Artificial breeding method for spinibarbus capito

Abstract

The invention provides an artificial breeding method of spinibarbus capito, which is suitable for Dongting Lake in Hunan Province and four water basins of Hunan, Zi, active yuan and halite, and comprises the steps of parent fish rearing, artificial spawning induction and artificial incubation, and spawning can be carried out for 1-2 times in one year. In the spinibarbus capito parent fish rearing process, puffed floating fish feed is used as a main material and properly matched with green feed, animal bait is added for feeding 2 months before winter, accumulation of fish body fat can be guaranteed so that the fish can smoothly pass the winter, vitamin E, vitamin C and flowing water stimulation are supplemented in the feed before antenatal, and ovary development and ovum maturation can be effectively promoted; the artificial spawning induction adopts a two-needle injection method, the spawning induction efficiency is high, and the fertilization rate is high; through comparison of different hatching devices, the hatching effect of the hatching frame is best, the hatching rate reaches 80% or above, the hatching barrel is inferior, and the small net cage is worst. By means of the artificial propagation method, large-scale production of spinibarbus capito can be achieved, and the method is of great significance in improving the economic benefits of farmers and popularizing the spinibarbus capito artificial propagation technology.

Description

Technical Field

[0001] The invention belongs to the technical field of aquaculture, and in particular relates to an artificial propagation method of spiny barbel. Background Art

[0002] Due to overfishing, habitat destruction and water pollution, the wild resources of spiny barb have suffered serious damage, and the population and distribution range have been severely reduced. It has been listed in the Red List of Endangered Species by the World Conservation Union (IUCN 2013). The distribution range of spiny barb in Hunan has shrunk from the widespread distribution in Dongting Lake and the four river basins of "Xiang, Zi, Yuan and Li" in the 1970s to the Haiyang River, a tributary of the Xiangjiang River, and some sections of the upper reaches of the Yuanjiang River. The number is extremely rare, and the protection of spiny barb resources is imminent. In 2015, the People's Government of Hunan Province included spiny barb in the list of artificially propagated and released species, and the national aquatic germplasm resource protection zone for endemic fish established in the upper reaches of the Yuanjiang River also listed spiny barb as one of its key protection objects. In view of the high nutritional value, good market prospects and breeding benefits of the spiny barb, the development and utilization of resources are gradually emerging in Huaihua, Hunan and other regions, and breeding is gradually heating up. The breeding, reproduction and release of the spiny barb must be supported by its artificial breeding technology.

[0003] Artificial reproduction of fish is to use artificial methods to promote the development and maturity of the testes of male fish and the ovaries of female fish, and to combine the sperm and eggs of fish under artificial conditions through aphrodisiac methods, so as to obtain fertilized eggs, and use certain equipment to make the fertilized eggs develop and hatch into seedlings. The research on artificial reproduction technology of spiny barbel can solve the source of its seedlings and lay a solid foundation for its breeding production, genetic breeding and other work. It is also a necessary prerequisite for carrying out artificial reproduction and release to restore its wild resources and maintain its species genetic diversity. CN103583441A discloses an artificial propagation method of Chinese spiny barb suitable for Chenghai Lake, a plateau lake in Lijiang City, Yunnan Province. CN107182848A discloses an artificial propagation method of Chinese spiny barb suitable for Anhui Province. Zeng Qingxiang disclosed an artificial propagation method of spiny barb suitable for Taojiang waters in Ganzhou City, Jiangxi Province in "Biological Characteristics and Artificial Propagation Technology of Taojiang Spiny Barb". However, since the propagation of spiny barb is greatly affected by the region, the existing propagation method of spiny barb is not ideal when applied to other regions. For example, the induction rate and fertilization rate are low, which makes it difficult to meet the needs of large-scale propagation. Summary of the invention

[0004] The technical problem to be solved by the present invention is to provide an artificial propagation method of spiny barb in view of the above-mentioned deficiencies of the prior art, which can carry out spawning 1 to 2 times in a year. Through artificial propagation, spiny barb can be mass-produced in Dongting Lake, Hunan Province and the Sishui River basin of "Xiang, Zi, Yuan and Li".

