Chemiluminescence detection kit for thyroglobulin and detection method thereof

Abstract

The invention relates to the related technical field of thyroglobulin detection, in particular to a thyroglobulin chemiluminescence detection kit and a detection method thereof.The thyroglobulin chemiluminescence detection kit comprises a kit body structure, the kit body structure is composed of an upper shell and a lower shell which are detachably connected in a sealed connection mode, and a dripping detection hole is formed in the upper shell; the thickening pad and the analysis structure are located in the box body structure, and a gap exists between the thickening pad and the analysis structure; the blocking assembly is installed on the upper shell, a connecting pad for conducting the thickening pad and the analysis structure is installed at the end, arranged in the box body structure, of the blocking assembly, and when the blocking assembly is controlled to release the blocking state of the drop measuring hole, the blocking assembly is controlled to release the blocking state of the drop measuring hole. When the kit is used, the connecting pad can be driven to move downwards to abut against the thickening pad and the analysis structure, and it is guaranteed that the interior of the kit body structure is always kept closed before the kit is started, so that external substances cannot enter the kit body structure through the dropping detection hole.

Description

Technical Field

[0001] The present invention relates to the technical field related to thyroglobulin detection, and in particular to a chemiluminescence detection kit for thyroglobulin and a detection method thereof. Background Art

[0002] Thyroglobulin (Tg) is a macromolecular glycoprotein secreted by thyroid follicular epithelial cells. Most of it is synthesized by thyroid cells and released into the residual cavity of thyroid follicles. It is the main storage form in the thyroid gland and is responsible for storing thyroxine (the precursor of thyroid hormone) and some other iodides.

[0003] The detection methods of thyroglobulin (Tg) mainly include chemiluminescent immunoassay, radioimmunoassay and enzyme-linked immunosorbent assay. Among them, chemiluminescent immunoassay uses anti-thyroglobulin antibodies as measurement markers to determine the Tg concentration in serum through chemiluminescent reactions. Roche Cobas E601 and Siemens Centaur XP are two commonly used chemiluminescent detection systems that can perform high-precision determination of TGAb and have high accuracy, sensitivity and specificity.

[0004] The droplet hole on the chemiluminescent detection kit for thyroglobulin currently sold on the market is basically in an open state, and the thickened pad in the box is always kept in contact with the gold pad. Before the formal detection process, if irrelevant external substances, such as water droplets, are accidentally dropped into the box, the coating on the test paper in the box will be diluted, reducing the accuracy of the detection and even causing the kit to fail. Summary of the invention

[0005] The object of the present invention is to provide a chemiluminescent detection kit for thyroglobulin to solve the problems raised in the above background technology.

[0006] To achieve the above object, the present invention provides the following technical solutions: A chemiluminescent detection kit for thyroglobulin comprises a box structure, wherein the box structure is composed of a detachably connected upper shell and a sealed lower shell, and a drop detection hole is arranged on the upper shell; It also includes a thickening pad and an analysis structure located inside the box structure, with a gap between the thickening pad and the analysis structure; A plugging component is installed on the upper shell. A connecting pad that connects the thickening pad and the analysis structure is installed on one end of the plugging component arranged inside the box structure. The plugging component is controlled to release the blocking state of the drip measuring hole while driving the connecting pad to move downward to abut against the thickening pad and the analysis structure.

[0007] The chemiluminescent detection kit for thyroglobulin as described above: a plurality of upper grooves are arranged on the inner wall of the upper shell, and lower convex columns adapted to the upper grooves are formed on the inner wall of the lower shell, and the lower convex columns can be inserted into the upper grooves.

[0008] The chemiluminescent detection kit for thyroglobulin as described above: the analysis structure includes a gold pad, an NC membrane and a water absorbent pad, there is a gap between one end of the gold pad and the thickened pad, the other end overlaps with one end of the NC membrane, and the other end of the NC membrane overlaps with the water absorbent pad.

