protective effect on myocardial cell hypoxia.

[0023] Hovenia dulcis is a substance that is both a medicine and a food. It has the effects of detoxifying alcohol, quenching thirst and relieving restlessness. Hovenia dulcis is rich in flavonoids, terpenes, and a variety of fatty acids. Modern pharmacological studies have shown that Hovenia dulcis can increase the activity of superoxide dismutase (SOD), alcohol dehydrogenase (ADH), acetaldehyde dehydrogenase (ALDH) and microsomal ethanol oxidase (EO) in the liver after drinking, achieving the activity of detoxifying alcohol and protecting the liver.

[0024] Hawthorn is a substance that is both a medicine and a food. It has the effects of strengthening the spleen and eliminating dampness, and promoting blood circulation. The main components of hawthorn are total flavonoids, organic acids, triterpene acids and proanthocyanidins. Modern pharmacological research has found that hawthorn applied to the digestive system can bidirectionally regulate gastrointestinal motility, promote the secretion of digestive enzymes, and protect the liver.

[0025] Black plum is a substance that can be used as both medicine and food. It has the effects of relieving vomiting, relieving cough and quenching thirst. It contains organic acids, amino acids, sterols, flavonoids, polysaccharides, terpenes and other ingredients. Modern pharmacological research has found that the terpenes, sterols, sugars and alkaloids in black plum have anti-tumor effects. Organic acids and volatile components have antioxidant and anti-inflammatory effects.

[0026] Imperata root is a substance with medicinal and edible properties, which has the effect of clearing heat and promoting diuresis. It contains triterpenes, lignans, sugars (sucrose, glucose, a small amount of fructose, xylose), lactones, sterols and organic acid compounds. Modern pharmacological studies have shown that Imperata root has diuretic, anti-inflammatory, hemostatic, antibacterial and immunomodulatory effects. The decoction of Imperata root is administered intragastrically, which has a diuretic effect on normal rabbits. The diuretic effect is most obvious after 5-10 days of administration. This effect may be related to the nervous system. If the peripheral nerves of the kidney are cut off, its diuretic effect will be lost. Some people also believe that the diuretic effect of Imperata root is related to the rich potassium salt it contains.

[0027] In order to demonstrate the effect of the present invention on sobering up and protecting the liver, the functional experimental report of the present invention is provided as follows:

[0028] 1. Experimental method: Healthy Kunming mice weighing about 20g were randomly divided into two groups, with 10 mice in each group. After fasting for 12 hours (but not water), they were weighed. The blank group was gavaged with 0.2mL of normal saline, and the experimental group was gavaged with about 0.2mL of beverage concentrate, with a dose equivalent to 600mL / (kg·mb). After an interval of 30 minutes, both groups were gavaged with 12.5mL / (kg·mb) of alcohol. The average drunkenness time and soberness time of each group of mice were observed, recorded and counted, and the timing was started from the time of gavage. Blood was collected from the mice's eye sockets every hour to measure the ethanol concentration in the blood.

[0029] 2. Data processing: SPSS24 software was used for statistical analysis. All experimental data were expressed as x±s, and the differences between different experimental groups were analyzed using Student-t test.

[0030] 3. Experimental results

[0031] 3.1 Healthy Kunming mice weighing about 20g were randomly divided into groups, with 10 mice in each group. After weighing, they were fasted (but not water-deprived) for 12 hours. After gavage according to regulations, the drunkenness time and soberness time were observed and recorded. The data are shown in Table 1.

[0032] Table 1 Drunkenness and soberness timetable (x±s, n=10)

[0033]

[0034] Note: * indicates that the comparison between this group and the blank group is significant, P < 0.05;

[0035] ** indicates that the comparison between this group and the blank group was P < 0.01, which is extremely significant.

[0036] From the above table, we can see that the average drunkenness time of the experimental group was significantly longer than that of the blank group (P<0.01). The average sobering time of the experimental group was significantly shorter than that of the blank group (P<0.05).

[0037] 3.2. Starting from the moment the mice were gavaged with alcohol, blood was collected from their eye sockets every hour to measure the ethanol concentration in their blood. The changes in the ethanol content in the mice's blood are shown in Table 2.