[0005] In order to solve the above technical problems, the technical solution adopted by the present invention is:

[0006] A method for artificial propagation of spiny barbel, the method comprising the following steps:

[0007] S1. Broodstock cultivation

[0008] In September of the first year, the spiny barbel parents are transported to the breeding base, and the male and female are mixed in the same pond for breeding. The average weight is 1.2-3.5 kg / tail, and the breeding density is 550-650 kg / mu. They are fed with expanded floating fish feed, and the feeding amount is based on the amount that can be eaten within half an hour; from October to November, they are supplemented with chopped chicken and duck offal, and the daily feeding amount is 1% to 3% of the fish body weight; from December to March of the following year, they are supplemented with malt and vegetable leaves, and the daily feeding amount is 1% of the fish body weight; in mid-to-late May, running water stimulation is used, and 0.01% vitamin E and 0.01% vitamin C are added to the feed to promote the maturation of gonads; feeding is stopped one week before breeding;

[0009] S2. Artificial induction of labor

[0010] From early June to September, the water temperature in the culture pond is maintained at 25.8-27.8℃, pH 7.0-7.5, dissolved oxygen is greater than 5.0mg / L, ammonia nitrogen is less than 0.2mg / L, and nitrite is less than 0.05mg / L. The fish are fed with puffed floating feed, and the amount of feed should be consumed within half an hour;

[0011] S201, Oxytocic injection

[0012] Female fish received the first injection, with a dose of luteinizing hormone-releasing hormone LRH-A2 1-3 μg / kg fish body, carp pituitary PG 1-3 mg / kg fish body, and male fish were not injected; 12 hours after the first injection, female fish received the second injection, with a dose of luteinizing hormone-releasing hormone LRH-A2 5-10 μg / kg fish body, carp pituitary PG 1-3 mg / kg fish body, dioicosanoid maleate DOM 3-10 mg / kg fish body, and chorionic gonadotropin HCG 500-1500 IU / kg fish body, and male fish were injected with half the dose of female fish;

[0013] S202, insemination

[0014] After the induction of spawning, female and male fish with mature gonads were selected for artificial insemination. First, 0.8% saline or Ren's saline was added to the container, and then the female fish eggs and male semen were squeezed out separately to mix the sperm and eggs. After 1 minute, a debonding agent was added for debonding.

[0015] S3. Artificial incubation

[0016] After the fertilized eggs are debonded, they are incubated in an incubator. The fertilized eggs of the same fish are placed in the same device. The incubation water temperature is 25.5-28.5°C, the pH is 7-8, and the dissolved oxygen is maintained above 6 mg / L.

[0017] Preferably, the crude protein content of the puffed floating fish feed in S1 is ≥32%, the crude fat content is 4.5%-5.5%, and the particle size is 7.0 mm.

[0018] Preferably, the artificial injection of oxytocin in S2 is performed once from early June to mid-July and once from late August to mid-September.

[0019] Preferably, the first injection dose of the female fish in S201 is 3 μg / kg of luteinizing hormone releasing hormone LRH-A2 and 1 mg / kg of carp pituitary PG.

[0020] Preferably, the second injection dose of the female fish in S201 is luteinizing hormone releasing hormone LRH-A2 10 μg / kg fish body, carp pituitary PG 2 mg / kg fish body, dioicosanoid maleate DOM 3 mg / kg fish body and human chorionic gonadotropin HCG 500 IU / kg fish body.

[0021] Preferably, during the artificial insemination in S202, the ratio of the number of female fish to that of male fish is 1.5 to 2.6:1.

[0022] Preferably, the debonding agent in S202 is a 25 g / L talcum powder aqueous solution. After the fertilized egg is completely debonded, the talcum powder adhering to the surface of the fertilized egg needs to be removed until the fertilized egg becomes light yellow.

[0023] Preferably, the incubation device in S3 comprises a small mesh cage, an incubation frame or an incubation bucket.