[0009] The chemiluminescent detection kit for thyroglobulin as described above: a plurality of groups of limit blocks are symmetrically arranged inside the lower shell, and the limit blocks are respectively in contact with the thickening pad and the gold pad.

[0010] The chemiluminescent detection kit for thyroglobulin as described above: an observation port is formed on the upper shell, the observation port is located directly above the NC membrane, and a transparent lens is sealed and installed at the observation port.

[0011] The chemiluminescent detection kit for thyroglobulin as described above: the upper shell is provided with a mounting hole penetrating the upper shell, and a limited position sliding groove is formed on the inner wall of the mounting hole.

[0012] The chemiluminescent detection kit for thyroglobulin as described above: the blocking component comprises a pressing piece arranged axially along the mounting hole, a protrusion adapted to the limiting slide groove is formed on the pressing piece, one end of the pressing piece extending into the mounting hole is slidably connected to a connecting sleeve rotatably mounted inside the upper shell, and an extrusion piece slidably connected to the upper shell is provided at the bottom of the connecting sleeve; It also includes a spring, which is arranged in the connecting sleeve, one end of the spring abuts against the pressing piece, and the other end abuts against the inner bottom of the connecting sleeve.

[0013] The chemiluminescent detection kit for thyroglobulin as described above: the connecting sleeve is provided with an abutment plate in an arc-shaped structure, and a through hole adapted to the droplet measuring hole is formed on the abutment plate.

[0014] The chemiluminescent detection kit for thyroglobulin as described above: the extrusion member includes a pressure plate, which is slidably arranged in the upper shell body through a support rod, and a sleeve is arranged axially along the connecting sleeve at the center position of the pressure plate, and the sleeve is slidably connected to the bottom of the connecting sleeve.

[0015] A chemiluminescent detection method for thyroglobulin, using the chemiluminescent detection kit for thyroglobulin as described in any one of the above, comprising the following steps; Step 1: Before blood collection, take out the test kit and clean and disinfect the collection site; Step 2: Take out the reagent kit, unpack the packaging on the surface of the reagent kit, and lay the reagent kit flat. At this time, the droplet hole is in a blocked state to prevent external water from entering the box structure through the droplet hole before collection; Step three: by pressing and rotating the blocking structure, the dripping hole can be controlled to release the blocking state, and the connecting pad can be driven down to abut against the thickening pad and the analysis structure. After the collected blood falls onto the thickening pad through the dripping hole, the blood to be tested is absorbed into the analysis structure for testing under the action of the connecting pad.

[0016] Compared with the prior art, the invention has the following beneficial effects: before the reagent kit is activated, the interior of the box structure is always kept closed, so that foreign substances cannot enter the box structure through the dripping hole; The separate setting of the thickening pad and the gold pad ensures that even if the sealing performance of the dripping hole is weakened, external substances falling through the dripping hole will only drip onto the thickening pad. Due to the gap between the thickening pad and the gold pad, the substances cannot be transmitted to the gold pad and the NC membrane, preventing the detection substances on the NC membrane from being diluted. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 Schematic diagram of the structure of the chemiluminescent detection kit for thyroglobulin.

[0018] Figure 2 This is a schematic diagram of the structure of the upper shell and the lower shell in the chemiluminescent detection kit for thyroglobulin.

[0019] Figure 3 This is a cross-sectional schematic diagram of the box structure in the chemiluminescent detection kit for thyroglobulin.

[0020] Figure 4 This is a schematic diagram of the structure of the upper shell in the chemiluminescent detection kit for thyroglobulin.

[0021] Figure 5 This is a schematic diagram of the structure of the upper shell of the chemiluminescent detection kit for thyroglobulin from another angle.

[0022] Figure 6 This is a schematic diagram of the structure of the upper shell and the blocking component in the chemiluminescent detection kit for thyroglobulin.

[0023] Figure 7 Chemiluminescent detection kit for thyroglobulin Figure 6 A is an enlarged schematic diagram of the structure.