[0038] Table 2 Blood ethanol content (x±s, n=10)

[0039]

[0040] Note: * indicates that the comparison between this group and the blank group is significant, P < 0.05;

[0041] ** indicates that the comparison between this group and the blank group was P < 0.01, which is extremely significant.

[0042] From the information in the above table, it can be seen that the blood condition of mice in the experimental group was significantly better than that in the blank group. At 1, 4, and 5 hours, the blood ethanol content of the experimental group was significantly lower than that of the blank group (P < 0.05). At 2 and 6 hours, the blood ethanol content of the experimental group was extremely significantly lower than that of the blank group (P < 0.01).

[0043] Experimental results confirm that drinking an appropriate amount of hangover-detoxifying and liver-protecting beverage before drinking can effectively prolong the time of drunkenness and shorten the time of sobering up, thereby increasing the amount of alcohol consumed, preventing drunkenness, and sobering up, effectively avoiding or reducing acute liver damage caused by alcohol. DETAILED DESCRIPTION

[0044] Example 1

[0045] The Chinese medicine composition for sobering up and protecting the liver described in this embodiment is made of the following medicinal ingredients in parts by weight:

[0046] 6 parts of kudzu root, 4 parts of kudzu flower, 5 parts of hovenia dulcis, 3 parts of hawthorn, 3 parts of ebony, and 5 parts of imperata root.

[0047] The preparation method of the traditional Chinese medicine composition for sobering up and protecting the liver comprises the following steps, calculated by weight:

[0048] The first step is to add 8 times the amount of water to the above-mentioned parts by weight of Pueraria root, Pueraria flower, Hovenia dulcis, Hawthorn, Prunus mume, and Imperata root, soak for 30 minutes, boil for 1 hour, filter through a 200-mesh sieve, and set aside the filtrate;

[0049] The second step is to concentrate the filtrate prepared in step 1 under reduced pressure at -0.07 MPa and 65° C. to a clear paste with a relative density of 1.08, and measure the relative density at 65° C. After refrigeration at 4° C. for 12 hours, centrifuge at a speed of 10,000 rpm, and take the supernatant for later use;

[0050] The third step is to add ethanol to the supernatant obtained in step 2 to make the alcohol content reach 50%, refrigerate at 4°C for 24 hours, filter, and recover ethanol under reduced pressure at -0.07Mpa and 60°C until there is no alcohol taste, add an appropriate amount of purified water, stir well, and set aside;

[0051] The fourth step is to add appropriate amount of citric acid to the product obtained in the third step, stir to dissolve it, then add purified water and centrifuge, take the supernatant, fill it, and sterilize it at high temperature to obtain the product.

[0052] Example 2

[0053] The Chinese medicine composition for sobering up and protecting the liver described in this embodiment is made of the following medicinal ingredients in parts by weight:

[0054] 24 parts of kudzu roots, 16 parts of kudzu flowers, 20 parts of hovenia dulcis seeds, 15 parts of hawthorn, 15 parts of ebony plums, and 20 parts of imperata rhizomes.

[0055] The preparation method of the traditional Chinese medicine composition for sobering up and protecting the liver comprises the following steps, calculated by weight:

[0056] The first step is to add 12 times the amount of water to the above-mentioned parts by weight of Pueraria root, Pueraria flower, Hovenia dulcis, hawthorn, Prunus mume, and Imperata root, soak for 45 minutes, decoct for 3 times, each time for 2 hours, decoct together, filter through a 200-mesh sieve, and set aside the filtrate;

[0057] The second step is to concentrate the filtrate prepared in step 1 under reduced pressure at -0.09 MPa and 70°C to a clear paste with a relative density of 1.14, and measure the relative density at 65°C. After refrigerating at 8°C for 12 hours, centrifuge at 15,000 rpm and take the supernatant for later use;

[0058] The third step is to add ethanol to the supernatant obtained in step 2 to make the alcohol content reach 70%, refrigerate at 8°C for 24 hours, filter, and recover ethanol under reduced pressure at -0.09Mpa and 70°C until there is no alcohol taste, add an appropriate amount of purified water, stir well, and set aside;

[0059] The fourth step is to add an appropriate amount of sodium citrate to the product obtained in the third step, stir to dissolve it, then add purified water and centrifuge, take the supernatant, fill it, and sterilize it at high temperature to obtain the product.