[0024] The present invention has the following significant technical effects:

[0025] 1. The present invention provides an artificial propagation method of spiny barb suitable for Dongting Lake in Hunan Province and the Sishui River Basin of "Xiang, Zi, Yuan, and Li". The peak propagation period of spiny barb is from early June to September, and spawning can be carried out 1 to 2 times in a year. In the cultivation of spiny barb broodstock, puffed floating fish feed is mainly used, and green feed is appropriately matched. Two months before winter, animal bait is added to feed, which can ensure the accumulation of fish body fat so that it can pass the winter smoothly. Vitamin E and vitamin C are supplemented in the prenatal feed and water flow stimulation is performed, which can effectively promote ovarian development and egg maturation; artificial induction of labor adopts a two-needle injection method, the use of induction agent is scientific and reasonable, the induction efficiency is high, the fertilization rate is high, and the success rate of artificial propagation of spiny barb is effectively improved; the present invention also compares different incubation devices and finds that the incubation effect of the incubation frame is the best, and the hatching rate reaches more than 80%, followed by the incubation bucket, and the small net cage is the worst.

[0026] 2. The artificial propagation method of the present invention can enable the scaled production of spiny barb, improve the economic benefits of breeders, solve the source of its seedlings, lay a solid foundation for aquaculture production, genetic breeding and the like, and also provide a basis for carrying out its artificial proliferation and release to restore wild resources and maintain species genetic diversity.

[0027] The present invention is further described in detail below with reference to the accompanying drawings and embodiments. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 is the distribution diagram of the egg diameter of the spiny barbel in Example 2;

[0029] Figure 2 This is a microscope picture of the spiny barbel eggs of Example 2. DETAILED DESCRIPTION

[0030] Source of spiny barb parents: wild individuals of spiny barb and their first-generation individuals in the Hongjiang section of Huaihua in the Yuanjiang River Basin.

[0031] The oxytocic agent of the present invention comprises: carp pituitary gland (PG), with a specification of 1 mg / piece; human chorionic gonadotropin (HCG), with a specification of 5000 IU / tube, produced by Ningbo No. 2 Hormone Factory; luteinizing hormone-releasing hormone A2 (LRH-A2), with a specification of 125 μg / tube, produced by Ningbo No. 2 Hormone Factory; and dioxinone maleate (DOM), with a specification of 100 mg / tube, produced by Ningbo Sansheng Pharmaceutical Co., Ltd. During preparation, the carp pituitary gland is ground into powder, all the oxytocic agents are added, and then a suspension is prepared for injection with physiological saline or Ringer's physiological saline with a mass fraction of 0.8%.

[0032] Example 1

[0033] This embodiment is a method for artificial propagation of spiny barb, which is carried out in Changsha, Hongjiang and Chaling, Hunan, respectively, and specifically comprises the following steps:

[0034] S1. Broodstock cultivation

[0035] In September 2021, the spiny barbel parents were transported to the cage breeding base. The cage specifications (length × width × height) were 4m × 4m × 4m, and the mesh size of the inner layer was 5cm and the outer layer was 10cm. Before being put down from the box, soak them in a 2.5% saline solution for 10 minutes. For severely injured individuals, intrathoracic injection of sodium penicillin and glucose injection is used, with a dose of 100,000 IU / kg fish body. Male and female fish are mixed in the same pond, with an average weight of 1.2-3.5 kg / tail and a breeding density of 550 kg / mu. Haida brand puffed floating fish feed (crude protein content ≥32%, crude fat content 4.5%-5.5%, particle size 7.0 mm) is used for feeding, and the feeding amount is based on the consumption within half an hour; from October to November, chopped chicken and duck offal are supplemented with 1% of body weight per day; from December to March 2022, malt and vegetable leaves are supplemented with 1% of fish body weight per day; in mid-to-late May, running water stimulation is used, and 0.01% vitamin E and 0.01% vitamin C are added to the feed to promote gonadal maturation; feeding is stopped one week before breeding.

[0036] S2. Artificial induction of labor

[0037] From early June to September, the water temperature is maintained at 25.8-27.8℃, pH 7.0-7.5, dissolved oxygen is greater than 5.0mg / L, ammonia nitrogen is less than 0.2mg / L, nitrite is less than 0.05mg / L, and puffed floating fish feed is fed. The feeding amount should be based on the consumption within half an hour.