[0024] Figure 8Schematic diagram of the decomposition of the blocking component in the chemiluminescent detection kit for thyroglobulin.

[0025] Fig. 9 This is a schematic diagram of the structure of the extruded parts in the chemiluminescent detection kit for thyroglobulin.

[0026] Fig.10 This is a schematic diagram of the structure of the pressing piece and the connecting sleeve in the chemiluminescent detection kit for thyroglobulin.

[0027] Fig.11 This is a schematic structural diagram of the upper shell in the chemiluminescent detection kit for thyroglobulin from another angle.

[0028] Fig.12 Chemiluminescent detection kit for thyroglobulin Fig.11 The enlarged structural diagram at B on the figure is shown.

[0029] In the figure: 1. upper shell; 101. observation port; 102. mounting hole; 1021. limit slide groove; 103. drip measuring hole; 104. upper groove; 105. arc slide groove; 2. lower shell; 201. lower boss; 202. limit block; 3. sealing strip; 4. thickening pad; 5. gold pad; 6. NC film; 7. water-absorbing pad; 8. support rod; 801. limit groove; 9. pressure plate; 901. push rod; 9011. limit block; 10. sleeve; 1001. first ball; 11. pressing piece; 1101. protrusion; 1102. second ball; 12. spring; 13. connecting sleeve; 1301. vertical groove; 1302. threaded groove; 14. abutment plate; 1401. through hole; 1402. rotating block. DETAILED DESCRIPTION

[0030] Various exemplary embodiments, features and aspects of the present application will be described in detail below with reference to the accompanying drawings. The same reference numerals in the accompanying drawings represent elements with the same or similar functions. Although various aspects of the embodiments are shown in the accompanying drawings, the drawings are not necessarily drawn to scale unless otherwise specified.

[0031] The word “exemplary” is used exclusively herein to mean “serving as an example, example, or illustration.” Any embodiment described herein as “exemplary” is not necessarily to be construed as preferred or advantageous over other embodiments.

[0032] In addition, in order to better illustrate the present application, numerous specific details are provided in the specific embodiments below. It should be understood by those skilled in the art that the present application can also be implemented without certain specific details. In some examples, methods, means, and elements well known to those skilled in the art are not described in detail in order to highlight the subject matter of the present application.

[0033] See also Figure 1 to Figure 12In an embodiment of the present invention, a chemiluminescent detection kit for thyroglobulin includes a box structure, wherein the box structure is composed of a detachably connected upper shell 1 and a lower shell 2 that are sealed together, wherein the upper shell 1 and the lower shell 2 are made of plastic material; The upper shell 1 is provided with a dripping hole 103; It also includes a thickening pad 4 and an analysis structure located inside the box structure, and there is a gap between the thickening pad 4 and the analysis structure; The plugging component is installed on the upper shell 1. The plugging component is arranged on one end inside the box body structure and is equipped with a connecting pad that connects the thickening pad 4 and the analysis structure. While controlling the plugging component to release the blocking state of the drip measuring hole 103, it can drive the connecting pad to move downward to abut against the thickening pad 4 and the analysis structure.

[0034] In detail, in this embodiment, the chemiluminescent detection kit for thyroglobulin described in the present invention is adopted. During detection, the blocking component is controlled to move and rotate relative to the upper shell 1. When the blocking component moves relative to the upper shell 1, it deforms to reserve elastic potential energy. When the blocking component rotates relative to the upper shell 1, the blocking state of the dripping hole 103 is released. At the same time, the connecting pad can be driven to move down to abut against the thickening pad 4 and the analysis structure, so that the serum can fall onto the thickening pad 4 through the dripping hole 103. Under the action of the connecting pad, the serum can be transported to the analysis structure for detection, ensuring that before the kit is activated, the interior of the box structure is always kept closed, so that foreign substances cannot enter the box structure through the dripping hole 103.

