Packing material detection method and application thereof

Abstract

The invention discloses a packing material detection method and application thereof, and belongs to the technical field of packing material quality detection. The method comprises the following steps: filling a sterile nutrient medium into a sterile packing material, sealing to obtain a sealed packing material, simulating a pollution source by using a spore suspension to pollute the sealed packing material, simulating a polluted environment by using a bacterial culture environment to culture spores on the surface of the sealed packing material, and simulating an invasion environment of bacteria. Detecting whether a nutrient medium in the packaging material is influenced by the simulated bacteria invasion environment, if bacteria are detected in the nutrient medium, indicating that bacterial spores invade the interior of the packaging material and the packaging material has poor protection capability on the bacteria, regarding the packaging material as an unqualified product, and if no bacteria are detected in the nutrient medium, regarding the packaging material as an unqualified product. The packaging material has good protection capability on bacteria, so that the packaging material can be regarded as a qualified product. According to the detection method, the detection result is accurate and reliable, the protection capability of the packaging material on various bacteria can be detected at the same time, and the detection efficiency is improved.

Description

Technical Field

[0001] The present invention relates to the technical field of packaging material quality detection, and in particular to a packaging material detection method and application thereof. Background Art

[0002] In the prior art, the detection of fungal invasion by packaging materials mainly relies on the following methods: (1) Fungal challenge test: by exposing the packaging material to a specific fungal environment, observe whether it can effectively block fungi. The disadvantages of this method are that the test conditions are complicated, the operation is difficult and the cost is high. (2) Gas permeability test: This method evaluates the gas permeability of the packaging material and indirectly infers the ability of fungi to invade. However, this method cannot directly measure fungal invasion and is often not targeted. (3) Physical penetration test: by applying a certain pressure, the physical strength of the packaging material is evaluated to determine its ability to protect against fungi. However, this method cannot simulate the actual fungal invasion process, and the test results may not be accurate enough.

[0003] The above existing methods generally have the following defects: the testing process is complicated, the operation is difficult, the testing cycle is long and the cost is high, and it is often difficult to accurately simulate the actual invasion process of fungi, thus affecting the accuracy and reliability of the test results.

[0004] In the prior art, the detection of bacterial invasion by packaging materials mainly relies on the following methods:

[0005] (1) Impact test: This method evaluates the durability of packaging materials by applying impact or pressure. Although impact testing can evaluate the mechanical properties of packaging materials, it cannot simulate the actual invasion process of bacterial spores through tiny defects or surface cracks.

[0006] (2) Biodegradation test: The protective performance of packaging materials is indirectly evaluated by detecting the degree of degradation of packaging materials under the action of microorganisms. The disadvantage of this method is that it cannot directly measure the invasion of spores, and the degradation rate is affected by many factors.

[0007] The main drawbacks of existing technical methods are the complexity of the test process, the limitation of operation, the time consumption and the insufficient simulation of the actual use environment. These methods may not fully evaluate the protective ability of the packaging material under the actual use conditions, thus affecting the accuracy and reliability of the test results. Summary of the invention

[0008] The main purpose of the present invention is to provide a packaging material detection method and application thereof, so as to solve the technical problem that it is difficult to evaluate the protective ability of packaging materials under actual use conditions in the prior art.

[0009] To achieve the above object, the present invention provides a method for detecting packaging materials, comprising the following steps:

[0010] Aseptically treating the packaging material to obtain a sterile packaging material;

[0011] Adding the aseptically treated nutrient matrix into the sterile packaging material under aseptic conditions and sealing the packaging material to obtain a sealed packaging material;

[0012] Immersing the sealing packaging material in a container containing a spore suspension and allowing the sealing packaging material to hit the bottom of the container;

[0013] The sealed packaging material after soaking is placed in an environment for culturing bacteria to obtain the packaging material to be tested;

[0014] Detect whether bacteria are present in the nutrient matrix of the packaging material to be detected, and judge whether the packaging material is qualified according to the detection result. If bacteria are detected, it is unqualified, and if no bacteria are detected, it is qualified.

[0015] In some embodiments of the present invention, the packaging material comprises a plastic packaging material.

[0016] In some embodiments of the present invention, the nutrient medium comprises a liquid culture medium.

[0017] In some embodiments of the present invention, the liquid culture medium comprises tryptic soy broth.