[0038] S201, Oxytocic injection

[0039] At around 8 am, female fish received the first injection, with a dose of luteinizing hormone-releasing hormone LRH-A2 1μg / kg fish body and carp pituitary PG 3mg / kg fish body; male fish did not receive injection. At around 8 pm, female fish received the second injection, with a dose of luteinizing hormone-releasing hormone LRH-A2 8μg / kg fish body, carp pituitary PG 3mg / kg fish body, maleic acid dioxin DOM 10mg / kg fish body and chorionic gonadotropin HCG 1500IU / kg fish body, and male fish received half the dose of female fish.

[0040] S202, insemination

[0041] After the induction of labor, select female and male fish with mature gonads, strong physique and no body injuries for artificial insemination. First, pour a small amount of 0.8% saline into the container, then squeeze out the female fish eggs and male semen respectively to mix the sperm and eggs. After 1 minute, add 25g / L talcum powder aqueous solution to debond, gently stir the water with goose feathers, and repeatedly add new water to rinse. After the fertilized eggs are completely debonded, remove the talcum powder adhering to the surface of the fertilized eggs until the fertilized eggs are light yellow.

[0042] S3. Artificial incubation

[0043] After the fertilized eggs are debonded, they are incubated in small net cages. The small net cages are made of nylon, with a size of 60cm×60cm×40cm and a mesh size of 80. The small net cages are fixed with a fir wood frame. Each frame fixes 3 small net cages, 20cm apart from each other, to facilitate water exchange. The four corners of the bottom of the small net cage are tied with stones to fix them. The box mouth is 20cm above the water surface. The fir wood frame is placed on the lake surface of the net cage breeding base. The oxygen pump is continuously oxygenated for 24 hours with a power of 80W. 3,000 fertilized eggs are placed in each small net cage. The fertilized eggs of the same fish are placed in the same small net cage for incubation. The incubation water temperature is 26-27.5℃, the pH is 7.3-8, and the dissolved oxygen is maintained above 6.3mg / L. The incubation is 48h. When the sunlight is strong, cover it with a sunshade cloth to prevent direct sunlight from affecting the incubation of the fertilized eggs. Record the incubation water temperature every 2h, and remove the necrotic eggs in time.

[0044] The results of artificial propagation of spiny barb in this example are shown in Table 1.

[0045] Table 1 Results of artificial propagation of spiny barbel in 2022

[0046]

[0047] Example 2

[0048] This embodiment is a method for artificial propagation of spiny barb, which is carried out in Hongjiang and Chaling, Hunan, and specifically comprises the following steps:

[0049] S1. Broodstock cultivation

[0050] In September 2022, the spiny barbel parents were transferred to the cage culture base and soaked in 20mg / L potassium permanganate solution for 30 minutes before being put into the cage. Severely injured individuals were injected with sodium penicillin and glucose injection in the chest, with a dose of 100,000 IU / kg fish body. Male and female fish were mixed in the same pond, with an average weight of 1.2-3.5kg / fish. The stocking density was 600kg / mu, and Haida brand puffed floating fish feed (crude protein content ≥32%, crude fat content 4.5%-5 .5%, particle size 7.0mm) for feeding, the feeding amount is based on the consumption within half an hour; from October to November, supplement with chopped chicken and duck offal, the daily feeding amount is 2% of the fish body weight; from December to March 2023, supplement with malt and vegetable leaves, the daily feeding amount is 1% of the fish body weight; in mid-to-late May, use running water stimulation, add 0.01% vitamin E and 0.01% vitamin C to the feed to promote gonadal maturation; stop feeding one week before breeding.

[0051] S2. Artificial induction of labor

[0052] From early June to September, the water temperature is maintained at 25.8-27.8℃, pH 7.0-7.5, dissolved oxygen is greater than 5.0mg / L, ammonia nitrogen is less than 0.2mg / L, nitrite is less than 0.05mg / L, and puffed floating fish feed is fed. The feeding amount should be based on the consumption within half an hour.

[0053] S201, Oxytocic injection

[0054] At around 8 am, female fish received the first injection, with a dose of luteinizing hormone-releasing hormone LRH-A2 2μg / kg fish body and carp pituitary PG 2mg / kg fish body; male fish did not receive injection. At around 8 pm, female fish received the second injection, with a dose of luteinizing hormone-releasing hormone LRH-A2 5μg / kg fish body, carp pituitary PG 1mg / kg fish body, dioxin maleate DOM 6mg / kg fish body and chorionic gonadotropin HCG 1000IU / kg fish body; male fish received half the dose of female fish.