[0035] At the same time, the separate setting of the thickening pad 4 and the analysis structure ensures that even if the sealing performance of the dripping hole 103 is weakened, external substances that fall through the dripping hole 103 will only drip onto the thickening pad 4. Due to the gap between the thickening pad 4 and the analysis structure, the substances cannot be transmitted to the analysis structure, preventing the detection substances on the analysis structure from being diluted, thereby ensuring the overall integrity of the analysis structure before detection.

[0036] As a further solution of the present invention, please refer to Figure 2 A plurality of upper grooves 104 are provided on the inner wall of the upper shell 1 , and a lower boss 201 adapted to the upper grooves 104 is formed on the inner wall of the lower shell 2 , and the lower boss 201 can be inserted into the upper grooves 104 .

[0037] To elaborate, when installing the upper shell 1 and the lower shell 2, align the lower boss 201 on the lower shell 2 with the upper groove 104 on the upper shell 1, and control the lower boss 201 to insert into the upper groove 104 to achieve a detachable connection between the upper shell 1 and the lower shell 2, wherein a sealing strip 3 adapted to the upper shell 1 and the lower shell 2 is sleeved at the connection between the upper shell 1 and the lower shell 2 to prevent the occurrence of gaps that affect the sealing of the upper shell 1 and the lower shell 2.

[0038] The analysis structure includes a gold pad 5 , a NC membrane 6 and a water absorbent pad 7 . There is a gap between one end of the gold pad 5 and the thickening pad 4 , and the other end overlaps with one end of the NC membrane 6 . The other end of the NC membrane 6 overlaps with the water absorbent pad 7 .

[0039] The NC film 6 is provided with a C line (control line) and a T line (test line) along its width direction.

[0040] See also Figure 2 A plurality of groups of limit blocks 202 are symmetrically arranged inside the lower shell 2 , and the limit blocks 202 are respectively in contact with the thickening pad 4 and the gold pad 5 .

[0041] The setting of the limit block 202 mainly plays a limiting role on the thickening pad 4 and the gold pad 5. The thickening pad 4 and the gold pad 5 are fixed to the inner bottom of the lower shell 2, but hot melt adhesive is generally used to bond them during connection. When the box structure is vibrated or affected by the temperature, the thickening pad 4 and the gold pad 5 are easy to separate from the lower shell 2. The existence of the limit block 202 can prevent the position of the thickening pad 4 and the gold pad 5 from shifting, and avoid direct contact between the thickening pad 4 and the gold pad 5.

[0042] As a further solution of the present invention, please refer to Figure 2 An observation port 101 is formed on the upper shell 1 , and the observation port 101 is located directly above the NC film 6 . A transparent lens is sealed and installed at the observation port 101 .

[0043] The upper shell 1 is provided with a mounting hole 102 penetrating the upper shell 1 , and at least one set of limiting sliding grooves 1021 is formed on the inner wall of the mounting hole 102 .

[0044] The limiting sliding groove 1021 is divided into a first strip groove, a second arc groove, and a third strip groove.

[0045] As a further solution of the present invention, please refer to Figure 6 and Figure 7The blocking assembly includes a pressing member 11 axially arranged along the mounting hole 102, and a protrusion 1101 adapted to the limiting slide groove 1021 is formed on the pressing member 11. One end of the pressing member 11 extending into the mounting hole 102 is slidably connected to a connecting sleeve 13 rotatably mounted inside the upper shell 1, and an extrusion member slidably connected to the upper shell 1 is provided at the bottom of the connecting sleeve 13; The connecting sleeve 13 further comprises a spring 12 , which is disposed in the connecting sleeve 13 , one end of the spring 12 abuts against the pressing member 11 , and the other end abuts against the inner bottom of the connecting sleeve 13 .