[0018] In some embodiments of the present invention, the spore suspension contains bacterial species, and the bacterial species includes Bacillus subtilis.

[0019] In some embodiments of the present invention, the frequency of the impact is once every 5 seconds; and / or, the time of the impact is 8 minutes to 10 minutes; and / or, the height of the impact is 15 cm to 20 cm.

[0020] In some embodiments of the present invention, the culture temperature of the environment for culturing bacteria is 37° C. to 38° C.; and / or the culture time is 48 h to 72 h.

[0021] In some embodiments of the present invention, in the step of detecting whether bacteria are present in the nutrient matrix in the packaging material to be detected, a microscope is used to observe whether bacteria are present in the nutrient matrix.

[0022] In some embodiments of the present invention, in the step of detecting whether bacteria are present in the nutrient matrix in the packaging material to be detected, the turbidity, OD value, and pH value of the nutrient matrix are recorded.

[0023] The present invention also provides an application of the above-mentioned packaging material detection method in the food field.

[0024] The beneficial effects that can be achieved by the present invention are:

[0025] The present invention loads a sterile nutrient matrix into a sterile packaging material, seals it to obtain a sealed packaging material, uses a spore suspension to simulate a pollution source to contaminate the sealed packaging material, uses a bacterial culture environment to simulate a pollution environment to culture spores on the surface of the sealed packaging material, and simulates the bacterial invasion environment well. Then, whether the nutrient matrix inside the packaging material is affected by the above simulated bacterial invasion environment is detected. If bacteria are detected in the nutrient matrix, it means that the bacterial spores have invaded the inside of the packaging material, the packaging material has a poor protection ability against bacteria, and it is regarded as an unqualified product. If no bacteria are detected in the nutrient matrix, it means that the bacterial spores have not invaded the inside of the packaging material, the packaging material has a good protection ability against bacteria, and it can be regarded as a qualified product.

[0026] The detection method of the present invention can demonstrate the actual invasion process of bacteria into the interior of the packaging material through tiny defects in the packaging material or surface cracks, can effectively reflect the protective ability of the packaging material against bacteria, is more representative than the single bacterial test in the prior art, can more realistically simulate the invasion process of bacterial spores, improve the accuracy and reliability of the detection results, and can simultaneously detect the protective ability of the packaging material against multiple bacteria, thereby improving the efficiency of detection. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the structures shown in these drawings without paying creative work.

[0028] Figure 1 The figure is a schematic flow chart of a method for detecting packaging materials according to an embodiment of the present invention.

[0029] Figure 2 Schematic diagrams showing that the packaging materials of Example 1 of the present invention and Comparative Example 1 are immersed in spore suspensions respectively.

[0030] Figure 3 Schematic diagram of comparison of culture medium in packaging materials of Example 1 of the present invention and Comparative Example 1.

[0031] Figure 4 This is a microscopic image of the culture medium in the packaging material of Comparative Example 1 of the present invention.

[0032] The realization of the purpose, functional features and advantages of the present invention will be further explained in conjunction with embodiments and with reference to the accompanying drawings. DETAILED DESCRIPTION

[0033] It should be understood that the specific embodiments described herein are only used to explain the present invention, and are not used to limit the present invention.

[0034] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0035] In the present invention, descriptions such as "first", "second", etc. are only used for descriptive purposes and cannot be understood as indicating or implying their relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include at least one of the features. In addition, the technical solutions between the various embodiments can be combined with each other, but they must be based on the ability of ordinary technicians in the field to implement them. When the combination of technical solutions is contradictory or cannot be implemented, it should be deemed that such a combination of technical solutions does not exist and is not within the scope of protection required by the present invention.

[0036] The present invention provides a method for identifying fungal spore invasion of packaging materials, comprising the following steps:

[0037] S10, performing aseptic treatment on the packaging material to obtain a sterile packaging material;

[0038] S20, adding the aseptically treated nutrient matrix into the sterile packaging material under aseptic conditions and sealing the packaging material to obtain a sealed packaging material;

[0039] S30, immersing the sealing packaging material into a container containing a spore suspension, and allowing the sealing packaging material to hit the bottom of the container;

[0040] S40, placing the soaked sealed packaging material in an environment for culturing bacteria to obtain a packaging material to be tested;

[0041] S50, detecting whether bacteria are present in the nutrient matrix of the packaging material to be detected, and judging whether the packaging material is qualified according to the detection result, if bacteria are detected, the packaging material is unqualified, and if no bacteria are detected, the packaging material is qualified.