[0060] Example 3

[0061] The Chinese medicine composition for sobering up and protecting the liver described in this embodiment is made of the following medicinal ingredients in parts by weight:

[0062] 15 parts of kudzu roots, 10 parts of kudzu flowers, 10 parts of hovenia dulcis seeds, 10 parts of hawthorn, 10 parts of ebony plums, and 10 parts of imperata rhizomes.

[0063] The preparation method of the traditional Chinese medicine composition for sobering up and protecting the liver comprises the following steps, calculated by weight:

[0064] The first step is to add 10 times the amount of water to the above-mentioned parts by weight of Pueraria root, Pueraria flower, Hovenia dulcis, hawthorn, Prunus mume, and Imperata root, soak for 10 minutes, decoct twice, each time for 1.5 hours, decoct together, filter through a 200-mesh sieve, and set aside the filtrate;

[0065] The second step is to concentrate the filtrate prepared in step 1 under reduced pressure at -0.08 MPa and 68° C. to a clear paste with a relative density of 1.1, and measure the relative density at 65° C. After refrigeration at 6° C. for 12 hours, centrifuge at 13,000 rpm, and take the supernatant for later use;

[0066] The third step is to add ethanol to the supernatant obtained in step 2 to make the alcohol content reach 60%, refrigerate at 6°C for 24 hours, filter, and recover ethanol under reduced pressure at -0.08Mpa and 65°C until there is no alcohol taste, add an appropriate amount of purified water, stir well, and set aside;

[0067] The fourth step is to add an appropriate amount of acesulfame potassium to the product obtained in the third step, stir to dissolve it, then add purified water and centrifuge, take the supernatant, fill it, and sterilize it at high temperature to obtain the product.

[0068] The auxiliary materials described in the above embodiments 1 to 3 may also be aspartame or a mixture of any of citric acid, sodium citrate and acesulfame potassium.

Claims

1. A Chinese medicine composition for sobering up and protecting the liver, characterized in that: Made from the following medicinal ingredients by weight: 6-24 parts of kudzu root, 4-16 parts of kudzu flower, 5-20 parts of Hovenia dulcis, 3-15 parts of hawthorn, 3-15 parts of ebony, and 5-20 parts of Imperata root.

2. A method for preparing the alcohol-relieving and liver-protecting Chinese medicine composition according to claim 1, characterized in that: The method comprises the following steps, calculated by weight: The first step is to add 8 to 12 times the amount of water to the above-mentioned parts by weight of Pueraria root, Pueraria flower, Hovenia dulcis, hawthorn, Prunus mume, and Imperata root, soak for 30 to 45 minutes, decoct for 1 to 3 times, each time for 1 to 2 hours, decoct together, filter through a 200-mesh sieve, and set aside the filtrate; The second step is to concentrate the filtrate prepared in the first step under reduced pressure at -0.07Mpa to -0.09Mpa and 65 to 70°C to a clear paste with a relative density of 1.08 to 1.14, and measure the relative density at 65°C. After refrigerating at 4 to 8°C for 12 hours, centrifuge at a speed of 10,000 to 15,000 rpm, and take the supernatant for later use; The third step is to add ethanol to the supernatant obtained in step 2 to make the alcohol content reach 50-70%, refrigerate at a temperature of 4-8° C. for 24 hours, filter, and reduce the ethanol in the filtrate under the conditions of -0.07Mpa to -0.09Mpa and 60-70° C. to recover the ethanol until there is no alcohol taste, add an appropriate amount of purified water, stir well, and set aside; The fourth step is to add appropriate amount of auxiliary materials to the product obtained in the third step, stir to dissolve it, add purified water and centrifuge, take the supernatant, fill it, and sterilize it at high temperature to obtain the product.

3. A method for preparing the alcohol-relieving and liver-protecting Chinese medicinal composition according to claim 2, characterized in that: The auxiliary material is one of citric acid, sodium citrate, acesulfame potassium and aspartame or a mixture of any of them.

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