[0046] To elaborate, when the pressing piece 11 is pressed, the pressing piece 11 slides relative to the inside of the mounting hole 102 and drives the protrusion 1101 to move synchronously. At this time, the protrusion 1101 moves in the direction of the first strip groove. During the downward movement of the pressing piece 11, the spring 12 is squeezed, and the spring 12 is compressed to reserve elastic potential energy. When the protrusion 1101 moves to the end of the first strip groove, the pressing piece 11 cannot move downward under the restriction of the first strip groove. At this time, the spring 12 is completely compressed. During the movement of the protrusion 1101 in the direction of the first strip groove, the drip measuring hole 103 always remains in a blocked state, and the thickening pad 4 and the gold pad 5 cannot contact each other.

[0047] In contrast, the droplet holes on most test kits currently produced on the market are in an open state, and the thickened pad 4 and the gold pad 5 are in direct contact. Therefore, before the test kit is tested, once the external water sample is accidentally dropped into the droplet hole, the liquid will first be absorbed by the gold pad 5. Under the action of the absorbent pad 7, the liquid flows along the gold pad 5 to the NC membrane 6, so that the gold pad 5, NC membrane 6, and absorbent pad 7 are soaked with liquid, so that the detection substance on the NC membrane 6 is dispersed onto the gold pad 5 and the absorbent pad 7. Even if the water on the gold pad 5, NC membrane 6, and absorbent pad 7 is evaporated later, the detection substance that has been dispersed onto the gold pad 5 and the absorbent pad 7 cannot return to the NC membrane 6, so that during the formal test, the serum cannot fully react with the detection substance on the NC membrane 6, resulting in errors in the test results or no response to the test.

[0048] Preferably, a second ball 1102 is movably provided on one end of the pressing piece 11 inserted into the connecting sleeve 13, and a vertical groove 1301 is formed on the inner wall of the connecting sleeve 13, which slides with the second ball 1102, wherein the length of the vertical groove 1301 is equal to the length of the first strip groove, so that when the protrusion 1101 moves to the end of the first strip groove, the second ball 1102 moves to the end of the vertical groove 1301.

[0049] After the spring 12 is fully compressed, the pressing piece 11 is controlled to rotate relative to the mounting hole 102. With the cooperation of the second ball 1102 and the vertical groove 1301, the extrusion connecting sleeve 13 rotates synchronously with the pressing piece 11. When the pressing piece 11 rotates, the protrusion 1101 rotates along the direction of the second arc groove. The rotation direction of the pressing piece 11 must be the same as the direction of the second arc groove so that the pressing piece 11 can rotate smoothly relative to the mounting hole 102.

[0050] As a further solution of the present invention, please refer to Figure 8 The connecting sleeve 13 is provided with an abutting plate 14 in an arc-shaped structure, and the abutting plate 14 is formed with a through hole 1401 adapted to the drip detection hole 103 .

[0051] When the connecting sleeve 13 rotates, the abutment plate 14 is driven to rotate synchronously. When the protrusion 1101 rotates to the end of the second arc groove, the through hole 1401 on the abutment plate 14 is completely aligned with the dripping hole 103 to release the blocking state of the dripping hole 103, so that the collected serum can enter the box structure through the dripping hole 103 for detection.

[0052] Preferably, a rotating block 1402 is provided on one end of the abutment plate 14 away from the connecting sleeve 13, and an arc-shaped slide groove 105 is formed on the inner wall of the upper shell 1 to rotate with the rotating block 1402. When the abutment plate 14 rotates under the drive of the connecting sleeve 13, the rotating block 1402 rotates in a directional manner along the direction of the arc-shaped slide groove 105 to realize the rotational connection between the abutment plate 14 and the inner wall of the upper shell 1.

[0053] As a further solution of the present invention, please refer to Fig. 9 The extrusion member includes a pressing plate 9, which is slidably arranged in the upper shell 1 through a supporting rod 8. A sleeve 10 is arranged axially along the connecting sleeve 13 at the center position of the pressing plate 9, and the sleeve 10 is slidably connected to the bottom of the connecting sleeve 13.