[0055] S202, insemination

[0056] After the induction of labor, select female and male fish with mature gonads, strong physique, and no body injuries for artificial insemination. First, pour a small amount of Ren's saline into the container, then squeeze out the female fish eggs and male semen respectively to mix the sperm and eggs. After 1 minute, add 25g / L talcum powder aqueous solution to debond, gently stir the water with goose feathers, and repeatedly add new water to rinse. After the fertilized eggs are completely debonded, remove the talcum powder adhering to the surface of the fertilized eggs until the fertilized eggs are light yellow.

[0057] S3. Artificial incubation

[0058] After the fertilized eggs are debonded, they are incubated in an incubation bucket made of stainless steel, with a screen made of nylon and a mesh size of 60 mesh. The water in the incubation bucket is mountain spring water near the base. After filtration, the water enters from the bottom and exits from the top. The fertilized eggs are always in a slow rolling state in the bucket. The oxygen pump is oxygenated 24 hours a day with a power of 80W. 3,000 fertilized eggs are placed in each incubation bucket. The fertilized eggs of the same fish are placed in the same incubation bucket for incubation. The incubation water temperature is 26.5-28.2℃, the pH is 7.3-7.8, and the dissolved oxygen is maintained above 6.5mg / L. The incubation is 48h. When the sunlight is strong, cover it with a sunshade cloth to prevent direct sunlight from affecting the hatching of the fertilized eggs. Record the incubation water temperature every 2 hours, and remove necrotic eggs in time.

[0059] The results of artificial propagation of spiny barb in this example are shown in Table 2.

[0060] Table 2 Results of artificial propagation of spiny barbel in 2023

[0061]

[0062] In this embodiment, the spiny barb was dissected at the beginning of the breeding season. It was found that the spiny barb had large eggs and a small number of eggs. The number of eggs carried by an individual weighing 1975 g was only 37,599. The egg diameter distribution diagram is as follows: Figure 1 As shown in the figure, there are two obvious peaks, which indicates that the spiny barb can spawn at least twice a year. The diameter of the eggs in the first peak is between 0.51 and 1.43 mm, with an average of 0.97 mm. The number of eggs is relatively small, accounting for 37.9% of the total number of eggs. The diameter of the eggs in the second peak is between 1.84 and 2.86 mm, with an average of 2.19 mm. The number of eggs is relatively large, accounting for 62.1% of the total number of eggs. The two types of eggs also have different appearances, such as Figure 2 As shown, eggs with larger diameters are mostly round or oval, full and light yellow, mostly free, with smooth and transparent egg membranes and good maturity ( Figure 2 The eggs with smaller diameters are slightly irregular in shape, not free, and wrapped in ovarian tissue, with a grayish-white color ( Figure 2 (Indicated by white arrows). This shows that the egg development of the spiny barb is not synchronous, indicating that it is a fish that spawns in batches.

[0063] Example 3

[0064] This embodiment is a method for artificial propagation of spiny barb, which is carried out in Chaling and Hongjiang, Hunan, and specifically comprises the following steps:

[0065] S1. Broodstock cultivation

[0066] In September 2023, the spiny barbel parents were transferred to the cage culture base and soaked in a 2.5% saline solution for 10 minutes before being put into the cage. Severely injured individuals were injected with sodium penicillin and glucose injection in the chest, with a dose of 100,000 IU / kg fish body. Male and female fish were mixed in the same pond, with an average weight of 1.2-3.5 kg / fish. The stocking density was 650 kg / mu, and Haida brand puffed floating fish feed (crude protein content ≥32%, crude fat content 4.5%~ 5.5%, particle size 7.0mm) for feeding, the feeding amount is based on the consumption within half an hour; from October to November, supplement with chopped chicken and duck offal, the daily feeding amount is 3% of the fish body weight; from December to March 2024, supplement with malt and vegetable leaves, the daily feeding amount is 1% of the fish body weight; in mid-to-late May, use running water stimulation, add 0.01% vitamin E and 0.01% vitamin C to the feed to promote gonadal maturation; stop feeding one week before breeding.