[0042] The present invention loads a sterile nutrient matrix into a sterile packaging material, seals it to obtain a sealed packaging material, uses a spore suspension to simulate a pollution source to contaminate the sealed packaging material, uses a bacterial culture environment to simulate a pollution environment to culture spores on the surface of the sealed packaging material, and simulates the bacterial invasion environment well. Then, whether the nutrient matrix inside the packaging material is affected by the above simulated bacterial invasion environment is detected. If bacteria are detected in the nutrient matrix, it means that the bacterial spores have invaded the inside of the packaging material, the packaging material has a poor protection ability against bacteria, and it is regarded as an unqualified product. If no bacteria are detected in the nutrient matrix, it means that the bacterial spores have not invaded the inside of the packaging material, the packaging material has a good protection ability against bacteria, and it can be regarded as a qualified product.

[0043] The detection method of the present invention can demonstrate the actual invasion process of bacteria into the interior of the packaging material through tiny defects or surface cracks in the packaging material, and can effectively reflect the protective ability of the packaging material against bacteria. It is more representative than the single bacterial test in the prior art, and can more realistically simulate the invasion process of bacterial spores, thereby improving the accuracy and reliability of the detection results. Moreover, it can simultaneously detect the protective ability of the packaging material against multiple bacteria, thereby improving the efficiency of the detection.

[0044] The detection method of the present invention is applicable to a type of packaging material that can be sealed and is not easily soluble in aqueous solution. In some embodiments, the packaging material includes plastic packaging material, such as plastic bottle packaging material for bottled beverages. Therefore, the identification method of the present invention is applicable to the food field.

[0045] In some embodiments, food-grade plastic packaging materials with high barrier properties can be selected to ensure that they meet food safety standards. They can also have certain antibacterial properties, which can better reflect the packaging material's ability to protect against bacteria.

[0046] In step S10, the packaging material is aseptically treated, and the scope of the aseptic treatment includes the outside and inside of the packaging material to prevent the inside of the packaging material from being contaminated by bacteria before the nutrient matrix is ​​added, which may affect the accuracy of the detection.

[0047] In some embodiments, the packaging material can be sterilized using ultraviolet light or ethylene oxide.

[0048] In step S20, the nutrient matrix is ​​aseptically treated to prevent the sterile packaging material from being contaminated by the nutrient matrix containing bacteria and affecting the accuracy of the test. Similarly, adding the nutrient matrix to the sterile packaging material under aseptic conditions and sealing it can also prevent the nutrient matrix inside the packaging material from being contaminated and affecting the accuracy of the test.

[0049] In some embodiments, the nutrient matrix includes a liquid culture medium, which can provide the elements required for bacterial reproduction and promote bacterial growth, thereby better reflecting the protective ability of the packaging material against bacteria.

[0050] In some embodiments, the liquid culture medium includes tryptic soy broth (TSB), which is a commonly used nutrient-rich culture medium suitable for the growth of most bacteria, thereby enabling the simultaneous detection of the protective ability of the packaging material against multiple bacteria and improving the detection efficiency.

[0051] In the present invention, the content of the culture medium added to the packaging material can be determined according to the capacity of the packaging material, so that the packaging material with the added nutrient matrix is ​​easy to seal and not easy to cause the packaging material to rupture. In some embodiments, the content of the culture medium in the packaging material is 300ml to 500ml.

[0052] In step S30, the sealing packaging material is immersed in a container containing a spore suspension and is made to hit the bottom of the container. By hitting the bottom of the container, the outside of the sealing packaging material can be contaminated by the spores in the spore suspension, thereby improving the accuracy of detection.

[0053] In the present invention, the entire sealing packaging material can be immersed in a spore suspension to detect the overall protection ability of the packaging material against bacteria, or a partial area of ​​the packaging material can be immersed in a spore suspension to detect the protection ability of the area against bacteria. For example, the sealing part of the packaging material can be immersed in a spore suspension to detect the sealing effect of the packaging material.

[0054] In the present invention, the spore suspension contains bacterial spores, simulating the pollution source, and the type of bacterial spores can be selected according to the items to be detected.