[0054] Preferably, a first ball 1001 is movably provided on the inner wall of the sleeve 10, a thread groove 1302 slidably matched with the first ball 1001 is formed at the bottom of the connecting sleeve 13, and the thread direction of the thread groove 1302 is the same as the direction of the second arc groove.

[0055] Further, when the above-mentioned connecting sleeve 13 rotates, the sleeve 10 is squeezed under the cooperation of the first ball 1001 and the threaded groove 1302, wherein push rods 901 are arranged on both sides of the pressing plate 9, and at least one group of limit blocks 9011 is arranged on the push rod 901, and a support rod 8 is fixedly installed on the inner top of the upper shell 1, and a limit groove 801 is formed on the support rod 8, which slides with the limit block 9011. Under the limiting action of the limit groove 801 and the limit block 9011, the sliding connection between the pressing plate 9 and the support rod 8 is realized, so that the sleeve 10 is squeezed by the connecting sleeve 13 and moves downward, so as to drive the sample pad on the pressing plate 9 to move downward, and one end of the sample pad overlaps with the thickening pad 4, and the other end overlaps with the gold pad 5, so that the serum can enter the box structure through the droplet hole during detection, and under the action of the sample pad, it is ensured that the NC membrane 6 can be soaked, thereby displaying the test result.

[0056] In general, when the protrusion 1101 slides to the end of the second arc-shaped groove, the drip measuring hole 103 has been unblocked, and the two ends of the connecting plate overlap with the thickening pad 4 and the gold pad 5 respectively. At this time, the pressing of the pressing piece 11 is stopped, and under the elastic action of the spring 12, the protrusion 1101 is slid into the third strip groove, and under the restriction of the end of the third strip groove, the position of the pressing piece 11 is locked, so that the detection work after serum collection can be carried out smoothly.

[0057] A chemiluminescent detection method for thyroglobulin, using the chemiluminescent detection kit for thyroglobulin as described in any one of the above, comprising the following steps; Step 1: Before blood collection, take out the test kit and clean and disinfect the collection site; Step 2: Take out the reagent kit, unpack the packaging on the surface of the reagent kit, and lay the reagent kit flat. At this time, the droplet hole 103 is in a blocked state to prevent external water from entering the box structure through the droplet hole 103 before collection; Step three: by pressing and rotating the blocking structure, the dripping hole 103 can be controlled to release the blocking state, and the connecting pad can be driven to move down to abut against the thickening pad 4 and the analysis structure. After the collected blood falls onto the thickening pad 4 through the dripping hole 103, the blood to be tested is absorbed into the analysis structure for testing under the action of the connecting pad.

[0058] It will be apparent to those skilled in the art that the invention is not limited to the details of the exemplary embodiments described above and that the invention can be implemented in other specific forms without departing from the spirit or essential features of the invention. Therefore, the embodiments should be considered exemplary and non-limiting in all respects, and the scope of the invention is defined by the appended claims rather than the foregoing description, and it is intended that all variations falling within the meaning and scope of the equivalent elements of the claims be included in the invention. Any reference numeral in a claim should not be considered as limiting the claim to which it relates.

[0059] In addition, it should be understood that although the present specification is described according to implementation modes, not every implementation mode contains only one independent technical solution. This description of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment may also be appropriately combined to form other implementation modes that can be understood by those skilled in the art.

Claims

1. A chemiluminescent detection kit for thyroglobulin, comprising a box structure, wherein the box structure is composed of a detachably connected upper shell (1) and a lower shell (2) which are sealed together, wherein a droplet measuring hole (103) is provided on the upper shell (1), characterized in that ； It also includes a thickening pad (4) and an analysis structure located inside the box structure, with a gap between the thickening pad (4) and the analysis structure; A plugging component is mounted on the upper shell (1); one end of the plugging component disposed inside the box structure is provided with a connection pad for conducting the thickening pad (4) and the analysis structure; the plugging component is controlled to release the blocking state of the dripping hole (103) and can drive the connection pad to move downward to abut against the thickening pad (4) and the analysis structure.