[0067] S2. Artificial induction of labor

[0068] From early June to September, the water temperature is maintained at 25.8-27.8℃, pH 7.0-7.5, dissolved oxygen is greater than 5.0mg / L, ammonia nitrogen is less than 0.2mg / L, nitrite is less than 0.05mg / L, and puffed floating fish feed is fed. The feeding amount should be based on the consumption within half an hour.

[0069] S201, Oxytocic injection

[0070] At around 8 am, female fish received the first injection, with a dose of luteinizing hormone-releasing hormone LRH-A2 3μg / kg fish body and carp pituitary PG 1mg / kg fish body; male fish did not receive injection. At around 8 pm, female fish received the second injection, with a dose of luteinizing hormone-releasing hormone LRH-A2 10μg / kg fish body, carp pituitary PG 2mg / kg fish body, dioxin maleate DOM 3mg / kg fish body and chorionic gonadotropin HCG 500IU / kg fish body, and male fish received half the dose of female fish.

[0071] S202, insemination

[0072] After the induction of labor, select female and male fish with mature gonads, strong physique and no body injuries for artificial insemination. First, pour a small amount of 0.8% saline into the container, then squeeze out the female fish eggs and male semen respectively to mix the sperm and eggs. After 1 minute, add 25g / L talcum powder aqueous solution to debond, gently stir the water with goose feathers, and repeatedly add new water to rinse. After the fertilized eggs are completely debonded, remove the talcum powder adhering to the surface of the fertilized eggs until the fertilized eggs are light yellow.

[0073] S3. Artificial incubation

[0074] After the fertilized eggs are debonded, they are incubated in an incubation frame. The incubation frame is a rectangular parallelepiped with a flat bottom. The frame is made of fir strips, made of nylon, with a size of 80cm×40cm×30cm and a mesh size of 80. The incubation frame is fixed with a fir strip square frame (size: 2.2m×0.7m). Three incubation frames are fixed in each square frame, 20cm apart from each other, to facilitate water exchange. The four corners of the bottom are tied with stones to fix them. The frame mouth is 20cm above the water surface. The fir strip square frame is placed on the lake surface of the cage breeding base. The oxygen pump is continuously oxygenated for 24 hours with a power of 80W. 3,000 fertilized eggs are placed in each incubation frame. The fertilized eggs of the same fish are placed in the same incubation frame for incubation. The incubation water temperature is 26-27.5℃, the pH is 7.2-8, the dissolved oxygen is maintained at more than 6.3mg / L, and the incubation is 48h. When the sunlight is strong, cover it with a sunshade cloth to prevent direct sunlight from affecting the incubation of the fertilized eggs. Record the incubation water temperature every 2 hours and remove necrotic eggs in time.

[0075] The results of artificial propagation of spiny barb in this example are shown in Table 3.

[0076] Table 3 Results of artificial propagation of spiny barbel in 2024

[0077]

[0078] The results of Examples 1 to 3 show that the hatching effect of the hatching frame is the best, followed by the hatching bucket, and the small net cage is the worst. The hatching frame is a rectangular parallelepiped with a flat bottom, and oxygen is supplied by an oxygen pump, which effectively avoids the death of fertilized eggs due to lack of oxygen caused by the uneven bottom of the small net cage, and can also adjust the depth of the fertilized eggs in the water; the hatching bucket can simulate the hatching conditions of fertilized eggs of fish that lay floating eggs, and due to the effect of water flow, the fertilized eggs are in a rolling state in the water body.

[0079] The artificial propagation method of the spiny barb is suitable for the Yuanjiang River Basin in Hunan Province. The spiny barb is a fish that lays eggs in batches. The peak breeding period is from early June to September, and 1 to 2 spawning times can be carried out in a year. The artificial propagation method of the present invention can enable the spiny barb to be produced on a large scale, which is of great significance for improving the economic benefits of farmers and promoting the artificial propagation technology of the spiny barb in different regions.

[0080] The above is only a preferred embodiment of the present invention and does not limit the present invention in any way. Any simple modification, change and equivalent change made to the above embodiment according to the technical essence of the invention still falls within the protection scope of the technical solution of the present invention.