[0055] In some embodiments, the spore-forming bacteria include Bacillus subtilis.

[0056] In some embodiments, the cultured spores can be suspended in sterile physiological saline to obtain a spore suspension. In the process of preparing the spore suspension, it is carried out in a sterile environment to avoid contamination by other bacteria and cause inaccurate test results.

[0057] In some embodiments, the concentration of the spore suspension is 10^4-6 CFU / mL.

[0058] In some embodiments, different types of bacterial spores can be added to sterile physiological saline, so that the protective effect of the packaging material on different types of bacteria can be identified simultaneously.

[0059] In some embodiments, the frequency of the impact is once every 5 seconds, which can not only meet the purpose of preventing the exterior of the sealed packaging material from being contaminated by spores in the spore suspension, but also is not likely to cause the packaging material to rupture and become contaminated.

[0060] In some embodiments, the impact time is 8 minutes to 10 minutes, which further ensures that the outside of the sealed packaging material is not contaminated by the spores in the spore suspension.

[0061] In some embodiments, the height of the impact is 15 cm to 20 cm.

[0062] In step S40, bacterial spores adhere to the surface of the sealed packaging material after immersion. In this step, the sealed packaging material is placed in an environment for culturing bacteria, which can promote the reproduction of bacterial spores and simulate an environment suitable for bacterial reproduction and growth, thereby better demonstrating the bacterial invasion process and improving the accuracy of detection. Moreover, culturing the bacterial spores before testing is more convincing in proving the quality of the packaging material.

[0063] In some embodiments, the culture temperature of the environment for culturing bacteria is 37° C. to 38° C., simulating an environment suitable for bacterial reproduction and growth.

[0064] In some embodiments, the culture time is 48 hours to 72 hours. When the culture period reaches a certain length, the bacteria can multiply to a certain number, which is not only easy to observe and identify, but also enhances the reliability and accuracy of the detection and is more convincing in proving the quality of the packaging material.

[0065] In step S50, the culture medium inside the packaging material that is contaminated and bred with bacteria is detected. If the packaging material has a poor ability to protect bacteria, the bacteria outside it will invade the internal nutrient matrix of the packaging material to contaminate it. If the packaging material has a good ability to protect bacteria, it can prevent the invasion of external bacteria, so the internal nutrient matrix will not be contaminated by bacteria and can maintain a sterile state. Therefore, the ability to protect bacteria of the packaging material can be reflected by monitoring whether there are bacteria in the nutrient matrix, so that it can be judged whether the packaging material is qualified. For example, if bacteria are detected in the nutrient matrix, it means that the packaging material has a poor ability to protect bacteria, which is an unqualified product. If bacteria are not detected in the nutrient matrix, it means that the packaging material has a good ability to protect bacteria, which can resist the invasion of bacteria and is a qualified product. In this step, the common detection means in this field can be selected to detect whether the nutrient matrix has bacterial growth.

[0066] In some embodiments, the nutrient medium can be taken out and observed with a microscope to see whether bacteria are present in the nutrient medium. This method is direct and highly accurate.

[0067] In some embodiments, the turbidity, OD value, and pH value of the nutrient medium can be detected to reflect whether bacteria have invaded and contaminated the nutrient medium.

[0068] Exemplarily, the turbidity test is as follows: the turbidity of the nutrient matrix is ​​measured using a turbidity meter. If the turbidity increases, it means that bacteria may be growing inside the packaging material.

[0069] Exemplarily, the OD value is detected as follows: the optical density (OD value) of the nutrient medium is measured using a spectrophotometer at a wavelength of 600 nm, and an increase in the OD value is generally associated with an increase in the bacterial concentration.

[0070] Exemplarily, pH detection is as follows: Use a pH meter to measure the pH of the nutrient medium. If the pH changes significantly, it may be due to the accumulation of bacterial metabolites.

[0071] In the process of detecting whether the nutrient matrix inside the packaging material contains bacteria, it is necessary to avoid contamination by bacteria outside the packaging material that may cause errors in the test results.

[0072] The packaging material detection method of the present invention can simulate the invasion process of bacteria, can better reflect the protective ability of packaging materials against bacteria, and can be applied to the food field, for example, to detect the protective ability of plastic packaging materials used for beverages against bacteria.