2. A chemiluminescent detection kit for thyroglobulin according to claim 1, characterized in that: A plurality of upper grooves (104) are provided on the inner wall of the upper shell (1), and a lower convex column (201) adapted to the upper grooves (104) is formed on the inner wall of the lower shell (2), and the lower convex column (201) can be inserted into the upper grooves (104).

3. A chemiluminescent detection kit for thyroglobulin according to claim 1, characterized in that: The analysis structure comprises a gold pad (5), an NC membrane (6) and a water absorbent pad (7); a gap exists between one end of the gold pad (5) and the thickened pad (4); the other end of the gold pad (5) overlaps with one end of the NC membrane (6); and the other end of the NC membrane (6) overlaps with the water absorbent pad (7).

4. A chemiluminescent detection kit for thyroglobulin according to claim 3, characterized in that: A plurality of groups of limit blocks (202) are symmetrically arranged inside the lower shell (2), and the limit blocks (202) are respectively in contact with the thickening pad (4) and the gold pad (5).

5. A chemiluminescent detection kit for thyroglobulin according to claim 3, characterized in that: An observation port (101) is formed on the upper shell (1), the observation port (101) is located directly above the NC film (6), and a transparent lens is sealed and installed at the observation port (101).

6. A chemiluminescent detection kit for thyroglobulin according to claim 1, characterized in that: The upper shell (1) is provided with a mounting hole (102) penetrating the upper shell (1), and a limiting sliding groove (1021) is formed on the inner wall of the mounting hole (102).

7. A chemiluminescent detection kit for thyroglobulin according to claim 6, characterized in that: The blocking assembly comprises a pressing piece (11) arranged axially along the mounting hole (102), a protrusion (1101) adapted to the limiting sliding groove (1021) being formed on the pressing piece (11), one end of the pressing piece (11) extending into the mounting hole (102) being slidably connected to a connecting sleeve (13) rotatably mounted inside the upper shell (1), and an extrusion piece slidably connected to the upper shell (1) being provided at the bottom of the connecting sleeve (13); It also comprises a spring (12), wherein the spring (12) is arranged in the connecting sleeve (13), one end of the spring (12) abuts against the pressing piece (11), and the other end abuts against the inner bottom of the connecting sleeve (13).

8. A chemiluminescent detection kit for thyroglobulin according to claim 7, characterized in that: The connecting sleeve (13) is provided with an abutment plate (14) in an arc-shaped structure, and the abutment plate (14) is formed with a through hole (1401) adapted to the dripping detection hole (103).

9. A chemiluminescent detection kit for thyroglobulin according to claim 7, characterized in that: The extrusion member comprises a pressing plate (9), the pressing plate (9) being slidably arranged in the upper shell (1) via a support rod (8), a sleeve (10) being axially arranged along the connecting sleeve (13) being arranged at the center position of the pressing plate (9), the sleeve (10) being slidably connected to the bottom of the connecting sleeve (13).

10. A chemiluminescent detection method for thyroglobulin, characterized in that: The chemiluminescent detection kit for thyroglobulin according to any one of claims 1 to 9 comprises the following steps: Step 1: Before blood collection, take out the test kit and clean and disinfect the collection site; Step 2: Take out the reagent kit, unpack the packaging on the surface of the reagent kit, and lay the reagent kit flat. At this time, the droplet hole (103) is in a blocked state to prevent external water from entering the box structure through the droplet hole (103) before collection; Step three: by pressing and rotating the blocking structure, the dripping hole (103) can be controlled to release the blocking state, and the connection pad can be driven to move downward to abut against the thickening pad (4) and the analysis structure, and the collected blood is dropped onto the thickening pad (4) through the dripping hole (103). Under the action of the connection pad, the blood to be tested is absorbed into the analysis structure for testing.

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