Claims

1. A method for artificial propagation of spiny barb, characterized in that: The method comprises the following steps: S1. Broodstock cultivation In September of the first year, the spiny barbel parents are transported to the breeding base, and the male and female are mixed in the same pond for breeding. The average weight is 1.2-3.5 kg / tail, and the breeding density is 550-650 kg / mu. They are fed with expanded floating fish feed, and the feeding amount is based on the amount that can be eaten within half an hour; from October to November, they are supplemented with chopped chicken and duck offal, and the daily feeding amount is 1% to 3% of the fish body weight; from December to March of the following year, they are supplemented with malt and vegetable leaves, and the daily feeding amount is 1% of the fish body weight; in mid-to-late May, running water stimulation is used, and 0.01% vitamin E and 0.01% vitamin C are added to the feed to promote the maturation of gonads; feeding is stopped one week before breeding; S2. Artificial induction of labor From early June to September, the water temperature in the culture pond is maintained at 25.8-27.8℃, pH 7.0-7.5, dissolved oxygen is greater than 5.0mg / L, ammonia nitrogen is less than 0.2mg / L, and nitrite is less than 0.05mg / L. The fish are fed with puffed floating feed, and the amount of feed should be consumed within half an hour; S201, Oxytocic injection Female fish received the first injection, with a dose of luteinizing hormone-releasing hormone LRH-A2 1-3 μg / kg fish body, carp pituitary PG 1-3 mg / kg fish body, and male fish were not injected; 12 hours after the first injection, female fish received the second injection, with a dose of luteinizing hormone-releasing hormone LRH-A2 5-10 μg / kg fish body, carp pituitary PG 1-3 mg / kg fish body, dioicosanoid maleate DOM 3-10 mg / kg fish body, and chorionic gonadotropin HCG 500-1500 IU / kg fish body, and male fish were injected with half the dose of female fish; S202, insemination After the induction of spawning, female and male fish with mature gonads were selected for artificial insemination. First, 0.8% saline or Ren's saline was added to the container, and then the female fish eggs and male semen were squeezed out separately to mix the sperm and eggs. After 1 minute, a debonding agent was added for debonding. S3. Artificial incubation After the fertilized eggs are debonded, they are incubated in an incubator. The fertilized eggs of the same fish are placed in the same device. The incubation water temperature is 25.5-28.5°C, the pH is 7-8, and the dissolved oxygen is maintained above 6 mg / L.

2. A method for artificial propagation of spiny barb according to claim 1, characterized in that: S1 The puffed floating fish feed has a crude protein content of ≥32%, a crude fat content of 4.5% to 5.5%, and a particle size of 7.0 mm.

3. A method for artificial propagation of spiny barb according to claim 1, characterized in that: The artificial injection of oxytocin described in S2 is performed once from early June to mid-July and once from late August to mid-September.

4. A method for artificial propagation of spiny barb according to claim 1, characterized in that: The first injection dose of the female fish in S201 is 3 μg / kg of luteinizing hormone releasing hormone LRH-A2 and 1 mg / kg of carp pituitary PG.

5. A method for artificial propagation of spiny barb according to claim 1, characterized in that: The second injection dose of the female fish in S201 is luteinizing hormone releasing hormone LRH-A2 10 μg / kg fish body, carp pituitary PG 2 mg / kg fish body, dioicosanoid maleate DOM 3 mg / kg fish body and human chorionic gonadotropin HCG 500 IU / kg fish body.

6. A method for artificial propagation of spiny barb according to claim 1, characterized in that: S202 During artificial insemination, the ratio of the number of female fish to that of male fish is 1.5 to 2.6:

1.

7. A method for artificial propagation of spiny barb according to claim 1, characterized in that: S202 The debonding agent is a 25 g / L talcum powder aqueous solution. After the fertilized egg is completely debonded, the talcum powder adhering to the surface of the fertilized egg needs to be removed until the fertilized egg becomes light yellow.

8. A method for artificial propagation of spiny barb according to claim 1, characterized in that: S3 The hatching device comprises a small mesh cage, a hatching frame or a hatching bucket.

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