[0073] The technical solution of the present invention is further described in detail below in conjunction with specific embodiments. It should be understood that the following specific embodiments are only used to explain the present invention, and are not used to limit the present invention.

[0074] Example 1

[0075] The steps of the packaging material detection method of this embodiment are as follows:

[0076] S10: Select a PET bottle that meets the food safety standard as the packaging material to be tested, and use ultraviolet rays to sterilize the entire packaging material to obtain a sterile packaging material.

[0077] S20: Under sterile conditions, 300 ml of aseptically treated TSB culture medium is added into a sterile packaging material, and the packaging material is sealed to obtain a sealed packaging material.

[0078] S30: The cultured Bacillus subtilis spores are suspended in sterile physiological saline at a concentration of 1×10^4 CFU / mL to obtain a spore suspension, the sealed package of step S20 is immersed in a container containing the spore suspension, and the sealed package is impacted against the bottom of the container at a frequency of once every 5 seconds for a total of 10 minutes, and the impact height is 15 cm.

[0079] S40: Take out the sealed packaging material after soaking, transfer it to a 37°C culture environment through sterile operation, and culture it at 37°C for 48 hours to simulate the suitable conditions for bacterial growth. After culture, the packaging material to be tested is obtained.

[0080] S50: Check whether there are bacteria in the TSB culture medium in the packaging material.

[0081] Comparative Example 1

[0082] Comparative Example 1 identifies the same type of packaging material with reference to the identification method of Example 1, except that in step S20, the sterile packaging material is not completely sealed. The specific operation is: unscrewing the sealed lid of the packaging material and then slowly closing it to prevent liquid from leaking out.

[0083] like Figure 2 As shown, Figure 2 Schematic diagram of packaging materials containing TSB culture medium in Example 1 and Comparative Example 1 respectively immersed in spore suspension, Figure 1 The left side is the packaging material of Example 1. Figure 1 The packaging material of Comparative Example 1 is shown on the right side.

[0084] Performance Testing

[0085] The TSB culture medium of Example 1 and Comparative Example 1 was tested according to the following items.

[0086] (1) Turbidity test: Use a turbidity meter to measure the turbidity of the culture medium. If the turbidity increases, it means that bacteria may be growing inside the packaging material. At the same time, you can also observe with the naked eye whether the culture medium is turbid. The measurement results of the turbidity meter are shown in Table 1, and the results of the naked eye observation are shown in Table 2. Figure 3 ,like Figure 3 As shown, Figure 3 The left side is a schematic diagram of the culture medium of Example 1, and the right side is a schematic diagram of the culture medium of Comparative Example 1. It can be seen with the naked eye that the culture medium of Example 1 is transparent and clear, while the culture medium of Comparative Example 1 is relatively turbid. It can be preliminarily judged that the culture medium of Example 1 is not contaminated by bacteria, while bacteria appear in the culture medium of Comparative Example 1.

[0087] (2) OD value detection: The optical density (OD value) of the culture medium was measured using a spectrophotometer at a wavelength of 600 nm. An increase in OD value is generally associated with an increase in bacterial concentration. The detected results are shown in Table 1.

[0088] (3) pH value detection: Use a pH meter to measure the pH value of the culture medium. If the pH value changes significantly, it may be caused by the accumulation of bacterial metabolites. The results are shown in Table 1.

[0089] (4) Microscope observation: Take a small amount of culture medium sample and observe under a microscope whether there are bacteria, especially the characteristic morphology of Bacillus subtilis. Among them, no bacteria were observed in the culture medium of Example 1, while the microscopic image of the culture medium of Comparative Example 1 is as follows: Figure 4 As shown, from Figure 4 It can be found that bacteria appear in the culture medium of Comparative Example 1.

[0090] Table 1

[0091]

[0092]

[0093] Combination Figure 3 and Table 1:

[0094] About Example 1

[0095] Analysis of the measurement results of the initial value (0 hours):

[0096] OD value: The initial OD value at a wavelength of 600 nm was 0.04, which indicated that there was essentially no bacterial growth in the TSB medium and the OD value was close to the blank value.

[0097] pH value: The initial pH value was 6.75, and the culture medium was neutral, which was within the normal range of TSB culture medium.

[0098] Turbidity: The culture medium is transparent and not turbid, indicating that the packaging material was not contaminated during sealing.

[0099] Analysis of the 48-hour measurement results:

[0100] OD value: The initial OD value at a wavelength of 600 nm was 0.04, which indicated that there was essentially no bacterial growth in the TSB medium and the OD value was close to the blank value.

[0101] pH value: The pH value dropped to 5.8, which indicates that the bacterial metabolic activity was strong and the acidic metabolites may have increased.

[0102] Turbidity: The culture medium is noticeably turbid, further confirming the high growth of bacteria.

[0103] About Comparative Example 1

[0104] Analysis of the measurement results of the initial value (0 hours):

[0105] OD value: The initial OD value at a wavelength of 600 nm was 0.05, which indicated that there was basically no bacterial growth in the TSB medium and the OD value was close to the blank value.

[0106] pH value: The initial pH value was 6.8, and the culture medium was neutral, which was within the normal range of TSB culture medium.

[0107] Turbidity: The culture medium is transparent and not turbid, indicating that the packaging material was not contaminated during sealing.

[0108] Analysis of the 48-hour measurement results:

[0109] OD value: The OD value significantly increased to 0.8, indicating that the number of bacteria in the packaging material increased significantly and the bacteria multiplied in the TSB medium.

[0110] pH value: The pH value dropped to 5.8, which indicates that the bacterial metabolic activity was strong and the acidic metabolites may have increased.

[0111] Turbidity: The culture medium is noticeably turbid, further confirming the high growth of bacteria.

[0112] It can be seen that the detection method of the present invention can demonstrate the actual invasion process of bacteria into the interior of the packaging material through tiny defects in the packaging material or surface cracks, can effectively reflect the protective ability of the packaging material against bacteria, is more representative than the single bacterial test in the prior art, can more realistically simulate the invasion process of bacterial spores, improve the accuracy and reliability of the detection results, and can simultaneously detect the protective ability of the packaging material against multiple bacteria, thereby improving the efficiency of detection.

[0113] The above are only preferred embodiments of the present invention, and are not intended to limit the patent scope of the present invention. Any equivalent structure or equivalent process transformation made using the contents of the present invention specification and drawings, or directly or indirectly applied in other related technical fields, are also included in the patent protection scope of the present invention.

Claims

1. A method for detecting packaging materials, characterized in that: The following steps are involved: Aseptically treating the packaging material to obtain a sterile packaging material; Adding the aseptically processed nutrient matrix into the sterile packaging material under aseptic conditions and sealing the packaging material to obtain a sealed packaging material; Immersing the sealing packaging material in a container containing a spore suspension and allowing the sealing packaging material to hit the bottom of the container; The sealed packaging material after soaking is placed in an environment for culturing bacteria to obtain the packaging material to be tested; Detect whether bacteria are present in the nutrient matrix of the packaging material to be detected, and judge whether the packaging material is qualified according to the detection result. If bacteria are detected, it is unqualified, and if no bacteria are detected, it is qualified.

2. The method for detecting packaging materials according to claim 1, characterized in that: The packaging material includes plastic packaging material.

3. The method for detecting packaging materials according to claim 1, characterized in that: The nutrient medium includes a liquid culture medium.

4. The method for detecting packaging materials according to claim 1, characterized in that: The liquid culture medium includes tryptic soy liquid culture medium.

5. The method for detecting packaging materials according to claim 1, characterized in that: The spore suspension contains bacterial species, and the bacterial species include Bacillus subtilis.

6. The method for detecting packaging materials according to claim 1, characterized in that: The frequency of the impact is once every 5 seconds; and / or, the time of the impact is 8 minutes to 10 minutes; and / or, the height of the impact is 15 cm to 20 cm.

7. The method for detecting packaging materials according to claim 1, characterized in that: The culture temperature of the environment for culturing bacteria is 37° C. to 38° C.; and / or the culture time is 48 h to 72 h.

8. The method for detecting packaging materials according to claim 1, characterized in that: In the step of detecting whether bacteria exist in the nutrient matrix in the packaging material to be detected, a microscope is used to observe whether bacteria exist in the nutrient matrix.

9. The method for detecting packaging materials according to claim 1, characterized in that: In the step of detecting whether bacteria are present in the nutrient matrix in the packaging material to be detected, the turbidity, OD value, and pH value of the nutrient matrix are recorded.

10. Use of the packaging material detection method according to any one of claims 1 to 9 in the food field.

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